Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC	NC_000913	3,399,169	Δ3 bp	6.3%	coding (806‑808/1104 nt)	mreC ←	cell wall structural complex MreBCD transmembrane component MreC

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3399168	59 (0.750)	4 (0.050)	3/410	NT	6.3%	coding (809/1104 nt)	mreC	cell wall structural complex MreBCD transmembrane component MreC
	?	NC_000913	3399172 =	60 (0.760)					coding (805/1104 nt)	mreC	cell wall structural complex MreBCD transmembrane component MreC

TGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTT‑GCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCG  >  NC_000913/3398934‑3399406                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                               tgtgGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTT‑GCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGGTCATTAGCAACACGATGCACCCCTTCCAGCGTCATCGGGTTAGCGCCGTTACAATCTGCCCCCCcca                                                                                                                                                                                                                                             >  1:948544‑M1/1‑235 (MQ=255) tggggAGGGCCATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTT‑GCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCAcag                                                                                                                                                                                                                                            <  2:320514/236‑1 (MQ=255)        agCGATTAGCCGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTT‑GCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCAcagcagcag                                                                                                                                                                                                                                      <  2:937488/238‑1 (MQ=255)        agCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTT‑GCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGCAGTTCATTAGCAACACGATACACCTCATCCGGCGTCATCGGGTGAGCCCCGTTACGGACTGCCCCCCccaccaaccgc                                                                                                                                                                                                                                     >  1:770377‑M1/1‑228 (MQ=255)        agCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTGGCTGCGGAGTCGGCTGAGCGATCCCCGTT‑GCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGGCCCATCTGACTAAGACGTTCAGTAGCACCACGAGGCACCTCTTCCTGCGTCATCGGGTTAGCGCCGTTCCGCCCTGCCCCCcacacccgccga                                                                                                                                                                                                                                     >  1:1010944/1‑231 (MQ=255)          cGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTT‑GCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTG‑CCCCCAcagcagcagata                                                                                                                                                                                                                                   >  2:875514/1‑235 (MQ=255)             ttggcagcaGGCTGTGTCGGCGCAGCAGGGCCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCCGAGTCGGCTGAGCGATCCCCGTT‑GCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGAt                                                                                                                                                                                                                                 <  2:175383/235‑1 (MQ=255)             ttAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTT‑GCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGAt                                                                                                                                                                                                                                 <  2:708518/238‑1 (MQ=255)                         tgtgTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTT‑GCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACACCAGCAGCAGATAACGCAAACGtt                                                                                                                                                                                                                     >  2:712641/1‑238 (MQ=255)                          gggtCGGCGCAGCAGGCGCAGGAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTT‑GCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTg                                                                                                                                                                                                                    <  2:922133/236‑1 (MQ=255)                                 cgcAGCAGGGCCAGTAGCTACATTTCCTGGCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTT‑GCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCt                                                                                                                                                                                                             <  2:822847/238‑1 (MQ=255)                                 cgcAGCAGGCGCAGTAGCTACATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTT‑GCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGCTGCACCTCTTCCGCCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCCCAGCAGCACCCGATAACGCAAAAGTTGAAGCCCtt                                                                                                                                                                                                            >  1:822847/1‑238 (MQ=255)                                          cgcAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTT‑GCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGa                                                                                                                                                                                                    <  2:831058/238‑1 (MQ=255)                                           gcgcgggCTGCATTTCCTGGCGCCGGTTGCTGCGGCGTCGGCTGAGCGATCCCCGTT‑GCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGAc                                                                                                                                                                                                   <  1:323119/232‑1 (MQ=255)                                           gcAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTT‑GCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGAc                                                                                                                                                                                                   <  2:285855/238‑1 (MQ=255)                                           gcAGTAGCTGCATTTCCTGGCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTT‑GCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGAc                                                                                                                                                                                                   <  2:736287/238‑1 (MQ=255)                                             aGTAGCTGCATTTCCCGTCGCCGGTTGCTGGGGAGTCGGCTGAGCGTTCCCCGTT‑GCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGAcgc                                                                                                                                                                                                 <  2:391287/238‑1 (MQ=255)                                              gTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTT‑GCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACgcgc                                                                                                                                                                                               <  2:144884/239‑1 (MQ=255)                                                    tcATTTCCTTGCTCCGGTTGCCGCGGTGGCCGTGGAGGAGGCCCCGTT‑GCCTGTTTAGGCAACCTTGGCACCAACGCGTCCGGAGAAGGCAATGCCTGCGGCATCACCTTCATCAGCCGTTCATTAGACACGCGATGCCCCTCTGCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTGGCAGCCCTGCAGTCGGACGCGCCTGAAt                                                                                                                                                                                         <  1:952406/238‑1 (MQ=255)                                                    gCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTT‑GCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAAt                                                                                                                                                                                         <  2:1015764/239‑1 (MQ=255)                                                      tttttccgTCCCCGTTTGTGGCGGGGTCGGGTGGGCGATCCCCGTT‑GCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATca                                                                                                                                                                                       <  1:516983/232‑1 (MQ=255)                                                      aTTTCCTGTCGCCGGTTGCTGCGGAGCCGGCGGAGCGATCCGCGTT‑GCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATca                                                                                                                                                                                       <  2:948544/239‑1 (MQ=255)                                                       tttCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTT‑GCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCGGCCGGCCCGCCCTGAACcac                                                                                                                                                                                      >  2:1005393/1‑239 (MQ=255)                                                       tttCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTT‑GCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAAACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTTCCCCCCACAGCACCAGCAGGTAACGCAAACGTTGCAGCCCTGCAGCCGGACCGGCCTGAACcac                                                                                                                                                                                      >  1:1015764/1‑239 (MQ=255)                                                                                tCGGCTGAGCGATCCCCGTT‑GCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACCGTATAAACGCGCTGGGGATCGAGt                                                                                                                                                             >  2:726562/1‑239 (MQ=255)                                                                                    gctgcgcctCCCCGCTGGCCGGTTCGGGGAACGTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCCTCTGCATCAGACTGTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGtttt                                                                                                                                                          <  1:712641/231‑1 (MQ=255)                                                                                                             aGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATTCCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGAt                                                                                                                                 <  2:896911/239‑1 (MQ=255)                                                                                                                        tccccATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGCTGCAGCCCGGCAGTCGGACGCGCCAGCATCACAGTAAAAACGCACTGGGTACCGCTGTTTACGGAAAAGCAAACCCCACCCGGACAACCTCCCggg                                                                                                                      >  1:824649/2‑239 (MQ=255)                                                                                                                        gCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTAAGGAAAAGAAAACCCCGACCGGAAAGCCTTCCggg                                                                                                                      >  1:394366/1‑239 (MQ=255)                                                                                                                             aTCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGAAAACCGCGACCGGATAGCCTTCCGGGAAAc                                                                                                                  >  2:611422/1‑238 (MQ=255)                                                                                                                                            ggCCATCCCCGCGGCATCATCCGCATCAGCCGTTCATTAGCAACCCGGTGCCCCTCTTCCGGCCGCATCGGGTTAGCGCCGTTACGAACTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCgg                                                                                                   <  1:785931/238‑1 (MQ=255)                                                                                                                                               aaTACCGGCGGCATCATCTGCACCAGACGTTCATTAGCAACACGATGCCCTCCTGCCGGCGTCATCGGGTTAGCGCCGTTACGATCTG‑CCCCCacagcagcagataacgcaaacgttgcagccctgcagtcggacgcgcctgaatcacagtataagcgcgctgggtatcgagttttacggaagagacaaccgcgaccggatagccttccgggaaacgaccgcccagaccggaagtcacc                                                                                              <  1:875514‑M1/239‑147 (MQ=255)                                                                                                                                                        ggCATCATCTGCATCAGCCGTTCATTAGCAACCCGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGcac                                                                                      <  2:518002/239‑1 (MQ=255)                                                                                                                                                        ggCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCCACTGAATCACATTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCCCAACAGGCAGGCCTTCCGGGACGCGCCCGCCCAGAcagaaaacccacaccac                                                                                      >  1:234676/1‑223 (MQ=255)                                                                                                                                                        ggCACCATCTGCATCAGACCTTTATTAGCAACACGATGCACCTCTTTCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTAGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGcac                                                                                      <  2:770377/239‑1 (MQ=255)                                                                                                                                                           atcatcTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGa                                                                                                                                       <  1:324254/187‑1 (MQ=255)                                                                                                                                                           atcatcTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGa                                                                                                                                       >  2:324254/1‑187 (MQ=255)                                                                                                                                                                tagcagccgccGTACATTAGCAACCCGATGCACCTCGTCCGGCGTCAGCGGGTTAGCGGCGTTACGATCTGCCCCCCACCGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGGCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCCCATCACCat                                                                              <  1:350804/229‑2 (MQ=255)                                                                                                                                                                cTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCgcagcagcagataacgcaaacgttgcagccctgcagtcggacgcgcctgaatcacagtataagcgcgctgggtatcgagttttacggaagagacaaccgcgaccggatagccttccgggaaacgaccgcccagaccggaagtcaccagca                                                                                          <  2:390151‑M1/227‑151 (MQ=255)                                                                                                                                                                         aCGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCAAAGTATAAGCGCGCTGGGGATCGAGTTTTACGGCAGAGACAACCGCGACCGGCAAGCCTTCCGGCAAACGACCGCCCAAACCAGAGGCCACCAGCAAACCACCAaccagcaac                                                                      >  1:74939/1‑231 (MQ=255)                                                                                                                                                                          cGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCgcaacagcagataacgcaaacgttgcagccctgcagtcggacgcgccggaatcacagtataagcgcgctgggtatcgagttttacggaagagacaaccgcgaccggcaagccttccgggaaacaaccgcccaaaccggaaggcaccagacaaccaccaacacagaccactaa                                                                    >  1:390151‑M1/1‑67 (MQ=255)                                                                                                                                                                           gTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGGCACAAGCACAACACCAACACGAataacg                                                                   >  2:885437/1‑236 (MQ=255)                                                                                                                                                                             tCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACcgc                                                                                                                                         >  2:344097/1‑167 (MQ=255)                                                                                                                                                                             tCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACcgc                                                                                                                                         <  1:344097/167‑1 (MQ=255)                                                                                                                                                                             tCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAACCGACCGCCCAGACCGGAAGTCACAAGCACATCACCAACACGAAAATCCga                                                                 >  2:398724/1‑238 (MQ=255)                                                                                                                                                                                       cacGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAACCGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGCAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCACAACGAAAATCCCTATTCGCCGGc                                                       >  2:667158/1‑239 (MQ=255)                                                                                                                                                                                       cacGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGCATCGAGTTTTACGGAAGAGACAACCGCGACCGGAAAGCCTTCCGGGAAACGACCCCCCAGACCGGAAGCCACAAGCAAATCACCAACACGAAAATCCGTATGCGCCgcc                                                       >  2:770087/1‑237 (MQ=255)                                                                                                                                                                                               ccccctTCCCGCTGCACCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTc                                               <  1:1005393/235‑1 (MQ=255)                                                                                                                                                                                                cccctTCCGGGGTTATCGGGTTAGCGCCGTCACGAACTGCCCCCCACAGCCGCAGCAGATAACGCAAACTTTGCAGCCCTGCATTCGGACGCGCCTGAATCACAGTATAAGGCCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACACCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCa                                              <  1:885437/236‑1 (MQ=255)                                                                                                                                                                                                 ctctTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCCCCAGCACATCACCAACACGAATATCCGTATTCCCCGGCAGCAGCTCa                                              >  2:384064/1‑238 (MQ=255)                                                                                                                                                                                                 ctctTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCCGAGATCACCAGCACATAACCAACACGAATATCCGTATTTGCCGCCAGGTGCTCa                                              >  2:923586/1‑238 (MQ=255)                                                                                                                                                                                                 ctctTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGAAAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGGCCCCAGCACATCACCAACACGAAAACCCCGATTCCCCgcgcgcagcccaa                                             >  1:7859/1‑227 (MQ=255)                                                                                                                                                                                                     tcccggcgcacccggTTAGCCCCGTTCCGATCTCCCCCCCCCAGCAGCAGAAGATAACGCAAACGTTGCCTCCATGCAGTCGGACCCGCCTGAAACACAATATAAGCGCGCTGGGTAGCGAGTTTTACGGAAGAGACAACCGCGACCTGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTg                                         <  1:667158/226‑1 (MQ=255)                                                                                                                                                                                                      ccGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGccctggctcttattcacat                                         >  2:837060/1‑221 (MQ=255)                                                                                                                                                                                                        ggCGTCATCGGGTTAAGCCCGTTACGATCCGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGGCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTAGCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCaa                                      <  2:761643/239‑1 (MQ=255)                                                                                                                                                                                                        gatggtttaaaggaacctcctttACGACCTGCCCCCCCACGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGGCGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCaa                                      <  1:232235/218‑1 (MQ=255)                                                                                                                                                                                                           gTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCCAatc                                   >  2:13596/1‑239 (MQ=255)                                                                                                                                                                                                                           cGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACCCAAACGTTGCAGCCCTGCAGTCGGACGGGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAATTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTa                   <  2:918546/239‑1 (MQ=255)                                                                                                                                                                                                                             ttACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGc                                           <  2:198941/213‑1 (MQ=255)                                                                                                                                                                                                                              tACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTAcc                 <  2:166010/238‑1 (MQ=255)                                                                                                                                                                                                                                     tGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAgcgcg                                                                                                                                                                          <  1:502187/78‑1 (MQ=255)                                                                                                                                                                                                                                     tGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAgcgcg                                                                                                                                                                          >  2:502187/1‑78 (MQ=255)                                                                                                                                                                                                                                     tGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAatcat                                 <  2:642682/215‑1 (MQ=255)                                                                                                                                                                                                                                     tGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAatcat                                 >  1:642682/1‑215 (MQ=255)                                                                                                                                                                                                                                     tGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTAcctttcccttatacccat          >  2:232235/1‑221 (MQ=255)                                                                                                                                                                                                                                       ccccccACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATACCCCGATTCGCCGGCAGATGTCCAACCGGCAAAACAcccagtctcttatacacaacgtcagc        >  1:198941/1‑215 (MQ=255)                                                                                                                                                                                                                                         ccccACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTAc     <  2:176409/239‑1 (MQ=255)                                                                                                                                                                                                                                            cacaGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGACAATCCGCATTCGCCGCCAGATGCTCAAACGGCCAAACATCCGAACAACCGTTACCTGCCCAAATTAcgcg  >  1:695881/1‑239 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                               TGTGGAGAGCGATTAGCAGCAGGCTGTGTCGGCGCAGCAGGCGCAGTAGCTGCATTTCCTGTCGCCGGTTGCTGCGGAGTCGGCTGAGCGATCCCCGTT‑GCCGGTTCAGGTAACTTTGGCCCCATCGCGTCTGGCGAAGGCAATACCTGCGGCATCATCTGCATCAGACGTTCATTAGCAACACGATGCACCTCTTCCGGCGTCATCGGGTTAGCGCCGTTACGATCTGCCCCCCACAGCAGCAGCAGATAACGCAAACGTTGCAGCCCTGCAGTCGGACGCGCCTGAATCACAGTATAAGCGCGCTGGGTATCGAGTTTTACGGAAGAGACAACCGCGACCGGATAGCCTTCCGGGAAACGACCGCCCAGACCGGAAGTCACCAGCACATCACCAACACGAATATCCGTATTCGCCGGCAGATGCTCAAGCTGCAAATCATCCGTACAACCGTTACCGGCTGCAATTACGCG  >  NC_000913/3398934‑3399406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.