Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,304,538	C→T	5.3%	intergenic (+147/+568)	eco → / ← mqo	ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,304,538	0	C	T	5.3%	255.3 / 4.9	97	intergenic (+147/+568)	eco/mqo	ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base C (51/38);  new base T (2/3);  total (55/42)
Fisher's exact test for biased strand distribution p-value = 6.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.50e-01

CACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTAT‑ACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGC  >  NC_000913/2304253‑2304825                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                 caccgggttgatgtgggATGCTCGGAGGCGGCGTTACAACATCAAGCTGCCGATCG‑GGAGTTTGACACCAGACAATGGATACGGGAAGCACCGCACATGGAAGGGGGAAGAAAAAAC‑TGACAACGC‑GGTAGTTGTGTCAACTGCCATGAAGTGCGGCACACCGTAGGTCAGACAAAGCGGTCACGCCTCATCCTACATACAACGCCCGAGCCTGTTGCCTGATGCGACGCTGACGCGTTTTAGCAGGCCTACACGGCTGTGAAGTGCGGCACCCCGTAGGTCAGACaa                                                                                                                                                                                                                                                                                                <  2:43256/272‑1 (MQ=255)              ggCGATGTTGGAATGCTGCGTGACAACAGCAAGCTGCCGATCGTGGTGTAT‑ACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCGACACCGCTGTGCAGTTCGGCACCCCGTAGGTCAGACAAGGCCG‑TCAcgccgc                                                                                                                                                                                                                                                                                 >  1:7717/1‑289 (MQ=255)               catgtgatgaaaTGCTGCGGTACACAGGCAAGCTGCGGATCGTGGTGTAA‑ACGCCAGACAAGGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACCGCCGTGAAGGGCAGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCAcgccg                                                                                                                                                                                                                                                                                  <  2:678291/277‑1 (MQ=21)                 gATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTAT‑ACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCAt                                                                                                                                                                                                                                                                               >  1:1106995/1‑288 (MQ=255)                   tGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTAT‑ACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAACTTCCGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCg                                                                                                                                                                                                                                                                            >  1:1094489/1‑289 (MQ=255)                    gCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTAT‑ACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGa                                                                                                                                                                                                                                                                           <  1:340967/289‑1 (MQ=255)                             ctgcGTTACAACAGCAAGCTGCCGATCGTGGTGTAT‑ACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCGGACAAGGCGG‑CCACGCAGCAACCGACACCCAAc                                                                                                                                                                                                                                                                   >  1:329494/1‑288 (MQ=255)                             ctgcGTTACAACAGCAAGCTGCCGATCGTGGTGTAT‑ACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGCCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCTTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGTACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAAc                                                                                                                                                                                                                                                                   >  1:219333/1‑288 (MQ=255)                             ctgcGTTACAACAGCAAGCTGCCGATCGTGGTGTAT‑ACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCAGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACAt                                                                                                                                                                                                                                                                        >  1:678291/1‑283 (MQ=255)                                cGTTACAACAGCAAGCTGCCGATCGTGGTGTAT‑ACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGccc                                                                                                                                                                                                                                                               >  1:145387/1‑289 (MQ=255)                                 gTTACAACAGCAAGCTGCCGATCGTGGTGTAT‑ACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGG‑TTACGCCGCATCCGACATCTAACGccca                                                                                                                                                                                                                                                              <  1:516450/289‑2 (MQ=255)                                          gCAAGCTGCCGATCGTGGTGTAT‑ACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGACGCGACGCTCGCGCGTCTTATCAGGCCTACACCGCTGCGAAGTGCGTCACCCCGTAGGCAAGTCCAGGCGGTTCAC‑CCACAACCGACCGCCAACGCCCGTACCGGtt                                                                                                                                                                                                                                                      >  2:639548/1‑288 (MQ=25)                                             aGCTGCCGATCGTGGTGTAT‑ACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGCGCCGGTTGCCTGATGCGACGCTGGCGCCTCTTATCAGGCCTACACCGCTGTGAAGTGCAGCCCTCCGCAGGCCTGCGAAGCCGG‑TCACGCCGCACCCGACCTCCAAAGGCCCAACCGGTTAcc                                                                                                                                                                                                                                                   >  2:964974/1‑288 (MQ=21)                                                  ccGATCGTGGTGTAT‑ACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGc                                                                                                                                                                                                                                             >  2:760803/1‑289 (MQ=255)                                                            tgtAT‑ACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGACTGATGCGAAGCCGgc                                                                                                                                                                                                                                    >  1:394251/1‑288 (MQ=255)                                                                t‑aCGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTTAAGTGCTGCAACCCGTAGGTCAGACAAGGCGG‑TCATGCCGAATCCGACATACACCGCCCACGCAGGTTGCCGGCTGCgccctcggcgccg                                                                                                                                                                                                                                >  2:353330/1‑276 (MQ=18)                                                                t‑aCGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCCTCCGACATCCAACGCCCGAGCCGCTTGCCTGATGCGACGCTGGcgcg                                                                                                                                                                                                                                 >  2:416805/1‑287 (MQ=255)                                                                    gCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTa                                                                                                                                                                                                                            >  1:133893/1‑289 (MQ=255)                                                                     ccgagaaagggggATGTGAAGTACGGCGTCTGGAGGACGGAAGAGAAAAT‑TGAAAGCGC‑GGTAGTTCGCGAAACTGCCGTGAAGGGCGGCACCCCGGAGGGCAGACACGGCGGTCACGCAGCATCCGACACCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                                                                                                                                                           <  2:882637/277‑1 (MQ=18)                                                                     aaagaaaaatgagatgggcagatgcgcgGCTGGAAGGCGGAAGTGCGAAT‑GGACACCGG‑GACAGTCCGCTAAACGGCCGGGAAAGGCGGCACCCCGTAGGTGAGACGGGGCGGGCACGCCGCATCCGACATCCAACGCCCGGGCGGGTGGCCTGATGCGACGCTGGAGCGTCTTATCAGCCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCGGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                                                                                                                                                           <  2:484183/265‑1 (MQ=14)                                                                      cAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                                                                                                                                                           >  1:538624/1‑288 (MQ=255)                                                                      cAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATc                                                                                                                                                                                                                          <  1:280793/289‑1 (MQ=255)                                                                      cAGACAATGAAGATGGGCAGTACCGCGGCGGGAAGGCGGAAAAGAAAAT‑TGACAACGG‑GGGAGTTGGCGAAACGGCCGTGAAATGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGAGGCGACGCTGGCGCGTCTGATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                                                                                                                                                           <  2:1106995/288‑1 (MQ=17)                                                                         aCAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGG‑TTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGCTGCGACGCTGGCCCGTCTTATCAg                                                                                                                                                                                                                        >  2:51298/1‑288 (MQ=255)                                                                         aCAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCTAAc                                                                                                                                                                                                                                                                   >  1:853440/1‑245 (MQ=255)                                                                         aCAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                                                                                                                                                           >  1:636679/1‑285 (MQ=255)                                                                         aCAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAg                                                                                                                                                                                                                        >  1:999987/1‑288 (MQ=255)                                                                         aCAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAg                                                                                                                                                                                                                        >  1:401590/1‑288 (MQ=255)                                                                         aCAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGAAAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGATGGCGCGTCTTATCAg                                                                                                                                                                                                                        >  1:1090255/1‑288 (MQ=255)                                                                         aCAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGACCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCATGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTGTCAg                                                                                                                                                                                                                        >  1:687407/1‑288 (MQ=255)                                                                             tGTAGATGCGAAGTACCGTGTCTGGAAGGCGGAAGAGAAAAT‑TGAAAACGC‑GGTAGTTCGCTAGAGTGCCGTGAAGTGCGGCACCCCGTAGGGCAGACAATGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGGCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTa                                                                                                                                                                                                                   <  1:134532/289‑1 (MQ=255)                                                                               aggacaggAAGTACAGGGTCACGAGGGCGGAAGGGAAAAT‑TGTCAATAC‑GGTAGGTCGCAGAACTGCCGTGGAGTGCAGCACCCCGTAGGATAGACAAGGCGGTCACGCCGCAGCCGCAATCCGACGCCCGGGCCGGTTGCCTAATGCGACACTGGCACGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTaca                                                                                                                                                                                                                 <  2:292751/282‑1 (MQ=17)                                                                                  aTGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCATACCAGGCAG‑CCACGCCGCATCCGACATCCAACCCCCGCGCCGGTTACCCGATGCGACGCCGGCCCGTCTTAACAGGCCCACAcc                                                                                                                                                                                                               >  2:19511/1‑288 (MQ=18)                                                                                   ggggaagttgagcgTCTGGAAGGGAAAAGAGAGAATGTGA‑AACTC‑GGTAGTTCGTTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCGGATAAGGCGGTTGCGCCGCATCCGACATCTATCGGCGAAGCCGGTTGCCTGATGGTACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGG‑TTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGCTGCGACGCTGGCGCGTCTTATCAgcatacgccac                                                                                                                                                                                                              <  2:845242/277‑11 (MQ=11)                                                                                     tgAAGTACCGCGTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAcc                                                                                                                                                                                                               >  1:495158/1‑285 (MQ=255)                                                                                     tgAAGTACCGCGTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAcc                                                                                                                                                                                                               >  1:1105511/1‑285 (MQ=255)                                                                                       aaGTACCGCGTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACCCCGCtgtg                                                                                                                                                                                                         >  1:503534/1‑289 (MQ=255)                                                                                       aaGTACCGCGTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCtgtg                                                                                                                                                                                                         >  1:992247/1‑289 (MQ=255)                                                                                       aaGTACCGCGTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCtgtg                                                                                                                                                                                                         >  1:109736/1‑289 (MQ=255)                                                                                       aaGTACCGCGTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCACCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCAATCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCATGCCTACACCGCtgtg                                                                                                                                                                                                         >  1:781391/1‑289 (MQ=255)                                                                                        aatgtgggggaaggAAGGCGGAAAAGAAAA‑‑TGCGAACGCGGGGAAGGCGCGAAACAGCCGTGACGTGCGGAACCCGGTCGGTCAGACAAGGCGGGCAGGCCGCATCCGTCTGCCAACGCCCGAGCCGGTTGCCTGATGGAACGCTGGCGCGGCTTATCAGGGCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCtgtg                                                                                                                                                                                                         <  2:576213/276‑1 (MQ=14)                                                                                           aCCGCGTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGG‑TTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGaa                                                                                                                                                                                                       <  1:51298/287‑1 (MQ=255)                                                                                            ccGCGTCGGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTg                                                                                                                                                                                                    <  1:1100619/289‑1 (MQ=255)                                                                                              gcgTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCt                                                                                                                                                                                                  <  1:416805/289‑1 (MQ=255)                                                                                               cgTCTGGACGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTCCACCGCTGTGAAGTGCGGCACCCTGTCGGTCCGACAAGGCGG‑TTACGCCGAATTCGACATCCAACGTACCAGCCGGTGGCGTGATGAGACCCTGGCGCTCCTTATctgtcccacccgcccgctcactgct                                                                                                                                                                                                  >  2:1181450/1‑264 (MQ=17)                                                                                               cgTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGGAGTGCt                                                                                                                                                                                                  >  1:643405/1‑288 (MQ=255)                                                                                               cgTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCt                                                                                                                                                                                                  >  1:482379/1‑288 (MQ=255)                                                                                               cgTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCt                                                                                                                                                                                                  >  1:915485/1‑288 (MQ=255)                                                                                               cgTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑ACACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGGCTTATCAGGCCTaccactgccactcgaccac                                                                                                                                                                                                 >  1:1017336/1‑272 (MQ=255)                                                                                                 tCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGG‑TTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTcc                                                                                                                                                                                                >  1:483318/1‑288 (MQ=255)                                                                                                 tCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGCGaa                                                                                                                                                                                                       >  1:292751/1‑281 (MQ=255)                                                                                                  cTGGAAGGGGGAAGAGAAAAT‑TGCCAACGC‑GGTAGTCCGCTAACCTGCCGTGAAGCGCGGCACCCCGTAGGGCAGACGAGGCGGGCACGCCGCATCCGACAGCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGGGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCa                                                                                                                                                                                               <  2:482379/288‑1 (MQ=17)                                                                                                  cTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAACTGCGGCACCCCGTAGGGCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAATGCTCCa                                                                                                                                                                                               <  1:19511/288‑1 (MQ=255)                                                                                                           ggAAGGGAAAAT‑GGAGACCGC‑GGTAAGTGCCTAACAGGCCGGGAAGTGCGGCACCCGGTAGGTAAGAGAAGGCGAGCCCGCAGCATCGGAGTGCAAACGCCCGAGCGGGTGGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGGGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAgg                                                                                                                                                                                      <  2:495158/288‑1 (MQ=14)                                                                                                           ggAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACACGGCGG‑TTACGCCGCATCCGACATCCAACGCCCGAGCCGGTTTCCTGATGCGACGCTCTCGCGCCTCATCAGGCCTAAAcccccgcgaacttcctcacccagcaggt                                                                                                                                                                                     >  2:5358/1‑263 (MQ=17)                                                                                                                    aaT‑GGACAACGC‑GGGAGGTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGTCATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAgg                                                                                                                                                                            <  1:646308/289‑1 (MQ=255)                                                                                                                          aCAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGtt                                                                                                                                                                       >  1:287275/1‑289 (MQ=255)                                                                                                                                        tCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGa                                                                                                                                                          <  1:760803/289‑1 (MQ=255)                                                                                                                                                      tGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGCCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGACGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTAGCCCGATGCGCCGATGGCGCGTCTTATCAGACCGACACCGCTGTGACGTGCGCCACCCCGTAGGTCGGATAAGGCGGTTACGACGCAACCGAAATCTAACGCCaaa                                                                                                                                            >  1:1077577/1‑289 (MQ=11)                                                                                                                                                         agggCGGCACCCCTGAGGGCAGGCAAGGCGGTCACGCCGCAGCCGACAGCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAGCAGGCCTACACCGCTGTGAAGGGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGc                                                                                                                                          <  1:569341/285‑1 (MQ=255)                                                                                                                                                                 aCCCCGTAGGTCAGACAAGGCGGTCACGCCGCAGCCGACATCCAACGCCCGGGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCt                                                                                                                                 <  1:919852/289‑1 (MQ=255)                                                                                                                                                                     cGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCg                                                                                                                                                                                                              >  2:527806/1‑208 (MQ=255)                                                                                                                                                                     cGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCg                                                                                                                                                                                                              <  1:527806/208‑1 (MQ=255)                                                                                                                                                                                    gCGGTCACGACGCATCCGACATCAAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCGGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGt                                                                                                               <  1:52456/288‑1 (MQ=255)                                                                                                                                                                                         cACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                                          <  1:736298/288‑1 (MQ=40)                                                                                                                                                                                         acagccgattcgagacgccAACGCCCGGGCCGGTTGCCGGGTGGAACACTGGGCCGCCTAAGCAGGCCTAGACCGCTGGGAAGTGCGGCACCCCGTAGGTCAGACAGGCCGG‑TCAAGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCATCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGAGAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                                          <  2:503534/271‑1 (MQ=14)                                                                                                                                                                                                catcggcagtCCACCGCGCGGGCCGGTGGCCTAAGGCGCAGCTGGCGCGTCTGATCAGGCCTACACCGCGGTGAAGGGCGGCCCCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGGGCCGGTTGCCTGAGGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTa                                                                                                  <  2:109736/280‑1 (MQ=14)                                                                                                                                                                                                  tCCGACATCCAGCCCCCGACGCGTTGGCCGAGAGGGAGGCTGGCGCGTCTTACGAGGCCTACACCGGCTTGAGGTGCCGCACCCCGTAGGTCAGACAAGGCGT‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTaca                                                                                                <  2:999987/289‑1 (MQ=11)                                                                                                                                                                                                     tgccgcacttcacagcggtgtaggCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAcc                                                                                              >  1:934055/24‑288 (MQ=255)                                                                                                                                                                                                                 cggtgaggggggcgtGATGGGAGGCGGGCGCGGCTGATCAGGGCTACACCGCTGGGAAGGGCGGCACCCCGTAGGTCAGACAGGGCGG‑GCACGCCGCATCCGACATCCAGCGCCCGAGCCGGTTGCCGGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCt                                                                                 <  2:401590/275‑1 (MQ=11)                                                                                                                                                                                                                    aGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCg                                                                                                                                                                                          <  1:1168926/181‑1 (MQ=255)                                                                                                                                                                                                                    aGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCg                                                                                                                                                                                          >  2:1168926/1‑181 (MQ=255)                                                                                                                                                                                                                     gCCGGTTGCCGGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCa                                                                              <  1:639548/288‑1 (MQ=38)                                                                                                                                                                                                                              ctgagaggacagtgaggcgTCATGTTAGGCCTACCCCGCTGTGAAGTGCGGAACCCCGTGGGTCAGACAAGGCGG‑TCGAGCCGCATACAACATCCAACGCCCGAGCCGGTTGCCTGAGGCGACGCTGGCGCGTCTTATCAGGCCTAAACCGCTGTGAAGTGCGCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAgg                                                                     <  2:915485/272‑1 (MQ=11)                                                                                                                                                                                                                              cTGAGGCGACGCTGTCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCAAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAgg                                                                     <  1:353330/288‑1 (MQ=255)                                                                                                                                                                                                                                       cGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATaa                                                                                                                                                                              <  1:73748/174‑1 (MQ=255)                                                                                                                                                                                                                                       cGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATaa                                                                                                                                                                              >  2:73748/1‑174 (MQ=255)                                                                                                                                                                                                                                       cGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAgg                                                           >  1:773559/1‑289 (MQ=38)                                                                                                                                                                                                                                             cgcgTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATc                                                                                                                                                             <  1:1094946/185‑1 (MQ=255)                                                                                                                                                                                                                                             cgcgTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATc                                                                                                                                                             >  2:1094946/1‑185 (MQ=255)                                                                                                                                                                                                                                               tcagagacgggttcTAGACCGCTGTGTAGTGCGCCACCTCGTAGTGAAGACAAGGCGG‑CCTGGTCACATGCGACTGCCACCGTCGGCGGTGCTGGGCGGATGCGACGCTGTCGCGTCTTATCCGGCCTACACCTCTGTGAAAGGCTGCACCCCGTAGGTCGTATAAGGCGGTTTCGCCGCATCCGACATCTAACGCGCGAGCAGGTTGGCTGATGCGACGCTTGTGCGGCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTAcg                                                   <  2:765823/276‑1 (MQ=2)                                                                                                                                                                                                                                               ccgcggtgttgcgcTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTGGGCCATGGGGG‑TCACGCCGCATCCGACATGCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCAGCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTAcg                                                   <  1:469094/276‑1 (MQ=11)                                                                                                                                                                                                                                                           ccTACCCCGCTTTGAGGTGCGGCACCCCTTAGGTCAGGAAAGGCGG‑TGACGCCGCATCCGATATCCAACGCCCGCGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCAcccc                                                                          <  1:927294/254‑1 (MQ=11)                                                                                                                                                                                                                                                                    cTGTGAAGTGCGGCACCCCGTAGGTCAGTCAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGt                               >  1:370312/1‑288 (MQ=255)                                                                                                                                                                                                                                                                    cTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCCGGCCTACACCGCTGTGAAGTGCTCCACCCCCTAGGTTGGATAAGGCGGTTTCGCCGCATCCGACATccccagc                               >  1:1111934/1‑283 (MQ=255)                                                                                                                                                                                                                                                                    cTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTaca                                                                                                >  1:16130/1‑223 (MQ=40)                                                                                                                                                                                                                                                                    cTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCAACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGt                               >  1:23464/1‑288 (MQ=255)                                                                                                                                                                                                                                                                    cTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGCCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGACGGATAAGGCGGTTACGCCGCATCCGCCATCTAACGCCCAAGCCGGTTGTCTGCTGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGGAGTGCTCCAACCCGTAGGTTGGATAAGGCGGTTACGCCGCAACCGa                                         >  1:765823/1‑278 (MQ=255)                                                                                                                                                                                                                                                                    cTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATACAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGt                               >  1:821900/1‑288 (MQ=255)                                                                                                                                                                                                                                                                          agggccgccccccgtggggcgagcAAGCCGG‑TCACGCCGCATCGTACATCCGACGCACGAGCCGGTGGCCTGAGGGAACACTGGGACGTCTTATCAGGCCTACACCGCTGTGAAGTGCTGCACCCCGTAGGTCGGATAAGTCGGTTACGCCGCATCCGACATCAAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGc                         <  2:538624/265‑1 (MQ=11)                                                                                                                                                                                                                                                                              cGGCCCCCCGTGGGTCAGACAAGGCGG‑GCACGCCGCATCCGACATCCAACGCCCGGGCCGGTTGCCTGATGCGACGCTGGCGGGTCTTATCAGGCCTACAAAGCTGTGAAGTGCTCCACACCGTAGGTCGGATAAGGGGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGt                     <  1:1181450/288‑1 (MQ=255)                                                                                                                                                                                                                                                                              cGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAgg                                                                                                                                                                                      <  1:25384/127‑1 (MQ=35)                                                                                                                                                                                                                                                                              cGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAgg                                                                                                                                                                                      >  2:25384/1‑127 (MQ=35)                                                                                                                                                                                                                                                                                 cACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATcc                                    <  1:392766/270‑1 (MQ=38)                                                                                                                                                                                                                                                                                   ccccGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCGGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTg               >  1:974943/1‑289 (MQ=39)                                                                                                                                                                                                                                                                                      cctAGGTCAGACACGCCGG‑CCAGGCCCCATCCCACAGCCAGCGCCCGGCCCGGTTGCCGAATGCGACCCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCGAGCCGGTTGCCTGATGCTACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATg            <  1:225171/287‑1 (MQ=11)                                                                                                                                                                                                                                                                                           gTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTCGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCCGGCCTACACCGCTGTGAAGTGCCCCACCCCGTCGGTTGGATAAGGCCGTTACGCCGCATCCAACCACCAAccaccctgcctgcctcttaaacacat        >  2:392766/1‑263 (MQ=11)                                                                                                                                                                                                                                                                                                aCAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCCAACGTCCGAGCCGGTTGCCTGATGAGACGCTGgc  >  1:592407/1‑289 (MQ=18)                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                 CACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTAT‑ACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAAT‑TGACAACGC‑GGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGG‑TCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGC  >  NC_000913/2304253‑2304825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑