Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,196,305 C→T 100% R395C (CGT→TGT)  icd → isocitrate dehydrogenase, e14 prophage attachment site, tellurite reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,196,3050CT97.8% 133.5 / ‑6.1 50R395C (CGT→TGT) icdisocitrate dehydrogenase, e14 prophage attachment site, tellurite reductase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base T (26/19);  minor base G (3/1);  total (30/20)
Fisher's exact test for biased strand distribution p-value = 6.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTTATCAAAATTTTATCAAAAAAAGTTATCAAAACCCCTCGGTAGTTTTGGGGTAGGCTGGCCGGTCAGGTGGTAGTTCTACTACTA  >  NC_000913/1196089‑1196528
                                                                                                                                                                                                                         |                                                                                                                                                                                                                                
gCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTgatgga                                                                                                                                                                                                                        <  2:383775/227‑1 (MQ=255)
      ggTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGCAAACCGTAACCTATGACTTCGAGGGTCTGATGGATAGCGCt                                                                                                                                                                                                                 >  2:215255/1‑228 (MQ=255)
      ggTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCt                                                                                                                                                                                                                 >  2:28285/1‑228 (MQ=255)
      ggTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAAATGTTAAAGGAATGGAAGGCGCAATCAACGCGAAAACCGTAACCTTTGACTTCGAGGGTCTGATGGATGgcgtc                                                                                                                                                                                                                 >  2:69546/1‑226 (MQ=255)
                  ggTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTAGGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCt                                                                                                                                                                                                                 >  1:491275/1‑216 (MQ=255)
                   gTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTa                                                                                                                                                                                                                <  2:491275/216‑1 (MQ=255)
                        gAAGGCGCCCTTTTTGAAGCCACCCACGGTCCTGCGCCGAAATAGGTCGGTCAGGACAAAGTAAATCCGGGCTCTATTATTCGCTCCGCTGAGATGATGCTGCGACACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCa                                                                                                                                                                                               <  1:69546/228‑1 (MQ=37)
                                   ttttGAAGCCCCCCCCGGTACTGCTCCGAAATAGGCCGGTAAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGa                                                                                                                                                                                    <  1:215255/227‑1 (MQ=255)
                                     ttcgagtcacccccGGGTCTGCGCCTAAATCGGCCGGGCAGGCCAAAGGAAATACGTGCTCTATTTGTCGCGCGGCTGGGATGATGCTGAGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGATTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAcg                                                                                                                                                                                  <  1:201709/221‑1 (MQ=18)
                                              cccacGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGGTCATCg                                                                                                                                                                         >  2:456913/1‑228 (MQ=255)
                                                cccGGTTCTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCGCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGa                                                                                                                                                                        <  2:7438/225‑1 (MQ=255)
                                                 ccggtatagccacgtaaTACGCGGGTCAAGACAGAGTAAACCCGGGCTCTAATATTCCCTCCCCTGATATGATGCTGCGCCACATGGTTAGGACCGAGGCGGGTGACTTAATTGTTAAAGGTATGGAAGGAGCAATCAGCGCGAAAACAGTAACCTATGGCTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGaaa                                                                                                                                                                      <  1:676697/213‑1 (MQ=255)
                                                               aaaTATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGGAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGCGTTTGGTGACGCGATCATCGAAAAAATGAAATAccta                                                                                                                                                        >  2:209881/1‑226 (MQ=255)
                                                                      ccGGTTAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTg                                                                                                                                                  <  2:642680/227‑1 (MQ=255)
                                                                                   ggTAAATCCTGGCTCTATTAATCTCTCCGAGTAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAATCCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAAc                                                                                                                                    <  2:702840/227‑1 (MQ=255)
                                                                                   aGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCAACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGAATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGCATTTGGTGACGCGATTATCGAAAACATGTAATGCCGTCGTTTTGTAACGTTATTaa                                                                                                                                     >  2:288271/1‑227 (MQ=255)
                                                                                             ggCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGTTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGATCGTaa                                                                                                                          >  2:639204/1‑228 (MQ=255)
                                                                                              gCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTGAATTTGTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTTACCTATGACTTCGAGTGTCTGATGGAGGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATg                                                                                                                                                                 <  2:33921/188‑1 (MQ=255)
                                                                                               ctctATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTACATTTATTAACGGGAGCGTAACg                                                                                                                        >  2:34564/1‑228 (MQ=255)
                                                                                                        tctctcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGGGTAACGCTCCCgttg                                                                                                               >  2:511078/1‑228 (MQ=255)
                                                                                                            tcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAACACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGttt                                                                                                            >  2:332588/1‑227 (MQ=255)
                                                                                                            tcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGACGTAGTTTGTTAAATTTATTAAAGGGGGCGAAACGCTCCCGTTGttt                                                                                                            >  1:406798/1‑227 (MQ=255)
                                                                                                              cGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGGTGtttttt                                                                                                         >  2:515906/1‑228 (MQ=255)
                                                                                                                   agagtatgCGGGGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAg                                                                                                   <  2:617410/223‑1 (MQ=255)
                                                                                                                        atgCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGAAACGACCCCGTTGTTTTTTGTTAGgctgc                                                                                              >  2:551474/1‑228 (MQ=255)
                                                                                                                          gctgcGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGCCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGATGAAATGTTCAGAGTTTGGTGCCGCGATCATCGAAACCTTGTAATGCCGAAGTTTGTTAATTTTATTAACGGGGACGACACGCTCCCGCTGTTTTTGTTTAGGTTGCTa                                                                                            >  1:443784/1‑228 (MQ=255)
                                                                                                                                                 aaGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCCATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTa                                                                                                                                               <  2:240276/155‑1 (MQ=255)
                                                                                                                                                 aaGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATAGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTa                                                                                                                                               >  1:240276/1‑155 (MQ=255)
                                                                                                                                                  aGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGGAATGCCGTAGTTTGTTAAATTTATTAACGGAAGCGTAACGCACCCGTTGTTTTTTGTTAGTCTGCTAAGTGTTATCAAAATTTTATCaaaa                                                                    >  1:504453/1‑228 (MQ=255)
                                                                                                                                                                    tAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTTATCAAAATTTTATCAACAAAAGTTATCAAAACCCCt                                                  >  2:170884/1‑228 (MQ=255)
                                                                                                                                                                    tAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTTATCAAAATTTTATCAAAAAAAGTTATCAAAACCCCt                                                  <  2:39226/228‑1 (MQ=255)
                                                                                                                                                                    tAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGGTATCAAAATTTTATCAAAAAATATTTTCAAAAccccc                                                  >  1:416451/1‑227 (MQ=255)
                                                                                                                                                                    tAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGACTGCTAACGGTGATCAAAACTTTATCAATAAAAGTTATCAAAAACCCt                                                  >  2:320218/1‑228 (MQ=255)
                                                                                                                                                                      aaGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTTATCAAAATTTTATCAAAAAAAGTTATCAAAACCCCTCg                                                >  2:424841/1‑228 (MQ=255)
                                                                                                                                                                       tggTTTGGGAGGCGGAAGTCACGCTAAAACCGTAACCTAGGGCTTCGAGTGTCTGGTGGGTGGCGCTAACCTGCAGAAATGTTCAGAGTTTGGTGACGCCATCAGCGAAAACATGTAATGTCTTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTTATCAAAATTTTATCAAAAAAAGTTATCAAAACCCCTCgg                                               <  1:288271/227‑1 (MQ=255)
                                                                                                                                                                            tGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAACCATGTAATGCCGTAGTTTGTTAAATTTATTAACGGTAGCGTACCGCTCCCGTTGTTTTTTGTTAGGATGCTTACGGTTATCAGAATTTTTTCAAAAAAATTTATCAAAAACCCTCgggtgtt                                          >  1:343871/1‑223 (MQ=255)
                                                                                                                                                                            tGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGATCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTGTTTTTTGGTAGGGCTGCTAACGGTGATCAAAATTTTATCTAAAAAAGTTATCAAACACACTCGTTAg                                            >  2:306395/1‑226 (MQ=255)
                                                                                                                                                                            tGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGGGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGATTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTccag                                                                                                                  >  2:305950/1‑155 (MQ=255)
                                                                                                                                                                             ggAACGCGCAGTCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCgt                                                                                                                 <  1:305950/157‑1 (MQ=255)
                                                                                                                                                                                   cgcAATCAACGCGAAAACCGTAACCTATGACTTCGGGTGTCTGATGGATGACGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACCCGATCATCGAAAACATGTAATGACGTAGTTTGTTAAATTTGTTAACGAGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACTGTTATCAAAATTTTTTATAAAAAATTTGTAAAAACCCCTCTGGAGTTTGTGGGt                                   >  1:697991/1‑228 (MQ=255)
                                                                                                                                                                                   cgcAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTTATCAAAATTTTTTCCAAAAAAGTTATCa                                                          >  1:5545/1‑205 (MQ=255)
                                                                                                                                                                                   cgcAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTTATCAAAATTTTATCAAAAAAAGTTATCAAAACCCCTCGGTGGTTTTGGGGt                                   >  2:232968/1‑228 (MQ=255)
                                                                                                                                                                                   cgcAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTTATCAAAATTTTATCAAAAAAAGTTATCAAAACCCCTCGGTAGTTTTGGGGt                                   >  2:69216/1‑228 (MQ=255)
                                                                                                                                                                                    gcAATCAACGCGAAAACCGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTTATCAAAATTTTATCAAAAAAAGTTATCaa                                                         <  2:5545/205‑1 (MQ=255)
                                                                                                                                                                                               aaaaaCCGTAACCTTTTGCTTCGGGTTGTCTATGGATGGCGCTAAACTGCGTAAATGTTCAGAGTTTGGTGTCGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTTATCAAAATTTTATCAAAAAAAGTTATCAAAACCCCTCGGTAGTTTTGGGGTAGGCTGGCCGGt                       <  1:28427/227‑1 (MQ=255)
                                                                                                                                                                                                    ccGTAACCTATGACTTCGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGGTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTTATCAAAATTTTATCAAAAAAAGTTATCAAAACCCCTCGGTAGTTTTGGGGTAGGCTGGCCGGTCAggt                  <  2:182604/228‑1 (MQ=255)
                                                                                                                                                                                                      gTAACCTATGAATTCGAGGGTCTGATGGTTGGCGCAAAACTTCTTAAATGTTCAGAGTTTGGTTACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTTATCAAAATTTTATCAAAAAAAGTTATCAAAACCCCTCGGTAGTTTTGGGGTAGGCTGGCCGGTCAggtgg                <  1:639204/228‑1 (MQ=255)
                                                                                                                                                                                                                    cGAGTGTCTTAGGGAAGGCGGTAAACTGCTGAAATGTTCAGAGTATGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTTATCAAAATTTTATCAAAAAAAGTTATCAAAACCCCTCGGTAGTTTTGGGGTAGGCTGGCCGGTCAGGTGGTAGTTctactacta  <  2:443784/228‑1 (MQ=255)
                                                                                                                                                                                                                    cGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGACAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGTTGCTAACGGTTATCAAAATTTTATCCACAAAAGTTTTTAAAACCCCCCGGAATTTTTGGGGTAGGCTGGCAGGGCGAGTGGTGGTTcatctaata  >  1:490094/1‑220 (MQ=255)
                                                                                                                                                                                                                    cGAGTGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTTATCAAAATTTTATCAAAAAAAGTTATCAAAACCCCTCGGTAGTTTTGGGGTAGGCTGGCCGGTCAGGTGGTAGTTctactacta  >  2:100362/1‑228 (MQ=255)
                                                                                                                                                                                                                         |                                                                                                                                                                                                                                
GCCCCTGGTGCAAACATCGGTGACGAATGCGCCCTGTTTGAAGCCACCCACGGTACTGCGCCGAAATATGCCGGTCAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGACGCGATCATCGAAAACATGTAATGCCGTAGTTTGTTAAATTTATTAACGGGAGCGTAACGCTCCCGTTGTTTTTTGTTAGGCTGCTAACGGTTATCAAAATTTTATCAAAAAAAGTTATCAAAACCCCTCGGTAGTTTTGGGGTAGGCTGGCCGGTCAGGTGGTAGTTCTACTACTA  >  NC_000913/1196089‑1196528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: