Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,708,805	T→C	21.4%	R629R (CGT→CGC)	rsxC →	SoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,708,805	0	T	C	21.4%	87.5 / 3.8	48	R629R (CGT→CGC)	rsxC	SoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base T (15/20);  new base C (7/2);  total (26/22)
Fisher's exact test for biased strand distribution p-value = 1.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCA‑AAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAG‑AACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGC  >  NC_000913/1708517‑1709093                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                  gcAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCA‑AAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCCAATGCGGAACCAGAAGAACAGGTCGCTCCGGGCACAGCCTCCGCAGAAGCCGCGAACGCCCGt                                                                                                                                                                                                                                                                                                   >  1:924149/1‑289 (MQ=21)       cAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGAAGCAGCAACCTTTGATGATCCACGGAAAACTGCCGTTGAAGCAGCTATCGCCAGCGCCA‑AAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCAGAACCAGAACAACAGGACGATCCGCGCAAAGCCGCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCaaa                                                                                                                                                                                                                                                                                             <  1:250314/289‑1 (MQ=255)          agCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCA‑AAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCCAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTTGATCCGAGCAAAGCCGCCGTCGCCGCAGCTATTGCCGGTGCCAACGc                                                                                                                                                                                                                                                                                           >  1:491850/1‑288 (MQ=255)                            tcgtataagagacagcgCAGCAACCGTTGCTGATCCACGTAAAACTGCCCTTGAAGCAGCTATCGCCCGCGCCA‑AAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGACAAAGCACGCAAGCTGGAACAGCaa                                                                                                                                                                                                                                                                        <  1:1140385/274‑1 (MQ=255)                             cagcaAACTAACGAGGCAGCAAACGTTGCTGTTCCACGAAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCA‑AAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCaa                                                                                                                                                                                                                                                                        <  1:669938/288‑1 (MQ=255)                             cagcaAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCA‑AAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCAGACGAAGCCGCCAGTGCCCGTGCCAAACCGCGCAAAGCGGAGCGGCCACCGGCTAATGCCGAACAAGAAGAACAGGTCGATACGCGGAAAGCCGCCGTCACAGGCGCCAGTGCCCGGGCAAAAGCCCGGAACCTGGGAGaacaac                                                                                                                                                                                                                                                                       >  2:1139511/1‑289 (MQ=17)                                       aagacgCGGAAACCGTTGCTGTAACAGGGAAAATGGCAGTTCAGGCAGCTCT‑GGCCGAGCCAGAAGGGTCCAAGCTGGAAAAGCAACAGGCTAATGCCGAACCAGAACAACAGGTTGATCCGCGCAAAGTCTCCGTCTAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGGCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGc                                                                                                                                                                                                                                                             <  2:1193469/287‑1 (MQ=14)                                               acaACCGATGCTGATGCACGAAAAAATGCCGTGGAAGCAGCTATCGCCCGCGCCA‑AAACGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTGGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATCGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAg                                                                                                                                                                                                                                                     <  1:1018202/288‑1 (MQ=255)                                                       tGCTGATCCACGTAAAAGTGCGTGTGAAGCAGCCATCACCCGCGCCA‑AAGCGCGCAAGCTGGAACAGTAACAGGCTAAAGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAG‑aaca                                                                                                                                                                                                                                                <  1:124299/285‑1 (MQ=255)                                                            aTCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCA‑AAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCACGCAACCCGGAACAGCAACAGGCTATTGCGGCGCCCGAAGAACTGGTCGATCCGCGCAAAGCCGACGTCGAAGACGCTATTGCCAGAGCCAAAGCACGCAAGCGGGAACAGCAAACGGCTAATGCGGACGACG‑AACAACAggcaca                                                                                                                                                                                                                                       >  2:999329/1‑285 (MQ=14)                                                                 cGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCA‑AAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCCCCAAAGCACGCAAGCTGGAACCGCAACAGGCTAATGCCGAGACAC‑AAAAACAGGTCGAAcccg                                                                                                                                                                                                                                  >  1:633106/1‑287 (MQ=255)                                                                                                 cgcCA‑AAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCCCGCGCCAAAGCACGCAAACTGGCACAGCAACAGGCTAATGCCGAGCCAG‑AACAACAGGTCGAACCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCg                                                                                                                                                                                                   >  1:186831/1‑288 (MQ=255)                                                                                                 cgcCA‑AAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAACCGGAACAGCAACAGGCTAATGCCGAGCCAGAAGAACAGGTCGATCTCCGCAAAGCCGCCGTCGACGCCGCTATTGCCCGAGCCACAGCACGCAACCGGGCACAGCAACAGGCTAATGCCGAGCAAG‑AAGAACAGGTCGATACGCGCAAAGCAGCCATCCACGCCGCTATTGACCGa                                                                                                                                                                                                  >  2:372815/1‑289 (MQ=11)                                                                                                            cgcAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCCAGCAAGCTGGAACAGCAACAGGCTAACGCCGAGCCAG‑AACCACAGGTCGATCAGCGCAAAGCCGCCGGTGAAGCCGCTATTGCCCGAGCCCAAgccc                                                                                                                                                                                        >  1:872288/1‑287 (MQ=14)                                                                                                            cgcAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCACCCAAACCGCAACAGCAACCGGCTACTGCAGACCCAGAAAAAACAGG‑CGGTGCGCGCAACGCCGCAGTAGAAGCCgacgtacacccagcccaccgc                                                                                                                                                                                         >  2:814162/1‑268 (MQ=11)                                                                                                                 gCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCAGTGGCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGCGCCAA‑CACAACAGGGCGCACGCCGCAAAGCAGCAGCCAAAACCGCGATTGCTCGTGCCAAAgaacacaa                                                                                                                                                                                    >  1:89472/1‑281 (MQ=14)                                                                                                                           cAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCCCGCAAGCTGGAACAGACACAGGCTAATGCCGAGCCAG‑AAGAACAGGTCAATCCTCGCAAAGCCGCCGTCAAAGCTGCGATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGc                                                                                                                                                                         >  1:189502/1‑289 (MQ=17)                                                                                                                             aCAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCCATTGCCCGTGCCAAAGCACGCAAGCCGGAACAGCAACAGGCTAATGCCGAGCCCG‑AACAACAGGTCGATCCGCGCAAAGCCGCCGTCGCAGCCGCTATTGCCCGAGCCACAGCGCGCAAACGCGACCAGCa                                                                                                                                                                        >  1:747102/1‑288 (MQ=255)                                                                                                                                     aTGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGACGCGGCTATTCCCCGCGCCAAAGCCCGCAAACTGGAACAGCAACAGGCTAATGCCGAGCCAG‑AACAACAGGTCCGTCCGCGCAACGCCCCCGTCGCCCCCGCCATTGCCCCGCCCCAGGCCCGAAAACCGGACCAGCCACAGGCttc                                                                                                                                                               >  2:1087855/1‑287 (MQ=14)                                                                                                                                           aaCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCCCGCGCCAAAGCGCGCAAGCCGGAACAGCAACAGGCTACTGCGGAACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCCGTCGCAGCCGCTATTGCCCGCGCCCAAGCACGCTACACAAAACCGCAACTGGCTACGGCCCACCCAG‑CATAAAAGGTGGATACGCCCAAAGCAGCCGTAGAAGACGCTCATGCCCGAGACCCAGCACACAAGCACGGACAGCAACCGGCGAAAGCAGa                                                                                                                                                         >  2:319265/1‑289 (MQ=9)                                                                                                                                           aaCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTCGATCCTCGCAAAGCCGCCGTCGAAGCCGCAATTGCCCGTCCCAAAGCACCCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAG‑AACAACAGGTCCATCCCAGCAAACCCGCCGTCCACACCGCTCTCACTCGACCCAAAGCGCGCAAACGGGCCCAGCAACCgtacactaccga                                                                                                                                                         >  2:503360/1‑278 (MQ=17)                                                                                                                                            aCCAGAACACCGGGTCGACCCGCGCAAAGCCGCCGTCGAAGCCGCCAGGGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAG‑AACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGa                                                                                                                                                         <  1:58293/288‑1 (MQ=255)                                                                                                                                                  acaacaGGTCGATCCGCGCAAAGCCGCCTTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAG‑AACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCag                                                                                                                                                    <  1:503360/287‑1 (MQ=255)                                                                                                                                                    aaaaGGTCGATCCGGGCAAAGCCGCCGTCGAATCTGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTGGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACGGCAACAGGCTAATGCCGAGCCAG‑AACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCagaaga                                                                                                                                                <  1:254408/286‑1 (MQ=255)                                                                                                                                                      caGGTCGTTCCGCGAAAAGCCGCCGTCGAAGCCGCTATTGCCCGCCCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCTGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAG‑AACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAAc                                                                                                                                              <  1:1139511/289‑1 (MQ=255)                                                                                                                                                       ttgctgctCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCGGCCGTCGAAGCCGCTATTGCCCGCGCGAAAGCCCGCAAGCTGGGACAGCAAAAGGCTAAGGCGGAACAAG‑AAGAACACTTGGATCCTCGCAAAGCAGCCGTTGACGCTGCCAATGCCCGAGCCCAAGCCAGGCAACGCGAACATCCACCGGCTaaaaacacgccagaaaaaca                                                                                                                                             >  2:225690/8‑271 (MQ=14)                                                                                                                                                        ggTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAG‑AAGAACAGGTCGATCCGCCCAAAGCCGCCGTTGACGCGGCCCTTGCCCGCACCCAAGCCCGAAAGAGCGCACAGCACCAGGCTGAAGCGGCGGCacaagcagca                                                                                                                                            >  2:1183067/1‑284 (MQ=14)                                                                                                                                                                  cgcAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCACCTGCCAAAGCACCCCAACTGGCACAGCAACCGGCCAATGCCGAGCCAG‑AATCACCGGTCGATCCGCGCAAACCCGCCGTCGAAGCCGATATTGACACTGCCACAGCTCGCCCACGGAAACCACAACCGGCCACTCACGAGCCAGACACACAGGtctatctga                                                                                                                                  >  2:236433/1‑281 (MQ=14)                                                                                                                                                                              gTCGAAGCCGCGATTGCGCGGGCCAAAGCGCGCAAGATGAGAAAGCAACAGGCTAATGCGGAGCAAGAAGAACAGGACGATCGGCGCAAAGACGCCGGCGAAGCCGCTATTGCCCGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAG‑AACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCg                                                                                                                      <  1:688100/289‑1 (MQ=17)                                                                                                                                                                                      cgcgATTGCCCGGGCGAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAG‑AACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAg                                                                                                                <  1:642308/287‑1 (MQ=255)                                                                                                                                                                                      cGCCATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACAAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAG‑AACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGc                                                                                                               <  1:236433/288‑1 (MQ=255)                                                                                                                                                                                          caagaccGAGCCACAGCACTCAAGCTGAAAGAGCAACAGACTCATCAGGAACCAGGAAAACAGGCAGGTCCCCGAAAAGCGGCCGACGAAGCAGCTATTGCCCGCGACAAAGCACGCAATCTGGAACAGCAACAGCCTAATGCCGAGCCAG‑AACAACAGGTCGAGCCGCGCAAAGCCGCCGTCGAAGCCGCTATGGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTa                                                                                                          <  2:1111943/284‑1 (MQ=255)                                                                                                                                                                                              cccGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGCACAG‑‑CGAGCCCCGCACAGCCGCCCCCGACGGACCCATTTCACGAGCCACAGCGCGCAAACTTGAGAAGCAACCGGCCAATTTCGGGCCAG‑AACAAAAGGGGGACACTCGCAAACACACCACCCAACCCGCCATTGCGCGCCCCACAGGCCCCAAACGGGTACGGGAACCGGTCAAAACACGCACAGAAAAACAAGTTTATCCCCGGAAAGCTGCCATCAAAgacactcagagat                                                                                                    >  2:760725/1‑277 (MQ=255)                                                                                                                                                                                                 gTGCCAAAGCGAACAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAG‑AACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCAcg                                                                                                   <  1:999329/289‑1 (MQ=255)                                                                                                                                                                                                   gCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAG‑AACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCCcgc                                                                                                  >  1:845722/1‑288 (MQ=255)                                                                                                                                                                                                             gcAAGCCGCAACAGCAACAGGCTAATGCGGAACCATAAGAACAGGTCGATCGGCGAAAAGCCGCCGTCGAAGACGCTATTGACCGTGCCAAAGCACGCAAGCTGAAACAGCAACAGGCTAATGCCGAGCCAG‑AACAACAGGTCGATCCGCGCAAAGCTGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACg                                                                                       <  1:809487/289‑1 (MQ=255)                                                                                                                                                                                                                           cAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCAATTGCGCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAG‑AACAACAGGTCGATCCGCGCACCGACGCCCAACAAGCCGCCCTTGCCCACGACCAAGTCCGCAAACCGGAACAGCACCCGGCCTATGCGGAACCAGAAGCACAGGTCGATCCCCCCATACTCACCGTCCAACCGGCTATTGCACGCGCCaaaacgacaacgacggctcagc                                                                         >  2:38935/1‑270 (MQ=9)                                                                                                                                                                                                                             accggctaagtgggAACCAGACGAACGGGTCGATCCGCGCAACGCCGCGAGCGAAGCCGCGATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAG‑AACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCaa                                                                       <  1:883847/277‑1 (MQ=17)                                                                                                                                                                                                                                                    aacgaggccaTCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGGCCGTGGAAAAGCACGCAAGCTGGAACAGCAACAGGCTAATTCCGAGCCAG‑AACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATGGCCCGAGCCAAAGCGCGCAAGCGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCagaaga                                                <  1:38935/280‑1 (MQ=255)                                                                                                                                                                                                                                                     aCAGGGCGATCCGCGCAAAGCCGCCGGCGAAGACGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAG‑AACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCagaaga                                                <  1:760725/288‑1 (MQ=255)                                                                                                                                                                                                                                                       aGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAG‑AACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAGCAGCAACAGGCTAATGCGGTACCAGAAGAACa                                             >  1:248739/1‑289 (MQ=255)                                                                                                                                                                                                                                                         gTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAG‑AACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCACGCAGAGCTGCCGTCGCACACGCTATCGGCCGAGCCAAAGCCCGCAAGCTGGCACAGCAACACGCTACTACCGCAGCAgaagaagcgg                                           >  2:580555/1‑285 (MQ=14)                                                                                                                                                                                                                                                             aTCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAG‑AACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCCAGAAAGCTGGAACAGCAACAGGCTCATGCGGTACCAGAAGAACAGGTTGa                                       >  1:1120393/1‑289 (MQ=255)                                                                                                                                                                                                                                                              tCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAG‑AACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCaa                                                                                    >  2:1125897/1‑243 (MQ=255)                                                                                                                                                                                                                                                              tCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAG‑AACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCaa                                                                                    <  1:1125897/243‑1 (MQ=255)                                                                                                                                                                                                                                                                                   aGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAG‑AACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCAGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCGATTGCACGCGCCAAAGCACGAAAGCTGGAACAGCAACAGGCTAATGAGGTACAAGAAGAACAGGTTGAACCGCGCAAAACGGCAATTGc                  >  1:821903/1‑288 (MQ=255)                                                                                                                                                                                                                                                                                         tATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAG‑AACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACCGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCt           >  1:706700/1‑289 (MQ=255)                                                                                                                                                                                                                                                                                           ttGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAG‑AACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTAt         <  1:814162/289‑1 (MQ=255)                                                                                                                                                                                                                                                                                           ttGCCAGTGCCAAAACACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAG‑ACCAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAGCGGGAACGGCAACCGGCTAATGCGGAACCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTAt         <  1:319265/289‑1 (MQ=255)                                                                                                                                                                                                                                                                                                  tGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAG‑AACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGACCAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGACGCGGCTATTGCCCGCGCCAAAGCACGCAAGCTGGCACAGCCACAGGCGAATGCGGTACCAGAAGAACAGGTTGAACCGCCCAAAGCGGCAGGTGCCGCGGCCATTCCCcg   >  2:878172/1‑288 (MQ=17)                                                                                                                                                                                                                                                                                                  tGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAG‑AACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCTGTCGCCGCGGCTATTGCCAGCGCCAAAGCACCCAAGCAGGAACAGCAACAAGCTAATGCGGTACCCGAAAACAAGCTTAAAACGCCACACGCGGCAGTTGCCGCGGCTAttctccc   >  2:411192/1‑283 (MQ=17)                                                                                                                                                                                                                                                                                                  tGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAG‑AACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAgaagaa                                               >  1:35782/1‑244 (MQ=255)                                                                                                                                                                                                                                                                                                  tGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAG‑AACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCcgc  >  1:817045/1‑289 (MQ=255)                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                  GCAAGCCAGAGCGAAACAGGCAGAACTGCAGCAAACTAACGACGCAGCAACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCA‑AAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAG‑AACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGC  >  NC_000913/1708517‑1709093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑