Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,296,189 A→G 39.3% intergenic (+396/+248) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,1890AG39.3% 23.7 / 48.7 56intergenic (+396/+248)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
Reads supporting (aligned to +/- strand):  ref base A (15/16);  new base G (11/9);  total (31/25)
Fisher's exact test for biased strand distribution p-value = 7.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-01

ATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACC  >  NC_000913/4295902‑4296471
                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                          
aTTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTa                                                                                                                                                                                                                                                                                            >  2:642425/1‑288 (MQ=35)
     gTAGGCCGGATAAGGTGGTTATTGCCCACAGGGAATCAACGCCAGTTCGTAGGCTTAACGCGTCTTAGCAGCCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGt                                                                                                                                                                                                                                                                                      <  2:813240/289‑1 (MQ=11)
     gTACGTCGGAGAAGGCGATTCTGCCGAATCCGCCATCAACGCCTTATGCGACGTTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGTCGTTTACGCCGCATCCGACATCAACGCCTGATGCAACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGt                                                                                                                                                                                                                                                                                      <  1:1346267/289‑1 (MQ=11)
           cGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTCACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTACCGTGACTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCCCAGACACCGCACTAGCCTGCTTTACCGTGATCTCGTAGCTCGGATCAGGCGTTTACGCCTCATCCGACACCCACTCCTGATTCGTCGCCTCAcgctaattgt                                                                                                                                                                                                                                                                                 >  2:903775/1‑281 (MQ=11)
           cGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATATCCTGATTTCGCGTGACTTTGTAGGTCGGATACGGCGTTTATGCCGCATCCGACACCCACGCCTGATTCGACCCTTTACGCGTCTTATc                                                                                                                                                                                                                                                                                >  2:316616/1‑289 (MQ=255)
           cGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTAt                                                                                                                                                                                                                                                                                 <  1:1072154/288‑1 (MQ=37)
           cGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATc                                                                                                                                                                                                                                                                                >  2:490043/1‑289 (MQ=37)
               tAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGc                                                                                                                                                                                                                                                                            <  1:642425/289‑1 (MQ=37)
               tAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGc                                                                                                                                                                                                                                                                            <  1:1066437/289‑1 (MQ=37)
                aaGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAgg                                                                                                                                                                                                                                                                             >  1:1472615/1‑287 (MQ=37)
                        tATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATATCCTGATTTAGCGTGATTTTGTAGGGCGGCTAAGGCGTTTATGCCGCATCCGACCTCAACGCCTGATGAGACGCTTACAGCGTCTTATCCGGCCTACTCCaa                                                                                                                                                                                                                                                                   >  2:180310/1‑288 (MQ=255)
                         cggCCGCATCCGACATCAACGCCTGCTGCGACACTTACCGCGTGTTATCAGGCCGACGCCAGAGAGCGGAATAGCCTGATTTAGCTTGATTTAGTAGGTATGATAAGGCGTTTACGCCCCACCCAACATCAACGCCTGATGCGACGCGTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGa                                                                                                                                                                                                                                                                  <  2:764189/287‑1 (MQ=11)
                         attccgaaaccGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGa                                                                                                                                                                                                                                                                  <  1:1210073/280‑1 (MQ=25)
                         agcggccatcggacagatgcGCCGGATGGGACGCTTAACGCGTCTTAACCGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGa                                                                                                                                                                                                                                                                  <  2:1473471/270‑1 (MQ=11)
                               cATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCTAGACCGCGCAATAGCCTGATTTAGCGTCATTTGGTAGGTCGGATAAGGCGTTTATGCCGCATCTGACATCAAAGCCTGATGGTACGCTTGACGCGTCGTATCAAGACTaacaccacgaggca                                                                                                                                                                                                                                                            >  2:527243/1‑276 (MQ=11)
                               cATCCGACATCAACGCCTGATGCCACGATCGCGGCGTCTTATCCGGCCTACGCCAGACAGCGCAATAGCCTCAGTAAGCGCTATTTTGTCGGTCGGATCAGGCGCTTACGCCGCATCCGACATCAACGCCTGATGCGACGTTTCACGCGCCTTATCAGGCCTACCCCAGCCAGCGCAATAACCTGATTTAGCGTGACTTTGTCGGTCGGATAAGGCGTTAACGCCGACTCCGACATCAATGCCTGATTCGACGTTTTCCGCGTCTTATCAGGCCTatcttaaccgttgg                                                                                                                                                                                                                                                            >  2:234075/1‑276 (MQ=255)
                                 tCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACTCCAGACATCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATACGTCGTTTATGCCGCATCCGACATAAACGCCTGCTTCGACGTTTAGCGCGTCTTTACATGCCTACGcacgacctcggaa                                                                                                                                                                                                                                                          >  2:1155174/1‑277 (MQ=11)
                                 tCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGCCAGCGCAATAGCCTGATCTCGCGTGATTTTGTAGGTCGGATACTGCGTTTATGCCCCATCCCACCTCAACGCCCGATTAGACCTTTTCCGTGTCTTTACAGGACTACGCCAACCAGCCCa                                                                                                                                                                                                                                                           >  2:1194437/1‑288 (MQ=11)
                                            cGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCCGAttt                                                                                                                                                                                                                                               >  1:252912/1‑289 (MQ=25)
                                               cTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGACTTTGGAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCCGATTCGACGCTTGCCGCCTCTTATCAGGCCTACGCCAGCCCGCGCACACGCCTGATTAAGc                                                                                                                                                                                                                                            >  2:759789/1‑289 (MQ=11)
                                                       aCGCTTAACGCGTCTTATAAGGCCTACGCCAGACAGCGCACTAACCTGATGTAGAGTGAATTTTAAGGTGGGATAAGCCGTTTACGCCCCATCCGCCATCAAGGACTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAATCCAGACAGCGCAATAGCGTGACTTAGAGTCATTGTGTAGGTCGGATAAGGCGTTTATGCCGCCTCCGAACTCATACCTTGACGAGTCGCTTACCGCGTCTTAGCATGCCTCTGCAAAACAGCGCAACACCCttgttgacggtgagctg                                                                                                                                                                                                                                    >  2:67267/1‑273 (MQ=12)
                                                             aaCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCTTCTTATCAGGCCTACCCCAGCCAGCTCAATAGCCTGATTCAGCGTGATTTTGTAgg                                                                                                                                                                                                                               >  2:280164/1‑288 (MQ=16)
                                                                   gcTTATCAGGCCGACGCCAGACAGGCCATTAGCCTAATTTAGCGTGATTTTGTGGGGCGGATAAGGCGTTTCCGCCGCTTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGAt                                                                                                                                                                                                                         <  2:423686/287‑1 (MQ=255)
                                                                    cTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCTCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTTATTTTGTAGGTCGGATaa                                                                                                                                                                                                                       >  1:1366371/1‑289 (MQ=18)
                                                                            gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGttt                                                                                                                                                                                                                >  1:1143523/1‑288 (MQ=33)
                                                                            gCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTGATGCCGCATCCGACATCAACGACTGATGCGACGCTGCACGCGTCTTATCAGGCCTAAGCCACGCAGCGCAACAGCCTGACTGTGCATTCTTTTGTATGTCGTACAAGGCCTTTa                                                                                                                                                                                                               >  2:1319800/1‑289 (MQ=11)
                                                                                 cGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTAt                                                                                                                                                                                                                                                                                 <  2:110764/218‑1 (MQ=34)
                                                                                    caaaCAGCGGAATAGCCGGGTTTAGCGTGATTTTGTGGGTCCGCTAAGCCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCa                                                                                                                                                                                                        <  2:1198077/285‑1 (MQ=255)
                                                                                           cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACg                                                                                                                                                                                                                                                                       >  1:110764/1‑218 (MQ=34)
                                                                                           cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTCAAGCCGCATCCGACa                                                                                                                                                                                                 >  1:523998/1‑288 (MQ=18)
                                                                                                        tttAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCACTAGCCTTCTTTCGCGTTATTCTGTATATCCGATACGGCGTTTAAGCCACCTCCGACATAAATGCATGAtt                                                                                                                                                                                    >  2:940971/1‑287 (MQ=11)
                                                                                                                gATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGc                                                                                                                                                                                                                                                                            <  1:1446381/192‑1 (MQ=34)
                                                                                                                          gTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGa                                                                                                                                                                                                                                                                  >  2:1446381/1‑192 (MQ=34)
                                                                                                                                                 aTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGc                                                                                                                                                                                                                                                      <  1:191870/181‑1 (MQ=33)
                                                                                                                                                 aTCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGc                                                                                                                                                                                                                                                      >  2:191870/1‑181 (MQ=33)
                                                                                                                                                           aaCGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGc                                                                                                                                                                                                                                                                            <  1:605414/149‑1 (MQ=32)
                                                                                                                                                                                      ttagtagccttactgccgttggcactttAGCCTTGTTTAGTGGGAGTTTTTTGGTCGTGTGAGTCGTTTCTGCCCCGTCCTGCGTCAACGCCTGGTTCGACGCTTACCGCGTCTTATCGGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAg                                                                                                     <  2:175201/262‑1 (MQ=11)
                                                                                                                                                                                                                                   gtttgTAGGTCTGGAATGGCGGTTGTGGCGAGTCGGGGATGAACTCGTTATGCGACGCTTAACACGTCTTAGCAGGCCTACGCCAGAGAGCGCAATAGCCTTATTTAGCGTGATTTTGTAGGGCGGATAAGGCGTTTACGCCCCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCaacgcctgatgcgacgcttaa                                                         >  2:1216365‑M2/2‑267 (MQ=255)
                                                                                                                                                                                                                                             cGGATAAGGCGTTTACGCCGCATCCGGCATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCaatgcctgatgcgacgcttgccgcgtcttat                                               >  1:860631‑M2/1‑257 (MQ=255)
                                                                                                                                                                                                                                             cGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACCGCGCCATAGCCTGATTTACCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCcatgcctgatgccacccttgccgcgtcttatc                                              <  2:537021‑M2/289‑33 (MQ=255)
                                                                                                                                                                                                                                             cGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCATTAGCCTGATTTAGCGTGCTTTTGTAGTTCGGATACGCCGTTTACGCCGCCTCCGACATCcatgcctgatgcgacgctggcctcgtcttatc                                              <  2:1414329‑M2/289‑33 (MQ=255)
                                                                                                                                                                                                                                             cGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCaatgcctgatgcgacgcttgccgcgtcttatc                                              <  1:268745‑M2/289‑33 (MQ=255)
                                                                                                                                                                                                                                             cGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCaatgcctgatgcgacgcttgccgcgtcttat                                               >  1:490043‑M2/1‑257 (MQ=255)
                                                                                                                                                                                                                                             cGGATAAGGCGTTTACGCCGCATCCGACATAAAAGCCTGATGGGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGAAAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCaatgcctgatgcgacgcttgccgcgtcttat                                               >  1:317463‑M2/1‑257 (MQ=255)
                                                                                                                                                                                                                                                 gaggcgatttacggcgcatacgagaaacaCGCCGGATGGGACGCTTGCCGAGTCTTATCCGGCCTACGCCAGACAGCGAAATAGCCTGATTTAGCGTGATTTTGTGGGTCGGATAAGGGGTTGATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGAGTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAgg                                           <  2:983126/260‑1 (MQ=11)
                                                                                                                                                                                                                                                 caagggagttacgccgctTACGGAATCAAAGCCTGATGGTACGCTTGCCGCGGCTTATCAGGCCTACGTCAGACAGCGCAATAGCCTGATTGAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCaatgcctgatgcgacgcttgccgcgtcttatcaggc                                          >  2:197323‑M2/9‑253 (MQ=255)
                                                                                                                                                                                                                                                             gCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCg                              <  1:180310/289‑1 (MQ=25)
                                                                                                                                                                                                                                                                              cgctggATGCGACGCTTGCCGCGTCTGATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTGAGCGTGATTTTGTAGGTCGGATAAGGCGTTTAGGCCTCATCCAACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCCGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaaa             <  1:619577/284‑1 (MQ=14)
                                                                                                                                                                                                                                                                              cGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCTTACGCCAGACCGCGCAATAGCCTGATTTCGCGTGATTTTGTAGGTCGGCTAAGGCGATTACGCCCCATCCGACCTCAATGCCTGACGCGACGCTCGCCGTGTCCTATCAGCGCGATCTTCACCGTTGCTGCAttttttca              >  2:129845/1‑285 (MQ=11)
                                                                                                                                                                                                                                                                              cGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAAAGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGCTTTAGCGTGATTTTGTAGGTCGTACAAGGCGTTTACGCCGCATCCGACATCAATGCCTGTTGCGACGCTTGCCGCGTCTTATTCGGCCTAACTTCACCCTTGGTTAATTTTTcca              >  2:65217/1‑286 (MQ=11)
                                                                                                                                                                                                                                                                               gCCTGAGGCGACTCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTATGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAAc            <  1:527243/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACCTCAATGCCTGATTCGACGCTTGCCGCGTCTTAACAGGCCTATCTTAACCTCTGGTTAATTTTTCAAAc            >  2:1153277/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                    aTGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTAAAACTCtt        >  2:467175/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                     tggggcGCTTGCCGAGTCTTATCAGGCCTACGCAAGACAGCGCAATAGCCTGATGTAGCGTGATTTTGGAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCGACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTca      <  1:903775/284‑1 (MQ=255)
                                                                                                                                                                                                                                                                                         aCGCTTGCCGCATCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACCTTAACCGTTGGTTAATTTTTCAAACTCTTCacac   >  1:535821/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                          cGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACAcc  <  1:280164/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                                          cGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCg                                                                                                                                                                                  <  1:433657/112‑1 (MQ=32)
                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                          
ATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTACGCCGCATCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAAACTCTTCACACC  >  NC_000913/4295902‑4296471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: