Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,769,856 T→A 33.3% intergenic (+74/‑91) yibV → / → yibU pseudogene, putative cryptic Rhs immunity protein/pseudogene, H repeat‑associated protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,769,8560TA33.3% 9.6 / 8.5 12intergenic (+74/‑91)yibV/yibUpseudogene, putative cryptic Rhs immunity protein/pseudogene, H repeat‑associated protein
Reads supporting (aligned to +/- strand):  ref base T (6/2);  new base A (2/2);  total (8/4)
Fisher's exact test for biased strand distribution p-value = 5.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.20e-01

TATCTGAAAAATCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGAGAGCACACTCATCCAGAACTTATTAACA  >  NC_000913/3769590‑3770142
                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                              
tatCTGAAAAATCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTgaagaag                                                                                                                                                                                                                                                                          >  1:819907/1‑289 (MQ=255)
                                                                                                                   gatgatAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGta                                                                                                                                                                                                                                                              <  1:182882/186‑1 (MQ=255)
                                                                                                                   gatgatAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGta                                                                                                                                                                                                                                                              >  2:182882/1‑186 (MQ=255)
                                                                                                                                                                  tgatgaTTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGaacaac                                                                                                         >  2:101741/1‑288 (MQ=255)
                                                                                                                                                                           tataAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATatcatc                                                                                               >  1:652660/1‑289 (MQ=255)
                                                                                                                                                                                                                                        tcCAGGAGTTTTTTTAACTTAGTTTTATAATGAAAACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCt                                                               <  1:616950/260‑1 (MQ=255)
                                                                                                                                                                                                                                        tcCAGGAGTTTTTTTAACTTAGTTTTATAATGAAAACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCt                                                               <  1:616954/260‑1 (MQ=255)
                                                                                                                                                                                                                                        tcCAGGAGTTTTTTTAACTTAGTTTTATAATGAAAACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCt                                                               >  2:616950/1‑260 (MQ=255)
                                                                                                                                                                                                                                        tcCAGGAGTTTTTTTAACTTAGTTTTATAATGAAAACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCt                                                               >  2:616954/1‑260 (MQ=255)
                                                                                                                                                                                                                                          cAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAAt                                >  2:290117/1‑289 (MQ=255)
                                                                                                                                                                                                                                                            aGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGAGAGCACACTCATCCAg              <  2:819907/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                        aaTACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGAGAGCACACTCATCCAGAACTTATTAACa  >  1:616042/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                                                              
TATCTGAAAAATCTAAATCCACTCCAACTTGCCGCTGATTGCATTGAAACAGTAAATAAAATAAAAGATAAGAATAAAAAAAATCATTGATATAAATTCAGTTAATATTTGTAGTGATGATAAAAATATAAAATTGAGAGTTAATAGTACTATTATGGCCATTGATGATTCTATAAAGTGCATCGATGAATGATAACTTAATTTTATTTAAAAATATATTAATAACTCACTCTCCAGGAGTTTTTTTAACTTAGTTTTATAATGAATACGATAATTAAAAGTGAAGAAGAAAATAGTTGTATACATATATTCCATGACCCTACATTGTGATCTATAGATTAAATATTTTTTAAATGTGACTGTCATCCTTCAGATAATAAAGCCATCATCGCAATAGATGGAAAAGCACTCCTGCTCTTTTTTGATTAGAGCCGTCGCAGGAGGAACAACTCATATCATCATTGCGATCTCGACGATGTATAGTCTCATCCTGCGGCAGAACAAGACGGATAAAAAATCCAATGAGAGCACACTCATCCAGAACTTATTAACA  >  NC_000913/3769590‑3770142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: