Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,085,014 T→C 14.5% E171G (GAG→GGG)  yeeE ← UPF0394 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,085,0140TC14.5% 163.2 / 3.4 73E171G (GAG→GGG) yeeEUPF0394 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (20/40);  new base C (7/5);  total (27/46)
Fisher's exact test for biased strand distribution p-value = 1.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTG  >  NC_000913/2084780‑2085241
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                   
ttAATTTTTTTGATGGCACCCGCGACCAGAAATTGCAGAATATTTGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCttt                                                                                                                                                                                                                                 <  1:15825/239‑1 (MQ=255)
tgaaTGTAGTTCAGGTCCCCCGCGACCAGAAATTGCAGGATATTGGCCGTTGGGAAAGGGATTCCGAACCCAAACATGCGTCCGGTTGCTTCCCTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCttt                                                                                                                                                                                                                                 <  1:77872/237‑1 (MQ=255)
 tAATGTATTTCATGTCACCCGGGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCttt                                                                                                                                                                                                                                 <  2:237594/238‑1 (MQ=255)
 tAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGTAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCttt                                                                                                                                                                                                                                 <  2:867803/238‑1 (MQ=255)
       aattCATGTCCCCCGCGACAAGAAATTGCAAAATATTGGCCGTGGGGGAAGTTATTCCTAACCCAAACAGGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATcac                                                                                                                                                                                                                           <  2:495924/236‑1 (MQ=255)
         ttCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCCGGGGCCCGGCTAAAAGCCCAATCAAACCGCTGAGTCCACCTGTGACAAAGGGATGCAACCGTTTTTCAAACAGAATATAAGGGATCCCGGTTCGTGCCGGCGGTAAGGTGACGCCTTGTAGTTTTGGCTTCTTCAACCTTTtctctcccc                                                                                                                                                                                                                         >  1:495924/1‑231 (MQ=255)
         ttCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGATAACAGCCGTGACAAAGGGATGCCAGCGGTTTTCAAAAAGAATATGATCGATCCCGGGTCGGCGCGGCGGTAAGGTCGCGCCTTTGAGCTTTTGCTTCTTCAACCATTTTATcaccc                                                                                                                                                                                                                         >  2:625362/1‑237 (MQ=255)
         ttCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGTTGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGTGGGCGGGGAAGGTCGCGACTTTGAGTTTTGGCCTTTTCAACTCTTtcatccacc                                                                                                                                                                                                                         >  1:747434/1‑234 (MQ=255)
         ttCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACCCTTTCATcacca                                                                                                                                                                                                                         >  2:392926/1‑238 (MQ=255)
         ttCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGAGATCCCGGTTCGGCGCGGCGGTAAGGTCGAGACTTTGAGGTTTGGCTTCTTCAAct                                                                                                                                                                                                                                     >  2:349108/1‑226 (MQ=255)
                cACTCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGCGATTCCTAACCCAAACAGGCGTCCGGATGCTTCACACAGTGGCCAGGCTCAAAGCGCAATCAAACCGCTGAGACCAGCTGTGCAAAAGGGATGCCAGCGCTTTTCCAACAGAATCTGAGAGATCCCGGTCCGGCCCGGCGGTAAGGTGGAGACTTTGAGTTTTGCCTTGTTCAACTttttttgcaacaccacagac                                                                                                                                                                                                                 >  1:670906/1‑223 (MQ=255)
                cACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGCCAAAGGGATGACAGCGTTTTTCAAACCGAATATGAGCGATCCCGGTTCGGCGCGGCGGAAAGGTCGCGACTTTGAGTTTTTGCTTCTTCAACCCTTTCCTCAACACCCaacaa                                                                                                                                                                                                                 >  1:7374/1‑235 (MQ=255)
                cACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTTAACCCTTTCATCACCCCCCaagac                                                                                                                                                                                                                 >  2:470240/1‑235 (MQ=255)
                cACCCCCCACCCGAAATTTCCTAATTTTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTTCTCCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCag                                                                                                                                                                                                                    <  1:866760/236‑1 (MQ=255)
                  cccGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTGGGCGCGCGGGGAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACCCTTTcaccacacaccaaagggt                                                                                                                                                                                                               >  1:338418/1‑227 (MQ=255)
                      cgACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATc                                                                                                                                                                                                           <  2:338418/239‑1 (MQ=255)
                                                  tGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGcaaccaaagtcccc                                                                                                                                                                                >  2:894638/1‑231 (MQ=255)
                                                    gggAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATc                                                                                                                                                                                                           <  2:588474/209‑1 (MQ=255)
                                                     gcaggTGATTCCGAACCCAAACCAGCGTCCGGTTGCTTCACTTAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCcacaca                                                                                                                                                                             <  2:380384/234‑1 (MQ=255)
                                                       aaGTGATTCCCAACCCAAAATGGCGTCCGGGTGGTTCCCTCGGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGACCGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGa                                                                                                                                                                           <  1:565314/238‑1 (MQ=255)
                                                         ttGATTCCTAACCCAAACAGGCGTCCGGTTGCTTCACTAAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGcc                                                                                                                                                                                  <  2:851190/228‑1 (MQ=255)
                                                              tCCTAACCCCAACAGGGGTCCGGTTGCTTCACCCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATCAGTACAGCTGTGCCAAAGGGATGCCAGCGTTTTTCAAACAGAATATGACCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTaa                                                                                                                                                                   <  1:34677/239‑1 (MQ=255)
                                                              tCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCCCACAGACAAATTaa                                                                                                                                                                   >  2:727475/1‑239 (MQ=255)
                                                                       aacaaaccGTCCGGTTGCTTCCCTCAGGGGCCAGGCTAAAAGCCAAATTAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAg                                                                                                                                                          <  1:252003/232‑1 (MQ=255)
                                                                        aaCATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCACCAAAAAGGCAACCAACGGCCCCACAGCAAAATTaaaaagttcagc                                                                                                                                                         >  2:615225/1‑231 (MQ=255)
                                                                        aaCATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGCCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGGTATCCCCAGCAAAACGGCAACCAACGGCCAAACAGAAAAATAaaaaaatcaaac                                                                                                                                                         >  1:716115/1‑231 (MQ=255)
                                                                          cactcctCCGGTTGCTTCACTCAGGGGCCAGGATAAAAGCCCAATCAAACCGATTAGTACAGCGGTGACAAAGGGATGCCAGCGTTTTGCAAACAGAATATGGGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTACAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCaa                                                                                                                                                       <  1:88880/233‑1 (MQ=255)
                                                                            tgccgccggttctttaccccAGGGGCCAGGCTAAAAGCGCAATAAAACCGAGGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATa                                                                                                                                                     <  1:470240/220‑1 (MQ=255)
                                                                               gtacgtttGCTTCCCCCAGGTGCCAGGTTAAAAGGCAAATCAAACCGATGAGTTCAGCGTTGACAAAGGGGTGCCAGCGTTTTGCAAACAGAAGATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATagag                                                                                                                                                  <  1:149167/233‑1 (MQ=255)
                                                                                 ctggTTGCATCACGTAGGGGCCAGGATAAAAGGGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTAAAAAAGAATATTGGCGATCCCGGTTCGTCGCGGCGGTAAGGTCGCGACTTTTAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAAGTAAAAGTCACAGCAATAGAGtt                                                                                                                                                <  1:1021369/237‑1 (MQ=255)
                                                                                 ccGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGtt                                                                                                                                                <  2:1008411/239‑1 (MQ=255)
                                                                                 ccGGTTGCGTCAAGTAGGGGCCATGCTAAAAGCGCAATCAAACCGAATATTACAGCTGTGACAAAGGGATGCCAGCGGTTTTCAAACAGAATATGAGGTATCCCGGTTCGGCGCGGCGGTAAGGGCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGtt                                                                                                                                                <  2:670906/239‑1 (MQ=255)
                                                                                 ccGGTTCCTTCCCTTAGGGGCCAGGCTAAAAGCGCAATCAAACCGAGGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGGGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGtt                                                                                                                                                <  1:615225/239‑1 (MQ=255)
                                                                                  cGGTTGCTCCACTCAGGGGCCAGGTTAAAAGCGCAATCAAACCGAAGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACTGAATAGGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGtt                                                                                                                                                <  2:451536/238‑1 (MQ=255)
                                                                                     ttGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTAttt                                                                                                                                            >  2:198968/1‑237 (MQ=255)
                                                                                      ttcTTCACTCGGGGGCCAGGTTAAAAGCGCAATTAAACCGAGGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCTGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGTGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGtt                                                                                                                                           <  1:392926/237‑1 (MQ=255)
                                                                                      tGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGGTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGtt                                                                                                                                           <  2:747434/239‑1 (MQ=255)
                                                                                        cTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCcaca                                                                                                                                                                               <  2:874007/201‑1 (MQ=255)
                                                                                         ttCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAg                                                                                                                                        <  1:198968/239‑1 (MQ=255)
                                                                                              tcgggggTCAGGCTAAAAGCGCAATCAAACCGATAGGTACAGCTTTGACAAAGGGATGCCGGCGTTTTTCAAACAGAATATGGGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTg                                                                                                                                   <  1:231885/236‑1 (MQ=255)
                                                                                                acgggCCAGGCTAAAAGCGCAATCAAACCGATGATGACAGGTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATAGGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGGCTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCTCCCAGATCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTg                                                                                                                                   <  2:397629/235‑1 (MQ=255)
                                                                                                       aGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGtgctg                                                                                                                           <  2:7374/238‑1 (MQ=255)
                                                                                                            aaaaGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGt                                                                                                                     <  2:131834/239‑1 (MQ=255)
                                                                                                                   cAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAAAGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTAAGTTACGTACTGTAGGGATGCAAGGTTTTAttt                                                                                                              >  2:985751/1‑239 (MQ=255)
                                                                                                                   cAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAAACCTTTCCTCCCCCCCCAGAACGTTATCACCCGCAAAACGGCAACCAACGGCCACACAGCCCACTTAAAAGGCTCCGCAATAAAGCAATGTCCAATCCCTTACACCTGCAAGGTTTGAttt                                                                                                              >  1:32671/1‑239 (MQ=255)
                                                                                                                   cAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGGAAGGGCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCCTCACCACCCAGAGCGTTATCACCCGCAAAACGGCAACCAACGACCACACAGACAAATTAAAAGTCTCGACAAAAAAGTTATGTTAAATACTATAGGGCCGCAAGGGTTTAttt                                                                                                              >  1:104245/1‑239 (MQ=255)
                                                                                                                         acactgtggatctgagttgtgaaaaGGGGAGGCAGCGTTTTTCAAACTGTATTGGAGCGATCCCGGTTCGCGGCGGCGGACAGGTCGCGACGTTGACTTTTGGATACTTCAACCCTTTCAGCACCACCCAGAGCGTTAACGCCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAATTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCa                                                                                                        <  1:625362/217‑1 (MQ=255)
                                                                                                                           ccGATGAGTACAGTTGTGACAAAGGGATGCCAGCGTTTATCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACt                                                                                                      <  2:104245/239‑1 (MQ=255)
                                                                                                                           ccGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGATTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACt                                                                                                      <  2:716115/239‑1 (MQ=255)
                                                                                                                              ttgtgttctgCTGTGCCAACGGGTTGCCAGGGTTTTTCAAACATAATATGGGCGAGCCCGGTTCGGCGCGGCGGTAAGTTCGCGCATTTGAGTTTTGGCTTCTCGAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGc                                                                                                   <  1:280907/230‑1 (MQ=255)
                                                                                                                                      aGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATcac                                                                                                                                                                                                                           >  1:439872/1‑111 (MQ=255)
                                                                                                                                      aGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATcac                                                                                                                                                                                                                           <  2:439872/111‑1 (MQ=255)
                                                                                                                                             aCAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGCCAAATTAAAAGGCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCa                                                                                     >  2:100501/1‑238 (MQ=255)
                                                                                                                                             aCAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACAACTGGCATGTGGGGAACGCAt                                                                                    >  2:99671/1‑239 (MQ=255)
                                                                                                                                                    cgtGCCAGGGTTTTTCAAACGGAATATGCGCGTGCCCGGTCCGGCGGCGCGGTAAGGTCCCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCAACACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCg                                                                               <  1:72142/235‑1 (MQ=255)
                                                                                                                                                                                    aTCCCGGTTCGGCGCGGCGGTAAGGTCGCGGCTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCATACAGACAAATTAAAAGTCTCAGCAATAGAGTTATg                                                                                                                                             <  1:629867/143‑1 (MQ=255)
                                                                                                                                                                                    aTCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACTGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATg                                                                                                                                             >  2:629867/1‑143 (MQ=255)
                                                                                                                                                                                    aTCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGCCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGGGAAAAGCGCGATCCa                                             >  2:57369/1‑239 (MQ=255)
                                                                                                                                                                                            tCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAACCCGATCCAACTGCCg                                      >  2:280275/1‑238 (MQ=255)
                                                                                                                                                                                              ggcgcggcgGTTAGGTCGCTACTTGTGGGTGTGGCCTCCTCCACCTTTTCCTCCCCCCCCAGAGCGTTACCACCAGCAAAACTGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGTATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATc                                   <  2:74056/239‑1 (MQ=255)
                                                                                                                                                                                              ggcgcggcgGTAAGGTCGCGCCTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATc                                   <  2:285519/239‑1 (MQ=255)
                                                                                                                                                                                              ggcgcggcgGTAAGGTCGCGACTTTGAGTTTTGGCCTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATc                                   <  2:32671/239‑1 (MQ=255)
                                                                                                                                                                                              ggcccggcgTAAAGGTCGCGACTTTGATTTTTTGCTTCTTCAACTCTTTCATCACCACCCAGAGCTTTATCCCCAGCAAAACGGCAACCAACGGCCACAAAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATc                                   <  1:727475/239‑1 (MQ=255)
                                                                                                                                                                                                cgcggctGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACGCTTTCATCACCAACCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGtt                                                                                                                                                <  1:378230/128‑1 (MQ=255)
                                                                                                                                                                                                cgcggcgGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGtt                                                                                                                                                >  2:378230/1‑128 (MQ=255)
                                                                                                                                                                                                cgcggcgGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACCCCTCACCATATAATGGAAAAGCCGGATCCAACTCCCGAtta                                  >  2:346287/1‑236 (MQ=255)
                                                                                                                                                                                                cgcggcgGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAATCCCAGCAATAGAGTTATGTTCAGTACCGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATTTGGAGAACGCATACCCCCCCCCATCAACATAAAAGTGAAAACCGGGAAcccaacgcccaaaaa                                 >  1:430008/1‑226 (MQ=255)
                                                                                                                                                                                                         aaGGTCGCGATTTTGAGTTTTGGTCTCTTAAACCCTTTCATCCCCCCCCAGAGGTGTAACCCCAGAAAAACGGCAACCAACGGCCCCACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGGGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCAcc                        <  1:894638/239‑1 (MQ=255)
                                                                                                                                                                                                             ccgcctcTTTGAGTTTTGGCTTTTTCAACTCTTTCATCACCACCCAGAGCGTTATCCCCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCcgcg                    <  1:57369/233‑1 (MQ=255)
                                                                                                                                                                                                              cgcgagttttgtttttGGCTTTTTCAACCCCTTCAACCCCACCCAGAGCGTTATCCACAGGAAAACGGCAACCAACGGGCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTGCAGTACTGTAGTGCCGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCcgcg                    <  1:42630/226‑1 (MQ=255)
                                                                                                                                                                                                                  aCTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCa                                                                                                                         >  2:375727/1‑133 (MQ=255)
                                                                                                                                                                                                                  aCTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCa                                                                                                                         <  1:375727/133‑1 (MQ=255)
                                                                                                                                                                                                                             tGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCCCACCCCCCCGAAACCAGGCCCCgg    >  1:548413/1‑239 (MQ=255)
                                                                                                                                                                                                                                cctcttcAACCCTTTCATCCCCACCCAGGGGGTTATAACCAACAAAACGGCAACCAACGGCCACACAGACAAAGTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGGAGTGCTGCAAGGTTTGATGTAAACCACGGGCAGGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTg  <  1:346287/236‑1 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                   
TTAATGTATTTCATGTCACCCGCGACCAGAAATTGCAGAATATTGGCCGTTGGGGAAGTGATTCCTAACCCAAACATGCGTCCGGTTGCTTCACTCAGGGGCCAGGCTAAAAGCGCAATCAAACCGATGAGTACAGCTGTGACAAAGGGATGCCAGCGTTTTTCAAACAGAATATGAGCGATCCCGGTTCGGCGCGGCGGTAAGGTCGCGACTTTGAGTTTTGGCTTCTTCAACTCTTTCATCACCACCCAGAGCGTTATCACCAGCAAAACGGCAACCAACGGCCACACAGACAAATTAAAAGTCTCAGCAATAGAGTTATGTTCAGTACTGTAGTGCTGCAAGGTTTGATTTAAACCACTGGCATGTGGAGAACGCATCACCGCACTCATCACCATATAAGTGAAAAGCGCGATCCAACTGCCGATCAATCCCTCACCCGCGCGATACCAGGTCCCGGTG  >  NC_000913/2084780‑2085241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: