BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,417,410:1	+C	3.9%	coding (458/750 nt)	yjfP →	acyl CoA esterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,417,408	1	.	C	3.9%	406.6 / 7.9	103	coding (456/750 nt)	yjfP	acyl CoA esterase
Reads supporting (aligned to +/- strand): ref base . (53/46); new base C (2/2); total (55/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

Only 100 of 104 total aligned reads displayed.

GCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGC‑GTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCG‑CCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTG‑CTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGGCGTGCGTCA  >  NC_000913/4417174‑4417644

gcggcgGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGC‑GTCGATGGGCGCGATGACGGCACTGGGGGTTACCGCTCG‑CCACCCCACGGGGGGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATACCTCCCCCGTTCACTGTT‑TCcc‑cccc                                                                                                                                                                                                                                            >  2:202847/1‑237 (MQ=255)

  ggcggTTAAATCATTTTTGGAAAATTTTGCTACAAAGTAGGGAGTAATTCACTCCTTTCGGGGCGGTAAGAGGGGAAGAAAACGGGCTGTTGGATGACGGTCGGGCAGTGGGTGGCGC‑GTCGATGGGCGAGATGACGGCACTGGGGATTACCGCTCG‑CAACCCCACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCt                                                                                                                                                                                                                                           <  1:731967/238‑1 (MQ=255)

                ttCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTCCTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGC‑GTCGATGGGCGCGATGACGGCACTGCGGATTACCGCTCG‑CCACCCACCGGTGGGCTGTACCGCCAGCATGAGGGGATC‑GGGCTATTTTACATCACTCGCCCGTTACCTGCT‑TCCA‑CCGCAGATACCTGAAACGgc                                                                                                                                                                                                                            >  1:525820/1‑239 (MQ=255)

                ttCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGC‑GTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCG‑CCACCCCACGGTGCGATGAACCGCCAGCATGATGGGACC‑GGGCTATTTTCCACCACTCCCCCGTTCACTGTT‑TCAC‑CCGccgttccactaaacgac                                                                                                                                                                                                                            >  1:200301/1‑223 (MQ=255)

                 tCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGC‑GTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCG‑CCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACgga                                                                                                                                                                                                                            >  2:264239/1‑237 (MQ=255)

                    ggaaaaTTTTTCTCCAAAGTATGCAGGAATTCACTACTTAACGGGCGGCAATAGCCGAAGAAATCTGGCTGCTGGAGGGCCGTCTTGAAGGCGGTGGCGC‑GTCGATGGGCGCGATGACGGCACTGGGGATTCCCGCTCG‑CAACCCCACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGgcagca                                                                                                                                                                                                                        <  1:264239/236‑1 (MQ=255)

                                gaaaaaGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGC‑GTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCG‑CCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAgaatgaa                                                                                                                                                                                                            <  2:618248/236‑1 (MQ=255)

                                                attccatactTTACGGGCGGAAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGC‑GTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCG‑CCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTc                                                                                                                                                                                             <  2:969884/232‑1 (MQ=255)

                                                 ttCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGC‑GTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCG‑CCCCCCCACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTCCATCCCTCGCCCGTTCACTGTT‑TCCA‑CCGCTGAACCCTGAAACGGCAACACAGCAGAAAGAATTCAATAAAATTGTcgc                                                                                                                                                                                           >  1:312323/1‑239 (MQ=255)

                                                 ttCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGC‑GTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCG‑CCACCCCACGGTGAGATGTACCGCCAGCCTGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACACCAGAATAAATTCAAAAAAATTGTcgg                                                                                                                                                                                           >  1:860318/1‑238 (MQ=255)

                                                 ttCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGC‑GTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCG‑CCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCCCTGTT‑TCCA‑CCGCTGATACCTGAAACGGCACCACAGCAGAATGAATTCAATAACATTGTcg                                                                                                                                                                                            >  2:809229/1‑238 (MQ=255)

                                                 ttCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGC‑GTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCG‑CCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTAAAAAACaattacgc                                                                                                                                                                                           >  1:431490/1‑232 (MQ=255)

                                                 ttCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGC‑GTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCG‑CCACCCCACGGGGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGATCACTGTT‑TCCA‑CCGCTGTGACCTTAAACGGCACCCCATCGAAATAAATACAA‑AACAATGTcag                                                                                                                                                                                           >  1:402046/1‑236 (MQ=255)

                                                 ttCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGC‑GTCGATGGGCGCGATGACGGCACTGGGGATTACCCCTGG‑CCACCCCCCGGTGAGATGTCCCGCCAGCATGATGGGACC‑GGGCTATTTTACATCTATCCCCCGTTAACTTTT‑TCCC‑CCGCTGATACCTGAAACTGCAGCAACCCAAAATGAATTCAAAAAAAtttgccc                                                                                                                                                                                           >  1:774723/1‑234 (MQ=255)

                                                     cttctTTACGTGCGGCAATAGCCGAAGAAACCTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGC‑GTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCG‑CCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCa                                                                                                                                                                                       <  2:860318/236‑1 (MQ=255)

                                                          tttgggggggCAATAGCCGAAAAAAACTGGCTGGTTGATGACCGGCTGGCAGGCGGGGCCGC‑GTCGAGGGGCGCGATGACGGCACTGGGGATTACCGCTCG‑CCCCCCCACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGc                                                                                                                                                                                  <  1:760261/231‑1 (MQ=255)

                                                           tACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGC‑GTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCG‑CCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATCGGGGCTATTTTACATCACTCGCCCGTCCCATGTT‑TCCA‑CCCCTGTTCCCTGAAACGGCAGCACAGCAGAATGAATTCAaaaaaacttcaggcccactggc                                                                                                                                                                                  >  1:462702/1‑221 (MQ=255)

                                                           tACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGC‑GTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCG‑CCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTAACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCCCAGCCGAATGAATTCAATAACATTGTCGCGCCACTGGCa                                                                                                                                                                                 >  2:76167/1‑239 (MQ=255)

                                                                  ggCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGC‑GTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCG‑CCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGgggg                                                                                                                                                                          >  2:534258/1‑239 (MQ=255)

                                                                  ggCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGC‑GTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCG‑CCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTggg                                                                                                                                                                          >  2:244559/1‑239 (MQ=255)

                                                                  ggCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGC‑GTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCG‑CCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTggg                                                                                                                                                                          >  2:760261/1‑239 (MQ=255)

                                                                  ggCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGC‑GTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCG‑CCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCAACTGGCAGAGTggg                                                                                                                                                                          >  2:148829/1‑239 (MQ=255)

                                                                   gCAATAGCCGAAGAAAACTGGCTGCTTGATGCCCGTCGGGCAGTCGGTGGGGC‑GTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCG‑CCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTggg                                                                                                                                                                          <  2:236381/238‑1 (MQ=255)

                                                                            gaagaaAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGC‑GTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCG‑CCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACaa                                                                                                                                                                >  2:78546/1‑238 (MQ=255)

                                                                            gaagaaAACTGGCTGCTTGAGGACCGTCTGGCAGTCGGTGGCGC‑GTCGATGGGCGGGATGACGGCACTGGGGATTACCGCTCG‑CCCCCCCACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACaaa                                                                                                                                                               <  2:431490/239‑1 (MQ=255)

                                                                             aagaaacgtggttgtttgtgacccGTCTGGCAGGCGGTGGCGC‑GACGATGGGCGAGATGGCGGCACTGGGGATTACCGCTCG‑CCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACaaa                                                                                                                                                               <  2:297085/216‑1 (MQ=255)

                                                                                       gctgctTGATGACCGTCTGGCAGTCGGTGGCGC‑GTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCG‑CCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACCGCAGAATGAATTCAATAACATTTTCGCGCCCCTGGCAGAGTGGG‑AGGGGACAAACCCACTGAaac                                                                                                                                                    >  2:902472/1‑239 (MQ=255)

                                                                                            ttGATGCCCGTCTGGCAGTCGGTGGCGC‑GTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCG‑CCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACt                                                                                                                                                <  2:525820/238‑1 (MQ=255)

                                                                                                 gcccGTCTGGC‑GTCTGGGGGGCGGTCGATGGGCGCGATGACGGCACGGGGGATTACCGCTCG‑CCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGa                                                                                                                                          <  2:109897/237‑1 (MQ=255)

                                                                                                  gcccgttcggagggcggggcggggTCGGTGGGCGCGATGACGGCACTGGGGATTTCCGCTCG‑CCACCCCCGGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGa                                                                                                                                          <  2:877255/216‑1 (MQ=255)

                                                                                                  cccGTTTGGCCGTGGGGGGGGG‑GTGGAGGGGGGGGGTGCCGGCCCTGGGGTTTCCCGCTCC‑CCCCCCCACGGTGAGATGTCCCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCCCTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGAc                                                                                                                                         <  1:1008847/238‑1 (MQ=255)

                                                                                                   ccGTCGGGCAGGCGGGGGGGG‑GTCGATGGGCGCGATGACGGCACTGGGGTTTCCCGCTCG‑CCACCCCCCGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACa                                                                                                                                        <  2:979272/239‑1 (MQ=255)

                                                                                                    cGTCTGGGAGTCGGTGGCGC‑GTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCG‑CCACCCCACGGTGAGATGTTCCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAg                                                                                                                                       <  2:200301/239‑1 (MQ=255)

                                                                                                    cGTCGGGCAGCCGGGGGGGC‑GTCGAGGGGCGCGATGACGGCCCGGGGGATTACCGCTCG‑CCACCCCCCGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAg                                                                                                                                       <  2:312323/239‑1 (MQ=255)

                                                                                                      tCTGGCAGTCGGTGGCGC‑GTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCG‑CCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCCAACCAGCATGAATTAAATAACATTGTCGCGCCACTTGCGTGGTGGG‑AAGGGACAACACACCTGGAACACATAAGAGACAGa                                                                                                                                      >  1:456579/1‑238 (MQ=255)

                                                                                                      tCTGGCAGTCGGTGGCGC‑GTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCG‑CCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAgaatg                                                                                                                                                                                                              >  1:599465/1‑167 (MQ=255)

                                                                                                      tCTGGCAGTCGGTGGCGC‑GTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCG‑CCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAgaatg                                                                                                                                                                                                              <  2:599465/167‑1 (MQ=255)

                                                                                                      tCTGGCAGTCGGTGGCGC‑GTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCG‑CCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGGGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAGa                                                                                                                                      >  2:1008847/1‑238 (MQ=255)

                                                                                                      tCTGGCAGTCGGTGGCGC‑GTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCG‑CCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAGa                                                                                                                                      >  2:684534/1‑238 (MQ=255)

                                                                                                         ggCTGTCGGGGGCGC‑GTCGATGGGGGCGATGCCGGCACTGGGGCTTCCCCCTCG‑CCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAGACctc                                                                                                                                  <  1:244559/239‑1 (MQ=255)

                                                                                                              tCGGTGGCGC‑GTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCG‑CCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGACCGTTCACTGTT‑TCCA‑CCGCTGGTACCTGAAACGGCAGCACCGCAGAATGAATTCAATAACATTTTCCGCCCCCTGCCAGAGGGGGAAAGCG‑CCAACAACCTGGACAAACttgtataacaaacctccac                                                                                                                              >  1:589738/1‑221 (MQ=255)

                                                                                                                ggTGGCGC‑GTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCG‑CCACCCAACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTCTTTTATATCACTCGCCCGGTCACTGTT‑TCCA‑CCTCTGATACCCGAAACGGCAGCACAGCCGAATAAAATTAATAACATTTTATAGCCCATGCCCTAGAGGA‑AGAGGACAAACCACCTGGAACAACTTAggaccaacccactactgtt                                                                                                                           >  1:809657/1‑221 (MQ=255)

                                                                                                                         gTCGAGGGGCGCGATGCCGGCACGGGGGATTACCGCCCGCCCACCCCA‑GTGGAGATGTCCCGCCAGCATGATGGGATC‑GGGCTATTTTACACCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTG‑CTGTGGCAt                                                                                                                   <  2:894333/238‑1 (MQ=255)

                                                                                                                                 gcgcGATGACGGCCCGGGGGATTACCGCTCG‑CCACCCCACGGTGGGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTG‑CTGTGGCATGGCCTcga                                                                                                           <  2:774723/238‑1 (MQ=255)

                                                                                                                                 gcgagggtgcGGCCCGGGGGTTTCCCCCTCC‑CCCCCCCCGGGTGGGATGGTCCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACGGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTG‑CTGTGGCATGGCCTcga                                                                                                           <  1:148829/229‑1 (MQ=255)

                                                                                                                                  cgcgATGACGGCACTGGGGATTACCGCTCG‑CCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTG‑CTGTGGCATGGCCTcgacg                                                                                                         >  2:866195/1‑239 (MQ=255)

                                                                                                                                    cgttgccggccttGGGGTTTCCCGCTGC‑CCCCCCCCCGTGGAGGTGTACCGCCAGCATTATGGGATG‑GGGCTATTTTACATCCCTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTG‑CTGTGGCATGGCCTcgacga                                                                                                        <  1:953546/226‑1 (MQ=255)

                                                                                                                                    cgatgcgggccttGGGGATTCCCGCCCG‑CCCCCCCGCGGGGAGTTTTACCGCCAGCATGATGGGATC‑GGGCTATTTTACCTCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTG‑CTGTGGCATGGCCTcgacga                                                                                                        <  2:589738/226‑1 (MQ=255)

                                                                                                                                             cccTGGGGATTACCGCTCG‑CCCCCCCACGGGGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTG‑CTGTGGCATGGCCt                                                                                                              <  2:726519/221‑1 (MQ=255)

                                                                                                                                                      taagagaccG‑CCCCCCCGCGGTGGGATGTACCGCCAGCATGATGGGATC‑GGGCTAGTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTG‑CTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCcgac                                                                                     <  2:458852/231‑1 (MQ=255)

                                                                                                                                                       tACCGCTCG‑CCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACgg                                                                                                                                                                                                                             <  1:701200/104‑1 (MQ=255)

                                                                                                                                                       tACCGCTCG‑CCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACgg                                                                                                                                                                                                                             >  2:701200/1‑104 (MQ=255)

                                                                                                                                                       tACCGCTCG‑CCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCCCGCCACTGGCAGAGTGGG‑AAGCTACACACCACCTGGAACAACTTAGTAACAGACCTCGACTGCCTGT‑GCCTGGCCCCGACGCTGCACTCGTGACTGCccacg                                                                                    >  1:179851/1‑239 (MQ=255)

                                                                                                                                                          cGCTCG‑CCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACAACCTGGAACAACTTAGTGACAGACCTCTACTG‑CTGTGGCCTGGCCTCGCCGATGACGTTGTGCCTGCCGACGaa                                                                                  >  2:266750/1‑238 (MQ=255)

                                                                                                                                                            cTCG‑CCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTG‑CTGTGGCATGGCCTCGACGATGACGTTGGGCCTGCCGACGAATc                                                                                >  2:947850/1‑238 (MQ=255)

                                                                                                                                                              cG‑CCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGGGGGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGCCCGACCACCACTG‑CCGTGGCATGGCCCCGAACATGACGGTGTGCCGCCCGACaaaaccac                                                                             >  1:733477/1‑234 (MQ=255)

                                                                                                                                                              cG‑CCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTG‑CTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACt                                                                             >  2:869040/1‑239 (MQ=255)

                                                                                                                                                              cG‑CCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACAACCTGGAACAACTTAGTGACAGACATCTACTG‑CTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACt                                                                             >  2:449376/1‑239 (MQ=255)

                                                                                                                                                                ggtgtataagagaagaaTGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTG‑CTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACt                                                                             <  2:492462/222‑1 (MQ=255)

                                                                                                                                                                            tGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACAACCTGGAACAACTTAGTGAAAAACCGCTCCTG‑CGGTGGCACGGCCCCCACGCTGACGTTGTGCCTGCCGACaaacaacaacgcggccctcta                                                                >  1:458852/1‑221 (MQ=255)

                                                                                                                                                                            ggaggTGTACCGCCAGGTTGATGGTATC‑GGGTCTTTTTACAGCACTCCCCCGTTCACTGTT‑CCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGGCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTG‑CTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTgcagca                                                                 <  1:78546/237‑1 (MQ=255)

                                                                                                                                                                               gaTGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGa                                                                                                                                          >  2:714220/1‑163 (MQ=255)

                                                                                                                                                                               aaTGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGa                                                                                                                                          <  1:714220/162‑1 (MQ=255)

                                                                                                                                                                                    cccGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCCCTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTG‑CTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAg                                                        <  2:810372/238‑1 (MQ=255)

                                                                                                                                                                                    aCCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTg                                                                                                                                                        <  2:199422/144‑1 (MQ=255)

                                                                                                                                                                                    aCCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTg                                                                                                                                                        >  1:199422/1‑144 (MQ=255)

                                                                                                                                                                                    aCCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGaac                                                                                                                                                    <  2:848639/148‑1 (MQ=255)

                                                                                                                                                                                    aCCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGaac                                                                                                                                                    >  1:848639/1‑148 (MQ=255)

                                                                                                                                                                                      cGCCATCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTG‑CTGTGGCa                                                                                                                    >  1:321589/1‑177 (MQ=255)

                                                                                                                                                                                      cGCCATCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTG‑CTGTGGCa                                                                                                                    <  2:321589/177‑1 (MQ=255)

                                                                                                                                                                                         cAGAATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTG‑CTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCgaga                                                   <  2:746780/239‑1 (MQ=255)

                                                                                                                                                                                          aGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTG‑CTGGGGCATGGCCCCGACGAAGACGTTGTGGCTGCCGACGAAACACCCAGCTTGCAACAGGCCTAAAGGGAgac                                                  >  1:536241/1‑239 (MQ=255)

                                                                                                                                                                                           gCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGGGACAACCCTCTACTG‑CTGTGGCATGGCCCCGACGATGACGTTTTGCCTGCCGCCGAATCACTACGTTTGCAGCCGGctcctttaaaaaa                                                  >  1:492462/1‑226 (MQ=255)

                                                                                                                                                                                            cATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTAct                                                                                                                              >  2:147596/1‑162 (MQ=255)

                                                                                                                                                                                             attgtgGGATG‑GGGGTTTTTTACATCCCTCGCCCGTTCACTGTT‑TCCC‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTG‑CTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAgacggg                                               <  1:947850/235‑1 (MQ=255)

                                                                                                                                                                                                 tgGGTTC‑GGGTTTTTTTCCCTCCCCCGCCCTTTCACTTTT‑TCCA‑CCGCTGATACCGGAAACGGCAGCACAGCAGAATGAATTCAATAACAGTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTG‑CTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAgacgggacg                                            <  1:684534/238‑1 (MQ=255)

                                                                                                                                                                                                       tctggtttttttACATCCCCCCCCCTTTCACTGTT‑TCCC‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTG‑CTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATa                                       <  1:617026/230‑1 (MQ=255)

                                                                                                                                                                                                             ttttttACCTCACTCGCCCTTTTCCGTTT‑TCCC‑CCCGTGATACCGGAAACGGCAGCCCAGAAAAATGAATTCAATAACATGGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTG‑CTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCt                                <  1:202847/237‑1 (MQ=255)

                                                                                                                                                                                                               ttttACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑ACGCGACAAACCACCTGGAACAACTTAGTGACAGACATCTACTG‑CTGTGCCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCCCTCCGTTTGCAGCAGGCCTTAACTCACCCGGGGCGGGATAAACCGCaaa                              >  1:887611/1‑239 (MQ=255)

                                                                                                                                                                                                               ttttACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTG‑CTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTTCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTa                               >  2:606001/1‑238 (MQ=255)

                                                                                                                                                                                                                tttACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATCCCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTG‑CTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAAc                             <  2:462702/239‑1 (MQ=255)

                                                                                                                                                                                                                tttACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAaccacc                                                                                                                                                          >  2:1001795/1‑115 (MQ=255)

                                                                                                                                                                                                                tttACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAaccacc                                                                                                                                                          <  1:1001795/115‑1 (MQ=255)

                                                                                                                                                                                                                tttACACCACGCGCCCGTTCCCTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTG‑CTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAAc                             <  2:536241/239‑1 (MQ=255)

                                                                                                                                                                                                                 ttccATCACTCGCCCGTTCACTGTT‑TCCACCCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGGCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCCCCTGGAACAACTTAGTGACAGACCTCTACTG‑CTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAAc                             <  2:982045/236‑1 (MQ=255)

                                                                                                                                                                                                                 ttACATCACTCGCCCGTTCACTGTT‑TCCACCCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGGCGCGGCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTG‑CTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAAc                             <  2:13961/239‑1 (MQ=255)

                                                                                                                                                                                                                    cATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTG‑CTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGGGAAACGGCTAACCTGt                         >  2:377952/1‑239 (MQ=255)

                                                                                                                                                                                                                     aTCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACCGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAGCCCCATACTG‑CTGTGCCATGACCGCGACGCTGCCGTTGTGCCTGCCGACCAAACCCCCCTGTTGCCGCACGCCTTAAACGGACCGGGAGGGGGTAAAATCCTAACCCGtt                        >  1:622597/1‑239 (MQ=255)

                                                                                                                                                                                                                     aTCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTG‑CTGTGGCATGGCCTCGACGATGCCGTTGTGCCTGCCGACGAATCACTACGTTTTCAGCAGGCCTTAAGCGAGACGGGACGGGAAAAACGCTTAACCTGtt                        >  1:318666/1‑239 (MQ=255)

                                                                                                                                                                                                                         cccGCCCGTTCCCTTTTCTCCA‑‑CGATGTAACCAGAAACGGCAACACAGGTGAATGAAATTAATTACTTTGTCGCGCCACTGGCAGAGTGGG‑AAGCATCAAACCTCCTGGAAATACTTAGGTACTGACCTCTACTG‑CTGTGGCATTGCCTCGACGATGGCGTTGTGCCTGCCGACGAAGCACTTCGTTTGCTGCAGGCCTTAAGCGAGGCGGGACGGGATAAACTGCTATCCTGTTCATg                    <  1:809229/237‑1 (MQ=255)

                                                                                                                                                                                                                         cTCGCCCGTTCACTGTT‑TCCACCCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACACACCACCTGGAACAACTTAGTGACAGACCTCTACTG‑CTGTGGCATGGCCTCGACGATGACGTTGTCCCTGCCGACGAAACCCTAAGGTTGCAACAGTCCTTAAGCGAGCCGGGACGGGATAAACTGGCAACCTGTTCAt                     >  1:982045/1‑239 (MQ=255)

                                                                                                                                                                                                                         cTCGCCCGTTCACTGTT‑TCCACCCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTG‑CTGTGGCATGGCCTCGACGATGAGGTTTTGCCTGCCGCCCAATCCTTCAGTTTGCCACCGGCCTTAAGGGCGACGGGCGGGGGAAAAATGCTAACCtagtagt                     >  1:13961/1‑233 (MQ=255)

                                                                                                                                                                                                                         cTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAaccacc                                                                                                                                                          >  2:243573/1‑106 (MQ=255)

                                                                                                                                                                                                                         cTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAaccacc                                                                                                                                                          <  1:243573/106‑1 (MQ=255)

                                                                                                                                                                                                                         cTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTG‑CTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCTGTTCATg                    <  2:402046/239‑1 (MQ=255)

                                                                                                                                                                                                                                 ttCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTG‑CTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCTGTTCATGCCAGcagc            >  2:802006/1‑236 (MQ=255)

                                                                                                                                                                                                                                   cACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTG‑CTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGGGACGGGACGGGATAAACTGCTAACCTGTTCATGGAAGCCCGgc          >  2:518805/1‑239 (MQ=255)

                                                                                                                                                                                                                                        tt‑tCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTG‑CTGTGGCCTGGCCTCGACGATGACGTTTTGCCTGCCGACCAATCACTACGTTTGCCGCAGGCCTTAAGCGAGACGGGACGGGACAAACCGCTACCCCGGCCCTGGGAGCAAGgcgtgcg     >  1:638301/1‑239 (MQ=255)

                                                                                                                                                                                                                                         t‑tCCA‑CCGCTGATACCTGAAACGGGAGCCCAGGAGAAAGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGAAAAACCACCTGGAACAACTTAGTGACAGACCTCTACTG‑CTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGgcgtgcg     <  2:622597/238‑1 (MQ=255)

                                                                                                                                                                                                                                            ccA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTG‑CTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCACCAGGCCTTAAGCGGGACGGGACGGGATAAACTGCTAACCCGTTAAGGGAAGCCAGGGGGGCGGCa  >  1:124969/1‑239 (MQ=255)

GCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGC‑GTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCG‑CCACCCCACGGTGAGATGTACCGCCAGCATGATGGGATC‑GGGCTATTTTACATCACTCGCCCGTTCACTGTT‑TCCA‑CCGCTGATACCTGAAACGGCAGCACAGCAGAATGAATTCAATAACATTGTCGCGCCACTGGCAGAGTGGG‑AAGCGACAAACCACCTGGAACAACTTAGTGACAGACCTCTACTG‑CTGTGGCATGGCCTCGACGATGACGTTGTGCCTGCCGACGAATCACTACGTTTGCAGCAGGCCTTAAGCGAGACGGGACGGGATAAACTGCTAACCTGTTCATGGCAGCCAGGCGTGCGTCA  >  NC_000913/4417174‑4417644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`