Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,708,844 C→G 9.1% A642A (GCC→GCG rsxC → SoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,708,8440CG 9.1% 145.7 / 2.6 62A642A (GCC→GCGrsxCSoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex
Reads supporting (aligned to +/- strand):  ref base C (36/18);  new base G (2/5);  total (38/24)
Fisher's exact test for biased strand distribution p-value = 9.25e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTA  >  NC_000913/1708565‑1709129
                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                              
tgagatagacgagagaCGTCAAACGGCCAGTGAACCACATACAGCCTGCGCCAAAGCGCGCAAAGTGTAACAGCAAAAGCGTAGTGAGGAGCCGAAGCAACTGAGCGATCCGCGCAAAGCCGCCGTCGAAGCTGCTATTGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAg                                                                                                                                                                                                                                                                                         <  2:374527/272‑1 (MQ=14)
            tCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGACCAGCAAAAGGCTAATGACGAGCCCGAAAAACAGGTCGa                                                                                                                                                                                                                                                                            >  1:703937/1‑288 (MQ=255)
                          ccGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTAGTGCCCGTGCCAAAGCCCGCAAGCTGGAACAGCAACAAGCTAATGCCGAGCAAGAACAACAGGTcccgtctcctatcaaca                                                                                                                                                                                                                                                              >  1:374527/1‑272 (MQ=255)
                          ccGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTAGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGAAACCTGGAACAGCAACAGGCTAATGCCGAGCCAGCACAACAGGTCGATCTGCGCAAAgccg                                                                                                                                                                                                                                                              >  1:638703/1‑288 (MQ=255)
                          ccGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATACGGAACCAGAAGAACAGGTCGATACGCGCAAAGCCGCCGTCGCAGCCGCTATTGCCAGTGCCAAAGCACGCAAGCCGGAAAAGCAAAAGGCTAATGACGAGCCAGAACTACAAGTAAATCCTCGAAAAgccg                                                                                                                                                                                                                                                              >  1:458997/1‑288 (MQ=17)
                                 agcagcTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCGACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGa                                                                                                                                                                                                                                                       <  1:59898/288‑1 (MQ=255)
                                      cTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaaca                                                                                                                                                                                                                                                                                     <  1:708976/253‑1 (MQ=255)
                                                cgcCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATACGGAACCAGAAGAACAGGTCGAACCGCGCAAAGCCGACGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGACTAATGCCAAACCAGAACAACAGATAGAGCCGCGCAAAGCAGCCGTTTAAGCAGCAATTGCCCGa                                                                                                                                                                                                                                      >  1:803814/1‑289 (MQ=14)
                                                                           aCAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGACCGAGCCAAAGCGCCCAAACGGGACCAGCaa                                                                                                                                                                                                           >  1:355065/1‑289 (MQ=255)
                                                                           aCAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAACCAGAAGAACAGGTCGATCCGCTCCAAGCCGCCAACGAAGCCGCTATTGCCCGAGCCAAACCGCGCAAACGGCAACAGCa                                                                                                                                                                                                            >  1:441993/1‑288 (MQ=255)
                                                                             aGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGGGCCAAAGCACGCAAACGGGAACAGCAAcc                                                                                                                                                                                                         >  1:676802/1‑289 (MQ=255)
                                                                                       ggACCCAGAACCACAGGTCGATCCGCGCACACCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGAGCAAGCTGGAACAGCAACAGCCTAATGCGGACCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCg                                                                                                                                                                                               <  1:367377/289‑1 (MQ=255)
                                                                                         aaCCAGAACAACAGTTCGATCCTCTCAAAGCCGCCGTCGAAGCCGCAATTGCCCGTGCCAAAGCTCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGt                                                                                                                                                                                                                                                          <  1:368601/229‑1 (MQ=255)
                                                                                         aaCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGCGCCCGAACAACAGGTCGCTCCGCGCAAAGCCGCCGTCGAAGCCGCTATTCCCCGAGCCAAAGCCCGCAAACGGGAACAGCAACCGCCTAACGCGGa                                                                                                                                                                                             >  1:313653/1‑289 (MQ=255)
                                                                                         aaCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGATATACACCGTGCCAAAGCACGCAAGCGGGAACAGCAACAGGCTAATGCCGAGCAAGCACAACAGGTAGAACCGCCCAAACCCGCCGCCGACGACGCTATAGCCCGCACCCAAAAGCCGAAAACGGAACAGCAACAGGCGAATGGGGa                                                                                                                                                                                             >  2:649171/1‑289 (MQ=14)
                                                                                                  aacaGGGCGAGCCGCGCAAAGCTGCCGTCGAAGCAGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCagaaga                                                                                                                                                                                    <  1:581297/289‑1 (MQ=255)
                                                                                                  aacaGGCCGATCCGCGTAAAGCCGCCGTCGAAGCCGCGATTGCCCGTGCCAAAGCGGGCAAGCGGGAACAGCAACAGGCTAATGCGGAACCCGAAGAACAGGTCGCTCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCagaaga                                                                                                                                                                                    <  1:743784/289‑1 (MQ=255)
                                                                                                           aTCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCCCGCAAGCTGGAACAGCAACAGGCTAATGCCGTGCCAGAAAAACAGGTCGATCCCCGCAAAGCCGCAGTCGACGCCGCAATTGCCCGGCCCAAGGCCCACAAACCAGAACAGCAACAGGCTAATgacgagtcacaaaaaaagctcta                                                                                                                                                                           >  2:519440/1‑267 (MQ=14)
                                                                                                            tCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAACCCGAATAACAGGTCGATCCTCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTCCCAAAGCACGCAAACTGGAACAGCAACAGGCTAATGCCTATCCCGCACACCCAGTCGAACCCCCCGAAGCCACCGTTGAAGCCACCATTCCCGCCGCCAAAACCACTCAACGAGCACAGCACACAGATAATGTGCAGCCagaaaccaaagccac                                                                                                                                                                           >  2:51961/1‑277 (MQ=2)
                                                                                                                 gcAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGCACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCCAAGCCCGCCAGCTGGAACAGCCACAGGCTATTGCCGCACCATAACAACAGGTTGATCCACCTAAACCCGCCGTCACCACCGCCATTGCCCCAGCCACTGCCCGCCAACAGCCCTTGCTACCCGCTCACGCGGACGCAGCAGAACAGGtacactcgc                                                                                                                                                                      >  2:733134/1‑280 (MQ=9)
                                                                                                                  cagaGCCGCAGGCGAAGCCGCTATTGCCCGTGCGAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCcgcg                                                                                                                                                                     <  1:649171/285‑1 (MQ=255)
                                                                                                                              tgaagcgcgtATCGCCCGCGCCAAAGCGGGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCCGAACACCAGGTCAATCCGCCCAAAACCGCCGTCGAAGCCGCTATTACCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGt                                                                                                                                                         <  1:276116/279‑1 (MQ=14)
                                                                                                                                 aGCCGCTAGTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGa                                                                                                                                                      <  1:287411/288‑1 (MQ=255)
                                                                                                                                   acGCCATCGCCCGCGACAAAACACGCAATCGTGATAATCAACAGGCTAAGGCTGAAACAGAGAGAAAAGGCGACAGGCGCAAAGCAGCCGTCGATGCTGCTAGTGCCCGCGGCAAAGCACGCAAGCTAGAACAGCAACAGGCTAATGCGTGGCAAGAACGACTAGTCGATCCGCGAAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGc                                                                                                                                                   <  2:638703/288‑1 (MQ=255)
                                                                                                                                                           gcAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCCGAACAACAGGTCGAACAGCGCAAAGCCGCCGTCGAAGCGGCTATTGCCCGCGCAAAAGGCCGCAAACGGGAACAGCAACCCGCTAATGAGGAGCCAGAAGAACAGGTTGAGCCGCGCAAAGCCGCCGTCGACACGGCTATTCACCGCGCCAAGGCaa                                                                                                                            >  2:865525/1‑287 (MQ=14)
                                                                                                                                                             aaGCGGGAACAGCAACAGGCTAATGCGGAACCAGAACAACCGGTCGACCCGGGCAAAGCCGCCGTCGAAGCCGCTATTACCCGGGCCAAAGCACGCAAGCAGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCa                                                                                                                         <  1:382549/289‑1 (MQ=255)
                                                                                                                                                                        gcgaCAGGCAACTACGGCAGCAGAAACACTGGTCGAGCCGCGTAAAACCGCCGCCGAAGCCGCTATGACACGGGCCAAAGCACCGAAGCTGGAACAGCAACAGGCGAATGCGGAGGCAGAACAACATGTCGATCCGGGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAGCTGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAAc                                                                                                                <  2:824531/284‑1 (MQ=255)
                                                                                                                                                                         cAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAAAAACAGGTCGATCCGCCCAAAGCCGCCGACGAAGCCGCTATTTCCCGAGCCAAAGCGCGCAAACCGGACCAGCAACCGGCTAATGCGGAGCCAGAAAAGAGGGTTGAATAGAGCAAAGCCGCCGTCGAACCGGCTATTGGCCGCGCCAAAggaacaaagcagggcgcag                                                                                                             >  2:635251/1‑271 (MQ=14)
                                                                                                                                                                           acgggCTAATGCGGAACAAGAAGAACAGGTCGATCCACGCAAAGCCGCCGTTGAAGCCGCTATTGCCCGTGCCAAAGCACGCAGGGTGGAACAGCAACAGGCTAAAGCGGAGCAAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAGCGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCaa                                                                                                           <  1:51961/286‑1 (MQ=17)
                                                                                                                                                                                cTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCAGCTATTGCCCGTGACAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACa                                                                                                         <  1:698573/286‑1 (MQ=255)
                                                                                                                                                                                         aaCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACCGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGCAGCGGCTATTGCACGCGCCAAAGCACGCAAGCCGGAACAGCACCAGCCTAATgagc                                                                                              >  1:104018/1‑285 (MQ=255)
                                                                                                                                                                                         aaCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGATCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCTGTCCAAGCGGCTATTGCCCTCGCCAACGCACGCCAGCTGGAACAGCACAAGGCCAATGCGGt                                                                                             >  1:790061/1‑289 (MQ=17)
                                                                                                                                                                                         aaCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCCCGCAAACCGGAACAGCAACAGCGCAATGCGGAGACAGAAGAACAGGTTGATCAGCGCAAAGCTGCCGTGTCAGCGGCTATTGCCCGGGCCTACGCCCGCCACCAGAAACAGCACCAGGTCAATGCggg                                                                                             >  2:839615/1‑288 (MQ=14)
                                                                                                                                                                                         aaCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCACGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCACAAAGCCGCCGTCGAAGCGGCAATTGCACGAGCAAAAGCCAGCAAAGCGGAACACCAAACGGCTAATGCGGt                                                                                             >  2:386898/1‑289 (MQ=14)
                                                                                                                                                                                               aagaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCCGGAACAGCAACAGGCTAATGCGGTACCaga                                                                                       >  1:551170/1‑289 (MQ=255)
                                                                                                                                                                                                 aaaaCAGGTCGACCCGCGCAAGGCCGCCGTCGAAGCCGCTATTGCACGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCag                                                                                        <  1:386898/285‑1 (MQ=255)
                                                                                                                                                                                                    tctggttgttCCGCTCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCCGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATCGCCCGAGCCAAAGCGCGCAAGCTGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCagaaga                                                                                    <  1:297003/279‑1 (MQ=255)
                                                                                                                                                                                                      aGGACGATCCGGGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCAGCCGTCGAAGTCGATATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCTGCCAAGGCGGAGCCAGACGAAAAGGATGATGCGCGCAATGCGGCCGTCAACGCTGCCCTTGCACGCGCCAACGGCAGCAAGCCGGAACAGCAACAGGCTCATGCGGTACCACACGAAcc                                                                                 >  2:709660/1‑288 (MQ=17)
                                                                                                                                                                                                        gTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCCCGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGCAGAACAgg                                                                               >  1:200845/1‑289 (MQ=255)
                                                                                                                                                                                                          cGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaa                                                                                                                                                                                                                                                                                       >  2:512738/1‑88 (MQ=255)
                                                                                                                                                                                                          cGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGaa                                                                                                                                                                                                                                                                                       <  1:512738/88‑1 (MQ=255)
                                                                                                                                                                                                             tCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGCACAGCAACAGGCTAATGCCGAGCCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGCACAGCAACCGGCTAATGCGGAGCCAGAAGCACAGTTTGATCCGCGCAAAGCTGCCGGTGCAGCGGCTCTGGCCCGAGACCAAGCCCGCAAGCTGGACCAGCAAACGGCTAAAGCggcagccacagaaaaggttagc                                                                          >  2:625841/1‑269 (MQ=14)
                                                                                                                                                                                                                 cgtaaaactgccgTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCTCCAAAACACGCCAGCTGGAACAGCCACAGGCTAAAGCGGAACCAGAAGAACAGGTCGAACCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGCGCCAAAGCACGCAAGACGGAACAGCAAAAGAGCAAAGCGGAACCAAAAACACAggccagacca                                                                       >  2:287411/10‑280 (MQ=14)
                                                                                                                                                                                                                 cgcAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAAAAGGCTAATGCGGTAGCAGAAGAACAGGTTGATCcg                                                                       >  1:644361/1‑288 (MQ=255)
                                                                                                                                                                                                                   cAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGaa                                                                                                                 >  1:688868/1‑244 (MQ=255)
                                                                                                                                                                                                                   cAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCAGCCGTAGAAGCGGCTCTTGCCCGCGCCAAAGCACGCAAACTGGAACAGCCACAGGTTAATGCGGTACCAAAAGAACAGGTAGAtacacgg                                                                    >  2:384343/1‑283 (MQ=17)
                                                                                                                                                                                                                                               cGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGccc                                        <  1:839615/289‑1 (MQ=255)
                                                                                                                                                                                                                                                 tGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAg                                                                                                                                                    >  2:32769/1‑179 (MQ=255)
                                                                                                                                                                                                                                                 tGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAg                                                                                                                                                    <  1:32769/179‑1 (MQ=255)
                                                                                                                                                                                                                                                     aacacACGCAAGCAGCAAAAACACAAGGTGAATGAGGAGGCAGAACGACAGTTCGAACCGCGAAAAGCCGCCGGGGAAGACGACGTTGCCGGGGCCAAAACGCGGAAACGGGAAGAACAACGGGCAAATGCGGAGCCAGGAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCt                                   <  2:371309/284‑1 (MQ=17)
                                                                                                                                                                                                                                                       aGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAg                                >  1:225115/1‑289 (MQ=255)
                                                                                                                                                                                                                                                           cGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGGTGCCGCGGCCATTGCCCGCGCTCAGGCCa                            >  1:839104/1‑289 (MQ=255)
                                                                                                                                                                                                                                                            gCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCCGAAGAAAAGGATGATCCGCGCCAAGCGGCCGTCGAAGCGGCTATTGCCCGCGCCAAAGCACGAAAACCGGAACGGAAACAGGCTAATGCGGTACCAGAAGAAACGGTTTGTCCGCGTAAACAGGCAGATgaccggtacatttccggcggtgcggcacc                           >  2:693655/1‑261 (MQ=14)
                                                                                                                                                                                                                                                            gCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGGGCTCAGGCCaa                           >  1:21080/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                          cAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGACCGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGAACGCGCCAAAGCACGCAAGCCGGAACAGCCACAGGCTAATGCGGTACCAGAAGAACAGGTTGAACCGCGCCAAGCGGCCGTTTCAGCGGCAATTTGCCGCGACCAGGCCcacaaacacccacagg             >  2:863600/1‑279 (MQ=17)
                                                                                                                                                                                                                                                                          cAACAGGCTAATGCCGCGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATACGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCCGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGCTCAGCCCAAAGCGGCAGTTGCCGCGGCTATTGCCAGAGCTCAGGCAAAAAAAGCCGccccg              >  1:848637/1‑286 (MQ=18)
                                                                                                                                                                                                                                                                          cAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGa                                                                                                                                                                                             <  1:475665/112‑1 (MQ=255)
                                                                                                                                                                                                                                                                          cAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGa                                                                                                                                                                                             >  2:475665/1‑112 (MQ=255)
                                                                                                                                                                                                                                                                                 cgaaggCCGAGCCAGACCAACATGTCTGTCCGCGCAAACCCGCCGTCGAAGCCTCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGTCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACGGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGaa         <  1:693655/281‑1 (MQ=255)
                                                                                                                                                                                                                                                                                 cTAATGCAGGGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACAGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGa          <  1:519440/285‑1 (MQ=255)
                                                                                                                                                                                                                                                                                    aTGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGAGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGCAGCCGCTATTGCACGCGCAAAAGCACGCAAGCGGGCACAGCCACAGGCTAATGCGGTACCAGAATAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTCCCCCCCCCACAGCCAAAAAAGCCTCCCAGCACAAgggcga   >  2:377780/1‑285 (MQ=18)
                                                                                                                                                                                                                                                                                     tGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTa  >  1:432004/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                              
AACCGTTGCTGATCCACGTAAAACTGCCGTTGAAGCAGCTATCGCCCGCGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCGCGCAAGCTGGAACAGCAACAGGCTAATGCGGAACCAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACGGGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCGGTACCAGAAGAACAGGTTGATCCGCGCAAAGCGGCAGTTGCCGCGGCTATTGCCCGCGCTCAGGCCAAAAAAGCCGCCCAGCAGAAGGTTGTA  >  NC_000913/1708565‑1709129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: