Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,514,936	T→G	17.6%	H41P (CAC→CCC)	rpe ←	D‑ribulose‑5‑phosphate 3‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,514,936	0	T	G	17.6%	147.5 / 2.5	76	H41P (CAC→CCC)	rpe	D‑ribulose‑5‑phosphate 3‑epimerase
Reads supporting (aligned to +/- strand):  ref base T (21/37);  new base G (11/4);  total (35/41)
Fisher's exact test for biased strand distribution p-value = 1.77e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAGACCCGCTTTACAGCCATTTTCTTTAATCAGTTGCAGCGTGCGGTCAACATGCTCGGAGGCTTCTGGATGAAAGGTAATGATGCTGGCACCAGCGGCAGCGAAATCAGGCACAATGCGATCGACGGGTTTCACCATCAGGTGTACGTCGATAGGGGCGGTAATG‑CCATAGTTACGCAAGGATTTCAGCACCATT‑GGCCCAATCGTCAG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTGAGGGGGCAAT‑CA‑AATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGG‑‑GTGAAACGACTCCTGGTTTGTACAAAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTAT  >  NC_000913/3514712‑3515161                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                         ccggACCCGCTTTTCGGCCTTTTTCTTTAAACAGGTGGAGGGGGCGGGCAACATGCTCGGAGGCTTCTGGAGGAAAGGGAATGATGCTGGCCCCAGCGGCAGCGGAATCAGGCAAAATGCTATCGACGGGTTTCACCATCAGGTGTACGTCGATAGGGGCGGTAATG‑CCATAGTTACGCAAGGATTTCAGCACCATT‑GGCCCAATCGTCAG‑ATTGGGAAC‑ATAGTGGt                                                                                                                                                                                                                                       <  1:144542/225‑1 (MQ=255)   aGACCCGCTTTACAGCCATTTTCTTTAATCAGTTGCAGCGTGCGGTCAACATGCTCGGAGGCTTCTGGATGAAAGGTAATGATGCTGGCACCAGCGGCAGCGAAATCAGGCACAATGCGATCGACGGGTTTCACCATCAGGGGTACGTCGATAGGGGCGGTAAAG‑CCAGAATTACGCAAGGCTTTTACGACCAAT‑TGCCCCATGTTGAG‑ATTTGGGAC‑AAAGAGGTTa                                                                                                                                                                                                                                     >  1:610626/1‑228 (MQ=255)         gctgtgcAGGCATTTTCGTTAATAAGGTGCAGCGTGCGGTCAACATGCTAGGAGGCTTCGGGAAGAAAGGTAATGATGCTGGCACCAGCGGCAGCGAAATCAGGCACAATGCGAGCGACGGGTTTCACCATCAGGTGTACGTCGATAGGGGCGGTAATG‑CCATAGTTACGCAAGGATTTCAGCACCATT‑GGCCCAATCGTCAG‑ATTGGGAAC‑ATAGTGGTTATCCATg                                                                                                                                                                                                                               <  2:219102/222‑1 (MQ=255)          cTTTACAGCCATTTTCTTTAATCAGTTGCAGCGTGCGGTCAACATGCTCGGAGGCTTCTGGATGAAAGGTAATGATGCTGGCACCAGCGGCAGCGAAATCAGGCACAATGCGATCGACGGGTTTCACCATCAGGGGTACGTCGATAGGGGCGGTAAAG‑CCATAGTTACGCAAGGGTTTCAGCACCCTT‑GGCCCAATCGTCAG‑ATTTGGGAC‑AAAGGGGGTATcccaga                                                                                                                                                                                                                              >  2:252793/1‑224 (MQ=255)                               tCAGTGGCAGCGTGCGGTCAACCTGGTCGGAGGCTTCTGGATGAAAGGTAATGATGCTGGCACCAGCGGCAGCGAAATCAGGCACAATGCGATCGACGGGTTTCACCATCAGGTGTACGTCGATAGGGGCGGTAATG‑CCATAGTTACGCAAGGATTTCAGCACCATT‑GGCCCAATCGTCAG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGTCAAAATGCACGACATCAg                                                                                                                                                                                                         <  2:475451/228‑1 (MQ=255)                                   ttGCAGCGTGCGGTCAACATGCTCGGAGGCTTCTGGATGAAAGGTAATGATGCTGGCACCAGCGGCAGCGAAATCAGGCACAATGCGATCGACGGGTTTCACCATCAGGTGTACGTCGATAGGGGCGGTAATG‑CCCAAGTTACGCAAGGGTTTCAGACCCATT‑GGCCCAATCGTCAC‑GTTGGGGAC‑ATCAGGGGTATACATGACGTCcaaaaacccaaaaacaagccc                                                                                                                                                                                                     >  1:556135/1‑211 (MQ=255)                                         cGTGCGGTCAACATGCTCGGAGGCTTCTGGATGAAAGGTAATGATGCTGGCACCAGCGGCAGCGAAATCAGGCACAATGCGATCGACGGGTTTCACCATCAGGTGTACGTCGATAGGGGCGGTAATG‑CCAAAGTTACGCAAGGATTTCAGCACCATT‑GGGCCAAACG‑CAGAATTTGGGAC‑AAAGTGGGTTACCCTGACGGCCAAATggcacacatacagccccacggc                                                                                                                                                                                               >  2:575052/1‑207 (MQ=255)                                           tGCGGTCAACATGCTCGGAGGCTTCTGGATGAAAGGTAATGATGCTGGCACCAGCGGCAGCGAAATCAGGCACAATGCGATCGACGGGTTTCACCATCAGGTGTACGTCGATAGGGGCGGTAATG‑CCCTAGTTACGCAAGGATTTTCGCACCATT‑GGCCCAATTGGCGA‑ATTGGGAAC‑ATAAGGGTTTTCCATCAACTTAAAAAGCACgcaacccagccccactcccc                                                                                                                                                                                             >  1:485391/1‑209 (MQ=255)                                            gCGGTCAACATGCTCGGAGGCTTCTGGATGAAAGGTAATGATGCTGGCACCAGCGGCAGCGAAATCAGGCACAATGCGATCGACGGGTTTCACCATCAGGGGTACGTCGATAGGGGCGGAAATG‑CCATAGTTAAGCAAGGATTTCTGCACCATT‑GGCCCCATCGCCAG‑ATTTGGAACAAAAGGGGTTATCC‑TGACGTACAAAAGAACCACATCAA‑CCCCacatccacg                                                                                                                                                                                           >  1:460688/1‑220 (MQ=255)                                             cGGTCAACATGCTCGGAGGCTTCTGGATGAAAGGTAATGATGCTGGCACCAGCGGCAGCGAAATCAGGCACAATGCGATCGACGGGTTTCACCATCAGGGGTACGTCGATAGGGGCGGGAATG‑CCATAGTTACGCAAGGAGTTCAGCACCAAT‑TGCCCCAACGTCCG‑ATTGGGAAC‑ATAAGGGTTATCCCTGACACCAAACAGCACGAAATAAG‑CGCCCGATGACAgg                                                                                                                                                                                          >  1:258437/1‑228 (MQ=255)                                                     aTGCTCGGAGGCTTCTGGATGAAAGGTAATGATGCTGGCACCAGCGGCAGCGAAATCAGGCACAATGCGATCGACGGGTTTCACCATCAGGGGTACGTCGATAGGGGCGGGAATG‑CCATAGTTACGCAAGGATTTCAGCACCATT‑GGCCCAAACGTCAG‑ATTGGGGAC‑CTATAGGTTATCCATGCAGCCAAAATGCACCACATCCA‑CGCCAACTCCCAGGG‑GTTTTg                                                                                                                                                                                  >  1:111057/1‑227 (MQ=255)                                                       gCTCGGAGGGTTCTGGCTGAAAGGTAATGGTGCTGGCACCAGCGGCAGCGAAATCAGGCACAATGCGATCGACGGGTTTCACCATCAGGTGTACGTCGATAGGGGCGGTAATG‑CCATAGTTACGCAAGGATTTCAGCACCATT‑GGCCCAATCGTCAG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCg                                                                                                                                                                                <  2:610626/227‑1 (MQ=255)                                                                   cTGGATGAAAGGTAATGATGCTGGCACCAGCGGCAGCGAAATCAGGCACAATGCGATCGACGGGTTTCACCATCAGGTGTACGTCGATAGGGGCGGTAATG‑CCCTAGTTACGCAAGGATTTCAGCACCATT‑GGCCCAATAGTCAG‑ATTGGGGAC‑ATAGTGGGTATCCATGACGGCAAAATGCACGGAATCAG‑CGCCAACTGCCAGGG‑CTTTTGCGGGATCTTCcccc                                                                                                                                                                    >  2:34079/1‑227 (MQ=255)                                                                     ggttGAAGGGTAATGATGCGGGCCCCGGCGGCAACACAATAAGGCACAATGGGAGCGACGGGTTTCACCATTGGGTTTACGTCGATAGGGGCGGTAATG‑CCATAGTTACGCAAGGATTTCAGCACCATT‑GGCCCAATCGTCAG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAgg                                                                                                                                                                 <  1:403616/225‑1 (MQ=255)                                                                          aaaGGTAATGATGCTGGCCCCAGCGGCAGCGAAATCAGGCACAATGCGATCGACGGGTTTCACCATCAGGTGTACGTCGATAGGGGCGGTAATG‑CCATAGTTACGCAAGGATTTCAGCACCATT‑GGCCCAATCGTCAG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCggg                                                                                                                                                             <  2:448836/227‑1 (MQ=255)                                                                           aaGGTAATGATGCTGGCACCAGCGGCAGCGAAATCAGGCACAATGCGATCGACGGGTTTCACCATCAGGTGTACGTCGATAGGGGCGGTAATG‑CCATAGTTACGCAAGGATTTCAGCACCATT‑GGGCCCATCGTCAG‑CTTGGGTAC‑ACAGGGGTTATCCATGACGGCAAAATGCACGGCATCAA‑GGCCCGCCGCCCGGG‑CTTTTGGGGGATATTTAccacagcgggac                                                                                                                                                           >  1:150368/1‑218 (MQ=255)                                                                               tAACGATGCTGGCACCAGCGGCAGCGAAATCAGGCACAATGCGATCGACGGGTTTCACCATCAGGTGTACGTCGATAGGGGCGGTAATG‑CCATAGTTACGCAAGGATTTCAGCACCATT‑GGCCCAATCGTCAG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAAt                                                                                                                                                       <  2:488903/228‑1 (MQ=255)                                                                                  tgctggtGGCTCGAGCGGCAGCGAAATCAGGACCAATGCGATCGACGGGTTTCAACAGAAGGTTGACTGCGATTGGGGCGGTAATG‑CCATAGTTACGCAAGGATTTCAGCAACATT‑GGCCCAATCGTCAG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCa                                                                                                                                                     <  2:692312/221‑1 (MQ=255)                                                                                      tccggCACCAGCGGCAGCGAAATCAGGCACAATGCGATCGACGGGTTTCACCATCAGGTGTACGTCGATAGGGGCGGTAATG‑CCATAGTTACGCAAGGATTTCAGCACCATT‑GGCCCAATCGTCAG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGa                                                                                                                                               <  2:431226/225‑1 (MQ=255)                                                                                      gtgggCCCCAGGGGCAGCGAAATCAGGCACAATGCGATGGACGGGTTTCACCATCAGGTGTACGTCGATAGGGGCGGTAATG‑CCATAGTTACGCAAGGATTTCAGCAACATT‑GGCCCAATCGGCAG‑ATTGGGAAC‑ATAGGGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCg                                                                                                                                                <  1:317016/224‑1 (MQ=255)                                                                                         ggCACCAGCGGCAGCGAAATCAGGCACAATGCGATCGACGGGTTTCACCATCAGGTGTACGTCGGTAGGGGCGGTAAAGCCCAAAGTAAC‑CAAGGGTTTTAGGCCCATTGGGCCCAAT‑GTCAG‑ATTGGGGAC‑ATAGTGGTTATCCAAGACGTCACAATGCCACACATAAG‑CGCCAACAGCCGGGG‑GCTTTGGGGTATTTTCCCCCCGGGAGGGaaaaaaccgcaaaag                                                                                                                                             >  1:356371/1‑217 (MQ=255)                                                                                         ggCACCAGCGGCAGCGAAATCAGGCACAATGCGATCGACGGGTTTCACCATCAGGTGTACGTCGATAGGGGCGGTAATG‑CCATAGTTACGCAACGATTTCAGCACCCTT‑GGGCCCATCGTCAG‑ATTGGGAAC‑AAATGGGGTTTCCATGACTGCACAATTCACGACATCAGCCCCCCGCTCCCGGGG‑CTTTAG‑GGGATCTTTCCCCCGGCGGGCaaaaaccaccccaaa                                                                                                                                             >  1:599762/1‑217 (MQ=255)                                                                                                   ggagggAAATCATGCAAAGAGCGAACGACGGGTTTGCCCGACGGGTGTACGTCGATAGGGGCGGTAATG‑CCAATGTTACGCAAGGATTTCAGCGCCATT‑GGCCCAATCCTCAG‑ATTGGGAAC‑GTAGTGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTGTCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTGAggg                                                                                                                                   <  2:142385/222‑1 (MQ=255)                                                                                                    gggCGAAATCGGGCCAAATGCGATCGTCGGGGTTCCCCATCAGGTGTACGGCGATAGGGGCGGTAATG‑CCATAGTTACGCAAGGATTTCAGCACCATT‑GGCCCAATCGTCAG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTGAggggg                                                                                                                                 <  1:575052/226‑1 (MQ=255)                                                                                                    gaGCGAAATCAGGCACAATGCGATCGGCGGGTTTCCCCATCAGGTGTACGTCGATAGGGGCGGTAATG‑CCATAGTTACGCAAGGATTTCAGCACCATT‑GGCCCAATCGTCAG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTGAggggg                                                                                                                                 <  2:485391/227‑1 (MQ=255)                                                                                                    gaGCGAAAGCAGGCACAATGCGATCGACGGGTTTCGCCATCAGGTGTACGTCGAGAGGGGCGGTAATG‑CCATAGTTACGCAAGGATGTCAGCACCATT‑GGCCCAATCGTCAG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTGAggggg                                                                                                                                 <  2:533824/227‑1 (MQ=255)                                                                                                    cAGGGAAATCAGGCACAATGCGATCGACGGGTTTCACCATCAGGTGTACGTCGATAGGGGCGGTAATG‑CCATAGTTACGCAAGGATTTCAGCACCATT‑GGCCCAATCGTCAG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTGAggggg                                                                                                                                 <  2:114684/228‑1 (MQ=255)                                                                                                    cAGGGAAATAGGGCACACTGGGATCGCCGGGTTTCACCATCAGGTGTACGTCGATAGGGGCGGTAATG‑CCATAGTTACGCAAGGATTTCAGCACCATT‑GGCCCAATCGTCAG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGGCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTGAggggg                                                                                                                                 <  1:281672/228‑1 (MQ=255)                                                                                                    aaGCGAAATCAGGCACAATGCGATCGGCGGGTTTCACCATCAGGTTTACGTCGATAGGGGCGGTAAGG‑CCATAGTTACGCAAGGATTTCAGCACCATT‑GGCCCAATCGTCAG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTGAggggg                                                                                                                                 <  2:150368/227‑1 (MQ=255)                                                                                                     aGCGAAATCAGGCACGAGGCGATCGACGGGTTTCACCAGCAGGTGTACGTCGATAGGGGCGGTAATG‑GCATAGTTACGCAAGGATTGCAGCACCAGT‑GGCCCAATCGTCAG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGTCAAAATGCACGACAGCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTGAggggg                                                                                                                                 <  2:244271/227‑1 (MQ=255)                                                                                                     aGCGAAATCAGGCACAATGCGATCGACGGGTTTCACCATCAGGTGTACGTCGATAGGGGCGGTAATG‑CCATAGTTACGCAAGGATTTCAGCACCATT‑GGCCCAATCGTCAG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTGAggggg                                                                                                                                 <  2:111057/227‑1 (MQ=255)                                                                                                     aGCGAAATCAAGCACAATGCGATCGACGGGTTTCAACATCAGGTGTACGTCGATAGGGGCGGTAATG‑CCATAGTTACGCAAGGATTTCAGCACCATT‑GGCCCAATCGTCAG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGTCAAAATTCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTGAggggg                                                                                                                                 <  2:376522/227‑1 (MQ=255)                                                                                                         aaaTCAGGCAAAATGCAAACGACGGTTTTTACCATTAGGGTTTCGTCGATAGGGGCGGTAATG‑CCATAGTTACGTAATGATTTCAGCACCAAT‑GACCCAATCGACAG‑ATTGGGAAC‑ATAGGGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCATGCGGGCAAAATCAG‑CCGACAGAATTGAGGGGGCAAT‑c                                                                                                                           <  1:549766/228‑1 (MQ=255)                                                                                                         aaaTCAAGCAAAATGCGATCGCAGGGTTTCACCATCAGGTGTCCGGAGATAGGGGCGGTAATG‑CCATAGTTACGCAAGGATTTCAGCACCATT‑GGCCAAAGCGTCAG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTGAGGGGGCAAT‑c                                                                                                                           <  2:635969/228‑1 (MQ=255)                                                                                                                    aaTGCGATCGACGGGTTTAACCATCAGGTGTACGTCGATAGGGGCGGTAATG‑CCATAGTTACGCAAGGATTTCAGCACCATT‑GGCCCAATCGTCAG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTGAGGGGGCAAT‑CA‑AATACTGtt                                                                                                                <  2:500380/227‑1 (MQ=255)                                                                                                                      tGCGATCGACGGGTTTCACCATCAGGTGTACGTCGATAGGGGCGGTAATG‑CCATAGTTACGCAAGGGTTTCAGCACCATT‑GGCCCAATCGGCAG‑ATTGGGAAC‑ATAGTGGTTAGCCATGCAGTCAAAATGCACGACATCAG‑AGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCCAAATCAG‑CCGACAGCATTGAGGGGGCAAT‑CC‑AATACTGttt                                                                                                               >  2:281388/1‑226 (MQ=255)                                                                                                                      tGCGATCGACGGGTTTCACCATCAGGTGTACGTCGATAGGGGCGGTAATG‑CCATAGTTACGCAAGGAATTCAGCACCATT‑GGCCCAATTGGCAG‑ATTGGGAAA‑ATAGTGGGTATCCATGACGGCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTTCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACGGCAGTGAGGGGGCGAT‑CC‑AATACTGttt                                                                                                               >  2:376552/1‑226 (MQ=255)                                                                                                                        gcggaatgggtataagagacagggTGTAGGTCGATAGGGGCGGTAATG‑CCATAGTTACGCAAGGATTTCAGCACCATT‑GGCCCAATCGTCAG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTGAGGGGGCAAT‑CA‑AATACTGTTTCAt                                                                                                            <  2:548696/205‑1 (MQ=255)                                                                                                                            cGACGGGGTTCACCAGCAGGTGTACGTCGGTAGGGGCGGTAATG‑CCATAGTTACGCAAGGATTTCAGCACCATT‑GGCCCAATCGGCAG‑ATGGGGAAC‑AGAGTGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTGAGGGGGCAAT‑CA‑AATACTGTTTCATCCGc                                                                                                        <  1:372627/227‑1 (MQ=255)                                                                                                                              aCGGGTTTCACCATCAGGTGTACGTCGATAGGGGCGGTAATG‑CCATAGTTCCGCAAGGGTTTCAGCACCATT‑GGCCCAATCGTCAG‑ATTGGGAAC‑ATAGGGGTTTTCCATTACGGCCAAAATCCCCAAATCAA‑CGCCCACGGCCAGGGCCTTTTCCGCTA‑CTTTCCCCACGCGGGCAAAACCAC‑CCGCACCAACTAGGGGGGCAAT‑CA‑CACACTGtttacaccgctct                                                                                                     >  1:27453/1‑218 (MQ=255)                                                                                                                                       aCCATCAGGTGTACGTCGATAGGGGCGGTAATG‑CCATAGTTACGCAAGGATTTCAGCACCATT‑GGCCCAATCGGCAG‑ATTGGGAAC‑ATAGGGGTTATCCATGACGTCAAAAAGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGAGAAATCAG‑CCGGAAGCATTGAGGGGGGAAT‑CA‑AAAACCTTTTCAGACCCTTCTCCTTggaa                                                                                            >  1:461032/1‑225 (MQ=255)                                                                                                                                        ccATCAGGTGCACGTCGATAGGGGCGATAATG‑CCATAGTTACGCAAGGATTTCAGCACCATT‑GGCCCAATCGTCAG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTGAGGGGGCAAT‑CA‑AATACTGTTTCATCCGCTTCTCCTTGAGaa                                                                                           <  2:129379/228‑1 (MQ=255)                                                                                                                                                  tACGTCGATAGGGGCTGTAATG‑CCATAGTTACGCAAGGATTTCAGCACCATT‑GGCCCAATCGTCAG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTGAGGGGGCAAT‑CA‑AATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTc                                                                                 <  2:461032/228‑1 (MQ=255)                                                                                                                                                  tACGTCGATAGGGGCGGTAATG‑CCATAGTTACGCAAGGATTTCAGCACCCTT‑GGCCCAATCGTCAG‑ATTGGGAAA‑AAAGTGGTTATCCATGACGTCAAAATGCACGACCTCAG‑CGGCAGCTGCCAGGG‑CCTTTGCGGGATATTACCCCCAGCGGGCAAAATCAG‑CCCCCAGACTTTGGGGGGCAATACC‑AAAAC‑GGTTCATCCCCCTTCCCTTGGAAATAAttttttt                                                                                 >  1:120877/1‑227 (MQ=255)                                                                                                                                                   aCGTCGATAGGGGCGGTAATG‑CCATAGGTACGCAAGGATTTCAGCCCCATT‑GGCCCAATCGTCAG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTGAGGGGGCAAT‑CA‑AATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTcg                                                                                <  2:120877/228‑1 (MQ=255)                                                                                                                                                         aTAGGGGCGGTAATG‑CCATAGTTACGCCAGGATTTCAGCACCATT‑GGCCCACTCGTCAG‑ATTGGGAAC‑CTAGGGGTTATCCCTGACCTCAAAATGCACGACATAAG‑CGCCCGCCTCCCGGG‑CTTTTGCGGACTCTTCACCCAACCCGGCCAAAACAG‑CCCACCACATTTAGGGGGGCAT‑CACAACACT‑TTTCCCACCCCTCTCCCTTAGAATTATTTTTTTCCgg‑‑ggg                                                                        >  1:348665/1‑226 (MQ=255)                                                                                                                                                         aTAGGGGCGGGAATG‑CCATAGTTACGCAAGGATTTCAGCACCATT‑GGCCCAATCGTCAG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGTCCAAATGCACGACATCAG‑CGCCAGCATCCCAGG‑CTTTTGCGGTAACTTTACCCAAGCGGGCAAAATCAG‑CCGGCCAGATTTAGGGGGGAAT‑CA‑CATACTGTTTCATCCGCCTTTCCTCGAGCATTAttttttttcgcggg                                                                          >  1:62158/1‑220 (MQ=255)                                                                                                                                                                 ggTAATG‑CCATAGGTACGCAAGGATTTCAGCACCATT‑GGCCCAATCGTCCG‑ATTGGGAAC‑ATAGGGGTTAACCAAGACGTCAAAATGCCCGAAATTAG‑CGCCACATGCCAGGG‑CTTTTGCGGTAACTTACCCCAAGGCGGCCAAATCAGCCCGAC‑GCATTGGGGGGGCAAT‑CA‑ACTTCTGTTTCATCCGCTTCCCCTTTAGAATTATTTTTTTGCGG‑‑GGTAACCGACt                                                                >  1:538558/1‑228 (MQ=255)                                                                                                                                                                  gTAATG‑GCTTAGTTAAGAAAGGAGTTCAGCCCTATT‑GGCACAATCGTCAG‑ATTGGGAAC‑AGAGGGGTGATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTGAGGGGGCAAT‑CA‑AATCCTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGG‑‑GTGAAACGACTc                                                               <  1:541379/228‑1 (MQ=255)                                                                                                                                                                           atgtttACGCAAGGATTTCAGCCCCATT‑GGCCCAAGCGTCAG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTGAGGGGGCAAT‑CA‑AATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGGGTGTGAAACGACTCCTGGt                                                          <  1:34079/223‑1 (MQ=255)                                                                                                                                                                              gTTACGCAAGGATTTCAGCACCATT‑GGCCCAATCGTCAG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCCGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGCCAGAATTGAGGGGGCAAT‑CA‑AATACTGTTTCATCCGCTTCGCCTTTGGAATTATTTTTTCGCGG‑‑GTGAAACAACTCCTGGTTTGTAc                                                    >  2:560655/1‑228 (MQ=255)                                                                                                                                                                                  cGCAAGGATTTCAGCACCATT‑GGCCCAATCGTCAG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTGCGGGGGCAAT‑CA‑AATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCgg‑‑g                                                                          >  1:117135/1‑202 (MQ=255)                                                                                                                                                                                  cGCAAGGATTTCAGCACCATT‑GGCCCAATCGTCAG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTGAGGGGGCAAT‑CA‑AATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGG‑‑GTGAAACGACTCCTGGTTTGTACaaa                                                 >  2:490454/1‑227 (MQ=255)                                                                                                                                                                                  cGCAAGGATTTCAGCACCATT‑GGCCCAATCGTCAG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTGAGGGGGCAAT‑CA‑AATACTGTTTCATCCGCTTACCCTTGAGAATTATTTTTTCGCGG‑‑GTGAAACGACTCCTGGGTTGTAaaaaa                                                >  1:103121/1‑227 (MQ=255)                                                                                                                                                                                   gCAAGGATTTCAGCACCATT‑GGCCCAATCGTCAG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTGAGGGGGCAAT‑CA‑AATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGG‑‑Gt                                                                         <  2:117135/202‑1 (MQ=255)                                                                                                                                                                                    cAAGGATTTCAGCACCATT‑GGCCCAATCGTCAG‑ATTGGGTAC‑ATAGAGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTCAGGGGGCAAT‑TT‑AATACTGTTTCATCCGCTTCTCCTTTCGAATTATTTTTTCGCAG‑‑CCGAACCGACTCCTCGTATGTACAAAGc                                               >  2:246731/1‑227 (MQ=255)                                                                                                                                                                                       ggATTTCAGCACCATT‑GGCCCAATCGTCAG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGGCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTGAGGGGGCAAT‑CA‑AATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGG‑‑GTGAAACGACTCCTGGTTTGTACAAAGCcagc                                           <  2:599762/228‑1 (MQ=255)                                                                                                                                                                                         aTTTCAGCACCATT‑GGCCCAATCGTCAG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTGAGGGGGCAAT‑CA‑AATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGG‑‑GTGAAACGACCCCTGGTTTGTACAAAGCcaacag                                         >  2:392026/1‑228 (MQ=255)                                                                                                                                                                                                cACCATT‑GGCCCAATCGGCAG‑ATTTGGAAC‑TTAGTGGTTAACCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCTGGCAAAATCAG‑CCGACAGAATTGAGGGGGCAAT‑CA‑AATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGG‑‑GTGAAACGACTCCTGGTTTGTACAAAGCCAGCAGTTCGTcc                                  <  2:667601/228‑1 (MQ=255)                                                                                                                                                                                                 aCCATT‑GGCCCAATCGTCAG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACACAGGCGGGCAAAATCAG‑CCGACAGAATTGAGGGGGCAAT‑CA‑AATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGG‑‑GTGAAACGACTCCTGGTTTGTACAAAGCCAGCAGTTCGTcc                                  <  2:64703/227‑1 (MQ=255)                                                                                                                                                                                                   cATT‑GGCCCAATCGTCAG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTGAGGGGGCAAT‑CA‑AATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGG‑‑GTGAAACGACTCCTGGTTTGTACAAAGCCACCAGTTCGTCCAcc                               >  2:275831/1‑228 (MQ=255)                                                                                                                                                                                                   cATT‑GGCCCAATCGTCAG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTGAGGGGGCAAT‑CA‑AATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGG‑‑GGGAAACGACTCCTGGTTTGTACAAACCCAGCAGttattc                                   >  1:64703/1‑220 (MQ=255)                                                                                                                                                                                                    aTT‑GGACAAATCGGCAG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTGAGGGGGCAAT‑CA‑AATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGG‑‑GTGAAACGACTCCTGGTTTGTACAAAGCCAGCAGTTCGTCCAc                                <  2:27453/226‑1 (MQ=255)                                                                                                                                                                                                     tt‑GGCCCAATCGTCAG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTGAGGGGGCCAT‑CA‑AATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGG‑‑CTGAAACGACTCCTGGTTTGTCCAAAGCCATCAGTTCGTCCACCt                              >  2:372331/1‑227 (MQ=255)                                                                                                                                                                                                              aTCGTCAG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTGAGGGGGCAAT‑CA‑AATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCTCGG‑‑GTGAAACGACTCCTGGTTTTTTCAAAGCCAACAGTTCGTCCACCTTTTTAcat                      >  1:372201/1‑225 (MQ=255)                                                                                                                                                                                                              aTCGTCAG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTGAGGGGGCAAT‑CA‑AATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGG‑‑GTGAAACGACTCCTGGTTTGTACAAAACCAGCAGTTCGTCCACCTTTTTCCgt                      >  2:9815/1‑227 (MQ=255)                                                                                                                                                                                                                 gTCAG‑TTTGGGAAC‑ATAGGTGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTGAGGGGGCAAT‑CA‑AATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGG‑‑GTGAAACGACTCCTGGTTTGTACAAAGCCAGCAGTTCGTCCACCTTTTTACGTGTgc                  <  1:379344/228‑1 (MQ=255)                                                                                                                                                                                                                    aG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTGAGGGGGCAAT‑CC‑AATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTAGCGG‑‑TGGAAACGACTCCTGGTTTGTACAAAGCCAGCAGTTTGTCCACCTTTTTCAGTGGgccgc               >  1:94347/1‑228 (MQ=255)                                                                                                                                                                                                                       aTTGGGAAC‑ATAGTGGTTAACCATGACCTCAAAATGCACGACATCAT‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGTCGGGCAACATGAG‑CCGACAGAATTGATGGGGCAAT‑CA‑AATACTGTTTCCTCCGCTTCTCCTTGAGAATTATTTTTTCCCGG‑‑TTGAAACACCTCCCGGTTTTTACAAACCCAACATTTCGTACCCCTTTTTACgtttcaccccc             >  1:661514/1‑219 (MQ=255)                                                                                                                                                                                                                         ttggAAC‑ATAGTGGTTAACCATGACCTCAAAATGACAGACAGTAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCAAGGCGGGCAAAATCAG‑CCGACAGAATTGAGGGGGCAAT‑CA‑AATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGG‑‑GTGAAACGACTCCTGGTTTGTACAAAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGtt           <  2:702086/226‑1 (MQ=255)                                                                                                                                                                                                                         tGGTAAC‑ATAGTGGTTATCCATGACGTCAAAATGCACGGAATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTGAGGGGGCAAT‑CA‑AATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGG‑‑GTGAAACGACTCCTGGTTTGTACAAAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGtt           <  2:46750/228‑1 (MQ=255)                                                                                                                                                                                                                         tGGGAAC‑ATGGTGGTTACCCATGACGTCAAAAGGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCTGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTGAGGGGGCAAT‑CA‑AATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGG‑‑GTGAAACGACTCCTGGTTTGTACAAAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGtt           <  2:460688/228‑1 (MQ=255)                                                                                                                                                                                                                         tGGAAAC‑ATAGTGGTTACCCATGACGTCAAAATGCCCGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTGAGGGGGCAAT‑CA‑AATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGG‑‑GTGAAACGACTCCTGGTTTGTACAAAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGtt           <  2:103121/228‑1 (MQ=255)                                                                                                                                                                                                                          ttggaaactggTGGTTATCCTTGACGTAAAAATGCCCGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCCCCCAGGCGGGCAAAATCAG‑CCGACAGAATTGAGGGGGCAAT‑CA‑AATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGG‑‑GTGAAACGACTCCTGGTTTGTACAAAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGtt           <  1:49467/218‑1 (MQ=255)                                                                                                                                                                                                                                  tAGTGGGTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTGAGGGGGCAAT‑CA‑AATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCACGG‑‑GTGAAACGCCTCCTGGTTTGTAAAACACCAGGAGTTCGTCCACCTTTTAACGTTAGCCGCCGtttcgggtta   >  1:327746/1‑220 (MQ=255)                                                                                                                                                                                                                                   aGTGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTGAGGGGGCAAT‑CC‑AATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGG‑‑GTGAAACGACTACCGGTTTGTACAAATCCAACAGTTGGTCCACCCTTCTACGTGTGCCGCCGTGCCTGCTtat  >  2:204487/1‑228 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                         CCAGACCCGCTTTACAGCCATTTTCTTTAATCAGTTGCAGCGTGCGGTCAACATGCTCGGAGGCTTCTGGATGAAAGGTAATGATGCTGGCACCAGCGGCAGCGAAATCAGGCACAATGCGATCGACGGGTTTCACCATCAGGTGTACGTCGATAGGGGCGGTAATG‑CCATAGTTACGCAAGGATTTCAGCACCATT‑GGCCCAATCGTCAG‑ATTGGGAAC‑ATAGTGGTTATCCATGACGTCAAAATGCACGACATCAG‑CGCCAGCTGCCAGGG‑CTTTTGCGGTATCTTCACCCAGGCGGGCAAAATCAG‑CCGACAGAATTGAGGGGGCAAT‑CA‑AATACTGTTTCATCCGCTTCTCCTTGAGAATTATTTTTTCGCGG‑‑GTGAAACGACTCCTGGTTTGTACAAAGCCAGCAGTTCGTCCACCTTTTTACGTGTGCCGCCGTTGCTGCTTAT  >  NC_000913/3514712‑3515161

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑