Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,206,436 G→A 29.4% intergenic (‑182/‑25) ttdR ← / → ttdA transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4360GA29.4% 39.8 / 4.1 30intergenic (‑182/‑25)ttdR/ttdAtranscriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (3/17);  new base A (4/4);  total (9/21)
Fisher's exact test for biased strand distribution p-value = 1.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTCC‑AGACAAATACAAAAACTGGA‑GTTGCCA‑TG‑ATGAGCGAAA‑GTAATAAGCAACAGGCAGTGAATAAGTTGACAGAG‑ATTGTCGCTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAA  >  NC_000913/3206228‑3206645
                                                                                                                                                                                                                  |                                                                                                                                                                                                                       
tttttataagggaccgtttttCAGCATCTTTAATTATCCGCAGTTTTTATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTACGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTCC‑AGACAAATACAAAAACTGGA‑g                                                                                                                                                                                                     <  1:576063/209‑1 (MQ=255)
tttGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTCC‑AGACAAATACAAAAACTGGA‑g                                                                                                                                                                                                     <  2:177974/228‑1 (MQ=255)
 ttGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGCTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTCC‑AGACAAATACAAAAACTGGA‑g                                                                                                                                                                                                     <  2:217356/227‑1 (MQ=255)
        aaGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTAACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAGATTTTTCA‑AAACAAAAAAAAAAAATGAA‑Gtgataaa                                                                                                                                                                                              >  1:630840/1‑222 (MQ=255)
        aaGGCCAGCTATTCAGCAGCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTTCCATTCCCTGCTCTTGCGCAATATTCATTCTTATTATCGCGTTGATTCACGAACTTTGAATTACATATTCACGGTGGCAAAAAATGTATAACCAAATTTTTAGTAGTAGTTTTGTGCGGGCA‑AATTTTTC‑AAACCAAAATaaaaaaataagtttgggtg                                                                                                                                                                                              >  1:280099/1‑214 (MQ=255)
         aGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTCC‑AGACAAATACAAAAACTGGA‑GTTGcc                                                                                                                                                                                                <  2:630840/224‑1 (MQ=255)
          gcccATCTTTTCAGCAACGTTAATTTTCCGAATTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCGGCTCTGGAGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTCC‑AGACAAATACAAAAACTGGA‑GTTGCCa‑tg                                                                                                                                                                                            <  1:658456/224‑1 (MQ=255)
             ccggtATTCAGCAACGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCTCAAAACATTGCCATTCCCTGCTTTGGCTCAATATTCATTCTTATTATCGCGTTGATTCACGACCTGTTAAGTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTATCC‑AGACAAATACAAAAACTGGA‑GTTGCCA‑TG‑ATGAg                                                                                                                                                                                      <  1:151566/224‑1 (MQ=255)
                cttttCAGCATCGTTAATTTCTCTCAAGTGGGAATAGCGTCGTTTATTTCGGAAATCATTGCCATTCCCTTCTCTGGCGCAAAATTAATTCCTATTATCTCGTTGATTAACGAACTGTTAATTACTTATTCACGGTGGCAAAAAATATAAAACCACATATTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTCC‑AGACAAATACAAAAACTGGA‑GTTGCCA‑TG‑ATGAGCGa                                                                                                                                                                                   <  1:28367/225‑1 (MQ=255)
                 ttttCAGCATTGTTAATTCTCCGCAGTTGTGGAAAGCCTAGTTTTTTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTCC‑AGACAAATACAAAAACTGGA‑GTTGCCA‑TG‑ATGAGCGa                                                                                                                                                                                   <  1:176826/225‑1 (MQ=255)
                 gtttCAGCAACGTTAATTATCCGCAGTTGAGGTAAGCGGAGTGTATTATGTAAAACATTGCCCTGCCCTGCTCTGGCGCAATATTCATTCGTATTTTCGCTTTGATTCACGAACTGTTAATTGCATATTCACGGTGGTAAAAAATATAAAACCACATTCTTAGTGGTAGTTTGTGGCGGTGA‑ATGTTTCA‑AGACAAATACAAAAACTGGA‑GTTGCCA‑TG‑ATGAGCGa                                                                                                                                                                                   <  1:532519/225‑1 (MQ=255)
                         aTCGTTAATTATCCGCAGTTGTGATAAGGGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCAATCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTCC‑AGACAAATACAAAAACTGGA‑GTTGCCA‑TG‑ATGAg                                                                                                                                                                                      <  2:619657/216‑1 (MQ=255)
                             ttAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGA‑ATTTTTTT‑AGTCAAAATAAAAAAAAGGA‑GTTGTCA‑TG‑ATGAggggaacaaaaaaataa                                                                                                                                                                      >  2:372141/1‑212 (MQ=255)
                                        cAGTTGTGATAAGCGCAGTTTATTTCCGAAAACATTGCCATTCCCTGCTTTTGCGGAATATTCATTCTTATTTTCACATTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTCC‑AGACAAATACAAAAACTGGA‑GTTGCCA‑TG‑ATGAGCGAAA‑GTAATAAGCAACAGGCAGTGaa                                                                                                                                                          <  1:35968/228‑1 (MQ=255)
                                              gtaTAAGCGCAGTGTAGTTCGCAAAACATTGACATTCCCTGCTCTGGCGTAATATTCATGCTTATTGTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTGTAGTGGTAGTTTGTGGCGGTGA‑ATTTTACC‑AGACAAATACAAAAACTGGA‑GTTGCCA‑TG‑ATGAGCGAAA‑GTAATAAGCAACAGGCAGTGAATAAGtt                                                                                                                                                    <  2:301718/226‑1 (MQ=255)
                                                   aGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTTT‑ACGAAAATACAAAAACAGGG‑ATTGTCA‑TG‑GTTGGGGAAA‑GGAAAAAGAAACAAACCGTGAaaaacattaaag                                                                                                                                               >  2:365809/1‑220 (MQ=255)
                                                     cgcAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGC‑ATTTTTTC‑AGACAAAAACAAAAAATAGA‑GATTGCA‑CG‑CTTTGTGCAA‑GGAATAAGGAAAAAGCAGTGAAAAAGtatacaaag                                                                                                                                             >  2:351513/1‑220 (MQ=255)
                                                      gcAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTCC‑AGACAAATAc                                                                                                                                                                                                                 <  2:381431/163‑1 (MQ=255)
                                                              tttCGAAAAACTTTGCCATTCCCTGTTTTGGGTCAATATTAATTCTTATTTTCGCGTTAATTCACGAATTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTGAGTGGTAGTTTGTGGCGTTGA‑ATTTTTCC‑AGACAAATACAAAAACTGGA‑GTTGCCA‑TG‑ATGAGCGAAA‑GTAATAAGCAACAGGCAGTGAATAAGTTGACAGAG‑ATTGTCgtt                                                                                                                                   <  1:581423/228‑3 (MQ=255)
                                                                    aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑AATTTTTT‑AAAAAAAAACAAAAAATTGA‑GGTGCCC‑TG‑AGTAGAGGAA‑GTAATAAGAAAAAAACAGAGAATAAAATGACAGAG‑AGTGTCGGTCACttt                                                                                                                             >  2:593041/1‑228 (MQ=255)
                                                                                                  gacaatttcTTATTTTCGCTTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATAAAAAACCACATTTTTAGTGGTATTTTGTGGCGGTGA‑ATTTTTCC‑AAAAAAATAAAAAAACTGGA‑GTTGCCA‑TG‑ATGAGCGAAA‑GTAATAGTCAACAGGCAGTGAATAAGTTGACAGAG‑ATTGTCGCTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCtga                                                                                               <  2:95591/221‑1 (MQ=255)
                                                                                                      cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑AATTTTTC‑ACACAAAAACAAAAAATTGA‑AGTGCCA‑TA‑ATGTGCGGAA‑GGAATAAACAAAAAGGAGTGAAAAAAATGTACGAG‑ATTGTTCCTGAATTT‑TCCGCCCTCAATTTTTCCCGAAAGCCtaagtacc                                                                                          >  2:21262/1‑221 (MQ=255)
                                                                                                            tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTTC‑AATCAAAAACAAAAAATGGA‑GTTGTCC‑TGTATG‑GCGAAA‑GTAAAAAGCAAAAAGCAAGGAATAAGTTGACAGAG‑ACTTTTGGTCAATTT‑ACCGCCCCGAATTCTACCCGCAAGccctaattagggaagg                                                                                    >  2:314439/1‑214 (MQ=255)
                                                                                                                                             atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGA‑ATTTTTTC‑AGAAAAAAACAAAAAATGGA‑GATGCCC‑TG‑ATTGGCGGAA‑AGAATAAACAACAAACAGTGAACAAAATGTCAGAG‑ATTGTCGCTAACTTT‑ACCGCCATGATTTTTACCCGAATACACTCTGACGGGGGGGATCAACTAAACAATATAAACGATTGCGAAAcat                                                   >  2:585501/1‑226 (MQ=255)
                                                                                                                                                    ggggggaaaaaaaTA‑AAAACCACATTTTTAGTGGGTTGTTGGGGGGGGGA‑ATTTTTCCAAGACAAA‑AAAAAAAGTGGG‑AGTGCCAGTG‑TGGGGAGAAA‑TAAAAAATCACGCGGCAGTGAATAATTGGAC‑GAGTATTGGCGTGATTTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAt                                            <  1:365809/221‑1 (MQ=255)
                                                                                                                                                    gggTGGCAAAAAAAAAAAAACCACATTTTAAGTGGTAGTTTGTGGCGGTAA‑ATTTTCCA‑AGAAAAATAAAAAAACTGGA‑GTCGCCA‑TG‑AGGGGCAAAA‑GTAAAAACCAACAGGCAGTAAATAAGTTGAAAGAG‑ATTGTCGCTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGAt                                            <  2:157880/227‑1 (MQ=255)
                                                                                                                                                       tGGAAAAAAATAAAAACCCAGTTTTTTGGTGGTAGTTTGTGGGGGAGA‑ATTTATCA‑AGAAAAATACAAAAACAGGT‑GTGCCAA‑TG‑TGGAGCAAAA‑GTAATAAGCAACAGGAATTAAATAATGTGACAGGT‑ATTGTCCTTAACTTA‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATggg                                         <  2:318877/228‑1 (MQ=255)
                                                                                                                                                         gaaaaaaaaaaaaaACCAGTATTTTGGGGGTAGTTGGT‑GCGGTGA‑AATTTTAC‑AAAAAAAATAAAAAACCGGATGGGGCGA‑GG‑A‑GAGCAAAATGTAATAACACAAGGGCGTTAAATAAGGTAACAGAG‑TTTGCCG‑TGACTTTAACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGTGa                                       <  1:107212/226‑1 (MQ=255)
                                                                                                                                                         aaaaaaaaTATAAAAAACAATTTTTGGGGGTAGTTTGTGGGGGGAA‑ATTTTTCA‑GAAAAAATAAAAAAACTGTG‑GTTGCAA‑TG‑ATGAGTGAAA‑TTAATAAGCAACAGGCAGTTAATAAGTTGAAAGAG‑ATTGTCGTTAATTTT‑ACCGACATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGa                                       <  1:593041/226‑1 (MQ=255)
                                                                                                                                                                                               gggggTAA‑ATTTTCCT‑AAACAAATAAAAAATCTGGA‑GTTGCCA‑TG‑ATGAGAAAAA‑TAAATAAGCAACAGAAAGTGAATAAGTTGACGGAG‑TTTGTCTCTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCaaaa  <  2:243915/224‑1 (MQ=255)
                                                                                                                                                                                                                  |                                                                                                                                                                                                                       
TTTGGCTAAAGGCCAGCTATTCAGCATCGTTAATTATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGA‑ATTTTTCC‑AGACAAATACAAAAACTGGA‑GTTGCCA‑TG‑ATGAGCGAAA‑GTAATAAGCAACAGGCAGTGAATAAGTTGACAGAG‑ATTGTCGCTAACTTT‑ACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAGGATGCCGAAACGTCGTCGATGGGGAAAATTATCTACCATACGATGTTCGACAACATGCAAAA  >  NC_000913/3206228‑3206645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑