Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,511,394 T→C 19.6% intergenic (+69/+72) ypeC → / ← mntH DUF2502 family putative periplasmic protein/manganese/divalent cation transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,511,3940TC19.6% 131.1 / 5.4 71intergenic (+69/+72)ypeC/mntHDUF2502 family putative periplasmic protein/manganese/divalent cation transporter
Reads supporting (aligned to +/- strand):  ref base T (36/18);  new base C (12/4);  total (49/22)
Fisher's exact test for biased strand distribution p-value = 7.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATCGCAATTATGAGTGGCGCAAAAACCGTTGGTGGCGTCATGATAATGGCTACCACCGTGGCTGGGATAAGCGTAAAGCGTATGAGCGTGGCTATCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCA  >  NC_000913/2511165‑2511631
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                             
aTCGCAATTATGAGTGGCGCAAAAACCGTTGGTGGCGTCATGATAATGGCTACCACCGTGGCTGGGATAAGCGTAAAGCGTATGAGCGTGGCTATCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAAccc                                                                                                                                                                                                                                      >  2:428326/1‑237 (MQ=255)
 tggaaatgatgggggggaaaaaacCCGTTGGTGCGTTCTAGAAAATGGCTCCCGCCGTGGCTGGGATAAGCGTAAAGCGTAGGAGCGTGGCTATCGTGAAGGCTGGCGCGATCGGGACGATCATCGGGGAAAAGGCCGGGGTCAGGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAAcgcg                                                                                                                                                                                                                                     <  2:674833/220‑1 (MQ=255)
 tCGCAATTATGAGTGGCGCAAAAACCGTTGGTGGCGTCATGATAATGGCTACCACCGTGGCTGGGATAAGCGTAAAGCGTATGAGCGTGGCTATCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCCTGGGACCCGCCCTTAAACGCGCCCCCCAATGGGGCACAATGCCCGATACGACGCTAGGGCGACTTATCAGGCCTACAAACAGGCACCTTaaagag                                                                                                                                                                                                                                     >  1:316537/1‑235 (MQ=255)
            aGTGGCGCAAAAACCGTTGGTGGCGTCATGATAATGGCTACCACCGTGGCTGGGATAAGCGTAAAGCGTATGAGCGTGGCTATCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGGCATGGGGCCCGCCATTAAACGCACCCGCCAAAGGCGCACAATGCCTGAGGCGACGCCAGTGGGTCTTATACGGCCCACAAACCCGCCCTTAAACGCGTAgggggggaa                                                                                                                                                                                                                          >  1:242963/1‑236 (MQ=255)
             gTGGCGCAAAAACCGTTGGTGGCGTCATGATAATGGCTACCACCGTGGCTGGGATAAGCGTAAAGCGTATGAGCGTGGCTATCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGGCCGCCACTGGCGCACAATGCCGGATCCGACCCTAGGCGGCCTTATACGGCCTACAAACCGGCCCTTTAACCCGAAgccgccgaaa                                                                                                                                                                                                                         >  1:155803/1‑230 (MQ=255)
                 cgcAAAAACCGTTGGTGGCGTCATGATAATGGCTACCACCGTGGCTGGGATAAGCGTAAAGCGTATGAGCGTGGCTATCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCCCAATGCCTGATGCGCCGCCAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACCCGTAGGGCGGGTAAGACg                                                                                                                                                                                                                     >  2:482409/1‑239 (MQ=255)
                 cgcAAAAACCGTTGGTGGCGTCATGATAATGGCTACCACCGTGGCTGGGATAAGCGTAAAGCGTATGAGCGTGGCTATCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCCCTTTAACGCGTAGGGCGGATAAGGCg                                                                                                                                                                                                                     >  2:209638/1‑239 (MQ=255)
                 cgcAAAAACCGTTGGTGGCGTCATGATAATGGCTACCACCGTGGCTGGGATAAGCGTAAAGCGTATGAGCGTGGCTATCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGAGTCTTATCAGGCCTCCAAACCGGCCCTTTAACGCGAAGGGCGGATAAGGCg                                                                                                                                                                                                                     >  2:378887/1‑239 (MQ=255)
                 cgcAAAAACCGTTGGTGGCGTCATGATAATGGCTACCACCGTGGCTGGGATAAGCGTAAAGCGTATGAGCGTGGCTATCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACACGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGCCTTATCAGGCCTACAAACCGGCCCTTTAACGCGCAGGGCGGTTAAgggt                                                                                                                                                                                                                     >  2:610745/1‑237 (MQ=255)
                           gTTGGTGGCGTCATGATAATGGCTACCACCGTGGCTGGGATAAGCGTAAAGCGTATGAGCGTGGCTATCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCGGATGCGACGCTAGTGCGTCTTATCAGGCCTCAAACCCGGCTCTTTAAAGCGTCGGCCGGGTAAGGCGTTTAcgccg                                                                                                                                                                                                            >  2:40209/1‑238 (MQ=255)
                                    gTCATGATAATGGCTACCACCGTGGCTGGGATAAGCGTAAAGCGTATGAGCGTGGCTATCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGTGCACAATGCCTGATGCGACCCTAGTGCGCCTTATCAGGCCTCCAAACCGGCCCTTTACCCCGAAGGGCGGGTAAGGGGGTTAcccccaaccccccaa                                                                                                                                                                                                  >  1:462250/1‑228 (MQ=255)
                                    gTCATGATAATGGCTACCACCGTGGCTGGGATAAGCGTAAAGCGTATGAGCGTGGCTATCGTGAAGGCTGCCGCGATCGTGACGATCATCCCGGAAACGGCCGGGGCCATGGGCACCGCCATTCACCGCGCCCGCCAATGGGCCACAATGCCTGATGCGACCCTAGTGCGTCTTATCAGGCCTCCAAACCCGCCCTTTAACGCCACGGTCGGGTAAGACGTTTaacctcccacccccaa                                                                                                                                                                                                  >  1:269676/1‑224 (MQ=255)
                                                gCTACCACCGTGGCTGGGATAAGCGTAAAGCGTATGAGCGTGGCTATCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGCCGCAAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGCGGGGCGGGTAAGGCGTTTACGCCGCATCCGCCAATAGTGCCACAt                                                                                                                                                                                       >  2:609112/1‑238 (MQ=255)
                                                   accaccGTGGCTGGGATAAGCGTAAAGCGTATGAGCGTGGCTATCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGGGCACAATGCCTGATGCGACGCCATTGCTGTTTATCAGGCCTCACAACCGGCCCTTTAACCCGTAGGGGCGCTAAGGGGTTTACCCCCCATCCCCCAAAAggccccaaaggcc                                                                                                                                                                                   >  1:825457/1‑227 (MQ=255)
                                                     caccGTGGCTGGGATAAGCGTAAAGCGTATGAGCGTGGCTATCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCCCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGGGCCAGATGCGAc                                                                                                                                                                                  >  2:893825/1‑238 (MQ=255)
                                                                      gcgtaaagcgtaTGAGCGTGGCTATCGTGAAGGCTGCCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGCGGAAAATGCCTGATGCGACGCTCGTGCGTCTCATCAGGCCTACAAACCGGCCCTTTAAGCCGTAGGGCGGATAAGGCGTTTACGCCGCACCCGCCCAAAGTGCCAGATGCGCCGCGCATTCAAcaccaaa                                                                                                                                                                 >  1:159602/1‑232 (MQ=255)
                                                                       cgtaaagcgtaTGAGCGTGGCTATCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACCCCGCATCCGCCAATAGTGCCAGATGGGACGCGCATTCAACTACAATcc                                                                                                                                                               >  2:828757/1‑239 (MQ=255)
                                                                           aagcgtaTGAGCGTGGCTATCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGCTAAGGCGTTTACGCCGCATCCGCCAATAGGGCCAGATGCGACGCTCATTCAACTACAATCCCAg                                                                                                                                                            >  2:593546/1‑238 (MQ=255)
                                                                                 aTGAGCGTGGCTATCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCCCATTCAACTAAAATCCCAGCGCCGt                                                                                                                                                      >  2:255429/1‑238 (MQ=255)
                                                                                 aTGAGCGTGGCTATCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCCCTTTAACGCGGAGGGCGGATAAGGCGTTTACGCCGCATCCCCCAATAGTCCCAGATGAGCCGCTCATTCCACCACAACCCCaagcccgg                                                                                                                                                      >  1:783812/1‑231 (MQ=255)
                                                                                        tGGCTATCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGAGCCGTCCccacca                                                                                                                                              >  2:515783/1‑239 (MQ=255)
                                                                                        tGGCTATCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCCCTTTAACGCGTAGGGCGGATAAGGCGGTTACGCCGCATCCGCCAATAGTGCCCGATGCGGCGCCCATTCAACTACAACCCCAGCCCCGTCCccccca                                                                                                                                              >  1:535204/1‑239 (MQ=255)
                                                                                        cgtcTATCGTGAAGGCTGGCACGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGAGTAGGACGGATAAGGCGTTTACGCCGAACCCGCCAATAGAGCCAGAGGCGCCGCTCATTCACTAACAAACCCAACCCCGCccccccaa                                                                                                                                              >  1:966568/4‑235 (MQ=255)
                                                                                            tttcggggagtgggcgccccgcGTGACGATACGTGCGGAAAGGGCCGGGGTGCTTGGCCCCCGCCATGAAGCGCGCACGCAAATGGGGCACAGTGCCTGATGCGACGCTAGTGCGTCCTACCGGGCCTACAACCCGGCCCTTTAACGCGTAGGGCGGATAAGCCGTTTCCGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACcaaca                                                                                                                                           <  1:669973/218‑1 (MQ=255)
                                                                                              tCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTa                                                                                                                                                                                                                                          >  2:69291/1‑141 (MQ=255)
                                                                                                   aaGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCCCCACAAACCAGata                                                                                                                                   >  2:789051/1‑239 (MQ=255)
                                                                                                     ggCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGAAGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCAACCACCATTCAACTACAATACCAGCGCCGTCCCCACCAACAACCAgcaatc                                                                                                                                 >  2:476239/1‑234 (MQ=255)
                                                                                                         cggctgtgtcgtaccagatcgctggAAAAGGGCGGGGGCGTGGGGCCCCGCATTAAACGCCGTCGACCAATGGAGCCAAATCCCTGATGGGCCGGTAGGGCGTCTTACCAGGCCTACAAACCGCCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAg                                                                                                                              <  1:255429/216‑1 (MQ=255)
                                                                                                          gcgcGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGAt                                                                                                                                                                                                                           >  2:598316/1‑144 (MQ=255)
                                                                                                          gcgcGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGCAATTCAgc                                                                                                                             >  2:352024/1‑238 (MQ=255)
                                                                                                               aTCGAGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGGGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACACCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTTAACTACAACCCCAGCCCCGACCCCACAACAAACCAGaaatcaaaaccaaag                                                                                                                       >  1:964733/1‑228 (MQ=255)
                                                                                                                tCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCACCAACCAGCTATTCAGCGCCCCg                                                                                                                       >  2:83015/1‑238 (MQ=255)
                                                                                                                       gccactccggggaaaGGTCGGGGGGGTGGGCGCCCCCAGTTAAACCGGGTCCCCCAATGGAGCAATAGGCCTCAAGCGAGGCTTGTGGGTTTTATCAGGCCAACAAACCGGCTTTTTAACGCGGAGGGCGAAAAAGGCGTTTACGCCGCCTCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGc                                                                                                                 <  1:721545/227‑1 (MQ=255)
                                                                                                                        gcgcagcgggAAAGGGGGGGGGTCAGGGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGcac                                                                                                               <  2:94301/231‑1 (MQ=255)
                                                                                                                        cccgccgctGAAAAGGCCGGGGGTCGTGGCCCCCCCCTTTAACCCCCTCCCCAAAGGGAGCCAAATGCCTGGTGCGCCGCTATGGCGGCTTATCAGGCCTACAAACCGGCCTGTAAAGGCTTAGGGCGGATAAGGCGTTCACCCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGcac                                                                                                               <  1:378887/234‑1 (MQ=255)
                                                                                                                            ccggggAAAGGGCGGGGGTTCTGGGCCCCCCCCTTAAAACCGCCCCCCCAATGGAGCAAAATGCCTGATGCGGCGCTAGGGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACcaca                                                                                                           <  1:893825/235‑1 (MQ=255)
                                                                                                                               gcggcaagagggcgggtgagaggggCCCCGCCAATAACCGCGTCAGGCAATGGAGCCAAACGCCGGGAGCGACGGTAGTGCGTATGACCAGGCCTAAAACCCGGCCCTTTAACGCGTAGGGCGGATAAGGCGTTTCCGCCGCATCCGCCAATAGGGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACcaca                                                                                                           <  1:482409/216‑1 (MQ=255)
                                                                                                                                              ggtccacggccACCGCCTATAAGCGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCTTCTTAACAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCctgtc                                                                                            <  2:316537/228‑1 (MQ=255)
                                                                                                                                                          ccGACATTAAACGGGTCCGCCAATGGATCACAATGCCGGAGTCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGtt                                                                               <  2:640357/239‑1 (MQ=255)
                                                                                                                                                           cGCCATTAAACGCTTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTCTCAGGCCTACAAACCGGCTCTTTAACGCGTAGGTCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCTACGCTCATTCAACTCCAATCCCAGCGCCGCCCCCCCCAACACCCCAACATTCACCCCCCCAGCCAGCCCCCCAATAACCCACCCTGTCGGTTTTCCCCGttt                                                                              >  1:486632/1‑239 (MQ=255)
                                                                                                                                                              cATTAAACGCGGCCGACAATGGAGCAAAATGCCTGATGGGACGTTAGTGCGTCTTATCAGGCCTAAAAACCGGCTCTTTAACGGGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCt                                                                           <  2:783812/239‑1 (MQ=255)
                                                                                                                                                                       cgcgTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCATATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGCCGGTTTTACCCGGTTGCTGTTcccc                                                                    >  2:699762/1‑239 (MQ=255)
                                                                                                                                                                       cgcgTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGc                                                                                                 >  2:871534/1‑210 (MQ=255)
                                                                                                                                                                           gTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAg                                                                  >  2:1003611/1‑238 (MQ=255)
                                                                                                                                                                           gTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATACTCAGCGCCACCCCCAGCCCCACAAACACCCAGCATCGCTCTTTTACCAGTTTGCTGTTCACCaa                                                                  >  1:824403/1‑237 (MQ=39)
                                                                                                                                                                             tCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATAtt                                                                                                                                 <  2:356409/174‑1 (MQ=255)
                                                                                                                                                                             tCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATAtt                                                                                                                                 >  1:356409/1‑174 (MQ=255)
                                                                                                                                                                                               tGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTCCAATCCCAGCGCCGTCCCCACCACCAACCAGATATTCAGCGCCCCGACCAGCCCCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTTGTCACCAAATCGCCCacaaaatt                                                   >  1:934013/1‑227 (MQ=255)
                                                                                                                                                                                               tGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGGTTTACGCGTTTGCTGTTCACCAGATCGCCCATACACtt                                                   >  2:938677/1‑234 (MQ=255)
                                                                                                                                                                                               tGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGCCTGTTTTACGCGTTTGCTGTTCACCAAATctgtcccttataaacat                                               >  2:483688/1‑222 (MQ=255)
                                                                                                                                                                                               tGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGAGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGAGTTTGCTGTTCACCAAATAGCCCATCACCTTgacga                                              >  1:583513/1‑235 (MQ=255)
                                                                                                                                                                                                      tGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGt                                       >  2:917029/1‑239 (MQ=255)
                                                                                                                                                                                                       ccggtcctggttggctttacccggCCAACAACCCGGTCATTTAACGGGTAGGGGGGACAGGGCTTTCTCCCAGCCCCCGCCAATGGGGCCAAATGCGGCTATCATTCAACAACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGGGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCGCCAGATCGCCCATCAACTTGCTGTCACTGGTg                                      <  1:515783/217‑1 (MQ=255)
                                                                                                                                                                                                           cGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAACACCACAATCCCCCAGCCTGTCTGTTTTCCGCGTTTTGTGTTCACCATAACGCCCATCAACTTCCTGTCACTGGTGaaaa                                  >  1:643085/1‑239 (MQ=255)
                                                                                                                                                                                                              tAGTGCGTCTTAACAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCGGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATca                               <  2:462250/239‑1 (MQ=255)
                                                                                                                                                                                                              tAGGGCGTTTTAACAGGCCTAAAAACCGGCTCTGTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATc                                <  2:825457/238‑1 (MQ=255)
                                                                                                                                                                                                               aGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCACACCCACGACCAGCACCCCAATCACCCAACCTGCCTGTTTTACGCTTTTGCTGTTCACCAATACCCCCCTCAACTTTCTCTCACTGGGAaaaaccc                               >  1:565038/1‑235 (MQ=255)
                                                                                                                                                                                                                     tCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGCCTGTTTTACGCGTTGGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCACCAGTgg                        >  2:962074/1‑239 (MQ=255)
                                                                                                                                                                                                                        tagcAGGCCAAAAAACCGGCTCTTTAACGCGTAGGGCGGATAGGGCTTTTCCGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAAc                     <  2:484773/236‑1 (MQ=255)
                                                                                                                                                                                                                        tATCAGGCCTACAACCCGGCTCTTTAACGCGTAGGGGGGATAAGGCGTTTACCCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAAc                     <  2:416271/239‑1 (MQ=255)
                                                                                                                                                                                                                        tATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCt                                                                           >  1:460700/1‑185 (MQ=255)
                                                                                                                                                                                                                        tATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCt                                                                           <  2:460700/185‑1 (MQ=255)
                                                                                                                                                                                                                        tATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCCCTGGTGAAAATc                                >  1:876885/1‑228 (MQ=255)
                                                                                                                                                                                                                        tATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATc                                <  2:876885/228‑1 (MQ=255)
                                                                                                                                                                                                                          tCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACTCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAAAACGCCCAACACCCTGCCTGCACCGGTGAAAATCAGCCCTGGaaac                    >  1:411001/1‑236 (MQ=255)
                                                                                                                                                                                                                                   cAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGGGGAACCAGCGCCaga           >  2:830883/1‑238 (MQ=255)
                                                                                                                                                                                                                                      aCCGGCTCTTTAACGTGTAGGGCGGATAAGGCGTTTCGTCCGCACCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACcaa                                                                                                                                             <  1:183977/105‑1 (MQ=255)
                                                                                                                                                                                                                                        cGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATGGCCCCTCAACTTCCTGTCACTGGTGCAAATCACCAGGGGAACCAgacccaagacgata     >  1:613611/1‑226 (MQ=255)
                                                                                                                                                                                                                                        cGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAAt                                 <  2:120468/211‑1 (MQ=255)
                                                                                                                                                                                                                                        cGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAAt                                 >  1:120468/1‑211 (MQ=255)
                                                                                                                                                                                                                                         ggCTCTTTAACGCGTAGGGCGGATAGGGCGTTGACGCCGCATCCGACAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATAc    <  2:159602/239‑1 (MQ=255)
                                                                                                                                                                                                                                            tctTAAACGCGGAGGGCGGATAAGGCGTTGACGCCGCCACCGCCAATAGGGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCa  <  2:934013/238‑1 (MQ=255)
                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                             
ATCGCAATTATGAGTGGCGCAAAAACCGTTGGTGGCGTCATGATAATGGCTACCACCGTGGCTGGGATAAGCGTAAAGCGTATGAGCGTGGCTATCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTTAACGCGTAGGGCGGATAAGGCGTTTACGCCGCATCCGCCAATAGTGCCAGATGCGACGCTCATTCAACTACAATCCCAGCGCCGTCCCCACCAACAACCAGATATTCAGCGCCACGACCAGCACCACAATCACCCAGCCTGTCTGTTTTACGCGTTTGCTGTTCACCAGATCGCCCATCAACTTGCTGTCACTGGTGAAAATCAGCAGTGGAACCAGCGCCAGAGCGATACCA  >  NC_000913/2511165‑2511631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: