Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 3,210,859 | T→G | 19.2% | G27G (GGT→GGG) | rpsU → | 30S ribosomal subunit protein S21 |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,210,859 | 0 | T | G | 19.2% | 128.2 / 2.6 | 74 | G27G (GGT→GGG) | rpsU | 30S ribosomal subunit protein S21 |
Reads supporting (aligned to +/- strand): ref base T (26/26); new base G (16/3); total (45/29) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.33e-02 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGC‑AATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCT‑GGCGGAAGTTCGTCGTCGTGAG‑TTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCG‑CGAAAACGCACGCCGC‑ACTCGTCTGTACTAATTCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTCGTTTATGAA > NC_000913/3210638‑3211085 | ctcACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGC‑AATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGACAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACAATCCGTTAGACGTAACTCTGAGTCGCTTCAAGCGTTCCTGCGAACAAACAGGGGTTCT‑g > 1:61093/1‑228 (MQ=255) tcACTCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGC‑AATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTAAACGGCCCATGCCGGTAATTAAAACACCTGAAACCAAACCGTTCGACCAAGCCCTGCGGTCCTTCAAGCCTGCCCGCGAAAAAACCGGGGtttgg > 1:694766/1‑224 (MQ=255) cACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGC‑AATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTTAGAGTCACATGCCGGTAATTAAAGTACGTGAAAACAAGCCGTTAGACGTAACTCTGCGTAGCTTCAAGCTTTCCGCGGCAAAAGAAGGTGTTTT‑gcg > 2:642754/1‑226 (MQ=255) aCCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGC‑AATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGCTGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCAAATGCCGGGAATTAAGGTACGAGAAACCGAGCCGTTCGACGCAGCTCGCCGGCGCTTCAGACGTTCCTTCGTAAAACCGGGTGTTTT‑GCCg > 2:391539/1‑228 (MQ=255) aCCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGC‑AATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCT‑GGc > 2:422301/1‑227 (MQ=255) aCCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGC‑AATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACAAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGGGTTCT‑gggg > 1:489255/1‑226 (MQ=255) tataAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGC‑AATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAACAGGGGTTAT‑GGCGGCAgg > 1:375476/1‑227 (MQ=255) tataAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGC‑AATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGAAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTGCT‑GGCGGAAGt > 2:427007/1‑228 (MQ=255) tataAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGC‑AATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAACCGTTCGACTTAGCTCTGCGTCCCTTCAACCGTTCCTGCGAAAAACAAGGGGTTCT‑GGCGGAAGt > 1:653326/1‑228 (MQ=255) tataAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGC‑AATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGGGAAAACAAGCCGTTCAACGAAGCCCTGGGGCGCTTCAGGCGTTCCTGCCAAAAAGCAGGGGTTCG‑GGGGGAAGt > 1:343395/1‑228 (MQ=255) ataAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGC‑AATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTAAGAGGCACATGCCGGTAATTAAAGTACGCGAAAACGAGCCGTTCGACGTAGCTCTGCATCGCTTCAAGCGTTTCTGCTGAAAAGAAGGTGTTCT‑GCCGCAAGtt > 2:234339/1‑228 (MQ=255) taAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGC‑AATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCCCATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGCCGTAGCTCTGCGTCGCTTCAAGCGTTCATCCGAAAAAGCAGGGGTTCT‑GCCGGAAGTtc > 2:263820/1‑228 (MQ=255) taAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGC‑AATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGGGTTCT‑GGCGGAAGCtc > 2:385472/1‑228 (MQ=255) aaaaATCCATTTAAAAAAAGGCCGCGGGGTTTTAAAAAGC‑GGAGCAAATGGCAAGAAAAGTCCGGAACAATTGGAAAAAAGCTGGGGTTGATGCAAGCGGAAAACCGAATAAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCT‑GGCGGAAGTtcg < 1:2848/228‑1 (MQ=255) aaGTCCCTTACAAAAACGGCCGCGGTGCTTTACAAAGCAGCAGC‑AATTGACGTAAAATTCCGCCCCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCT‑GGCGGAAGTtcgtc < 2:666349/228‑1 (MQ=255) gTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGC‑AATTGCAGTAAAATTCCGCACCATTTTGAATTAACTTGGCGTTGATACCAGCGGCAAACCGAATTAATCAAAGGCGAGAGGCACATGCTGGTAATTAAAGTAAGTGATAACAAGCGGTTCGAAGTAGCTCTTCTTCGCTTCAAACGTTCCTGCGAACAATCAAGAGATCT‑GAGGGAAGttgccg > 2:99570/1‑222 (MQ=255) gTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGC‑AATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAACCGTTCCTGCGAAAAAGCAGTGGTTCT‑GGCGGAAGTtcctcgt > 2:358697/1‑228 (MQ=255) gTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGC‑AATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGCAATTAAAGAACGTGAAAACGAGCCGTTCAACGTAGCTCTGCGTCCCTTTAAGCGTTACCGCGAAAAAGCAGGGTTTCT‑GGCGGAAATtcgtcgc > 1:662862/1‑227 (MQ=255) gTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGC‑AATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTAAGAGGCACATGCCGGCAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGACGGTGTTCT‑GGCGGAAGGttgtctt > 1:496515/1‑226 (MQ=255) tctgtCAAAAAAGGCCGCGGTGCTTTACAAAGCGGCAGC‑AATGGCAGTAAAATACCGAAACATTTTGAAATAAGCTGGCGTTGATTCCAGCGGCAAACCGAATTAGTCAAGGGTGAGGGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCT‑GGCGGAAGTTcgtcgtcgt < 1:639113/224‑1 (MQ=255) ccTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGC‑AATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTTAGAGGCCCATGCCGGTAATTAAAGTACGGTAAAACGAGCCGGTCAACGTAACTCTACGTCGCTTCAATCGTTCCTCCGCAAAAGCAGGGGTCTA‑GGCGGAAGttcttctttgt > 1:412608/1‑223 (MQ=255) tttCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGC‑AATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGACCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGGTTTCT‑GCCGGAAGTtcgtcgcccggg > 1:147943/1‑222 (MQ=255) ttaaaaaatGGCCCAGGTGCATTACAAGGAAGCAGC‑AAGAGCGGTAAAATCCCACACTTTTTAGAAATAAGCGAGCGTGGATGCGACCGGCAAACCGAATAAGTCAAAGGTGGAAGGGACATGCCGGAAATTAAAGTACGTGAAAACAAGCCGTACGACGTAGCTCTGGGTCGCTTCAATCGTTCCTGCGAAAAAGAAGGTGTTCT‑GGCGGAAGTTCGTCGTCGTGAg < 1:99570/225‑1 (MQ=255) ttCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGC‑AATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGAGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACTAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTACTGCGCAAAAGCAAGGGGTCT‑GGCGGAAGTTCGTCGTCGTggg > 2:672154/1‑226 (MQ=255) ccGGGGTGGTTTACAAAGCAGCAGC‑AAGTGCAGAAAAATTCCGCCCAATTTTGAAATAAGCTGGCGGTTATGCCAGCGGCAAACCAAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAACCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCT‑GGCGGAAGTTCGTCGTCGTGAG‑TTCTATGaaaa < 2:303338/228‑1 (MQ=255) cgGTGCTTTACAAAGCAGCAGC‑AATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGCTCCCAGCGACAAACCGAATTAATCACAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAACCGTTCGCCGGAGCGCTGCGTCGATTCACGAGTTCCTCCGAAAAATCCGGTGTGCT‑GGCGGAGGATCGTGGT‑GGGAGTTTCGATGAAAAAcc > 1:419631/1‑228 (MQ=255) ttttAAACAGCAGCAGC‑AATTGCAGTAAAAACCCGCAACATTTAGAAATAAGCGGGGTTTTATGCGAAGGACAAACGCAATAAATCAAAGGGGGGAGGCTACTGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCT‑GGCGGAAGTTCGTCGTCGTGAG‑TTCTATGAAAAACCGACTa < 1:480795/227‑1 (MQ=255) cTTTACAAAGCAGCAGC‑AATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGGGTTCT‑GGCGGAAGTTCGTCGTCGTGAG‑TTCTATGAAAAAccactta > 2:243325/1‑223 (MQ=255) agcagcagc‑AATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACAAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCT‑GGCGGAAGTTCGTCGTCGTGAG‑TTCTATGAAAAACCGACTACCGCACGt > 2:508117/1‑228 (MQ=255) cagcagc‑aATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCT‑GACGGAAGTTCGTCGCCGTGTG‑TTCTTTGAAAAACGGACCACCGAAccaa > 2:23126/1‑224 (MQ=255) cagcagc‑aATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTACTGCGAAAAAGCAGGAGTTCT‑AGCGGAACTTCGTCGTCGGGAG‑TTCTATGAAAAACCGCCTACCGAACATaa > 2:363051/1‑228 (MQ=255) aaTTGCAGTAAAATTCCGCACCATTTTGAAAAAAGCGGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGTTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCT‑GGCGGAAGTTCGTCGTCGTGAG‑TTCTATGAAAAACCGACTACCGAACGTAAGCGCGCt < 2:372063/228‑1 (MQ=255) atggCAATAAAATTCCGAACAATTTTGAAATAAGCGGGCGTTGACGCCAACGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCT‑GGCGGAAGTTCGTCGTCGTGAG‑TTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTa < 2:652716/225‑1 (MQ=255) ttGCAGTAAAATTCCGCACCATTTTGAAATAAGCGTGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCT‑GGCGGAAGTTCGTCGTCGTGAG‑TTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTaa < 2:148238/228‑1 (MQ=255) aaTTCCGCACCATTTTGAAATAAGCTGCCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTGCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCT‑GGCGGAAGTTCGTCGTCGTGAG‑TTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTg < 2:296387/227‑1 (MQ=255) cACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCT‑GGCGGAAGTCCGTCGTCGTTAG‑TTCTATGAAAAACCGACTACCGAACGAAAGCGCGCTAAAGCTTCTgacgggaac > 2:478544/1‑220 (MQ=255) aaTTTGGAAATAAGGTGGCTGGGAAGCCAGCGGAAAACCGAAATAAACAAGGGGGAGGGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCT‑GGCGGAAGTTCGTCGTCGTGAG‑TTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGt < 1:90696/227‑1 (MQ=255) ttttGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGGACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCT‑GGCGGAAGTTCGTCGTCGTGAG‑TTCTATGAAAAACCGACTACCGAACGTAAGCGCGCGAAAGCTTCCGCAGGCAAACGTCa > 2:379443/1‑228 (MQ=255) aGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCT‑GGCGGAAGTTCGTCGTCGTGAG‑TTCTATGAAAAACCGACTACCGAACGTaa > 2:5268/1‑188 (MQ=255) ggtgagtGCCAGCGGAAAACACGATAAATCAAGGGGGAGGGGCACTGGCCGGTAATTAAAGTAGGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGGGTTCT‑GGCGGAAGTTCGTCGTCGTGAG‑TTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCt < 1:380262/222‑1 (MQ=255) aaTTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTCGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGGGTTCT‑GGCGGACGTTCGTCGTCGTGAG‑TTCTATGAAAAACCGACAACCGAACGAAAGCGCGCTAAAGCTTCTTAAGTGAAACTACACGCGAAGAAACAGGCTCGCCGAAAACACA‑GCCGC‑Accc > 1:618530/1‑226 (MQ=255) aaTTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCT‑GGCGGAAGTTCGTCGTCGTGAG‑TTCTATGAAAAACCGACTACCGAACGTAAg > 2:570531/1‑161 (MQ=255) atgaaTCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCT‑GGCGGAAGTTCGTCGTCGTGAG‑TTCTATGAAAAACCGACTACCGAACGTAAgc < 1:570531/158‑1 (MQ=255) ttaaccaaggggggggcgCACATGCCGGTAATTAAAGTACGTGAAAGCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCG‑GGCGGAAGTTCGTCGTCGTGAG‑TTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCG‑CGAAAACGCACGCCGC‑ACTCGt < 1:263820/211‑1 (MQ=255) ttaacaaaaGGTGGAGGGCACATGCCGGTAATTAAAGTATGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTAGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCT‑GGCGGAAGTTCGTCGTCGTGAG‑TTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCG‑CGAAAACGCACGCCGC‑ACTCGt < 1:385472/222‑1 (MQ=255) aaTCAAGGGGGAGAGCGACATGCGGGTAATTAAAGTAGTTGAAAAGGAGCGTGTGGACGTAGGTCTGGGGCGCTTCAAGGTTTCCTGCGAAAAAGCAGGTGTTCT‑GGCGGAAGTTCGTCGTCGTGAG‑TTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCG‑CGAAAACGCACGCCGC‑ACTCGTc < 1:285829/227‑1 (MQ=255) ggaagagGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCT‑GGCGGAAGTTCGTCGTCGTGAG‑TTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCG‑CGAAAACGCACGCCGC‑ACTCGTCTGTACTaa < 2:105912/224‑1 (MQ=255) aaTTAAAGGTCGGTAAAACGGGCCGTTCGGCGTAGCCCTGCGTTCCTGCAAGCGTTCCTTCGAAAAAGCAGGGGTTCT‑GGCGGGAGTTCGTCGTCGTGAG‑TTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCG‑CGAAAACGCACGCCGC‑ACTCGTCTGTACTAATTCCCCGAGAGCGTTGctc < 1:430552/227‑1 (MQ=255) ttAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCT‑GGCGGAAGTTCGTCGTCGTGAG‑TTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGTGAATAAACCGGCTCG‑CAGAAACGCACGCCCG‑CCACGACTGTCCTAATCcccccaaggcggtcctaccc > 1:601642/1‑212 (MQ=255) aGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCT‑GGCGGAAGTTCGTCGTCGTGAG‑TTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCG‑CGAAAACGCACGCCGC‑ACTCGTCTGTACTAATTCCCCCAGAGCGTTGCTCCCCgcgc > 2:514340/1‑225 (MQ=255) cgggAAAACGAGCCGTTCGACGTAGCTTTGCGTCCCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCT‑GGCGGAAGTTCGTCGTCGTGAG‑TTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCG‑CGAAAACGCACGCCGC‑ACTCGTCTGTACTAATTCCCCGAGAGCGTTGCTCTCCGATCAGa < 2:375476/224‑1 (MQ=255) cGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCT‑GGCGGAAGTTCGTCGTCGTGAG‑TTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGACGAAACTGGCTCG‑CGAAAACGCACGCCCC‑CCTCGCCTGCCGAAATTACCCCAGAACGTTGCCCTCCGATCaacc > 1:514247/1‑225 (MQ=255) cGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCT‑GGCGGAAGTTCGTCGTCGTGAG‑TTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAACCTGGCTCG‑CAAAAACCCACGCCGC‑ACCCGTCCGTCCTAATTCCCCCACAGCGGTACCCTCCgcacacac > 1:255865/1‑221 (MQ=255) cGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCT‑GGCGGAAGTTCGTCGTCGTGAG‑TTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCG‑CGAAAACGCACGCCGC‑ACTCGTCTGTACTAATTCCCCGAGAGCGTTGCTCTCCGATCAGAc > 2:234487/1‑228 (MQ=255) gAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCT‑GGCGGAAGTTCGTCGTCGTGAG‑TTCTATGAAAAACCGACTGCCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCG‑CGAAAACGCAAGCCGC‑ACTCGTCTGCAATAATTCCCCGAGAGGGTTGCTCTCCGATCAGACCgg > 2:37881/1‑227 (MQ=255) gAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCT‑GGCGGAAGTTCGTCGTCGTGAG‑TTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCt > 1:135462/1‑129 (MQ=255) aaaaCGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCT‑GGCGGAAGTTCGTCGTCGTGAG‑TTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCtt < 2:135462/129‑1 (MQ=255) gagctgtgtatgttgcttttggTCGCTTCAAGGGTTCCTGCGAAAAAGCAGGTGTTCT‑GGCGGAAGTTCGTCGTCGTGAG‑TTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCG‑CGAAAACGCTCGCCGC‑ACTCGTCTGTACTAATTCCCCGAGAGCGTTGCTCTCCGATCAGACCGagttgt < 2:290737/205‑1 (MQ=255) atccGTTCGACGTAGCGCTGCTTCGCTGCAAGCGTTCCTGCGAAAAAGCAGGTGTTAT‑GGCGGAAGTTCGTCGTCGTGAT‑TTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCG‑CGAAAACGCACGCCGC‑ACTCGTCTGTACTAATTCCCCGAGAGCGTTGCTCTCCGATCAGACCGagttgt < 2:412608/224‑1 (MQ=255) gTTTGACTTTGCTCTGCGCTGCTTCAAGCGTTCCTGCAAAAAAGCAGGTGTTCT‑GGCGTAAGTTCGTCGTCGTGAG‑TTCTATGAAAACCCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCG‑CGAAAACGCACGCCGC‑ACTCGTCTGTACTAATTCCCCGAGAGCGTTGCTCTCCGATCAGACCGagttgtagttg < 1:150760/227‑1 (MQ=255) gTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGCGTTCT‑GGCGGAAGGTCGCCGTCGTGAG‑TTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTAAAACGTCACCCGCAGAAACAGGCCCG‑CGAAAACGCAC‑CCGCAACCCGTCTGGCCCAATTCCCCGGAAGCGTGCCCCTCCGACCAAAcccggtgtgcgtgta > 1:248100/1‑215 (MQ=255) tAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCT‑GGCGGAAGTTCGTCGTCGTGAG‑TTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCG‑CGAAAACGCACGCCCC‑ACCCGTCGCGACTAATTCCCCGACGGCGTTGGTCTCCCATACGACCggctgtgctgtgtaaggccgg > 1:146289/1‑208 (MQ=255) ggttggCGTCCCTTCAAGCGTTCCTGCGAAACAGCAGGTGTTCT‑GGCGGAAGTCCGTCGTCTTGAG‑TTCGAAGAAAACCCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCG‑CGAAAACGCACGCCGC‑ACTCGTCTGTACTAATTCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTg < 1:598827/222‑1 (MQ=255) ctctGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCT‑GGCGGAAGTTCGTCGTCGTGAG‑TTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCG‑CGAAAACGCACGCCGC‑ACTCGTCTGTACTAATTCCCCGAGAGCGTTGCTCTCCGGTCAGACCGAGTTGTAGTTGTAAGGcctgggt > 2:408866/1‑223 (MQ=255) ctctGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCT‑GGCGGAAGTTCGTCGTCGTGAG‑TTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGCCACGCGAAGAAACTGGCTCG‑CGAAAACGCACGCCGC‑AATCGTCTGTACTAATTCCCCGACAGCGTTGCTCTCCGATCAGACCGCGTTGTAGTTGTAAGGcctggct > 2:79138/1‑223 (MQ=255) ctctGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGGGTTCT‑GGCGGAAGTTCGTCGTCGTGAG‑TTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACAGGCTCG‑CGAAAACGCACGCCGC‑CCGCGGCTGTGCTAATTCCCCGAGAGCGGTTCTCCCCGGCCAAACCCAGTTTTAGTTGTAAGGCCGGGcg > 1:419657/1‑227 (MQ=255) gCGTCGCTTGAAGCGTTCGTGAGAAAAAGCAGGTGTTCT‑GGCGGAAGTTCGTCGTCGTGAG‑TTCTAAGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCG‑CGAAAACGCACGCCGC‑ACTCGTCTGTACTAATTCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTg < 2:264454/222‑1 (MQ=255) gtcgcgttaaaggggtccttggAAAAAGAGGGTGGTCT‑GGCGAGAGTCCGCGCTGGGGAG‑GTTTATGAAAAACCGACCACCCAAAGTAAGCCCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCG‑CGAAAACGCACGCCGC‑ACTCGTCTGTACTAATTCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGa < 1:642754/207‑1 (MQ=255) ggTCGTTTCAAGCGTTCCTGCAAAAAAGCAGGTTTTCC‑GGCGGAAGGTCGGCGTCGTGAG‑TTCTAGGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCG‑CGAAAACGCACGCCGC‑ACTCGTCTGTACTAATTCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGa < 1:408866/227‑1 (MQ=255) gTCGCTTCAAGCGTTCCTGCGAAAGAGCAGGGATGCT‑GGCGGAAGTTCGTCGTCGTAAG‑TTCTAGGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTAAAACGTCACGCGAAGAAACTGGCTCG‑CGAAAACGCACGCCGC‑ACTCGTCTGTACTAATTCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGa < 2:248100/227‑1 (MQ=255) gTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCT‑GGCGGAAGTTCGTCGTCGTGAG‑TTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCG‑CGAAAACGCACGCCGC‑ACTCGTCTGTACCAATTCCCCCAGAGCGTGGCTCCCCGACCCGCCCCGGTTGTAGTTGTAAGGCCGAGCTTCCGaa > 1:41038/1‑228 (MQ=255) cTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCT‑GGCGGAAGTTCGTCGTCGTGAG‑TTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCG‑CGAAAACGCACGCCGC‑ACTCGTCTGTACTAATTCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTGGTTGTAAGGCCGTGCTTCCGAAAGGa > 2:434442/1‑228 (MQ=255) atggTTCCAGCGAAAAAGC‑GGTGTTTTCGGCGGAAGTTCGGCGGAGTGAG‑TTCTATGAAAAACCGACTACCGAACTTAAGCCCGCTAAAGCTTCTGCAGTGAAACGGCACGCGAAGAAACTGGCTCG‑CGAAAACGCACGCCGC‑ACTCGTCTGTACTAATTCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATgcg < 1:400228/225‑1 (MQ=255) gCGAAAAAGCAGGTGTTCT‑GGCGGAAGTTCGTCGTCGTGAG‑TTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCG‑CGAAAACGCACGCCGC‑ACTCGTCTGTACTCATTCCCCGAGAGCGTTCCTCTCCGATCGGACCGGGTTGTAGTTGTAAGGCCGTGCTCCCGAAAGGAACTCGCGGCTTAtt > 2:558931/1‑228 (MQ=255) gtgtTCT‑GGGGGAAGGTCGTCGTGGTGAG‑TTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGGGAAACGTCACGCGAAGAAACTGGCTCG‑CGAAAACGCACGCCGC‑ACTCGTCTGTACTAATTCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTCGTTTATGaa < 1:422301/228‑1 (MQ=255) | CTCACCCTTATAAAAGTCCCTTTCAAAAAAGGCCGCGGTGCTTTACAAAGCAGCAGC‑AATTGCAGTAAAATTCCGCACCATTTTGAAATAAGCTGGCGTTGATGCCAGCGGCAAACCGAATTAATCAAAGGTGAGAGGCACATGCCGGTAATTAAAGTACGTGAAAACGAGCCGTTCGACGTAGCTCTGCGTCGCTTCAAGCGTTCCTGCGAAAAAGCAGGTGTTCT‑GGCGGAAGTTCGTCGTCGTGAG‑TTCTATGAAAAACCGACTACCGAACGTAAGCGCGCTAAAGCTTCTGCAGTGAAACGTCACGCGAAGAAACTGGCTCG‑CGAAAACGCACGCCGC‑ACTCGTCTGTACTAATTCCCCGAGAGCGTTGCTCTCCGATCAGACCGAGTTGTAGTTGTAAGGCCGTGCTTCCGAAAGGAATGCGCGGCTTATTTTCGTTTATGAA > NC_000913/3210638‑3211085 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |