Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,004,822 A→C 54.0% N321K (AAT→AAG ygeV ← putative sigma‑54‑interacting transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,004,8220AC54.0% 74.2 / 235.9 169N321K (AAT→AAGygeVputative sigma‑54‑interacting transcriptional activator
Reads supporting (aligned to +/- strand):  ref base A (37/41);  new base C (48/43);  total (85/84)
Fisher's exact test for biased strand distribution p-value = 5.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

Only 100 of 169 total aligned reads displayed.

AATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCACGTGCCTCAATAGCGCGCAGTAATTTAGCCTGCAACATTAATGGCATATCACCTATTTCATCGAGAAACAGCGTGCCCGTATTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCACTTTTATCGTCCCATGA  >  NC_000913/3004536‑3005109
                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                
aaTTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCACGTGCATCAATAGCGAGCAGTAATTTAGCCTGCAACATTAATGGCATATCACATATTTCATCGAGAAACAGCGTGACCGTATTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACCGTTCG‑‑CTCTCCAGAAGCCGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGCTTAtt                                                                                                                                                                                                                                                                                                >  1:1381549/1‑289 (MQ=255)
     tGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCACGTGCCTCAATAGCGCGCAGTAATTTAGCCTGCAACATTAATGGCATATCACCTATTTCATCGAGAAACAGCGTGCCCGTATTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTc                                                                                                                                                                                                                                                                                           <  1:286194/289‑1 (MQ=255)
             tGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCACGTGCCTCAATAGCGCGCAGTAATTTAGCCTGCAACATTAATGGCATATCACCTATTTCATCGAGAAACAGCGTGCCCGTATTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCATAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTc                                                                                                                                                                                                                                                                                   <  2:1412649/289‑1 (MQ=255)
               cAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCACGTGCCTCAATAGCGCGCAGTAATTTAGCCTGCAACATTAATGGCATATCACCTATTTCATCGAGAAACAGCGTGCCCGTATTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCa                                                                                                                                                                                                                                                                                  >  1:250047/1‑288 (MQ=255)
                         gCGAATGTCGACTTGTATTGGGCTACTGGCACCAATTGGCAGAATTTCATGTGCCTCAATAGCGCGCAGTAATTTAGCCTGCAACATTACTGGCATATCACCTATTTCATCGAGAAACAGCGTGCCCGTATTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGAtt                                                                                                                                                                                                                                                                       >  2:838581/1‑289 (MQ=255)
                                     ttGTATTGGGCTACTGGCACCAATCGGCAGAATTTCACGTGCCTCAATAGCGCGCAGTAATTTAGCCTGCAACATTAATGGCATATCACCTATTTCATCGAGAAACAGCGTGCCCATATTCGCCTCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGcgacg                                                                                                                                                                                                                                                           <  1:739226/289‑1 (MQ=255)
                                                                  aaagttcaatggcctcgagagcggcaagtaagtaAGCCTTCAACCTTAATGACATATCACCTATTTGATCGTTAAACAGCGTGCCCGTATTCGCCGCCTGAATCAA‑‑CCATGTTTTACCGTTGGCAGAACCGCCAGTAAATTCACCTTGAACATAACCGAACAGTTCG‑‑CTACCCAGAAGCTGCTCCGGAATCGCGCCACAGTTGATAGCAATAAATGGTTTATTCCGTCTTCCGCTCAACTTATGGATTGCTCGGGCTACGACTTCTTTACCCGTGCCGTTTTCaccatc                                                                                                                                                                                                                              <  2:634552/263‑3 (MQ=255)
                                                                    aTTTCACGTGCCTCAATAGCGCGCAGTAATTTAGCCTGCAACATTAATGGCATATCACCTATTTCATCGAGAAACAGCGTGCCCGTATTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAACGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCaccaac                                                                                                                                                                                                                              <  2:135236/287‑1 (MQ=255)
                                                                                  aaTAGCGCGCAGTAATTTAGCCTGCAACATTAATGGCATATCACCTATTTCATCGAGAAACAGCGTGCCCGTATTCGCCGCCTGAATCCA‑‑CCCTGTTTTACCATTGGCAGAAGCGCCAGTAAATGCACCTTTAACATACCCGCACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGCTTGCACGGGCGACGCCTTNNNNACCCGTTCCGCttg                                                                                                                                                                                                                                     >  2:1157297/1‑265 (MQ=255)
                                                                                    tatcggccAGTAATTTAGCTTGCAACATAGAAGGCATATCAACCATTTCATCGAGAAACGGCGTGCCCGTATTCGCCGCCTGAATCCACCCCCTGTTTTACCGTTTGCAGATGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCGCAGTTGATATCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGTCGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGAt                                                                                                                                                                                                              <  2:660740/282‑1 (MQ=255)
                                                                                       cgcgcAGTAATTTAGCCTGCAACATTAATGGCATATCACCTATTTCATCGAGAAACAGCGTGCCCGTATTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCt                                                                                                                                                                                                         <  2:980976/289‑1 (MQ=255)
                                                                                         cgcAGTAATTTAGCCTGCAACATTAATGGCATATCACCTATTTCATCGAGAAACAGCGTGCCCGTATTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTATTCCGTCTTCCGCTCAACtt                                                                                                                                                                                                                                                                              <  1:811649/218‑1 (MQ=255)
                                                                                         cgcAGTAATTTAGCCTGCAACATTAATGGCATATCACCTATTTCATCGAGAAACAGCGTGCCCGTATTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTATTCCGTCTTCCGCTCAACtt                                                                                                                                                                                                                                                                              >  2:811649/1‑218 (MQ=255)
                                                                                           cAGTAATTTAGCCTGCAAAATTAATTGCATTTCACCTATTTCACCGGAAAACAGCGTGCCAGTATTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCTGCACAGTTGATAGCAATAAAGGGTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTggg                                                                                                                                                                                                      <  2:1352636/288‑1 (MQ=255)
                                                                                               actttAGCCTGCAACATTAATGTCATATCACCTATTGCATCGAGAAACAGCGTGCCCGTATTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCaa                                                                                                                                                                                                 <  1:1157297/287‑1 (MQ=255)
                                                                                               aaTTTAGCCTGCAACATTAATGGCATATCACCTATTTCATCGAGAAACAGCGTGCCCGTATTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCaa                                                                                                                                                                                                 <  1:1351342/289‑1 (MQ=255)
                                                                                               aaTTTAGCCTGCAACATTAATGGCATATCACCTAGTTCATCGAGAAACAGCGTGCCCGTATTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGTAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTAGGTGCaa                                                                                                                                                                                                 <  1:1360/289‑1 (MQ=255)
                                                                                                aTTTCGCCTGAAACATGAATGGCATATCACCGATTTCATCGAGAAACAGCGTGCCCTTATTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCaccaac                                                                                                                                                                                                                              <  2:1369193/259‑1 (MQ=255)
                                                                                                      ccTGCAACATTAATGGCATATCACCTATTTCATCGAGAAACAGCGTGCCCGTATTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCt                                                                                                                                                                                          >  1:410576/1‑289 (MQ=255)
                                                                                                      ccTGCAACATTAATGGCATATAACCTATTTCATCGAGAAACAGCGTGCCCGTATTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAAAATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGAGGCCCAGTTGATAGCAATAAAGGGTTTATTCCGTCTTCCGCCCAACTTATGGATTGCACGGGCGACGACTTCTTTACTCGTGCCGCTTTCCCCAACCACCATAAAGCTTGATGGGCTGGGGCCAATATGGCt                                                                                                                                                                                          >  2:1360/1‑289 (MQ=255)
                                                                                                        tGCAACATTAATGGCATATCACCTATTTCATCGAGAAACAGCGTGCCCGTATTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTaa                                                                                                                                                                                       >  2:235773/1‑289 (MQ=255)
                                                                                                          cAACATTAATGGCATATCACCTATTTCATCGAGAAACAGCGTGCCCGTATTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATg                                                                                                                                                                                     >  2:1275125/1‑289 (MQ=255)
                                                                                                          cAACATTAATGGCATATCACCTATTTCATCGAGAAACAGCGTGCCCGTATTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTATTCCGTCTTCCGCCCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACg                                                                                                                                                                                                                    >  1:1369193/1‑259 (MQ=255)
                                                                                                                tAATGGCATATCACCTATTTCATCGAGAAACAGCGTGCCCGTATTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGt                                                                                                                                                                               >  1:1064947/1‑289 (MQ=255)
                                                                                                                            actcaTTTCATCGAGAAACGGCGTGCCCGTATTCGCGGCCTAAATCAA‑‑CCCGTTTTTCCCTTGGGCGAAAGCGCCGTTAAATGCACCTTTAACATAACCGAAAGGTTC‑‑CTCTCCCGGAAGCGCCTCCGGAATCCCGGCCAAGTTAATAGAAATAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCa                                                                                                                                                                  <  2:116682/285‑1 (MQ=255)
                                                                                                                             ccTATTTCATCGAGAAACAGCGTGCCCGTATTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCa                                                                                                                                                                  <  2:320242/289‑1 (MQ=255)
                                                                                                                                 tttCATCGAGAAACAGCGTGCCCGTATTCGCCTCCTGAATCAA‑‑CCCTGATGTACCGTTGGCAGAAGCGCCAGTAAATGCACCTATAACATAACCGAACAGTTCG‑‑CACTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTATTCCGTCGTCCGCTCAACTTATGGATTGCACGGGCGACGAATTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCt                                                                                                                                                                                                         <  2:126347/247‑1 (MQ=255)
                                                                                                                                 tttCATCGAGAAACAGCGTGCCCGTATTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAac                                                                                                                                                              >  2:585561/1‑289 (MQ=255)
                                                                                                                                  ttCATCGAGAAACAGCGTGCCCGTATTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAaca                                                                                                                                                             <  2:915859/289‑1 (MQ=255)
                                                                                                                                  ttCATCGAGAAACAGCGTGCCCGTATTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGATCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTATTCCGTATTCCGCTCAACTTATGGATTGCACGGGCGAAGACTTCTTTACCCGTTCCGCTTTTACCCACCACCATAAAGACGGATGTGCTGGGTGCGTAAGGCTCACTGACGCGTTCTAATTGCCTCATAcca                                                                                                                                                             >  2:532887/1‑289 (MQ=255)
                                                                                                                                     ctCGATAGACAGCGTGCGCGGATTCGCCGCCTGAATCAA‑‑CACTGTTTTACCGTTGGCAGACGCGCCAGTAAATGCACCTTTATCATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAAGCGCTGCACAGTTGATAGCAATAAAGGGTTGATTCCGTCTTCCGCGCAACTTATGGATTGCACGGGCGACGAGTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATGGCCGCATAACACgg                                                                                                                                                          <  2:763188/288‑1 (MQ=255)
                                                                                                                                     aTCGAGAAACAGCGTGCCCGTATTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCGCTCTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAacac                                                                                                                                                            >  2:1105787/1‑289 (MQ=255)
                                                                                                                                     aTCGAGAAACAGCGTGCCCGTATTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACgg                                                                                                                                                          <  1:1135622/289‑1 (MQ=255)
                                                                                                                                      tCGAGAAACAGCGTGCCCGTATTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACgg                                                                                                                                                          >  2:1191481/1‑288 (MQ=255)
                                                                                                                                      tCGAGAAACAGCGTGCCCGTATTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCCCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCTTaaataggc                                                                                                                                                         >  2:1056773/1‑283 (MQ=255)
                                                                                                                                          gaAACAGCGTGCCCGTATTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACt                                                                                                                                                      <  1:1191481/288‑1 (MQ=255)
                                                                                                                                                   tGCCCGTCTTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGccaacc                                                                                                                                              >  2:995357/1‑287 (MQ=255)
                                                                                                                                                   tGCCCGTATTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGAACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCCCAACTTATGGATTGCACGGGCGACGAATTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTCCCGCATAAAACGGCACTCCccaaccaa                                                                                                                                            >  2:576881/1‑289 (MQ=255)
                                                                                                                                                    tccctgaTTCGACACCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGAACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGccaaccaa                                                                                                                                            <  2:410576/282‑1 (MQ=255)
                                                                                                                                                     cccGTATTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTATTCCGTCTTCCGCTCAACtt                                                                                                                                                                                                                                                                              <  2:1011698/158‑1 (MQ=255)
                                                                                                                                                     cccGTATTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTATTCCGTCTTCCGCTCAACtt                                                                                                                                                                                                                                                                              >  1:1011698/1‑158 (MQ=255)
                                                                                                                                                       cGTATTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATACCGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGt                                                                                                                                        <  1:838581/289‑1 (MQ=255)
                                                                                                                                                        gTATTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGtt                                                                                                                                       <  1:977955/289‑1 (MQ=255)
                                                                                                                                                         tATTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCCTACCGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATaa                                                                                                                                                               >  1:286163/1‑264 (MQ=255)
                                                                                                                                                          aTTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCGCTCTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGtt                                                                                                                                       <  1:1105787/289‑1 (MQ=255)
                                                                                                                                                          aTTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCa                                                                                                                                     <  1:712021/289‑1 (MQ=255)
                                                                                                                                                          aTTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCa                                                                                                                                     <  1:630128/289‑1 (MQ=255)
                                                                                                                                                                   cTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCTCTTTCACCAAccacca                                                                                                                                                                                                                         >  1:118319/1‑197 (MQ=255)
                                                                                                                                                                              cccTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGt                                                                                                                   <  2:250047/289‑1 (MQ=255)
                                                                                                                                                                                  gTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCa                                                                                                                                                        >  2:352010/1‑248 (MQ=255)
                                                                                                                                                                                  gTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGATCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACTGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGtcaat                                                                                                               >  1:618793/1‑285 (MQ=255)
                                                                                                                                                                                    tgtACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCATAAGCTGCTCGGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTGACCCGTGCCGCTTTCACCAGCCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCAtt                                                                                                              <  1:87713/286‑1 (MQ=255)
                                                                                                                                                                                        ccGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCt                                                                                                          >  1:1341166/1‑288 (MQ=255)
                                                                                                                                                                                        ccGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑ATCGCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTCCCCGTGCCGCTTTCACCAACCACCGTAACGCGTGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGACCCCCCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTa                                                                                                         >  2:683725/1‑289 (MQ=255)
                                                                                                                                                                                             ggCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTATTCCGTCTTCCGCTCCACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAAACACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTCCCGCATACCACGGCACTCGCCAACCAATTGTTCAATTTGCGGtttctcctgtctcttatacccacct                                                                                                    >  2:646500/1‑266 (MQ=255)
                                                                                                                                                                                                   aGCGCCAGTAAATGCACCTTTAATAGAACCAACTAGTGCG‑‑CTCTCCTGAAGCTGCTCCGGAATCGCGGCACAGGTGATAGCAATAAAGGGTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGAGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTg                                                                                              <  1:266747/289‑1 (MQ=255)
                                                                                                                                                                                                       ccAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTAt                                                                                          <  1:623162/289‑1 (MQ=255)
                                                                                                                                                                                                       ccAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATAAGGTGCATTTGCTACATCAAAACTGGTAt                                                                                          >  1:488700/1‑289 (MQ=255)
                                                                                                                                                                                                                           taaaccagccAGTTCG‑‑CTCGCCAGAAGCTGCTCCGGAATCTCGGCACAGTTGATAGCAATAAAGGGTTTATTCCGTCTTCAGCTCAACTTATGGATAGCACGTGCTACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGTTGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCAtt                                                                      <  2:113481/280‑1 (MQ=255)
                                                                                                                                                                                                                              aCCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTtcat                                                                                                                        <  1:1393324/236‑1 (MQ=255)
                                                                                                                                                                                                                              aCCGAACAGTTCG‑‑CTCCCCAGAAGCTGCTACGGAATCCCGGAACAGTTGGTAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTTTGTATTTAACGGGCGACGACGTCTTTGCCCGTGCCGCTTTCATCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGTTAATGAGTCGTCTTAATTGCCCCATAACCCGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGTATGGGCAAACGCCAtttac                                                                   >  2:173127/1‑286 (MQ=255)
                                                                                                                                                                                                                               ccGAACAGTTCG‑‑CTCTCCAGAGGCGGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCGTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGc                                                                                                                               <  2:18986/228‑1 (MQ=255)
                                                                                                                                                                                                                               ccGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGAACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACCCGCCAATCAATTGTTCAATATGAGGTTCATGAGGTGCATTTGGTACAGAAAAACTGGTATGCGCGTGGTGAAACg                                                                           >  1:1268011/1‑280 (MQ=255)
                                                                                                                                                                                                                                  aaCAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAaata                                                               >  1:665205/1‑289 (MQ=255)
                                                                                                                                                                                                                                  aaCAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAaata                                                               >  1:322313/1‑289 (MQ=255)
                                                                                                                                                                                                                                    cAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCACTACGCATAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATGGTTCAATATTCGGTTCATCCGGTTCATTGGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAaataat                                                             >  2:171722/1‑289 (MQ=255)
                                                                                                                                                                                                                                       ttCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGt                                                          >  1:557696/1‑289 (MQ=255)
                                                                                                                                                                                                                                       ttCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGt                                                          <  2:949173/289‑1 (MQ=255)
                                                                                                                                                                                                                                       ttCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGt                                                          >  2:548672/1‑289 (MQ=255)
                                                                                                                                                                                                                                       ttCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACCCGCCAACCAATTGTTCAATATTCGGTTCATCCGGTGCATTTGCTACCGACAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATCAttc                                                           >  2:684412/1‑287 (MQ=255)
                                                                                                                                                                                                                                        tCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGt                                                          <  1:576881/288‑1 (MQ=255)
                                                                                                                                                                                                                                         cG‑‑CTCTCTAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAg                                                                                                                     >  1:18986/1‑228 (MQ=255)
                                                                                                                                                                                                                                         cG‑‑CTCTCTAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCg                                                        >  1:1742/1‑289 (MQ=255)
                                                                                                                                                                                                                                         cG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAATAATAATTGTCg                                                        >  1:656498/1‑289 (MQ=255)
                                                                                                                                                                                                                                         cG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCg                                                        >  1:1049773/1‑289 (MQ=255)
                                                                                                                                                                                                                                               ctcCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGccc                                                    <  1:99746/289‑1 (MQ=255)
                                                                                                                                                                                                                                                tcCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCt                                                   <  2:1064947/289‑1 (MQ=255)
                                                                                                                                                                                                                                                   cAGAAGCTGCTCCGGAATCGCAGCACGATTGATAGCATTAAAGGGTTTCTTCCTTCTTCCGCTCAACTTATGGATTGCACGAGCGACGACTTCTTTACCCGTGCCGCGTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACTCGGCACTCGCCAACCAATTGTTCAATATGCTGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGa                                                 <  2:50661/289‑1 (MQ=255)
                                                                                                                                                                                                                                                           gctCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCa                                                                                                                                                                                                                                                                    >  2:603234/1‑71 (MQ=255)
                                                                                                                                                                                                                                                           gctCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCaccaac                                                                                                                                                                                                                              <  2:1070521/109‑1 (MQ=255)
                                                                                                                                                                                                                                                           gctCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTAt                                          >  1:173597/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                ggAATCGCGGCACAGTTGATAGCAATAAAGGGTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAAtt                                    <  1:585561/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                     cgcgGCACAGTTGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGATTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCa                               <  1:1311202/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                tGATAGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGTGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAAtt                     <  1:65795/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                                tGATAGCAATAAAGGGTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAAtt                     <  1:1275125/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                                    tgCAATAAAGGGTTTCTTCCCTCGTCTGCGCAGCTGATGGATTGCACGGGCGCCGACTGCTTTACCCGTGCCGCTGTAACCTACAACCATAACGCTGGCTTGGCTTGGTGCAAGACGACTAGTTAGTCGTTTTAAGTGCCGCATAACACGGCACTCGCCAACCAATTGTTCCAGATGCTGTTCATCAGGTGAATTTGCTACAGAAAAACTGGTATGAGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCAc                  <  2:616068/286‑1 (MQ=255)
                                                                                                                                                                                                                                                                                    aGCAATAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCgg                                                                                                                             >  2:1372013/1‑180 (MQ=255)
                                                                                                                                                                                                                                                                                         tAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCCCTCGCTAACCAATTGTTCAATATGCGGTTCATCAGGTTGATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATAAAAAATAATTGTCGGCCCTGAATGTTATGCACTTGACCAATGATTAATTCCCtttttt            >  2:1198031/1‑286 (MQ=255)
                                                                                                                                                                                                                                                                                         tAAAGGGTTTCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCACTTTTATc           >  1:577110/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                         tAAAGGGTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGcca                                                                                                                                                 >  1:955369/1‑155 (MQ=255)
                                                                                                                                                                                                                                                                                         tAAAGGGTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTg                                                                                                            <  2:625471/192‑1 (MQ=255)
                                                                                                                                                                                                                                                                                         tAAAGGGTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCACTTTTa             >  1:949407/1‑287 (MQ=255)
                                                                                                                                                                                                                                                                                         tAAAGGGTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCACTTTTATc           >  1:910830/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                         tAAAGGGTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCACTTTTATc           >  1:109532/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                         tAAAGGGTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCAGCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGTTGAAACGCCAATAAAAATAATTATCGGCTCTGAATGTTATGCAATTGACTCATGATTAATTCACTTTTAtt           >  2:456995/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                                tttCTTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCACTTTTATCGTCCCa     <  1:235773/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                tttATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCACTTTTATCGTCCCa     <  2:940891/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                 ttATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCg                                                        >  1:926483/1‑236 (MQ=255)
                                                                                                                                                                                                                                                                                                 ttATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCACTTTTATCGTCCCATg   <  1:145464/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                 ttATTCCGGCTTCCGCTCAACTTTTGGATTGCACTGGCGACGACTTCTTTGCCCGTGCCGCTTTCACCAACCCCCCTAACGCTTGATGATCTTGGTGCAATACGGCTAATGAATCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGGTCATCAGGTGCATTTGCTACAGAAAATCTGGTATGCGATTGGTGAAAGGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACTAATGATTAATTCACTTTTATCGTCCCATg   <  1:532887/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                
AATTCTGATTAGTTGCAGAAATGATGCGAATGTCGACTTGTATTGGGCTACTGGCACCAATCGGCAGAATTTCACGTGCCTCAATAGCGCGCAGTAATTTAGCCTGCAACATTAATGGCATATCACCTATTTCATCGAGAAACAGCGTGCCCGTATTCGCCGCCTGAATCAA‑‑CCCTGTTTTACCGTTGGCAGAAGCGCCAGTAAATGCACCTTTAACATAACCGAACAGTTCG‑‑CTCTCCAGAAGCTGCTCCGGAATCGCGGCACAGTTGATAGCAATAAAGGGTTTATTCCGTCTTCCGCTCAACTTATGGATTGCACGGGCGACGACTTCTTTACCCGTGCCGCTTTCACCAACCACCATAACGCTGGATGGGCTGGGTGCAATACGGCTAATGAGTCGTTTTAATTGCCGCATAACACGGCACTCGCCAACCAATTGTTCAATATGCGGTTCATCAGGTGCATTTGCTACAGAAAAACTGGTATGCGATTGGTGAAACGCCATTAAAAATAATTGTCGGCCCTGAATGTTATGCAATTGACCAATGATTAATTCACTTTTATCGTCCCATGA  >  NC_000913/3004536‑3005109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: