Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	75,586	A→T	4.8%	intergenic (‑106/+58)	thiB ← / ← sgrR	thiamine/thiamine pyrophosphate/thiamine monophosphate ABC transporter periplasmic binding protein/transcriptional DNA‑binding transcriptional activator of sgrS sRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	75,586	0	A	T	4.8%	352.0 / 5.5	97	intergenic (‑106/+58)	thiB/sgrR	thiamine/thiamine pyrophosphate/thiamine monophosphate ABC transporter periplasmic binding protein/transcriptional DNA‑binding transcriptional activator of sgrS sRNA
Reads supporting (aligned to +/- strand):  ref base A (43/49);  new base T (3/2);  total (46/51)
Fisher's exact test for biased strand distribution p-value = 6.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

CCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGATTCATCTCGCCATTGCGCCAGCGAGCAGCGTCGGCTTGCCAGTCAATGGGAATGCAAT  >  NC_000913/75309‑75864                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                         ccAGCGCCACCAGTTTCAGTTCGCAATTACAGTAGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCAcc                                                                                                                                                                                                                                                                               <  1:919593/289‑1 (MQ=255)   aGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCACATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCAcccc                                                                                                                                                                                                                                                                             >  1:719788/1‑289 (MQ=255)   aGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCAcccc                                                                                                                                                                                                                                                                             >  2:536683/1‑289 (MQ=255)    gcgcCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCg                                                                                                                                                                                                                                                                            >  2:916609/1‑289 (MQ=255)         accaGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGCGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTAAGCGCACGCGTACGCGGGGCCCACCG‑TTgag                                                                                                                                                                                                                                                                      >  1:203342/1‑289 (MQ=255)           caGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGaa                                                                                                                                                                                                                                                                    <  2:198117/289‑1 (MQ=255)            acgttCCGTTCGCAATTACAATCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGGCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTGACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCGAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAAc                                                                                                                                                                                                                                                                   <  1:633020/286‑1 (MQ=255)                 cgattCGCAATCACAGTCCGCATCAAAGGCTTTTTAAACCACCTGACACGGCCCCCAGCCGGGGGCGAAGGAATCGTAGGTCTAAACAGTCAGATCGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACCGCAGGGGCAGACATTGTTTTAACCCTTTGCACCTCAAAAAAGGGTGGCAAAGGACTTGAGAAGGGGCCTCCAATCCCTTCG‑CCGGCGTTATCCGGATCGGGTTCTACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGt                                                                                                                                                                                                                                                              <  1:298414/286‑1 (MQ=255)                         aaTTACAGTCGGCTTCAAAGGCTTTGTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAAAGGCGTtagtgta                                                                                                                                                                                                                                                       <  2:740208/288‑1 (MQ=255)                                 tCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTTCGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTg                                                                                                                                                                                                                                              >  1:499504/1‑289 (MQ=255)                                   ggCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACCGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTTTCCGGATCAGGTTCGACGGGCATTTTCTCAGCGAACGCGTACGCGTGGCACCCCG‑TTGAGAACGGAGTTAGTGTAGTGATTTTg                                                                                                                                                                                                                                              >  2:114217/1‑287 (MQ=255)                                              cTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAg                                                                                                                                                                                                                                   <  2:719788/287‑1 (MQ=255)                                              cTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGc                                                                                                                                                                                                                                  <  1:264497/288‑1 (MQ=255)                                              cTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGGTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGGGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAg                                                                                                                                                                                                                                   <  1:12101/287‑1 (MQ=255)                                               ttttttAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTTCGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCaa                                                                                                                                                                                                                                >  1:469991/1‑289 (MQ=255)                                               ttttttAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGaa                                                                                                                                                                                                                                                                    >  1:865109/1‑253 (MQ=255)                                               ttttttAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCa                                                                                                                                                                                                                                 <  2:706363/288‑1 (MQ=255)                                               ttttttAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCGCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGaa                                                                                                                                                                                                                                                                    <  2:865109/253‑1 (MQ=255)                                                      accaccGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGa                                                                                                                                                                                                                         <  2:624048/289‑1 (MQ=255)                                                           cGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGCACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCCGCAATACTGGATCCgg                                                                                                                                                                                                                    >  1:651800/1‑289 (MQ=255)                                                                       ccAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAACCATAGATACGGTGGCG‑TCAAccc                                                                                                                                                                                                       >  1:648325/1‑288 (MQ=255)                                                                           tCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGc                                                                                                                                                                                                    <  2:599021/288‑1 (MQ=255)                                                                            cggcggGGAAAGAATCTTAGGTATAAACAGGCAGAACGGATTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGc                                                                                                                                                                                                    <  2:900954/287‑1 (MQ=255)                                                                            cggcggCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAACACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGc                                                                                                                                                                                                    <  2:648325/287‑1 (MQ=255)                                                                            cggaggCGAAGGAATCGGAGGTGTAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGAAACAGCAGGGGCAGACGTTTTTGTAACACTTTGCACCTCAAAAAAGAGTTGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGc                                                                                                                                                                                                    <  1:656869/287‑1 (MQ=255)                                                                               cggcgAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGAtt                                                                                                                                                                                               >  1:646454/1‑289 (MQ=255)                                                                                          tCGTAGGTATAAACAGTCCGAACGGGTTTAGCGAAAACGGACGCTGGGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTTCACCTCAAAAAAGAGTGGCAAAGGACGTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTGGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAAc                                                                                                                                                                                    <  1:187810/289‑1 (MQ=255)                                                                                                 tataAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGcc                                                                                                                                                                               <  2:603677/287‑1 (MQ=255)                                                                                                 tataAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGGGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGcc                                                                                                                                                                               <  2:972238/287‑1 (MQ=255)                                                                                                  ataAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGa                                                                                                                                                                             <  1:349790/288‑1 (MQ=255)                                                                                                  ataAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTCTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGa                                                                                                                                                                             <  2:745059/288‑1 (MQ=255)                                                                                                   taAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATACGGTGGCT‑CAAACCACGCTGATTTAACATCGCACCAGCCgggg                                                                                                                                                                           >  1:104160/1‑289 (MQ=255)                                                                                                        aGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTAtt                                                                                                                                                                      <  1:916609/289‑1 (MQ=255)                                                                                                          tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTCTTCa                                                                                                                                                                    >  1:229134/1‑289 (MQ=255)                                                                                                          tCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCa                                                                                                                                                                    >  2:426076/1‑289 (MQ=255)                                                                                                           cAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCAt                                                                                                                                                                   >  2:746675/1‑289 (MQ=255)                                                                                                            aGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATg                                                                                                                                                                  >  2:784182/1‑289 (MQ=255)                                                                                                               aCGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATgcgc                                                                                                                                                               <  2:249212/289‑1 (MQ=255)                                                                                                                 gggTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCGTGCGCAg                                                                                                                                                             <  2:8619/289‑1 (MQ=255)                                                                                                                 gggTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAg                                                                                                                                                             <  2:646454/289‑1 (MQ=255)                                                                                                                 gggTTTAGCGAAAACGGGCGATGTGCACAGAAACAGAAAGGGCAGACAGTTTTTTAGCACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGAGGGGTATTTTCTCAGCGCACGGGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTGATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAg                                                                                                                                                             <  1:257797/289‑1 (MQ=255)                                                                                                                  ggTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑AAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAg                                                                                                                                                             <  2:706295/288‑1 (MQ=255)                                                                                                                    tttAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTTCGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGc                                                                                                                                                           <  2:499504/288‑1 (MQ=255)                                                                                                                    tttAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGc                                                                                                                                                           <  1:295721/288‑1 (MQ=255)                                                                                                                    tttAACGAAAACGGGCGCTGTGGACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCGTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGc                                                                                                                                                           <  1:114217/288‑1 (MQ=255)                                                                                                                             aaCGGGCGCTGTGCACAGCACCAGCCGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGGACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGTG‑CAAACCCCGCTGCTTTAAAACCGAACAACCCGAGGGTATTCCTGCGCAGGCCGCGCATAc                                                                                                                                                  >  1:570526/1‑288 (MQ=255)                                                                                                                             aaCGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCACCCAGCAATCATGTATCCGGTGGCC‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCAt                                                                                                                                                    >  1:767255/1‑286 (MQ=255)                                                                                                                                gggCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCg                                                                                                                                              <  2:691047/289‑1 (MQ=255)                                                                                                                                gggCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCg                                                                                                                                              <  2:300077/289‑1 (MQ=255)                                                                                                                                gggCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCg                                                                                                                                              <  2:229134/289‑1 (MQ=255)                                                                                                                                           acagcaacagcaGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGaa                                                                                                                                   <  1:342166/289‑1 (MQ=255)                                                                                                                                             agcaacagcaGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCCGGCCGCGCATACTGCGTTGCCCCTGAATg                                                                                                                                 >  2:623800/1‑289 (MQ=255)                                                                                                                                                aacagcaGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGa                                                                                                                                <  1:784182/287‑1 (MQ=255)                                                                                                                                                  cagcagGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCCGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGATGATTTAAAATCGAACCAGCCGAGGGTATTCAGGCCCAGGCAGCGAATACTGCGTTGCCCCTGacacaaca                                                                                                                             >  1:177337/1‑281 (MQ=255)                                                                                                                                                  cagcagGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTGGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTCGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCa                                                                                                                             >  2:473897/1‑288 (MQ=255)                                                                                                                                                  cagcagGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCCGCCGAGGGTATTCATGCGCAGGCCGCGCATCCTGCGTTGCCCCTGAATGATCa                                                                                                                             >  1:607100/1‑288 (MQ=255)                                                                                                                                                  cagcagGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCACTGAATGATCa                                                                                                                             >  2:854415/1‑288 (MQ=255)                                                                                                                                                  cagcagGGGCAGACATTTTTTTAACACTTTGCACCCCAAAAAAAAGTGCCAAA‑GACTTTCAAAGGCGCCCTAAAACCCCTCGCCCGCCGTAACCCGGACAGGGTTGGACGGGCATTTCCCCAGC‑CCCGCGTTCGCCGGGCACCCCGTTTCAGACGGCCGTTGGGGTAG‑GCTTTTTTTTACCACCCGCAAACCTGGAACCCGTGGCGCCAACCCAAGCTGTTTAAAAATCACACAAGCCAAGGGTATTCATGCCCACGCGCCGAATAAT‑CGTTTCCCCCGaaatgacca                                                                                                                            >  1:91479/1‑281 (MQ=255)                                                                                                                                                      agGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCCCGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGc                                                                                                                           <  2:242144/286‑1 (MQ=255)                                                                                                                                                       ggggCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAACATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGAGCATCCTGCTTTGCCCCTGACTGATCAGCCAgt                                                                                                                       >  1:486866/1‑289 (MQ=255)                                                                                                                                                         ggCAGACATTTTTTTAACACTTTGCACCTCAAGAAAGGGTGGCAAAGGACTTGAGAATGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCATCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAg                                                                                                                        <  2:483948/286‑1 (MQ=255)                                                                                                                                                         ggCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAg                                                                                                                        <  2:151086/286‑1 (MQ=255)                                                                                                                                                            aGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGc                                                                                                                  <  1:811940/289‑1 (MQ=255)                                                                                                                                                             gACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGCATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGACGGTATTCATGAGCGGGCCGCGCTTACTGCGTTTCCCATGACTTATCAGCTCTTGGTGcc                                                                                                                 >  1:489155/1‑288 (MQ=255)                                                                                                                                                                  ttttttAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTTCGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCaataat                                                                                                            <  2:469991/289‑1 (MQ=255)                                                                                                                                                                          cacTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTta                                                                                                                                                                                                                                                            >  2:122712/1‑138 (MQ=255)                                                                                                                                                                          cacTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTta                                                                                                                                                                                                                                                            <  1:122712/138‑1 (MQ=255)                                                                                                                                                                              ttGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCt                                                                                                >  2:610627/1‑289 (MQ=255)                                                                                                                                                                              ttGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCt                                                                                                >  2:605277/1‑289 (MQ=255)                                                                                                                                                                               tGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCg                                                                                                                                                                                                                                                               >  1:178060/1‑130 (MQ=255)                                                                                                                                                                               tGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCg                                                                                                                                                                                                                                                               <  2:178060/130‑1 (MQ=255)                                                                                                                                                                               tGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATCATGGCACCATCGCtt                                                                                               >  2:166620/1‑289 (MQ=255)                                                                                                                                                                                         aaaaGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGAc                                                                                     <  1:426076/289‑1 (MQ=255)                                                                                                                                                                                              agTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCCCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATGTTGTTATCCACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAAGCGAACCAGCCGAGGGTATTAAGGCGCACGCCGCGCACACTGCGTTTCCCCTGAATGATCAGCCAGTGGTGAAATAATGGCAACATAGCTTTGTTGGCGACCAGtt                                                                                >  1:36275/1‑289 (MQ=255)                                                                                                                                                                                                        gACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCACG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGCTGCTGGCACCa                                                                      >  2:704962/1‑289 (MQ=255)                                                                                                                                                                                                                  ggAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCCCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGCGTAGTGATTTTGTTATCAACCAGCAATCATGGAACCGGTGGCG‑CAATCCAGGCTGAATTTAAATCGAACCAGCCGACGGTATTCATGCGCAGGCCGCGCATACCGCGTTCCCCCTATACGCTCAGCCAGTCGTCCCATAATGGCACCAACCCTTTGCTTGCGCCCacctcccgtactttgctcgccag                                                             >  1:436329/1‑266 (MQ=255)                                                                                                                                                                                                                   gAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGAt                                                           >  2:644856/1‑289 (MQ=255)                                                                                                                                                                                                                     gCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGCTTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGGCGCGCATACTGCGTTGACCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCAGCGGTTTGCTGGCGACCAGTTGCTGGCACCAGCTCGCCAGATTc                                                         >  2:819974/1‑289 (MQ=255)                                                                                                                                                                                                                        tCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGATTCa                                                        >  2:685279/1‑287 (MQ=255)                                                                                                                                                                                                                         cAATTCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGATTCAtct                                                     <  1:303231/289‑1 (MQ=255)                                                                                                                                                                                                                         cAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGATTCAtc                                                      >  2:917164/1‑288 (MQ=255)                                                                                                                                                                                                                              cccTTCG‑CCGGCGGTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGATTCATCTCGCCa                                                <  1:536683/289‑1 (MQ=255)                                                                                                                                                                                                                               ccTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGAGTAGTGCTTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGACGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATTGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGATTCATCTCGACAt                                               >  2:126168/1‑289 (MQ=255)                                                                                                                                                                                                                                 ttCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTCGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAGTGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGATTCATCTCGCCAtt                                              >  2:366738/1‑288 (MQ=255)                                                                                                                                                                                                                                  tCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCt                                                                             <  1:260829/256‑1 (MQ=255)                                                                                                                                                                                                                                  tCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCt                                                                             >  2:260829/1‑256 (MQ=255)                                                                                                                                                                                                                                          cGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAAGAATGGCACCATCGCTTTTCTGGCGACCAGTTACTGGCACCAGCTCGCCAGATTCATCGCGCCATTGAGCCTGCg                                     >  2:876840/1‑289 (MQ=255)                                                                                                                                                                                                                                             tATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGATTCATCTCGCCATTGCGCCAGCGag                                   <  1:605277/288‑1 (MQ=255)                                                                                                                                                                                                                                                  ggATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGATAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCAGGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGATTCATCTCGCCATTGCGCCAGCGAGCAGCGt                             <  2:971972/289‑1 (MQ=255)                                                                                                                                                                                                                                                    aTCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCTTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGATTCATCTCGCCATTGCGCCAGCGAGCAGCGTCg                           >  2:70508/1‑289 (MQ=255)                                                                                                                                                                                                                                                          ttCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGATTCATCTCGCCATTGCGCCAGCGAGCAGCGTCGGCTTGc                     <  2:651800/289‑1 (MQ=255)                                                                                                                                                                                                                                                              aCGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGATTCATCTCGCCATTGCGCCAGCGAGCAGCGTCGGCTTGCCAg                  >  2:242390/1‑288 (MQ=255)                                                                                                                                                                                                                                                                   tATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGATTCATCTCGCCATTGCGCCAGCGAGCAGCGTCGGCTTGCCAGTCAATg            >  2:90238/1‑289 (MQ=255)                                                                                                                                                                                                                                                                    aTTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTCGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAGTGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGATTCATCTCGCCATTGCGCCAGCGAGCAGCGTCGGCTTGCCAGTCAATgg           <  1:366738/289‑1 (MQ=255)                                                                                                                                                                                                                                                                      tttgtaaGCGTACGCGTAAGAGTGTGACCCCG‑TTGGGAACTGCGTTAGTGTAGTGATTTTTTTCGCAAGCAGCAATTATGGATCCGGTGGCT‑CAAAGCACGCTGATTAAGAATCGAACAAGCCGAGGGGATTCATTCGCAGGCAGCGCATACTGAGTTGCGCCTGAATGATCAGCCAGTGGTGCAATACTGGCACCATCGCTTTGCTGGCGACCAATTGCTGGCACCGGTTCGCCAGATTCATCTCGCCATTGCGCCAGCGAGCAGCGTCGGCTTGCCAGTCAAtttga         <  1:296910/283‑5 (MQ=255)                                                                                                                                                                                                                                                                           cAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGATTCATCTCGCCATTGCGCCAGCGAGCAGCGTCGGCTTGCCAGTCAATGGGAATGCa    <  2:767284/289‑1 (MQ=255)                                                                                                                                                                                                                                                                             gcgcACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGATTCATCTCGCCATTGCGCCAGCGAACAGCGTCGGCTTGCCCGTCGATGGGaacgacat  >  1:882032/1‑283 (MQ=255)                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                         CCAGCGCCACCAGTTTCAGTTCGCAATTACAGTCGGCTTCAAAGGCTTTTTTAACCACCGGACCAGGCCCCCAGTCGGCGGCGAAGGAATCGTAGGTATAAACAGTCAGAACGGGTTTAGCGAAAACGGGCGCTGTGCACAGCAACAGCAGGGGCAGACATTTTTTTAACACTTTGCACCTCAAAAAAGAGTGGCAAAGGACTTGAGAAGGAGCCTCAAATCCCTTCG‑CCGGCGTTATCCGGATCAGGTTCGACGGGTATTTTCTCAGCGCACGCGTACGCGTGGCACCCCG‑TTGAGAACGGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCG‑CAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAGTGGTGCAATAATGGCACCATCGCTTTGCTGGCGACCAGTTGCTGGCACCAGTTCGCCAGATTCATCTCGCCATTGCGCCAGCGAGCAGCGTCGGCTTGCCAGTCAATGGGAATGCAAT  >  NC_000913/75309‑75864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑