| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 149,436 | A→G | 9.2% | S56P (TCC→CCC) | panB ← | 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 149,436 | 0 | A | G | 9.2% | 199.1 / 2.9 | 81 | S56P (TCC→CCC) | panB | 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (25/43); new base G (7/4); total (34/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTG‑GCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGT‑AAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGG > NC_000913/149212‑149661 | ggCACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTG‑GCATACGCCATAAACGGCAGGTCAGCCAGCAGCCGGCGGTTTGGTGCGCCGCCACGTACGGCGGCAGGCTGGGAGGCGGAATCGGCAACGGTGACTGGGAGGGGGGGGt > 1:185074/1‑227 (MQ=255)ggCACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTG‑GCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCCGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGGAGGCGATAGCGGCAACGGGAACGTGCAGGGGGGAGt > 2:612564/1‑227 (MQ=255) gCACGTTCGGTCAGCATTTGTACGGCTTCTACCAGCCACTCACCGCCTTCCATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTG‑GCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCGGTTTGGTGCGCCGCGACGAACGGCGGCCGCTGGGTACGCGCTACCTGCAACGGCGACCGGCCGGGCGGGGTCg > 1:201679/1‑228 (MQ=255) gCACGTTCGGTAAGCATTTGTACGGTTTCTCCCAGCCACTCACCGCCTTCAATTTTGCCCATGTTAGCACCGGCACGCATAACCGTGGCGGCGTTTTCGAAGGCTTGGTCCGGCGTG‑GCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCg < 2:558221/228‑1 (MQ=255) tcacaaTTTGTACGTGTTCTACCGGCCACCCACCTCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCTTTGCGGCGTTTTCGAAGGCTTGGTCCGGCGTG‑GCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGaa < 2:405064/223‑1 (MQ=255) tCAGCTTTTTTCCGGTTTCGCCCCGCCACTCTCCGCCTTCAATTTTGACCCTGTTAGCCCCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTG‑GCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGaa < 1:177705/228‑1 (MQ=255) ttCTACCAGCCACTCACCGCCTTCAATTGTGACCAGGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTG‑GCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCg < 2:66168/227‑1 (MQ=255) tCTACCAGCCACTCACCGCCTTCAATTTTAACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTG‑GCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCACCGGTAACGGGCAGGGTGGAGTGGTGCCCCGGAACCGTCATGCCCAGCGa > 2:684843/1‑227 (MQ=255) cAGCCACTCCCCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTG‑GCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGcc < 2:43703/228‑1 (MQ=255) ccACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTG‑GCATACGCCATAAACGGCAGGTCAGCCCGCCGCAGGCAGTTTGGTGCGCCGCGACGTCCGGCGGCAGGGTGGTAGGCGAAATCGGCAACGGCAACTGGCAGGGCGGAGTCGTGCCCCTGAACCGCCCGGCCCAGCGAATCGccccc > 1:506397/1‑226 (MQ=255) ccACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTG‑GCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGGTTGGTGCGCCGCGACGTACGGCGGCAGTGGGGTAGGCGATGTTGGCGACGGGAACTGGCGGGGGGGGAGCGGGCCCCTGAACCCTCAGGCCCCGCAAAACccccca > 1:597036/1‑226 (MQ=255) ccACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTCCGGCGTTTTCGAAGGCTTGTTCCGGCGTG‑GCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGGTTGGTGCGCCCCGACGCACGGCGGCAGGGTGGTAGGCGAAATCTGACACGGGAACGGGCAGGGGGGGGGCGGGCCACCGAACCGtgcgccccacgcaatcccacac > 1:224319/1‑207 (MQ=255) ccccGGCTTTAATTTTGACCCTGTTTGCCCCGTGACGCATACCCGTGGCGGCGTTTTCGAAGGCTTGTTCCGGCGTG‑GCATGCGCCATAAACGGCAGGGCAGCCAGCAGCAGGAAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCa < 1:140987/226‑1 (MQ=255) cACCGCCTTCAATTTTGGCCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTGTCGAAGGCTTGTTCCGGCGTG‑GCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCa < 2:113801/228‑1 (MQ=255) ccGCCTTCAATTTTGACCATGTTTGCCCCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGGTCCGGCGTG‑GCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATg < 2:597036/228‑1 (MQ=255) ccGCCTTCAATGTTGACCATGTTGGCACCGGCACGCATAACCGTTGCGGCCTTTTCGAAGGCTTGTTCCGGCGTG‑GCATACGCCGTAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATg < 2:289445/228‑1 (MQ=255) ccTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTG‑GCATACGCCATAAACGGCAGGTCAGCCAGCAGCCGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGGACCTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGTCCAGCGAACCGCCCACCAGCATGACg > 2:641715/1‑228 (MQ=255) tCAATTTTGACCATGTTAGCCCCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGGTCCGGCGTG‑GCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTa < 2:54154/228‑1 (MQ=255) ttAGCCCCGGCTCGCATAACCGGTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTG‑GCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCa < 2:229456/228‑1 (MQ=255) gCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTG‑GCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGGGGAGGCGTGCCCCTGGAACGTCCTGCCCCGGCAATCGCCCACCAGCATGGCGTTAAGCCCCTCCTCAACAAAAAGGTTg > 1:482885/1‑228 (MQ=255) gCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTG‑GCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGCGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAAAGCTTg > 2:278855/1‑228 (MQ=255) gCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTG‑GCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGAACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGGGGAGTCGTGCCCCTGAACCGCCATGCCCAGCGAATCGCCCACCAGCATGACGGTAAGCCCTTCATCAGCAAAACGTTTg > 2:595109/1‑228 (MQ=255) gCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTG‑GACTCCGCCATAAACGGCAGGTCAGCCACCAGCAGCGCGTTTGGGGCGCCGCGACGTACGGCGGCAGTGTGGTGGGCGATATCGGCAACGGTGCCTGGACGGGGGGGGCCGTGCCCCTGGACCGTCGTGCCCCGCGACGCGCCCCCCCGCATGACCGTAAGCCCCtcatcatccaacgacctt > 1:576780/1‑216 (MQ=255) gCACGCATAACCGCTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTG‑GCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGCGGTAGGCGATATCGGCAACGGAAACTGGCAGGGTGGAGTCGGGCCCCGGAACCGGCATGCCCCGGGGAACGCACCCCAGCAGGCCGTTAAGCCCTCTATAAGCCAAGAGTTTg > 1:105364/1‑228 (MQ=255) cACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTG‑GCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTgg < 2:651875/228‑1 (MQ=255) cACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTG‑GCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGGAGGCGATATCGGCAACGGTAACTGGCAGGGGGGCGTCGTGCCCCTGAACCGTCATGCCCCGCGAATCGGCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTg > 2:238707/1‑227 (MQ=255) ccGCATAACCGTTGGGGCGTTTTCCGAGGCTTGTTCCGGCGTG‑GCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGTAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGc < 2:185074/227‑1 (MQ=255) acgcagacgctgggcggcgTTTGCGAGGGTGTGTCCCGGCGGG‑GCATACGCGATAAACGGCAGGGCAGGCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGGGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGGCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGc < 1:612564/215‑1 (MQ=255) gCATAACCGTTGCGGCGTGTGCGAGGGCTTGGTCCGGCGTG‑GCAGACGCCATAAGCGGGAGGTCAGCCAGAAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGa < 2:329267/228‑1 (MQ=255) gacccccGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGGG‑GCATACGCCAGAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGa < 2:1712/222‑1 (MQ=255) cttggcggggTTTTCGAAGGGTTGTTCCGGGGTG‑GCATACGGCCTAAGCGGCAGGTCAGCCGGCAGCAGGAAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCAAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCtata < 1:581014/224‑1 (MQ=255) gTTGCCGCGTTTTCGAAGGCTTGTTCCGGCGTG‑GCCTGCGCCATAAACGGCAGGTCAGGCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCtata < 2:589278/227‑1 (MQ=255) cggcgTTTTAGAAGTGTAGTTGCGG‑GTGTGGATCCCCCAGAAACGGCAGGTCAGCCGGCAGCCGGCAGTTGGGGTCGCCGCGACGTACGGGGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCTCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCAt < 1:165121/228‑1 (MQ=255) gcgTTTTCGAAGGCTTGTTCCGGCGTG‑GCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACAAGCATGACGGTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCCATAGTCAGaa > 2:77611/1‑228 (MQ=255) ttCGAAGGCTTGTTCCGGCGTG‑GCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGGGGGGTAGGCGATATCGGCAACGGAAACTGGCCGGGCGGCGGCGTGCCCCTGAACCTTCATGCCCAGCGCAACGCCCACCAGCCTGACGTTAAGCCCTTCATCCGCAAACAGATTTGCGCAGCTATCGTCATAAACggg > 1:506665/1‑227 (MQ=255) cGAAGGCTTGTTCCGGCGTG‑GCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGa < 2:542885/228‑1 (MQ=255) gTTCCGGCGTG‑GCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGTGGAAGCTATAGTCATAACCGGGGATGGTcccgt > 2:671688/1‑227 (MQ=255) gTTCCGGCGTG‑GCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATCGTCATAAGCGGGGATGGTCCCGa > 2:376127/1‑228 (MQ=255) gTTCCGGCGTG‑GCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGGGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGGGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGGCGTTAGACCCTTCCTCAGCAAAGAGTTTGGCGAAACCATAATCaaaacggggtaaggaggcga > 1:112694/1‑212 (MQ=255) gCGTG‑GCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGtt > 2:448172/1‑228 (MQ=255) aaaCGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGGGGAGTCGGGCCCCTGAACCGCCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCCTACGCAAAGAGTTGGGCGAAGCTATAGTCATAAGGGGTGATGGGCGCGAAACGGTTTTTTTCCTGGTTGTa > 1:654711/1‑228 (MQ=255) aaCGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTa < 2:224319/227‑1 (MQ=255) cAGGTCAGGCAGGATGAAGCAGGTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTg < 2:139717/228‑1 (MQ=255) cAGGTCAGCCCGCAGCAGGCAGTTTGGGTCGCCGCGACGTACGGCGGCAGTGGGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACTTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTg < 2:691213/228‑1 (MQ=255) ggTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGGCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAACCGTTTTTTTTCCTGTTTGTACTTCTGcg > 2:592260/1‑227 (MQ=255) gtgagccagcAGCAGGGAAGTGGGTGCGCCGCGACGTACGGCGGGAGTTTGGTAGGCGATATCGGCCACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGACCCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAg < 2:676851/225‑1 (MQ=255) ggtagccgggAGCGGGCAGTTGGGGCCGCCGCGACGTACGGCGGCATTGCGGTAGGCGACATCGGCAACGGTAACTGGCAGGGTTGAGGCGTGCCCCTGAACCGTCATGCCCTGCGAATCGCCCACCAGCATGACGTTAAGTCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAg < 1:609448/225‑1 (MQ=255) gccggccaggAGCCGGCAGGTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAg < 2:654711/224‑1 (MQ=255) tcaggcgggggcgggaggTTTGGTGGGCCGGGGCGGAGGGGGGCCGGTGGGGAGGCAAGATCGGCAACGGTACCTGGCAGGGTGGGTTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGt < 1:684843/211‑1 (MQ=255) ccagcAGCAGGCAGTTTGGTGCCCCGCGACGTGCGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGT‑AAgg < 2:258151/228‑1 (MQ=255) gcagTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATAACGGCAACCGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCTAATCGCACACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGT‑AAGGAGATggtggt < 2:576780/228‑1 (MQ=255) aggTTGGGTGCG‑CGGGAGGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGT‑AAGGAGATggtggt < 2:201679/224‑1 (MQ=255) agTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGT‑AAGGAGATggtggt < 2:112694/226‑1 (MQ=255) agTTTGGGGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGT‑AAGGAGATggtggt < 2:386589/226‑1 (MQ=255) agTTGGGTGCGCCGGCACGTACGGCGGCAGTTGGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGT‑AAGGAGATggtggt < 2:482885/226‑1 (MQ=255) tttGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTtc > 1:20605/1‑128 (MQ=255) ttGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTtca < 2:20605/128‑1 (MQ=255) ggTGGGGCGCGAGGTTGGGGGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGT‑AAGGAGATGGTGGTCGGTTTc < 1:361383/228‑1 (MQ=255) gTGCGCCGCGACGGACGGCGGCAGTGTGGGAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGT‑AAGGAGATGGTGGTCGGTTTc < 2:690298/227‑1 (MQ=255) gcgcCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTACCTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCCTCATCAGCCAACGGTTTGGACAAGCAATAGTCATACGCGGGGGTGGTCGCCAAACGTTTTTTTTCCTGATTTTACTTCTGCAGT‑AAGGAGACGGTGGCAGGTTTCTTa > 1:285278/1‑228 (MQ=255) gcgcCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCGGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGT‑AAGGAGATGGTGGTCGGTTTCATa < 1:448172/228‑1 (MQ=255) cccggGACGTCAGGCGGCAGTGGGGTAGGCGATAGCGGAAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGT‑AAGGAGATGGTGGTCGGTTTCATaa < 2:674804/226‑1 (MQ=255) cgcgACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGGGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCt > 2:466266/1‑142 (MQ=255) cgcgACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTACTGTTTGTAATTCTGCAGT‑AAGGGGATGGTGGTAGGTTTCATAACGt > 2:222922/1‑228 (MQ=255) gcgACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGGGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCta < 1:466266/142‑1 (MQ=255) gacggtaggcggcAGTGTGGTAGGCGATACTGGAAACGGTAACTGGCAGGGTGGAGTAGTTCCCCTGAACCGTCATGCCCAGAGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGT‑AAGGAGATGGTGGTCGGTTTCATAACGTATc < 2:683381/221‑1 (MQ=255) gggcAGTGGGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGT‑AAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAAt < 2:85231/227‑1 (MQ=255) gtgtGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTCTAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGT‑AAGGAGATGGTGGTCGGTTTCATAACGTATCCTGTTAAATTg > 2:695802/1‑225 (MQ=255) tAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGT‑AAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTtgtg < 2:506397/227‑1 (MQ=255) tAGGCGAGATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCTCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATATTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGT‑AAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTtgtg < 2:178565/227‑1 (MQ=255) ggCGATATCGGCAACGGTAACTGGCAGGGTGGAGTGGTGCCCCTGAACCGTCATGCCCAGAGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCCGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGTTCGCGCAACGTTTTTTTTCCGGTTTGTACTTCT‑TAGTCAAGGGAGTGGTGGGCGGTTTCATAACGTATCCGTATaaaattgaatgtgagga > 1:336328/1‑214 (MQ=255) ggCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGT‑AAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGAtgttgt > 2:106410/1‑224 (MQ=255) gCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGT‑AAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTtgtg < 1:106410/224‑1 (MQ=255) tatCGACAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTAATGCCCAGCGAATAGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGCcgc > 2:268082/1‑136 (MQ=255) gagcGGCAACGGTAACTGGCAGGGGGGAGTGGTGCCCATGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCATTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGT‑AAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTc < 1:376127/222‑1 (MQ=255) cGGTTACTGGCAGGGGGGGGTCGTGCCCATGAACCGTCAGGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCAGCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGT‑AAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTAt < 2:105364/227‑1 (MQ=255) ggTAACTGGGCGGGTGGGGTCGGGCCCCTGAACCGTCATGGCCAGCGAAGTCCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGT‑AAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCa < 1:42112/228‑1 (MQ=255) aGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGT‑AAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATc > 2:42112/1‑217 (MQ=255) ggTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAACAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAAAGTTTTTTTTCCTGTTTGTACTTCTGCAGT‑AAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCGGTCTGGCATTTTATCAGTCACATtggtg > 2:255463/1‑228 (MQ=255) aggAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCCTCCGCAAAGAGTTTGGCACACCTATAGTCATAAGCGGTGATGGTCGCGCAACGTTTTTTTTCCTGt > 1:60297/2‑131 (MQ=255) gAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGT‑AAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTgggg > 2:359634/1‑227 (MQ=255) | GGCACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTG‑GCATACGCCATAAACGGCAGGTCAGCCAGCAGCAGGCAGTTTGGTGCGCCGCGACGTACGGCGGCAGTGTGGTAGGCGATATCGGCAACGGTAACTGGCAGGGTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGT‑AAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGG > NC_000913/149212‑149661 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |