Predicted mutation
evidence seq id position mutation annotation gene description
MC JC NC_000913 257,908 Δ776 bp [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 257908 258683 776 28 [0] [2] 30 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579070 (0.000)26 (0.990) 24/268 0.1 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers

GTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTG  >  NC_000913/258542‑258824
                                                                                                                                              |                                                                                                                                            
tgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaa                                                                                                                                              <  1:264388‑M2/1‑1 (MQ=255)
  ttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGt                                                                                                                                            >  1:277601‑M2/141‑143 (MQ=255)
              taaattttgccaatttgagaaaacagtgacatgacgacaggagagagcaagggcgttaccgagttgacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATc                                                                                                                                <  1:23653‑M2/15‑1 (MQ=255)
                      ggcatcgtagtacaaaagttgcagcaaaacaggagatagtaaggaggttagcgagtggacacgcgaagagcagattgatcaaaaaatgtaccgtactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCttt                                                                                                                        <  2:467333‑M2/23‑1 (MQ=255)
                        caatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaacgagtggacacccgaagagcagattgatcaaaaaactcaccgacctaggcccgtataatcgtggaggaaAGTGCAACGACAAGAGATTattttg                                                                                                                      >  2:367374‑M2/119‑142 (MQ=255)
                                 aaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCt                                                                                                             >  2:23297‑M2/110‑143 (MQ=255)
                                 aaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCt                                                                                                             <  1:416094‑M2/34‑1 (MQ=255)
                                         tgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaacataccccactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTAt                                                                                                     <  1:9013‑M2/42‑1 (MQ=255)
                                           catcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGc                                                                                                   >  2:131537‑M2/100‑143 (MQ=255)
                                                caacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCaa                                                                                              <  1:266857‑M2/49‑1 (MQ=255)
                                                   caggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGt                                                                                           <  2:37630‑M2/52‑1 (MQ=255)
                                                    aggagagagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTg                                                                                          <  2:185323‑M2/53‑1 (MQ=255)
                                                                             tggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCACAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAAt                                                                 >  2:336668‑M2/66‑143 (MQ=255)
                                                                             tggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAAt                                                                 >  1:462659‑M2/66‑143 (MQ=255)
                                                                             tggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGAATCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGAGCGtgagt                                                                 >  2:398934‑M2/66‑141 (MQ=255)
                                                                                 cacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTg                                                             <  1:137876‑M2/82‑1 (MQ=255)
                                                                                  acccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTgg                                                            <  1:49864‑M2/83‑1 (MQ=255)
                                                                                               attgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggatgg                                               >  1:310519‑M2/48‑143 (MQ=255)
                                                                                                   atcaaaaaatttaccgcactaggccagcacaagcgtgaagggaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGAGGGAGCt                                           >  2:149532‑M2/44‑143 (MQ=255)
                                                                                                    tcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTg                                          >  1:239957‑M2/43‑143 (MQ=255)
                                                                                                                        ggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGCAGCGCAGGAATCCCGttt                      >  1:134426‑M2/23‑143 (MQ=255)
                                                                                                                          cctgcatattcgtgagggtaAGTGCAAAGAGAGTCGATTCTCTTTCGATTGTCTGGCTGTATGCGGCAACGTGAAACCGGCACCGGAAGTGCGTGAGTTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTa                    <  2:235973‑M2/123‑1 (MQ=255)
                                                                                                                           ccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTTTTTTTTTTTGCTGTATGCGTCAACGTGAAACCGTCACCGGAAGTGCGTGAGTTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTAc                   >  2:269914‑M2/20‑143 (MQ=255)
                                                                                                                                ttttcttgtaGGTATTTGCAATGATTATCTATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGTACCGGAAGTGCGTTAATTCTGGGGCTTGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACa              <  2:252856/134‑1 (MQ=255)
                                                                                                                                tattcgtgaaggtaAGTGCAAAGATAACCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACa              <  1:23297‑M2/129‑1 (MQ=255)
                                                                                                                                     gtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTAc         <  1:131537‑M2/134‑1 (MQ=255)
                                                                                                                                     gtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTAc         >  1:393476‑M2/10‑143 (MQ=255)
                                                                                                                                      tGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTAAACGTGAAACAGGCGCCGGAAGTGCGTGAAGCCTGGGGCTGCTGGGGGGAGCCGGCAGCGCCGGAACCCCGCTTCACCCACAGTTGcc        >  2:283778/1‑143 (MQ=255)
                                                                                                                                       gaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTGTTACCTACAGTTaccg       >  1:295858‑M2/8‑142 (MQ=255)
                                                                                                                                            taAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTAGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGATTTACCTCCAGTTACCAGCTTg  >  2:165898‑M2/3‑143 (MQ=255)
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GTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTG  >  NC_000913/258542‑258824

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: