Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	NC_000913	3,984,188	(AGTA)2→1	2.9%	coding (1232‑1235/1236 nt)	aslB →	putative AslA‑specific sulfatase‑maturating enzyme

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3984187	102 (1.300)	3 (0.040)	3/402	NT	2.9%	coding (1231/1236 nt)	aslB	putative AslA‑specific sulfatase‑maturating enzyme
	?	NC_000913	3984192 =	NA (NA)					coding (1236/1236 nt)	aslB	putative AslA‑specific sulfatase‑maturating enzyme

AAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAG‑TA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3984113‑3984187 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ag‑taGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGCAAAGGCAGATCTCAGCGATAGCGCCGGCTTAGTCAGATTTAATCTGCGCGCGTGGTGGATATTTTTTCAGGATCTCCATATACGCGTGCATTTCGGTCTGTAGCGGTA  >  NC_000913/3984192‑3984430                                                                                                                                                                                                                                                                                                                             aagtattggttttTTTGTTGGCGCGCGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAG‑TAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGCAAAGGCAGATCTCAGCGATAGCGCCGGCTTAGT                                                                             <  1:127083/226‑1       TGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAG‑TAGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGCAAAGGCAGATCTCAGCGATAGCGCCGGCTTAGTCAGATT                                                                       >  2:127083/1‑239                                          agagccgttccgattttgTTGGTGGTGGTTAGTTAAAAGTCGGCGGCCGGCTGCGGTTGATTGCCGGATGCGGCGTAAACGTCTTATCCGGCCTACATGCTCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGCAAAGGCAGATCTCAGCGATAGCGCCGGCTTAGTCAGATTTAATCTGCGCGCGTGGTGGATATTTTTTCAGGAT                                     <  1:586700/221‑1                                                                            AGAAATCGGCGGCCTCCTGCGGTTGATGGCCGGATGC‑GCGTAAACGCCTTATCCGGCCTAAATGATCGGGCAAATTCAATAAATTGCAGCGTTCTGGAGGCTGGATAAGATGCGTCAGCATCGCATCCGGCAAAGGCAGATCTCAGCGATAGCGCCGGCTTAGTCAGATTTAATCTGCGCGCGTGGTGGATATTTTTTCAGGATCTCCATATACGCGTGCATTTCGGTCTGTAGCGGTA  <  2:957883/239‑1                                                                                                                                                                                                                                                                                                                             AAGCAATGGCTGATTTGCTGGCGCACGGTCGCCCGGCCAGCGACATTATGCATGCGCATTTGCTGGTGGTGAG‑TA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3984113‑3984187 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ag‑taGAAATCGGCGGCCGCCTGCGGTTGATTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACATGATCGTGCAAATTCAATAAATTGCAGCGTTCTGTAGGCTGGATAAGATGCGTCAGCATCGCATCCGGCAAAGGCAGATCTCAGCGATAGCGCCGGCTTAGTCAGATTTAATCTGCGCGCGTGGTGGATATTTTTTCAGGATCTCCATATACGCGTGCATTTCGGTCTGTAGCGGTA  >  NC_000913/3984192‑3984430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.