| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,217,528 | T→C | 23.5% | intergenic (+128/+26) | yqjI → / ← aer | PadR family putative transcriptional regulator/fused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,217,528 | 0 | T | C | 23.5% | 66.0 / 2.7 | 37 | intergenic (+128/+26) | yqjI/aer | PadR family putative transcriptional regulator/fused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component |
| Reads supporting (aligned to +/- strand): ref base T (23/3); new base C (2/7); total (25/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.26e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATATCAGTGATGCACAAATAAAAAAGATCATTGCGGTGATCGACCGCGCCGCTTTTGATATTACGCAACTGGATTAATCGCC‑GCATCCGCCAGTGGCGCGGTGCAATT‑GCCGGATGCGACGCTTGAC‑GCGCCTTATCCGGCCTACACCCG‑CTACACACCCCGC‑AGGCCTGATAAGATGCG‑CCAGCATCGCATCAGGCATTGTG‑CTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAGCTCATCCACTGCACGGGTCAGGCTGGAAAGCCCATCGGCCTGTTCCAGCGTTGAATGGCTAATCTGGGCGATCAACTGGGTGACGT > NC_000913/3217324‑3217753 | atatCAGTGATGCACAAATAAAAAAGATCATTGCGGTGATCGACCGCGCCGCTTTTGATATTACGCAACTGGATTAATCGCC‑GCATCCGCCAGTGGCGCGGTGCAATT‑GCCGGATGCGACGCTTGAC‑GCGCCTTATCCGGCCTACACCCG‑CTACACACCCCGC‑AGGCCTGATAAGATGCG‑CCAGCATCGCATCAGGCATTGTG‑CTCCACCCCCCGGATCCGGCATAcc > 2:187792/1‑228 (MQ=255) ttgcaaacaaaaaaaaGATCAGTGGGCACAGCGCCCGGCCCGATTTTGATACTCCGCAAGGAAAGTAGTCGCC‑GCACCCGCCAGTGGCGGGGTGCAATG‑CCCGGATCGGCCGCTGTAC‑CACCCTTACCCGGCCTACCCCCG‑CTACACCCCCCGC‑AGGCCTGATAGTACGGG‑CCAGCATCGAACCGGGAATTGTG‑CTCCAACCGCCGGATCCGGCATACCGATTAATGc < 1:469846/218‑1 (MQ=255) gCACAAATAAAAAAGATCATTGCGGTGATCGACCGCGCCGCTTTTGATATTACGCAACTGGATTAATCGCC‑GCATCCGCCAGTGGCGCGGTGCAATT‑GCCGGATGCGACGCTTGAC‑GCGCCTTATCCGGCCTACACCCG‑CTACACACCCCGC‑AGGCCTGCTAAGATGCG‑CCAGCATCGCATCAGGCATTGTG‑CTCCAACCGCCGGATCCAGCATACCGATTAATGCa > 2:426778/1‑227 (MQ=255) aTCGACCGCGCCGCTTTTGATATTACGCAACTGGATTAATCGCC‑GCATCCGCCAGTGGCGCGGTGCAATT‑GCCGGATGCGACGCTTGAC‑GCGCCTTATCCGGCCTACACCCG‑CTACACACCCCGC‑AGGCCTGATAAGATGCG‑CCAGCATCGCATCAGGCATTGTG‑CTCCAACCGCCGGATCCGGCATACCGATTAATGCAGGACCGTCACCGCGGCTTCCCGTCGCCt > 2:513965/1‑228 (MQ=255) cgccgcTTTTGATATTACGCAACTGGATTAATCGCC‑GCATCCGCCAGTGGCGCGGTGCAATT‑GCCGGATGCGACGCTTGCC‑GCGCCTTATCCGGCCTCCACCCG‑CTCCACACCCCGC‑CGGCCCGATAAGTTGCG‑CCACCCTCTCACCAGGCCATGTC‑CCACTACCGCCGGACTCGGCATCCCGATTGATGCGGTCCCGCCCCCCCGCCCTCCACCCGGCCTGcgcgc > 1:195675/1‑226 (MQ=19) aCGCAACTGGATTAATCGCC‑GCATCCGCCAGTGGCGCGGTGCAATT‑GCCGGATGCGACGCTTGAC‑GCGCCTTATCCGGCCTACACCCG‑CTACACACCCCGC‑AGGCCTGATAAGATGCG‑CCAGCATCGCATCAGGCATTGTG‑CTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCCCGGTGTTTCACCATCGCCg > 2:28875/1‑227 (MQ=255) aCGCAACTGGATTAATCGCC‑GCATCCGCCAGTGGCGCGGTGCAATT‑GCCGGATGCGACGCTTGAC‑GCGCCTTATCCGGCCTACACCCG‑CTACACACCCCGC‑AGGCCTGATAAGATGCG‑CCAGCATCGCATCAGGCATTGGG‑CTCCAACCGCCGGATCCGGCAACCCGATTAATGCAGTACCTTCACCGCGCCTTCCGGTCGCCTGCCCCGGGGTTTCACCACTGCCg > 2:473530/1‑227 (MQ=38) cAAGTGGATTAACCCCCAGCCCCCGCAGGTGGC‑CGGTGCAATTGGCCGGAT‑CGACCTGTGGCGGCGCCTTGACCGG‑CTCCACCCGATTACCCCACCCGC‑GGGCCTTATAAGATGCG‑ACAGCAGCTAAGCAGGCGTTGGG‑CACCAACCTCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGaca < 1:168687/228‑1 (MQ=255) gATTAATCGCC‑GCATCCGCCAGTGGCGCGGTGCAATT‑GCCGGATGCGACGCTTGAC‑GCGCCTTATCCGGCCTACACCCG‑CTACACACCCCGC‑AGGCCTGATAAGATGCG‑CCAGCATCGCATCAGGCATTGTG‑CTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGGTTCACCATCGCCGACCCCTgcg > 2:14252/1‑227 (MQ=255) cgcc‑gcATCCGCCAGTGGCGCGGTGCAATT‑GCCGGATGCGACGCTTGAC‑GCGCCTTATCCGGCCTACACCCG‑CTACACACCCCGC‑AGGCCTGATAAGATGCG‑CCAGCATCGCATCAGGCATTGTG‑CTCCAACCGCCGGATCCGGCATACCGCTTAATGCAGGCCCGTCCCCCCGTCTTCCCGTCGGCTGCCGCGGGGTTTCACCACACCCGACCCCCgcgccccccct > 1:340499/1‑221 (MQ=38) c‑gcATCCGCCAGTGGCGCGGTGCAATT‑GCCGGATGCGACGCTTGAC‑GCGCCTTATCCGGCCTACACCCG‑CTACACACCCCGC‑AGGCCTGATAAGATGCG‑CCAGCATCGCATCAGGCATTGTG‑CTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCCCGTCTTCCAGTCGCCTGGCGCGGGGTTTCACCATACCCGACACCTTCGCACCCCCTTc > 2:280533/1‑227 (MQ=39) tGGCGCGGTGCAATT‑CCCGGATGCGCCGCTTGAC‑GCGCCTTATCCGGCCTACACCCG‑CTACACACCCCGC‑AGGCCTGATAAGATGCG‑CCAGCATCGCATCAGGCATTGTC‑CTCCAACCGCCGGCTCCGGCATCCCGATTAATGCAGTACCGTCACCGCGTCGTCCAGTCGGCTGCCCCGCTGTTTCACCATCGCCCACACCTGCGCCCTCCCCTCCCCCACCCCGGCa > 1:164486/1‑228 (MQ=255) ccGGATGCGACGCTTGAC‑GCGCCTTATCCGGCCTACACCCG‑CTACACACCCCGC‑AGGCCTGATAAGATGCG‑CCAGCATCGCATCAGGCATTGTG‑CCCCAACCGCCGGATCCGGCATACCGa > 2:205805/1‑121 (MQ=255) cGGATGCGACGCTTGAC‑GCGCCTTATCCGGCCTACACCCG‑CTACACACCCCGC‑AGGCCTGATAAGATGCG‑CCAGCATCGCATCAGGCATTGTG‑CCCCAACCGCCGGATCCGGCATACCGAt < 1:205805/121‑1 (MQ=255) ggATGCGACGCTTGAC‑GCGCCTTATCCGGCCTACACCCG‑CTACACACCCCGC‑AGGCCTGATAAGATGCG‑CCAGCATCGCATCAGGCATTCTC‑CTCCAACCGCCGGAACCGGCACACCGCTTAATGCAGCACCGTCACCGCTTCTTCCAGTCGGCTGGCGCGGCGGTTCACCCTCGCCGCCACCTGCGCACACTCTCCCACCAGCCCGGCGTTTTTCTGGGCGACCAg > 1:536138/1‑227 (MQ=255) gcCTTACCCGGCCTACCCCCG‑CTACACACCCCGC‑AGGCCTGATAAGATGCG‑CCAGCCTCGAATGAGGCATTGGG‑CTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAGCTCATCCACTGc < 2:409489/227‑1 (MQ=255) ccTTATCCGGCCTACACCCG‑CTACACACCCCGC‑AGGCCTGATAAGATGCG‑CCAGCATCGCATCAGGCATTGTG‑CTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAGCTCATCCACTGCAc > 2:487449/1‑228 (MQ=255) ccTACACCCG‑CTACACACCCCGC‑AGGCCTGATAAGATGCG‑CCAGCCTCGCATCAGGCATTGTG‑CTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCCCGTCTTCCAGTCGGCTGGGCCGGTGTTTCACCATCTCCGCCACCTGCCCACTCCCTCCCACCCACTCGCCATTGTTCCGGTGGATCAGGTTAAGCTCACCCCCCCGACGGGTccccct > 1:516467/1‑223 (MQ=255) acccccG‑CTACACTCCCCGC‑AGGCCTGATAAGAGGCG‑CAAGACTCGCATCAGGCTTTGTG‑CTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAGCTCATCCACTGCACGGGTCAGGCTGGa < 2:232546/225‑1 (MQ=255) cacacccCCCAC‑GTGCCTGAAAAGCTGCC‑CCAGCATCGCATCAGGCATTGTG‑CCCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAGCTCATCCACTGCACGGGTCAGGCTGGAAAGCCCAt < 2:536138/227‑1 (MQ=255) ccgcccccccaaGGCCTG‑TAAGAAGGGCCCAGCATCGCATCAGGAATTGTG‑CCCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCCCCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCCCCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAGCTCATCCACTGCACGGGTCAGGCTGGAAAGCCCAt < 2:555866/216‑1 (MQ=255) ccccGCAAGGCCGTATAAAATGCC‑CCAGAACCGCATAAGGCA‑TGTGCCACCATCCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAGCTCATCCACTGCACGGGTCAGGCTGGAAAGCCCATCGGcc < 2:340499/228‑1 (MQ=255) ccccGC‑AGGCCTGATAAGATGCG‑CCAGCATCGCATCAGGCATTGTG‑CTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGGGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGGGATCAGGTTAAGCTCCTCCACTGCCCGGGTCAGGCCGGAAAGCCCATCGGCCt > 2:573284/1‑228 (MQ=255) gC‑AGGCCTGATAAGATGCG‑CCAGCATCGCATCAGGCATTGTG‑CTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGCAACCTGCGCCACCTCTTCCACCCACTCGGCATTTTTCTGGGGGATCCGGTTAAGCCCATCCACTCCCCGGTTCAGCCTGTAAACCCCAGCGGCCGGTTc > 1:488414/1‑228 (MQ=255) gC‑AGGCCTGATAAGATGCG‑CCAGCATCGCATCAGGCATTGTG‑CTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAGCTCATCCCCTGCACGGGTCAGGCTGGAAAGCCCATCGGCCTTTTc > 2:207271/1‑228 (MQ=255) gC‑AGGCCTGATAAGATGCG‑CCAGCATCGCATCAGGCATTGTG‑CTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAGCTCATCCACTCCACGGGTCAGGCTGGAAAGCCCATCGGCCCGTTc > 2:390427/1‑228 (MQ=255) gC‑AGGCCTGATAAGATGCG‑CCAGCATCGCATCAGGCATTGTG‑CTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTATCTGGGTGATCAGGTTCAGCTCATCCACTGCACGGGTCAGGCTGGAAAGCCCATCGGCCTCTTc > 2:294720/1‑228 (MQ=255) gC‑AGGCCGGATGAGATGCG‑CCAGCCGCGCATCGGGCTTTGGT‑CCCCACCCGCCGGATCCGGCATACCGATTAATGCAGTTCCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAGCTCATCCACTGCACGGGTCAGGCTGGAAAGCCCATCGGCCTGTTc < 2:516467/228‑1 (MQ=255) aGGCCTGATAAGATGCG‑CCAGCATCGCATCAGGCATTGTG‑CTCCAACCGCCGGATCCGGCATACCGCTTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGGTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGGGATCAGGTTAAGCTCATCCACTGCACGGGGCCGGCGGGGAACGCACTCGGCCTGTTccg > 1:373752/1‑227 (MQ=255) tGATAAGATGCG‑CCAGCATCGCATCAGGCATTGTG‑CTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTTGGTGATCAGGTTAAGCTCATCCACTCCACGGGTCAGGCTGGCAAGCCCATCTGCCTGTTCCACCGt > 2:664953/1‑227 (MQ=255) aTGCG‑CCAGCATCGCATCAGGCATTGTG‑CTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGGTAACCTCCTCCACTGCACGGGTACGGATGGGAAGCCCATCGCCCTGTCCCCGCGTTGCATGGc > 1:587988/1‑228 (MQ=255) aTGCG‑CCAGCATCGCATCAGGCATTGTG‑CTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGACTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGCCATTTTTCTGGGTGTTCAGGTTAAGCTCATCCACTGCACGGTCCAGCCGGGCAAGCCCATCGGCCTGTTCCACCGTTGAATgga > 2:91546/1‑227 (MQ=255) cagctcccgctcAGGCTTTGTG‑CCCCAACCGCCGGATCCGGCATTCCGATTAATGCAGTACCGGCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAGCTCATCCACTGCACGGGTCAGGCTGGAAAGCCCATCGGCCTGTTCCAGCGTTGAATGGCTAATCt < 2:373752/218‑1 (MQ=255) tgtg‑CTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCCCCGCCACCTGCGCAACCTCTTCACCCCGCTCGGCATTTTTCTGGGTGCTCAGGTTAAGCCCATCCCCTGACCCGGTCAGGCTGGACAGCCACTCGGCCGTTTCCAACGTTGAATGGCTCATCTGGGCGGTCAACCggggg > 1:438818/1‑225 (MQ=255) tgtg‑CCCCACCCGCCGGATCCGGCGTACGGTTTAATGCCGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAGCTCATCCACTGCACGGGTCAGGCTGGAAAGCCCATCGGCCTGTTCCAGCGTTGAATGGCTAATCTGGGCGATCAACTGGGTGa < 1:22323/228‑1 (MQ=255) gtg‑CCCCACCCGCCGGATCCGGCATGCCGAGTAAGGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCAATCTCTTCCTCCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAGCTCATCCACTGCACGGGTCAGGCTGGAAAGCCCATCGGCCTGTTCCATCGTTGAATGGCTAATCTGGGCGATCAACTGGGTGa < 2:438818/227‑1 (MQ=255) cTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAGCTCATCCACTGCACGGGTCAGCCTGGCAAGCCCATCGGCCTGTTCCAGCGTTGAATGGATAATCTGGGCGACCACCTGGGTGACGt > 2:613835/1‑227 (MQ=255) | ATATCAGTGATGCACAAATAAAAAAGATCATTGCGGTGATCGACCGCGCCGCTTTTGATATTACGCAACTGGATTAATCGCC‑GCATCCGCCAGTGGCGCGGTGCAATT‑GCCGGATGCGACGCTTGAC‑GCGCCTTATCCGGCCTACACCCG‑CTACACACCCCGC‑AGGCCTGATAAGATGCG‑CCAGCATCGCATCAGGCATTGTG‑CTCCAACCGCCGGATCCGGCATACCGATTAATGCAGTACCGTCACCGCGTCTTCCAGTCGGCTGGCGCGGTGTTTCACCATCGCCGACACCTGCGCACTCTCTTCCACCAGCTCGGCATTTTTCTGGGTGATCAGGTTAAGCTCATCCACTGCACGGGTCAGGCTGGAAAGCCCATCGGCCTGTTCCAGCGTTGAATGGCTAATCTGGGCGATCAACTGGGTGACGT > NC_000913/3217324‑3217753 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |