BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	75,042	T→C	3.8%	S147G (AGC→GGC)	thiB ←	thiamine/thiamine pyrophosphate/thiamine monophosphate ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	75,042	0	T	C	3.8%	364.5 / 2.7	112	S147G (AGC→GGC)	thiB	thiamine/thiamine pyrophosphate/thiamine monophosphate ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand): ref base T (60/46); new base C (3/2); total (63/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.45e-01

Only 100 of 120 total aligned reads displayed.

CAGATAGTGACCTTCGCTGAAGTTCGCGGCGGCGTAGTTATCTTTCTTCTCTTCGAGAATGTGATAAGCCGGAGAGGTGGTGTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACATCGGCTTTACTGTTTTTGCCTTCCATCCGTAGACGGTTGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGTT  >  NC_000913/74755‑75329

cAGATAGTGACCTTCGCTGAAGTGCGCGGTGGCTGAGTTATGTTTCTTCTCTGCGAGAATGTGATAAGCCGGAGAGGGGGGGTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATGGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGctc                                                                                                                                                                                                                                                                                                  <  2:1068180/289‑1 (MQ=255)

         aCCTTCGCTGAGGTTCGCGGCGGCGTAGTTATCTTTCTTCTCTTCGGTAATGTGATAAGCCGGGGAGGTGGTGTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAg                                                                                                                                                                                                                                                                                          <  2:1543339/288‑1 (MQ=255)

         aCCTTCGCTGAAGTTCGCGGCGGCGTAGTTATCTTTCTTCTCTTCGAGAATGTGATAAGCCGGAGAGGTGGTGTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGt                                                                                                                                                                                                                                                                                         >  1:835309/1‑289 (MQ=255)

           cTTCGCTGAAGTTCGCGGCGGCGTAGTTATCTTTCTTCTCTTCGAGAATGTGATAAGCCGGAGAGGTGGTGTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGt                                                                                                                                                                                                                                                                                         >  2:1502799/1‑287 (MQ=255)

             tCGCTGAAGTTCGCGGCGGCGTAGTTATCTTTCTTCTCTTCGAGAATGTGATAAGCCGGAGAGGTGGTGTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCtt                                                                                                                                                                                                                                                                                     >  1:565002/1‑289 (MQ=255)

             tCGCTGAAGTTCGCGGCGGCGTAGTTATCTTTCTTCTCTTCGAGAATGTGATAAGCCGGAGAGGTGGTGTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTCATCGCTCTCAACCAGTTCtt                                                                                                                                                                                                                                                                                     >  2:353198/1‑289 (MQ=255)

              tgCTGACGTTCGCGGCGGCGCAGTTATCTTTCTTCCCTTCGAGAATGTGATATGCCGGAGAGGTGGTGTAACTCAGTATCTGATCGCTTTCACTATTTAAAAACAGGCCGTAGGCTTCGCTCCAGCTTTTGGTGACCGTGACCGTTATCGTCGCCAGTTTCTGCCAGGCTTGTGGGTTGTCATCGCCATCGGCTTTTCGCATACATAGCACCAGACCAAGCCCAGGTTTCCTTGCTCGACGATCCTGACGAATCACCCCCCCGTTTTTATCGCTCTCTATCTGTTCtt                                                                                                                                                                                                                                                                                     >  2:1232320/2‑288 (MQ=255)

              cGCTGAAGTTCGCGGCGGCGTAGTTATCTTTCTTCTCTTCGAGAATGTGATAAGCCGGAGAGGTGGTGTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCCCTCAACCAGTTCtt                                                                                                                                                                                                                                                                                     >  1:778612/1‑288 (MQ=255)

               gCTGAAGTTCGCGGCGGCGTAGTTATCTTTCTTCTCTTCGAGAATGTGATAAGTCGGAGAGGTGGTGTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCACCCCCGGTCTCCTGGTTCGCGGATCCTGATAAATCACCCGCCCGTCTTGCTCGCCCCCAACCAgttcgttc                                                                                                                                                                                                                                                                                   >  2:1574848/1‑289 (MQ=255)

                 tGAAGTTCGCGGCGGCGTAGTTATCTTTCTTCTCTTCGAGAATGTGATAAGCCGGAGAGGTGGTGTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATAACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAg                                                                                                                                                                                                                                                                                 >  1:1301605/1‑289 (MQ=255)

                        cgcgGCGGCGTAGTTATCTTTCTTCTCTTCGAGAATGTGATAAGCCGGAGAGGTGGTGTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGACAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTtgt                                                                                                                                                                                                                                                                          >  1:1014667/1‑289 (MQ=255)

                            gcggcgTAGTTATCTTTCTTCACTTAGAGCATGTGATAAACCGGAGGGGTGGTGTAACTCAGTACCAGTTCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGATTGTGGGGCGTCAGCGCCATAGACGTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGc                                                                                                                                                                                                                                                                       <  1:619790/288‑1 (MQ=255)

                               gcgTAGTTATCTTTCTTCTCTTCGAGAATGTGATAAGCCGGAGAGGTGGTGTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGt                                                                                                                                                                                                                                                                   >  1:1507381/1‑289 (MQ=255)

                               gcgTAGTTATCTTTCTTCTCTTCGAGAATGTGATAAGCCGGAGAGGTGGTGTAACTCAGTACAAGATCGCTTTCACCTTTTAAAAACAGGCGGTAGGCTTTGCTCCCGCCTTAGGTGACCGTGACCGTTTTCTTCGCCAGCTTCTGCCCGGCTTGTGGGGCGTCATCGCCATAGACTTTTGGCATCCATAGCAACAGCCCACGCCCCGGTGTACTGGTTCGCGGCTCCTGATAAAGCACCCTCCAGTTTTGATTGCTCTCAGCCAGTTCGTTCAGGCTTTGTGGCGGGt                                                                                                                                                                                                                                                                   >  2:1446907/1‑289 (MQ=255)

                                       aTCTTTCTTGTCTTCGAGAATGTGATGAGCCGGAGAGGTGGTTTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCATCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGt                                                                                                                                                                                                                                                           <  2:842689/289‑1 (MQ=255)

                                         ctttcttCTCTTCGAGCATGTGATAAGCCGGAGAGGTGGTGTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCCTAGCAACAGACCCAGCCCCGGTGTACTCGTGCGCCGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCACCCAGTTCTTTCCGGCTTTGTGGCGGGTTTTTCCGttt                                                                                                                                                                                                                                                         >  1:654503/1‑289 (MQ=255)

                                               tctcttcGAGAATGTGATAAGCCGGAGAGGTGGTGTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCtt                                                                                                                                                                                                                                                   >  1:761164/1‑289 (MQ=255)

                                                   ttcGAGAATGTGATAAGCCGGAGAGGTGGTGTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCACCAGTTTTGATCGCTCTCAACCAGGTCTTTCAGGTTTTGTGGCCGGTTTTTCAGGTTGTTTTTGTCa                                                                                                                                                                                                                                               >  1:1003156/1‑289 (MQ=255)

                                                    tcGAGAATGTGATAAGCCGGAGAGGTGGTGTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGct                                                                                                                                                                                                                                                                                                   <  2:1074069/236‑1 (MQ=255)

                                                                 agggcGGAGAGGGTGTGTAACTCAGTACCAGATCGCTTTCACCTTTTAAAACCAGGCCGTAGGCTTCGCTCCAGCCGTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCACGCGTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGTTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGaa                                                                                                                                                                                                                                <  2:173888/285‑1 (MQ=255)

                                                                 aaGCCGGAGAGGTGGTGTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGaa                                                                                                                                                                                                                                <  1:1040759/289‑1 (MQ=255)

                                                                       gagaGGTGGTGTAACTCAGTACCAGATCGCTTTCACCTTTTACAACCTGTCCGTAGGGTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGc                                                                                                                                                                                                                           <  2:573935/288‑1 (MQ=255)

                                                                       gagaGGTGGTGTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCCCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAg‑g                                                                                                                                                                                                                                    <  1:669341/279‑1 (MQ=255)

                                                                        tataaggtacagccttcgTACCAGATCGCTTTCACCTTTTAAAACCTGGCCGGAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCATTTTCTGCCAGGCTTGTGTGGCGTCATCGCCATAGACTTTTTGCATCCATAGCCACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑a                                                                                                                                                                                                                        <  2:1106592/272‑1 (MQ=255)

                                                                                 gtaactagttACCAGGTCGCTTTAGCCTGTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCGGCCAGGCTTGTGGGGCGTCATAGCCATAGACTTGTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCCTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCaaa                                                                                                                                                                                                                <  2:654503/279‑1 (MQ=255)

                                                                                 gtAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCaaa                                                                                                                                                                                                                <  2:565002/288‑1 (MQ=255)

                                                                                 gtAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCCGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCaaa                                                                                                                                                                                                                <  1:988566/288‑1 (MQ=255)

                                                                                          tACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTCCGTTTGTTCTTGTCATTAACGAAG‑GCGAAGTAGGC‑AATAACAAAAGGTAAGaaa                                                                                                                                                                                                      >  2:782473/1‑289 (MQ=255)

                                                                                           aCCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGa                                                                                                                                                                                                        <  1:782473/286‑1 (MQ=255)

                                                                                            ccAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAgt                                                                                                                                                                                                    <  2:276061/289‑1 (MQ=255)

                                                                                               gATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCATCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTGTCAGGCTTTGTGGCGGTGTTTTGATATTGTTCTCTGCA‑AAACCACGAGCGAACTAGCG‑TTAACCTAACGGTACGACAGTGTc                                                                                                                                                                                                 >  2:1395965/1‑289 (MQ=255)

                                                                                               gATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTc                                                                                                                                                                                                 >  2:337467/1‑289 (MQ=255)

                                                                                               gATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGGTTGTTCCTGTCATAAACGGAG‑GCGAAGTAGCCAATAAT‑AAACggctgtctcttaaa                                                                                                                                                                                                 >  1:1106592/1‑277 (MQ=255)

                                                                                                aTCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTTATAAACGAAG‑GCGAATGAGCC‑ATAATCCAACGGTACGAAAGGGTCa                                                                                                                                                                                                >  2:1026472/1‑289 (MQ=255)

                                                                                                    cTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCattat                                                                                                                                                                                            >  1:1103832/1‑289 (MQ=255)

                                                                                                               tAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GGCAAGTAGCC‑ATAATCAAACGGTAAGAAAGTTGCATTATTCCAGccgtcg                                                                                                                                                                                 >  2:21375/1‑289 (MQ=255)

                                                                                                                 aaaaCAGCCCGTAGGCTTCTGTCCAGCCTTTGGTGACCGGTACCGTTTTCTCCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTGTTTCAGTTTGTTCTTTTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCggg                                                                                                                                                                               <  2:176036/289‑1 (MQ=255)

                                                                                                                       ggCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAg                                                                                                                                                                                                                               >  1:198368/1‑236 (MQ=255)

                                                                                                                        gCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCa                                                                                                                                                                                                                  <  1:942328/247‑1 (MQ=255)

                                                                                                                        gCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCa                                                                                                                                                                                                                  >  2:942328/1‑247 (MQ=255)

                                                                                                                        gCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGATCTCAACCAGTTCTTTCAGCCTTTGTGGCGGGTTTTTTAGTTTTTTCTTTTCTTAAACGAAG‑GTGAAGTAGCC‑ATAATCAAACTGTTAGGAAGTGGCAATATTTCAANCGCCGGGGACCTTa                                                                                                                                                                        >  2:1161938/1‑289 (MQ=255)

                                                                                                                        gCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGACCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCACTTTGTTCTTGTCATCAACGAAG‑GCGAAGTAGAC‑ATAATCCAACGGTACGGAAGTGTCCTTATTCCAGCCGTCGGGCCCGTTa                                                                                                                                                                        >  2:1090432/1‑289 (MQ=255)

                                                                                                                               gCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCa                                                                                                                                                                  <  2:595629/288‑1 (MQ=255)

                                                                                                                                   cGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTTTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTCCGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATcc                                                                                                                                                               >  1:931928/1‑287 (MQ=255)

                                                                                                                                         aGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAAAGAAG‑GCGCAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTTTTTCCACCCCCGGGACAGTTAACGGCATCCGcctccc                                                                                                                                                        >  2:1507084/1‑283 (MQ=255)

                                                                                                                                           ccTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCcaca                                                                                                                                                      <  2:598589/288‑1 (MQ=255)

                                                                                                                                            cTTTGGTTACCGTGACCGGTTGCTTCGCCATGTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGTCCCGGTGTACTGGTGCGCGGTTCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCcac                                                                                                                                                       <  2:621046/286‑1 (MQ=255)

                                                                                                                                                     ccgtgaccgtTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGt                                                                                                                                                                                                                                                           <  2:1552879/179‑1 (MQ=255)

                                                                                                                                                             gtTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATGGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTc                                                                                                                                   <  1:641144/289‑1 (MQ=255)

                                                                                                                                                                ttcttcGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACCCCGCTTTTTGCAAACAGTCCgt                                                                                                                                >  2:1603379/1‑288 (MQ=255)

                                                                                                                                                                 tcttcGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGt                                                                                                                               <  1:1507084/289‑1 (MQ=255)

                                                                                                                                                                           tttCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTggcg                                                                                                                     <  1:861421/289‑1 (MQ=255)

                                                                                                                                                                           tttCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTggcg                                                                                                                     <  1:594507/289‑1 (MQ=255)

                                                                                                                                                                            ttCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTgg                                                                                                                       <  1:1026472/286‑1 (MQ=255)

                                                                                                                                                                               tGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGt                                                                                                                 <  2:209871/289‑1 (MQ=255)

                                                                                                                                                                                    ggCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGGCTGTTCTTGGCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCCAACAGTCCGGTTTTACTGGCGGCGTCTAAc                                                                                                            >  1:1196772/1‑289 (MQ=255)

                                                                                                                                                                                     gCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGCTCGGCTTTACTCGCGGCGTCTAACa                                                                                                           >  1:957224/1‑289 (MQ=255)

                                                                                                                                                                                       tggtatagagaacaggCCAGAGACTTTTTGCATCCATAGCAACAGACCCAGCGCCGGTGGACAGGTGGGCGGATCCTGATAAATCACCCGCCAGTTTTGTGCGCTCTCCACCAGTTCGTTCAGGCTTTGTTGCGGGTTTGTCAGTTTGTTCTTGTCAAAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGGGCGAAAGTGTCATTATTCAAGCCGCCGGGAACGTTAACGGCATCCGCTGCCTCTCCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACa                                                                                                           <  2:1539259/272‑1 (MQ=255)

                                                                                                                                                                                               cGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCggg                                                                                                                                                                               >  1:457111/1‑211 (MQ=255)

                                                                                                                                                                                               cGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCggg                                                                                                                                                                               <  2:457111/211‑1 (MQ=255)

                                                                                                                                                                                               cGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTggcg                                                                                                                     >  1:525416/1‑269 (MQ=255)

                                                                                                                                                                                               cGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTggcg                                                                                                                     <  2:525416/269‑1 (MQ=255)

                                                                                                                                                                                               cGTCATCGCCATAGACTTTTTGCATACATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGTC‑ATAATCAAACGGTAAGAAAGTGTCATTATTCCAGCCGCCGGGAAAGTTGACGGCATCCGCTGCCACCACGCCTTTGGCAAACAGTCCGGTTTTACTGGCGGGGTCTAACAGgttttttt                                                                                                  >  1:613409/1‑286 (MQ=255)

                                                                                                                                                                                                gTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATcc                                                                                                <  1:337467/289‑1 (MQ=255)

                                                                                                                                                                                                         aTAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGc                                                                                       <  1:1603379/289‑1 (MQ=255)

                                                                                                                                                                                                                tgttGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCCCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACAt                                                                                <  2:778612/287‑1 (MQ=255)

                                                                                                                                                                                                                gttttCATCCATCGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACAt                                                                                <  1:998426/288‑1 (MQ=255)

                                                                                                                                                                                                                 tttGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGc                                                                                                                                                                                                                                                                       >  2:470668/1‑107 (MQ=255)

                                                                                                                                                                                                                 tttGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGc                                                                                                                                                                                                                                                                       <  1:470668/107‑1 (MQ=255)

                                                                                                                                                                                                                 tttGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGGGTCATTTTTCCAGCCGCCGGGAACCTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATccccggctctttttccac                                                                                >  1:1539259/1‑272 (MQ=255)

                                                                                                                                                                                                                       tCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTCTTTCAGTTTTTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTTACGGCCTCCGCCCACACACCGCTTTTTGCAAACAGTCCGGTTTTACTGCCCGCTTCTAACAGGTTGTTCACCAGCCCCacacctccacccgcctt                                                                         >  2:920203/1‑273 (MQ=255)

                                                                                                                                                                                                                                 cAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAg                                                                                                                                                                                                                                                                                          >  2:1520908/1‑72 (MQ=255)

                                                                                                                                                                                                                                 cAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAg                                                                                                                                                                                                                                                                                          <  1:1520908/72‑1 (MQ=255)

                                                                                                                                                                                                                                     caccgccccgtatgacgggtgaccgGACCCTAATGAATCCCCCGCCAGTTTCGATCTCGTGCAACCAGTTCTTTCAGGCGTGAGGGCGGGTTTTTACGTTTCTTCTAGTCAGAAACGAAG‑GCGAAGTAGCC‑ATAGTCAAACCGTTCGAAAGCGTCATTATTCCAGCCGCCGGGAACGTTAACGGCCCCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTGATCCAGCCCCAGCACCACCTCGGCTTTACTGTTTTTGCCtt                                                           <  2:1296614/266‑1 (MQ=255)

                                                                                                                                                                                                                                              ggTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACATCGGCTTTACTGTTTTTGCCTTCCATCCGTa                                                  <  1:1090432/289‑1 (MQ=255)

                                                                                                                                                                                                                                               gtgtACTGGTGCGCGGAGCCTAATACATCACCCGCCATTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCAGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAGCAGGTTGTTATCCAGCCCCAGCACCACATCGGCTTTACTGTTTTTGCCTTCCATCCGTa                                                  <  2:957224/288‑1 (MQ=255)

                                                                                                                                                                                                                                                tgtACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGgtt                                                                                                       <  1:558515/234‑1 (MQ=255)

                                                                                                                                                                                                                                                         gcgcGGATCCTTAGAAATCACCCGCCAGTTTTGATCGCTCGCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTGTTCAGTTTGTTCTGGGCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAACCGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACATCGGCTTTACTGTTTTTGCCTTCCATCCGTAGACGGTTgag                                        <  2:931928/288‑1 (MQ=255)

                                                                                                                                                                                                                                                         gcgcGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACATCGGCTTTa                                                                        >  2:1427306/1‑256 (MQ=255)

                                                                                                                                                                                                                                                         gcgcGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACATCGGCTTTa                                                                        <  1:1427306/256‑1 (MQ=255)

                                                                                                                                                                                                                                                         gcgcGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACATCGGCTTTACTGTTTTTGCCTTCCATCCGTAGACGGTTgaga                                       <  1:353198/289‑1 (MQ=255)

                                                                                                                                                                                                                                                         gcgcGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACATCGGCTTTACTGTTTTTGCCTTCCATCCGTAGACGGTTgaga                                       >  1:1018197/1‑289 (MQ=255)

                                                                                                                                                                                                                                                         gcgcGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCCGCGTCTTACAGGTTGTTATCCAGCCCCAGCACCACATCGGCTTTACTGTTTTTGCCTTCCATCCGTAGACGGTTgaga                                       >  1:821533/1‑289 (MQ=255)

                                                                                                                                                                                                                                                          cgcgGATCCTGATAAATCACCCGCCAGTTTTTATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGGCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGATGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCCGCACCACATCGGCTTTACTGTTTTTGCCTTCCATTCGTAGACGGTTgagca                                      >  1:1146838/1‑287 (MQ=255)

                                                                                                                                                                                                                                                          cgcgGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑CTAATCCAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTATCACAGGTTGTTATCCAGCCCCAGCACCACACTGGTCTTCCTGTTTTTGCCTTCCATCCTTACACGGTTGGGaa                                      >  2:843442/1‑289 (MQ=255)

                                                                                                                                                                                                                                                          cgcgGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTAAGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACATCGGCTTTACTGTTTTTGCCTTCCATCCGTAGACGGTTGAGaa                                      >  2:90011/1‑289 (MQ=255)

                                                                                                                                                                                                                                                            cgGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAAGGAAA‑GCGAAATATCC‑ATAAATAAAAGGTACGAAAGGGTCATTATTACAGACGCCGGGCAGGGTAAAGGCCTCCCCCGCCACGCCGCTTTTCGCAAAAAGCCCGGTTTTACTTGCGGGCACTAACCGGTTGTTTACCAGCACCAACCCAACACCGGCTTTACCTTTTTTGCCTTCACTCCGTAAACGGTCGACAAGc                                    >  2:1610818/1‑289 (MQ=255)

                                                                                                                                                                                                                                                            cgGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACCACGCTTTTGGCAAACAGGCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACATCGGCTTTACTGTTTTTGCCTTCCATCCGTAGACGGTTGAGAAGc                                    >  1:1530860/1‑289 (MQ=255)

                                                                                                                                                                                                                                                            cgGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACATCGGCTTTACTGTTTTTGCCTTCCATCCGTAGCCGGTTGAGAAGc                                    >  1:676739/1‑289 (MQ=255)

                                                                                                                                                                                                                                                                    gATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTGTGTGGCGGGTTTTTAAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACATCGGCTTTACTGTTTTTGCCTTCCATCCGTAGACGGTTGAGAAGCGAAACGcc                            <  2:835309/289‑1 (MQ=255)

                                                                                                                                                                                                                                                                         aTCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCCAACCGCGTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACATCGGCTTTACTG‑TTTTGCCTTCCATCCGTAGACGGTTGAGAAGCGAAACGCCATCtt                       >  1:89981/1‑288 (MQ=255)

                                                                                                                                                                                                                                                                         aTCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTCAGGTTGTTCTTGTCATAAAAGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTAAGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTTGCTGCGGCTAACAGGTTGTTATCCAGCCCCAGCACCACATCGGCATTACTGTTTTTGCCTTTCATCCGTAGACGGTTGAGAAGCGAAACGCCATCt                        >  1:1272009/1‑288 (MQ=255)

                                                                                                                                                                                                                                                                               cGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACAt                                                                                >  1:1160375/1‑226 (MQ=255)

                                                                                                                                                                                                                                                                               cGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACAt                                                                                <  2:1160375/226‑1 (MQ=255)

                                                                                                                                                                                                                                                                                 ccAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACATCGGCTTTACTGTTTTTGCCTTCCATCCGTAGACGGTTGAGAAGCGAAACGCCATCTTCCAGCGcc               <  1:1610818/289‑1 (MQ=255)

                                                                                                                                                                                                                                                                                    gTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCCCACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACATCGGCTTTACTGTTTTTGCCTTCCATCCGTAGACGGTTGAGAAGCGAAACGCCATCTTCCAGCGccac             >  1:1607200/1‑288 (MQ=255)

                                                                                                                                                                                                                                                                                     ttttGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTTGCGGCGTCCAACAGGTTGTTCTCCAGCCCCAGCACCACATCGGCTTTACTGTTTTTGCCTTCCATCCGTAACAGGTTGAGAAGCGAAACCCCATCTTCCCGCGccccc            >  1:209795/1‑288 (MQ=255)

                                                                                                                                                                                                                                                                                              ttctaAACAAGTCCTTTAGTGCTTTTTGGCGGGTTTTTCAGTTTGTTTTTGTAATAAAAGAAG‑GGGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTTGCAAACAGTCCGGTTTTACTGGCTGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACATCGGCTTTACTGTTTTTGCCTTCCATCCGTAGACGGTTGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGtt  <  2:613409/288‑1 (MQ=255)

                                                                                                                                                                                                                                                                                              ctctCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAGCGAAG‑GCGAAGTAGCC‑AAAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACATCGGCTTTACTGTTTTTGCCTTCCATCCGTAGACGGTTGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGtt  <  1:1174020/289‑1 (MQ=255)

                                                                                                                                                                                                                                                                                              ctctCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACATCGGCTTTACTGTTTTTGCCTTCCATCCGTAGACGGTTGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGtt  <  2:1607200/289‑1 (MQ=255)

CAGATAGTGACCTTCGCTGAAGTTCGCGGCGGCGTAGTTATCTTTCTTCTCTTCGAGAATGTGATAAGCCGGAGAGGTGGTGTAACTCAGTACCAGATCGCTTTCACCTTTTAAAAACAGGCCGTAGGCTTCGCTCCAGCCTTTGGTGACCGTGACCGTTTTCTTCGCCAGTTTCTGCCAGGCTTGTGGGGCGTCATCGCCATAGACTTTTTGCATCCATAGCAACAGACCCAGCCCCGGTGTACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAG‑GCGAAGTAGCC‑ATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGAACGTTAACGGCATCCGCTGCCACACCGCTTTTGGCAAACAGTCCGGTTTTACTGGCGGCGTCTAACAGGTTGTTATCCAGCCCCAGCACCACATCGGCTTTACTGTTTTTGCCTTCCATCCGTAGACGGTTGAGAAGCGAAACGCCATCTTCCAGCGCCACCAGTTTCAGTT  >  NC_000913/74755‑75329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`