Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,132,7870AC100.0% 186.9 / ‑6.7 64I204S (ATC→AGC) wcaAputative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (2/0);  new base C (28/33);  total (31/33)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CCCGAATACCATCAGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGCCGGTTGGGA  >  NC_000913/2132566‑2133004
                                                                                                                                                                                                                             |                                                                                                                                                                                                                         
cccGAATACCATCAGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGc                                                                                                                                                                                                                      >  2:524721/1‑227 (MQ=255)
  cgcttacccgcggCCAGGCGTTTGCCGTTACGGTGCGAGAGCGGGGACGGGAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTTGAGGGTAAACAGGTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTCCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTc                                                                                                                                                                                                                   <  1:356316/216‑1 (MQ=255)
          aTCAGCCAGACGTTTGCCTTTTCGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATAGGTTTATTGCGGATCTGGTAGAGGGTAAACAGATGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtctt                                                                                                                                                                                                            <  2:216179/227‑1 (MQ=255)
          aTCAGCCAGACGTTTGCCGTTACGCACCGGCAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtctt                                                                                                                                                                                                            <  2:41345/227‑1 (MQ=255)
           tCAGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtcttct                                                                                                                                                                                                          >  2:20033/1‑228 (MQ=255)
           tCAGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCtcttct                                                                                                                                                                                                          >  2:348522/1‑228 (MQ=255)
                  gACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGGAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGTTCTGCGTCGCATTTTCTACTTTcc                                                                                                                                                                                                   >  1:447713/1‑228 (MQ=255)
                   aCGTTTGCCTTTCCGCACCGACAGCAGCGTGAGCAAAGTGCGCCAGGTCATACGTTTATTGGGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTTATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa                                                                                                                                                                                                  <  2:133056/228‑1 (MQ=255)
                   aCGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGACTTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGTGGGTATCTGCATTTCACCGTGATTGATAGGCAGGCTCTGGGTCGCCTCTTCTACTTTCCa                                                                                                                                                                                                  >  2:701305/1‑228 (MQ=255)
                       ttGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGCCGCCCCTTCTACTTTCCACgg                                                                                                                                                                                               >  2:505432/1‑227 (MQ=255)
                         gCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGACTGGCGGGGCCCCTTTTACTTTCCACgggt                                                                                                                                                                                             >  1:459865/1‑225 (MQ=255)
                             tttttaccgggcagcggCGTGAGCAAAGTGGCCTAGGTCATTCTTTTATGGGGGGTTTGGTAGAGGGTAAGCAGCTGATATTGTTGGCTGGCGCGGGCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTCCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg                                                                                                                                                                                        <  1:227656/217‑1 (MQ=255)
                             ttcccccccGACAGCGGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg                                                                                                                                                                                        <  2:459865/221‑1 (MQ=255)
                             ttccGCTCCGACAGCAGGGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg                                                                                                                                                                                        <  2:568475/225‑1 (MQ=255)
                             ttACGGACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCg                                                                                                                                                                                        <  2:512911/228‑1 (MQ=255)
                             ttACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGGGAATTTTTTCGGTGACTAGGTGATCTGCATTTCACCGTGTTTGATCTGCAGGCTCTGCGTCGCCTCTTCTCCTTTCCACGGTTCGcc                                                                                                                                                                                         >  2:702937/1‑227 (MQ=255)
                                    acgaaatcggcGTAAGCAAGGTGCGCCAGGTCATGCGTTTATTGCGGGTCTGGTAGAGGGTAAAAAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTGCCCGGAGAATTTTTTCGGTGCCGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTcca                                                                                                                                                                                 <  2:297558/219‑1 (MQ=255)
                                       aCAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTccacca                                                                                                                                                                              >  2:205796/1‑228 (MQ=255)
                                            agggacAGCAAAGTGGTCCTGGTCTATCGTTTATTGGGGTTTTTGTAGAGGTTAAACAGCTGATAGTTTTTGCTGGTGCGGTAGAATTTGTCTTTGTGTTTGCGGTAAAAGTGGACGTCCCCGGAGAATTTTTTCGGTTACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACGCCACCACCATc                                                                                                                                                                         <  1:702937/223‑1 (MQ=255)
                                              cGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATcc                                                                                                                                                                        >  2:15430/1‑227 (MQ=255)
                                                tCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGGGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCACCCCCa                                                                                                                                                                     >  2:567879/1‑228 (MQ=255)
                                                  aGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATTCAGGCTTTGCGTCGCCTCTTCTACTTTCCACGGTTCCCCGTACTCCACCACCACCCGCAgg                                                                                                                                                                   >  2:108087/1‑228 (MQ=255)
                                                   gCAAAGTGCACCAGGTCATGCGTTTAATGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCTCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAgg                                                                                                                                                                   <  2:126688/227‑1 (MQ=255)
                                                       atggcgcCAGGTCATACGTGTTTTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGa                                                                                                                                                               <  2:539099/224‑1 (MQ=255)
                                                       agggcgcCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGa                                                                                                                                                               <  2:243878/224‑1 (MQ=255)
                                                       aGTGCGCCAGTTTATACGTTTATTGCGGATGCGGTAGAGGGTAAACAGCTTATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTTCTTGCGGTAAAAGTGGAAGTTCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCTGCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGa                                                                                                                                                               <  2:292041/227‑1 (MQ=255)
                                                       aGTGCGCCAGGTCATACGTTTATTGCGGATCTTGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGa                                                                                                                                                               <  2:447713/227‑1 (MQ=255)
                                                         tggtccAGGTTATACGTTTGTTGCGTATCTGGTTGAGGGTAAACATCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTGCCCGGAGAATTTGTTCGGTGACGAGGTGATCTCCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATg                                                                                                                                                             <  1:478005/223‑1 (MQ=255)
                                                              cAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGGGATCTGCATTTCACCGGGATTGATATGCAGGCTCTGCGTAGCCTCTTCTACTTTCCACGGTTAGCCGAAATCCACCACCCTCCGCAGGAAGATGCCGt                                                                                                                                                         >  2:676408/1‑226 (MQ=255)
                                                                     gaggTTTATGTCGGATCTGGTTGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTTTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgtg                                                                                                                                                <  2:574624/225‑1 (MQ=255)
                                                                           tATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTCCCGCt                                                                                                                                           >  2:140993/1‑227 (MQ=255)
                                                                                  gATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGACTTTGTCTTTGTGCTTGCGGTAAAAGCGGAATTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACGGTGATTGATATGCAGGCTCTGCGTTGCCTCTTCTACTTTCAACGGTTCGCCGTACTCCACCCCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTc                                                                                                                                   >  2:17213/1‑228 (MQ=255)
                                                                                  gATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTTGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATCTGCATGCTCTGCGACGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGCTGTCGTAATCCTGTTCACCTTTCAGTTc                                                                                                                                   >  1:695065/1‑228 (MQ=255)
                                                                                       tgtggggggTTAACCGCTTGTTTTTTTTGCTGGGGCGGTCGAATTTGTCTTTGTGCGTGCGGTAAAAGTGGAAGTTCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCCCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTAt                                                                                                                              <  1:20033/222‑1 (MQ=255)
                                                                                             gggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGCGGTGATCTGCATTTCACCGTGATTGATATGCAGGAGCGTCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACACCCATCCGCCGGAAGCGTTCGGAATCTGTTGCCGCTTTCAGTCCGGGATCGacc                                                                                                                         >  1:516239/1‑225 (MQ=255)
                                                                                              gttAAACAGGTGATTTTTTTTGGTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaaca                                                                                                                        <  1:140993/225‑1 (MQ=255)
                                                                                              ggTAAACAGGTGATATTTTGTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTAt                                                                                                                              <  2:516239/221‑1 (MQ=255)
                                                                                              ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCCCCACCACCCCCAGGAAGATGTCGTAATCCTGTGCCCCTTTCAGTTCGGTATCGaacaa                                                                                                                       >  2:380214/1‑228 (MQ=255)
                                                                                              ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCCCCATCCCCCGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCCGTATCGaacaa                                                                                                                       >  1:465615/1‑228 (MQ=255)
                                                                                              ggTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACAGTGATTGATATGCAGGCTCTGCGTCGCCGCTTCTACTTTCCACGGTTCGCCGGACTCCACCACCCTCGGCGGGAAGATGTCGTATTCCTTGGCGCCTTTCAGTTCGGCATCGaacaa                                                                                                                       >  1:604289/1‑228 (MQ=255)
                                                                                                 aaaaAGGTGATTTTTTTTTGGGGCGGGGTGGAATTTGTTTTTGTGCGTGGGGTAAAAGTGGAAGTACCGGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaacaaaca                                                                                                                    <  1:380214/228‑1 (MQ=255)
                                                                                                       cTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGCTGTCGTCATCCTGTGCCGCTTTCAGTTCGGCATCTAACAAACCCTCtt                                                                                                               >  2:3658/1‑227 (MQ=255)
                                                                                                        tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCttt                                                                                                              >  2:281264/1‑227 (MQ=255)
                                                                                                           tatTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGaa                                                                                                           <  2:604289/227‑1 (MQ=255)
                                                                                                           tatTTTTTGCGGGGGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAACGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGaa                                                                                                           <  2:434523/227‑1 (MQ=255)
                                                                                                             ttttttGCGGGGGCGGGCGAATTTGTTGTTGGGGCTGCGGTAAAAGGGGAAGTACCCGGAGAATTTTTGCGGTGACGAGGTGATCTGCATTTCCCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACg                                                                                                        <  1:15430/228‑1 (MQ=255)
                                                                                                               ttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCCCCGGAAGATGTTGTAATCCTGTGCCGTTTTCAGTTCGGTCTCGAACAAAAACCCTTTGAAACGcc                                                                                                      >  1:379335/1‑228 (MQ=255)
                                                                                                                    cTGGCGCGGTCGAATTTTGTTTTTTGCTTGCGGTAAAAGTGGAAGTCTCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCCCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGcc                                                                                                 <  1:205796/228‑1 (MQ=255)
                                                                                                                     tGGCGGGGTCGATTTTTTTTTTGGGCTTGCGGTAAAAGTTGAAGTTCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCCCCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGccc                                                                                                <  1:359052/228‑1 (MQ=255)
                                                                                                                     tGGCGCGGTCTAATTTGTTTGTGTGTTTGCGGTAAAATGTGAAGTCGCCGGAGAATTTTTTCGTTGACGAGGGGATCTGCATTTCCCCGGGATTGATATGCACGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGGCGTACTCCACCGCCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGcc                                                                                                 <  1:108087/227‑1 (MQ=255)
                                                                                                                         gcgtgtgAATTTGTTTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTTATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGt                                                                                            <  2:99932/222‑1 (MQ=255)
                                                                                                                         gcgGTCGAATTTGGCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCCCCGTGCTTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGAACTCCACCACCATCCGCAGGAAg                                                                                                                                                                >  2:95802/1‑160 (MQ=255)
                                                                                                                             tCGAATTTGTTGTTGTGCTTGCGGTAAAAGTGGAAGGCCCCGGAGAATTTTTTCGGTGACGAGGTGACCTGCATGTCCCCGGGATTGATATGCAGGCTCTGCGTCGCCTCTGCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTGTCAGTTCGGTATCGAACAAACTCTCTTTGAAACGCCATGCCCAGGTAAAg                                                                                        <  1:140272/228‑1 (MQ=255)
                                                                                                                                                             gAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGaa                                                        >  1:191922/1‑228 (MQ=255)
                                                                                                                                                               aGTACCTGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCCTTGAAACGCCATGCCCCGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACa                                                      >  2:421221/1‑228 (MQ=255)
                                                                                                                                                                                                  tGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTTCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGCCCTGGTTg                                                                               >  1:466933/1‑168 (MQ=255)
                                                                                                                                                                                                   gCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGc                                                                              <  2:466933/168‑1 (MQ=255)
                                                                                                                                                                                                        tCACCTGGATTGGTATGCAGGCTTTGCGGCGCCTCTGCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCt              <  2:443997/227‑1 (MQ=255)
                                                                                                                                                                                                            cGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGCACACACACTCTTTGAAACGCCATGCCCAGGTAACGACCTGGTTGCCAATGATATTGCGTTTGTAGAACACGCGGCGTGAAAACGGCGATTTGGGATACAGCGGCAAACTTGcc          >  2:226028/1‑227 (MQ=255)
                                                                                                                                                                                                            cGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGc                                                                                                  >  1:90401/1‑139 (MQ=255)
                                                                                                                                                                                                             gTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGcc                                                                                                 <  2:90401/139‑1 (MQ=255)
                                                                                                                                                                                                              tgattgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAAAACTCTTTGAAACGCCATGCCCAGGCACAGACCTGGTTGCCAATGATATTGCTTTTGCAGAACAGGCGGCGGGACAACGGCGCTTTgggcacaagcgccagaatttccg         >  1:703372/1‑206 (MQ=255)
                                                                                                                                                                                                              gttttgatATGCAGGCTTGGCGTCGCCCTTTCTACTTTCCACGGTTCGCCGTCCTCCACCCCCATCCGCAGGAAGATGTCGTAATCCTGGGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGCCGGt       <  1:348522/225‑1 (MQ=255)
                                                                                                                                                                                                                    atatGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACAATCTTTGAAACGCCATGCCCAGGTATAGACCTGGTTTCCACTGCTATTGCGTTTGTAGACCAGGCGGCGTGAATCACGCGCTCTGGGATAACGGGGCAACCATGCCGGGTGGGa  >  1:295055/1‑227 (MQ=255)
                                                                                                                                                                                                                             |                                                                                                                                                                                                                         
CCCGAATACCATCAGCCAGACGTTTGCCGTTACGCACCGACAGCAGCGTCAGCAAAGTGCGCCAGGTCATACGTTTATTGCGGATCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTTGCCAATGATATTGCGTTTGTAGAACAGGCGGCGTGAATACGGCGATTTGGGATACAGCGGCAAGCTTGCCGGTTGGGA  >  NC_000913/2132566‑2133004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: