| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 729,603 | G→C | 13.4% | A7A (GCG→GCC) | rhsC → | Rhs protein with putative toxin domain, putative neighboring cell growth inhibitor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 729,603 | 0 | G | C | 13.4% | 46.0 / 2.1 | 26 | A7A (GCG→GCC) | rhsC | Rhs protein with putative toxin domain, putative neighboring cell growth inhibitor |
| Reads supporting (aligned to +/- strand): ref base G (10/12); new base C (2/2); total (12/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
CAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGG‑TTCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGC > NC_000913/729399‑729810 | cAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGCAAACCGGCAGCCCGCCAAGGAGAAAAAACGCAGt > 1:41792/1‑227 (MQ=25) tacgatgAATATTTAAAAATCTTTTTACAAATCGAAATATTAATGCAGGAAATATATATAAATATTTTCCTAATGGTTCTTAGCTGACAGATATCACACATAAGGCTTTCTTATAAATCTGTAGGGTTTCGCCGGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCa < 1:328880/222‑1 (MQ=18) tCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGGAGCATTTTCCCGGG‑GTTACCCgg > 1:61128/1‑228 (MQ=255) cAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGAAGCCTTGTCCACGG‑TTCATCCGGGGGACGCa > 2:483491/1‑227 (MQ=255) aTCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGAATGGCGGGAACATTGTCCCGGGTTTCGGCCggggaagccac > 1:74072/1‑220 (MQ=18) tatatTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGG‑TTCAGCCGGGGTACGCATCGGTGCCCCCa < 2:61128/228‑1 (MQ=255) tttCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGG‑TTCAGCCGGGGTACGCATCGGTGCCCCCACCGGc < 2:187521/207‑1 (MQ=255) aTTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGGAGCCTTGTCCAGGG‑GTCAGACGGGGGACGCATCCGTGCCCCCCCCCGCGGGGCCTGTTCGGTGTGCCCCGGCggg > 2:451499/1‑227 (MQ=17) aTCTGACAGATATCTCACTTAAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCAATGTCCAGGG‑TTCAACCCGGGTACGCATCGGTGCCCCCCCCGGCGTGGGCGGTTCCGGGTGGCCCGGCgggggggacgccg > 2:699990/1‑219 (MQ=14) aCAGATATCTCACTTAAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGG‑GTCAGCCGGGGGACGCATCGGGGCCCCCCCCGGCGTGGCCTGGTCGGGGGGGCCCCGCGGGGGGACGTCCCGccc > 2:555528/1‑226 (MQ=14) gATATCTCACTTAAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGCCGCAGTATGGCGGGAACAATGTCCGCGG‑TTCTGGCCGGGGACACGCCGGGGACCCCACCCCGGCGGCCTGCTCGGGGGGGCCCCGCCGGGCGGCGTCCCGccccccc > 1:542384/1‑223 (MQ=9) ctcACTTCAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAG‑AAATGAGCGGAAAACCGGCAGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTTCAGGG‑GTCAGCCGGGGGGGGCATTGGTGCCCCCCCCGGCGTGGGCCTTTCGGGGGGGCCCGGGGGGGGGGGGGCCCGCCACCCggggctg > 1:3463/1‑223 (MQ=14) ctcACTTAAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTTGCCCGGG‑TTCAGCCGGGGAACGCATCGGGGCACCCCCCGGCGTGGCCCGTTGCGGGTGCCCCGCCGGGGGGCGCTCCGGCCACCCggccca > 2:90894/1‑224 (MQ=14) aCTTAAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGG‑TTCAGCCGGGGTACg < 1:8854/156‑1 (MQ=255) tgCTTTCTTATAAATCTGTAGGGTTTCGCCTGGCAGCAGACAAATAGCCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGG‑TTCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCgctgct < 2:63720/227‑1 (MQ=255) ggCTTTTTTAAAAATCTGTTGGGTTTCGCCTGTCAGCACGCAAATAACCCGATAACACAAGGAGGAGCAGATGAGCCGAAACCCGGCGGCGCGTAAGGGCGACATGACGCAGTATGGCGGTAGCATTGCCCAGGG‑TTCAGCCGGGGTACGCCTCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCgctgct < 1:90894/228‑1 (MQ=14) ttCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCCCGTCAGGGCGACATGACGCAGTATGGCGGTAGc > 2:147378/1‑120 (MQ=255) tCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCCCGTCAGGGCGACATGACGCAGTATGGCGGTAGCa < 1:147378/120‑1 (MQ=255) cTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGG‑TTCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGc < 2:74072/228‑1 (MQ=255) gggTTTCGCCTGTCAACAGACAAAAAACCCGAAAAAACAGGGATGAGCAGATGGGGGGAAAACCGGCGGCGCGTCAGGGCGGCCTGACGCAGTATGGCCGTAGCATTGTCCAGGG‑TTCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCg < 1:555528/228‑1 (MQ=14) tCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGG‑TTCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCa < 2:356526/178‑1 (MQ=255) cccGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGG‑TTCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTgcccggcc < 2:674505/228‑1 (MQ=255) aTGAGCAGGTGAGCGGAAACCCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGG‑TTCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCAtt < 2:145935/227‑1 (MQ=255) aTGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGG‑TTCAGCCGGGGTACGCATCGGTGCCCCCCCCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGGGACGTCCGGCCATCCGGTCCATCCGCTGCTCGGTGCCAAGGTGCTTCCCGGTGAAACCGAAATACCCCCTCCCGGCCCCCCGCCCTTCAtt > 2:241749/1‑227 (MQ=14) gtgggccgAAACCCGGCGGCCGGGCAGGGCGACATGGCGCGGTATGGCGGTGGCATTGTCCAGGG‑TTCAGCCGGGGTACGCATGGGGGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAACCCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGc < 1:699990/221‑1 (MQ=14) aggAGCGGAAAACCGGCGGGGCGTCAGGGCGAAATGACGCAGTATGGCGGTAACATTGGCCAGGG‑TTCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGc < 2:142453/226‑1 (MQ=255) | CAGGAAGATGTTCAGAGGAATATTTAGACATCGTTATACCAATCGAAACATATATTCATGAAATATATATAAATATTTTCCTAATTGTTCTTATCTGACAGATATCTCACTTAAGGCTTTCTTATAAATCTGTAGGGTTTCGCCTGTCAGCAGACAAATAACCCGATAAAACAAGGATGAGCAGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGG‑TTCAGCCGGGGTACGCATCGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATTCTCTCCCGC > NC_000913/729399‑729810 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |