| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,400,230 | T→C | 16.6% | R3G (AGA→GGA) | ogt ← | O‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,400,230 | 0 | T | C | 16.6% | 117.8 / 2.8 | 65 | R3G (AGA→GGA) | ogt | O‑6‑alkylguanine‑DNA:cysteine‑protein methyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (25/27); new base C (7/5); total (32/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.50e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
ATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAAT‑TTTTTCTTCAAGTAA‑TCTCAGCATCCGT‑TCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGG > NC_000913/1400010‑1400447 | atGCAAAGATTACCGGGAAAATATTACCGAAGGTTGTCCTTTAAACCGCCTGGATTGGTTGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTCCAGCAGCTGGACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAAT‑TTTTTCTTCAAGTAA‑TCTCAGCAt < 2:222069/227‑1 (MQ=255) cTAAGATTACCGGCAAAATATTCACGAAACTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTCCAGCAGCTGCACCATGCGTTCCCTGTACTCTTCCCATTCAACCGCCCGCCGGCGAAATTGCTCATCGCCAATCACCCACAGTGGACCCAGTGGCGTGGCAAT‑TTTTTCTTCAAGAAA‑CCCCCGCCTCCGt > 1:408646/1‑228 (MQ=255) aGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAAT‑TTTTTCTTTAAGTAA‑ATCCAGCATCCGt‑ttt > 2:73953/1‑226 (MQ=255) aTTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCCGGCGAAATTGCTCATCGCCAATCACCCACAGTGGCCCCAGTGGCGTGGCAAT‑TTTTTCTTCAATAAA‑ACCCACCATCCGc‑tctct > 1:137324/1‑228 (MQ=255) aTTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGCAATTGCTCATCGCAACTCACTCACAGTGGACCCAGTGGCGTGGCAAC‑TTTGTCTTTAAGAAA‑TCTCACCATCCCt‑ttcc > 2:404377/1‑224 (MQ=255) aTTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAAT‑TTTTTTTTCAAGTAA‑tcttcgaatccctttcct > 2:393636/1‑213 (MQ=255) aTTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGGGGACCCAGTGGCCGGGCCAT‑TTTTTTTTAAAGTAACTCCCAACACCCGT‑tctc > 1:629089/1‑228 (MQ=255) ttcgggCAAAATTTTCCCGAATTTGTTCGTTTAACCCGCCTGGATTGGGGGCAGAAATGCGCTCATAGCCTTCTTTGCCATAATGTATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAAT‑TTTTTCTTCAAGTAA‑TCTCAGCATCCGT‑Tctctct < 1:393636/224‑1 (MQ=255) aCCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCCCAGTGGACCCAGTGGCGGGGCAAT‑TTTTTCTTCCAGTAA‑TCTCACCATcccgttcctctt > 2:632979/1‑218 (MQ=255) ccGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAATGTTTTTCTTC‑AGTAA‑TTCCAGCACCCCT‑TGCCTCttc > 2:251931/1‑227 (MQ=255) aaaaTATTCACGAAGTTTTTCGTTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAAT‑TTTTTCTTCAAGTAA‑TCTCAGCATCCGT‑TCTCTCTTAAGACa < 2:92537/228‑1 (MQ=255) tatTCCCGAAGGTTAGCGCTTAAACCGCCTTGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTTCGATAATGGATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAAT‑TTTTTCTTCAAGTAA‑TCTCAGCATCCGT‑TCTCTCTTAAGACAAACg < 2:435284/228‑1 (MQ=255) aCGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAAT‑TTTTTCTTCAAGTAA‑ATTCAGCAACCGT‑CTTTCCTTAAGCAAAACGTgcg > 2:157026/1‑225 (MQ=255) tccGCTAAACCCGCCTGGATTGGTGGCAGAAATGCCCTCATAGCCTTCTTTGCGATAATGGATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAAT‑TTTTTCTTCAAGTAA‑TCTCAGCATCCGT‑TCTCTCTTAAGACAAACGTGGGTAAATACCCg < 2:137324/226‑1 (MQ=255) ttAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGACAT‑TTTTTTTTTCAGGAA‑TCTCCGCCTCCGG‑TCTCTCTTTAGACACACGTGGGTAAATAccccgtcc > 2:136301/1‑222 (MQ=255) ccTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAATTCACCCACAGTGGACCCAGTGGCGTGGAAAT‑TTTTTTTTCAAGTAA‑TCTCAGCATCCCT‑CCACCCTTAAAACAACCGTGGGCAAATACCCCGAccatccgtgag > 1:73948/1‑219 (MQ=255) tGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAAT‑TTTTTCTTCAAGTAA‑TCTCAGCATCCGT‑TCTCCCTTAAGACCAACGTGGGTAAATACCCGCACATATCgggaca > 1:419121/1‑223 (MQ=255) aTTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCCGTGGCGTGGCAAT‑TTTTTCCTTAAGTAA‑ACTCACCACCCCT‑TCTCCCTTCAGCAAACCCTGGGTAAATAACCGGACCCACCGCCAAAccc > 1:9433/1‑226 (MQ=255) aTTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAAT‑TTTTTCTTCAAGTAA‑TCTCAGCATCCGT‑TCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGa < 2:398716/228‑1 (MQ=255) ggtggCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAAT‑TTTTTCTTCAAGTAA‑TCTCAGCATCCGt‑t > 2:383899/1‑176 (MQ=255) gtggCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAAT‑TTTTTCTTCAAGTAA‑TCTCAGCATCCGT‑tc < 1:383899/176‑1 (MQ=255) tggCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCCCAGTGGCCCCAGTGGCGTGGCAAT‑TTTTTCTTCAAGGAA‑TCCCCGCATCCGT‑TCTCTCTTTAGACAAACGTGGGGTAATCCCCGAGCCAAACGGCAGAccccacaac > 1:609830/1‑221 (MQ=255) ccgAAATGCCCTCATAGCCCTCTTTGCGATACAGGCTGCCCAGCAGCTGCCCCATCCGTTCCCTGTACTCTCCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCTTGGCAAT‑TTTTTCTTCAAGTAA‑TCTCAGCATCCGT‑TCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATAc < 1:157026/226‑1 (MQ=255) aTGCGCTCATAGCCTTTGTTGCGATAATGGATGTCCAGCAGCTGCCCCATGCGTTCGCTGGACTCTTCCCATACAACCGCCCGGAGGCGAAATTGCTCATCGCAAATCGTCCACAGTGGACCCAGTGGCGTGGCAAT‑TTTTTCTTCAAGTAA‑TCTCAGCATCCGT‑TCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACg < 2:394208/227‑1 (MQ=255) aTAGCCTTCTTTGCGATAATGGATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAAT‑TTTTTCTTCAAGTAA‑TCTCAGCATCCGT‑TCTCTCTTAAGACAAACCTTGGTAAATACCCGGACCTATCGGCAGACCGCCACGATACCCACCCCGCAACa > 2:60525/1‑228 (MQ=255) aTAGCCTTCTTTGCGATAATGGATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAAT‑TTTTTCTTCAAGTAA‑ACTTAGCATCCGT‑TCTCTCTTAAGACAAACATGGGTGAATACCCGGACCTATCTGCAGACCGACAAGATACCACGCCAGCAACa > 2:489102/1‑228 (MQ=255) tcccTTTTTTGCGATAATGGATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAAT‑TTTTTCTTCAAGTAA‑TCTCAGCATCCGT‑TCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATc < 1:632979/226‑1 (MQ=255) cttTGCGATAATGGATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAAT‑TTTTTTTTCAAGTAA‑TCTCAGCATCCGT‑TCTCTCTTAAGACAAACGGGGGTAAAAACCCGGACCCATCGGCCGACCGACAAGATACCACGCCAGCAACATCagcagc > 2:552114/1‑228 (MQ=255) cttTGCGATAATGGATGCCCAGCAGCTGAACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGGGGACAT‑TTTTTCTTCAAGGAC‑TCTTATGATCCGTATTGCTCTAATGA‑AAACGTGGTGGAAAACCCGGACCATACGGCAGAACCTCAAGACAACCCGCCGCCAAAAAAagcagc > 1:500812/1‑228 (MQ=255) cGATAATGGATGTCCAGCAGCTGCACCATGAGTTCGCGGTACTCTTCCAATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAAT‑TTTTTCTTCAAGTAA‑TCTCAGCATCCGT‑TCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATac < 2:408646/228‑1 (MQ=255) aagggATGCCCACCCGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCGACCCCCCGCAGGCGAAATTGCTCATCGCAAATCCCCCACAGTGGACCCAGTGGCGTGGCAAT‑TTTTTCTTCAAGTAA‑TCTCAGCATCCGT‑TCTCTCTTAAGACAAACGTGGGTAAATCCCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATaccacc < 1:552114/225‑1 (MQ=255) ggATGCCCAGCCACTGCACCATGCGTTCGCTTTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCCCCCACAGTGGACCCAGTGGCGTGGCAAT‑TTTTTCTTCAAGTAA‑TCTCAGCATCCGT‑TCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCaaa < 2:141914/228‑1 (MQ=255) ggATGCCCAGCACCTTCCCCATGCGTTCGATGTCCTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCCCCCACAGTGGACCCAGTGGCGTGGCAAT‑TTTTTCTTCAAGTAA‑TCTCAGCATCCGT‑TCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCaaa < 2:609830/228‑1 (MQ=255) gATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAAT‑TTTTTCTTCAAGTAA‑TCTCCGCATCCGT‑TTTCTCTTAAAACAAACGTGGGGAAATACCCGGACCTATCGGCATGCCGACCAAATACAACGCCAGCAACATCAGCAGCACTACCACCaaaa > 2:354633/1‑228 (MQ=255) gctgccctatcgcttCGCTTTCCCCTCCCCATTAAACCGCCCGCAGGCTAAATTGCTCATCGCAAATCCCCCACAGTGGACCCAGTGGCGTGGCAAT‑TTTTTCTTCAAGTAA‑TCTCAGCATCCGT‑TCTCTCTAAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGa < 1:242540/215‑1 (MQ=255) gcTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAAT‑TTTTTCTTCAAGTAA‑TCTCAGCATCCGT‑TCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGCCCAGATACCACGCCAGCAACATCAGCAGCCATaccactgtctcttctacaaa > 2:349470/1‑212 (MQ=255) atctgtccGCTGCACTCTTCCCAATCAACCGCCCGCCGGCGAAATTGGTCATCGCAAATCACCCCCAGGTGACCCTGTGGCGTGGCAAT‑TTTGTCGTGAAGTAA‑CCCCAGCATCCGT‑TCACTCTTAAAAATAACGGGGGTAAATACCCGGACCTAACGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCaa < 1:621086/221‑1 (MQ=255) tGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAAT‑TTTTTCTTCAAGTAA‑TCTCAGCATCCGT‑TCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAg > 2:242540/1‑228 (MQ=255) tGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAAT‑TTTTTCTTCAAGTAA‑TCTCAGCATCCGT‑TCTCTCTTAAGACAAACGCGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGACAGCAACATCAGCCACCATAACACCAAAAAGATAAGTGGACAgggaaca > 2:302854/1‑224 (MQ=255) tGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAAT‑TTTTTCTTCAAGTAA‑TCTCAGCATCCGC‑TCTCTCTTAAGACAAACGTGGGTAAATACCCGAGCCTATCGCACAACCGAAAAAATACCACGCCCACAAAATCCACACACACACCACCCAAAAAATaaaaggaaaaggccaa > 1:447457/1‑214 (MQ=255) ttgccTGTACTCCTCCCTATCACCCGCCCCCAGGTGAAATTTCTAAGCTAAAATCACCCACAGCGTACCCAGTGGCGTGTCAAT‑TTTTTCCTCAAGTAA‑CCGCAGAATCCGT‑TCGCTCATAAGACAAACGGGGGTAAATACCCGGCCCTATCGGCAGACCGACAAGATGCCACTCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGa < 1:489102/221‑1 (MQ=255) tCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAAT‑TTTTTCTTCAAGTAA‑TCCCAGCCTCCGT‑TCTCTCTTAAGACTAACGTGGGTAAAAACACGGACCTATCGTCACACCGTCAAGTTACAACGCCAGCAACATCAGCAGCCATCCCCCCCAAAAGATAAGAGGATAGGGCAAGACtca > 2:129853/1‑226 (MQ=255) ctttccctctCCCATTCAACCCCCCCCAGGCGAAATTTCTTCACACAAATCACCCCCAGTTGACCCAGTTGCGTGGCAAT‑TTTTGCTTCAAGTAC‑TCCCAGCATCCGT‑TCGCCCGTAAGACAACCGTGGGTAAATACCCGGACCCATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATTAGTGGATAGGGCAAGACTAACGa < 1:628493/219‑1 (MQ=255) ccctcccccTTTAACCCCCCGGAGGCGAAATTGCTCTTCGCAAATCCCCCACAGTGGCCCCAGTGGCGTGGCAAT‑TTTTTCTTCAAGTAA‑TCCCAGCATCCGT‑TCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATagt < 1:60525/223‑1 (MQ=255) ttCCCATTCAACCGCCCGCAGGCGAACTTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCCAT‑TTTTTCTTCAAGTAA‑TCTCAGCATCCGT‑tctccc > 1:658006/1‑104 (MQ=255) tCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAAT‑TTTTTCTTCAAGTAA‑TCTCAGCATCCGT‑Tctctct < 2:658006/106‑1 (MQ=255) ccATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAAT‑TTTTTCTTCAAGTAA‑TCTCAGCATCAGT‑GCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATCCGCCGACCGACAAGATACCACGCCAGCAACATCAGCAGCCACTCCAACAAAAAGATAAGTGGAAAGGGCAAGACTAACGAAAAGTAAATAc > 2:688586/1‑228 (MQ=255) tCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAAT‑TTTTTCTTCAAGTAA‑TTTCAGCATCCGT‑TCTCTCTTAAGAc > 2:340193/1‑106 (MQ=255) cAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAAT‑TTTTTCTTCAAGTAA‑TCTCAGCAGCCGT‑TCTCTCTTAAGACa < 1:340193/106‑1 (MQ=255) aCCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAAT‑TTTTTCTTCAAGTAA‑TCTCAGCATCCGT‑TCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTgg > 2:87112/1‑192 (MQ=255) ccgcccgcAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAAT‑TTTTTCGTCAAGTAA‑TCTCAGCATCCGT‑TCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGa < 1:87112/192‑1 (MQ=255) gcccgcAGGCTAAATTGCTCATCGAAAATCACCCACAGTGGACCCAGTGGCGTGGCAAT‑TTTTTCTTCAAGTAA‑TCTCAGCATCCGT‑TCCCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTcgc < 2:186276/228‑1 (MQ=255) ccgcAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAAT‑TTTTTCTTCAAGTAA‑TCTCAGCATCCGT‑TCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGt < 2:9433/228‑1 (MQ=255) ccgcAGGCGAAATTGCTCATCGCAAATCACCCACAGTGCACCCAGTGGCGTGGCAAT‑TTTTTCTTCAAGTAA‑TCTCAGCATCCGT‑TCTCTCTGAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGt < 2:419121/228‑1 (MQ=255) cgaaGGGGAAATTGCATATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAAT‑TTTTTCTTCAAGTAA‑TCTCAGCATCCGT‑TCTCTCTTAAGACAAACGGGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTc < 2:665140/225‑1 (MQ=255) cccgtggcgtggcAAT‑TTTTTCTAAAAGTAA‑TCTCAGCCTCCGT‑TCTCGCTAAAAAAAAACGTGGGGAAAGACCCGGCCCTACCGGCAAACCAACAAGATGCCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACa < 1:136301/225‑1 (MQ=255) aGTGGCGTGGCAAT‑TTTTTCTTCAAGTAA‑TCTCAGCATCCGT‑TCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCCCGCCAGCAACATCAGCAGCCCTACCACCAAAAACATAAGTGGATAGGGCAAGACTAACGCAAAGTAAATACCCCCGTTCCCAGCTGGTTTGTAACGTTGCAGGAAACCAAAAAACAGAGGaaaaaa > 1:143680/1‑228 (MQ=255) gtggcgtggcAAT‑TTTTTCTTCAAGTAA‑TCTCAGCATCCGT‑TCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAAGGCAAGACTAACGAACAGTAAGTACCCAGTTTCGCGGCTGGTTTGTAGGGTTTCGAGAAAACAAAAAAAAGAGGGACaaaa > 1:527879/1‑228 (MQ=255) gtggcgtggcAAT‑TTTTTCTTCAAGTAA‑TCTCAGCATCCGT‑TCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAAAATCAACAGCCATACCACCAAAACAAAAAGTGGAAAGGGCAAGAATAACGAATAAAAAATGCCCAAGTTCCCGTCCGGTGTGTAGCGGTGCAGGAATCCAAAAAAAAAGGGAaaaaaa > 1:358876/1‑228 (MQ=255) gcgtggcAAT‑TTTTTCTTCAAGTAA‑TCCCAGCATCCGT‑TCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGt < 2:305975/181‑1 (MQ=255) gcAAT‑TTTTTCTTCAAGTAA‑TCTCAGCATCCGT‑TCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATAc < 2:629089/228‑1 (MQ=255) gtcttcAAGTAA‑CCTCCGCCTCCCT‑CCCCCCGAAAAACAAACGTGGATAAAGCCCCGGACCTACCGGCAAACCGAAAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCt < 1:548249/226‑1 (MQ=255) ttttaaaGTAA‑GCCCACCATCCGT‑TCTCTCTTAAGACAACCGGGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTaa < 1:395859/223‑1 (MQ=255) cttcAAGTAA‑TCTCAGCATCCGT‑TCTCCCTTAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTaaa < 2:527879/228‑1 (MQ=255) ttcAAGTAA‑TCTCAGCATCCGT‑TCTCTCTTAAGACAAACGTGGGTAAATACCCGTACCTATCGGCAGACCGACAAGATCCCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTaaa < 2:462813/227‑1 (MQ=255) tcAAGTAA‑TCTCAGCATCCGT‑TCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGATTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACATTGGAACAAAAGGAGAAACCGGCGACAAAgg > 2:295604/1‑228 (MQ=255) | ATGCTAAGATTACCGGCAAAATATTCACGAAGCTTGTCGCTTAAACCGCCTGGATTGGTGGCAGAAATGCGCTCATAGCCTTCTTTGCGATAATGGATGTCCAGCAGCTGCACCATGCGTTCGCTGTACTCTTCCCATTCAACCGCCCGCAGGCGAAATTGCTCATCGCAAATCACCCACAGTGGACCCAGTGGCGTGGCAAT‑TTTTTCTTCAAGTAA‑TCTCAGCATCCGT‑TCTCTCTTAAGACAAACGTGGGTAAATACCCGGACCTATCGGCAGACCGACAAGATACCACGCCAGCAACATCAGCAGCCATACCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGG > NC_000913/1400010‑1400447 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |