Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,304,535 A→G 8.7% intergenic (+144/+571) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,304,5350AG 8.7% 140.5 / 3.8 61intergenic (+144/+571)eco/mqoecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base A (28/26);  new base G (3/3);  total (32/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.39e-01

ATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGC  >  NC_000913/2304270‑2304809
                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                  
atgcgtgAAGGCTGCGTTACAAAGGGGAGCGGCCGTTCGGGGTGTATATGCCAGACAATTGAGCTGGGAAGAACCGCGTCTGGAAGGCGGAATGGAAAAGTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGGCAAGTCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATcc                                                                                                                                                                                                                                                             <  1:549248/283‑1 (MQ=18)
           ctgcGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGCAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAAc                                                                                                                                                                                                                                                   >  1:326154/1‑288 (MQ=255)
                 tACAACAGCAAGCTGCCGATCGTGGTTTATACGCCAGACAATGTAAATGTGAAGTACCGCGTGTGGAAGGCGGAAAAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAATTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGGGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGa                                                                                                                                                                                                                                             <  2:960176/288‑1 (MQ=255)
                        gCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGtt                                                                                                                                                                                                                                      >  2:699463/1‑288 (MQ=255)
                          aaGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGc                                                                                                                                                                                                                                    >  1:167167/1‑288 (MQ=255)
                           aGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTg                                                                                                                                                                                                                                     >  2:211588/1‑286 (MQ=255)
                           aGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGATGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCAGCAGCCGACATCCAACGCCCGAGCCGGTTg                                                                                                                                                                                                                                     >  2:1030933/1‑286 (MQ=255)
                                ccGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGCAGTGCGACCCACCGTAGGTCCGACACGTCGGTTCCGCCGCCCCCGCCATCCAACGCCCACGCCGGTCGCctgctga                                                                                                                                                                                                                             >  1:743539/1‑288 (MQ=21)
                                 cGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGACGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGCAGGTCAGATAAGGCGGTCACGCCCCCTCCGCCCTCAAACCCCCGCGCCGGTTGCCTGCTGCg                                                                                                                                                                                                                            >  1:673603/1‑289 (MQ=21)
                                       tggtgTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCAGTCACGCCGCATCCGACATCCAACGCCCGCACCGGTTCCCTCATACGACGCTg                                                                                                                                                                                                                      >  2:338613/1‑289 (MQ=255)
                                        ggtgTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTgg                                                                                                                                                                                                                     <  2:67541/289‑1 (MQ=255)
                                         gtgtagagcgcAGGCAATGTGGATGGGCAGTACCGAGGATGGAAGGCGGCAGGGACACGTGACAACGCGGTAGTGCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACAGCAAACCCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTgg                                                                                                                                                                                                                     <  1:840649/278‑1 (MQ=17)
                                         gtgtATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTgg                                                                                                                                                                                                                     <  2:167167/288‑1 (MQ=255)
                                             ataCGCCAGACAAAGGAGATGGCAAGTGCCGGGACTGGAAGGCGGAAGAGACAAGTGACAACGCGGTAGTGCGCGAAGCTGCCGTGAAGTGCGGCACCCGCTAGGCCAGGCAAGGCGGTCACGCCGCATCCGAGAGCCAACGCCCGAGCGGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGt                                                                                                                                                                                                                <  1:814376/289‑1 (MQ=18)
                                              taCGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGcgcg                                                                                                                                                                                                                 >  2:98973/1‑287 (MQ=255)
                                               cggCCAGACAAAGTGGGTGTGAAGGACCGCGGCGGGCAGTCGGAGGAGAAAATGGACAACGGGGGAGGGCGCGAAGCTGCCGGAAGGTGCGGCACCCCGGAGGTCGGGAAAGGCGGGCACGCGGCAACCGACATCCAGCGCCCGAGCCGGGGGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCTCCCCGTAGGTCAGACAAGGCGGACACGCGGCAGCCGACATCCAACGCCCGGGCCGGTTGCCTGATGGGACGCTGGCGCGt                                                                                                                                                                                                                <  1:973746/285‑1 (MQ=17)
                                                   cgagaaagggagggggtaaGTACCAAGTATGGAAGGCAAAAGAGAGAATGGGCCACTGGGTGGTTCGGGAAACTGCCGGTAAGTGCGGCAGCTCGTGGTGCAGACAAGGCGGTCAGGCCGCATCCGACAGCCAATGCGCGAGCCGGTTGCCGGATGCGACGCTGGCGCGTCTTATCTGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                                                                                                                                           <  1:383331/271‑1 (MQ=14)
                                                   cAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                                                                                                                                           <  2:124442/288‑1 (MQ=255)
                                                    aGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCCGTTGCCTGCTGCGACGCTGGCGCGTCGTCTCa                                                                                                                                                                                                         >  2:938606/1‑289 (MQ=255)
                                                      aCAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTTCGCCACCCCGTAGGTCGGATAAGGCGGTCACGCCGCATCCGACATCCACCGCCCGAGCCGGTTGCCTGATGCGACGCCGGcccggtgtctcag                                                                                                                                                                                                        >  1:202183/1‑279 (MQ=18)
                                                      aCAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGCCAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTCCCTGATGCGACGCTGGCGCGTCTTATCAg                                                                                                                                                                                                        >  2:798943/1‑288 (MQ=255)
                                                             ggaggcgAAGTACCGCGCCAGGAAGGCGAAAGAGAAAATTGACACGGCGGGAGTTCGCGAAAGTGCCGTGAAGGCCGGAACCCGCTGGGTGAGAGAAGGCGGTCACGCAGCATCCGACAGCCAACCCCCGGGCCGGTTGCCGGATGCGACGCTGGCGCGTCTGATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTaca                                                                                                                                                                                                 <  1:430178/282‑1 (MQ=17)
                                                             agagGTGAAGTACCGCGGCTGGAAGGGGGAAGAGCAAAGTGACCGCGCGGTAGTTGGCTAAGCTGCCGTGAAGTGCGGCACCCCGTAGGTCGGACAAGGCGGTCACGCCGCATCCGACATCCACCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTaca                                                                                                                                                                                                 <  1:1006427/288‑1 (MQ=17)
                                                               aTGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAcc                                                                                                                                                                                               >  2:830938/1‑288 (MQ=255)
                                                               aTGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGAAGCTGGCGCGTCTTATCAGGTCAACAcc                                                                                                                                                                                               >  2:638154/1‑288 (MQ=255)
                                                                  tgAAGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACAcc                                                                                                                                                                                               >  2:1006427/1‑285 (MQ=255)
                                                                  tgAAGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCAGCACCACGTAGGTCAGACAAGGCGATCACGCCGCCTCCGACATCGAACGCCCGAGACGGTTGCCTGATGCGACGCCGGCGCGTCTTGTCAGGCCTCCAcc                                                                                                                                                                                               >  2:712082/1‑285 (MQ=255)
                                                                    aaGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTCTCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGCCAGACAAGGCGGTTACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATTAGACGCTGGTGCGTCTGCTGAGGCCGCAACCGCttga                                                                                                                                                                                         >  1:145741/1‑286 (MQ=18)
                                                                    aaGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGCGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCtgt                                                                                                                                                                                          >  2:84860/1‑288 (MQ=255)
                                                                            cgTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCCCTGTGAAGTGCt                                                                                                                                                                                  >  2:1068612/1‑288 (MQ=255)
                                                                                   aaGGCGGGAGATAAAATTGATGACGCGGGAGTCAGGTAAAGGCCCGGGAAGTGCGGCACCGGGTAGGCCGTCCAAGGCGGGCAGTCCGCATGCGACTGACCACGCCCGAGCGGGGTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCAcccc                                                                                                                                                                           <  1:556608/288‑1 (MQ=14)
                                                                                       cGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGtt                                                                                                                                                                                                                                      >  2:634878/1‑225 (MQ=255)
                                                                                       cGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAgg                                                                                                                                                                      >  2:721163/1‑289 (MQ=255)
                                                                                                aaaTGGACAACGCGGGAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGAAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGAGGCGACGCTGGCGCGTCTTATCAGACCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATaa                                                                                                                                                              <  2:673603/288‑1 (MQ=255)
                                                                                                                         aaCTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTa                                                                                                                                    >  2:709075/1‑289 (MQ=9)
                                                                                                                           ctcgcgggAAGGGCGGCGCCCCGTAGGGTAGACAAGGCAGTGAGGCAGCGTCGGACATCCACCGCGCGGGCCGGTTGCCGAATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAAc                                                                                                                                  <  1:830938/282‑1 (MQ=14)
                                                                                                                               gtgaaataggggcgtctgcgggcggggACAGGGTGGTCAGGCAGCGTCGGACAGCAGACGCCCGGGCAGATTGCGGGAGGCGACGCTGGCGCGTCGTAGGAGGCCTAAACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGcc                                                                                                                               <  1:302230/262‑1 (MQ=14)
                                                                                                                                       gcggacccccGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGGGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCgg                                                                                                                       <  2:77296/282‑1 (MQ=255)
                                                                                                                                                     ctcatccaacgcgtcccgcccccacccGACATCCAACACCCGGCCCGGTTTCCTGAGGCGACGCTGGCGTGTCTTATCAGGCCTACACCGCTGTGACGTGCGGCACCCCGTAGGTCAGACAGGGCGGTCACGCCGCATCCTACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTCTCAGGCCTACACCGCTGTCAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACg                                                                                                        <  2:995915/264‑1 (MQ=14)
                                                                                                                                                               gCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGATGGcgcg                                                                                                >  2:88219/1‑287 (MQ=255)
                                                                                                                                                                    cgcgcccgatgcGAAATCGAGCGCCCGAGCGGGTGGCCGAGTGGGAAGGTGGCGCGGCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACAACCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                          <  1:721163/277‑1 (MQ=14)
                                                                                                                                                                    cACGCCCCATCCGACATCCAACGCCCGAGCGGGTTGCCGGATGGGACGCTGGCGCGTCTTATCAGGCCTAAACAGCTGTGAAGGGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATc                                                                                         <  2:872496/289‑1 (MQ=255)
                                                                                                                                                                           cctCCTACATCCAACCCCCGAGCCGGTTGCCGGATGCGACGCTGGCGCGTCTTATCAGCCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAATTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTa                                                                                  <  2:125066/287‑1 (MQ=255)
                                                                                                                                                                           cATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACCCCGCTGTGAAGTTCCCCACCCCGTAGGCCGGATAAGGCGGTTACGCCGCATCCGACCTCCAACGCCCCAACCGGTTGCCCGGTGCGCCGCTGGCCCCTGTTAGCAGGCCt                                                                                   >  1:1088125/1‑288 (MQ=14)
                                                                                                                                                                             tcgcagtaccAACGACCGCGCCGGTGGCCTGCCGCGCGGCTGGCGCGTCTTACCAGGCCTACACCGCTGGGAGGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTaca                                                                                <  2:890181/281‑1 (MQ=14)
                                                                                                                                                                             tCCGACTTCCAACGCCGGAGCCGGTGGCCTGATGCGACGCTGTCGCGTCTAAGCAGGCCGAGACGGCGGTGAAGGGAGGCACCCGGTAGGTCGGACGAGGCGGTCACGCCGCATCCGACATCCAACGCCCGGGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTaca                                                                                <  1:98973/289‑1 (MQ=11)
                                                                                                                                                                             tCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTaca                                                                                <  2:106829/289‑1 (MQ=40)
                                                                                                                                                                                              gggCCGGTGGCCTAATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCCGTGAAGTGCGGCCCCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACACCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTcc                                                               <  2:52460/287‑1 (MQ=11)
                                                                                                                                                                                              gagaCGGTTGCCTGATGCGACTCTGGCGCATCTTATCAGGCCTAAACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTcc                                                               <  2:280040/289‑1 (MQ=255)
                                                                                                                                                                                                                                      ccTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGACGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCCTCCGACa                       >  2:31995/1‑289 (MQ=255)
                                                                                                                                                                                                                                       cTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGCCGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                          >  2:1008126/1‑221 (MQ=255)
                                                                                                                                                                                                                                          cacCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCACCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCa                   <  2:326154/289‑1 (MQ=255)
                                                                                                                                                                                                                                           acCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCa                   >  2:50300/1‑288 (MQ=40)
                                                                                                                                                                                                                                               cTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGTTAAGGCGGTTACGCCGCATCCGACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCCGGCCTACCCCGCTGCGCAATGCTCCACCCCCTAGCTTCGACAAAGCCGTTACGCCCAAGCCGACATcaatcgc               >  1:249514/1‑282 (MQ=11)
                                                                                                                                                                                                                                                       tGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGCTGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTCCACCGCTGTTAAGTGCCCCACCCCGTAGTTTCGATTAGGCGGCTACCCCGCCTCCCActaccacgcaccgcgccgg      >  1:164201/1‑271 (MQ=11)
                                                                                                                                                                                                                                                       tGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGt                                                                                               >  1:1101/1‑200 (MQ=255)
                                                                                                                                                                                                                                                       tGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGt                                                                                               <  2:1101/200‑1 (MQ=255)
                                                                                                                                                                                                                                                         cGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTACCAGGCCTACACCGCTGTGAAGTGCCCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGATATCCAACGTCCGATCCCGtt    >  2:938422/1‑289 (MQ=255)
                                                                                                                                                                                                                                                           gcatctgttAGGTGAGGTAACGCGGTCAGTCTGCACCGTACATTCGACGCGCGAGCCGGTGGCCCAATGCCACGGGGGCGCGGCTTAGCGGTTCTAAACCGCTGTGAAGTGCGCCACCCCTTAGGTCTGATAAGGGGGTGCCGCCGCGTCCAACATCTCCCGCCCAAGCTGGTTGCGTGGTGCAACGCTGGCGCGTCTGCTCAGGGTTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGc  <  1:211588/281‑1 (MQ=2)
                                                                                                                                                                                                                                                                 cGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                          <  1:596940/195‑1 (MQ=38)
                                                                                                                                                                                                                                                                 cGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                          >  2:596940/1‑195 (MQ=38)
                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                  
ATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGCGGAAGAGAAAATTGACAACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGC  >  NC_000913/2304270‑2304809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: