Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC NC_000913 257,908 Δ776 bp 100% [crl] [crl]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 257908–258675 258683 9–776 74 [0] [0] 73 [crl] [crl]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2579070 (0.000)66 (1.280) 53/392 NT 100% intergenic (+8/‑769) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 258684 = 0 (0.000)pseudogene (9/331 nt) crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers

GTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGT  >  NC_000913/258462‑258907
                                                                                                                                                                                                                              |                                                                                                                                                                                                                                 
taggaatcccatttaccgccgtgtatcggaattttgacaaaggtctttaggaaataaccgactagatcgaaaaacgcttgtggtaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAgtgga                                                                                                                                                                                                                              <  1:443356‑M2/6‑3 (MQ=255)
    gttcccatttaacccggtttttcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGa                                                                                                                                                                                                                          <  1:325951‑M2/10‑1 (MQ=255)
       cccatttaacccggtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagaagattgatcaaaaaatttaccgaactaggcccgtatattcgggaaggaaagtgcaaaaaaaa                                                                                                                                                                                                                       >  1:569052‑M2/216‑223 (MQ=255)
                    gtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcccaaaaggcgaaagcaatgccgttaccgagtggacacccgaagagcagattgatcaaaaaatttacacctatagggccgtatatttgggaaggtaAgtgcaaacaaaataaacagtccctt                                                                                                                                                                                                          >  1:268707‑M2/203‑216 (MQ=255)
                    gtgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggaaacccgaagagcagattgatcaaaaaatttaccgcactaggcccgaagattcgtgaaggaaAGAGCAAAATTAAACGATTctttttg                                                                                                                                                                                                          >  1:677707‑M2/203‑227 (MQ=255)
                     tgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagccgattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggaaAGTGCAAAGATAATCGATTCtttt                                                                                                                                                                                                            >  2:451953‑M2/202‑225 (MQ=255)
                     tgtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtggtaaaaatttgctaaattgtgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCa                                                                                                                                                                                                                              <  2:268707‑M2/6‑1 (MQ=255)
                     tgaatcggaattttgacaaaggtcttcaggaaatcaccgactggatggaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcaaaaaaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcaataggcagggagattcgtgaaggtacgtgcaaagactgactctttaaaccat                                                                                                                                                                                                         >  2:583597‑M2/202‑211 (MQ=255)
                      gtatcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGa                                                                                                                                                                                                        <  2:569052‑M2/28‑1 (MQ=255)
                         tcggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgaatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcggattgatcaaaaaatttaacgccctaggcccgtatattcgtgaaggaaAGTGCAAAGAGAATCGATTCTTTTTCGAtt                                                                                                                                                                                                      >  2:532016‑M2/198‑227 (MQ=255)
                          cggaattttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGt                                                                                                                                                                                                    >  2:454422‑M2/197‑228 (MQ=255)
                          cggaattttgaaaaagggtttcaggaaattaccgactggatcgaaaaacggttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGt                                                                                                                                                                                                    <  1:598448‑M2/32‑1 (MQ=255)
                                 ttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacatgagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgccctaggcccgcaaattcgtgaaggtaAGTGCAAAGAAAATCGATTATTTTTCGATGGTTTGGCTg                                                                                                                                                                                             >  1:427124‑M2/190‑228 (MQ=255)
                                 ttgacaaaggtcttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaactttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtattttcgtgaaggacAGTGCAAAGATAATCGATTCTTTTTCGCTTGTCTGGCTg                                                                                                                                                                                             >  2:18608‑M2/190‑228 (MQ=255)
                                             ttcaggaaatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgcatattcttgaaggtaAGTGGAAAGATAATCGATTCTTTTTCGATTttctggctgtagacgtaaaag                                                                                                                                                                                 >  1:630063‑M2/178‑218 (MQ=255)
                                                 agaaatccccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGaa                                                                                                                                                                            <  2:162398‑M2/55‑1 (MQ=255)
                                                    aatcaccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAAcc                                                                                                                                                                         >  2:86297‑M2/171‑228 (MQ=255)
                                                      tccccgactggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAAcc                                                                                                                                                                         <  2:335448‑M2/58‑1 (MQ=255)
                                                            actggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagcttgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGAGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACAGTGACCgg                                                                                                                                                                 >  2:702889‑M2/163‑228 (MQ=255)
                                                            actggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgggaaggtaAGTGCAAAGATAATCGATTTTTTTTTGGTTTTCTGGCTGTATGCGTCAACGTGAAACCCGCCCCgg                                                                                                                                                                 >  1:416244‑M2/163‑228 (MQ=255)
                                                              tggatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGGTTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGa                                                                                                                                                                <  2:416244‑M2/67‑1 (MQ=255)
                                                               gatttaaaaaacgcttttgttaaaaatttgttaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaattacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAg                                                                                                                                                              <  1:629833‑M2/69‑1 (MQ=255)
                                                                gatcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaaccggagatagcaatgacgttaccgagtggacacccgacgagaagattgatcaaaacatttaccgccatcggcccgtatattcgtgaaggtaCGTCCAAAGATACTCGTTTCTTTTTCGGTTGCCTGGCTGTATGCGTCACACGAAAACAGGCACCGGa                                                                                                                                                                >  2:404389‑M2/159‑225 (MQ=255)
                                                                 atcgaaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTg                                                                                                                                                            >  2:599837‑M2/158‑228 (MQ=255)
                                                                     aaaaacgcttgtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGGGAAACCGGAACCGGAAGTgcggg                                                                                                                                                        >  2:566548‑M2/154‑226 (MQ=255)
                                                                               gtgttaaaaatttgctaaattttgccaatttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTgggg                                                                                                                                              >  2:379165‑M2/144‑228 (MQ=255)
                                                                                        atttgttaaattttgccaatttggtaaaacagtggcatcgaaacaggagatagaaatgccgttacgaggtggacaccggaagagcagattgaacaaaaaatttaccgcactaggctcgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTGTGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggat                                                                                                                                     <  1:333147‑M2/94‑1 (MQ=255)
                                                                                         tttgctaaattttgccaatttggaaaaacagtttgatcacaacaggagatagcaatgatgttgccgagtggacacccgaagaacagattgatctaaaaatttaccgcactaggcccgtttattcgtgaaggtaATTGCAAAGCTAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGACAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGtggat                                                                                                                                     <  2:435940‑M2/94‑1 (MQ=255)
                                                                                           tgctaaattttgccaatttggtaaaacagttgcatcacaacaggagataggaatgacgttaccgagtggacacacgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTAAACGTGAAACCGGCACCGGCAGTGCGTGAATTCTGGGGCTGGgggctgga                                                                                                                                  >  2:632954‑M2/132‑228 (MQ=255)
                                                                                                   tttgccaatttgggaaaacagttccaacacaacaggagatagcaagcgcgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgtactaggcccgtttattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTGTCGATTGTCTGGCTGTATGCGGCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAg                                                                                                                          <  1:306980‑M2/105‑1 (MQ=255)
                                                                                                     tgccaatttggtaaaacagttgcatcacaacaggagatagcaattacgttaccgagtggacacccgaagagtagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAgcg                                                                                                                        <  2:111702‑M2/107‑1 (MQ=255)
                                                                                                         aagttggtaaaacagttgcatcacaacaggagatagcaatgacgttaccgagttgacccccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAg                                                                                                                     <  2:419819‑M2/110‑1 (MQ=255)
                                                                                                           tttggtaaaacagttgcatcacaacaggatatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGaa                                                                                                                  <  2:165018‑M2/113‑1 (MQ=255)
                                                                                                             tggtaaaaaagtggcatcaaaacaggaaatagcaatgtcgttaccgagtggacatccgaagagcagagtgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTTGTGGATGGAGCTGGAAGCGCAGGAATc                                                                                                                <  1:532016‑M2/115‑1 (MQ=255)
                                                                                                                 aaaacagttgcatcaaaaagagagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGGCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGt                                                                                                            <  1:97001‑M2/119‑1 (MQ=255)
                                                                                                                               acaacggagaatagcaaagcggttaccgagtggcaacaaggagagcacagtgatcaaaaaatgtaccgcactaggctcgtatagtcgggaaggtaAGTGCAAAGATAATCGATTCTTTTTCGAGTGTCTGGCTGGATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGGGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTa                                                                                              <  1:566548‑M2/133‑1 (MQ=255)
                                                                                                                                gagacagaaatagaaatgacgttcccgatgggacacacgaagagcagattgatcaaaaaatttaccgccataggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTa                                                                                              <  2:701942‑M2/133‑1 (MQ=255)
                                                                                                                                caacaggagatagcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTc                                                                                                                                                   >  2:184243‑M2/95‑174 (MQ=255)
                                                                                                                                 aacaggagatagaaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcattaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCt                                                                                                                                                  <  1:184243‑M2/81‑1 (MQ=255)
                                                                                                                                         atagaattgctgttcccgtgtggaaacccgaagagcagattgatcaaaaaatttaccggtctaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGTTTGTCTGGCTGTATGCGTCAACGTGAAGCCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGt                                                                                    <  1:43806‑M2/143‑1 (MQ=255)
                                                                                                                                         atagaagttacgtggccgagaggacaaccgaagagaagattgaacaaaaaatttaccgcactgagcccatatatgcgtgaaggtaAGGGCAAGGATAATCGATTCTTTGTCGATTGTTTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTTAATTCTGGGTCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTGACCAGTTTGGt                                                                                    <  1:632954‑M2/143‑1 (MQ=255)
                                                                                                                                           agcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTCCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAg                                                                                                                          >  2:404535‑M2/84‑188 (MQ=255)
                                                                                                                                           agcaatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTCCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAg                                                                                                                          <  1:404535‑M2/105‑1 (MQ=255)
                                                                                                                                            gatatgtcgttccggagtggacacccgaaagggagattgaacaaaaaatttaccgcactaggcccggatagtcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTg                                                                                 <  1:275461‑M2/146‑1 (MQ=255)
                                                                                                                                            acaatggcgttcccagggggacacccgaataggagaatgaacaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTg                                                                                 <  1:379165‑M2/146‑1 (MQ=255)
                                                                                                                                             caatgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGTGGCTGGTGGATGGAGCTGGAAGGGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTgg                                                                                     >  2:43806‑M2/82‑223 (MQ=255)
                                                                                                                                                tgacgttaccgagtggacacccgaagagcagattgatcaaaaaatttaccgcactaggcccgtagattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGAATGCGTCAACGTGAAACCGGCACCGGACTATCGTGAATTCTGGGGCTGTCGGTTGTAGCTGGAAGCGCAGGAATCCCGGTTTTCCTACAGTTACCACTTTGGTCtgttgg                                                                             >  1:460959‑M2/79‑226 (MQ=255)
                                                                                                                                                  acgttaccgagtggacacccgaagagcagattgataacaaaatttaccgcactaggcccgtatattcgtgaagggaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGGGTCAACGTGAAACCGGCACCGGAAGTGCGTGAGTTCTTGGGCTGGTGGATGGAGCTGGAAGCGGAGGAATCCCGTTTTTGCTGCAGTTACCAggttggtgttgtc                                                                              >  1:701942‑M2/77‑219 (MQ=255)
                                                                                                                                                      taccgagttgacacccgaatagcagtttgaacaaaaaatttaccgcttgaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTGTCGATGGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAg                                                                       <  2:334129‑M2/156‑1 (MQ=255)
                                                                                                                                                           agtggacacccgaagagcagattgatcaaaaaatttaccgctctaggcccgtatatgcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGc                                                                  <  2:427124‑M2/161‑1 (MQ=255)
                                                                                                                                                                         gagcagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAACCGCAGGAATCCCGTTTTACCTACCGTTACCAGTTTGGTCTGGTCGATAAAGCAGGCGACTGAAAGAgtgt                                                    >  1:404716‑M2/54‑228 (MQ=255)
                                                                                                                                                                           gcaaattttccaaaaaatttaccgcactaggcccgtatattcgttaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTc                                                  <  2:316842‑M2/177‑1 (MQ=255)
                                                                                                                                                                            cagatttattaaaatatttacctcattaggcccgtatattcgggaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTAGGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTc                                                  <  1:207765‑M2/177‑1 (MQ=255)
                                                                                                                                                                            cagattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGGGTTc                                                  >  2:572825‑M2/51‑227 (MQ=255)
                                                                                                                                                                            acgattgatcaaaaaatttcccgctctgtgcccgtatatgcgtgaaggtaAGTGCAAAGATAATCGATTCGTTGTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTc                                                  <  2:677707‑M2/177‑1 (MQ=255)
                                                                                                                                                                              gattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGActggaagaggtttc                                                  >  2:460689‑M2/49‑220 (MQ=255)
                                                                                                                                                                              gattgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGGGCAAAGATAATCGCTTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTTTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTCACAGTTTGGGCTGTTCGATAAACGAGGgtcctggaagaggttaccgg                                               >  1:290370‑M2/49‑220 (MQ=255)
                                                                                                                                                                               gttgataaaaaaatgtaccgcactaggcccgtatattctggaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAaga                                                        <  2:290370‑M2/171‑1 (MQ=255)
                                                                                                                                                                               attgatcaaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATc                                                                                                                <  2:282131‑M2/115‑1 (MQ=255)
                                                                                                                                                                                      aaaaaatttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTCCAGTTACCAGTTTGGTCTGTTCGTTAAAGCAGGGGACTGGAAGAGGGTTCCGGTAAAAGac                                       >  2:516685‑M2/41‑228 (MQ=255)
                                                                                                                                                                                             ttaccgcactaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGCTGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTCCCAGTTGGGTCTGTTCGATAAAGCAGGCGGCGGGCAGAGGGTCCCGGAAAAAGAAACTGAAg                               >  1:603046‑M2/34‑228 (MQ=255)
                                                                                                                                                                                              gtccgccctaggcccgtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGaa                                <  2:603046‑M2/194‑1 (MQ=255)
                                                                                                                                                                                                        ggcccggatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGa                    <  2:630063‑M2/206‑1 (MQ=255)
                                                                                                                                                                                                          cccttattttcgtgaaggtaAGTGCAAAGATAATCGGTTCTTTTTCGAGTGTGTGGGTGTATGCGGCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACt                  <  1:572825‑M2/208‑1 (MQ=255)
                                                                                                                                                                                                             gtatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGGTAAAGCAGGCGACTGGCAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGTACGACTGGa               >  2:233253‑M2/18‑228 (MQ=255)
                                                                                                                                                                                                              tatattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGAACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGATTGGTCTGTTCGATAAAGCAGGCAACTGGAAGAGTGTTCCGGTAAAAGCAACTGAAGTGGTGGAAGGACGGGAg              >  1:582192‑M2/17‑228 (MQ=255)
                                                                                                                                                                                                                tattcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGca            >  2:177250‑M2/15‑228 (MQ=255)
                                                                                                                                                                                                                   tcgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGTTGGAGCTGGAATCGCAGGCATGCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGGAAAGGCACTTGACGTGGTTTAACAACGggtgccaac         >  2:192182‑M2/12‑224 (MQ=255)
                                                                                                                                                                                                                    cgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGCCTGTTCGCTCAAGCAGGCGACTGGAAGAGTGTTACGGGAAACGCACCTGAAATGGTTGAACGACTGGAGCACAcc        >  2:331607‑M2/11‑228 (MQ=255)
                                                                                                                                                                                                                    cgtgaaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGGGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGCCTGGGCCACAcc        >  2:498317‑M2/11‑228 (MQ=255)
                                                                                                                                                                                                                        aaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGa                    <  2:546393‑M2/206‑1 (MQ=255)
                                                                                                                                                                                                                        aaggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGa                    >  1:546393‑M2/7‑212 (MQ=255)
                                                                                                                                                                                                                          ggtaAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGCCTGGAGCACACCCGGCGt  >  2:490839‑M2/5‑228 (MQ=255)
                                                                                                                                                                                                                              |                                                                                                                                                                                                                                 
GTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATTGTCTGGCTGTATGCGTCAACGTGAAACCGGCACCGGAAGTGCGTGAATTCTGGGGCTGGTGGATGGAGCTGGAAGCGCAGGAATCCCGTTTTACCTACAGTTACCAGTTTGGTCTGTTCGATAAAGCAGGCGACTGGAAGAGTGTTCCGGTAAAAGACACTGAAGTGGTTGAACGACTGGAGCACACCCTGCGT  >  NC_000913/258462‑258907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: