Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 94,850 | T→C | 3.3% | V67V (GTT→GTC) | murF → | UDP‑N‑acetylmuramoyl‑tripeptide:D‑alanyl‑D‑ alanine ligase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 94,850 | 0 | T | C | 3.3% | 428.4 / 2.5 | 134 | V67V (GTT→GTC) | murF | UDP‑N‑acetylmuramoyl‑tripeptide:D‑alanyl‑D‑ alanine ligase |
Reads supporting (aligned to +/- strand): ref base T (64/63); new base C (3/2); total (68/66) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-01 |
Only 100 of 134 total aligned reads displayed.
CCATGAAGATTACCAGATTGTTGGCAATCAGCGTCTGGACTACTCCGATCGCGTCACGGTGGCGCGTCTGCTGGGGGTGATTGCATGATTAGCGTAACCCTTAGCCAACTTACCGACATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACC > NC_000913/94566‑95136 | ccATGAAGATTACCAGATTGTTGGCAATCAGCGTCTGGACTACTCCGATCGCGTCACGGTGGCGCGTCTGCTGGGGGTGATTGCATGATTAGCGTAACCCTTAGCCAACTTACCGACATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAg < 1:744752/287‑1 (MQ=255) tGAAGATTACCAGATTGTTGGCAATCAGCGTCTGGACTACTCCGATCGCGTCACGGTGGCGCGTCTGCTGGGGGTGATTGCATGATTAGCGTAACCCTTAGCCAACTTACCGACATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCACACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGAACTACTGGTTAGCCGt > 1:582836/1‑288 (MQ=255) ttACCAGATTGTTGGCAATCAGCGTCTGGACTACTCCGATCGCGTCACGGTGGCGCGTCTGCTGGGGGTGATTGCATGATTAGCGTAACCCTTAGCCAACTTACCGACATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCt < 1:962032/287‑1 (MQ=255) ttACCAGATTGTTGGCAATCAGCGTCTGGACTACTCCGATCGCGTCACGGTGGCGCGTCTGCTGGGGGTGATTGCATGATTAGCGTAACCCTTAGCCAACTTACCGACATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGAGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAAGCGAAAGCTGGCGGCGCAGGCGCAATACTGGTTAGCCGTCCGCt < 1:700219/287‑1 (MQ=255) ttgGCAATCAGCGTCTGGACTACTCCGATCGCGTCACGGTGGCGCGTCTGCTGGGGGTGATTGCATGATTAGCGTAACCCTTAGCCAACTTACCGACATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCt > 1:324666/1‑288 (MQ=255) gCAATCCGCGTCGGGTCTACTCCGGGCGCGTCACGGTGGCGCTGCTGATGGGGGTGATTGCAAGATGAGCGTAACCCTTAGCCAACTTACCGACATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGcc < 2:98158/288‑1 (MQ=255) gTCTGGACTACTCCGATGGCGTCACGGTGGCGCGTCGGCTGGGGGTGATTGCATGATTAGCGTAACCCTTAGCCAACTTACCGACATTCTCACCGGTGAACTGCAAGGTGCAGATAGCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑GTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTaa < 1:976677/287‑1 (MQ=255) actCCGATCGCGTCACGGTGGCGCGTCTGCTGGGGGTGATTGCATGATTAGCGTAACCCTTAGCCAACTTACCGACATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCCCTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGAt > 1:406570/1‑289 (MQ=255) actCCGATCGCGTCACGGTGGCGCGTCTGCTGGGGGTGATTGCATGATTAGCGTAACCCTTAGCCAACTTACCGACATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGAt > 1:80527/1‑289 (MQ=255) aTCGCGTCACGGTGGCGCGTCTGCTGGGGGTGATTGCATGATTAGCGTAACCCTTAGCCAACTTACCGACATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCCCTCATGGGCAACCGTCCGCTGGACATCG‑CCCCGCAGCAGTTTATCCTTAGGGATAC‑GCGt > 1:327153/1‑289 (MQ=255) aTCGCGTCACGGTGGCGCGTCTGCTGGGGGTGATTGCATGATTAGCGTAACCCTTAGCCAACTTACCGACATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAc‑gcg > 1:157704/1‑288 (MQ=255) aTCGCGTCACGGTGGCGCGTCTGCTGGGGGTGATTGCATGATTAGCGTAACCCTTAGCCAACTTACCGACATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCCCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACATGCCGCAGTTAAGCGTCACGGATAc‑gcg > 2:282767/1‑288 (MQ=255) aCGGTGGCGCGTCTGCTGGGGGTGATTGCATGATTAGCGTAACCCTTAGCCAACTTACCGACATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGtt > 2:721835/1‑289 (MQ=255) cgcgggCGCGTCTGCTGGGGGTGAGTGCATGATTTGCGTAGCCCTTTGCCAACTTACCGACATTCTCAACAGTTAACTGCAAAGGGCAGATATCACCCTGGATGCTTTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGGTGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGttt < 2:836034/285‑1 (MQ=255) cgcgTCTGCCGGGGGTGAATGCATGATTAGCGTAACCCTTAGCCAACTTACCGACATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGACTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCCAGCGCACTACTGGTTAGCCGCCCGCCGGCCATCG‑ACCTGCCGCAGTTAATCGTCAAGGCTAC‑GCGTCCGGCGTTGGGTGa > 2:1026329/1‑288 (MQ=255) gcgTCTGCTGGGGGTGATTGCATGATTAGCGTAACCCTTAGCCAACTTACCGACATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGaa < 1:641905/288‑1 (MQ=255) tgGGGGTGATTGCATGATTAGCGTAACCCTTAGCCAACTTACCGACATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGc < 1:424515/288‑1 (MQ=255) aTTGCATGATTAGCGTAACCCTTAGCCAACTTACCGACATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑AACTGCCGCAGTTAATCGTCACGGATAC‑GCGTCTGGCGTTTGGTTAACTGGCTGCATGGGTTc > 1:503210/1‑288 (MQ=255) cATGATTAGCGTAACCCTTAGCCACCTTCCCGACATTCCCAACGGTTAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGAAACCCGAAAACTGACGCGGGGCTCCCTGTTTGTTGCCCTGAAAGGCGAACGA‑TTTGACGCCCACGATTTTGCCTACCAGCCGAAAGCTGGCGGCGCAGGCGCCCTACGGATTAGCCCTCAGCTGGTCAACGCACTTGCGGCGGTT‑ATCGTCAAGCATACAGCG‑CTGTCCGTTGGTGAACTgcgctcattgcgtaggcc > 2:965142/1‑271 (MQ=255) tGATTAGCGTAACCCTTAGCCAACTTACCGACATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCa > 1:994628/1‑289 (MQ=255) ccTTAGCCAACTTACCGACATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTCAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCa < 1:1113475/273‑1 (MQ=255) aGCCAACTTACCGACATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTgg > 2:69065/1‑289 (MQ=255) aGCCAACTTACCGACATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTCGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAAGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGCCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTgg > 1:840853/1‑289 (MQ=255) aCTTACCGACATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGct < 1:244950/289‑1 (MQ=255) cTTACCGACATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCCGGCGCACTACTGGTCAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGCTAC‑GGGCCTGGCGTTTGGTCAACTGGCGGCAAGGGTTCGGCAGCAAGTTCCGgctgtcccgtatagac > 2:1113475/1‑275 (MQ=255) cTTACCGACATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGc > 1:840589/1‑287 (MQ=255) cTTACCGACATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGc > 1:202443/1‑287 (MQ=255) cTTACCGACATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGc > 1:803500/1‑287 (MQ=255) cTTACCGACATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAAGCGTCCAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTTCGGGGCGGGTGGTTGc > 1:908277/1‑287 (MQ=255) tctcAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGGGGTTGCTCTGACGGGGt > 1:33210/1‑287 (MQ=255) aaCTGCATGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCaaaa > 1:16374/1‑288 (MQ=255) aaCTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTc < 2:634737/194‑1 (MQ=255) aaCTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCaaaa > 2:231067/1‑288 (MQ=255) ggTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTg > 1:581558/1‑256 (MQ=255) ggTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTg < 2:581558/256‑1 (MQ=255) tGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTaaa > 1:420773/1‑289 (MQ=255) aGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAagag > 1:726678/1‑289 (MQ=255) aCCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGttt > 1:196056/1‑195 (MQ=255) aCCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTACAGAGATGAcg > 1:621562/1‑288 (MQ=255) aCCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGAcg > 1:1146624/1‑288 (MQ=255) aCCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTCAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAAc < 2:819992/269‑1 (MQ=255) aCCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTGTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGttt < 2:196056/195‑1 (MQ=255) aCCCTTGATGCTGTAACCACTGATACCCGAAAAATGACGCCGGGCTGCCTGTTTGTTGCACTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGACCTACTGGTTAGCCGTCCGCTGGACATCG‑ACGTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGCTTGGTGAACTGGATGCATGGGGTAGCCAGAAAGTTCCGGCGCGCGCGGTTGCTGGTACGGGGTCCTCCGGCAAAAGCTCCTTTAAAGCGATggagg > 2:943711/1‑285 (MQ=255) ttGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACggcggc < 2:157704/289‑1 (MQ=255) tGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACggcggc > 1:211075/1‑288 (MQ=255) tcgttcagcACTANTACCAGAAAACGTCCGCCGGGCTGGTTGTTTGTAGCCATGAAAGGCGAACGTGTTTGA‑CCCCACAAGTTTGCCGAGCAGGCGAAAGCTGGCGGCGCAGGCGCTCTACAGGTTAGCCGTCCGCTGGACATCG‑ACCGGCCGCAGTTAATCGTCAAGGAGAC‑TCGTGTGGCGTTGGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGAttt < 2:459652/281‑1 (MQ=255) gTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGATTGGGGAACTGGCTGCATGGGTTCGCCCGCACGCTCCGGCCCGCGTGGTTGCCCTTACGGGGTCCTCCGGAAAAACCTCCGTTACAGAGATGACGGAGGCGGTTTTaa > 2:1147502/1‑289 (MQ=255) gTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGCCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTTCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGGTTTTaa > 1:537457/1‑289 (MQ=255) cACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAg < 1:69065/289‑1 (MQ=255) aCTGATACCCGAAAACTGACGACGTGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAGTCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTTGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTaaa < 1:898761/261‑1 (MQ=255) cggATACCCGAAAACTGGCGCCGGGCTGCCTGTTTCTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATCTTGCCGACCAGGCGAAAGCTGGGGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCGGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTg < 2:16374/287‑1 (MQ=255) tGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAg < 1:123129/286‑1 (MQ=255) tGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAg > 2:123129/1‑286 (MQ=255) tGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGc > 2:922744/1‑289 (MQ=255) tGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGc > 2:259003/1‑289 (MQ=255) gATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCg > 1:375361/1‑289 (MQ=255) ccGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCAATTTTAAGCCAGTGCGGCAac < 1:969183/289‑1 (MQ=255) ccGAAAACGGACGCCGGGCTGCCTGTTTGTGGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAac < 1:1147502/289‑1 (MQ=255) aCGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTa < 2:306689/132‑1 (MQ=255) gCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTGCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATAc < 2:500151/288‑1 (MQ=255) ggCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGACCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑AACTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGACAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAACGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACggcag > 1:676576/1‑289 (MQ=255) gCTGCCTGTTTGTTGCCCTGAAAAGCGAACGT‑TTTGATGCCCACGAGTTTGCCGACCAGTCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCTTCCGCGTGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTTGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGTGGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGTCAACACGCTGTATACggcag < 2:456846/288‑1 (MQ=255) ccTGTTGGTTGCCCTGAAAGGGGAACGT‑TTTGATGCCCACGAGTGTGCCTGCCAGGCGAAAGCTGGCGGCGCAGGCACACTCCTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCTCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGCGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAAt < 2:80527/289‑1 (MQ=255) tttgttGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAACACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCCca > 1:466925/1‑289 (MQ=255) ttgttgCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTcaa > 1:799982/1‑289 (MQ=255) gCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACg > 1:738161/1‑289 (MQ=255) gCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGAAGGCAATCTCACAAACg > 2:676209/1‑289 (MQ=255) cccTGGAATGCGAACGT‑GTTGAGGCCCACGATTTTGCCGACCAGGCGAAAGCTGGTGGGGCAGGCGCACTACTGGTTAGCCGTACTCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGa < 2:991561/289‑1 (MQ=255) tGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACAt < 1:259003/289‑1 (MQ=255) tGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAAACGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGGCCTCCGGCAAAACCTCGGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCACCCCGCTTTATACGGCAGGCAATCTcaacaaacaca > 2:116419/1‑283 (MQ=255) tGAAAGGCGAACGT‑TTTGATGCCCACGATGTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGACGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCGGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACAt < 1:379968/289‑1 (MQ=255) aaaGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGGCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCCCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATCTTCAGCCAGTGCGGCAACCCGCCTTATACGGCCGGCAACCTCaacaaccaaagcg > 2:362566/1‑282 (MQ=255) tcaaaCGT‑TTGGATGCCCACGATTTTGCGGACAAGGGGAAGGCTGGCGGCGCAGGCGCACTACGGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGgtg < 2:1146624/285‑1 (MQ=255) cGAACGT‑TTGGATGCCCACGATTTTGCCGACCAGGCGAAGGGGGTGGGCGGGGGCGAATGACTGGTTAGCCGTCCGCTGGAGATCG‑ACCTGCCGCAGTTAATGGTCAGGGATAC‑GCGTCTGGCGTTTGGTGACCTGGCTGCATGGGTTCGCCAGCAAGTTCCGGGGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGgtgt < 2:33210/288‑1 (MQ=255) gAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTAc < 1:676209/289‑1 (MQ=255) gAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGAACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTa < 2:420773/288‑1 (MQ=255) t‑tttGAGGCCCACGATTTTGCCGACCAGGCGAAAGCGGGCGGCGCAGGCGCAGTACTGGTTAGCCGTCAGCTGGACATCG‑ACTTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATggg < 2:855184/140‑1 (MQ=255) tttGAGTCCCCCGATTTTGCCGGCCAGGCGAAAGGTGGCGGGGCAGGCGGACTACTGGTTAACCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGa < 2:799982/289‑1 (MQ=255) ctaCGATTTTTCCGACCAGGCGAAAGCTGGCGGGTCAGGCGCACTACTGGTTATCCGTCCGCTGGCCATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGtt < 1:943711/286‑1 (MQ=255) cgccagttttGCCGACCAGGGGAGAGCGGACGGGACGTGCGCATGCCTGGTTAGCGTTCCGGTGGAAATCG‑ACCTGCCGCAGTCAATCTTCAGGAATAG‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTTGGCAGCAATTGCCGGCGCGCGTGGTTGCGCTGACGGGGCCCTCCGGCAAACCCTCCGTAAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTAAACGGCAGGCAATCTCCACAACGACATCGGTGTACCGATGACGCGGtt < 2:537457/282‑1 (MQ=255) cACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCATGCGCACTACTGGTTCGCCGTCCGCTGGACATCG‑GCCTGCCGCAAGTAAGCGTCCAGGATTC‑ACCTCGGGCGTTTGGTGAACTGGCTGCCTGGGTTCGCCAGcc > 1:855184/1‑139 (MQ=255) cACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGa < 2:708091/95‑1 (MQ=255) cACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGa > 1:708091/1‑95 (MQ=255) tGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAac < 2:559393/223‑1 (MQ=255) tGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAac > 1:559393/1‑223 (MQ=255) cccGACCGGGCGAAGCGTGGCGGCGCAGGCGAACTTCTGGTTAGCCGTCCGCTGGACAGCG‑GCCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGc < 2:803500/288‑1 (MQ=255) ccgatcgagcgaagtatgggctcgaaggcgcaCTACGGGTTAGCCGTCCTATGTGCATCG‑ACCGGCCGGAGTTAATCGTCAAGGAGAC‑GCGTGTGGCGGTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCTGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGc < 2:399046/263‑1 (MQ=255) aCCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGa > 1:1150694/1‑289 (MQ=255) ccAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGaa < 1:1157421/289‑1 (MQ=255) ccAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGaa < 1:116419/289‑1 (MQ=255) ccAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCGGGACATCG‑ACCTGCCCCCGTTAATCGTCAAGGATAC‑GCGTCGGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGaa < 2:324666/289‑1 (MQ=255) cAGGCTGAAGCCGGCGGCGCAGGCGCACTACGGGTTGTCCGTCCGCTGGACATCG‑ACCTGCCGCAGGTAATCGGCAAGGATAC‑GCGTCTGGCTTTTGGTGAGCTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTGAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAAGCTCAACAACGACATCGGTGTACCGATTACGCTGTTGCGCTTAACGCCGGaa < 2:406570/288‑1 (MQ=255) aaaGGTGGCGGGGCAGGCGAACTACGGGTGAGCCGTCCGCTGGACATGG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGAt < 2:186349/288‑1 (MQ=255) tGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGc < 1:1026329/288‑1 (MQ=255) tGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGc < 2:738161/288‑1 (MQ=255) gcggcgggctggcgcaCTACTCGTTTGCCGTCCGCTGGACATCG‑ACTGGGCGCAGGTAAGCGTCAAGGATAC‑GCGCGTGGCGGTTGGTGGACTGGCTGAATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTGCGGCAAAACCTCCGGTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGc < 2:687464/278‑1 (MQ=255) gcggcgCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTCCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACAAGCTTTATACGGCAGGCAATCTCACCAACGACATCGGTGTACCGATGACGCGGTTGCGCTTAAAGCCGGAATACGATTACGCAGt > 1:161924/1‑289 (MQ=255) cgcaggcgcaCTACTGGTTTGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCGTGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTAt < 2:582836/288‑1 (MQ=255) ctactGGTTAGCCGTCCGCTGGACATCG‑ACCTGCGGCAGTTAATCGTCAAGGAGAC‑GCGTCTGGCGGTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCGCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATAACGGCGGCTATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATGTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGgcg < 1:1085790/289‑1 (MQ=255) gTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCATGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGAAGTTATTGAACTTGGCGCGAAcc > 1:256946/1‑289 (MQ=255) | CCATGAAGATTACCAGATTGTTGGCAATCAGCGTCTGGACTACTCCGATCGCGTCACGGTGGCGCGTCTGCTGGGGGTGATTGCATGATTAGCGTAACCCTTAGCCAACTTACCGACATTCTCAACGGTGAACTGCAAGGTGCAGATATCACCCTTGATGCTGTAACCACTGATACCCGAAAACTGACGCCGGGCTGCCTGTTTGTTGCCCTGAAAGGCGAACGT‑TTTGATGCCCACGATTTTGCCGACCAGGCGAAAGCTGGCGGCGCAGGCGCACTACTGGTTAGCCGTCCGCTGGACATCG‑ACCTGCCGCAGTTAATCGTCAAGGATAC‑GCGTCTGGCGTTTGGTGAACTGGCTGCATGGGTTCGCCAGCAAGTTCCGGCGCGCGTGGTTGCTCTGACGGGGTCCTCCGGCAAAACCTCCGTTAAAGAGATGACGGCGGCGATTTTAAGCCAGTGCGGCAACACGCTTTATACGGCAGGCAATCTCAACAACGACATCGGTGTACCGATGACGCTGTTGCGCTTAACGCCGGAATACGATTACGCAGTTATTGAACTTGGCGCGAACC > NC_000913/94566‑95136 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |