| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 4,163,577 | T→C | 6.3% | intergenic (‑308/‑61) | trmA ← / → btuB | tRNA m(5)U54 methyltransferase, SAM‑dependent, tmRNA m(5)U341 methyltransferase/vitamin B12/cobalamin outer membrane transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,163,577 | 0 | T | C | 6.3% | 191.5 / 2.1 | 71 | intergenic (‑308/‑61) | trmA/btuB | tRNA m(5)U54 methyltransferase, SAM‑dependent, tmRNA m(5)U341 methyltransferase/vitamin B12/cobalamin outer membrane transporter |
| Reads supporting (aligned to +/- strand): ref base T (32/34); new base C (3/2); total (35/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01 | |||||||||||
CTACGTCTGGACAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACTGTGCTTGCACCAACCACCGTTGTGACC > NC_000913/4163355‑4163787 | gttcGTCTGGGCATTAACTTGTGTGACTCGGTAGTATCCAGTTGCCGGCCGGGGGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCGCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCAACTGGTTCtcatca < 1:688340/225‑1 (MQ=255)cgaCGTCTGGCAATTAATTTGGTACACCCTGTAGCTTCCCGGTGCCGGGCCTGTGAGTTAATAGGGAATCCAGTGGGAATCTGGGGCTGACGGGCCGCGGTAAGGAAAGGTGCGATGATTTGGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCtcatca < 1:503396/226‑1 (MQ=255) agcGTTTGGAAAGTAACTTGTTACAACCTGTAGCCTCCACTTGCCGGTCCTGTGAGTTAATGGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCtcatca < 2:416303/225‑1 (MQ=255) cGTCTGGACAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCATGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTACGGATAGGTGCGATGATTGCGTTATGCGGCCACTGCCGTTCGGTGCGAAGTCATCATCTGTTAGTATCTTAGCTACCCCTCCAAGCCCGAAGACCTGCCGGCAAACGTAGCATCTGTTTCTCATCTTcg > 2:238807/1‑228 (MQ=255) cTGGACAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACGGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGCCCAACGTCGCACCAGGTtctctcactcgcgg > 2:681886/1‑218 (MQ=255) gcAGTAACTTGTTACAACCTTGAGCATCCAGTTGCCGGTCGTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAata < 2:329550/227‑1 (MQ=255) cAGTAATGTGTTACAAGCTGTAGCATCCACTTGCCGGTCCTGGGAGTTAAAAGGGAATCCAGTGCGAGTCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGTACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAata < 2:292221/227‑1 (MQ=255) tAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGCCCAAAGTCGCCTCTGGGTAccatcaccccgcaaattcct > 1:416303/1‑214 (MQ=255) tACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTATTAGGGAATCCAGTGCGAATCTGGAGCTGCCGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGTCATTCGTTGGGAAGTCATCATCTCTTAGTATCTGAGATACCCCTCCAAGCCCGAAGACCGGCCGGCCAACGTCGCACCAGGTACCCACCATCCCGCAAAATTGTTGACAccc > 1:329550/1‑227 (MQ=255) tAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCTAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATcc < 2:542891/228‑1 (MQ=255) tAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATc > 2:79315/1‑227 (MQ=255) aGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATcc < 1:79315/227‑1 (MQ=255) aTCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCcttct > 2:40542/1‑228 (MQ=255) cACTTGCCGGTTCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGGAAGGAGCGATTATTGCGTTATGCGGACATGGCCATCCGGTGGGAAGTGTTCATCTCTTAGTATTTTAGATATCACTCCAAGCCCGAAGACCTCCCGGAAAACGACCCATCTGGTCATCACCATCGCGTAATATTGATAAAAACTTCCGCACCCTtcttct > 1:665902/1‑228 (MQ=255) aCTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCACCCTTCTTCTa > 2:556836/1‑228 (MQ=255) ttGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCCCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTAtt > 2:343292/1‑228 (MQ=255) gggccTGGGAGTTAATGGGGAATTCAGTGGGGAGGATGGGCTGACGCGCAGGTGTAAAGGAAGGGTCGATTATTGGGTTAGGCGGACACTGCCATTCGGTGGGAAGTCCTCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAAACCTGCCGGCGAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCAGCATCCTTCTTCTATTGTGGa < 1:678398/225‑1 (MQ=255) ggTCCTTTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTTACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCACCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTgg < 2:465219/227‑1 (MQ=255) ccTGTGAGTTAATAGGGAATCCAGGGCGAATCTGGTGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCTGACCCTGCCCTTCGGTGGGAAGGCATCTTCTCTTAGTATCTTAGATAACCCTCCAAACCCGAAGACCTGCAGGCCAACGTCTCCTCCGGTTCTCAACAACCCGTAACATTGACGAAACCCACGGccccatttcaccatttaggatgc > 1:302261/1‑206 (MQ=255) aGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACa < 2:276935/228‑1 (MQ=255) gTTAATGGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGTAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACa < 2:92353/227‑1 (MQ=255) gTTAATAGGGAATTCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACa < 2:663990/227‑1 (MQ=255) gTTAAAGGGGAATCCAGTGGGAATCTGGAGCTGACGCGCCGCGGGAAGGAAAGGTGTGATGATTGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCCTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACa < 1:681886/227‑1 (MQ=255) aaaggggAATCCATGGTGAATCTGGGGCTGACGCGCAGGGGGAAGGAAAGGTGCGATGGTTGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATg < 1:40542/223‑1 (MQ=255) gggAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGAtt > 2:169564/1‑226 (MQ=255) gggAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGGGGGAAGTCATCATCTCTTAGTATCTTTGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCCTCATCCCGTAACATTGCGGAAACCTGCGGCATCCTTCTTCTATTTTGCTTGCTTTACAATGATaaa > 1:201538/1‑228 (MQ=255) ggAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGAGCGATGATTGCGTGATGCGGACACTGCCATTCGGTGGAAAGTCATCATCTCTCAGTATCTTGGATACCCCTCCAAGCCCGAAGCCCTGCCGCGCAACGTCGCATCTGGTTCTCATCACCCCTGAATATTGATGAAACCTGCGGCAACCGTCTTCTATTGGCGATGCTTTACAGTGAttt > 2:306291/1‑225 (MQ=255) gAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGCTGAAACCTGCGCCCTCCTTCTTCTTTTGTGGCTGCTTTCAAATGATTaaa > 1:499035/1‑227 (MQ=255) aTCCAGTGCGAGTCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGCTGATTGCGTTATGCGGACACTGCCATTCGGTGGGAAGGCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCAGGCCCACGTCGCATCTGGTTCTCCTCATCGCCGAATATCGATGAAACCTGCGGCTACCTTCTCCTATTGTGGTTGTTTAACACAGATTaaa > 1:625311/1‑225 (MQ=255) tCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATtgat < 2:590174/175‑1 (MQ=255) tGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAAGGTCGCATCTGGTTCTCTTCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTTTGGATGCTTTACAATGATTAAAAACGCTTcc > 1:476677/1‑227 (MQ=255) gCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCCCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCgc > 2:95304/1‑228 (MQ=255) aGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGGGGGAAGTCATCATATCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCCCATCAGGTTCTCATCATCGCGTAATCTTGATGAAACCTGCGGCATCCTTCTTCTCTTTCGGATGCTTTTCCATGATATAAAAAGCTTCTTTGCTGTCGGCGGGTTTCGTCAAGGCATTTTcac > 1:425803/1‑226 (MQ=255) gtggtttGGGTTTTGCGGAACGTGCCGTTCGGTGGGAAGCCATCATCTCTTAGTATATTAGATCCCCCGCCAAGCCCGAAGACCGGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCGTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTggg < 1:60948/223‑1 (MQ=255) gtttgacttgttgtgatacggCCATTCGGTGGAAAGTCTTCACCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGca < 2:676682/207‑1 (MQ=255) gatTGCGGTATGCGGACCCTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGca < 2:499035/227‑1 (MQ=255) gatGGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATCCCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGca < 2:476677/227‑1 (MQ=255) atTGGGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTTTTAGTAACTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGca < 2:675562/226‑1 (MQ=255) ttGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATTTCTTAGTATCTTAGATACCCCTTCAAGGCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGcaca < 2:425803/227‑1 (MQ=255) ttttcggaacgtGCCATTCGGTGGGAAGTCCTCACCTCTTAGTAACTTGGATCCCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATAc < 1:5860/216‑1 (MQ=255) tGCGGACCCTGCCATTTGGTGGGAAGTCCACATCTCTTAGTATCTTAGATTCCCCTCCAAGCCCCAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAg < 2:685681/228‑1 (MQ=255) cccgtcccttCGGTGGGAAGTCATCACCTCCTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCCCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGa < 1:343292/220‑1 (MQ=255) gcTGCCATTCGGTGGGAAGTTATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCGTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGa < 2:178542/226‑1 (MQ=255) ttCGGTGGGCAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCtcatc > 1:597839/1‑87 (MQ=255) tCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCtcatca < 2:597839/87‑1 (MQ=255) tGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCACGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAAGATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGTTGTCGTCGCGTTCCGTGAAGGCGTTTTCCGCTTCGTCACCAAATACCAACCCGGATaaagatgtcg > 2:264981/1‑217 (MQ=255) atcatcTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGAGACTCTCGACGTTACTCCTacc > 2:430589/1‑226 (MQ=255) aGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCCCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGTCGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCAAAGGCAACCAACCCTGTTACCCTCGTCGTTCCTGACAACCGTTTTGAAc > 1:689338/1‑228 (MQ=255) aGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAAAGTCGCATCTGGTTCTCATCATCGCGTCATATTGATGTAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGCCGGCGTGTTCCGTCACGGCCTTTTCCGCTTGGCCATAGGTTACCAGCCCGGCTATTCTCGCCGTTCATGCTGCCCGTTTTGaa > 2:471596/1‑227 (MQ=255) tagttttgATTCCCCCCAAACCCCGAAGACCTGCCGGCCAACGTAGCATCTGGTTCTCATCAGCGTGTAATATTGATGAAACCTGCGGCCTCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAg < 1:235706/221‑1 (MQ=255) tATCTTAGATCCCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAg < 2:355574/228‑1 (MQ=255) cTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACCCTCGCCGTTTCTGCTCACCGTTTTGAACAGCcg > 2:390217/1‑228 (MQ=255) tgattcccccTCCAAGCCGCAAACCCTGCCGGCCAACGTCGCCTCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCcgcg < 2:625311/222‑1 (MQ=255) tAGATCCCCGTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCAACTGGTTCTCATCATCGCGTAATAATGATGAAACCTGCGGCATCCTGCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCGCGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCcgcg < 2:658672/228‑1 (MQ=255) tACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGCTACCACCCGGGATACTCTCGTCGTTACTGCTAACCGGTTTGAACAAccagccacc > 1:184398/1‑221 (MQ=255) aCCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCCCTGCTGACTGCGTGTTCCGTCACGGCTTTTTCCCATTGGTCACAGGTGACCACCCCGGCTACTCTCGCCTTTACTGCTAACCCTTTTGAAAACCAGCgcacaa > 1:339526/1‑225 (MQ=255) ccccccccAGCCCGAAACCCTGCCGGCCAACGTCGCATTTGGTTCTCATTATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCAc < 1:95304/228‑1 (MQ=255) ccccccCAAGCCCGAAGACCTGCCGGCCAACCGCGCAACTGGTTCTCATCATCGCGTAATTATGATGAAACCTGCGGCAACCTTCTTCGATTGTGGATGCTTTACAATGATTAAAAAAGCGTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCAc < 2:302261/228‑1 (MQ=255) ccccTTCACTCCCCAAGACCCTGCGGGCAACGGCGCGTCTGGTTCTCAACATCGCTTAATATTGATGAAACCTGCGGCTTCCTTCATCTAGTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCAc < 1:438821/228‑1 (MQ=255) ccccTCCCACCCCCAAGACCTGCCGGCCCACGTCGCATCTGGTTCTCATCGTCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCAc < 1:556836/228‑1 (MQ=255) ccccccAAGCCCGAAGGCCTGCCGGCCAACGTTGCATCTGGGTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCAc < 2:223811/227‑1 (MQ=255) cccTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACCGCTAACCGGTTTGAACAGCCGCGCCGCCCt > 2:28470/1‑228 (MQ=255) cccTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTCCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCCtttctcttataaacatctccg > 2:6059/1‑209 (MQ=255) cccTCCAAGCCCGAAGACCTGCCGGCCAAAGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCCGCATCCTTCTTCTATTGTGGCTGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGAGTTTTCCGCTTGGGCTCAGGATACCAGTCCGTATACACCCGCGGTTACTCCTACACGGTTTGAAAACCCGCgcacaacg > 1:225/1‑223 (MQ=255) cTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGa < 2:420267/180‑1 (MQ=255) cTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGa > 1:420267/1‑180 (MQ=255) cTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGGGTTCCGTCACGGCATTTTCCGCTTGGGCCCAGGATACCAGCCCGGATACTACCGTCGTTCCGGCTAACCGTTTTGAACAGCCGCGCCGCAcct > 2:235706/1‑225 (MQ=255) cTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTCACGGCGTGTTCCGGTACGGCATTTTCCGCTTGGGCACAGGAACACAGCCCGGTAACTTCCCTCCGTCATTCTACCCGTTTTGAAAAGCCGCccagcaacag > 1:660148/1‑224 (MQ=255) aGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGCTGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATAATCTCGCCGTTACTGCTAACCGTTTTGAACAGCCGCGCCGCACTTGGCTTg > 2:640227/1‑228 (MQ=255) aGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTACTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATCAAAAAAGCTTCGCTGCTGACGGCGCGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAACCCgg > 2:589630/1‑174 (MQ=255) aGCCCGAAGACCTGACGGCAAACGTCGCATCTGGTTCCCATCATCGCATAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACTGTGCTTg < 2:689338/228‑1 (MQ=255) ccGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGCAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAACAACCTTCGCTGCTGTCGGCTTGTTCCGTCACGGCATTTTCCGCTTTTGCACAGGATACCAGCCCCGACACTCTCGACGTTACTGCTCACCGTTTTTAACCGCCGCCGGGAACTGTGGTTgacc > 1:564793/1‑225 (MQ=255) ccGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGGGCAGCACTGGCCTTGccc > 2:438550/1‑226 (MQ=255) ccGAAAACCGTCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACTGTGCTTGCa < 1:437923/227‑1 (MQ=255) gAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAACCGCGCAGCACTAGGCTTGCacca > 1:84131/1‑228 (MQ=255) aaGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGCTACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCCCTGTGCTTGc > 2:437923/1‑223 (MQ=255) ggccaacgcgccacttgtttCTCATCCTCGCGTAATATTGATGAAACCTGCCGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACTGTGCTTGCACCAACCACCGTtgt < 1:169564/210‑1 (MQ=255) cggcgtttttGGTTTTCATCTTCGAGTAATTCTGATGAAACCTGCGGCATCCTTTTTCTATGGTGGATGCTTTACAATGATTAAAAAAGCTTCGGTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACTGTGCTTGCACCAACCACCGTTGTGAcc < 1:264981/217‑1 (MQ=255) | CTACGTCTGGACAGTAACTTGTTACAACCTGTAGCATCCACTTGCCGGTCCTGTGAGTTAATAGGGAATCCAGTGCGAATCTGGAGCTGACGCGCAGCGGTAAGGAAAGGTGCGATGATTGCGTTATGCGGACACTGCCATTCGGTGGGAAGTCATCATCTCTTAGTATCTTAGATACCCCTCCAAGCCCGAAGACCTGCCGGCCAACGTCGCATCTGGTTCTCATCATCGCGTAATATTGATGAAACCTGCGGCATCCTTCTTCTATTGTGGATGCTTTACAATGATTAAAAAAGCTTCGCTGCTGACGGCGTGTTCCGTCACGGCATTTTCCGCTTGGGCACAGGATACCAGCCCGGATACTCTCGTCGTTACTGCTAACCGTTTTGAACAGCCGCGCAGCACTGTGCTTGCACCAACCACCGTTGTGACC > NC_000913/4163355‑4163787 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |