BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	701,938	T→C	3.4%	T272A (ACT→GCT)	nagA ←	N‑acetylglucosamine‑6‑phosphate deacetylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	701,938	0	T	C	3.4%	542.4 / 3.6	148	T272A (ACT→GCT)	nagA	N‑acetylglucosamine‑6‑phosphate deacetylase
Reads supporting (aligned to +/- strand): ref base T (80/63); new base C (2/3); total (82/66)
Fisher's exact test for biased strand distribution p-value = 6.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 148 total aligned reads displayed.

AATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGG‑CAAAGGTAATCCCCGCGCGGAAACCGGCT‑TTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCA  >  NC_000913/701653‑702221

                aGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTc                                                                                                                                                                                                                                                                              >  2:38498/1‑288 (MQ=255)

                                        ggcgAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTTATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACTCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGAGGGGCGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGAAGTTTAGCGTTgaga                                                                                                                                                                                                                                                     >  2:879496/1‑289 (MQ=255)

                                        ggcgAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGa                                                                                                                                                                                                                                                     >  1:677450/1‑289 (MQ=255)

                                                            tctcAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACAt                                                                                                                                                                                                                                  >  2:794935/1‑288 (MQ=255)

                                                                     ctaTCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGGTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGTTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCATTGTTGGCACCTTCTGGCGCGTTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCTTTGCGAATGTTGGCGTAATCAACATGCAGGCCATc                                                                                                                                                                                                                        <  2:694963/287‑1 (MQ=255)

                                                                     cAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATc                                                                                                                                                                                                                        <  1:718808/289‑1 (MQ=255)

                                                                       aTCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCa                                                                                                                                                                                                                       >  1:141731/1‑288 (MQ=255)

                                                                       aTCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCa                                                                                                                                                                                                                       >  2:190365/1‑288 (MQ=255)

                                                                                gcCGGATATAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAAGAc                                                                                                                                                                                                             <  2:281456/289‑1 (MQ=255)

                                                                                gcCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATAc                                                                                                                                                                                                             <  2:380552/289‑1 (MQ=255)

                                                                                 ccggagagtgctgccgcATACGTGGGACGTCATCCAGTGCGATACCGCAAGGTTCGGCCAGATTACGCACGACTTCAATCAGGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATAc                                                                                                                                                                                                             <  1:730969/272‑1 (MQ=255)

                                                                                              cGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTagc                                                                                                                                                                                                                                                                                             <  2:369098/195‑3 (MQ=255)

                                                                                              cGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTagc                                                                                                                                                                                                                                                                                             >  1:369098/1‑193 (MQ=255)

                                                                                              cGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCa                                                                                                                                                                                              >  2:712555/1‑289 (MQ=255)

                                                                                               gCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAg                                                                                                                                                                                             <  2:677450/289‑1 (MQ=255)

                                                                                                 caatagtaaaaCTTCATCAAGTGCCATAACGAAATGTCGTACCAGATCAAGCACCCCTTCGATAAGGGTTAAGGATAAAACGTTTAACATACCGTTCACATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAGATGTTTCATGTTGGAAGCTGCTGGCTCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTCGCGAATGTTGTCGTAATGATCATTCAGAGTATCAGAAATAATACC‑GCAATAAATGTGAGCt                                                                                                                                                                                           <  1:328708/279‑1 (MQ=255)

                                                                                                 cATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCt                                                                                                                                                                                           <  1:38498/289‑1 (MQ=255)

                                                                                                        aGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATAc                                                                                                                                                                                                             <  2:478934/265‑1 (MQ=255)

                                                                                                        aGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGa                                                                                                                                                                                    >  1:898732/1‑289 (MQ=255)

                                                                                                          cACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGCGGCTTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTTGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAgg                                                                                                                                                                                  >  1:967217/1‑289 (MQ=255)

                                                                                                             ttCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTCGCGTTGCGAATGTTGGCGTAATCAACCTGCCGGCCATCAGCAATCATACC‑GCAATAAATGTAAGCTTCGTCGAGGATc                                                                                                                                                                               >  1:152893/1‑289 (MQ=255)

                                                                                                             ttCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGt                                                                                                                                                                                                                                           >  1:96988/1‑230 (MQ=255)

                                                                                                             ttCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGt                                                                                                                                                                                                                                           <  2:96988/230‑1 (MQ=255)

                                                                                                             ttCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGCAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTAGTCGAGGATc                                                                                                                                                                               >  1:1009647/1‑289 (MQ=255)

                                                                                                               cATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATcgc                                                                                                                                                                             >  2:37099/1‑289 (MQ=255)

                                                                                                                aTCCAATTCGATACCGCAATGTTCGACCAGATTACGACCGCCTTCAATCATTGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATcgcg                                                                                                                                                                            <  1:270732/289‑1 (MQ=255)

                                                                                                                    aGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGcgccc                                                                                                                                                                         >  1:943828/1‑288 (MQ=255)

                                                                                                                           tACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCgccaggcc                                                                                                                                                                 >  1:586310/1‑289 (MQ=255)

                                                                                                                                     ttCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGCCGAGGATCGCGCCCCCCCGGCCAGGTTCACg                                                                                                                                                        >  1:997538/1‑288 (MQ=255)

                                                                                                                                     ttCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGCAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACg                                                                                                                                                        >  1:823928/1‑288 (MQ=255)

                                                                                                                                      tCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGAc                                                                                                                                                      <  2:283061/289‑1 (MQ=255)

                                                                                                                                      tCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGAc                                                                                                                                                      <  2:152893/289‑1 (MQ=255)

                                                                                                                                      tCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGAc                                                                                                                                                      <  1:405932/289‑1 (MQ=255)

                                                                                                                                      tCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGAc                                                                                                                                                      <  1:48809/289‑1 (MQ=255)

                                                                                                                                      tCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCGTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGCAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGAc                                                                                                                                                      <  2:557369/289‑1 (MQ=255)

                                                                                                                                      tCGACCAGATTACGCACCCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGATCACGAc                                                                                                                                                      <  2:6782/289‑1 (MQ=255)

                                                                                                                                       cGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCg                                                                                                                                                                                                                                                                             <  1:812922/170‑1 (MQ=255)

                                                                                                                                       cGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCg                                                                                                                                                                                                                                                                             >  2:812922/1‑170 (MQ=255)

                                                                                                                                       cGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGACAGGTTCAAGaac                                                                                                                                                     >  2:336124/1‑287 (MQ=255)

                                                                                                                                          ccAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGt                                                                                                                                                  >  1:102316/1‑289 (MQ=255)

                                                                                                                                          ccAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGCAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCgg                                                                                                                                                   <  2:823928/288‑1 (MQ=255)

                                                                                                                                           cAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTa                                                                                                                                                 <  2:715132/289‑1 (MQ=255)

                                                                                                                                               ttACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAatat                                                                                                                                             >  2:388133/1‑289 (MQ=255)

                                                                                                                                                tACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAAtata                                                                                                                                            <  2:1010226/289‑1 (MQ=255)

                                                                                                                                                     ccgcCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCa                                                                                                                                       <  2:434283/288‑1 (MQ=255)

                                                                                                                                                     acgcCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCa                                                                                                                                       <  1:712555/289‑1 (MQ=255)

                                                                                                                                                     acgcCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCa                                                                                                                                       <  2:1072500/289‑1 (MQ=255)

                                                                                                                                                     acccattccactggtattAAGGATCAGTCGCTTAACTTACCTTTCTCTTCCACCCATAGTCCGTTACGGTAGTATATTTTTTTACCCGCAAGAATGAACTTTTCAATTTTGCCACCTGCGGGCGCGGGGGCGTCAGTAACCAGACACAGTTTGTCGCCTTGCAGACGTTGAGCGTTGCTAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GAAAGAAATGTCAGCGTCGTTGAGGGTCGCACCCACCAGGCCAGGTTCACGACCGGTAATATACGGCa                                                                                                                                       <  1:879496/273‑1 (MQ=255)

                                                                                                                                                       gcCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATc                                                                                                                                     <  1:190365/289‑1 (MQ=255)

                                                                                                                                                        ccTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATc                                                                                                                                     >  2:595454/1‑288 (MQ=255)

                                                                                                                                                          ttCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATcgcg                                                                                                                                  <  2:182831/289‑1 (MQ=255)

                                                                                                                                                                   ggTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATcgcg                                                                                                                                                                            <  2:114355/238‑1 (MQ=255)

                                                                                                                                                                   ggTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGa                                                                                                                         >  1:137633/1‑289 (MQ=255)

                                                                                                                                                                   ggTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGa                                                                                                                         >  2:778735/1‑289 (MQ=255)

                                                                                                                                                                   ggTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGa                                                                                                                         >  2:258757/1‑289 (MQ=255)

                                                                                                                                                                   ggTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACAAAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAACTGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGAAACAGTTTGTCGCTTTTCAGACGTTTAGCGTTGCGAAGGTTGGCGTAATCAACATGCAGGCCATCAGTAATAATACC‑GCACTAAATTTCAGACTCGTCGAGGACCGAGCCGGCCAGGCCAGGTTCACGACCGGTAATTTACGGCATCGCGTTGTCCa                                                                                                                           >  1:634170/1‑287 (MQ=255)

                                                                                                                                                                         ggATGCACCGCTTAAAATACCGTTCTCATCCACACAAAGTCCGTCACGGTAGTATATTGTTTTACCCGCAAAAATGAAATCTTCAATGTTGGCACCGGCTGGCGCGGTGGCGTCAGTAACAAGCCACAGTTTGTCGCCTTTCATACGTTTAGCTTTGCGAATGTTTGCGTAATCAACATGCAGGCCATCAACAATGATACC‑GCAATAAATGTCCGCTTAGTCGAGGATAGTGAACGCAAGCCCAGGTTCACGACCGTTAATACACGGccgtcgctgtacggctaggtgg                                                                                                                   >  1:947052/1‑268 (MQ=255)

                                                                                                                                                                            tGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGG‑Ca                                                                                                               <  2:137633/289‑1 (MQ=255)

                                                                                                                                                                            tGAACCCCTTCACGTACCTTTCTCATCCACACAAAGTCCGTTACGGGCGTAAATGGTTTTACCCGCAAAAATGAACTGTTCTATGTTGGCACCAGCTGGCGCGGTGGCGTCAGTAACCAGACTCAGTTTGTCGCCTTTCAGACGTTTAGCGTTGGGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAAGACC‑GCATTAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGG‑Ca                                                                                                               <  1:290844/289‑1 (MQ=255)

                                                                                                                                                                               aCCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCCAGACCGGTCATATACGGCATCGCGTTGTACAGATGGGTGG‑CAAAg                                                                                                            >  1:546585/1‑289 (MQ=255)

                                                                                                                                                                                           aCCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCt                                                                                                                                                                                           <  2:555662/199‑1 (MQ=255)

                                                                                                                                                                                             cGTTCTCATCCACACAACGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATAAGGCATCGCGTTGTGCAGAAGGGTGG‑CAAGAGTAATCCCcgcgag                                                                                              >  1:864696/1‑287 (MQ=255)

                                                                                                                                                                                              gTTCTCACCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGG‑CAAAGGTAATCCCCGCGCgg                                                                                             >  2:25255/1‑289 (MQ=255)

                                                                                                                                                                                                  tcATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCTTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCACCGCGTTGTACAGATGGGTGG‑AAAAGGTAATCCCCGCGCGGaaa                                                                                          >  2:82218/1‑288 (MQ=255)

                                                                                                                                                                                                         cacaAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACg                                                                                                                                                        <  2:934058/220‑1 (MQ=255)

                                                                                                                                                                                                         cacaAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCACGGTTCACGACCGGTAATAAACGGCTACCCGTTGTTCAGATGGGTGG‑ACAAGGTACTCCCCGCGACGAAACCGGCt‑tt                                                                                 >  1:910735/1‑289 (MQ=255)

                                                                                                                                                                                                              cgggtaaaacagtagtaTATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCCTTGTACACATGGGTGG‑CAAAGGTAATCCCCGACCGGAACCCGGCT‑TTTGctt                                                                            >  1:65849/12‑289 (MQ=255)

                                                                                                                                                                                                                       cGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGG‑CAAAGGTAATCCCCGCGCGGAAACCGGCT‑TTTGCTTCTTTCAACg                                                                   <  2:410909/289‑1 (MQ=255)

                                                                                                                                                                                                                       cGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGG‑CAAAGGTAATCCCCGCGCGGAAACCGGCT‑TTTGCTTCTTTCAACg                                                                   <  2:102316/289‑1 (MQ=255)

                                                                                                                                                                                                                       cGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAAGTTTGGCACCGGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGG‑CAAAGGTAATCCCCGCGCGGAAACCGGCT‑TTTGCTTCTTTCAACg                                                                   <  1:398107/289‑1 (MQ=255)

                                                                                                                                                                                                                          tagtaTATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGG‑CAAAGGTAATCCCCGCGCGGAAACCGGCT‑TTTGCTTCTTTCAACGTc                                                                 >  1:890340/1‑288 (MQ=255)

                                                                                                                                                                                                                            gtaTATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTACTGGCGCGGTGGCTTCAGTAACCAGACACAGTTTGTCGCCGTTTAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGAACGCGCCCGCCAGGCCCAGTTCACGACCGGTAATATACGGCATACTGTTGTACAGATGTGCGG‑CAAAGGTAATCCCAGCGCGGACACCGGAT‑TAGTGTTGTTTTAACGTcgcg                                                              >  1:835833/1‑289 (MQ=255)

                                                                                                                                                                                                                                  tGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTAAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCCATAATACA‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGG‑CAAAGGTAATCCCCGCGCGGACACCGGCT‑TTTGCTTCTTTCAACGTCGCGTTGGAg                                                        >  2:295252/1‑289 (MQ=255)

                                                                                                                                                                                                                                    ttttACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGG‑CAAAGGTAATCCCCGCGCGGAAACCGGCT‑TTTGCTTCTTTCAACGTCGCGTTGGAGTg                                                      >  2:379463/1‑289 (MQ=255)

                                                                                                                                                                                                                                      ttACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTAAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAACTGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGACAGGTTCACGACCGGTAATATAAGGCATCGAGATGTACAGATGGGTGG‑CAAAGGTAATCCCCGCGCGGAAACCGGCT‑TTTGCTTCTTTCAACGCCCCGTTGGAGTGa                                                     >  2:869262/1‑288 (MQ=255)

                                                                                                                                                                                                                                       tACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGG‑CAAAGGTAATCCCCGCGCGGaaa                                                                                          >  1:782100/1‑251 (MQ=255)

                                                                                                                                                                                                                                       tACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGG‑CAAAGGTAATCCCCGCGCGGAAACCGGCT‑TTTGCTTCTTTCAACGTCGCTTTGGGTTGAcc                                                   >  1:1099193/1‑289 (MQ=255)

                                                                                                                                                                                                                                       tACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGG‑CAAAGGTAATCCCCGCGCGGAAACCGGCC‑TTTGCTTTTTTTAACGTCGCCTTGGAGTGAcc                                                   >  1:6970/1‑289 (MQ=255)

                                                                                                                                                                                                                                       tACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGG‑CAAAGGTAATCCCCACGCGGAAACCGGCT‑TTTGCTTCTTTCCACGTTGCGTTGGAggtacc                                                   >  1:234734/1‑284 (MQ=255)

                                                                                                                                                                                                                                       tACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGT‑ATTAACATTCCGGCCATCAAGAAAAATTCCGGCATAAAATGT‑AACCTCCTCCAAGAATGCCGCCGCCAGGCCAGGTTCCCGACCGGTAATATTCCGCACTGGGTTGTACAGATGGGTGGCCAAAGGTAA‑CCCCGCGCGGAAAACGGGTGTTTCCTTCTTCCAACTCCCCGTTgggtgaacc                                                   >  1:1005147/1‑282 (MQ=255)

                                                                                                                                                                                                                                         cccGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGc                                                                                                                                                                                                                                             >  2:354552/1‑104 (MQ=255)

                                                                                                                                                                                                                                         cccGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCgg                                                                                                                                                   >  2:90901/1‑193 (MQ=255)

                                                                                                                                                                                                                                         cccGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCgg                                                                                                                                                   <  1:90901/193‑1 (MQ=255)

                                                                                                                                                                                                                                            gCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGG‑CAAAGGTAATCCCCGCGCGGAAACCGGCT‑TTTGCTTCTTTCAACGTCGCGTTGTAGTGACCGGCAg                                              >  1:762752/1‑289 (MQ=255)

                                                                                                                                                                                                                                            gCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGG‑CAAAGGTAATCCCCGCGCGGAAACCGGCT‑TTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAg                                              >  1:155492/1‑289 (MQ=255)

                                                                                                                                                                                                                                              aaaaaTGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACAAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTACTCAACATGCAGGCCATCAGAAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGG‑CAAAGGTAATCCCCGCGCGGAAACCGGCT‑TTTGCTTCTTTCAACGTCGCGTAGGAGTGCCCGGCAGaa                                            >  2:577561/1‑289 (MQ=255)

                                                                                                                                                                                                                                                  aTGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGG‑CAAAGGTAATCCCCGCGCGGAAACCGGCT‑TTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACca                                        <  1:258757/289‑1 (MQ=255)

                                                                                                                                                                                                                                                          ttCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGG‑CAAAGGTAATCCCCGCGCGGAAACCGGCT‑TTTGCTTCTTTCa                                                                      >  1:458341/1‑251 (MQ=255)

                                                                                                                                                                                                                                                          ttCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGG‑CAAAGGTAATCCCCGCGCGGAAACCGGCT‑TTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCg                                >  2:890539/1‑289 (MQ=255)

                                                                                                                                                                                                                                                          ttCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACA‑ATGGGTGG‑CAAAGGTAATCCCCGCGAGGAAACCGGCT‑TTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAAACACAATCCCg                                >  2:694238/1‑288 (MQ=255)

                                                                                                                                                                                                                                                                  tGGCACCTGCTGGCGCTGTTGCGGCAGTAACCAGACACAGTTTGTCACCTTTGAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGTATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGG‑CAAAGGTAATCCCCGCGCGGAAACCGGCT‑TTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGc                        <  2:909459/289‑1 (MQ=255)

                                                                                                                                                                                                                                                                    gCACCTGCTGGCGCGGTGGCGTCAGTAACCAGacac                                                                                                                                                                                                                                                                                      >  1:363841/1‑36 (MQ=255)

                                                                                                                                                                                                                                                                    gCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTGACGACCGGTAATAAACGGCATCGCGTTGTACAGATGGGTGG‑CAAAGGTAATCCCCGCGCGGAAACCGTCT‑TTTGCTTCTTTGCACGTCGCGGTGGAGTGACCGGCGCAAACCACAAACCCGGCAttctcca                      >  1:916498/1‑284 (MQ=255)

                                                                                                                                                                                                                                                                      aCCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGG‑CAAAGGTAATCCCCGCGCGGAAACCGGCT‑TTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGt                    <  1:379463/289‑1 (MQ=255)

                                                                                                                                                                                                                                                                         tgctgGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGG‑CAAAGGTAATCCCCGCGCGGAAACCGGCT‑TTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAGACCACAATCCCGGCATTTGCCAGttt                  >  2:363333/1‑288 (MQ=255)

                                                                                                                                                                                                                                                                          gctgGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTAAGACGTTTAGCGTTGCGAATGTTGGCGTAATCGACATGCAGGCCATCAGCAGTAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTGGTACAGAGGGGTGG‑CAAAGGTAATCCCAGCGCGGAACCCGGCT‑TTTGCCTCTTTCAACGTCGCATTGGAGTGACCGGCAGCAACCACAAGCCGGGCAtttcgccgttt                  >  1:109837/1‑279 (MQ=255)

                                                                                                                                                                                                                                                                               cgcgGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGG‑CAAAGGTAATCCCCGCGCGGAAACCGGCT‑TTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCtgatg           >  2:751982/1‑289 (MQ=255)

                                                                                                                                                                                                                                                                                    tGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGG‑CAAAGGTAATCCCCGCGCGGAAACCGGCT‑TTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACtt       >  1:472154/1‑288 (MQ=255)

                                                                                                                                                                                                                                                                                        gTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGG‑CAAAGGTAATCCCCGCGCGGAAACCGGCT‑TTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGc   <  2:890340/288‑1 (MQ=255)

                                                                                                                                                                                                                                                                                        gTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGG‑CAAAGGTAATCCCCGCGCGGAAACCGGCT‑TTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCa  <  1:595454/289‑1 (MQ=255)

AATCAGGTGTGAATGCAGTCAGGTTGGCTACTTTACCTGCGGCGAGTGTGCCGAGACGTTTCTCAACGCCAATCGCACGCGCCGGATAGAGCGTCGCCATACGTAGCACTTCATCCAGTGCGATACCGCAATGTTCGACCAGATTACGCACGCCTTCAATCATGGTTAAGGATGAACCGCTTAACGTACCGTTCTCATCCACACAAAGTCCGTTACGGTAGTATATTGTTTTACCCGCAAAAATGAACTGTTCAATGTTGGCACCTGCTGGCGCGGTGGCGTCAGTAACCAGACACAGTTTGTCGCCTTTCAGACGTTTAGCGTTGCGAATGTTGGCGTAATCAACATGCAGGCCATCAGCAATAATACC‑GCAATAAATGTCAGCTTCGTCGAGGATCGCGCCCGCCAGGCCAGGTTCACGACCGGTAATATACGGCATCGCGTTGTACAGATGGGTGG‑CAAAGGTAATCCCCGCGCGGAAACCGGCT‑TTTGCTTCTTTCAACGTCGCGTTGGAGTGACCGGCAGAAACCACAATCCCGGCATTTGCCAGTTTGCTGATGACTTCCGCA  >  NC_000913/701653‑702221

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`