New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1979489	0 (0.000)	57 (0.720)	39/410	NT	100%	intergenic (‑274/‑264)	insA/uspC	IS1 repressor TnpA/universal stress protein
	?	NC_000913	= 2290111	NA (NA)					intergenic (‑32/+1)	insH1/yejO	IS5 transposase and trans‑activator/pseudogene, autotransporter outer membrane homology,putative transport, Not classified, putative ATP‑binding component of a transport system

AGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGAAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTTACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTG  >  NC_000913/1979251‑1979727                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                               aaaacgataaccaactggcgatgttattcacgctggccaacctgtttcgggcggaccaaatgatacgtcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTaa                                                                                                                                                                                                                                                >  1:513453‑M1/236‑239 (MQ=255)       ataaccaactggcgatgttaatcacgctggccaacctgtttcgggcggaccaaatgatacgtcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAATTAAAt                                                                                                                                                                                                                                          >  2:367300‑M1/230‑239 (MQ=255)          caattacttaacataaatgtataAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggggc                                                                                                                                                                                                                                       >  2:337093‑M1/12‑230 (MQ=255)               ctggcgtttttattcccgctggccaacctgtttcgggcggaccaaatgatacgtcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAATTAAATACaaagaa                                                                                                                                                                                                                                  >  1:1013690‑M1/222‑239 (MQ=255)                         ttttcacgcttgcccacctttttcgggcggtacaaatgagtcgtcagtgggatagatctcactaaagactggggataacggcttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAATTAAATACAAAGAAAGGCAACAAt                                                                                                                                                                                                                        >  1:139888‑M1/212‑239 (MQ=255)                         tattcccgttgtcctacctgttgcgggtggaccaaatgattcttctgtgggatagatcgcactaaaaactgggtataacgccttaaatggcgaagaaacggtctaaataggatgaatcaaagcatgtacgggagaaaaactcggcacaaacatgaagaaatgaaatgactgagtcagccgagaagaatgcccccgcttatacgcacctaccCTAATTAAATACAAAGAAAGGCAACAAt                                                                                                                                                                                                                        >  1:294004‑M1/212‑239 (MQ=255)                         tattcacgctggccaacctgtttcgggcggaccaaatgatacgtcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAATTAAATACAAAGAAAGGCAACAAt                                                                                                                                                                                                                        >  2:955576‑M1/212‑239 (MQ=255)                         tattcacgctggcacacctgttgcggggggaccaaatgatacggcagtgggagagatctcactaaaaacgggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAATTAAATACAAAGAAAGGCAACAAt                                                                                                                                                                                                                        >  1:57411‑M1/212‑239 (MQ=255)                         atttttttttaaagagcaggtttcgggcggaccaaattgtacgtcagggggaaaaatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAATTAAATACAAAGAAAGGCAACAAt                                                                                                                                                                                                                        >  1:329393‑M1/212‑239 (MQ=255)                          aTGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgata                                                                                                                                                                                                                       >  2:543028‑M1/1‑214 (MQ=255)                            tcacgctggccaacctgtttctggcggaccaaatgatacgtcagtgggagagatctcactaaaaactggggctaacaccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggatcaaacatgaagaaatgaaatgactgagtcggacgagaagaatttccccggttattagcacattccctaattaaataactgtcccttatacacagct                                                                                                                                                                                                                     <  1:121544‑M1/31‑21 (MQ=255)                                gctggccaacctgtttcgggcggaccaaatgatacgtcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAATTAAATACAAAGAAAGGCAAAAATCAACtta                                                                                                                                                                                                                 <  2:139888‑M1/35‑1 (MQ=255)                                     caaacctgtttcgggcggaccaaatgatacgtcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAATTAAATACAAAGAAAGGCAACAATCAACttaatgt                                                                                                                                                                                                             >  2:79837‑M1/200‑238 (MQ=255)                                     aTACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCCCAAAAAACTAAggaaggtgcgaaaaaacccctgatataaaaccaatt                                                                                                                                                                                                            >  1:646750‑M1/1‑203 (MQ=255)                                      tACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAAATAAGCTATTTTTAAACAGACACTTACCGCAAAACCAACTAAgaaaagcgcaaacaaatcactgaaataataaaatgtt                                                                                                                                                                                                           >  1:279840‑M1/1‑202 (MQ=255)                                           caagtgtgtaaaagaaacaagttcggcagttggagagaactcaataaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAATTAAATACAAAGAAAGGCAACAATCAACTTAATGTTAATGaa                                                                                                                                                                                                      >  1:527449‑M1/194‑239 (MQ=255)                                             gttttgGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatataggatcaagtttgccttc                                                                                                                                                                                                    >  2:798387‑M1/1‑195 (MQ=255)                                                     ggacaaaatgttacgtcagtgggagagatctcactaaaaacgggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAATTAAATACAAAGAAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTAc                                                                                                                                                                                             >  1:134333‑M1/184‑238 (MQ=255)                                                      ttaTTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagcca                                                                                                                                                                                            <  2:245963‑M1/237‑53 (MQ=255)                                                       accaaatgatacgtcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAATTAAATACAAAGAAAGGCAACAATCAACttaatg                                                                                                                                                                                                              <  2:329393‑M1/38‑1 (MQ=255)                                                       accaaatgatacgtcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAATTAAATACAAAGAAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACata                                                                                                                                                                                          <  2:134333‑M1/58‑1 (MQ=255)                                                          aaatgatacgtcagtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAAt                                                                                                                                                                                                                                               >  2:116615‑M1/179‑183 (MQ=255)                                                              aTCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtcc                                                                                                                                                                                                                             <  2:204472‑M1/197‑20 (MQ=255)                                                              aTCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAAAAGACACTTACCGCACAAAAAACTAAggaaggtgcgaacaaatccctgatatgaaatcatgttagtaattggaggccaaaaaaaaag                                                                                                                                                                                   >  1:1022986‑M1/1‑178 (MQ=255)                                                              aTCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATAATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtcc                                                                                                                                                                                                                             >  1:204472‑M1/1‑178 (MQ=255)                                                               tCTGGTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacaggg                                                                                                                                                                                  <  2:880788‑M1/239‑63 (MQ=255)                                                                 cctacggtgggaaggtatttattaaaaactggggataacggcttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAATTAAATACAAAGAAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCAc                                                                                                                                                                                >  1:486494‑M1/172‑239 (MQ=255)                                                                   ttAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttca                                                                                                                                                                              >  2:839479‑M1/1‑173 (MQ=255)                                                                   ttAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttc                                                                                                                                                                               >  2:592489‑M1/1‑173 (MQ=255)                                                                   ttAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatca                                                                                                                                                                                                               <  1:459785‑M1/206‑34 (MQ=255)                                                                   ttAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatca                                                                                                                                                                                                               >  2:459785‑M1/1‑173 (MQ=255)                                                                   ttAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAgga                                                                                                                                                                                                                                             <  2:416658‑M1/176‑4 (MQ=255)                                                                   ttAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAATCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAgga                                                                                                                                                                                                                                             >  1:416658‑M1/1‑173 (MQ=255)                                                                      agtgggagagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAATTAAATACAAAGAAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGtt                                                                                                                                                                           <  2:434100‑M1/73‑1 (MQ=255)                                                                          ggagagatctcactaaaaactggggataacgccttaaatggcgaggaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtaagccgagaagaatttccccgcttattcgacccttccCTAATTAAATACAAGGAAAGGCAACAatcaatttattgttaatgaaaggttttcaatctgtcccttttacacatct                                                                                                                                                                        <  2:527449‑M1/76‑24 (MQ=255)                                                                             gagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAATTAAATACAAAGAAAGGCAACAATCAACt                                                                                                                                                                                                                   <  1:175706‑M1/33‑1 (MQ=255)                                                                             gagatctcactaaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAATTAAATACAAAGAAAGGCAACAATCAACt                                                                                                                                                                                                                   >  2:175706‑M1/160‑192 (MQ=255)                                                                               gatctcactaaaaacttgggtgaacacattaaagggagaaaaacatgtctaaatagactgattcaaggaagttacgggagaaaaaatcagcactaacaaaaagaaatgaaatgactgatgcagccgagaagaatttccccgcttattcgcaccttccCTAATTAAATACAAAGAAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCgg                                                                                                                                                                   >  1:742630‑M1/158‑238 (MQ=255)                                                                                atctccataaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAATTAAATACAAAGAAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGgag                                                                                                                                                                 >  2:795036‑M1/157‑239 (MQ=255)                                                                                        aaaaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAATTAAATACAAAGAAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTa                                                                                                                                                         >  2:223204‑M1/149‑239 (MQ=255)                                                                                          aaactggggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAATTAAATACAAAGAAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTaca                                                                                                                                                       >  2:66469‑M1/147‑239 (MQ=255)                                                                                                gggataacgccttaaatggcgaagaaacggtctaaataggctgattcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAATTAAATACAAATAAAGGc                                                                                                                                                                                                                              >  2:703416‑M1/141‑162 (MQ=255)                                                                                                        aaCAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaatt                                                                                                                                         <  2:1022986‑M1/239‑104 (MQ=255)                                                                                                                       aaTGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgcgatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttacctccgccgccagtgatttcagaagaaacgccc                                                                                                                           >  2:505335‑M1/1‑121 (MQ=255)                                                                                                                              tGTTGCGCACAGTCAAAATAACTCACCTGAAATAATCATCTGCTATAAATAATCACATTCATTCAATACCAGATAAGCTATTTTTAAACAGACTCTTACCGCACAACAAACTGAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgacagac                                                                                                                    <  1:767678‑M1/238‑125 (MQ=255)                                                                                                                                   cgcACAGTCAAAATAACTCACCGTAACTAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttaatcatgagtcatcaacttaccttcgccgacagtgaattcaccagtaagcgccgcaagaccagaaa                                                                                                              >  1:1000294‑M1/1‑109 (MQ=255)                                                                                                                                     cacaGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtcccagatatcagatcatgattgtcatctggagccatagaccagggttcatcatgagtcatcaacttaccttcgccgccagtgaattcagcagtaagcacatcaagacaagaaaaa                                                                                                            >  1:726380‑M1/1‑107 (MQ=255)                                                                                                                                        aGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgagaacaagtccctgatatgagatcatgtttgtcatcttgagccatagaaaaaggttcatcatgattcatcaacttaccttcgccgacagtgaattcagcagaaagcaccgtcagacaagaaaagaga                                                                                                         >  1:520528‑M1/1‑104 (MQ=255)                                                                                                                                        aGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACCTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaag                                                                                                                                                                                                                                <  2:981760‑M1/120‑17 (MQ=255)                                                                                                                                        aGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACCTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaag                                                                                                                                                                                                                                >  1:981760‑M1/1‑104 (MQ=255)                                                                                                                                            ttcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAATTAAATACAAAGAAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTaca                                                                                                                                                       >  2:454977‑M1/97‑189 (MQ=255)                                                                                                                                            ttcaaggcatttacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAATTAAATACAAAGAAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTaca                                                                                                                                                       <  1:454977‑M1/93‑1 (MQ=255)                                                                                                                                                  accccccgtaaaTAATTATTTGCTATAAATAATCCCTTTCATGCAAAACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaaggccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtc                                                                                               <  1:337093‑M1/234‑146 (MQ=255)                                                                                                                                                  aCTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtc                                                                                               <  2:726380‑M1/239‑146 (MQ=255)                                                                                                                                                  aCTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaag                                                                                                                                                                                                                                <  1:425833‑M1/110‑17 (MQ=255)                                                                                                                                                  aCTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaag                                                                                                                                                                                                                                >  2:425833‑M1/1‑94 (MQ=255)                                                                                                                                                    tCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccc                                                                                                                                                                                                                            >  2:66818‑M1/1‑92 (MQ=255)                                                                                                                                                    tCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccc                                                                                                                                                                                                                            <  1:66818‑M1/112‑21 (MQ=255)                                                                                                                                                       tacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAATTAAATACAAAGAAAGGcaacaa                                                                                                                                                                                                                         <  2:1320‑M1/27‑1 (MQ=255)                                                                                                                                                       tacgggagaaaaaatcggctcaaacatgaagaaatgaaatgactgagtcagccgagaagaatttccccgcttattcgcaccttccCTAATTAAATACAAAGAAAGGcaacaa                                                                                                                                                                                                                         >  1:1320‑M1/86‑112 (MQ=255)                                                                                                                                                        cGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcat                                                                                         >  2:682158‑M1/1‑88 (MQ=255)                                                                                                                                                        cGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagt                                                                                                                                              >  1:731946‑M1/1‑88 (MQ=255)                                                                                                                                                        cGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagt                                                                                                                                              <  2:731946‑M1/186‑99 (MQ=255)                                                                                                                                                            aataatCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccc                                                                                                                                                                                                                            <  1:608057‑M1/104‑21 (MQ=255)                                                                                                                                                            aataatCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccc                                                                                                                                                                                                                            >  2:608057‑M1/1‑84 (MQ=255)                                                                                                                                                            aaatatctatttctttaaaaaatccctttactgatatACCAGATAAGCTTTTGTGAAACAGACCCTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcaggagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggag                                                                                     <  1:592489‑M1/224‑156 (MQ=255)                                                                                                                                                                        cTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgcca                                                                         <  2:646750‑M1/239‑168 (MQ=255)                                                                                                                                                                        cTAAAAAAAATTCCTTTCAATCAAAACCAAATAAGCTATGTTTAAACGGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgggaccatgtttggcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgc                                                                           <  1:554310‑M1/237‑166 (MQ=255)                                                                                                                                                                                 atccccttcaaccaaatccagataagttatttttaaACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaac                                                                <  1:543028‑M1/222‑177 (MQ=255)                                                                                                                                                                                  tCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagcgattttcttgtcccgcatggagcagattctgccatggcaaaa                                                                 >  2:554310‑M1/1‑62 (MQ=255)                                                                                                                                                                                  tCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagccgattctgccatggcaaaaca                                                               >  2:375736‑M1/1‑62 (MQ=255)                                                                                                                                                                                  tCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagatttcattgtcccgcatggagcagattctgccatggcaaaaca                                                               >  2:701780‑M1/1‑62 (MQ=255)                                                                                                                                                                                       ttCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgcaatggcaaaacatggtg                                                          >  2:608233‑M1/1‑57 (MQ=255)                                                                                                                                                                                                tACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgccagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagccatc                                                 >  2:81745‑M1/1‑48 (MQ=255)                                                                                                                                                                                                tACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcttggagcagattctgccatggcaaaacatggtggaagtcatc                                                 >  2:233491‑M1/1‑48 (MQ=255)                                                                                                                                                                                                tACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaaactgggggaagtcatg                                                 >  2:154057‑M1/1‑48 (MQ=255)                                                                                                                                                                                                        aaGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaa                                                                 <  2:536483‑M1/215‑176 (MQ=255)                                                                                                                                                                                                        aaGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaa                                                                 >  1:536483‑M1/1‑40 (MQ=255)                                                                                                                                                                                                         agttttttttaaacaGCCCCTTACCGAACAACAAACTAAggaaggttcgaacaagtccctgatatgagatcatgtttgtcatcgggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttt                                        <  1:154057‑M1/234‑201 (MQ=255)                                                                                                                                                                                                         aGCTATTTTTAAACAGACCCTTACCGCACAACAAACTAAggaaggtgcgaacaagcccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttt                                        <  2:462389‑M1/239‑201 (MQ=255)                                                                                                                                                                                                         aGCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttt                                        <  2:479006‑M1/239‑201 (MQ=255)                                                                                                                                                                                                          gCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaccaagtccctgatatgagatcatgtttgtcatatgaagccatagaacagggttcatcatgcgtcatcaacttaccttcgccgacagggaattcagcagacagcgccgtcagaccagaaaagcgattttcttgtccccctcgaggcagattcacccatggcaaaacatagggtaaagcatcgaaccgtttt                                       >  1:273945‑M1/1‑38 (MQ=255)                                                                                                                                                                                                          gCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagcaatcgggccgttta                                       >  1:187676‑M1/1‑38 (MQ=255)                                                                                                                                                                                                          gCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggag                                                                                     >  1:1007208‑M1/1‑38 (MQ=255)                                                                                                                                                                                                          gCTATTTTTAAACAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggag                                                                                     <  2:1007208‑M1/193‑156 (MQ=255)                                                                                                                                                                                                                    aaCAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcat                                                                                         >  2:446463‑M1/1‑28 (MQ=255)                                                                                                                                                                                                                    aaCAGACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcat                                                                                         <  1:446463‑M1/179‑152 (MQ=255)                                                                                                                                                                                                                        gACACTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggt                         >  1:235216‑M1/1‑24 (MQ=255)                                                                                                                                                                                                                         acacTTACCGCACAACAAACTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggaa                        >  2:892261‑M1/1‑23 (MQ=255)                                                                                                                                                                                                                                          cacgaaggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccggcgaccttat       <  2:121949‑M1/235‑234 (MQ=255)                                                                                                                                                                                                                                           aCTAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccggcgaccttatc      <  2:187676‑M1/239‑235 (MQ=255)                                                                                                                                                                                                                                            cTAAggaaggtgccaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccggcgaccttatcc     <  2:368765‑M1/239‑236 (MQ=255)                                                                                                                                                                                                                                             tAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccggcgaccttatccg    <  2:90646‑M1/239‑237 (MQ=255)                                                                                                                                                                                                                                             tAAggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccggcgaccttatcc     >  2:512459‑M1/1‑3 (MQ=255)                                                                                                                                                                                                                                              aaggaaggtgcgaacaagtccctgatatgagatcatgtttgtcatctggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggcgcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccacggctgggaaaggccggcgaacatatccgg   >  1:90646‑M1/1‑2 (MQ=255)                                                                                                                                                                                                                                               aggagggtgcgaacaagtccctggtatgagatcatgtttgtcaactggagccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccggcgaccttatccgct  <  2:457449‑M1/239‑239 (MQ=255)                                                                                                                                                                                                                                               aggaaggtgcgaaaaagtctttggtttggtatcatgtttgtcacgtgggcccatagaacagggttcatcatgagtcatcaacttaccttcgccgacagtgaattcagcagtaagcgccgtcagaccagaaaagagattttcttgtcccgcatggagcagattctgccatggcaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccggcgcccttatccgct  <  1:174641‑M1/239‑239 (MQ=255)                                                                                                                                                                                                                                               aggaaggtgcaacacagtccttgattttaaatcatgtttgtcagctggagccatagaacagggttcataatgggccatcaacttcccttcgccgcctgtgaagtcagcagtaagcgccgtcagcccagaaaagagattttcttgtcccgcatggagcatattctgccatggaaaaacatggtggaagtcatcgagccgttttaccccaaggctggtaatggccggcgaccttatccgct  <  1:145609‑M1/239‑239 (MQ=255)                                                                                                                                                                                                                                               aTTAAATACAAAGAAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTTACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTg  >  2:218164/1‑239 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                               AGTAAGTTGGCAGCATCACCCATAAATGTATAAGTCATACTTTTGTTTTGGGTGTATTTCAATCTGTTAAAAAGTTTTTCGCTACGCTAGCAAGCAAAAATGAAACAGGAATAATCGAAATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGAAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGATTAATTATCTTTACTAATAATCAGACTAATATTTACCTGTTTGACCGAGTTGGGATTGCGTCGTTTCTCCATTAGGAGTAAAGCTTTAATGTCACCTG  >  NC_000913/1979251‑1979727

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.