Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 1,571,844 | C→T | 4.1% | D68N (GAC→AAC) | gadB ← | glutamate decarboxylase B, PLP‑dependent |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 1,571,844 | 0 | C | T | 4.1% | 345.3 / 2.8 | 111 | D68N (GAC→AAC) | gadB | glutamate decarboxylase B, PLP‑dependent |
Reads supporting (aligned to +/- strand): ref base C (45/61); new base T (3/2); total (48/63) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.50e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 115 total aligned reads displayed.
CCGGACCGCACACCAGGTTTGGTTTATCCGTTGGTTTGCCTGCAGCTTCCATACGCTTGCGCCAACGCCATTTCATCGCCATCCCGCCGAGCATACAGGCCTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑ATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTATCCATTTTAAACTCCTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTCGTGT > NC_000913/1571562‑1572125 | ccGGACCGCACACCAGGTTTGGTTTATCCGTTGGTTTTCCTGCAGCTTCCATCCGCTTGCGCCAACGCCATTTCATCGCCATCCCGCCGAGCATACAGGCCTCGGAAGAACCAATGGTGTTGGTGCCAACGTCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGt < 2:686791/289‑1 (MQ=37) ccGGACCGCACACCAGGTTTGGTTTATCCGTTGGTTTGCCTGCAGCTTCCATACGCTTGCGCCAACGCCATTTCATCGCCATCCCGCCGAGCATACAGGCCTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGt < 1:505146/289‑1 (MQ=37) ccGGACCGCACACCAGGTTTGGTTGATCCGTTGCTTTGCCTGCAGCTTCCATACGCTTGCGCCAACGCCATTTCATCGCCATCCCGCCGAGCATACAGGCCTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGt < 2:990401/289‑1 (MQ=37) gACCGCACACCAGGTTTGGTTTATCCGTTGGTTTGCCTGCAGCTTCCATACGCTTGCGCCAACGCCATTTCATCGCCATCCCGCCGAGCATACAGGCCTCGGAAGAACCAATGGTGTTGGTGCCCACGGCCTGACCATTTTTCGTCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCCATTTGTGGACA‑TTTTAGTCATCACAGTTCTg > 1:671282/1‑289 (MQ=255) gACCGCACACCAGGTTTGGTTTATCCGTTGGTTTGCCTGCAGCTTCCATACGCTTGCGCCAACGCCATTTCATCGCCATCCCGCCGAGCATACAGGCCTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTg > 1:714386/1‑289 (MQ=37) gACCGCACACCAGGTTTGGTTTATCCGTTGGTTTGCCTGCAGCTTCCATACGCTTGCGCCAACGCCATTTCATCGCCATCCCGCCGAGCATACAGGCCTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTg > 1:469758/1‑289 (MQ=37) ccGCACACCAGGTTTGGTTTATCCGTTGGTTTGCCTGCAGCTTCCATACGCTTGCGCCAACGCCATTTCATCGCCATCCCGCCGAGCATACAGGCCTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGc < 1:739141/289‑1 (MQ=37) cacaCCAGGTTTGGTTTATCCGTTGGTTTGCCTGCAGCTTCCATACGCTTGCGCCAACGCCATTTCATCGCCATCCCGCCGAGCATACAGGCCTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTAACCAACGCAGGTCGCTGGCTGCGGAATGCGGATATTCTTATTTGTCGATCCAGCTTTTGTTAATGGATAAATCCATCAATTTGTTGACATTTTTCGT‑GTTCCAGGTCTGGCAGa > 1:998844/1‑289 (MQ=11) cacaCCAGGTTTGGTTTATCCGTTGGTTTGCCTGCAGCTTCCATACGCTTGCGCCAACGCCATTTCATCGCCATCCCGCCGAGCATACAGGCCTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTGTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCCACGCACGCCGAGGGCTGCGGATTGCGGAGATTCTTCTTTGTCGCTCCCGTTTTTGTTAATGGGTAAATCCATCAATTTGTGGCCA‑TTTTCGTCGTCCCAGGTCTGGCAGa > 2:429080/1‑289 (MQ=11) acacCAGGTTTGGTTTATCCGTTGGTTTGCCTGCAGCTTCCATACGCTTGCGCCAACGCCATTTCATCGCCATCCCGCCGAGCATACAGGCCTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGaa < 1:981568/289‑1 (MQ=37) cacCAGGTTTGGTTTATCCGTTGGTTTGCCTGCAGCTTCCATACGCTTGCGCCAACGCCATTTCATCGCCATCCCGCCGAGCATACAGGCCTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGaaa < 2:714386/289‑1 (MQ=37) gtttggtttATCCGTTGGTTTGCCTGCAGCTTCCATACGCTTGCGCCAACGCCATTTCATCGCCATCCCGCCGAGCATACAGGCCTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGcc > 1:68315/1‑289 (MQ=37) tttggtttATCCGTTGGTTTGCCTGCAGCTTCCATACGCTTGCGCCAACGCCATTTCATCGCCATCCCGCCGAGCATACAGGCCTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGcc < 2:700719/288‑1 (MQ=37) tggtttATCCGTTGGTTTGCCTGCAGCTTCCATACGCTTGCGCCAACGCCATTTCATCGCCATCCCGCCGAGCATACAGGCCTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAgg > 2:876479/1‑289 (MQ=37) tggtttATCCGTT‑GTTTGCCTGCAGCTTCCATACGCTTGCGCCAACGCCATTTCATCGCCATCCCGCCGAGCATACAGGCCTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGt > 2:559797/1‑289 (MQ=37) tCCGTTGGTTTGCCTGCAGCTTCCATACGCTTGCGCCAACGCCATTTCATCGCCATCCCGCCGAGCATACAGGCCTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGAc < 2:1000749/289‑1 (MQ=37) tGGTTTGCCTGCAGCTTCCATACGCTTGCGCCAACGCCATTTCATCGCCATCCCGCCGAGCATACAGGCCTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGcc < 1:637824/274‑1 (MQ=37) tGGTTTGCCTGCAGCTTCCATACGCTTGCGCCAACGCCATTTCATCGCCATCCCGCCGAGCATACAGGCCTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGcc > 2:637824/1‑274 (MQ=37) tGGTTTGCCTGCAGCTTCCATACGCTTGCGCCAACGCCATTTCATCGCCATCCCGCCGAGCATACAGGCCTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCg < 2:68315/289‑1 (MQ=37) tGGTTTGCCTGCAGCTTCCATACGCTTGCGCCAACGCCATTTCATCGCCATCCCGCCGAGCATACAGGCCCCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACC‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGCGGCCAGGTTCTGAAGAGCg > 1:478375/1‑289 (MQ=25) tGCCTGCAGCTTCCATACGCTTGCGCCAACGCCATTTCATCGCCATCCCGCCGAGCATACAGGCCTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTGGTCGCCCTAGGTCTGGCATAAAGTGGCCAGGTTCGTACGAGCCttcc > 1:113535/1‑286 (MQ=255) tGCCTGCAGCTTCCATACGCTTGCGCCAACGCCATTTCATCGCCATCCCGCCGAGCATACAGGCCTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCCTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAATGGGCCAGGTTCTGACGAGCGttcc > 1:962571/1‑286 (MQ=255) gCCTGCAGCTTCCATACGCTTGCGCCAACGCCATTTCATCGCCATCCCGCCGAGCATACAGGCCTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCa < 2:536355/289‑1 (MQ=37) ccTGCAGCTTCCATTCGCTTGCGCCAACGCCAGTTCTTCGCCATCCCGCCGAGCATACAGGCCTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCCTGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGAGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCAt < 2:169308/289‑1 (MQ=255) ccTGCAGCTTCCATACGCTTGCGCCAACGCCATTTCATCGCCATCCCGCCGAGCATACAGGCCTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCa > 1:877306/1‑288 (MQ=37) cTGCAGCTTCCATACGCTTGCGCCAACGCCATTTCATCGCCATCCCGCCGAGCATACAGGCCTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCAt < 2:573971/288‑1 (MQ=37) tGCGCCAACGCCATTTCATCGCCATCCCGCCGAGCATACAGGCCTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGcc > 1:244311/1‑248 (MQ=38) tGCGCCAACGCCATTTCATCGCCATCCCGCCGAGCATACAGGCCTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGcc < 2:244311/248‑1 (MQ=38) gcgcCAACGCCATTTCATCGCCATCCCGCCGAGCATACAGGCCTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTg > 1:827076/1‑289 (MQ=38) cgcCAACGCCATCTCATCGCCATCCCGCCGAGCATACAGGCCTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGATCGTTGCCATCCAGATA‑TAATTCGGCATTGa > 1:622034/1‑289 (MQ=255) aCGCCATCTCATCGCCATCCCGCCGAGCATACAGGCCTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATc < 2:622034/289‑1 (MQ=38) gCCATTTCATCGCCATCCCGCCGAGCATACAGGCCTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTg < 2:469758/289‑1 (MQ=38) aTTTCATCGCCATCCCGCCGAGCATACAGGCCTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGTCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGaa < 2:947142/289‑1 (MQ=39) aTTTCATCGCCATCCCGCCGAGCATACAGGCCTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGaa < 1:431213/289‑1 (MQ=39) tCGCCATCCCGCCGAGCATACAGGCCTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCAt > 2:315327/1‑252 (MQ=38) tCGCCATCCCGCCGAGCATACAGGCCTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCAt < 1:315327/252‑1 (MQ=38) cGCCATCCCGCCGAGCATACAGGCCTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCCAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACa > 1:611392/1‑289 (MQ=39) aGCATACAGGCCTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCAt < 1:801514/256‑1 (MQ=39) aGCATACAGGCCTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCAt > 2:801514/1‑256 (MQ=39) aCAGGCCTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGc > 1:416630/1‑289 (MQ=40) ccTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCa < 1:107329/285‑1 (MQ=40) cTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATCAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TACTTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGa > 2:885121/1‑289 (MQ=255) tCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGaa > 2:192220/1‑289 (MQ=40) tCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGaa > 1:714701/1‑289 (MQ=40) tCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGaa > 1:800001/1‑289 (MQ=40) tCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGaa > 2:768369/1‑289 (MQ=40) ggAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGaaaa < 2:800001/289‑1 (MQ=40) aaCCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTTCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGttt > 2:1037724/1‑289 (MQ=255) aaCCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGttt > 2:663273/1‑289 (MQ=255) aTGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGAAAAGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCAAGTTTTTGTTAATGGATAAATCCCTCCATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAACTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGGCCCTTTCGTGCAG‑CGGACAACGTTTTGAtt > 2:492741/1‑289 (MQ=14) ggTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTTGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATCCGTCATTGATAATCTGGAATGCGAAATCGTCGCACATTTCGTTCAG‑CGGAAAACGTTTTGATTCt > 2:474904/1‑289 (MQ=255) tGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑At < 2:877306/289‑1 (MQ=255) tGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑ATAg < 1:559797/288‑1 (MQ=255) tGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑ATAg < 2:714701/288‑1 (MQ=255) tGCCAACGGCCTGACCAGTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑ATAg < 1:1042397/288‑1 (MQ=255) cGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑ATAGTGGAAAt > 1:1034349/1‑289 (MQ=255) ttttCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTTCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑ATAGTGGAAATAGACTTCGCAcc > 1:369393/1‑289 (MQ=255) ttttCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑ATAGTGGAAATAGACTTCGCAcc > 2:123984/1‑289 (MQ=255) ggcgccggcgcaTTCCACAGATCGGCAACCATATTTACGCAACGCAGGGCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCAATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑ATAGTGGAAATAGACTTCGCACCAAAAc < 2:113535/289‑1 (MQ=255) ggcgcaggcgcaTGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑At < 1:178086/263‑1 (MQ=255) ggcgcaggcgcaTGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑At > 2:178086/1‑263 (MQ=255) cgcaTGCCACAGATCGGCAACCCTATTTACGCATCGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTGGTCGTTCCAGTTTTTGTTAAAGGATAAATCCAGCAATTTGTGGACA‑TTTTCGTCGACCCATGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑ATAGTGGAAATAGACTTCGCACCAAAACGTGaa < 1:492741/286‑1 (MQ=255) cgcaTGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑ATAGTGGAAATAGACTTCGCACCAAAACGTGaa < 2:614961/286‑1 (MQ=255) aTGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑ATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGag < 1:876479/288‑1 (MQ=255) aGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGAAATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCATCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑ATAGTGGAAATAGAATTCGCACCAAAACGTGAATCGAGTAGTTCCg > 1:680611/1‑289 (MQ=255) tCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCTGATTTCGTGTATGCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTAATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑ATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTcc < 1:980635/285‑1 (MQ=255) ggCAACCATATTTCCGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑ATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCtt < 2:478375/289‑1 (MQ=255) ggCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑ATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCtt > 1:654179/1‑289 (MQ=255) ggCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGAGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTATGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTATGCG‑ATAGTGGAAATAGACTTAGCACCAAAACGTGAATCGAGTAGTTCCGACCtt > 2:391375/1‑289 (MQ=255) cAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCATCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑ATAGTGGAAATAGACTTCGCACCAAAACGTGAATAGAGAAGTTCCGACCTTaa > 2:980635/1‑289 (MQ=255) atatTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATCTTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTCCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑ATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCg > 1:89395/1‑289 (MQ=255) atatTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTTCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑ATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCg < 2:369393/289‑1 (MQ=255) atatTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGAGCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑ATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCg < 2:991519/289‑1 (MQ=255) tACGCAACGCAGGTCGATGGCGTCGGATTGCTGATATTCTTCTTTGTCGTTCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑ATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACt < 1:391375/289‑1 (MQ=255) gcaacgcaGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑ATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACtt > 1:1090948/1‑287 (MQ=255) gcaacgcaGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑ATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGc < 2:654179/289‑1 (MQ=255) cgcaGGTCGATGGCTGCGGATTGCGGATATTATTCTTTGTCGATCCAGGTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGTCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAACATGTTTTGTTTCTGCG‑ATGGTGGAAATAGAATTCGCAACAAAACGTGAATCGAGTAGTTTAGGCTTTAAATACGTTACTTGcttct > 1:475853/1‑289 (MQ=17) tCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑ATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTATc < 2:827076/287‑1 (MQ=255) tGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAAAGTTTTGATTCTGCG‑ATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTATCCAtttt > 1:524525/1‑289 (MQ=255) cTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑ATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTATCCATTTTaa < 2:416630/288‑1 (MQ=255) tgcggattgcggatATTCTTCTTTGTCGATCAAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCATCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑ATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTATCCATTTTaaa < 2:699224/288‑1 (MQ=255) ggcggattgcggatATTCTTCTTTGTCGTTCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑ATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTATCCATTTTaaa < 1:130243/287‑1 (MQ=255) tgcggatATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑ATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTATCCATTTTAAACTCCTTaa < 2:962571/289‑1 (MQ=255) cttcttTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑ATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTATCCATTTTAAACTCCTTAAAATGATTGGa < 2:524525/289‑1 (MQ=255) ttcttTGTCGATCCAGTTTTTGTTTATGGAGAAATCCATCAATTGGTGGACA‑TTTTCGTCGTCCAAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAATATA‑TAATTCGTCCTTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑ATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATTCGTTACTTGCTTCTTATCCATTTTAAACTCCTTAAAATGATTGGAt < 1:474904/289‑1 (MQ=255) cttTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑ATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTATCCATTTTAAACTCCTTAAAATGATTGGATc > 1:407336/1‑288 (MQ=255) cggtCCAGTTTTGGTTAAGAGGGAAATCCATCAATTGGTGGACC‑GTTTCGTCGTCCCATGTCTGGCAGAAAGTGGCCAGCTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGGATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGTG‑ATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGAACTTAAATCCGTTACGTGCTTCTTATCCATTTTAAACTCCTTAAAATGATTGGATCGCATTaa < 1:429080/286‑1 (MQ=255) aTCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCAtt > 1:280787/1‑147 (MQ=255) aTCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCAtt < 2:280787/147‑1 (MQ=255) cAGGTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑GTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑ATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAAGCCGTTACTTGCTTCTTATCCATTTTAAACTCCTTAAAATGATTGGATCGCATTAAAAAAg < 1:885121/289‑1 (MQ=255) gTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑ATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTATCCATTTTAAACTCCTTAAAATGATTGGATCGCATTAAAAAAGTAGGAttt > 1:259119/1‑289 (MQ=255) aTGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑ATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTATCCATTTTAAACTCCTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCg < 2:705622/289‑1 (MQ=255) tGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑ATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTATCCATTTTAAACTCCTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGa < 1:325055/289‑1 (MQ=255) aTTTGTGGACA‑TTTTCGTCGTTCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑ATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTATCCATTTTAAACTCCTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTg < 1:1037724/289‑1 (MQ=255) caa‑TTTTCGTGGTCCAGGGCTTGGAGGAAGGTGGCCAGG‑TCTTGCGAGGGGTTGCATCAAAGTATTAATTGGTCATTGTAAATTTGGAATGGGACACCGTGGCGATTTTCG‑GCGGCCGGAAACCGTTTGGATT‑TGCGAATATGGGAATTAAATTTCGAACCAAACGGTGAAT‑GGGTTATTCCGACCCTTAAACCGTTTCCTGGTTTTTATCCATTTTAAACTCCTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACt < 1:661061/287‑1 (MQ=255) cA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑ATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTATCCATTTTAAACTCCTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACt < 1:192220/289‑1 (MQ=255) tttCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑ATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTAATCAATTTTAAACTCTTTAAAAGGATGGGTTCGAATTAAAAAATAAGAATTTTTCGTTAAGTTAAGAAATTGCAAAGAACTCgt > 2:661061/1‑289 (MQ=18) tcCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTg > 1:602091/1‑74 (MQ=34) tcCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑ATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTATCCATTTTAAACTCCTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAg < 2:961853/259‑1 (MQ=255) tcCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑ATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTATCCATTTTAAACTCCTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAg > 1:961853/1‑259 (MQ=255) | CCGGACCGCACACCAGGTTTGGTTTATCCGTTGGTTTGCCTGCAGCTTCCATACGCTTGCGCCAACGCCATTTCATCGCCATCCCGCCGAGCATACAGGCCTCGGAAGAACCAATGGTGTTGGTGCCAACGGCCTGACCATTTTTCGGCGCAGGCGCATGCCACAGATCGGCAACCATATTTACGCAACGCAGGTCGATGGCTGCGGATTGCGGATATTCTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACA‑TTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATA‑TAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAG‑CGGAAAACGTTTTGATTCTGCG‑ATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTATCCATTTTAAACTCCTTAAAATGATTGGATCGCATTAAAAAAGTAGGATTTATCGATAAAGTAAGCAAGTGCAAAGGACTCGTGT > NC_000913/1571562‑1572125 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |