Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,296,154	T→C	10.6%	intergenic (+361/+283)	gltP → / ← yjcO	glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,296,154	0	T	C	10.6%	70.6 / 3.6	43	intergenic (+361/+283)	gltP/yjcO	glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base T (19/19);  new base C (2/3);  total (21/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.23e-01

CGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGAT‑GCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCC  >  NC_000913/4295870‑4296432                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                       cGCCAGACAGGGGAATAGCCTGATTTAGCGTGATTTTGTAGGGCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGAT‑GCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCg                                                                                                                                                                                                                                                                                    <  1:568277/289‑1 (MQ=21) atccAGACAGCGAAATAGCTTGATTGAGTGTGATTTTGTAGGTCGAGTAAGGCTTTTATGCCGCATCCAACATCAACGCCGGCT‑GGGACGCTTAACGCGTCTTATCAGGCCTACGCCAAACAGCTCAATAGCCTAATTTAGCGTGATTTTGTAGGGCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGAAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCg                                                                                                                                                                                                                                                                                    <  1:1004306/287‑1 (MQ=11)     gaacatcagattAGCCTGATTTGTCGATATTTTTTGGGTAGGATAAGCGGTTTATGCCGCGTCGGGAATGACGGCTTGAT‑GCGACGCTAAACGCGTCTTATCAGACCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATc                                                                                                                                                                                                                                                                                <  2:1377597/278‑1 (MQ=11)            gcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGAT‑GCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGCTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATc                                                                                                                                                                                                                                                                         >  2:310880/1‑289 (MQ=21)                tAGCCTGATGTAGCGTGATTTTATAGGACGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGAT‑GCGACGCTTAACGAGTCTTATCAGGCCTAGGCCAGACAGCGCAATATCCTGATTTCGCGTGAATTTGTAGGACGGATAAGGAGTTTACGCCCCATCCGACATCAACTCCTGATCCGACGCTGACAGTGTCATATCAGGCCAATGCCAGCCAGTGCAATACCCTGCTTACCCGTGATTTGGAACAGCGGGTACGGTGATTATGCCGCAGCCGACA‑CAACg                                                                                                                                                                                                                                                                     >  2:1053383/1‑288 (MQ=2)                   ccTGCTTTCGCGTGATTTTTTAGTTTGGATAAGGGGTTGATGCCCGATCAGACATCAACGCCTGAT‑GCGAGGCTTACCGCGTCGTATCAGGCCTACGCCAGAAAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGTATAAGGCGTTTCCGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGcc                                                                                                                                                                                                                                                                   <  2:297177/288‑1 (MQ=11)                     tGATTTAGCGTGCTTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGAT‑GCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAAGAGCCTGATTTCGCGTGCTTTTGTAGGTCGGATAACGCGTTTACGCCGCATCAGACATCAACGCCTGATGCGACGCTTTCCGCGTCTTATCACGCCTACGCCAGCCAGCGCCATAGCCTGATTTAGCCTGTAGTTGTAGGTCGGATACGGCGTTTATGTCGCATCTAACATCCACGCCCGa                                                                                                                                                                                                                                                                >  2:726995/1‑289 (MQ=11)                      gATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGAT‑GCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAATTAGCGTCATTTTGTAGGTCTGATAAGGCGTTTATGCCTCATCCGACATCAACGCCTGAt                                                                                                                                                                                                                                                               >  1:1373926/1‑289 (MQ=25)                       gttaggcttaagttgttgggtggtaTACGGCGTTTATGGCGGGTCCGACGTCAACGCCCGAG‑GCGACGCTTAACGCGTCTTATCAGGCCGACGCCAGAAAGCGCAATAGTCTAATTTAGCGTGAGTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCGGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATg                                                                                                                                                                                                                                                              <  2:1171903/265‑1 (MQ=11)                       aTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGAT‑GCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGCCGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAATTAGCTGGATTTTGTCGATCGGATCATGGCTTCATGCCCCAGTCCACCTCAACGCCTTAt                                                                                                                                                                                                                                                               >  2:1087948/1‑288 (MQ=11)                        tttAGTGTGATTTTGGAGGTCGGATAAGGCGTTTCTGCCGCATCGAACATGAACGCCTGAT‑GCTACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGAGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGGTAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGc                                                                                                                                                                                                                                                             <  2:996537/289‑1 (MQ=255)                                  ttttGTAGGTCGAATAGGGCGTTTAGGCCGCATCCACCACCAACGCC‑GATGGCGACGCTTAACGCGTTTTATCAGGCCTAGGCCAGACAGCGCAATAGCCTTATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTGACGCCGCATCCGACATCAACGCCGGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTaa                                                                                                                                                                                                                                                    <  1:697192/288‑1 (MQ=11)                                                tAAGGCGTTTATGCCGCATCCGACATCAACGCCTGAT‑GCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGc                                                                                                                                                                                                                                     <  1:720260/289‑1 (MQ=37)                                                tAAGACGTTTCTGCCGCATCCGACATCATCGCCTGAT‑GCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCTTGATTTAGCGTGATTTTGTAGGTCGGAGAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGc                                                                                                                                                                                                                                     <  1:754759/289‑1 (MQ=255)                                                gatggCGTTTACTCCGCGTCAGACACCACGGCCGGAT‑GCTACGATTGACGCGTCTTATCAGGCCTACGCCAGACAGCGAAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGc                                                                                                                                                                                                                                     <  2:1177963/286‑1 (MQ=11)                                                        ttATGCCGCATCCGACATCAACGCCTGAT‑GCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCCAATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCa                                                                                                                                                                                                                             >  1:1341586/1‑289 (MQ=37)                                                         tATGCCGCATCCGACATCAACGCCTGAT‑GCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAg                                                                                                                                                                                                                            >  1:898770/1‑289 (MQ=37)                                                         tATGCCGCATCCGACATCAACGCCTGAT‑GCGACGCATAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCTTGATGCGACGCTTGCAGCGTCTTATCAGGCCTACGCCATCCAGCGCTATTGCCTGATTTAGCGTGATTTCGTATGTCGGCCAATGCGTTTCTGCCGCCTCATACATCTATGCAGGTTCCGACGCTTACCGCGTCTTATccgcccgacctcac                                                                                                                                                                                                                            >  2:1032153/1‑276 (MQ=11)                                                          atgcccgcgaggaaatcgcgacttGAG‑GCGAAGCTTAAGGCGTCTTATCAGGCCTACGCTGGACGGAGCAATGTCCTGATTTAGCGTGATTTTGTAGGTCGGATGAGGCGTTTCCGCCGCATCCGACATAAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGa                                                                                                                                                                                                                           <  2:1078179/266‑1 (MQ=11)                                                          aTGCCGCATCCGACATCAACGCCTGAT‑GCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGa                                                                                                                                                                                                                           <  1:310880/289‑1 (MQ=37)                                                                cATCCGACATCAACGCCTGAT‑GCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAgcg                                                                                                                                                                                                                      >  2:752269/1‑288 (MQ=35)                                                                                 tGAT‑GCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCg                                                                                                                                                                                                    <  1:752269/289‑1 (MQ=35)                                                                                                     tctgtaagggcttaagacAGACAGTGCAGTAGCTGTGTTCAGAGTGAATTTGTGGGTCGTGTAAGGTGATTACGCCGGAAACTAGAGAAATGCCGGATGTGAGGCTTGCCGCGTCTTATAAGGCAGACGCCAGACAGCGCAGTGGCCTGATTTAGCGTGATTTTGTAGGTCGCATAAGGCGTTTATGCCGCAGCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAt                                                                                                                                                                                  <  2:123442/271‑1 (MQ=2)                                                                                                     tCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATa                                                                                                                                                                                 >  2:1129741/1‑289 (MQ=33)                                                                                                      tttaccgggCCTACGCCAGCCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCACCTCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAACGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATaa                                                                                                                                                                                <  1:103884/282‑1 (MQ=16)                                                                                                              gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTCGCGTCATTTTGTAGGTCGGATAATGCGTTTATGCCGAATCCGACATCAACGTCTGCTTCGACGCTTACCGCGTCTTATCAGGCCTACCACATACAGCGCAACAGCCTGATTAAGCGTGACTTTGTAGGCCGGACACGGCGttca                                                                                                                                                                        >  2:86851/1‑287 (MQ=11)                                                                                                                   cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGc                                                                                                                                                                                                                                     <  1:571128/223‑1 (MQ=34)                                                                                                                   cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGc                                                                                                                                                                                                                                     >  2:571128/1‑223 (MQ=34)                                                                                                                        gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTCGCGTGATTTTGTAGGTCGGATAAGACGTTTATGCCGCATCCGACCTCACCGCCTGATGCGACGCTTAACGCGTCTTATCAGTCCTCCGCCAGCCAGCGCAACAACCTTATTTACCTTGATTTTGTAGGTCGAATCAGTCGttacctctccact                                                                                                                                                               >  2:347342/1‑277 (MQ=11)                                                                                                                        gACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCAGCATCCGACATCAACGCCTGATGCGACGCATGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTCTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGATAGCGCAATAGACTGATTTAGCTTGATTTTGTAGGTCGGATAAGGCGTTTCACCCGCATcc                                                                                                                                                              >  1:1073941/1‑289 (MQ=255)                                                                                                                             cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTATGCCAGACAGCGCAATATCCTTATTTCGCGTGATTTTGTAGGTCGGATAACGccgttaccccccacccgcca                                                                                                                                                          >  2:880979/1‑269 (MQ=11)                                                                                                                             cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCATACAGCGCAATAGCCTGCTTTCGCGTTATTCTGTAGATCGGATACGGCGTTTAGTCTGCATGCGACa                                                                                                                                                          >  2:1040884/1‑288 (MQ=11)                                                                                                                                        gaataaGCGTGACTTTGTAGGGCGAATAAGGCGTTTACGCCGCAGACGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATACTAGACATCGCAATCGCCTGATTTAGCGTGATGGCGTAGGTCGGATACTGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACTCTTAACGCGTCTTACCAGGCTTACGCAAGACAGAGAAATAGCCTGACTTAGCGTTCGTTTGTATGTCGGTTAACGCGTTTAGGCCGccttagtcacgcatgcctg                                                                                                                                                >  2:1161881/6‑269 (MQ=11)                                                                                                                                                                           tACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAgg                                                                                                                                                                                        >  1:369145/1‑212 (MQ=34)                                                                                                                                                                           tACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAgg                                                                                                                                                                                        <  2:369145/212‑1 (MQ=34)                                                                                                                                                                                                                       cTTATCAGGTCTACGCCAGACAGCGCAATGGCCTGTTTTAGCGGGATTTGTTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATAAACGCCGGATGGGACGCTTGACGCGGCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATaa                                                               <  2:461087/289‑1 (MQ=11)                                                                                                                                                                                                                                                                    aTTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGCCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGTTCCGCCGCTTACGGCGTCTTATCAGGCCTACCCAAGACAGCGCATTAGCCTGTTTTACCTTGATTTTGTAGGTTGGATAAGGCGTTTCCCCCGCTTCAGCACTCaatgccgtatgctcgcttgg                   <  2:465844‑M2/288‑21 (MQ=255)                                                                                                                                                                                                                                                                          tAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCaatgcctgatgcgacgcttgccgcg              <  1:652853‑M2/287‑26 (MQ=255)                                                                                                                                                                                                                                                                               cGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCaatgcctgatgcgacgcttgccgcgtcttatc       <  1:202112‑M2/289‑33 (MQ=255)                                                                                                                                                                                                                                                                               cGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACCTCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGCCAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCCGATACGGCGTTTACGCCCCATCCGACCTCcatgcctgctgctacgcttgccgcgtcttat        <  2:584156‑M2/288‑32 (MQ=255)                                                                                                                                                                                                                                                                                   tAAGGCGTTTACGCCGCAGCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCaatgcctgatgcgacgcttgccgcgtcttatcaggc   >  1:264169‑M2/1‑253 (MQ=255)                                                                                                                                                                                                                                                                                    aaGGAGGTTCTGGCGCTTGCGACGTCAGCGCCTGATGTGACGCTTGCTGCGTCTTATCAGGCCTACGCCAGACAGCGCAAGACCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGGCATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCaatgcctgatgcgacgcttgccgcgtcttatcaggc   >  2:674866‑M2/1‑252 (MQ=255)                                                                                                                                                                                                                                                                                     aGGCGTGTTCGCCGCATCCTGCATCAACGCTTGATGCGAGGCTTGCCGCGTCTTATCAGGCCTGCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCaatgcctgatgcgacgcttgccgcgtcttatcaggcc  >  2:896062‑M2/1‑251 (MQ=255)                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                       CGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGAT‑GCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCC  >  NC_000913/4295870‑4296432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑