Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 120,299 A→C 19.0% V418G (GTG→GGG)  aroP ← aromatic amino acid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913120,2990AC19.0% 155.7 / 2.6 69V418G (GTG→GGG) aroParomatic amino acid transporter
Reads supporting (aligned to +/- strand):  ref base A (45/8);  new base C (2/14);  total (47/22)
Fisher's exact test for biased strand distribution p-value = 1.85e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGCAGATACCACCAGCGCCATTAACAGTCCGAAAGCGGACTCTGGGGCAAGGTAGTTAATCAGTACGCACAACGCCGTTACCAGTGCAGACAC  >  NC_000913/120077‑120537
                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                               
ccTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAACCGAGACCTAACACGATCAGCCATACCGGGATCAGGGATACCGAAATCGCCCTTCCTGGGGTCATCAGCATAATACACAGAACCGCCGCCaa                                                                                                                                                                                                                                 >  1:757541/1‑238 (MQ=255)
                      gATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAACCGACAACTAACACGATCAGCCATACCGTGATCAGGTATACCGAAATAGCCATTACCGGGGGCATCAGCATAATACACAGTACCCCCCCCCAAAAcaaccagcgaagccagtta                                                                                                                                                                                                           >  1:325121/1‑222 (MQ=255)
                         tGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCCGGTATACCGAAATCGCCATTCCTTGGGTCCTCAGCATAATCACCAGTTCCGCCGCCATAAATAGCAAGCAGATCCAGGTAcca                                                                                                                                                                                                        >  2:303724/1‑238 (MQ=255)
                          gctctactGACGATTTGCGGGGCGTTGGTGTAAAGATTATTGCCCTCCCCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCTCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATCCCGGGATCAGGTATACCGAAATCGCCATCCCTGGGGTAATCAGCATAATCCCCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCa                                                                                                                                                                                                       <  2:251402/236‑1 (MQ=255)
                          gCACTAGTGGCGTTTGACGGGGCGTGGGTGTAAAGATTATTGCCCTCACCGTGTACGGGTGAGGGCGTAGAGATATTAAGGCGCTTTTCCGGTTTTGGCGGTTTTCTTTTTAAACAGATAGCCGATCCCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAAGCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCa                                                                                                                                                                                                       <  2:645278/239‑1 (MQ=255)
                          ccACTACTGAGGTTTTGCGGGGCTTTGGTGTAAAGTTTATTGCCCTCACCTTGTAGGGGTGAGGGCGTAGAGAGATTAATGCGTTTTTACGGCTTGGGCGGTTTTCTCTTTAAACAGATAGCCGATCCCTAACACGATCAGCCATCCCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCa                                                                                                                                                                                                       <  2:757541/238‑1 (MQ=255)
                            actactGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGTCGATACCTAACACGATCAGCCATACCGGGATCAGGGATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGAAGATCCAGTTACCCAGc                                                                                                                                                                                                     >  2:30986/1‑239 (MQ=255)
                                               ttaaaagaaacaggATTATTGCCCTTCCCTGTTACGGGTGAGGGCGTAGAGAAATTAAGGCGTTTTTACGGTTTGGGCGGTTTTCCTTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATCCCGAAATCGCCATTCCTGGGGTCATCAGCATAATCCCCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGa                                                                                                                                                                                 <  2:651495/226‑1 (MQ=255)
                                                 gTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCCTAAACAGCAGGCAGATCCAGTTACCCACCGGATAAAGCAGAGCAGGGaa                                                                                                                                                                                >  2:206606/1‑238 (MQ=255)
                                                                  tGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTAccg                                                                                                                                                                                                                                        <  2:27244/166‑1 (MQ=255)
                                                                       tCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCCTAATCACCCGAACCGCCGCCCTAAACAACCGGCAGATCCAGTTAACCAGCCGATAAAACAGAGCAGGGAAAGGAGGTACCCCggcccttaaac                                                                                                                                                         >  1:651495/1‑229 (MQ=255)
                                                                         aCCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCAATTACCCAGCGGATAAAGCAAGGAAGGGAAGGGAGTTACACCGCCTTGGTCCt                                                                                                                                                         >  2:757456/1‑237 (MQ=255)
                                                                              gTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATAACGAAATCGCCATTCCCGGGGTCCTCAACATAATAACCAGTAACGCCGCCCTAAAAAGCAGGCAGAACACGTTTCCCCACGGGTAAAACACAACAGGGAAACGAGTTACACCGCCttttccacgacttgg                                                                                                                                                  >  1:492984/1‑226 (MQ=255)
                                                                              gTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAAAAGGTAGGCGATACCTAACACGATCAGCCATACCGGGATCAGGGATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCCTAAACAGCAGCCAGATCCAGTTACCCAGCGGCTAAAGCAGAAACAGGAAGAGAGTTACCACGCCATGTTCCCGCCTGgc                                                                                                                                                  >  1:100436/1‑239 (MQ=255)
                                                                                tACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCCGATCCAGGTACCCCGCGGATAAAGCAAAGCAGGGAAGCGAGTTACCACGCCCTggttccgactggg                                                                                                                                                  >  2:242908/1‑226 (MQ=255)
                                                                                tACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAACCGATACCTAAAACGATCAGCCATACCGGGATCAGGGATACCGAAAACGCCATTCCTGGGGTCATACGCCTAATCACCAGTACCCCCCCCCCAAACAGCAGGCAGAGCCAGTTACCCAGCGGAAAAAGCAAGGCGGGGAAACGAGGTACCACGCCTTTTTCCCGCTTgggg                                                                                                                                                 >  1:932764/1‑237 (MQ=255)
                                                                                     gtggggggttAGAGATATTAATGCTTTTTTCCGGCTTTGGGGTTTTTCCTTTAAAACAGATGGCCGATACTTAACACGATCAGCCATACCGGGATCAGTTATCCCGAAATCGCCATCCCGGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACg                                                                                                                                                                   <  2:575705/207‑1 (MQ=255)
                                                                                          ggCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTTTCTTTAAACAAATAGCCGATACCTAACACGATCAGCCATAACGGGATCAGGTATACCGAAATCGCCATTCCTGGGGGCATCAGCCTAATCACCAGTACCCCCGCCATAAACAGCAGGCCGATCCAGTTACCCCCCGGATACACCCAAGCAGGGAAACGCAGTACCAACCCTTTTTCCCGCTTGGggcgcagagaat                                                                                                                                       >  1:286062/1‑231 (MQ=255)
                                                                                          ggCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAag                                                                                                                                                                                             >  2:201220/1‑186 (MQ=255)
                                                                                                 ggggtattaTGCCCTTTTTCGTGTTTGGGCGTTTTCCTTTTAAAACAGATCGCGGACCCCAAACACGACCACACATCCGTGGAACAGGTATCCCGAATCCGCCATTCGTGGGGTCATCAGCATAATCCCCAGTCCCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCata                                                                                                                                  <  1:826819/230‑1 (MQ=255)
                                                                                                   aaaTTAATGGTTTTTTCCGGTTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATCCCGACCACGACCAGCCAACCGGGGAACAGGTAGCCCAAAATCGCCTTTCCGGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATg                                                                                                                                <  2:492984/235‑1 (MQ=255)
                                                                                                     aTTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGGCATCAGCATAATCAACAGTACCGCCGCCATAAACAGAAAGCAGATCCATGTACCCAGCGGGAAAAGCAGAGCCGGGGAACGAGATACCACGCCTTGTTCCTGCTTGGCGCGCCGGAATTTCATATgaccc                                                                                                                            >  1:555294/1‑235 (MQ=255)
                                                                                                          tGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCCTATGCGCCCGGCt                                                                                                                       >  2:127285/1‑239 (MQ=255)
                                                                                                             gcTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCGGTTACCCAGCGGATAAAGCAGAGCAGGGAAGAGAGTTACCACGACTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAat                                                                                                                    >  2:124866/1‑239 (MQ=255)
                                                                                                                     ggCTTTGGCGTTTTTCTTTTAAAACAGATAGCCGATACTTAACCCGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCTTTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGccc                                                                                                            <  2:100436/239‑1 (MQ=255)
                                                                                                                      tataaagtctcttttCTTTTTAAACAGATCCCCTATCCCAAACGCGATCCGCCACCCCGGGAACGGGTATCCCGAACTCCCCATCCCGGGGATCAACAGCATAACCCCAAGTCCCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGccc                                                                                                            <  1:436591/228‑1 (MQ=255)
                                                                                                                      ggttttGGCGGTTTTCTCTTAAAACAGATAGCCGATCCCTAAACCGATCAGCCATCCCGGGATAGGGTAGACCGAACTCGCAATTCCGGGGGTCATCAGCATAATCCCCAGTCCCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGccc                                                                                                            <  2:286062/236‑1 (MQ=255)
                                                                                                                          ttGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGCAGTACCACGCCCTGTTCCTGCTTTGCCCGACGGAATTTCCAATGCGCCAGGCTAATCATCACCCCGtt                                                                                                        >  2:501665/1‑239 (MQ=255)
                                                                                                                          ttGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTACTGGGGTCATCAGCATAATCACCCGTACCGCCGCCATAAACAGCAGGCAGATCCAGCTAACCAACGGGTAAAGCAGCACAGGCAAAGCGGTTACCACACCTTGTTCCTGCTTATGGCGACCGAATTTCAAAGGGGCCCGCCCAAACACCCCCCAGtt                                                                                                        >  1:182079/1‑239 (MQ=255)
                                                                                                                            ggCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAag                                                                                                                                                                                             >  2:502604/1‑153 (MQ=255)
                                                                                                                                gTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATaaa                                                                                                                                                                                                                              >  2:427632/1‑116 (MQ=255)
                                                                                                                                 ttttttttttAAACAGATTGCCTAACCCAAAACCGACCCCCCAACCCGTGATCGGGTAAACCAAACCCGCCTTTCGGGGGGTCATCAGAATAACCACAAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTAcc                                                                                                 <  1:30986/239‑1 (MQ=255)
                                                                                                                                             aCAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTAc                                                                                                  >  2:436591/1‑226 (MQ=255)
                                                                                                                                                         tACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGTTTACCCGGGCAGATACCACCACCGCCAtt                                                                         >  2:932555/1‑239 (MQ=255)
                                                                                                                                                               acacGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCCTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGCAGATACCACCAGCGCCATTAACAg                                                                    >  2:469805/1‑238 (MQ=255)
                                                                                                                                                                     tCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGCAGATACCACCAGCGCCATTAACAGTCCGaaa                                                             >  2:537578/1‑239 (MQ=255)
                                                                                                                                                                     tCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCAGCGCCCAGTTGATTACCAGTGCAGATACCACCAGCGCCCTTACCAGTCCGcaa                                                             >  2:976805/1‑239 (MQ=255)
                                                                                                                                                                        gCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACCAGGGCAGATACCACCAGCGCCATTAACAGTCCGAAAGCg                                                          >  2:51677/1‑239 (MQ=255)
                                                                                                                                                                         cattACCGGAACCAGGTGACCCGAAACCGCCATCCCGGGGTTAACAGAAATAATCCCCAGTCCCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGCAGATACCACCAGCGCCATTAACAGTCCGAAAGCg                                                          <  2:113857/235‑1 (MQ=255)
                                                                                                                                                                             aCCGGGCTCAGGTATACCGAAATCGCAATTCCTGGGGTCATCCGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATACAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGAGCGACGGAATTTCATATGCGCCAGGACAATCACCGCCCCGTTGATTACCAGTGACATTACAACCAGCGCCATTACAAGGCCGAAAGAggcccc                                                     >  1:724519/1‑235 (MQ=255)
                                                                                                                                                                             aCCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGCAGATACCACCAGCGCCATTAACAGTCCGAAAGCGGActc                                                     >  2:614817/1‑239 (MQ=255)
                                                                                                                                                                             aCCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACCAGTCCAGTTACCACCCGCGCCATTAACAGTCCGAAAACGGAccc                                                     >  1:861533/1‑237 (MQ=255)
                                                                                                                                                                                gggATCAGGTATCCCGAAACCCCCATTCCGGGGGTCATCAGCATAACCCCCAGTCCCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAag                                                                                                                                                                                             <  1:358138/101‑1 (MQ=255)
                                                                                                                                                                                   aTCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCTGATCCAGTTACCCAGCGGATAAAGCAGCGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATCTGCCAGCCTAATCATCGCCCCGTTGATTCCCAGTGCCATTACAACCAGCGCCCTTAACacaccaaaacagatcccctggg                                                >  1:113857/1‑217 (MQ=255)
                                                                                                                                                                                   aTCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACAAGGGCAGATACCACCAGCGCCATTAACAGTCCGAAAGCGGACCCTGGGGc                                               >  2:756620/1‑239 (MQ=255)
                                                                                                                                                                                   aTCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCCAGTTACCAAGCCTTGTTCCTGCTTGGCGCCACGGAATTTCCTATGAGCCAGGCTAATCATCGCCCAGTTGCTTACCAATGCCGCAACCACCCGCCCCCATAACAATACGAAAACGGCCACTGGGGc                                               >  1:70826/1‑239 (MQ=255)
                                                                                                                                                                                   aTCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAAGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTAACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGGGCCAGGCTAATAATCGCCAAGTTGATTACCAGGGCAGTAACACCCATGCCCATTAACAGACCGAAAGGGAGCCCTGGGGc                                               >  1:771901/1‑239 (MQ=255)
                                                                                                                                                                                     cAGGTATCCCAAACCCGCCATTCTGGGGGCAATCAGAATAACCCCCATCACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGCAGATACCACCAGCGCCATTAACAGTCCGAAAGCGGACTCTGGGGCaa                                             <  2:555294/239‑1 (MQ=255)
                                                                                                                                                                                      aGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCCGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGCAGATACCACCAACGCCATTAACAGTCCGAAAGCGGACCCTGTGGCAAg                                            >  2:353119/1‑239 (MQ=255)
                                                                                                                                                                                      aGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGAGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGCAGATACCACCAACGCCCTTAACAACCCGAAAAGGGACCCTGGGGaaaa                                            >  1:309909/1‑238 (MQ=255)
                                                                                                                                                                                        gtaccccGAAATCGCCATCCCTGGGGCCCTCAGCAAAATCCCCAGTCCCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGa                                                                                                                                              <  1:288974/134‑1 (MQ=255)
                                                                                                                                                                                         tataCCGAAATCGCCATCCCGGGGGTCATCAGCAAAACCCCCAGTCCCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGCAGATACCACCAGCGCCATTAACAGTCCGAAAGCGGACTCTGGGGCAAgg                                           <  2:325121/237‑1 (MQ=255)
                                                                                                                                                                                          ataCCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAggg                                                                                                                                                                                  >  2:358138/1‑101 (MQ=255)
                                                                                                                                                                                           taCCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGCAGATACCACCAGCGCCATTAACAGTCCGAAAGCGGACTCTGGGGCAAGGTAGt                                       >  2:365389/1‑239 (MQ=255)
                                                                                                                                                                                            aCCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTAcc                                                                                                                                                                                                         >  2:822257/1‑77 (MQ=255)
                                                                                                                                                                                              caaaacccccATTCCGGGGTTCACAACCAAAACCCCAAGTCCCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGCAGATACCACCAGCGCCATTAACAGTCCGAAAGCGGACTCTGGGGCAAGGTAGTTaa                                    <  2:932764/231‑1 (MQ=255)
                                                                                                                                                                                                aaaTCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAata                                                                                                                   >  2:5278/1‑157 (MQ=255)
                                                                                                                                                                                                aaaTCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGCAGAt                                                                                        >  2:762274/1‑185 (MQ=255)
                                                                                                                                                                                                aaaTCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGCAGATACCACCAGCGCCATTAACAGTCCGAAAGCGGACTCTGGGGCAAGGAAGGTaacc                                  >  1:149425/1‑237 (MQ=255)
                                                                                                                                                                                                aaaTCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGCAGATACAACCAGCGCCATGAACAGTCCGAAAGCGGACTCTGGGGCAAGGGAGTTAATc                                  >  2:845504/1‑239 (MQ=255)
                                                                                                                                                                                                  aTCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGa                                                                                                                                                                                                  >  2:966259/1‑78 (MQ=255)
                                                                                                                                                                                                  aTCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCa                                                                                                                                    >  2:288974/1‑139 (MQ=255)
                                                                                                                                                                                                     gCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTAc                                                                                                  >  2:997217/1‑170 (MQ=255)
                                                                                                                                                                                                          tcgggggcactcgaGCATAACCCCCAGTCCCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGCTGATACCGCCAGCGCCATTAACAGTCCGAAAGCGGACTCTGGGGCAAGGTAGTTAATCAGTACGcaca                        <  2:182079/226‑1 (MQ=255)
                                                                                                                                                                                                          tCCTGGGGTCATCAGCATAACCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGCAGATACCACCAGCGCCATTAACAGTCCGAAAGCGGACTCTGGGGCAAGGTAGTTAATCAGTACGcaca                        <  2:861533/239‑1 (MQ=255)
                                                                                                                                                                                                          gccgggggACATCAACAAAACCCCCAGTCCCGCCGCCATAAACAGCAAGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGCAGATACCACCAGCGCCATTAACAGTCCGAAAGCGGACTCTGGGGCAAGGTAGTTAATCAGTACGcaca                        <  2:771901/235‑1 (MQ=255)
                                                                                                                                                                                                          cccTGGGGACCACAGCAAATCCCCCGGTACCGCCGCCATAAACAGCAGGCAAGTCCAGTTACCCAGCGTATAAAGCAGAGCAGGGAAGCGAGTGACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGCAGATACCACCAGCGCCATTAACAGTCCGAAAGCGGACTCTGGGGCAAGGTAGTTAATCAGTACGcaca                        <  2:452704/238‑1 (MQ=255)
                                                                                                                                                                                                                    atcaGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGCAGCGGGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGTAGATACACCCCGCGCCATTATCAGTCCGAAACCGACCACTGGCGAAAGGCCGTTAAACATTAAGccaaaccacgtaac              >  1:383301/1‑226 (MQ=255)
                                                                                                                                                                                                                    atcaGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGCAGATACCACCAGCGCCATTAACAGTCCGAAACCGGACCCTgggg                                                >  2:645106/1‑205 (MQ=255)
                                                                                                                                                                                                                                 aCCAGACCCGCGCCCATAAACAGCGGGCAGATCCAGTTGCCCAGCGGATAAAGAAGAGCAGGGAAGGGATTTGCCCCGCCTTGTTCCTGGTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTCCCAGTGCAGATACCACCAGCGCCATTAACAGTCCGAAAGCGTACTCTGGGGCAAGTTAGTTAATCAGTACGCACAACGCCGTTACCAGTGCAGacac  <  1:303724/239‑1 (MQ=255)
                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                               
CCTCGTTCGTGGTCGTGGTGGTGATTGCACTACTGACGATTTACGGGGCGTTGGTGTAAAGATTATTGCCCTCACCCTGTACGGGTGAGGGCGTAGAGAGATTAATGCGCTTTTACGGCTTTGGCGGTTTTCTCTTTAAACAGATAGCCGATACCTAACACGATCAGCCATACCGGGATCAGGTATACCGAAATCGCCATTCCTGGGGTCATCAGCATAATCACCAGTACCGCCGCCATAAACAGCAGGCAGATCCAGTTACCCAGCGGATAAAGCAGAGCAGGGAAGCGAGTTACCACGCCTTGTTCCTGCTTGGCGCGACGGAATTTCATATGCGCCAGGCTAATCATCGCCCAGTTGATTACCAGTGCAGATACCACCAGCGCCATTAACAGTCCGAAAGCGGACTCTGGGGCAAGGTAGTTAATCAGTACGCACAACGCCGTTACCAGTGCAGACAC  >  NC_000913/120077‑120537

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: