| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,419,689 | A→C | 20.7% | V33G (GTG→GGG) | yfcE ← | phosphodiesterase activity on bis‑pNPP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,419,689 | 0 | A | C | 20.7% | 83.2 / 3.6 | 53 | V33G (GTG→GGG) | yfcE | phosphodiesterase activity on bis‑pNPP |
| Reads supporting (aligned to +/- strand): ref base A (24/14); new base C (3/12); total (27/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.28e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCG‑CTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGTTTCATCACTCACTCCTCG‑TCGAAAAAACGTAAGCTATGATACTGGATTCTGTCGTTGCTATCAGCCAGATTGCTTCGCCGAAAGTGCAATAAAACGCTGGACAGACGCTCGCTGCCACTGGAATGTTG > NC_000913/2419475‑2419906 | cGTAAAAAACAGCGGTTGTTTTCCCAGTAATCCCTGTGGCCCCGGCGCGGTGATCGGGAAATGCAGGATCATTGGATCCACTTCGGTGGCGCAGTGGCCGCGCACAGGGATACCCTTATGTGCCACTTCATTAAGCCGTTCAGCGGCTTTGGCTGGCGCGTAACCCCCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGc < 1:516149/228‑1 (MQ=255) gACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTTCCCACGGATCACCACCCACTGGGCCCCG‑CTTTggg > 1:406146/1‑228 (MQ=255) aCGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGGGCACAGCGATAACCTTATGTGCAACTTCATTAAGCCTTTCAGCTCCTTTGGCTGGCGCGTAAACCTCCGGCAACGCATTACGCGGGCCGCGATTCAACACGTCCCCCCGGATCCCCATCCCCTGGG‑ACCGCCTTTGGGc > 1:524529/1‑228 (MQ=255) aCGTTGTTTTTCCAGCAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCAACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATGCAACCCGTCGCCAAGGATCACCCGCCACTGGGCACCG‑CTTTGGGc > 2:579801/1‑228 (MQ=255) ttgttTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTAAACACGTCGCCAAGCGTCCCCAGCCCCCGGGCACCG‑CTTTGGGCaaa > 1:86040/1‑228 (MQ=255) gTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCCGGCCGGGATTCCACACGTCGCCAAGGATCACCACCCACTGGGCCCCG‑CCTTGGGCAAACAACCCCAGaa > 1:520429/1‑228 (MQ=255) tGTTGCCCCGGGGGGGTTCACGGGAATTGCAGCAGCATTTGATCCACTTCGCTGGCGCAGGTGCCGCGCACAGCGATAACCTTAGTTGCCACTTCATTAAGCCGTTCAGCGACGTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCG‑CTTTGGGCAAACAACTCCAGAACACGTTcc < 1:630984/228‑1 (MQ=255) ttGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCTCGTAACCCTCCGGTAAAGCATTACGCAGGCCGTGATTCAACACGTCGCCAAGGAACCCCAGCCACTGGGACACG‑CTTTGGGCAACAAAATCCGGACCACGTCCCg > 2:380111/1‑227 (MQ=255) tGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGTCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCACGGATCACCAGCCACTGGGAACCG‑CTTTGGGCAAACAACTCCAGAACACGTTCCGt > 2:99383/1‑227 (MQ=255) tGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCG‑CTTTGGGCAAACAACTCCAGAACACGTTCCgc > 2:168217/1‑226 (MQ=255) gCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCG‑CTTTGGGCAAACAACTCCAGAACACGTTCCCTc > 2:88612/1‑227 (MQ=255) aCGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCG‑CTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCg > 2:83378/1‑228 (MQ=255) gcgTTTATCGGGAAATGCAGCAGCTTTTGATCCCCTCCGTGTTCCCGGTTGCCGGCCACTGCGATACCCTTATGTGCCACTGTATTAGGCTGTCCAGGGCCTTTGGCTGGCCCGTAACCCCCCGGTAAAGCATTCCGCGGGCCGTGATTCAACCCGTCGCCAAGGATCACCAGCCACTGGGCACCG‑CTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAAc < 1:39204/228‑1 (MQ=255) ggggtgttgggaaattgtgtagcaTTTGTTCCACTTTCCTTTCGCAGTTGCCGGCCCCAGGGTTAACCTTATTGGCCACTTCATTAAGCCTTCTAGCGGCTTTGCGTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGACCCCCAGCCACTGGGCACCG‑CTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACg < 1:599350/208‑1 (MQ=255) gcaTTTGATCCACTTCGCTTTCGCAGTTGCCGCGCAAAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCG‑CTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCa < 2:215216/227‑1 (MQ=255) gATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCG‑CTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAACCATc > 2:89519/1‑227 (MQ=255) tGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCG‑CTTTGGGCAAACAACTCCAGAACACGTTCCGTAGCCGGTAACGGCCCATGAATGTCCGATGCAAACACAAGGTTCAtcactc > 2:498615/1‑228 (MQ=255) tCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCG‑CTTTGGGCAACCAACTCCAGAACACGTCCCGCCCCCGGCACCGGCCCATGACATCCCCACCAAAACAACAGTTTCAccactaac > 1:305993/1‑228 (MQ=255) cgtgctcaGCGGTACCCTGTTGGGCCCCTTCATTGACCCAGTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGGAAAGCCTTTCGCGGGCCGTGATTCAACCCGTCGCCAAGGATCCCCAGCCACTGGGCTCCG‑CTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGTTTCATCACTCACTCCTCG‑TCGa < 1:99383/222‑1 (MQ=255) cgcACAGCGAAAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCG‑CTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGTTTCATCACTCACTCCTCG‑TCGaaa < 2:592634/228‑1 (MQ=255) ggataccttgttgtgCCCTTTCATTAAGCCGTTCGGCGCATTTGGCGGGCGCGTAACCCCCCGGAAAAGCCTTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCCCCAGCCACTGGGCACCG‑CTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGTTTCATCACTCACTCCTCG‑TCGAAAAAACGTa < 1:89519/217‑1 (MQ=255) cGATAACCGTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTACCCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCG‑CTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGTTTCATCACTCACTCCTCG‑TCGAAAAAACGTa < 2:366110/228‑1 (MQ=255) ccACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCG‑CTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGt > 1:384201/1‑182 (MQ=255) cACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCG‑CTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGtt < 2:384201/182‑1 (MQ=255) cACTCCATTAAGCCGTTCAGCGCCTTTGGCTGGCGCGTACCCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCG‑CTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACa < 2:629604/176‑1 (MQ=255) cTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGAATCAACAGCCACTGGGCACCG‑CTTTGGGCAAACAACCCCAGAACACGTTCCGTCCCCGATAACGACCCAGGAATGTCCGATCACAACATCCGTTTCTTCACTCACTCCCCGCACGaaaaacacaaccaaaaatacagaaacc > 1:298695/1‑206 (MQ=255) ttCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGTTTCCAGACGTAGCCACGGATCACCAGCCACTGTGAACCG‑CTTTGGTCAAACAACTCCAAAACACGTTCCGCCGACGTTACCGCCCAATTAGTGTACGATGCAAACCTCAGTTTCATCACTCACTCTACA‑TCGACAAAAAATAAGcaagaaaacaggctca > 1:82385/1‑212 (MQ=255) tCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCG‑CTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGTTTCATCACTCACTCCTCG‑TCGAAAAAACGTAAGCTATGATACTGGATTCTg > 2:487238/1‑228 (MQ=255) ttAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCG‑CTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGCCCGATGCAAACATCAGTTTCCTCACTCACTCCTCG‑CCGCAAAAACGTAAGCTATGATACTGGATTCTGTct > 2:15717/1‑227 (MQ=255) tagccctttccgggcgttgggccggcGCGTACCCCCCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCG‑CTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGTTTCATCACTCACTCCTCG‑TCGAAAAAACGTAAGCTATGATACTGGATTCTGTCGt < 1:393492/209‑1 (MQ=255) tggCCGTTCAGCGCCTGTGGCGCGCGGCACACCCTCCGTGACAGCGTTCGGGGGGCCCTTATTCAACCCGACGCCAAGGATCCCCAGCCACTGGGCACCG‑CTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGTTTCATCACTCACTCCTCG‑TCGAAAAAACGTAAGCTATGATACTGGATTCTGTCGt < 1:524956/225‑1 (MQ=255) aaGCCGTTACGCGACTTGGGCTGGCGCGTAACCCTCGGGTAAAGCGTTACGCGGGACGGGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCG‑ATTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGTTTCATCACTCACTCCTCG‑TCGAAAAAACGTAAGCTATGATACTGGATTCTGTCGt < 2:82882/227‑1 (MQ=255) ttcacggattttgcgcggcGCGTCCCCCCACGGTAAACCATTGCGGGGGCCGTGATTCAACACGCCGCCAAGGATCCCCAGCCACTGGGCACCG‑CTTTGGGCAAACTACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGTTTCATCACTCACTCCTCG‑TCGAAAAAACGTAAGCTATGATACTGGATTCTGTCGTTGCTATc < 1:259818/212‑1 (MQ=255) ttCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCG‑CTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGCTGCAAAAATCAGTTTCATCACTCACTCCTCG‑TCGAAAAAACGAAAGCTATAATACTGGATTCTGTCGTTTCTATc > 1:142454/1‑228 (MQ=255) ttCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCG‑CTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGTTTCATCACTCACTCCTCG‑TCGAAAAAACGTAAGCTATGATACTGGATTCTGTCGTTGCTa > 2:92767/1‑226 (MQ=255) ttCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCG‑CTTTGGGCAAACAACTCCAGAACACCTACCGTCGCCGGACACGACCCATGAATGACCGCTGCAAACCTCATATTCATCCCTCACTCCCCC‑TCGAAAAAACCTAAGCTATGAAACAggctccctccgttcctatc > 1:132717/1‑211 (MQ=255) cgcgTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCC‑CTTTGGGCACACAACCCCAGAACACGTTCCGTCGCCGGTAACGCCCCATGCACGTCCGCTGCAAACATCGGTTTACTCCCTCACTCCTCG‑TCGCAAAACCGCAAGCTCTGTAACTGGTTTCTGTCTTTGCTaccgcccaactagctgctccg > 1:345522/1‑208 (MQ=255) ccccTCCGGTAACGCATTCCGCGGGCCGTAATTCAAACCCCCGCCAAGGATCCCCTGCCACTGGGCCCCG‑CTTTGGTCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGTTTCATCACTCACTCCCCG‑TCGAAAAAACGTAAGCTATGATACTGGATTCTGTCGTTGCTATCAGCCAGATTGCTTCGCCGAAAGTg < 1:206474/227‑1 (MQ=255) cGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCG‑CTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGTTTCATCACTCACTCCTCG‑TCGAAAAAACGTAAGCTATGATACTGGATTCTGTCGTTGCTATCAGCCAGATTGCTTCGCCGAAAGTGCAATa < 2:583906/227‑1 (MQ=255) cATTACGCGGGCCGTGATTCAACACGTCGCCCAGGATCACCAGCCACTGGGCACCG‑CTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAAAGTCCGATGCAAACATCAGTTTCATCACTCACTCCTCG‑TCGAAAAAACGTa > 1:300288/1‑159 (MQ=255) cATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCG‑CTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGTTTCATCACTCACTCCTCG‑TCGAAAAAACGTa < 2:300288/159‑1 (MQ=255) ttACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCG‑CTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCCGTTTCATCACTCACTCCTCG‑TCGAAAAACCGTAAGCCATGATACAGGATTCTTTCGTTGCTATCCGCCAAATTGATTCGCCGAAATGGAAATAAAAAGCTGGAc > 1:371185/1‑228 (MQ=255) tACGCGGGCCGTTATTCAACCCGTCGCCAAGGATCACCAGCCACTGGGCACCG‑CTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGTTTCATCACTCACTCCTCG‑TCGAAAAAACGTAAGCTATGATACt < 2:648443/168‑1 (MQ=255) gccgcgGGCCGTTAATCAACACGGCGCCAAGGAACACCAGCCACTGGGCACCG‑CTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGTTAACGACCCATGAATGTCCGATGCAAACATCAGTTTCATCACTCACTCCTCG‑TCGAAAAAACGTAAGCTATGATACTGGATTCTGTCGTTGCTATCAGCCAGATTGCTTCGCCGAAAGTGCAATAAAACGCTGGACa < 2:524529/226‑1 (MQ=255) cgcgGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCG‑CTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGTTTCATCACTCACTCCTCG‑TCGAAAAAACGTAAGCTATGATACTGGATTCTGTCGTTGCTATCAGCCAGATTGCTTCGCCGAAAGGGAAATAAAACGCTGGACAGa > 2:530301/1‑228 (MQ=255) ccggggCCGTGATTCAAACCGTGCCCAAGGATCCCCAGCCACTGGGCACCG‑CTTTGGGAAAACACCTCCAGAACCCGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGTTTCATCACTCACTCCTCG‑TCGAAAAAACGTAAGCTATGATACTGGATTCTGTCGTTGCTATCAGCCAGATTGCTTCGCCGAAAGTGCAATAAAACGCTGGACa < 1:530301/223‑1 (MQ=255) gCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCG‑CTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCACACATCAGTTTCATCACTCACTCCTCG‑TCGAAAAACCGTAAGCTATGATACTGGATTCTTTCGTTCCTATCAGCCAGATTGCTTCCCAGAAAGGGACATAAAACCCGGGACaaagcccc > 1:167533/1‑221 (MQ=255) gtgtttcaaccccgccacagggATCCCCCGCCACTGGGCACCG‑CTTTGGGCAAACAACACCAGAACACGTTCCCTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGTTTCATCACTCACTCCTCG‑TCGAAAAAACGTAAGCTATGATACTGGATTCTGTCGTTGCTATCAGCCAGATTGCTTCGCCGAAAGTGCAATAAAACGCTGGACAGAcgctcgct < 1:487238/208‑1 (MQ=255) acacGTCGACAAGGATCCCCAGCCCCTGGGCACCG‑CTTTGGGCAAACAACCCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGTTTCATCACTCACTCCTCG‑TCGAAAAAACGTAAGCTATGATACTGGATTCTGTCGTTGCTATCAGCCAGATTGCTTCGCCGAAAGTGCAATAAAACGCTGGACAGACGCTCGCTGCCACTg < 2:132717/227‑1 (MQ=255) cacGTCCCCAAGGATCCCCAGCCACTGGGCACCT‑CTTTGGGCAAACAACTCCAGAACCCGTTCCGTCGCCGGTAACGCCCCATGAATGTCCGATGCAAACATCAGTTTCATCACTCACTCCTCG‑TCGAAAAAACGTAAGCTATGATACTGGATTCTGTCGTTGCTATCAGCCAGATTGCTTCGCCGAAAGTGCAATAAAACGCTGGACAGACGCTCGCTGCCACTg < 2:83970/226‑1 (MQ=255) ccccccAAGGACCCCCACCCCCGGGCCACCG‑GTTTGGGCAAACAACTCCAGAACACGTTCCGCCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGTTTCATCACTCACTCCTCG‑TCGAAAAAACGTAAGCTATGATACTGGATTCTGTCGTTGCTATCAGCCAGATTGCTTCGCCGAAAGTGCAATAAAACGCTGGACAGACGCTCGCTGCCACTGGAATg < 1:631808/224‑1 (MQ=255) cGCCAAGGATCACCAGCCACTGGGCACCG‑CTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATCCAAACATCAGTTTCATCACTCACTCCTCG‑TCGAAAAAACGTAAGCTATGATACTGGATTCCGTCGTTCCCATCAGCCAGATTGCTTCCCCGAACATGAAAAAAAACCCGGGACAAACGCCCCCTCCCCCCGGAAtt > 1:83970/1‑225 (MQ=255) cccAAGGACCCCCACCCCCCGGGGCCCC‑GTTTGGGCAAACAACTCCAAGACCCGTTCCGTGCCCGGTAACGACCCAGGAATGTCCGATGCAAACATCAGTTTCATCACTCACTCCTCG‑TCGAAAAAACGTAAGCTATGATACTGGATTCTGGCGTTGCTATCAGCCAGATTGCTTCGCCGAAAGTGCAATAAAACGCTGGACAGACGCTCGCTGCCACTGGAATGTTg < 1:168217/227‑1 (MQ=255) | CGTCAAAAACAGACGTTGTTTTTCCAGTAATACCTGTTGCCACGGCGCGGTTATCGGGAAATGCAGCAGCATTTGATCCACTTCGCTGTCGCAGTTGCCGCGCACAGCGATAACCTTATGTGCCACTTCATTAAGCCGTTCAGCGACTTTGGCTGGCGCGTAACCCTCCGGTAAAGCATTACGCGGGCCGTGATTCAACACGTCGCCAAGGATCACCAGCCACTGGGCACCG‑CTTTGGGCAAACAACTCCAGAACACGTTCCGTCGCCGGTAACGACCCATGAATGTCCGATGCAAACATCAGTTTCATCACTCACTCCTCG‑TCGAAAAAACGTAAGCTATGATACTGGATTCTGTCGTTGCTATCAGCCAGATTGCTTCGCCGAAAGTGCAATAAAACGCTGGACAGACGCTCGCTGCCACTGGAATGTTG > NC_000913/2419475‑2419906 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |