| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,457,073 | T→A | 22.0% | D233E (GAT→GAA) | gspD → | general secretory pathway component, cryptic |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,457,073 | 0 | T | A | 22.0% | 119.2 / 5.3 | 57 | D233E (GAT→GAA) | gspD | general secretory pathway component, cryptic |
| Reads supporting (aligned to +/- strand): ref base T (11/30); new base A (12/2); total (25/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.71e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAATAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAG‑AAGCAGCAAATTATT‑CATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCA‑TCAGTGGA‑CCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATACCCGGGTTTATTACCTGAAATATGCTAAAGCCACGAATCTGGTGGAAGTGCTA‑ACCGGTGTTTCCGAAAAGCTGAAAGATGAAAAAGGGAATGCGCGTAAGCCCTCCTCTTCTGGCGCG > NC_000913/3456854‑3457295 | aCCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAATAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAG‑AAGCAGCAAATTATT‑CATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCGGTGGCCAGCCCTCCTCTCCGCGAAGATTGCGGCGAAAAAGCGaa > 2:237341/1‑227 (MQ=255)aCCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAATAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAG‑AAGCAGCAAATTATT‑CATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAAc < 2:46113/228‑1 (MQ=255)aCCCCCCAACGTTCTTATTCTGACCGGTCGTGCCTCCCCCATTAAAAAAATGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAG‑AAGCAGCAAATTATT‑CATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAAc < 1:110222/228‑1 (MQ=255) cccTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAAAGAACTGATTTAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAG‑AAGCAGCAAATTATT‑CATCTGGAATATGCGGCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAAcc < 1:506018/228‑1 (MQ=255) cccccaagTTCGTATTCTGACCGGTGGTGCCTCCCCCATTAATAAACTGATGGAAGTTATAAGGCGCGTTGATGTCATCGGCACAGGG‑AAGCAGCAAATTATT‑CATCTGGAATATGCGTCAGCGGAAGATCGCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCGCCTCTCCGCGAAGATTGTGGCGGTTAAGCGAACCaa < 1:442902/221‑1 (MQ=255) cTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAATAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAG‑AAGCAGCAAATTATT‑CATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAACGaa > 2:110222/1‑224 (MQ=255) ttcAACGGTTTTATTCTGGCCGGTCGTGCCTCCACCATTAATAAACTTATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAG‑AAGCAGCAAATTATT‑CATCTGGAATATGCGGCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCaa < 2:126971/225‑1 (MQ=255) ccAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAATAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAG‑AAGCAGCAAATTATT‑CATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTTTCCGCGAAGATTGTTGCGGAAAAACTAACCAAca > 2:700789/1‑227 (MQ=255) tCTTATTCTGACCGGTCGTGCCTCCACCATTAATAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAG‑AAGCAGCAAATTATT‑CATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAGACGAACaaaacatctta > 2:383394/1‑219 (MQ=255) aTTCTGACCGGTCGTGCCTCCACCATTAATAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAG‑AAGCAGCAAATTATT‑CATCTGGAATATGCTTCAGCGGAAGATATCGCCGGGATTCTTAATCAATTAATAAGCGAAACCCACGGAAAAAGCCAAATGCCAACCCCCCTATCCGCGAAGATTGTGGAGGAGaacgagcacaaaccccttcccaca > 1:662859/1‑206 (MQ=255) tCTGACCGGTCGTGCCTCCACCATTAATAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAG‑AAGCAGCAAATTATT‑CATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCA‑TCAg < 2:56925/228‑1 (MQ=255) aCCGGTCGTGCCTCCACCATTAATAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAG‑AAGCAGCAAATTATT‑CATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGAAAAAGCCAGATGCCAGACCTCCTCTCCGCGAAGATTGTCGCGGAAAAGCcaaccaacaccatcatcaccacgcgg > 1:35269/1‑210 (MQ=255) aCCGGTCGTGCCTCCACCATTAATAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAG‑AAGCAGCAAATTATT‑CATCGGGAATATGCGTCAGCGGAAGACCTCGCCGAGATTCTTAATCAATTACACGACGCAAGACACGGTACAGCCCAGATGCACGCCCACCTCTCCCCCAAGATGGTAGCGAAAAAGCAAAcaacacccctaccaacccgggga > 1:339794/1‑206 (MQ=255) cctgttgggCCTCCACCCGTAATAAAAGGATTGAAATCAAAAAGCGGGTTCATTGCATCGGAACAGAG‑AAGCAGGAAATTATA‑CAGCTTGAATATGCGTAAGCGCAAGATCTCGCCGAGATTCTTAATCCATTAATCAGCGAAAGCCGCGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTAGCA‑TCAGTGGaa < 1:121443/220‑2 (MQ=255) tGCCTCCACCATTAATAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAG‑AAGCAGCAAATTATT‑CATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCAAGCCCTCATCTCCGCGAGAATTGTGGCGGAAAAGCGAACCAACACCCTTCtca‑tcacgcggccgggaaa > 1:573236/1‑215 (MQ=255) gCCTCCACCATTAAAAAACTGAATGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAG‑AAGCAGCAAATTATT‑CATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCA‑TCAGTGGA‑CCGGa < 2:573236/224‑1 (MQ=255) ccaccaccaTTAATAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAG‑AAGCAGCAAATTATT‑CATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTACGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCA‑TCAGTGGA‑CCGGAAAAAg < 2:300059/225‑1 (MQ=255) accaTTAATAAACTGATTGAAGTCATAAAGCGCGTTGATGTGATCGGCACAGAG‑AAGCACCAAATTATT‑CATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATAAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGCGGCGGCAAACCGAACCCACTCCTTTATCA‑TCCATGGC‑CCGGACAAAGCag > 2:703292/1‑225 (MQ=255) accaTTAATAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAG‑AAGCAGCAAATTATT‑CATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGAAAAGCGAACCAACCCCCTTCTCT‑CCCCTGGA‑CCCGaaaaacacac > 1:225497/1‑222 (MQ=255) accaTTAATAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAG‑AAGCAGCAAATTATT‑CATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCCCGAAGATTGTGGCGGAAAAACGAAACACCACACTTATCA‑CCCCTGGACCCGGaaaaaaaac > 1:389541/1‑223 (MQ=255) ttAATAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAG‑AAGCAGCAAATTATT‑CATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTACAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTATGCCGGAGAAGCGTACACACTCTCTTATCA‑TCATGGGACCCGGAAAAAGCACGcagc > 2:455359/1‑225 (MQ=255) ttAATAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAG‑AAGCAGCAAATTATT‑CATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCA‑TCAGTGGA‑CCGGAAAAAGCACGCCAg < 2:389541/227‑1 (MQ=255) ttAATAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAG‑AAGCAGCAAATTATT‑CATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGAAAAGCGAACCAAATCCCTTTTCACCCAGCGAC‑CCGGaaaaaaaaccccac > 1:82236/1‑219 (MQ=255) taaACTGATTGAAGTCATAAATCGCAGTGATGTCAGCGGCACAGAG‑AAGCAGCAAATTATT‑CATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTTGCGGATAAGCGAACCAACTCTCTTATCA‑TCAGTGGA‑CCGGAAAAAGCACGCCAGCGCAt < 2:339794/228‑1 (MQ=255) taaACTAATTGAAGTCATAAAGCGCGTTGCGTCCATCGGCCCAGAG‑AAGCAACAAATTATT‑CATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCA‑TCAGTGGA‑CCGGAAAAAGCACGCCAGCGCAt < 2:662859/228‑1 (MQ=255) taaAATGATTGAAATTAGAAAGGGCGGTGGTGCTTACGGCACAGAGAAAGCAG‑AAATTATTCCACCGGGAATAGGC‑TCAGCGGAAGAATCCGCCGGAATTTTTAACCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTAGCGGATAAGCGAACCAACTCTCTTATCA‑TCAGTGGA‑CCGGAAAAAGCACGCCAGCGCAt < 1:53979/228‑1 (MQ=255) aCTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAG‑AAGCAGCAAATTATT‑CATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGAGGCGGATAAGCGAAACCACCCTCTTATCA‑TCATGGGC‑CCGGAAAAAGAACGCCAgagactcac > 2:95279/1‑220 (MQ=255) cTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAG‑AAGCAGCAAATTATT‑CATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCAAACCAAAACCACTATCA‑TCAAGGGA‑CCGGGAAAAGCACACCAggcgagtcat > 2:369813/1‑219 (MQ=255) cTGATGGAAGTCAGAAAGCTCGTTGATTTAATCGGCACAGAG‑AAGCAGCAAATTATC‑CATCTGGAATATGCGTCAGCGGAAGATCCCGCCGAGATTCTTAAGCAATTAACCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCGCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCA‑TCAGTGGA‑CCGGAAAAAGCACGCCAGCGCATCa < 2:606868/226‑1 (MQ=255) aTTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAG‑AAGCAGCAAATTATT‑CATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAAACAACCCCCTTATCC‑TCATTGGA‑CCCGAAAAAGCACGCCAGCGCATACCTTc > 2:128881/1‑227 (MQ=255) gtgTTGAGTTCATCGGCCAAGAG‑AAACTAGAAATTATT‑CATCTGGAATATGCGTAAGCGGAAGATCTCGCCAAGATTCTTAATCAGTTAACCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCA‑TCAGTGGA‑CCGGAAAAAGCACGCCAGCGCAGCCCTTCATTACTGAAAAGCCTTg < 1:299640/226‑1 (MQ=255) cgTTGATGTCATCGGCACAGAG‑AAGCAGCAAATTATT‑CATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAAACCTCTTATCA‑TCAGTGGA‑CCGGGAAAAGCACGCCAGCGCATCACTTCATTACTGTAAAACCttaa > 2:553205/1‑226 (MQ=255) tCGGCACAGAG‑AACCAGCAAATTAAT‑CACCCGGAATACGCGCCAGCGGAAGATCGCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCA‑TCAGTGGA‑CCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAagaga < 1:383394/228‑1 (MQ=255) gCACAGAG‑AAGCAGCAAATTATT‑CATCTGGAATAGGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCA‑TCAGTGGA‑CCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCg < 2:82236/228‑1 (MQ=255) acaGAG‑AAGCAGCAAATTATT‑CATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCA‑TCAGTGGa < 2:565002/165‑1 (MQ=255) agag‑AAGCAGCAAATTATT‑CATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACCCTCTTATCA‑TCCGTGGA‑CCGGAAAAAGCACGCCAGCGCATCACTTCATTACCGCAAAACATTGATGTCGAAGAAAACGagga > 2:205563/1‑228 (MQ=255) aaGCAGCAAATTATT‑CAACTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCA‑TCAGTGGA‑CCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGaggaagga < 2:225497/228‑1 (MQ=255) cagcaAATTATT‑CATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGAAAAACGAACCa > 1:539585/1‑136 (MQ=255) agcaAATTATT‑CATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCaa < 2:539585/136‑1 (MQ=255) caacaggAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAAACAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGATCCAACTCTCTTATCA‑TCAGTGGA‑CCGGAAAAAGCACGCCAGCGCATCACTTCATTTCTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATACCCGGGTttat < 2:592733/223‑1 (MQ=255) gcgtaacactctcccgcaaTTTTTAACCACTTAATCAGCAAAAGTCACGGAAAAAGACATCTGCCCGCCCTCCGATCCGCGAAGAGTGGGGCGGATAAGCGAACCAACTCTCTTATCA‑TCAGTGGC‑CCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATACCCGGTTTTATTACCTGAAATATGATaa < 1:703292/209‑1 (MQ=255) cGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCA‑TCAGTGGA‑CCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTg < 2:158607/158‑1 (MQ=255) gAAAGCCACGTTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGTCGGATAAGCGAACCCACTCTCTTATCA‑TCAGTGGA‑CCGGAAAAAGCCCGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATTCCCGGGTTTATTACCTGAAATATGCTAAAGCCACGAATCTGGTGGAAGTGCTA‑ACCGGTGTTTCCGaa < 2:289460/228‑1 (MQ=255) acaGCCACGGTCAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGAAAAACGACCCACCCCCCTTTTCC‑TCCCCGGG‑ACCGACAAAGCACCCCCGCGCCTCCCCTCATTTCTTGAAAACCCTGCTGTCCAAGAAGGCGGGGACGGACATTCCCGGCGTTATTACCTGCAAACTGCTAAAGCCGCGCACTTGGGGGCAGTACTATACCGTGGTTTCCgca > 1:486887/3‑226 (MQ=255) cGGGAAAAGCCAGATGCCATCCCTCCTCACCGTCAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCA‑TCAGGGGA‑CCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATACCCGGGTTTATTACCTGAAATATGCTAAAGCCACGAATCTGGTGGAAGTGCTA‑ACCGGTGTTTCCGAAAAGCTGaa < 1:553205/228‑1 (MQ=255) ccAGATTCCCGCCCTCCTCACCGCGAAGATTGTGGAGGAAAAGCCAACCAACTCTCTTATCA‑CAAGTGGA‑CCGGAAAAAACACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATACCCGGGTTTATTACCTGAAATATGCTAAAGCCACGAATCTGGTGGAAGTGCTA‑ACCGGTGTTTCCGAAAAGCTGAAAGATGaaa < 1:700789/227‑1 (MQ=255) aTGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCA‑TCAGTGGA‑CCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATACCCGGGGTTATTACCTGAAAAATGCTAAAGCCACGAATCTAGGGGAAGGGCTA‑ACCGGGGTTTCCCAAAAGATGAAAAGTGAAAAAggg > 2:418122/1‑228 (MQ=255) aTGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGAAAAGCGAACCAACTCTCTTATCA‑CCCGGGGG‑CCGGAAAAAAGAACCCAGCGCCACACTTCATTTCTTAAAAGACTTGATGGAGCAAGGAACGGGGAAGGAAAAACCCGGGGTTATTACCTCGAATATGTTAAGACCACGAAACAGGTGGGAGGGTCA‑AACGGGGTTTCCCAAAAgactcaaaaaaaaaaagag > 1:572560/1‑210 (MQ=255) ggCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCA‑TCAGTGGA‑CCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATACCCGGGTTTATTACCTGAAATATGCTAAAGCCACGAATCTGGTGGAAGTGCTA‑ACCGGTGTTTCCGAAAAGCTGAAAg < 2:572560/215‑1 (MQ=255) ccaagccatctcaCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCA‑ACAGTGGA‑CCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATACCCGGGTTTATTACCTGAAATATGCTAAAGCCACGAATCTGGTGGAAGTGCTA‑ACCGGTGTTTCCGAAAAGCTGAAAGATGAAAAAGGGAAt < 2:165578/219‑1 (MQ=255) aGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAAATCTCTTATCA‑TCCGTGGA‑CCGGAAAAAGCACGCCAGCGCATCCCTTCATTACTGAAAAACCTTGGTGTCGAAGAGAGAGAGGAAGGGAATACCCGGGTTTATTACCTGAAATATGCTAAAGCCACGGATCTAGGTGAAGTGCTA‑ACCGGGGTTTTCGAAAAGCTGAAAGATGAAAAAGGGAATg > 2:213694/1‑227 (MQ=255) ctctCCGCGAAGAGTGTGGCGGAAAAGCGAAACAACTCTCTTAGCA‑TCAGTGGA‑CCGGAAAAAGCACGCCAGCGCATCACCTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATACCCGGGTTTATTACCTGAAATATGCTAAAGCCACGAATCTGGTGGAAGTGCTA‑GCCGGTGTTTCCGAAAAGCTGAAAGATGAAAAAGGGAATGCGCGTAAg < 2:129231/228‑1 (MQ=255) cgcgAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCA‑TCAGTGGA‑CCGGAAAAAGCACGCCAGCGCATCACCTCATTACTGAAAAGCCCTGATGTCGAAGAGAGCGAGGAAGGAAAAACCCCGGGTTATTTCCTGAAATAAGCTAAAGCCACGAATATGGGGGAAGGGCTA‑AACGGGGTTTCCCAAAAGCTGCAAAAGAAAAAAgagcgggtccttataaccac > 1:247378/1‑209 (MQ=255) gcgAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCA‑TCAGTGGA‑CCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATACCCGGGTTTATTACCTGAAATATGCTAAAGCCACGAATCTGGTGGAAGTGCTA‑ACCGGTGTTTCCGAAAAGCTGAAAg < 2:247378/199‑1 (MQ=255) cgAAGATTGTGGCGGATAAGCGAACCAACTCCCTTATCC‑TCCGTGGA‑CCGGAAAAAGCACCCCCAGGACTCCCTTCTTTACTGCAAAGCCCTGGTGTCGAAAAAAACACGGAAAGAAATACCCCGGTTTATTACCTGAAAAAATGTAAAGACAAGACAATGGGGGAAGTGCTA‑ACCGCGGTTTTCTGAAAGACCAAAAATGAAACAGGGAAAGGGCGTGAGccccccc > 1:405873/1‑227 (MQ=255) tgGCGGATAAGCGACCCAACTCTCTTATCA‑TCAGTGGA‑CCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATACCCGAGTTTATTACCTGAAATATGCTAAAGCCACGAATCTGGTGGAAGTGCTA‑ACCGGTGTTTCCGAAAAGCTGAAAGATGAAAAAGGGAATGCGCGTAAGCCCTCCTCTTCTGgcg < 2:35269/228‑1 (MQ=255) gCGGATAAGCGAACCAACTCTCTTATCA‑TCAGTGTA‑CCGGAAAAAGCACGCCAGAGCATCACTACATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATACCCGGGTTTATTACCTGAAATATGCTAAAGCCACGAATCTGGTGGAAGTGCTA‑ACCGGTGTTTCCGAAAAGCTGAAAGATGAAAAAGGGAATGCGCGTAAGCCCTCCTCTTCTGGcgcg < 2:405873/228‑1 (MQ=255) | ACCCTCCAACGTTCTTATTCTGACCGGTCGTGCCTCCACCATTAATAAACTGATTGAAGTCATAAAGCGCGTTGATGTCATCGGCACAGAG‑AAGCAGCAAATTATT‑CATCTGGAATATGCGTCAGCGGAAGATCTCGCCGAGATTCTTAATCAATTAATCAGCGAAAGCCACGGTAAAAGCCAGATGCCAGCCCTCCTCTCCGCGAAGATTGTGGCGGATAAGCGAACCAACTCTCTTATCA‑TCAGTGGA‑CCGGAAAAAGCACGCCAGCGCATCACTTCATTACTGAAAAGCCTTGATGTCGAAGAGAGCGAGGAAGGAAATACCCGGGTTTATTACCTGAAATATGCTAAAGCCACGAATCTGGTGGAAGTGCTA‑ACCGGTGTTTCCGAAAAGCTGAAAGATGAAAAAGGGAATGCGCGTAAGCCCTCCTCTTCTGGCGCG > NC_000913/3456854‑3457295 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |