Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,269,859:1	+T	5.3%	noncoding (2/66 nt)	rdlB →	sRNA antisense regulator affects LdrB translation, proposed addiction module in LDR‑B repeat, with toxic peptide LdrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,269,858	1	.	T	5.3%	261.8 / 10.4	76	noncoding (1/66 nt)	rdlB	sRNA antisense regulator affects LdrB translation, proposed addiction module in LDR‑B repeat, with toxic peptide LdrB
Reads supporting (aligned to +/- strand):  ref base . (30/42);  new base T (2/2);  total (32/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

GAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAG  >  NC_000913/1269572‑1270146                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                   gAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAgtg                                                                                                                                                                                                                                                                                                     >  2:297021/1‑287 (MQ=35) gAGGCTTCCAGCTCGGGTTGCAAATATTTACTTGTGGAAGTGATAAAGCCAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑Tc                                                                                                                                                                                                                                                                                                  <  2:809497/289‑1 (MQ=25)   ggCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGCAGTG‑TCTg                                                                                                                                                                                                                                                                                                >  2:28405/1‑289 (MQ=21)    gCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTgg                                                                                                                                                                                                                                                                                               >  2:279554/1‑289 (MQ=34)        ccAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGtt                                                                                                                                                                                                                                                                                             >  1:67439/1‑287 (MQ=34)        ccAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTc                                                                                                                                                                                                                                                                                           >  2:202500/1‑289 (MQ=34)        ccAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTc                                                                                                                                                                                                                                                                                           <  1:679471/289‑1 (MQ=34)                               ctgttgGAAGTGATAAAGACAAAAAGGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCtgttgt                                                                                                                                                                                                                                                                    <  1:417053/285‑1 (MQ=25)                               cTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCtgttgt                                                                                                                                                                                                                                                                    >  1:100509/1‑289 (MQ=35)                                   tgGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAATTGTTCTGG‑TTCAAGATTAGCCCCCGTTCtgttgt                                                                                                                                                                                                                                                                    >  1:820292/1‑285 (MQ=21)                                   tgGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAATTGTTCTGG‑TTCAAGATTAGCCCCCGTTCtgttgt                                                                                                                                                                                                                                                                    <  2:820292/285‑1 (MQ=21)                                   tgGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAgg                                                                                                                                                                                                                                                                >  2:629066/1‑289 (MQ=35)                                   tgGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTCTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGCAATAATTCCCGCTAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGACATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCATGATTAGACCCCGTTCTGTTGTCAgg                                                                                                                                                                                                                                                                >  2:276512/1‑289 (MQ=255)                                       aGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGtttt                                                                                                                                                                                                                                                            <  2:100509/289‑1 (MQ=35)                                             aaaGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑Cctc                                                                                                                                                                                                                                                      >  2:137800/1‑288 (MQ=34)                                                     aaaTGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATTACAACATTTTGCGGCTATTCTTGAATTG‑TCTGGTTTCAAGTTTTGCCCCCGTTCTGTTGTCAGGTTTTA‑CTTCCCAACGttg                                                                                                                                                                                                                                             >  1:499193/1‑287 (MQ=255)                                                                   tGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑Tctct                                                                                                                                                                                                                               <  2:151473/288‑1 (MQ=34)                                                                       aCCCGTGGGGGCGGTGTCGGGCGCATATTGCCATCACGGAAGCCTGACGCCCGTTTTCACCTTACTTGCGGTTACGCCACCAGCCGACAATCGCTGCGGTGATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCTGCTTTTTTTTAATGACAACATATCGCGGCTATTCTTGAGCTG‑TCTGGTTTCAGGATTAGACGTCTATAAGTTGCAAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCa                                                                                                                                                                                                                          <  1:540610/289‑1 (MQ=11)                                                                         cccTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTGTTCAGGGTTTA‑ACTCGCAAGGTGTGGGGGTTTATCT‑TTTCCAg                                                                                                                                                                                                                         >  1:552097/1‑288 (MQ=11)                                                                             gCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATCTAGCCGCCTTGTTGTAATGACAACATTTTTCGGCTATTCCTTAAGTG‑TCTGGTTTCAAGGTTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTATCAACGTTGCGGGGTCT‑TCTTTTTACAggcacc                                                                                                                                                                                                                    >  1:504979/1‑284 (MQ=11)                                                                                   ggTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTTCGGCTTTTCTTGAAGTT‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCCCAACGTGCGGGGGTTT‑TCCCTTTCCAGCACCAAATGc                                                                                                                                                                                                               >  1:382357/1‑288 (MQ=255)                                                                                      ttCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGccac                                                                                                                                                                                                            <  1:286860/288‑1 (MQ=34)                                                                                       tCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGccacca                                                                                                                                                                                                          <  2:67439/289‑1 (MQ=34)                                                                                              cATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATaa                                                                                                                                                                                                   >  1:334201/1‑289 (MQ=34)                                                                                               atatTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATaaa                                                                                                                                                                                                  <  2:100433/289‑1 (MQ=34)                                                                                                tatTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAAt                                                                                                                                                                                                                 >  2:515486/1‑273 (MQ=34)                                                                                                tatTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAAt                                                                                                                                                                                                                 <  1:515486/273‑1 (MQ=34)                                                                                                 atTGCCATCACGGCAGCCTGCCGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGc                                                                                                                                                                                                <  2:504979/289‑1 (MQ=21)                                                                                                         cACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGACGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCa                                                                                                                                                                                         >  2:462916/1‑288 (MQ=25)                                                                                                         cACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCa                                                                                                                                                                                         >  2:211110/1‑288 (MQ=35)                                                                                                           cGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACa                                                                                                                                                                                      >  2:504245/1‑289 (MQ=37)                                                                                                               aGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTgc                                                                                                                                                                                  >  2:657108/1‑289 (MQ=37)                                                                                                                 ccTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGTGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTgcg                                                                                                                                                                                 >  2:601978/1‑288 (MQ=37)                                                                                                                  cTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTgcg                                                                                                                                                                                 <  2:847469/287‑1 (MQ=37)                                                                                                                      cGCCCGTTTTCACCTTACTTCCGTGTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCAc                                                                                                                                                                           <  2:552097/289‑1 (MQ=37)                                                                                                                      cGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGCGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCAc                                                                                                                                                                           <  2:240608/289‑1 (MQ=37)                                                                                                                         ccGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCgg                                                                                                                                                                        <  2:499193/289‑1 (MQ=37)                                                                                                                                    ttACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGc                                                                                                                                                             <  1:297021/289‑1 (MQ=39)                                                                                                                                    ttACTTCCGGTTAATCCACCAGCAGACAAGCGCTGCGGTAATAAGTCCCGCCAGGATCGGTGCGGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTCTGA‑CCTCTCAACGTGCGACGCTCT‑GCATATTGCAGCCACCGTCGCAACTGTAGATGAAGCCGCCGCAACATTGCGCCTCACCGGATAATTCCGGc                                                                                                                                                             <  1:206889/289‑1 (MQ=11)                                                                                                                                      ccTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCtt                                                                                                                                                           <  1:504245/288‑1 (MQ=39)                                                                                                                                      aCTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCtt                                                                                                                                                           <  1:202500/289‑1 (MQ=39)                                                                                                                                         tCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGgt                                                                                                                                                        <  1:137800/289‑1 (MQ=39)                                                                                                                                         tCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGgt                                                                                                                                                        <  1:279554/289‑1 (MQ=39)                                                                                                                                         tCCGATTACGACACCAGCCGACAATCTCTGCCGTAATAAGTCCCGCCAGGAACGGTGCCGCCAGGTCGTGCCAGAAAATCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTTAATTG‑TCTTGGGTCAAGAGTAGCCCCCGTTCTGTTGTCATGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCCCCGGATAATTCCGGCTTGgt                                                                                                                                                        <  1:40701/289‑1 (MQ=11)                                                                                                                                                 ccccaccaTCCTACAATGGGTGCGGTAATAATTCCCGCCAGGAGCGGGGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGCATTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGAtac                                                                                                                                                <  1:276512/287‑1 (MQ=255)                                                                                                                                                           cGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCaa                                                                                                                                      <  1:318145/289‑1 (MQ=39)                                                                                                                                                                                  cccGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGaa                                                                                                               >  2:806371/1‑289 (MQ=39)                                                                                                                                                                                    cGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTg                                                                                                                                                                                                                                              >  2:4834/1‑161 (MQ=35)                                                                                                                                                                                    cGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTg                                                                                                                                                                                                                                              <  1:4834/161‑1 (MQ=35)                                                                                                                                                                                       cAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGt                                                                                                           <  2:382357/288‑1 (MQ=39)                                                                                                                                                                                       cAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTCCATCCCTGAAATGt                                                                                                           <  1:657108/288‑1 (MQ=39)                                                                                                                                                                                            tCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCa                                                                                                      <  2:764352/288‑1 (MQ=39)                                                                                                                                                                                            tCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCa                                                                                                      <  1:806371/288‑1 (MQ=39)                                                                                                                                                                                            tCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCa                                                                                                      <  2:22935/288‑1 (MQ=39)                                                                                                                                                                                                   cgccAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAaga                                                                                               <  1:445159/288‑1 (MQ=39)                                                                                                                                                                                                     ccAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGgt                                                                                                                                                        <  2:697272/229‑1 (MQ=39)                                                                                                                                                                                                     ccAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGgt                                                                                                                                                        >  1:697272/1‑229 (MQ=39)                                                                                                                                                                                                          tCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCACCATTGCGCCTCACCGGATAATTCCGCCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAACGTTTCCAATAAAGAGTACAtt                                                                                        >  2:75644/1‑288 (MQ=255)                                                                                                                                                                                                                    aaGTAATGGCAGACTGCGCAAAGGTAATATAGCCGCCTTGTTGTAATGACAACATTTTGCGCGTAGTCTTGAAGTG‑TGTGGATGCACGATCAGACCCCGTACTGGTGTCACGTTTTA‑GCTCTCAACGAGCAGTGTTTC‑TTTCTCGGCAGCAACAAATGACCCCAGGGATAAGGCCCACGAAACATTGCGCGTCACACGATAATTCCGCCTTGGATAGCATAGTCCGCGTCAGCTGTGTATAAGTGCCTGCCTGAAATGTGTGCAATAAAGAGTACATTCCGGCgtt                                                                                <  1:194669/286‑1 (MQ=2)                                                                                                                                                                                                                         aTGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGc                                                                                   <  2:265696/278‑1 (MQ=38)                                                                                                                                                                                                                         aTGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGc                                                                                   >  1:265696/1‑278 (MQ=38)                                                                                                                                                                                                                             cAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCaa                                                                                                                                                                                                                  >  1:622749/1‑147 (MQ=32)                                                                                                                                                                                                                             cAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCaa                                                                                                                                                                                                                  <  2:622749/147‑1 (MQ=32)                                                                                                                                                                                                                                aCTGCGCGAGCGTCCTATAGCAGCGTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCa                                                                 <  1:629066/289‑1 (MQ=255)                                                                                                                                                                                                                                   gcgcGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAATTGTTCTGG‑TTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAg                                                                         <  1:340853/278‑1 (MQ=12)                                                                                                                                                                                                                                   gcgcGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAATTGTTCTGG‑TTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAg                                                                         >  2:340853/1‑278 (MQ=12)                                                                                                                                                                                                                                             cATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGa                                                     <  1:211110/288‑1 (MQ=38)                                                                                                                                                                                                                                                       ccTTGTTGTAATGACAACATTTTGCGGCTATTCTTTAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCACTGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCCTGAGTGCCCTGgat                                          >  2:552543/1‑289 (MQ=255)                                                                                                                                                                                                                                                       ccTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTTTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGgat                                          >  2:228467/1‑289 (MQ=255)                                                                                                                                                                                                                                                       ccTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGgat                                          >  2:252970/1‑289 (MQ=255)                                                                                                                                                                                                                                                              gtAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCt                                    >  2:492182/1‑288 (MQ=255)                                                                                                                                                                                                                                                                    actagatgtgtccgcgatgcTTGAAGTG‑TCTGGTTTCAAGATTAGCCCGCGTTCTGTTGTCAGGTTTGA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAAGTGATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCGTGGTGTGGATACTACGTCTCAATTCATCTTCTCTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCCGTTCAACGGGTGTGGCGGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCt                             <  1:28405/270‑1 (MQ=14)                                                                                                                                                                                                                                                                      aacaTTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCg                           >  1:183235/1‑289 (MQ=255)                                                                                                                                                                                                                                                                       acaTTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCgg                          <  1:601978/289‑1 (MQ=255)                                                                                                                                                                                                                                                                        caTTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAATCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGATTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCgg                          <  2:858277/289‑1 (MQ=255)                                                                                                                                                                                                                                                                            tttcGGCTATTCTTAAAGTG‑TCTGGTTTCAAGATTACCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGc                     <  1:75644/286‑1 (MQ=255)                                                                                                                                                                                                                                                                             tGCGGCTATGCTTGAAGTG‑TCTGGTTTCAAGTTTATCCCCCATTCTGTAGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGcc                    <  1:552543/289‑1 (MQ=255)                                                                                                                                                                                                                                                                                               g‑tCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCa                                                                                                                                                                                                                                                   >  2:731183/1‑49 (MQ=38)                                                                                                                                                                                                                                                                                               g‑tCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGaa   <  1:462916/288‑1 (MQ=255)                                                                                                                                                                                                                                                                                               g‑tCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAag  >  1:68693/1‑289 (MQ=255)                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                   GAGGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAGTGATAAAGACAAAAATGGCCGCAGGCTGTTACCCCTGCGGCCGGTTTCGGGCGCATATTGCCATCACGGCAGCCTGACGCCCGTTTTCACCTTACTTCCGGTTACGCCACCAGCCGACAATCGCTGCGGTAATAATTCCCGCCAGGATCGGTGCCGCCAGGTCGTGCCAGAAAGTCATGGCAAACTGCGCGAGCGTCATATAGCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTG‑TCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTA‑CCTCTCAACGTGCGGGGGTTT‑TCTCTTTCCAGCAACCAATGCCACCAGGGATAAAGCCCCCGCAACATTGCGCCTCACCGGATAATTCCGGCTTGGTGTGGATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTTACTTGTGGAAG  >  NC_000913/1269572‑1270146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑