Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,354,399 A→G 4.9% D46G (GAT→GGT)  glpB → anaerobic sn‑glycerol‑3‑phosphate dehydrogenase membrane anchor subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,354,3990AG 4.9% 351.0 / 3.7 107D46G (GAT→GGT) glpBanaerobic sn‑glycerol‑3‑phosphate dehydrogenase membrane anchor subunit
Reads supporting (aligned to +/- strand):  ref base A (50/51);  new base G (2/4);  total (52/55)
Fisher's exact test for biased strand distribution p-value = 6.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

CGCGCAATCTATCGAGCAACTTTCCACCTTCCTTAACGAACGCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTGCGCTCTACCTGGCTAAGTTCGCCAGAAGTCCCCGTCTGGCCGCTGCCCGCGAAGAA  >  NC_000913/2354117‑2354675
                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                    
cgcgCAATCTATCGAGCAACTTTCCACCTTCCTTAACGAACGCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGAttt                                                                                                                                                                                                                                                                                   >  1:791479/1‑286 (MQ=255)
     aaTCTATCGTGCAACTTTCCACCTTCCTTAACGAACGCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGcc                                                                                                                                                                                                                                                                           <  1:395035/289‑1 (MQ=255)
                cAACTTTCCACCTTCCTTAACGAACGCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGa                                                                                                                                                                                                                                                                 <  2:791479/288‑1 (MQ=255)
                   cTTTCCACCTTCCTTAACGAACGCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGAggg                                                                                                                                                                                                                                                              >  1:1079871/1‑288 (MQ=255)
                             tccttAACGAACGCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCa                                                                                                                                                                                                                                                           <  1:241887/281‑1 (MQ=255)
                             tccttAACGAACGCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCa                                                                                                                                                                                                                                                           >  2:241887/1‑281 (MQ=255)
                             tccttAACGAACGCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTg                                                                                                                                                                                                                                                    >  1:80412/1‑288 (MQ=255)
                             tccttAACGAACGCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGACTTGCTGAGCCATCTGCCAGATGGTCAACCGGTga                                                                                                                                                                                                                                                   >  2:117753/1‑289 (MQ=255)
                               cttAACGAACGCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTgaca                                                                                                                                                                                                                                                 <  1:1158099/289‑1 (MQ=255)
                                        cgCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTcac                                                                                                                                                                                                                                        >  2:180128/1‑289 (MQ=255)
                                         gCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTcaca                                                                                                                                                                                                                                       >  2:382323/1‑289 (MQ=255)
                                         gCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTcaca                                                                                                                                                                                                                                       <  1:1028158/289‑1 (MQ=255)
                                         gCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTcaca                                                                                                                                                                                                                                       <  1:655435/289‑1 (MQ=255)
                                         gCTGGAAAGGCGTGCAAACCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACTGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTcaca                                                                                                                                                                                                                                       <  2:213825/289‑1 (MQ=255)
                                            ggAAAGGCGTGCAACCCATCGCCGGGGGAGTTGCACTGCGCGAAAGCGAATTTACCCGCTGGGGTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGTTGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGt                                                                                                                                                                                                                                     <  2:82759/288‑1 (MQ=255)
                                                   cGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTCCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGa                                                                                                                                                                                                                             >  1:462311/1‑289 (MQ=255)
                                                     tGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGaa                                                                                                                                                                                                                            >  1:449699/1‑288 (MQ=255)
                                                       cAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCCTTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCCTCCAGATCGTCAACCGGTGCCAGACATTAACCGTGGACCGGAAt                                                                                                                                                                                                                           >  2:308081/1‑287 (MQ=255)
                                                            cATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCg                                                                                                                                                                                                                    <  1:663618/289‑1 (MQ=255)
                                                                  cTGGGGCGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGAGACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGCCATTCACAGTGGAGTGGAATCTTTGCGTcagc                                                                                                                                                                                                               <  1:256563/288‑1 (MQ=255)
                                                                  cTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTcagca                                                                                                                                                                                                              <  1:382323/289‑1 (MQ=255)
                                                                       gagaTGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTcag                                                                                                                                                                                                                <  1:375231/282‑1 (MQ=255)
                                                                        agaTGCACTGCGCGAAATCGAATTTACCCGATGGGTTTATCAGGGATTGTGTGGTCTTGAGAAGGTGCAGAAAGATGCTCTTTGATACGGTCATTATGGGCGGCGGCCTCGCCTGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCAc                                                                                                                                                                                                         <  1:524324/288‑1 (MQ=255)
                                                                        agaTGCACTGCGCGAAAGCGAATGTACCCGCTGGGTTTATCAGTGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCAc                                                                                                                                                                                                         <  1:247079/288‑1 (MQ=255)
                                                                            gCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGCGACCGACATTCACAGTGGACTGGAATCTTTGCGTCACCAGGCACCAGc                                                                                                                                                                                                     >  2:948948/1‑288 (MQ=255)
                                                                            gCACTGCGCCGAAGCGAAATTTCCCGCTGGGTTTATCAGGGATTTTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGcc                                                                                                                                                                                                    <  2:16320/289‑1 (MQ=255)
                                                                             cACTGCGCGAAAGCGAATTTACCCGGTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTTGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGcc                                                                                                                                                                                                    <  1:1161338/288‑1 (MQ=255)
                                                                              agtGCGCGAAAGCGTATTTACCCTGTGGGTTTATCAAGGATTGTGGAGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTCGCCTGCAACTGCAAAAACACGGCCTTCGCCGTGCCATTGTCACTCGTAGTCAAAGCGCTCTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATAGTCAACCGGTGACAGGCATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCTGccc                                                                                                                                                                                                   <  2:753049/286‑1 (MQ=255)
                                                                                 gcgcGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACAt                                                                                                                                                                                                                                            <  2:736371/244‑1 (MQ=255)
                                                                                 gcgcGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACAt                                                                                                                                                                                                                                            >  1:736371/1‑244 (MQ=255)
                                                                                 gcgcGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCAATGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGAAAGACATTCACAGTGGGATGGAAGCTTTGGGTAAGCAGGCACCAg                                                                                                                                                                                                      >  2:375231/1‑282 (MQ=255)
                                                                                  cgcgAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGGTTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCt                                                                                                                                                                                              >  1:1197109/1‑289 (MQ=255)
                                                                                  cgcgAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTg                                                                                                                                                                                                                                    <  2:297131/251‑1 (MQ=255)
                                                                                  cgcgAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTg                                                                                                                                                                                                                                    >  1:297131/1‑251 (MQ=255)
                                                                                   gcgAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCt                                                                                                                                                                                              <  1:132384/288‑1 (MQ=255)
                                                                                       aaGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACt                                                                                                                                                                                          <  2:155446/288‑1 (MQ=255)
                                                                                        aGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTcc                                                                                                                                                                                        <  1:244470/289‑1 (MQ=255)
                                                                                             aTTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTtct                                                                                                                                                                                   <  1:180128/289‑1 (MQ=255)
                                                                                                    cGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCca                                                                                                                                                                            <  1:982222/289‑1 (MQ=255)
                                                                                                         gggttATCAGGGATGGTGTGGGCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCAGTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGAACCAGCCCATCCt                                                                                                                                                                                              <  1:244071/263‑1 (MQ=255)
                                                                                                                   ggATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCAACAGCCCATCCTTACTCCCTTATCGAGACACAACGCGTGCTCGAt                                                                                                                                                             >  1:1153608/1‑289 (MQ=255)
                                                                                                                   ggATTGTGTGGTCTGCAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAGACACGGCCTGCGCTGTGCCATTGTCACTCCTGGTCACTGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGCTGGTCAACCGGTGACAGACATTCTCAGTGGACTGGAATCTTTGCGTCAGCAGGCACCACCCCATCCTTACTCCCTtc                                                                                                                                                                                    >  2:244071/1‑266 (MQ=255)
                                                                                                                    gATTGTGTGGTGTGGAGAAGGTGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGAt                                                                                                                                                             <  1:390499/288‑1 (MQ=255)
                                                                                                                    gATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGAt                                                                                                                                                             <  1:1083146/288‑1 (MQ=255)
                                                                                                                     aTTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTga                                                                                                                                                                                                                                                   <  2:370879/201‑1 (MQ=255)
                                                                                                                     aTTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTga                                                                                                                                                                                                                                                   >  1:370879/1‑201 (MQ=255)
                                                                                                                     aTTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGAtc                                                                                                                                                            >  1:170192/1‑288 (MQ=255)
                                                                                                                         tgtgGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCt                                                                                                                                                       >  1:847961/1‑289 (MQ=255)
                                                                                                                             gTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTcagc                                                                                                                                                                                                               <  2:677934/229‑1 (MQ=255)
                                                                                                                                    gaAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGgcgc                                                                                                                                             <  1:948948/288‑1 (MQ=255)
                                                                                                                                       ggAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAgg                                                                                                                                          >  1:448208/1‑288 (MQ=255)
                                                                                                                                            gaaaagagcggcTTTGATACTGCCATTATGGGCTGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAGCACGGCCTGCGCTGTGACATTGTCTTTCGTGGTCAAAGCACACTGCATTTCTCTGCCGGGTCGCTGGAAGGGCAGTGCCATCTGCCAGATGGTCAACCGGTGACACGCAGTCACAGTGGACTGGAATCTCTGCGTCAGCAGGCACCAGCCCATCCTTCCTCCCTTCTCGAGCCACAACGCGTGCTCGATCGCGCTTGCCAGGCGCAGGCATTa                                                                                                                                     <  2:27420/278‑1 (MQ=255)
                                                                                                                                            aGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCCCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAGTCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCCCGATCCCGCTTCCCAGGCGCAGGGTTTaa                                                                                                                                    >  2:91309/1‑289 (MQ=255)
                                                                                                                                                   tGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGcc                                                                                                                                                                                                    <  1:444008/218‑1 (MQ=255)
                                                                                                                                                   tGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGcc                                                                                                                                                                                                    >  2:444008/1‑218 (MQ=255)
                                                                                                                                                            aTACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTgc                                                                                                                    >  1:910617/1‑289 (MQ=255)
                                                                                                                                                            aTACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTgc                                                                                                                    >  1:617479/1‑289 (MQ=255)
                                                                                                                                                             tACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGt                                                                                                                                                                                                                                                     <  1:11179/159‑1 (MQ=255)
                                                                                                                                                             tACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGt                                                                                                                                                                                                                                                     >  2:11179/1‑159 (MQ=255)
                                                                                                                                                             tACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTgcg                                                                                                                   <  1:993242/289‑1 (MQ=255)
                                                                                                                                                              aCTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTTGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTgcgc                                                                                                                  <  1:1142194/289‑1 (MQ=255)
                                                                                                                                                              aCTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTgcgc                                                                                                                  <  2:170192/289‑1 (MQ=255)
                                                                                                                                                                tGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAAc                                                                                                                                                                        <  2:409492/233‑1 (MQ=255)
                                                                                                                                                                tGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAAc                                                                                                                                                                        >  1:409492/1‑233 (MQ=255)
                                                                                                                                                                  tCATTATGGGCGGCGGCCTCGCCGGCTTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGGCAACCGGTGACAGACATTTCCAGTGGACTGGAATCTTTGCGTCAGCAGGCACAAGCCCATCATTACTCCCTTCTCGAGCCACAACGCGTGCTCGAACTCGCTTGCCAGGCGCAGGCAGTAATACACGAAAGCGGTCCGCAAtt                                                                                                              >  2:1142194/1‑289 (MQ=255)
                                                                                                                                                                  tCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAAtt                                                                                                              >  1:100154/1‑289 (MQ=255)
                                                                                                                                                                      tATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCCATTTCAg                                                                                                          >  1:1019964/1‑289 (MQ=255)
                                                                                                                                                                        tGGGCCGCTGCCTCGCCGGATTACTCGGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAggg                                                                                                        <  2:100154/289‑1 (MQ=255)
                                                                                                                                                                            cggcggcCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGGTTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCACTTGCAGGGCAGc                                                                                                    >  2:407563/1‑289 (MQ=255)
                                                                                                                                                                            cggcggcCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCt                                                                                                                                                                                              >  2:158504/1‑199 (MQ=255)
                                                                                                                                                                            cggcggcCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCCCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGACTTAATCGCTGAAAGCGGTGCGCAATTGCCGTGCAGc                                                                                                    >  2:591446/1‑289 (MQ=255)
                                                                                                                                                                                               gtcagatgCCGGCAACTGCAACAACCCGGCTGGCGTGGTGCCGTTGCCACTCGTGGCCAAAGCGCACTGCATTTCACAACCGGATCGCTTGATTTGAGTAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAg                                                                                  <  2:1079871/281‑1 (MQ=255)
                                                                                                                                                                                                  tgtgGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCATTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCTTTCTCGAGCCACCACCATTCCTCGATCCCGCTTGCCAGGCGCCTGCATTAATCGCTTAAAGCGGTTCCCACTCTCGGGGCCACTTAGAACTTTCTAACAAGCgcg                                                                              >  2:1206691/1‑287 (MQ=255)
                                                                                                                                                                                                  tgtgGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCCCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCTTAGAACTTGCGCACCAGCggg                                                                              >  1:810761/1‑289 (MQ=255)
                                                                                                                                                                                                    tgGCCTGCAACTGCAAAAACACGGCATGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCAACCTTACCCCCTTCTCGAGCCACAACGCGTCCCCGATCTCGATAGCCAGGCCCAGGAATTACTCGACACAAGCGGGGCGCAATTTAAGGGCAGCGTAGAACTTGCTCACCAGAGGGtt                                                                            >  2:455478/1‑289 (MQ=255)
                                                                                                                                                                                                      gCCTGCAACTGCAACAACACGGCCTGCGATGTGCAATTGTCACTCTTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGGTTTGCTTGGCCAGCTGCCAGATGGTCAACCGGGAACGGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCAACCGTACTCCCGTCTCGAGCCACAACGCGGGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATAGCTGAAAGCGGCGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTa                                                                           <  2:1197109/288‑1 (MQ=255)
                                                                                                                                                                                                        ctgcaactgcaaAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTAcg                                                                         <  1:591446/288‑1 (MQ=255)
                                                                                                                                                                                                         tgcaactgcaaAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTAcgcc                                                                       >  1:1057319/1‑289 (MQ=255)
                                                                                                                                                                                                            aactgcaaAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCt                                                                    <  2:80412/289‑1 (MQ=255)
                                                                                                                                                                                                                 caaAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGc                                                                >  2:85252/1‑288 (MQ=255)
                                                                                                                                                                                                                              tGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGGTTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTGCGCTCTAc                                                  <  1:407563/289‑1 (MQ=255)
                                                                                                                                                                                                                                cgcTGTGCCATTGTCACTCGTGCTCAAACCGCACTGCACTTCCCATCCGGATCGCTGGAATTGCTGAGCCATCTGCCAGATGGTCAACTGGTGACAGACATTCCCAGTGGACTGGAATCTTTACGTCCGCAGGCACCAGCCCATCCTTACTCCCTTCTCGACCCACACCCCGTGCTCGATCTCGCTTGCCAGGCGCCCGCATTAATCGCTTAAAGCGGTCCGTATTTGCAGGGCAGCGTAGAACTTGCCCACCACAGGGTTACGCCGCTCGGCACTCTGCGCCCTAcc                                                 >  2:331622/1‑288 (MQ=255)
                                                                                                                                                                                                                                         aTTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGGTCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTGCGCTCTACCTGGCTAAGtt                                       <  1:492775/289‑1 (MQ=255)
                                                                                                                                                                                                                                          ttGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTGCGCTCTAc                                                  <  2:1011217/277‑1 (MQ=255)
                                                                                                                                                                                                                                          ttGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTGCGCCCTAc                                                  >  1:1011217/1‑277 (MQ=255)
                                                                                                                                                                                                                                            gTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTGCGct                                                      <  2:311597/271‑1 (MQ=255)
                                                                                                                                                                                                                                            gTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTGCGct                                                      >  1:311597/1‑271 (MQ=255)
                                                                                                                                                                                                                                               aCTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAg                                                                                                                                           >  2:67979/1‑183 (MQ=255)
                                                                                                                                                                                                                                               aCTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAg                                                                                                                                           <  1:67979/183‑1 (MQ=255)
                                                                                                                                                                                                                                               aCTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGATCACCAGCGGGTTACGCCGCTCGGCAATCTGCGCAATACCTGGCCAAGCTCGCCAg                                 >  2:492775/1‑289 (MQ=255)
                                                                                                                                                                                                                                                     ggTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTGCGCTCTACCTGGCTAAGTTCGCCAGAAGTc                            <  2:449699/288‑1 (MQ=255)
                                                                                                                                                                                                                                                               cACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTGCGCTCTACCTGGCTAAGTTCGCCAGAAGTCCCCGTCTGGcc                 >  2:1122894/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                         gtcttgcgtaTCGCTGGATTTGCTGAGCCAGCTTCAGGATGGTCAACCGGTGACAGACATTCACAGTGGGCTGGAATCTTGGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTGCGCTCTACCTGGCTAAGTTCGCCAGAAGTCCCCGTCTGGCCGCTGCCcgcg       <  2:722527/280‑1 (MQ=255)
                                                                                                                                                                                                                                                                         ctcATCCGGATCGCTGAATTTGCGGAGCCAGCGGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTGCGCTCTACCTGGCTAAGTTCGCCAGAAGTCCCCGTCTGGCCGCTGCCcgcg       <  2:400550/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                            aTCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTGCGCTCTACCTGGCTAAGTTCGCCAGAAGTCCCCGTCTGGCCGCTGCCCGCgaag    >  1:813481/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                              ccGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGACTCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGt                                                                                                  <  2:587476/193‑1 (MQ=255)
                                                                                                                                                                                                                                                                              ccGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGt                                                                                                  >  1:587476/1‑193 (MQ=255)
                                                                                                                                                                                                                                                                              ccGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTGCGCTCTACCTGGCTAAGTTCGCCAGAAGTCCCCGTCTGGCCGCTGCCCGCgaagaa  <  1:85252/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                              ccGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTGCGCTCTACCTGGCTAAGTTCGCCAGAAGTCCCCGTCTGGCCGCTGCCCGCgaagaa  >  1:1035620/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                              ccGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTGCGCTCTACCTGGCTAAGTTCGCCAGAAGTCACCGTCTGGCCGCTGCCCGCgaagaa  >  1:647373/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                    
CGCGCAATCTATCGAGCAACTTTCCACCTTCCTTAACGAACGCTGGAAAGGCGTGCAACCCATCGCCTGGGGAGATGCACTGCGCGAAAGCGAATTTACCCGCTGGGTTTATCAGGGATTGTGTGGTCTGGAGAAGGAGCAGAAAGATGCGCTTTGATACTGTCATTATGGGCGGCGGCCTCGCCGGATTACTCTGTGGCCTGCAACTGCAAAAACACGGCCTGCGCTGTGCCATTGTCACTCGTGGTCAAAGCGCACTGCATTTCTCATCCGGATCGCTGGATTTGCTGAGCCATCTGCCAGATGGTCAACCGGTGACAGACATTCACAGTGGACTGGAATCTTTGCGTCAGCAGGCACCAGCCCATCCTTACTCCCTTCTCGAGCCACAACGCGTGCTCGATCTCGCTTGCCAGGCGCAGGCATTAATCGCTGAAAGCGGTGCGCAATTGCAGGGCAGCGTAGAACTTGCTCACCAGCGGGTTACGCCGCTCGGCACTCTGCGCTCTACCTGGCTAAGTTCGCCAGAAGTCCCCGTCTGGCCGCTGCCCGCGAAGAA  >  NC_000913/2354117‑2354675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: