Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,304,796 T→C 13.1% intergenic (+405/+310) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,304,7960TC13.1% 116.9 / 6.1 54intergenic (+405/+310)eco/mqoecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base T (24/22);  new base C (4/4);  total (28/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCG  >  NC_000913/2304509‑2305078
                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                          
cTGTGAAGTGCGGCACCCCGTAGGTCAGTCAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGt                                                                                                                                                                                                                                                                                            >  1:370312/1‑288 (MQ=255)
cTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCAACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGt                                                                                                                                                                                                                                                                                            >  1:23464/1‑288 (MQ=255)
cTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATACAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGt                                                                                                                                                                                                                                                                                            >  1:821900/1‑288 (MQ=255)
      agggccgccccccgtggggcgagcAAGCCGGTCACGCCGCATCGTACATCCGACGCACGAGCCGGTGGCCTGAGGGAACACTGGGACGTCTTATCAGGCCTACACCGCTGTGAAGTGCTGCACCCCGTAGGTCGGATAAGTCGGTTACGCCGCATCCGACATCAAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGc                                                                                                                                                                                                                                                                                      <  2:538624/265‑1 (MQ=11)
          cGGCCCCCCGTGGGTCAGACAAGGCGGGCACGCCGCATCCGACATCCAACGCCCGGGCCGGTTGCCTGATGCGACGCTGGCGGGTCTTATCAGGCCTACAAAGCTGTGAAGTGCTCCACACCGTAGGTCGGATAAGGGGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGt                                                                                                                                                                                                                                                                                  <  1:1181450/288‑1 (MQ=255)
               ccccGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCGGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTg                                                                                                                                                                                                                                                                            >  1:974943/1‑289 (MQ=39)
                  cctAGGTCAGACACGCCGGCCAGGCCCCATCCCACAGCCAGCGCCCGGCCCGGTTGCCGAATGCGACCCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCGAGCCGGTTGCCTGATGCTACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATg                                                                                                                                                                                                                                                                         <  1:225171/287‑1 (MQ=11)
                            aCAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCCAACGTCCGAGCCGGTTGCCTGATGAGACGCTGgc                                                                                                                                                                                                                                                               >  1:592407/1‑289 (MQ=18)
                             taaGGCGGTCACGCGCACTCCGGCATCAAACGGCCGAGCCGGTTGCCTGTGGCGACGCTGGCGCGTCTTAGCGGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCGGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGgc                                                                                                                                                                                                                                                               <  2:370312/287‑1 (MQ=11)
                                 gCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGcgcg                                                                                                                                                                                                                                                            >  1:316882/1‑287 (MQ=14)
                                     tCACGCCACATCCAAAATCGAACGCCCGAGCCGGTGGCCTGAGGCGACGCTGGCGCGTCTTATCAGGGCGACACCGCTGTGAAGTGCACCACCCGGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGAGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                                                                                                                                                                                      <  1:772488/289‑1 (MQ=11)
                                      cACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                                                                                                                                                                                      <  1:964974/288‑1 (MQ=34)
                                                acacagaTCCGACGCCCGTGCCGGTTGCCTGATGCTCGGCGGGGCAGTCTTACGAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTAGGTGAAGGCGGTTACGCCGCATCCGAGATCTAACGCCCAAGCCGGTGGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTaca                                                                                                                                                                                                                                            <  2:1105511/283‑1 (MQ=11)
                                                    aCATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTCTCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAcc                                                                                                                                                                                                                                          >  1:1101560/1‑287 (MQ=38)
                                                         cAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCCAGCCGGTTGCCTGATGCCACGCTGGCGCGTCTTATCAGCCCTCCCCCGCTGTGAAGTACCCCACCCCGTAGGTTGGATAAGGCGGCTCCGCCGCATCCCACCTCCCACGCCCAACCCCGTTGCCTGAAGCCACCCCGGCGCGTCCTACCATGctacccccgccatgc                                                                                                                                                                                                                                   >  2:74527/1‑275 (MQ=11)
                                                          aaCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGaa                                                                                                                                                                                                                                  <  1:872156/289‑1 (MQ=40)
                                                             cgcggAGCCGGTTGCTTGGTCAGCGGCTGGAGCGTCTGATATGGCCTAACCGTCAGTTAAGTCCGCCAGCCCGTAGGTCGGAGAAGGCAGTTACCCCGCATCCGCGCCCGAACGCCCAAGCCTGTTGCCTAATGAGCCGCTGGTGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTAGGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTg                                                                                                                                                                                                                               <  2:1094489/285‑1 (MQ=11)
                                                                        ttGCCGGCTGCGACGCTGTCGCGTCTTAGCAGGCCTGCACCGCTGTGAAGTGCTCCACCCCGTGGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTACCGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCg                                                                                                                                                                                                                     <  2:316882/288‑1 (MQ=11)
                                                                                                cTTATCAGGCCTACACCGCTGGGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAcgccg                                                                                                                                                                                            <  1:5358/289‑1 (MQ=255)
                                                                                                             ccacgggtgggagcggcgcacccccGTAGGGCGGATAAGGCGGTTACGCCGCATCCGAAATCAACGGCCCAGGCCGGTGGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCt                                                                                                                                                                                <  2:934055/268‑1 (MQ=14)
                                                                                                                             cTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTgg                                                                                                                                                                                                                                                                >  1:1083094/1‑193 (MQ=34)
                                                                                                                                cACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCCCTACCCCGCTCTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTCACGCCGCATCCGACATCTAACGCCCCAGCCTGTTg                                                                                                                                                               >  2:210978/1‑287 (MQ=255)
                                                                                                                                  ccccGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCCGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGAt                                                                                                                                                                                                           >  1:789820/1‑241 (MQ=255)
                                                                                                                                     cGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACCCCCCTGTGAAGTACCCCACCCCCTAGGTCGGATAAGGCGCGTACGCCGCATCCTACATCTAACGCCCAAGCCGGTTGCCTgca                                                                                                                                                         >  2:825459/1‑286 (MQ=255)
                                                                                                                                            cGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCCCTGTGGAGTACCCCACCCCGCACTTCGGATACGTCGGTTCCGCCGCCCCCTACACTTCACGCCCGAGCAGGTTTcccgcccccaccc                                                                                                                                                  >  2:763468/1‑277 (MQ=11)
                                                                                                                                                    gCGGTTACGCCGCATCCGACAGCGAACTCCCAAGCGGGTTGCCGGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCAGGCGCGt                                                                                                                                          <  1:20650/288‑1 (MQ=255)
                                                                                                                                                        ttACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATTCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCACCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATc                                                                                                                                                                                                                                                     >  1:261327/1‑177 (MQ=25)
                                                                                                                                                        ttACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCTAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACCCCGCTGTGAAGTGCTCCACCCCGTCGGTCGGATCAGGCGGTTACCCCGCCTCCCACATCTAACTCCCACGCCTGTTGCCTGATCCgcaccttgctcgtccta                                                                                                                                      >  2:322061/1‑272 (MQ=11)
                                                                                                                                                              cgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCg                                                                                                                                                                                                                     >  1:1038921/1‑203 (MQ=40)
                                                                                                                                                              cgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCg                                                                                                                                                                                                                     <  2:1038921/203‑1 (MQ=40)
                                                                                                                                                                        aTCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCTGGCCTAAACCGCTGTGAAGTGCCCCACCCCGTAGGGCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCAAAACCGGTACCCTGATGTGACGCGCGCGCGTCTTACCAGGCCTACAcc                                                                                                                         >  1:555119/1‑285 (MQ=14)
                                                                                                                                                                            aaCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCt                                                                                                                                                                                >  2:708820/1‑226 (MQ=25)
                                                                                                                                                                            aaCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCt                                                                                                                                                                                <  1:708820/226‑1 (MQ=25)
                                                                                                                                                                                     gCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTa                                                                                                                                                                                                                                              <  1:426701/155‑1 (MQ=38)
                                                                                                                                                                                               tGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCtgtg                                                                                                                                                                                                                                    >  2:426701/1‑155 (MQ=38)
                                                                                                                                                                                                  tGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAgg                                                                                                                                                                                                                 <  2:574199/171‑1 (MQ=38)
                                                                                                                                                                                                            gcgcGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAgg                                                                                                                                                                                                       >  1:574199/1‑171 (MQ=39)
                                                                                                                                                                                                                          gCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACCCCCAAGCCGGTTGCCTGATGCGCCGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAATGCTCCCCCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCCAc                                                                   >  2:497902/1‑289 (MQ=255)
                                                                                                                                                                                                                                    cTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCCCTACACCGCTGTGGAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCAGCATCCAACATCTACCGc                                                          >  1:135833/1‑288 (MQ=255)
                                                                                                                                                                                                                                     tgtgAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACTCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATgcggcgtaaccgccttatccaacctacggggtg                                                         <  1:266677/288‑28 (MQ=11)
                                                                                                                                                                                                                                     caggAATTGCTCCACCCCGTAGGTTGGATAAGCCGGTTACGCCGCATCCGACATCAAACGTCCGCGCCGGTTGCCTGATGCGACGCTGGGGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGcc                                                         <  1:255404/285‑1 (MQ=255)
                                                                                                                                                                                                                                              cTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGtt                                               >  1:850235/1‑289 (MQ=40)
                                                                                                                                                                                                                                              cTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCCACACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCCGGCGCGTCTTATCAGGCCTCCCTCGCCGACACGTTCCCCACCCGCTAATGTGGATCATGCGTGTACGCCCCATCCCACATCCAACGCCCCAGCCGGtt                                               >  2:507550/1‑289 (MQ=11)
                                                                                                                                                                                                                                                   ccccGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGCTCCGACGCTGGCCCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTCGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTCGCGCGTCTTCTCAGGCCTACACCGCTCAGAAGTGCTCCCACCCGTAGGTTGGATAAGGCGGTTACGCCGCATACCAAACCTAACCCCCGAGCCGGTCGccg                                           >  2:255404/1‑287 (MQ=11)
                                                                                                                                                                                                                                                      cGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACCCTGGCCCGTCTTATCAGGCCTACACCGCTCTGCAGTGCTCCACCCCGTCGGTTGGATAAAGCCGTTACGCCGCCTCCGACCTCTACCCCCCGAGCCgcgttcccgct                                        >  2:1053357/1‑278 (MQ=11)
                                                                                                                                                                                                                                                        tAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGACCCGGTTGCCTGATGCg                                     >  1:623536/1‑289 (MQ=39)
                                                                                                                                                                                                                                                             tGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTCGGTTGGATAAGGCGGATACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCCAc                                   >  1:418353/1‑286 (MQ=255)
                                                                                                                                                                                                                                                               gATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTgg                              >  1:853760/1‑289 (MQ=37)
                                                                                                                                                                                                                                                                aTAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTa                                                                                                                                                                                                                                              <  1:767656/80‑1 (MQ=255)
                                                                                                                                                                                                                                                                     tcggtgtgtccgcACCCGACATCAACCGTCCGGGCGGGTTGCCGGACGCGACTCTGTGGGTTCTTGTCACGGCTACACCGCTGTCAAGTGCTACACCCCGTAGGTCGTACACGGCGGTTACGCCACATCCGACATCTATCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCTCGTCTTATCAGGCCTACACCGCTGAGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGt                         <  2:418353/279‑1 (MQ=11)
                                                                                                                                                                                                                                                                     gggcgacggccgcTCCCGACAGCAAACCCCAGGGCCGGGTGCGTAAGGCGACGCGGGCGCGACTTAGAAGGCCTAGACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCGGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGt                         <  2:821900/280‑1 (MQ=11)
                                                                                                                                                                                                                                                                      cGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGCGAAGTGCCCCACCCCGGAGGGCGGATAAGGCGGTTACGCCGCATCCAACATCTAACCCCCTAGCCGGTTTCCAGagcccactcttgcccgc                         >  2:977093/1‑271 (MQ=11)
                                                                                                                                                                                                                                                                         ttACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACCCCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATACGGCGGTTACGCCGCATCCAACATCTAACCCCCCAGCCGGTTGCCTCATGCGACGCTGGCGCGTCTTa                     >  2:1008253/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                              ccgcATCCGATTTCTAACGCCCAAGCCGGTTGCCGGGTGCGACGCTGGTGCGTGTTATCAGGCCTACACTGCTGTGAAGTGCTCAACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCa               <  1:1055696/289‑1 (MQ=11)
                                                                                                                                                                                                                                                                                   tCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAg                                                                                                                                                                                                                                                   <  2:1116117/56‑1 (MQ=35)
                                                                                                                                                                                                                                                                                        cATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCcaca      <  1:825459/288‑1 (MQ=39)
                                                                                                                                                                                                                                                                                           ccAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCg  <  1:763468/289‑1 (MQ=40)
                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                          
CTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCG  >  NC_000913/2304509‑2305078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: