Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,233,262 | A→G | 9.4% | I534V (ATC→GTC) | fadH → | 2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,233,262 | 0 | A | G | 9.4% | 391.5 / 8.0 | 153 | I534V (ATC→GTC) | fadH | 2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked |
Reads supporting (aligned to +/- strand): ref base A (43/92); new base G (13/3); total (56/97) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.89e-04 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 |
Only 100 of 154 total aligned reads displayed.
GACGGGCGTGACGCTAAAACTCAATCACACCGTGACGGCGGATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATG‑ATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGACAATGTGGTGATCTGCGCAGGGCAAGAGCCAA > NC_000913/3232976‑3233550 | gACGGGCGTGACGCTAAAACTCAATCACACCGTGACGGCGGATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGAt < 1:865894/288‑1 (MQ=255) cGTGACGCTAAAACTCAATCACACCGTGACGGCGGATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTAGTTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCTACCAGCCAG‑AATATCG‑CCGAGTTCTGTAATGAATGGGGGATCGACAg < 2:166702/288‑1 (MQ=255) cGTGACGCTAAAACTCAATCACACCGTGACGGCGGATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGAACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAg < 1:71199/288‑1 (MQ=255) aaaaCTCAATCACACCGTGACGGCGGATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGAA‑AAGGCGACGTT‑TTTAAGGCCGCCGGGGGGATCCACCAGCCAG‑AAAATCG‑CCCGGGTCTGTAATGAATGGGGGGTCGGCAGTAGCCTacaa > 1:181361/1‑289 (MQ=255) cTCAATCACCCCGTGACGGCGATTCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑gg < 2:478207/289‑1 (MQ=255) ggtcagcggtgtataagagacgggTTACGGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGGGCCGCGCACTCCTCCAATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCt < 2:620872/265‑1 (MQ=255) aaTCACACCGTGACGGCGGATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGGTTGGTTTTTAT‑ACCGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAACCAG‑CATATCG‑CCGGGGTCTGTAATGAATGGGGGGTCGACAGTAAACTACAACA‑GGCtg > 1:478207/1‑289 (MQ=255) aaTCACACCGTGACGGCGGATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGGATGGTTTTTAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGGATCCAACAGCCAT‑AATAACG‑CCCGGTTTCGTAACTAATGGGGGGTCTACAGTTGccccaaaaaggctc > 2:87807/1‑277 (MQ=255) ggcggATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGGATGGTTTTGATAACGGGAAGGTT‑TTAAAGT‑AGGCGGGCGAAGCCACCAGCCAGAAATAT‑G‑CCGGGTTCTGTAATTAATGGGGGATCGCCAGGAGGCCACAACA‑GGCTGGTGGGGTAAAccc > 2:1259042/1‑288 (MQ=255) cggATCAGTTACCGGCTTTCGATGAAACGATCCTCGCCAGGGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCGCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGc < 2:451154/288‑1 (MQ=255) gtaTCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGGGCCGCGCACTCCGCCCATCGACGGGACCGATCGTCCGAAGGTATTGAGTTATCTCGGTGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCa < 1:969338/286‑1 (MQ=255) ggATCAGTTACAGGCTTTCGATGAAACGTGCCTCGCCAGTGGGATCGTGCCGCTCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AATATCT‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCa < 1:188740/288‑1 (MQ=255) ggATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCa < 2:788452/288‑1 (MQ=255) ggATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCa < 1:1254596/288‑1 (MQ=255) ggATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACCCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCAGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AATCTCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCa < 1:78887/288‑1 (MQ=255) ggctcgggttagtgtataagagacagATCCTCGCCAGTGGAGTCGTGCCGCGCACGCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAgg < 1:670361/263‑1 (MQ=255) ttACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAg < 1:28305/248‑1 (MQ=255) aCAGGCTTGCGATGAAACGAGCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAg < 1:309076/289‑1 (MQ=255) aGGCTTTCGATGAAACGAACCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGCTCGATCATCCGAAGGAATTGAGTTATCCCGATGTACTGCGCGACAAACCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGGTTGGTTTTCTT‑ACGGCGGTGTG‑GTTGAGTGAGACCGG‑GGATCACAAAGCCAC‑AAGCTAG‑GCCGGGGCTGTTATGAACGGGGGATCGGCAGTACTCCA‑AACACGTCTGGTGGCTTACGACAACAGGGAAGGccaaa > 2:384065/1‑284 (MQ=255) cTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTGATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATc < 1:774025/287‑1 (MQ=255) tCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGGTTGGGTTTGAT‑ACGGCGATGGA‑TTTAAGTTAGCCGGGCGAAGCCACCAACCAG‑AATATCG‑CCGGGGTCTGTAATGAATGGGGGGTCGACAGTAGCCTAAAACA‑GGCTGGTGGCTTaa > 1:620872/1‑264 (MQ=255) tCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGGATGGTTTTGGT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAAGCCACCAGCCCG‑AATATCG‑CCGGGTTCTTTAATGAATGGGGGAGCGGCGGTAACCTACAACA‑GGCTGGTTGCTTAAGCCCGCCGGGGATGCAgggccacc > 1:622809/1‑281 (MQ=255) tCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGCTTGGTGTTTGT‑ACGGCGCTTGA‑TTTTAATCAGGCGGGCGAATCAACCCGCCAG‑AGAAGCGACCGGGGT‑TGTAATGGAAGGGGGGACCACACTAGCCCCCAACA‑GGGCGCGGGCTTAAGCCCGCAGGGAATGCAG‑TAccccc > 2:224172/1‑288 (MQ=255) tCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGAT‑ACCGCGCAGTA‑TTTAAATCCGCCGGGCGAAACCACCAGCCAG‑AAAATCC‑CCGGGGTTTGTAAAGAATGGGGGATAGACAGTAACCTCCAAAA‑GCGTGGTGGCTTAATCCCGCAGGGGAGTAAG‑ATCCCCCg > 2:1218560/1‑289 (MQ=255) cggtCCTGGCCAGTGGGATCGTGCCGCCCACTCCGCCCATCGCCGGGATCGATCATCCGAAGGTATTGAGTTATCTCGAGGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACg < 2:1187254/286‑1 (MQ=255) tCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGAt < 1:1023169/289‑1 (MQ=255) tCGCCAGTGGACTCGTGCCGACCACTCGCCCCACGTGCGGGATCGATCATCCGAAGGTATTGAGTTTGCTCGAGGTACTGCGCGACAAAGCGCCGTTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGGTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTGCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGAt < 2:631499/289‑1 (MQ=255) cgggcACTCCGCCCATGGACGGGATCGGTCAACCGAAGGGATTGAGTTATGTGGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCaa < 2:1159428/286‑1 (MQ=255) gcgcACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCaaa < 1:1260135/289‑1 (MQ=255) gcgcACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGAT‑ACGGCGATGTA‑TTTAAATCAGCCCGGGGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGACAGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTTAACCCGCCGGGGACGCGA‑AACCCCCGTAGCACCCGGCAGGTCGTGATGCTCCAGCGCaca > 2:471791/1‑287 (MQ=255) aaGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGGATGCAG‑ATCCCCCGTAGCCCACGGCAGAATGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑ACAGGACAGGGGGTAGGCAAAacc > 1:958634/1‑288 (MQ=255) aGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAacca < 1:87807/288‑1 (MQ=255) gTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGa < 1:903863/238‑1 (MQ=255) aTTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGGATGGTTTTTAT‑ACGGCGATGTA‑TTTAAGTTACCCGGGCGAAACCACCAGCCAG‑AATATCG‑CCGGGTTTTGTAAAGAATGGGGGATCGGAAGGTGCCCAAAACA‑GGCTGGTGGCTTTAGGCCGCAGGGAATGCAA‑AACCCCAGCAGCCCCCGGCAGACTGTGAAGCTCCCGGGCCAAGCCAGGCAAA‑CAAGGACGGGGATAAGGAAAACCCCCCgc > 2:649055/1‑288 (MQ=255) ttGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCCCCAGCCCG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGACTAACACA‑AGGTGGTGGCGTAAGGCCGCCGGGAATGCCG‑ATCCCCCGTACCCCCCGGGAGATTGTGCATCCCCAGCGCAAAGCCA‑CCAAACCCAGGACAGGGG‑TAGGCAAAcccacccg > 2:549398/1‑285 (MQ=255) aTCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGCGGCGGGAGTGGATTTGAT‑ACGGCGGTGTT‑ATTTAGTCAGCCGGGGGGATCCCCCAGCCAA‑AATATCC‑CCGGGGTCTTGTATGAAAGGGGGGACGACAGTAGCCTCCAACA‑GACTGGTGGTGTAAGGCTGCAGGGAATGCAG‑AAACCCCGTAGCCCACGGCAGGTGGGGATACTCCAGCGCCACGCCA‑GCAAA‑CCCGGAATAGCGTAAGGCCCAACCACCGGCgggccccc > 2:629882/1‑284 (MQ=255) tctcGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGGTCGACAGTAGCCTAAAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCCCGGCAGATTGTGATGCCTCCGCGCAAAGCCA‑GCAAA‑CCAGGAAAGGGGGTAGGCAAAAACAACGGCTGGATCCa > 1:1392372/1‑288 (MQ=255) gATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGca < 1:1094272/289‑1 (MQ=255) gATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGca < 1:115415/289‑1 (MQ=255) aCTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCaccacc < 1:997213/289‑1 (MQ=255) gcgcGACAAAGCGCCGGTTGTCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTg < 1:10845/289‑1 (MQ=255) gcgcGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGAATGGTTTTGGT‑TTCGCGGTGTATTTTAAGCACACCGGCCGAA‑CCACCCGCCAG‑CATAATG‑CCGGGTTGTGTCCATAATTGGGGATCGACCGTAACCCACAACA‑GGCTGGCGGGCTAAGCCCCCAGGGGATGCAGAAACCCCCGCTG‑CCAAGGCCGGTTGGGTTTTCCCAGCGCAAAGCCA‑GGCAA‑CCCAGACAGGGGGTAGGCCAAACCCCCGGCCGGGACCCCCGCCCCACCCt > 2:485672/1‑288 (MQ=255) cgcgACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGGTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGGCCGCAGGGGATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGACA‑GCAAA‑CCAGGACAGGGGGTAGGCAAAACCACCGGCTGGATCCATCCCACCAccccg > 1:119689/1‑286 (MQ=255) cgcgACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AAAATCG‑CCGGGGTCTGTTATGAAAGGGGGGTCGACAGTCACCTACAACC‑GGCTGGTTGGTTAAGCCCGCAGGGTATGCAG‑CACCCCCGTAACCCACGGCCGCTTGGGGTGCTCCCGCGCCACACCA‑CCAAA‑ACACGGCAGGGGGTGGGCAAAACCACCGGATgggccactcccaccaacccg > 2:115415/1‑270 (MQ=255) cAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGGATGGTTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AATAACG‑CCGGGTTCTGTAAGGAATGGGGGAACGACAGTAGCCTCAAAAA‑GGCGGGGGGGTTAAGCCGGGGGGGAAGGAAG‑TACCCCCGAACCCCACGGGCGGTGTGGATGCCCCGCGGCAAAGCCA‑CACAA‑CCGGGAAAGGGGTAGGGACAAACACCCGCCGGGACCCCACCCACACCCCTGCtctc > 2:1362078/1‑288 (MQ=255) aaGGGCCGTTTGGCAACAACGTGGCCATCATCGGTTGTGGCGGGATTGGTTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGACGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGGTCGACAGTAGCCTACGACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGGGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTcgcg < 2:639624/289‑1 (MQ=255) aaGCGCCGGTTGGCAACAAAGTTGCTATCATCGGTTGTGGCGGGATTGGTTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTcgcg < 1:1289928/289‑1 (MQ=255) aaGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTcgcg < 1:1192882/289‑1 (MQ=255) gTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGtgtg < 1:471791/288‑1 (MQ=255) aaaGTTGCCATCATCGTGTGGTGAGTGTTTGGTTTTGGT‑AAGGCGATGTA‑TTTAAGGCAGCCGGGGGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAAGGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCGACGGCAGATTGGGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATG‑ATcc < 2:246582/289‑1 (MQ=255) gTTGCGAGCATCGTTTGTCGCGGGATTGGTTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATG‑ATc < 2:1369467/285‑1 (MQ=255) catcatCGGTTGTGGCGGGATTGGTTTTGAT‑GCGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATG‑ATCCCAGGCGt < 1:85672/288‑1 (MQ=255) catcGGTTGTGGCGGGATTGGTTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGGTCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATG‑ATCCCAGGCGTAAGt < 1:649055/289‑1 (MQ=255) tggggCGGGAGTGGTTTTGAT‑ACGGCGAGGTA‑TTTAAGTCAGCCGAGCGAATCCACCAGCCAG‑AATATCG‑CCGGTTTCTGTAATGAATGGGGAATCGACAGTAGCCTACAAGA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATG‑ATCCCAGGCGTAAGTTATCaga < 1:241601/286‑1 (MQ=255) gCGGGAGTGGGTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAAACCACCAGCCCG‑AATATCG‑CCGGGGTCTGTAATGAATGGGGGGTCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCCCGGCAGATTGTGATGCTCCCGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCCGCTGGATCCCTCCCACCCCCCTGCCCTCGCGGGGGGGGCAAATGCATCCC‑GGCGGAAGTTATCAGCAGAt > 2:50245/1‑289 (MQ=255) ggTTTTGAT‑ACGGCGATGTA‑TGTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATG‑ATCCCAGGCGTAAGTTATCAGAAGATTGACGATg < 1:485672/289‑1 (MQ=255) ttttGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATG‑ATCCCAGGCGTAAGTTATCAGAAGATTGACGATGa < 1:549398/288‑1 (MQ=255) ttttGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGAAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATG‑ATCCCAGGCGTAAGTTATCAGAAGATTAACGATGa < 1:33063/288‑1 (MQ=255) tttgGAT‑ACGGGGTTGGA‑TTTGAGTCAGGCGGGGGAACCGACAAGGCCG‑AAGATCG‑CCGGGTTCTGTAATGAATGGGGGGTCGACAGTAGCCTACAACA‑TGCTCGTGGCTTAGGCACGCAGGGAATGCCG‑GTCCCCCGTGGCCCACGGCAGATTGTGATGCGCCATCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCGTCGTACCACCCTGCTCTCGCGGGGTGTGAAAATG‑ACCCCAGGCGTAAGTTATCAGAAGATTGACGATGa < 2:1030137/288‑1 (MQ=255) tGAT‑ACGGGGAGGTA‑TTTAAGTCAGCCTGGCGAATCCTCCAGCCAT‑AATATCG‑CCCGGTTCGGTAATGAATGGGGGATCGACAGTAGCCTACACCA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCGG‑ACCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATG‑ATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACggg < 2:173075/289‑1 (MQ=255) gaaT‑ACGGCGATGTG‑TTTAAATCAGCCTGGCGAATCAGCCAGCCAG‑AATATGG‑CCGGGTTCTGTAATTAATGGGCGAGCGACAGGAGCCGACGACA‑GGCTTGTGGCTTAAGCCCGCAGGCGATGCGG‑CTCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCACGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCGCCCTGCTCTCGCGGGGTGTGAAAATG‑ATCTCAGGCGTAAGTTATCAGAAGATTGACGTTGACggg < 2:974747/287‑1 (MQ=255) gTCAGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGGTCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATG‑ATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATgtggtg < 1:597474/281‑1 (MQ=255) gTCAGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGGTCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATG‑ATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATgtggtg > 2:597474/1‑281 (MQ=255) cAGGCGGGCGAAACCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATG‑ATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCg < 1:1218560/289‑1 (MQ=255) aGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCaaa > 2:87475/1‑155 (MQ=255) aGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCaaa < 1:87475/155‑1 (MQ=255) aGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTcg < 1:199737/212‑1 (MQ=255) aGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTcg > 2:199737/1‑212 (MQ=255) aGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCggg > 1:397348/1‑216 (MQ=255) aGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCggg < 2:397348/216‑1 (MQ=255) aGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATG‑ATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACggg > 2:1243065/1‑266 (MQ=255) aGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATG‑ATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACggg > 2:804894/1‑266 (MQ=255) aGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATG‑ATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACggg < 1:804894/266‑1 (MQ=255) aGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATG‑ATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACggg < 1:1243065/266‑1 (MQ=255) aGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATG‑ATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGa > 1:817621/1‑289 (MQ=255) ccGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGca < 1:683354/196‑1 (MQ=255) ccGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGca > 2:683354/1‑196 (MQ=255) gggCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGACTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATG‑ATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATAAACGGCGAAACg < 1:1033451/289‑1 (MQ=255) gggCGAAGCCACCAGCCGG‑AATATCG‑CCGGGTTCTGTAAGGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATG‑ATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACg < 2:958634/289‑1 (MQ=255) cGAAACCACCAGCCAG‑AATATGG‑CCGGGATCTGTAATGAATGGGGGAGCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATG‑ATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCa < 2:1020030/288‑1 (MQ=255) tCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATG‑ATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCAGGTAt < 1:358644/289‑1 (MQ=255) cagccag‑AATATGG‑CCGGGTTCTGTAATGAATGGGGGTTCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCAATCGCACCACCCTGCGCTCGCGGGGTGTGAAAATG‑ATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCa < 2:1067349/289‑1 (MQ=255) cagccag‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATG‑ATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGGTGTGATCCACGGCGAAAccgcggtactcgca > 1:670213/1‑276 (MQ=255) ccag‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATG‑ATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCAGGTATTAg > 1:487554/1‑284 (MQ=255) aaTATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGATGGCGTAAGCTCGCAGGGAATGCAG‑ATCCCCCGTAGACCACGGCAGATTGTGAGGCTCCAGCGCAAAGCCA‑GCAAA‑ACAGGACACGGGTTAGGCAAAACCAACGGCACGATCCACCGCACCACCCTGTTCTCGAGGGGTGTGAAAATG‑ATCCTAGACGCCAGTTCTAACACGATTCGAGATGCCGGCCTGAGTCCGGTGATCAACGGCGAAACGCCAGTACCAGCTGTCGACa > 2:41245/1‑289 (MQ=255) aaTAACG‑CCGGGTTCGGTAATGAAGGGGGGATCGACAGTAGCCGACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATG‑ATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGACa < 2:1254519/289‑1 (MQ=255) atatCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCATACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATG‑ATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGACa < 1:697112/288‑1 (MQ=255) atatCG‑CCGGGGTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGACCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATG‑ATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGt < 1:968696/283‑1 (MQ=255) ggTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATG‑ATCCAAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCACCGGCGAAACGCAGGTATTAGCAGGGGACACTGTGGTGAt > 2:1092367/1‑289 (MQ=255) tAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAAGGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATG‑ATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGa < 1:918517/270‑1 (MQ=255) gaatgGGGGTGCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCATATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTTGATCCATCGCACCAACCTGCTCTCGCGGGGTGTGAAAATG‑ATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGACAATGTGGTGATCTGCGCAGGGc < 1:218317/289‑1 (MQ=255) gaatgGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATG‑ATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGACAATGTGGTGATCTGCGCAGGGc < 1:993449/289‑1 (MQ=255) aatgGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAACATG‑ATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGACAATGTGGTGATCTGCGCAGGGCa > 2:486823/1‑289 (MQ=255) aatgGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATG‑ATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACg < 2:1167314/248‑1 (MQ=255) aatgGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATG‑ATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACg > 1:1167314/1‑248 (MQ=255) aatgGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATG‑ATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACg > 1:1160704/1‑248 (MQ=255) aatgGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCGCTCGCGGGGTGTGAAAATG‑ATCCCAGGCGTAAGTTATCAGAAGATTGACGCTGACGGGCTGCCTGTGGTGTTCAACGGCGAAACAGACGTATTAGCAGCTGACATTGTGGTTCTCTCCGCAGGTCa > 1:113370/1‑289 (MQ=255) gggATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCCCCACCCTGCTCTCGCGGGGTGTGAAAATG‑ATCCCAGGCGTAAGTTCTCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAAACGCGAAACGCAGGTATTAGCAGTGGCAAATgtggtcactgtctcttatcacca > 2:918517/1‑269 (MQ=255) gggATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATG‑ATccc > 1:640608/1‑182 (MQ=255) aTCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATG‑ATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGACAATGTGGTGATCTGCGCAGGGCAAGAGCCaa > 2:1185849/1‑289 (MQ=255) | GACGGGCGTGACGCTAAAACTCAATCACACCGTGACGGCGGATCAGTTACAGGCTTTCGATGAAACGATCCTCGCCAGTGGGATCGTGCCGCGCACTCCGCCCATCGACGGGATCGATCATCCGAAGGTATTGAGTTATCTCGATGTACTGCGCGACAAAGCGCCGGTTGGCAACAAAGTTGCCATCATCGGTTGTGGCGGGATTGGTTTTGAT‑ACGGCGATGTA‑TTTAAGTCAGCCGGGCGAATCCACCAGCCAG‑AATATCG‑CCGGGTTCTGTAATGAATGGGGGATCGACAGTAGCCTACAACA‑GGCTGGTGGCTTAAGCCCGCAGGGAATGCAG‑ATCCCCCGTAGCCCACGGCAGATTGTGATGCTCCAGCGCAAAGCCA‑GCAAA‑CCAGGACAGGGGTTAGGCAAAACCACCGGCTGGATCCATCGCACCACCCTGCTCTCGCGGGGTGTGAAAATG‑ATCCCAGGCGTAAGTTATCAGAAGATTGACGATGACGGGCTGCATGTGGTGATCAACGGCGAAACGCAGGTATTAGCAGTGGACAATGTGGTGATCTGCGCAGGGCAAGAGCCAA > NC_000913/3232976‑3233550 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |