Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,304,799 G→A 7.7% intergenic (+408/+307) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,304,7990GA 7.7% 113.3 / 2.2 50intergenic (+408/+307)eco/mqoecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base G (22/22);  new base A (2/2);  total (25/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.36e-01

GAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCAT  >  NC_000913/2304513‑2305066
                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                           
gAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGa                                                                                                                                                                                                                                                                            <  2:736421/288‑1 (MQ=255)
    tGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACCTCCAACGTCCGATCCgg                                                                                                                                                                                                                                                                       >  2:429480/1‑289 (MQ=255)
         cACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGCAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTg                                                                                                                                                                                                                                                                    >  2:394916/1‑287 (MQ=39)
           ccccGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGACTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGGTACGCCGCATCCGACATCCAACGTCGGAGCCGGTTGGCTg                                                                                                                                                                                                                                                                >  2:771427/1‑289 (MQ=255)
              cGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTAAGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATg                                                                                                                                                                                                                                                             >  2:328134/1‑289 (MQ=37)
                        aCAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGgc                                                                                                                                                                                                                                                   >  2:29886/1‑289 (MQ=37)
                        aCAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAACCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCCCACGTCCCAGCCGGTTCCCTGacgagtcccggcc                                                                                                                                                                                                                                                   >  1:766565/1‑277 (MQ=255)
                                  acgccggcctcggccgccgacggacctggCCGTTTGCCGCATGGGCCGCTGGCGCGTCGTATCAGCCGTCCACGGCGGGTAAGTGCGCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCAAAGCCGGTGGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                                                                                                                                                                          <  1:471294/260‑1 (MQ=11)
                                  aaggcccctggcccgaccgaaCGCCCGGGCGGGTGGCCTGATGCGACGCTGGCGCGTCTTAGCGGGGCTAGACGTCGGTGAAGTGCTCCACCCCGTAGTTCGGATAAGGCGGTCACGCCGCATCCGACATCGAACGCCCGAGCCGGTTGCCTGAGGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                                                                                                                                                                          <  1:295179/269‑1 (MQ=11)
                                          atgcccaggcaagggcgcgggtgggTGGCGTGATGCGACGCGGGGGCGTCTTATCAGGCCTAGACCGCGGTGAAGTGCTGCACCCCGTGGGTCGGATAAGGCGGATGCGCCGCATCCGACATCTAACGCCCGAGCCGGTGGCGTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTa                                                                                                                                                                                                                                  <  1:533325/265‑1 (MQ=11)
                                                                       cTGAGGCGACGTTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAgg                                                                                                                                                                                                     <  2:807599/288‑1 (MQ=255)
                                                                                        caccTTATCAGCCCTAGACCGCTTTTAAGTGCGCCACCCCGTAGGTCAGGTAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTTCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTAcg                                                                                                                                                                                   <  2:568366/286‑1 (MQ=255)
                                                                                                    ccgagagcgctgtcaatgtctgaaCCCCGGGGGTCGGTGCAGGCGTTCACGCCGCAGCGCACATCGAACGCCCGAGCGGGTTGCCTGATGGGACGCTGGCTCGTCTTATCAGGACTACACGGCTGTGAAGTGCTGCACCCCGTAGGTGGGATAAGGCGGTGACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGa                                                                                                                                                                         <  1:686764/264‑1 (MQ=11)
                                                                                                                          cACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTCCACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCCCATCCGACCTCCACCGCCCGACCAGGTTGCCTGCTGCGACGCTGGCGCGTCTTATCCGCCCTACCCCGCTGCGAAGTACCGCCCCCCGTCGGCCGGCTCAGGCTGCTACCCCGCCTCCCACAGCCAACGCCCAAGACgcgtcc                                                                                                                                                  >  1:659558/1‑283 (MQ=14)
                                                                                                                               cggaGGTCGGATAAGGCCGTCACGCCGCATCCGCCAGCTAACCCCCGAGCCGGTGGCCGGAGGCGAAGCTGGCGCGGCGTATCAGGCCTAAACCGCTGTGAAGTGCGCCACCCCGTAGGTCGGATAAGGCGGTCACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCCCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATg                                                                                                                                            <  2:700267/286‑1 (MQ=11)
                                                                                                                               cGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCTCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACACATAACGCCCAAGCCGGttcactcat                                                                                                                                             >  2:246933/1‑281 (MQ=255)
                                                                                                                               cGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGt                                                                                                                                                                                                                                               >  2:324715/1‑190 (MQ=35)
                                                                                                                               cGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGAt                                                                                                                                             >  2:661687/1‑288 (MQ=255)
                                                                                                                                 tAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGCTGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCACACACCCAACCACCGAGCCGGTTGCCTGATGCCACGCTGGCCCGTCCTATCATGCCACCACCGCTTCGGCGTGCTCCACCCCGTAGGGCGGATAGGGCGCTTACGCCACATCCGACGTCGCACCCCACAGCCGGTTGCCTGacgg                                                                                                                                           >  1:284680/1‑285 (MQ=14)
                                                                                                                                 tAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGGCGGATAAGGCGGTTACGCCGCATCCGACATCTAACCCCCAAGCCGGTTGCCTGAtctg                                                                                                                                          >  2:100992/1‑286 (MQ=255)
                                                                                                                                              gCGGTTCCGCCTGATCCGGCTTCGAACACCCAATCCGGTTCCCTGATGCGAGGCGGGCGCGTCTGATCAGGCCTACCCCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGt                                                                                                                              <  1:394916/288‑1 (MQ=11)
                                                                                                                                              gCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTTCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAACACGGTTGCCTGATGCGACGCTGgctct                                                                                                                               >  2:734786/1‑284 (MQ=255)
                                                                                                                                              gCGGTTACGCCGCACCCGCAATCTTGCCCCCGAGCCGGTGGGCGAATGCGGCGCTGGCCGGTCTGTCGAGGCCTACACGGCTGTGATGTGCTCCACCCGGGAGGTGGGATAAGGCGGTTACGCCGCATCCGACATCCAACGCCCGGGCCGGTTGCCGGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGt                                                                                                                              <  1:726228/288‑1 (MQ=11)
                                                                                                                                                  ttACGCCGCATCCGACATCTAACGCCCAGGCCGGTGTCCTGATGCGACGCGGGCGCGTCTGATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                                                         <  2:413644/289‑1 (MQ=255)
                                                                                                                                                   tACGCCGCTTCGGACATCGACCGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATc                                                                                                                        <  2:494656/289‑1 (MQ=255)
                                                                                                                                                   tACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCAcccc                                                                                                                                                                                                          >  2:564107/1‑207 (MQ=11)
                                                                                                                                                   tACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCAcccc                                                                                                                                                                                                          <  1:564107/207‑1 (MQ=11)
                                                                                                                                                   tACACCGCACCCGACGTGGTACGCCCAGCCGGGCTGCCTGCAGCTCCGCTGGCGCGGCTTCGCAGGCCGACACCGCTGTGAAGTGGTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAGCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                                                         <  1:29886/288‑1 (MQ=11)
                                                                                                                                                            gccgacatgcgaggcccggtgcGGTTGGCTGCAGCGCCGCTTGCGCGTCTGATCATGGCTACACCGTTGTGCAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGCCCGAGCCTGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTaca                                                                                                               <  1:328134/268‑1 (MQ=11)
                                                                                                                                                             gcgtcgccTAACGCCGCGGCCGGTTTGGTGAGGCGCCGCTGGGGCGGCTTATCGGTCCTACACCGCTGTGACGGTCGCCACCCCGGAGGTGGGTGAAGGCGGTTACGCCGCAGCCGACATCCAACGCGCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCTTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTaca                                                                                                               <  1:771427/281‑1 (MQ=11)
                                                                                                                                                                    gcgaCGCCCAGGCCGGTGGCCGGAGAGAACGTGGGCGGGTCTGATCAGGCCTAGACCCCTGTGAATGGCGCCACCCCGTAGGTCAGATAAGGCGGTGAAGCCCAATCCGAAATCTATCCCCCAGTCCGGTGGCCGGATGCGACGCTGGCGCGTCTTATCTGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCtgtg                                                                                                       <  1:169991/286‑1 (MQ=11)
                                                                                                                                                                          cccAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                                                         <  2:346151/265‑1 (MQ=40)
                                                                                                                                                                                    ttGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCCAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTaca                                                                                                               >  1:346151/1‑265 (MQ=37)
                                                                                                                                                                                     tGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTgg                                                                                                                                                                                                                                                    <  1:540806/131‑1 (MQ=37)
                                                                                                                                                                                     tGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTgg                                                                                                                                                                                                                                                    >  2:540806/1‑131 (MQ=37)
                                                                                                                                                                                                 cGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACCCCCAAGCCGGTTGCCTGATGCGACTCTAGCCCGTCTTATCCGGCCCACACCGCTGCGAAGTGCCCCAACCCGTTGGTTCGATCAg                                                                           >  1:494656/1‑288 (MQ=11)
                                                                                                                                                                                                 cGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCCGGCCTACACCGCTGTGCAGTGCCCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATGCGACATCTATCGCCCCAGCCGGTTGCCTGACGCCACCCTGGCCCGTCCTATCAGGCCACCCCCGCTGTGCCGTGccctcccacacagtctcgatactt                                                                          >  1:54896/1‑266 (MQ=11)
                                                                                                                                                                                                      gcgcGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACCTCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCCTATCAGGCCCACCCCCCTGCTACTTCCTCCAcccctgacgttcgctcacgcgctt                                                                     >  1:167018/1‑269 (MQ=11)
                                                                                                                                                                                                         cgTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCa                                                                                                                                                            <  1:13757/199‑1 (MQ=40)
                                                                                                                                                                                                         cgTCTTACCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCa                                                                                                                                                            >  2:13757/1‑199 (MQ=255)
                                                                                                                                                                                                                                 tgAAGTGCTCCGCCCCGTAGGTTGGATAAGGCGGTTACGCCTCATCCGACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCg                                           <  2:766565/288‑1 (MQ=255)
                                                                                                                                                                                                                                        cTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGgcg                                                                                                                                                                                                                                                  >  2:523474/1‑82 (MQ=255)
                                                                                                                                                                                                                                        cTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGACTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCTTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCGGCATCCAACATCTAACGCCCGAGCCGGtt                                   >  2:178047/1‑289 (MQ=255)
                                                                                                                                                                                                                                                        ggATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTTGGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGACGCGCCGCtt                   >  1:618560/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                   ttACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATg                                                                                                                                                                                                                                                             <  2:607907/44‑1 (MQ=255)
                                                                                                                                                                                                                                                                        gcctaTCCGGCACCCACGGCCCTAGCCGGTTGACTGAGTGGACGCTGCAGGGTGTTGACAGGCCGACACCGCGGTGAAGGGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGCCATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGGGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCa   <  1:429480/285‑1 (MQ=11)
                                                                                                                                                                                                                                                                         cgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCAt  >  2:497996/1‑289 (MQ=18)
                                                                                                                                                                                                                                                                         cgcATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                                                         <  2:105688/170‑1 (MQ=31)
                                                                                                                                                                                                                                                                          gcATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGACGTGCTCCACCCCGTAGGTTGGATAACGCGGATACGCCGCCTCCAAGATCTAACCCCCGACGCGGTTGCCTGATCACACGCTGGCGCCTCTTacccgtct  >  1:335116/1‑281 (MQ=11)
                                                                                                                                                                                                                                                                             tCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACg                                                                                                                                                                                                                                                        <  2:809278/39‑1 (MQ=40)
                                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                           
GAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCAT  >  NC_000913/2304513‑2305066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: