Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,996,542 Δ1 bp 4.0% coding (291/723 nt) sdiA ← quorum‑sensing transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,996,5420A. 4.0% 365.2 / 6.1 100coding (291/723 nt)sdiAquorum‑sensing transcriptional activator
Reads supporting (aligned to +/- strand):  ref base A (44/50);  new base . (2/2);  total (48/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.16e-01

Only 100 of 101 total aligned reads displayed.

CCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACATAACGAATAGTAATCGTACTCCAGCTGCTGAGCCTGAAGCTCAATTTCATGGTAGACCTCTTCTGCGGTCTCCATCCTCTGAAAACGCAACAGCATCGTGCGACGCCAGCTGAAAAAATCCTTATCCTG  >  NC_000913/1996259‑1996829
                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                               
ccgTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCAtt                                                                                                                                                                                                                                                                                            <  1:678326/289‑1 (MQ=255)
   tCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTcc                                                                                                                                                                                                                                                                                          >  2:826350/1‑288 (MQ=255)
        cTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATCACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTa                                                                                                                                                                                                                                                                                    >  2:862541/1‑289 (MQ=255)
               tttCTTTTTCTCGCTTGCTGAAATTCGCCTCTGGCGTCTTCACTATTTCAGCATGTAAACGCATCACAGCCATCAGACGTGCGCGCACCAGTAGCTGCATTTTTAATTGCATTTCAGCACTACGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAATCCCAGTGCCCGGGTTGGTAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGcc                                                                                                                                                                                                                                                                             <  1:810409/289‑1 (MQ=255)
               tttCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGcc                                                                                                                                                                                                                                                                             <  1:933401/289‑1 (MQ=255)
                  cTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTaaa                                                                                                                                                                                                                                                                                  >  1:798834/1‑281 (MQ=255)
                  cTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTaaa                                                                                                                                                                                                                                                                                  <  2:798834/281‑1 (MQ=255)
                  cTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCACAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACGGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCCTCATTCCACATTAAATGGCGCTg                                                                                                                                                                                                                                                                          >  1:349173/1‑289 (MQ=255)
                   tttttCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGa                                                                                                                                                                                                                                                                         <  2:697059/289‑1 (MQ=255)
                        cccgcTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTaaa                                                                                                                                                                                                                                                                    <  1:671862/287‑1 (MQ=255)
                         gcgaTTTCTGAAATTCGTCTCTGGCGTCAAACCTATTTCAGCATTTAAACGCATCAGAGCAATCAGACGTTCGGGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGTGTATTGCGCGCGCGCTGCAACGGGAAAAGGACAAACAGCCCTGCGCCCGGTTTGGCAGCATTAAATACTGAGGGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTaaa                                                                                                                                                                                                                                                                    <  1:667733/288‑1 (MQ=255)
                                    aaTTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGAACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGtt                                                                                                                                                                                                                                                        <  1:774661/289‑1 (MQ=255)
                                     aTTCATTTCTGGGGTCATCACAATTTAATCATTTAAACGCATCAGGGCCATCAGACTTGCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGAAAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTc                                                                                                                                                                                                                                                       <  1:279812/289‑1 (MQ=255)
                                          tctcTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCAc                                                                                                                                                                                                                                                  <  2:140742/289‑1 (MQ=255)
                                               ggCTTCATCCCTATTTCACCATTTAAACGAAACAGAGCCATCAGACTTCCCCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGAt                                                                                                                                                                                                                                             <  1:750089/289‑1 (MQ=255)
                                                gCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGATGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAAGGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATc                                                                                                                                                                                                                                            >  2:461249/1‑289 (MQ=255)
                                                     atcaCTATTTTATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAAtt                                                                                                                                                                                                                                        >  2:864044/1‑288 (MQ=255)
                                                     atcaCTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCCAATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGACTAATTg                                                                                                                                                                                                                                       >  1:837724/1‑289 (MQ=255)
                                                               catcatTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCa                                                                                                                                                                                                                                                             <  1:804997/257‑1 (MQ=255)
                                                               catcatTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCa                                                                                                                                                                                                                                                             >  2:804997/1‑257 (MQ=255)
                                                                 tcatTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCca                                                                                                                                                                                                                                                                                         >  2:393496/1‑227 (MQ=255)
                                                                 tcatTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCca                                                                                                                                                                                                                                                                                         <  1:393496/227‑1 (MQ=255)
                                                                 tcatTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGtttt                                                                                                                                                                                                                           <  1:438685/289‑1 (MQ=255)
                                                                     ttAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTg                                                                                                                                                                                                                        >  2:840332/1‑288 (MQ=255)
                                                                     ttAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGc                                                                                                                                                                                                                       <  2:550028/289‑1 (MQ=255)
                                                                     ttAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGc                                                                                                                                                                                                                       <  1:864044/289‑1 (MQ=255)
                                                                          cacagcAGAGCCATCAGACTTTAGGGCACCAGTAATTGCACGTTTAATTCGAGTGCAACACTAAGCAGGGGTATTTCGCGCGGGCTGCACCGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACGCCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGat                                                                                                                                                                                                                  <  1:688499/284‑1 (MQ=255)
                                                                          cGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATCCTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGat                                                                                                                                                                                                                  <  2:699094/289‑1 (MQ=255)
                                                                              tCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGTGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGataata                                                                                                                                                                                                              >  1:871787/1‑289 (MQ=255)
                                                                               cAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGataata                                                                                                                                                                                                              >  2:355617/1‑288 (MQ=255)
                                                                                       tCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCa                                                                                                                                                                                                                                                             >  1:964041/1‑233 (MQ=255)
                                                                                       tCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCa                                                                                                                                                                                                                                                             <  2:964041/233‑1 (MQ=255)
                                                                                       tCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAcccc                                                                                                                                                                                                      >  1:928333/1‑288 (MQ=255)
                                                                                       tCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCa                                                                                                                                                                                                     <  1:840332/289‑1 (MQ=255)
                                                                                       tCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCa                                                                                                                                                                                                     <  1:941781/289‑1 (MQ=255)
                                                                                            cTTTAGCGCACCAGGAACTGCATTTTTAATTGCAGGTCATCCCTAAGAATGGGTATTTCGCGAGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCTGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCt                                                                                                                                                                                                <  1:669782/289‑1 (MQ=255)
                                                                                                      ccAGTAACTGCGGTTTTAATTTCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGTAAAAGGACAAAAAGCCCAGCGCCCGGTTTTGCAGCAGTAAATACTAAGTGATACCGCGGCGGAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTACTTCGCTGAATAAGTCATCATTCCACAATAAATGGCCCTGACTAAAGTTGTCAGGGTGCAGAACCGGATCAATTGCGAGAAAGTTTTTAGCCTGATAATAACTAACCCACGCCTCAGGGTAAtt                                                                                                                                                                                      <  1:922276/289‑1 (MQ=255)
                                                                                                                 aTTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAg                                                                                                                                                                           >  1:843403/1‑289 (MQ=255)
                                                                                                                   ttttAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGcc                                                                                                                                                                         >  2:685914/1‑289 (MQ=255)
                                                                                                                                    cccTAAGAATGGGTCTTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTTATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGaa                                                                                                                                                        <  2:582312/287‑1 (MQ=255)
                                                                                                                                    caCTAAGAATGGGTATTTCGCGCGAGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTTACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCTCGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGaa                                                                                                                                                        <  2:349173/289‑1 (MQ=255)
                                                                                                                                         aGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGTGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGAACCGGATCAATTGCGAGAAGGTTTTTTGCCTTATAATAAATAACCAACGCCTCAGGGTAATTGGTGTTAAAAGCCCCTTTAGTTCGATTGAAGGGt                                                                                                                                                    >  1:485985/1‑288 (MQ=255)
                                                                                                                                              gggTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGaa                                                                                                                                                        >  1:778242/1‑279 (MQ=255)
                                                                                                                                              gggTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGaa                                                                                                                                                        <  2:778242/279‑1 (MQ=255)
                                                                                                                                              gggTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCggg                                                                                                                                              >  2:146857/1‑289 (MQ=255)
                                                                                                                                              gggTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCgg                                                                                                                                               <  2:707197/288‑1 (MQ=255)
                                                                                                                                              gggTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCgg                                                                                                                                               >  1:707197/1‑288 (MQ=255)
                                                                                                                                                 tATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTaaaaa                                                                                                                                                                            <  1:726472/256‑1 (MQ=255)
                                                                                                                                                 tATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTaaaaa                                                                                                                                                                            >  2:726472/1‑256 (MQ=255)
                                                                                                                                                     tCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCTCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAg                                                                                                                                                                           >  2:865779/1‑252 (MQ=255)
                                                                                                                                                     tCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCTCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAg                                                                                                                                                                           <  1:865779/252‑1 (MQ=255)
                                                                                                                                                        cgcgcgcTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTc                                                                                                                                                                                               <  1:79779/230‑1 (MQ=255)
                                                                                                                                                        cgcgcgcTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTc                                                                                                                                                                                               >  2:79779/1‑230 (MQ=255)
                                                                                                                                                         gcgcgcTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGAcaca                                                                                                                                   >  2:583744/1‑289 (MQ=255)
                                                                                                                                                         gcgcgcTGAAACGGCAAAAGGACAAAAAGCCCATCGCCCGGTTTGGCAGCATTAACTACTTGGTGACACCGCGGCGTAAACCATGTGCTCGCGCGGCTTCCCATAACGGCTGTGCGTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGTCTGATAATAACTAACCCTCGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGacac                                                                                                                                    <  2:885891/288‑1 (MQ=255)
                                                                                                                                                                   aCGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACATAACGAATAg                                                                                                                         <  2:159545/289‑1 (MQ=255)
                                                                                                                                                                           aGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACATAACGAATAGTAATCGt                                                                                                                  >  2:247526/1‑288 (MQ=255)
                                                                                                                                                                                                   ttGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACATAACGAATAGTAATCGTACTCCAGCTGCTGAGCCTGAAGCt                                                                                          <  2:837724/288‑1 (MQ=255)
                                                                                                                                                                                                           aTTAAATACTGAGTGAAACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACATAACGAATAGTAATCGTACTCCAGCTGCTGAGCCTGAAGCTCAATTTCa                                                                                  <  1:146857/288‑1 (MQ=255)
                                                                                                                                                                                                            ttAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACATAACGAATAGTAATCGTACTCCAGCTGCTGAGCCTGAAGCTCAATTCCATg                                                                                >  2:343756/1‑289 (MQ=255)
                                                                                                                                                                                                                  aCTGAGTGACACCGCGTCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACATAACGAATAGTAATCGTACTCCAGCTGCTGAGCCTGAAGCTCAATTTCATGGTAGAc                                                                          <  1:776960/289‑1 (MQ=255)
                                                                                                                                                                                                                     gAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCg                                                                                                                                         <  1:61078/223‑1 (MQ=255)
                                                                                                                                                                                                                     gAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCg                                                                                                                                         >  2:61078/1‑223 (MQ=255)
                                                                                                                                                                                                                     gAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACATAACGAATAGTAATCGTACTCCAGCTGCTGAGCCTGAAGCTCAATTTCATGGTAGAcc                                                                         >  2:500695/1‑287 (MQ=255)
                                                                                                                                                                                                                      aGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGaa                                                                                                                                                        <  1:190979/207‑1 (MQ=255)
                                                                                                                                                                                                                      aGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGaa                                                                                                                                                        >  2:190979/1‑207 (MQ=255)
                                                                                                                                                                                                                      aGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACATAACGAATAGTAATCGTACTCCAGCTGCTGAGCCTGAAGCTCAATTTCATGGTAGACctct                                                                      >  1:523765/1‑289 (MQ=255)
                                                                                                                                                                                                                      aGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACATAACGAATAGTAATCGTACTCCAGCTGCTGAGCCTGAAGCTCAATTTCATGGTAGACctct                                                                      >  2:645799/1‑289 (MQ=255)
                                                                                                                                                                                                                      aGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACATAACGAATAGTAATCGTACTCCAGCTGCTGAGCCTGAAGCTAAATTTCATGGTAGACctct                                                                      >  2:244819/1‑289 (MQ=255)
                                                                                                                                                                                                                       gTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACATAACGAATAGTAATCGTACTCCAGCTGCTGAGCCTGAAGCTCAATTTCATGGTAGACctct                                                                      >  2:114811/1‑288 (MQ=255)
                                                                                                                                                                                                                           cacCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCCTCATTCCACATTACATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATGGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGAACCATAACGAAGAGGAATCGTACTCCAGCTGCTGAGCCTGAAGCCCAATTTCATGGTAGACCTCTTCTGc                                                                 >  2:938176/1‑289 (MQ=255)
                                                                                                                                                                                                                           cacCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACATAACGAATAGTAAt                                                                                                                     >  2:798111/1‑237 (MQ=255)
                                                                                                                                                                                                                           cacCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACATAACGAATAGTAAt                                                                                                                     <  1:798111/237‑1 (MQ=255)
                                                                                                                                                                                                                           cacCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACATAACGAATAGTAATCGTACTCCAGCTGCTGAGACTGAAGCTCAATTTCATGGTAGACCTCTTCTGc                                                                 <  1:247526/289‑1 (MQ=255)
                                                                                                                                                                                                                                cggcgTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACATAACGAATAGTaa                                                                                                                      <  1:20016/231‑1 (MQ=255)
                                                                                                                                                                                                                                cggcgTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACATAACGAATAGTaa                                                                                                                      >  2:20016/1‑231 (MQ=255)
                                                                                                                                                                                                                                cggcgTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCATGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACATAACGAATAGTAATCGTACTCAAGCTGCTGAGCCTGAAGCTCAATTTCATGGTAAACTTCTTATGCGGtc                                                             >  1:543553/1‑288 (MQ=255)
                                                                                                                                                                                                                                       agcctattgcactcgcgGCTACCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACATAACGAATAGTAATCGTACTCCAGCTGCTGAGCCTGAAGCTCAATTTCATGGTAGACCTCTTCTGCGGTCTCCATCct                                                     <  1:244819/276‑1 (MQ=255)
                                                                                                                                                                                                                                        aCCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACATAACGAATAGTAATCGTACTCCAGCTGCTGAGCCTGAAGCTCAAttt                                                                                    >  2:726654/1‑257 (MQ=255)
                                                                                                                                                                                                                                        aCCATGGGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACATAACGAATAGTAATCGTACTCCAGCTGCTGAGCCTGAAGCTCAAttt                                                                                    <  1:726654/257‑1 (MQ=255)
                                                                                                                                                                                                                                            tgtgCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACATAACGAATAGTAATCGTACTCCAGCTGCTGAGCCTGAAGCTCAATTTCATGGTAGACCTCTTCTg                                                                  <  1:422075/271‑1 (MQ=255)
                                                                                                                                                                                                                                            tgtgCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACATAACGAATAGTAATCGTACTCCAGCTGCTGAGCCTGAAGCTCAATTTCATGGTAGACCTCTTCTg                                                                  >  2:422075/1‑271 (MQ=255)
                                                                                                                                                                                                                                             gtgCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCTCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACATAACGAATAGTAATCGTACTCCAGCTGCTGAGCCTGAAGCTCAATTTCATGGTAGACCTCTTCTGCGGTCTCCATCCTCTGaaaa                                              <  2:241692/289‑1 (MQ=255)
                                                                                                                                                                                                                                             gtgCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCTCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACATAACGAATAGTAATCGTACTCCAGCTGCTGAGCCTGAAGCTCAATTTCATGGTAGACCTCTTCTGCGGTCTCCATCCTCTGaaaa                                              >  1:241692/1‑289 (MQ=255)
                                                                                                                                                                                                                                             gtgCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTAATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGATCAGCACCGGATCAATTGCGAGAACGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAACAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACCTCACGAATAGTAATACTACTCCCGCTGCTGAGACTCAAGCCCCATTTCATGGTAGACCTCTTCTGCGGTCGCCATCCTCTGaac                                               >  1:909242/1‑288 (MQ=255)
                                                                                                                                                                                                                                                   gcgcgGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACATAACGAATAGTAATCGTACTCCAGCTGCTGAGCCTGAAGCTCAATTTCATGGTAGACCTCTTCTGCGGTCTCCATCCTCTGAAAACGCaa                                         <  2:928333/289‑1 (MQ=255)
                                                                                                                                                                                                                                                          ttCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACATAACGAATAGTAATCGTACTCCAGCTGCTGAGCCTGAAGCTCAATTTCATGGTAGACCTCTTCTGCGGTCTCCATCCTCTGAAAACGCAACAGCATc                                  >  1:473960/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                       tgtgCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACATAACGAATAGTAATCGTACTCCAGCTGCTGAGCCTGAAGCTCAATTTCATGGTAGACCTCTTCTg                                                                  <  2:671372/244‑1 (MQ=255)
                                                                                                                                                                                                                                                                       tgtgCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACATAACGAATAGTAATCGTACTCCAGCTGCTGAGCCTGAAGCTCAATTTCATGGTAGACCTCTTCTg                                                                  >  1:671372/1‑244 (MQ=255)
                                                                                                                                                                                                                                                                       tgtgCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACATAACGAATAGTAATCGTACTCCAGCTGCTGAGCCTGAAGCTCAATTTCATGGTAGACCTCTTCTGCGGTCTCCATCCTCTGaa                                                >  2:33757/1‑262 (MQ=255)
                                                                                                                                                                                                                                                                       tgtgCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACATAACGAATAGTAATCGTACTCCAGCTGCTGAGCCTGAAGCTCAATTTCATGGTAGACCTCTTCTGCGGTCTCCATCCTCTGaa                                                <  1:33757/262‑1 (MQ=255)
                                                                                                                                                                                                                                                                       tgtgCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACATAACGAATAGTAATCGTACTCCAGCTGCTGAGCCTGAAGCTCAATTTCATGGTAGACCTCTTCTGCGGTCTCCATCCTCTGAAAACGCAACAGCATCGTGCGACGCCAg                      <  2:523765/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                        gtgCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACATAACGAATAGTAATCGTACTCCAGCTGCTGAGCCTGAAGCTCAATTTCATGGTAGACCTCTTCTGCGGTCTCCATCCTCTGAAAACGCAACAGCATCGTGCGACGCCa                       >  1:43387/1‑286 (MQ=255)
                                                                                                                                                                                                                                                                         tgCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACATAACGAATAGTAATCGTACTCCAGCTGCTGAGCCTGAAGCTCAATTTCATGGTAGACCTCTTCTGCGGTCTCCCTCCTCTGAAAACGCAACAGCATCGTGCGACGCCAGCTg                   >  2:404893/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                  gAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACATAACGAATAGTAATCGTACTCCAGCTGCTGAGCCTGAAGCTCAATTTCATGGTAGACCTCTTCTGCGGTCTCCATCCTCTGAAAACGCAACAGCATCGTGCGACGCCAGCTGAAAAAATcc          <  1:355617/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                     tAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACAt                                                                                                                                  <  2:675290/166‑1 (MQ=255)
                                                                                                                                                                                                                                                                                     tAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACAt                                                                                                                                  >  1:675290/1‑166 (MQ=255)
                                                                                                                                                                                                                                                                                       aGTCATCATTACACATTAAATGGCCCTGACTAAACTTTGCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTGTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCTGACACATAACGAATAGTAATCGTACTCCAGCTGCTGAGCCTGAAGCTCAATTTCATGGTAGACCTCTTCTGCGGTCTCCATCCTCTGAAAACTCAACAGCATCGTGCGACGCCAGCTGAAAAAATCCTTa       <  2:909242/287‑1 (MQ=255)
                                                                                                                                                                                                                                                                                        gTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCAACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACATAACGAATAGTAATCGTACTCAAGCAGCTGAGCCTGAAGCTCAATTTCATGGTAGACCTCTTCTGCGGTCCACATCCTCTGAAAACGCAACAGCATCGTTCGACGCCAGCTGAAAAAATCCtaaa      >  1:214798/1‑284 (MQ=255)
                                                                                                                                                                                                                                                                                          catcatTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACATAACGAATAGTAATCGTACTCCAGCTGCTGAGCCTGAAGCTCAATTTCATGGTAGACCTCTTCTGCGGTCTCCATCCTCTGAAAACGCAACAGCATCGTGCGACGCCAGCTGAAAAAATCCTTATCCTg  <  1:685914/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                                                               
CCGTCCACCTCAGAATTTCTTTTTCGCGCTTGCTGAAATTCATCTCTGGCGTCATCACTATTTCATCATTTAAACGCATCAGAGCCATCAGACTTTCGCGCACCAGTAACTGCATTTTTAATTGCAGTTCATCACTAAGAATGGGTATTTCGCGCGCGCTGCAACGGGAAAAGGACAAAAAGCCCAGCGCCCGGTTTGGCAGCATTAAATACTGAGTGACACCGCGGCGTAAACCATGTGCGCGCGCGGCTTCCCATAACGGCTGTGCTTCGCTGAATAAGTCATCATTCCACATTAAATGGCCCTGACTAAAGTTTTCAGGGTTCAGCACCGGATCAATTGCGAGAAAGTTTTTTGCCTGATAATAACTAACCCACGCCTCAGGGTAATTGGTGTAAAAAGCCACTTTAGGTCGAGTGAATGGTACCGGGTGGCGGACACATAACGAATAGTAATCGTACTCCAGCTGCTGAGCCTGAAGCTCAATTTCATGGTAGACCTCTTCTGCGGTCTCCATCCTCTGAAAACGCAACAGCATCGTGCGACGCCAGCTGAAAAAATCCTTATCCTG  >  NC_000913/1996259‑1996829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: