New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 30683440 (0.950)3 (0.070) 3/272 6.2 7.4% coding (2499/2526 nt) ecpC ECP production outer membrane protein
?NC_000913 = 306863 37 (0.920)coding (2470/2526 nt) ecpC ECP production outer membrane protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

TTCGACAAACACAAACTCGCGTGCCGGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCA  >  NC_000913/306692‑306976
                                                                                                                                              |                                                                                                                                              
ttCTACAGACACAAACTCGCGTGCCGGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGCGCGCATTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGc                                                                                                                                                <  2:439729/143‑1 (MQ=255)
      aaacacaaacTCGCGTGCCGGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTTCGCCAcc                                                                                                                                          >  1:71587/1‑143 (MQ=255)
      aaacacaaacTCGCGTGCCGGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGACTGCGTCAcc                                                                                                                                          >  1:490617/1‑143 (MQ=255)
       aacacaaacTCGCGTGCCGGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCAccg                                                                                                                                         <  1:5006/143‑1 (MQ=255)
          acaaacTCGCGTGCCGGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTATTCTCCTCTCTGCGTCAccgccg                                                                                                                                      >  1:451208/1‑143 (MQ=255)
              acTCGCGTGCCGGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGGTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCa                                                                                                                                  >  2:81989/1‑143 (MQ=255)
                tCGCGTGCCGGTGCATCCGCCCAGGTTGTTTTGCTCATTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACg                                                                                                                                >  2:22660/1‑143 (MQ=255)
                tCGCGTGCCGGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTACCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACg                                                                                                                                >  2:371158/1‑143 (MQ=255)
                    gTGCCGGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATga                                                                                                                            <  2:719857/143‑1 (MQ=255)
                        cGGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAgg                                                                                                                        <  1:11742/143‑1 (MQ=255)
                         ggTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGc                                                                                                                       <  2:563473/143‑1 (MQ=255)
                                  gCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGTGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCACCCACGATGAGAGGCCGCTGCAGa                                                                                                              <  2:38869/143‑1 (MQ=255)
                                    ccAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTCTCATCGTTATTAGTAGGTTAACACTCATTCTCATCTCCTGACTGCGACACCGCCGCACACGACGAGAGGCCGCTGCAGAcc                                                                                                            >  1:397096/1‑143 (MQ=255)
                                       ggTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCCCCGCCCACGATGAGAGGCCGCTGCAGAccccc                                                                                                         >  2:553863/1‑140 (MQ=255)
                                              ttGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGa                                                                                                  <  1:107615/143‑1 (MQ=255)
                                               tGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAAATCTCATCGTGAGTAGTAGGTTAACGCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGAcaggagaagagaa                                                                                                 <  2:435669/143‑13 (MQ=255)
                                                      tGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGGGGCCGCTGCAGACCACATCACCGACCCAGACg                                                                                          >  1:529321/1‑143 (MQ=255)
                                                         cGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCACCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCa                                                                                       >  1:473515/1‑143 (MQ=255)
                                                                aGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGCGGCCGCTGCAGACCACAGCACCGACCCAGACGGCCCcgcgcg                                                                                >  2:536029/1‑143 (MQ=255)
                                                                   tcAGTTACTGGCCAGATTAGCGCGAATATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCGCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCt                                                                             <  1:344797/142‑1 (MQ=255)
                                                                   gcAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGACTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGGGCGCCt                                                                             >  1:659868/1‑143 (MQ=255)
                                                                     aGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTgg                                                                           >  2:329377/1‑143 (MQ=255)
                                                                                aGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTcccg                                                                >  1:686025/1‑141 (MQ=255)
                                                                                       cgcgAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACt                                                         >  1:537352/1‑143 (MQ=255)
                                                                                         cgAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCCGGTTGAGTTCCAGAGCCACTTc                                                       >  1:264947/1‑143 (MQ=255)
                                                                                                   cagcggtgtgagtcgGTTAACTCTCATTCTCTTCGCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTa                                             <  2:343984/133‑1 (MQ=255)
                                                                                                      cGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGCGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGCGTTCCAGAGCCACTTCGCCAGTTTCATTg                                          >  2:423415/1‑143 (MQ=255)
                                                                                                        ttattaGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCCCCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGcc                                        >  1:366153/1‑143 (MQ=255)
                                                                                                         tagtagtagGGTAGCTCTCTTTCTCGGCGCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCa                                       <  2:160416/140‑1 (MQ=255)
                                                                                                          attaGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCAc                                      <  1:85688/143‑1 (MQ=255)
                                                                                                           ttaGTCGGTTTACTCTCATTCTCTCCTCCTTTCTGCGTCACCGCCGCCCACTATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACTGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACt                                     <  2:273652/143‑1 (MQ=255)
                                                                                                            tagtagGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGACGTGGCAGCTCGGCAGACCGCATCCCCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTg                                    >  2:323159/1‑143 (MQ=255)
                                                                                                            tagtagGTTAACTCTCATTCTCCTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTg                                    <  1:22660/143‑1 (MQ=255)
                                                                                                                    tAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGCTTTCTTGACACTGTAGCGaaa                            >  1:37529/1‑143 (MQ=255)
                                                                                                                        tctcATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTCCGCAGGTTTTAGTGCCACTGGAGCGAAAATCg                        >  2:30628/1‑143 (MQ=255)
                                                                                                                          tcATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGAt                      <  1:371158/143‑1 (MQ=255)
                                                                                                                            aTTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGCAGCGCAAATCGATAg                    >  2:641189/1‑143 (MQ=255)
                                                                                                                               ctcttctcCTGTCGGCGTCGCCGCCGAACTCGAGGAGGTGCCGCTGCAGGCCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTgg                 <  2:7150/143‑1 (MQ=255)
                                                                                                                                tctgctcCTGTCTGCGTCACCGCCGACCACGCCGAGAGGCGGCTGCGGTCCCCATCAGCGCCCCAGGCGTCACCGCGCGCCTGGTTGAGTGCCAGAGCTACTTCGCAGGTTTTATTGTCACTGTAGCGAAAATCTATAGTggg                <  2:413744/143‑1 (MQ=255)
                                                                                                                                tctccgccTGTCTGCGTCACCGCCGCCCAGGATGAGAGGTCGCGGCAGACCACATCGCCGCCCCAAACGGCACCGCGCGCCTGGGTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTggg                <  1:650969/137‑1 (MQ=255)
                                                                                                                                           cTGCGTCCCCGCCGCCCGCGATGAGGGGCGGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCGGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTAt     <  2:21061/143‑1 (MQ=255)
                                                                                                                                              cGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGCCGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGATTTATTGCCACTGTAGCGAAAATCGAAAGAGGGGAATTTCTTATcga  >  1:487614/1‑141 (MQ=255)
                                                                                                                                              |                                                                                                                                              
TTCGACAAACACAAACTCGCGTGCCGGTGCATCCGCCCAGGTTGTTTTGCTCACTGCCGCTCTGAGCGCAGTTACTGGCCAGATTAGCGCGAAAATTATCATCGTTATTAGTAGGTTAACTCTCATTCTCTTCTCCTGTCTGCGTCACCGCCGCCCACGATGAGAGGCCGCTGCAGACCACATCACCGACCCAGACGGCACCGCGCGCCTGGTTGAGTTCCAGAGCCACTTCGCAGGTTTTATTGCCACTGTAGCGAAAATCGATAGTGGGGTATTTCTTATCCA  >  NC_000913/306692‑306976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.