| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,596,612 | T→C | 13.1% | I42V (ATA→GTA) | ypfJ ← | putative neutral zinc metallopeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,596,612 | 0 | T | C | 13.1% | 166.9 / 2.2 | 80 | I42V (ATA→GTA) | ypfJ | putative neutral zinc metallopeptidase |
| Reads supporting (aligned to +/- strand): ref base T (31/34); new base C (3/8); total (34/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.27e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
TCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTC‑GAACTGTTG‑TCCCCAGGTGTCTTCCGTG‑GTTGCCAGAATCACCGAGGTGAATTT‑TGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGCCAACGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCAT > NC_000913/2596387‑2596842 | tCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTC‑GAACTGTTG‑TCCCCAGGTGTCTTCCGTG‑GTTGCCAGAATCACCGAGGTGAATTT‑TGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTAT‑CAGTAAAATCAg > 2:214558/1‑238 (MQ=255) cATTCCACGGTACATTACCAGCTTCGGTTGCGGTTAGGTCTTTCCCATCTTCTC‑GACCTTTTG‑TCCCCAGGTGTCCTCCGGG‑GTTGCCATAATCACCGAGGTGAATTT‑TGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTAT‑CAGTAAAATCAGcc < 2:482710/239‑1 (MQ=255) tCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTC‑GAACTGTTG‑TCCCCAGGTGTCTTCCGTG‑GTTGCCAGAATCACCGAGGTGAATTT‑TGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTTCAACCAGCCCGACTAT‑CAGTAAAATCAGCCCg > 2:760908/1‑238 (MQ=255) cGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTC‑GAACTGTTG‑TCCCCAGGTGTCTTCCGTG‑GTTGCCAGAATCACCGAGGTGAATTT‑TGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCACAACCATAGTAGCCTGCAACAACCACGCCACT‑CAGTAAAATCAACCCCCCttt > 1:781419/1‑239 (MQ=255) tACATGACCAGCTTCGGTTGCTGATAGGTCTAACCCATCTTCTC‑GAACTGTTG‑TCCCCAGGTGTCTTCCGTG‑GTTGCCAGAATCACCGAGGTGAATTT‑TGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCAGGTCAAATCAACACCCAAGTGGCCGGCAACCAGAACGACTGC‑CAGTAAAATCAGCCCACCTTTAc > 2:75930/1‑238 (MQ=255) cATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTC‑GAACTGTTG‑TCCCCAGGTGTCTTCCGTG‑GTTGCCAGAATCACCGAGGTGAATTT‑TGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCTAAGTAGCCTGGAACCAGCACGACTAT‑CAGTAAAATCAGCCGCCCTTTAcc > 2:148404/1‑237 (MQ=255) cAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTC‑GAACTGTTG‑TCCCCAGGTGTCTTCCGTG‑GTTGCCAGAATCACCGAGGTGAATTT‑TGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCa > 2:777103/1‑239 (MQ=255) gCTTCGGTTGCTGATAGGTCTTACCCATCTTCTC‑GAACTGTTG‑TCCCCAGGTGTCTTCCGTG‑GTTGCCAGAATCACCGAGGTGAATTT‑TGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATCATAGCCCGCAACCAGC‑CGCCCCTACAGTAAAATCACCCCCCCTTTCCCGCCGGGCAgg > 1:186878/1‑239 (MQ=255) gCTTCGGTTGCTGATAGGTCTTACCCATCTTCTC‑GAACTGTTG‑TCCCCAGGTGTCTTCCGTG‑GTTGCCAGAATCACCGAGGTGAATTT‑TGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAgg > 2:798322/1‑239 (MQ=255) gCTTCGGTTGCTGATAGGTCTTACCCATCTTCTC‑GAACTGTTG‑TCCCCAGGTGTCTTCCGTG‑GTTGCCAGAATCACCGAGGTGAATTT‑TGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAg > 2:867675/1‑238 (MQ=255) gCTTCGGTTGCTGATAGGTCTTACCCATCTTCTC‑GAACTGTTG‑TCCCCAGGTGTCTTCCGTG‑GTTGCCAGAATCACCGAGGTGAATTT‑TGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCGGCAACAAGCACGACTAC‑CGGAAAAAACACCCCCCCTTTACCCCTTggaggg > 1:931933/1‑235 (MQ=255) gCTTCGGTTGCTGATAGGTCTTACCCATCTTCTC‑GAACTGTTG‑TCCCCAGGTGTCTTCCGTG‑GTTGCCAGAATCACCGAGGTGAATTT‑TGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACAACATAGTACCCCGCAACCAGCACGACTAC‑CAGGAAAAACAGACCCCCTTTACCGCTTGGCAgg > 1:148332/1‑239 (MQ=255) cTTCGGTTGCTGATAGGTCTTACCCATCTTCTC‑GAACTGTTG‑TCCCCAGGTGTCTTCCGTG‑GTTGCCAGAATCACCGAGGTGAATTT‑TGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACCCCATAGAAGCCTGCATCCAGCACGAATAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGCCAGGc > 2:910630/1‑239 (MQ=255) ttCGGTTGCTGATAGGTCTTACCCATCTTCTC‑GAACTGTTG‑TCCCCAGGTTTCTTCCGTG‑GTTGCCAGAATCACCGAGGTGAATTT‑TGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCg < 2:275732/239‑1 (MQ=255) ttttacgggaccgggTTTTTCCCATTTTCTC‑GAACTTTTT‑TCCCCAGGTTTCTTCCGGG‑GTTGCCAGAATCACCGAGGTGAATTT‑TGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCGGCAACCAGCACGACTAC‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCg < 1:970365/225‑1 (MQ=255) gATGGGTTTTACCCATCTTTGC‑GAATTGTTG‑TCCCCGGGGTTCTTCCGTG‑GTGGCCAGAATCACGGGGGTGATTTT‑TGCGGCTTCGTCTTCATTGGGGCCAATTGGCCGCGTTGATTTGTGTTGGGAACCCGGCTGCCCGGTCATCAACCCGGTTAGATCAACACCATAGTAGCCTGCAACCAGCACGACTAC‑CAGTAAAACCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGa < 1:162465/239‑1 (MQ=255) ttgggTTTTTCCCTTCTTCTC‑GAACTGGTG‑TCCCACGGTTTCTTCCGGT‑GTTGCCAGAATCACCGGGTTGAATTT‑TGCGGCTTCGTCTTCATTTGGGCTATTTGCCCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGAc < 1:876207/236‑1 (MQ=255) aagcgttttcccctttttctC‑GAATTGTTG‑TCCCCGGGTTCATTCCGGT‑GGTGCCATAATCACGGAGGTTAATTT‑TGCGGCTTCGGCTTCATTGGGGCTAATTGACCGCGTGGATTGTTTTTGGGAAACCGGCTCCCCGGTCACCAACCCGGTTAAATCACCACCATAGTAGCCTGCAACCAGCACGACTAT‑CAGTAAAACCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGAc < 1:117965/223‑1 (MQ=255) ttggTCTTTCCCTTC‑TCTCTGAATTGTTC‑TCCCAGGGTTGTTTCCGTG‑GTTGCCTGATTGACCG‑GGTGTATTTGTGCGGCTTCGTCTTCATTTGGCTAAATTGTCCGCGTTGATTGTGGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATATTAGCCTGCAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCATTTACCGCTTGGCAGGCGAAAACCGGGAc < 1:469330/236‑1 (MQ=255) aGGTTTTACCCATTTTCCC‑GAACTGTTG‑TCCCAAGGTGTCTTCCGTG‑GTTGCCAGAATCACCGAGGTGAATTT‑TGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCg < 2:931933/239‑1 (MQ=255) aTCTTCCC‑AAACTGTTG‑TCCCCAGGTGTCTCCCGTG‑GTTGCCAGAATCACCGAGGTGAATTT‑TGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGAAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACCCCATAGTAGCCTGCAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATgg < 2:148332/239‑1 (MQ=255) tcttctC‑GAACTGTTG‑TCCCCAGGTGTCTTCCGTG‑GTTGCCAGAATCACCGAGGTGAATTT‑TGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGAcctgtcccttataca > 2:970365/1‑226 (MQ=255) cttctC‑GAACTGTTG‑TCCCCAGGTGTCTTCCGTG‑GTTGCCAGAATCACCGAGGTGAATTT‑TGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGCCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAAACGGGACCGCCCAAAGATGGa > 2:712548/1‑238 (MQ=255) gttactcgtgtgttgtTCCCCAGGTGTTGTGCGTG‑GGTCCCGTAATCCCCGGGGCGAATTT‑GGCGCATTCGTCGGCATTGGGGCAAAGTGCCCGCTTGTATTGTTGTTGCGAAACGGGCTGCCGGGCAATCAACCCGGTTAAATAAACCCCATATTAGCCGGTACCCTGCACGACTAC‑CAGTAAAATCAGCCCGCCTTTACCGCTGGGCAGGCGAAAACCGGGACCGCCCATAGATGGa < 1:213596/229‑1 (MQ=255) gttg‑tCCCCAGGTGTCTTCCGTG‑GTTGCCAGAATCACCGAGGTGAATTT‑TGCGGCTTCGTCTTCATTTGGGCTAATTGACAGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGCGGAAAACCGGGACCGCCCATAGAGGGacacccgcagccg > 2:630083/1‑228 (MQ=255) ttg‑tCCCCAGGTGTCTTCCGTG‑GTTGCCAGAATCACCGAGGTGAATTT‑TGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGCTTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTACATCAACACCATAGTAGCCTGCAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATGGATGGCACACCAGAGCTGt > 2:531442/1‑239 (MQ=255) cccgtggttCTTCCGGG‑TTTGTCATAATCCCCGAGGGTAATTT‑TTGGGTTTCTGCTGGCTTTGGGATAATGGGCCGCGTTGTCTGTTGGTTGGAAACCGGCGGCCCGGTCATCAACCCGGTAAAACCAACACCCTAGCAGCCTGAAACCAGCACGACTAA‑CAGTAAAATCATCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTgcgc < 1:760908/231‑1 (MQ=255) cccagtgtTTTGCGTGTGTTTCCAACATCACCGAGTTGAATTT‑TGCGGTGTCTTTTTAATTTGGGTTAATTGACCGCTGATTTTTTTGTTGGGAAACCGGTTGCCCGGTCACCAACCCGGTTAAATCAACACATTAGTCGCCTTCAACCAGCACGACTAC‑CAGTAAAATCAGCCCGCCTTGACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTgcgc < 1:777103/235‑1 (MQ=255) gaGGTGTCTGCCGTG‑GTTGCCAGAATCACCGAGGTGAATTT‑TGCGGCTTCGTGTTCTTTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCACCCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCt < 2:545421/238‑1 (MQ=255) gtgtCTTCCGTG‑GTTGCCAGAATCACCGAGGTGAATTT‑TGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTAT‑CCGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCAAAAACGAACCACCAAAGCGGTTGCGCCTg > 1:15176/1‑237 (MQ=255) tCTTCCGTG‑GTTGCCAGAATCACCGAGGTGAATTT‑TGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCCCGACTAT‑CAGTAAAATCAGCCCCCATTTACCCCTTGGCAGGGCAAAACCGGGACCGCCCCAAAATGGCCCACCCAAGCGGTTCCGCC‑GTCtttc > 1:745591/1‑237 (MQ=255) tCTTCCGTG‑GTTGCCAGAATCACCGAGGTGAATTT‑TGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAA‑CCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACAACCAAAGCTGTTGCGCCTGTCTTCa > 2:779555/1‑239 (MQ=255) ttCCGTG‑GTTGCCAGAATCACCGAGGTGAATTT‑TGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCaa > 2:331532/1‑239 (MQ=255) g‑gTTGCCAGAATCACCGAGGTGAATTT‑TGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGt < 2:691238/239‑1 (MQ=255) gTTGCCAGAATCCCCGAGGTGAATTT‑TGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGt < 2:186878/238‑1 (MQ=255) aGAATCACCGAGGTGAATTT‑TGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑ttt > 2:485564/1‑238 (MQ=255) aTCACCGAGGTGAATTT‑TGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACCCCATAGTAGCCTGCAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACg < 2:781419/239‑1 (MQ=255) cgtggttatttt‑tGTGGTTTCGTCTTCATTTGGGCTAATTGCCCGCGTTGATTGTTTTTGGGAAACCGGTCGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGCCTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACGTCGcc < 1:183924/230‑1 (MQ=255) gttttttggcgttttgtttttttttGGTGTAATTGCCCGCGTTTATTGTTTTTGGGAACCCGGCTGCCCGGTCATCCACCGGGTTAAACCAACCCCATAGTAGCCTGCAACCAGCCCGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGcc < 1:867675/217‑1 (MQ=255) aaTTT‑TGCGGCTTCGTTTTAATTGGGGCTAATGGGCCGGGTTGATTGTTGTGGGGAACCCGGCTGCCCGGTCACCAACCCGGTTAAACCAACCCCATAGTAGCCTGCAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGct < 2:764175/238‑2 (MQ=255) aaTTT‑TGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAAACCGGTTAAATCAACACCCTAGTAGCCTGCAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGGGAAACACGGGACCGCCCCAAGATGGACCACCAGAGCTGTTCGGACAGGTTTCAAAATTGCAACTTTTCAC‑TCGCCCTTGCCa > 1:484414/1‑239 (MQ=255) atttgtcGGGTTCGTGTTCTTTTGGGTATATTGACCGGGTTGAGTGTTTTTGGGAACCCGGCCGCCCGGTCACAAACCCGGTTAAACCAACCCCAAAGTAGCCTGCACCCAGAACGACTAC‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGCCaa < 1:331532/233‑1 (MQ=255) tggCGGCTTCGTCTTCATTTGGGCTAATTGACCGGGTTGATTGTTTTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACCCCATAGTAGCCTGCAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGCCAACGc < 2:745591/237‑1 (MQ=255) gggttttcttttattttGGGTTAATGTGCCGCTTTGATTTGTGGTGGAAACCCGGCGCCCCGGCAACCAACCCGGTTAAAACAACCCCATATTAGCCGGCACCCAGCACGACTAC‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGCCAACGCATa < 1:648260/225‑1 (MQ=255) tgtttCGTCTTTATTTGGGTGATTTGGCCGGGTTATTCTTTGTTGGGAACGCGCTGGCCCGGTCATCACCGCGTTTAAATCCACCCCCTAGGGACCGCCAACCAGCACGACTAT‑CATTAAAACCAGCCCCCCTTTACCACTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTTTCAC‑TTTCACGTCGCCCTTGCCAACGCATa < 1:630083/235‑1 (MQ=255) ttttcttatttgtttGGGCTATTGTCCCGCGTTGTTTGTTGTTGGAAACCCGGCGGCCCGGTCATCACCCCGGTAAAACCAACACCTTGGTAGCCAGCAACCAGCACGATTAG‑CAGTAAAAGCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGCCAACGCATAGa < 1:205793/227‑1 (MQ=255) gtcgggttttCCTTTGGGTTAATTTCCCCTGTTGATTGTTTGTGGGAACCTCGCTGCCCGGCAACAAACCCGGTTAAATCCACCCGATTGTACCCTGCAACCGGCCCGACTCT‑CAGTAAATCCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGGCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGCCAACGCATAGa < 1:681130/231‑1 (MQ=255) tttcttcttcGTTGGGGCTAAGTGCCCGCTTTGATTTTTTTGGCGAAACCGGTGTCCCGTACATCACCACGGTTAAACACACCCCTGTTTACCCTGCAACCATCACACCTAC‑CAGTAAAATCACCCCTCCTTTACCGCTTTGCAGGCGAATACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGCCAACGCATAGa < 1:35006/237‑1 (MQ=255) tttCGTTTTCATTTGGGCTAATTGCCCGGGTTGATTGGTTTTGGGAACCCGGCTACCCGGTCCACAACCCTGTAAAATCAACCCCATAGTAGCCGGCAACCAGCCGGACTAG‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGCCAACGCATAGa < 1:214558/237‑1 (MQ=255) ctttctttttttttGGGCTAATGGCCCGGTGTGATTGTTTGTGGGAACCCGGCTGCCTGGTCACCAACCCGTGTCAATCAACCCCATAGTAGCCGGCAACCAGCACGAATAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGCCAACGCATAGAt < 1:944031/228‑1 (MQ=255) cTTCGTCCTCATGTGGGCTAATTGACCGGGTTGATGGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACGCCATTGTAGCCTGCAACCAGCACGACTAT‑CAGTAAAAGCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGCCAACGCATAGa < 2:199126/238‑1 (MQ=255) cctCTTTATTTGGGCAAATTTCCCGCGTTTATTGTTTTTGGAAAACCGGCCTCCCGGTCA‑CAAACCGGTTAAAACAACCCCAAAGTCGCCTGCCACCAGACCGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGCCAACGCATAGATACCt < 1:779555/237‑1 (MQ=255) gTCTTCATTTGGGCTAATTGACCGAGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGCCAACGCATAGATACCt < 2:302613/239‑1 (MQ=255) cTTCATTTGGGTTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACCCCATAGTAGCCTGCAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGCCAACGCATAGATACCTca < 2:466124/239‑1 (MQ=255) cTTCATTTGGGCTAATTGACCGCGTTGATTGTGTTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGCCAACGCATAGATACCTca < 2:484414/239‑1 (MQ=255) cttttGGGCTAGTGTGCCGGGTTGATTGGTGTGGAGAAACCGGCGGCCCGGTACTCACCCAGGTAAAAGCAACACTATAGTCGCCGGCAACCAGCACAACTAT‑GAATAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGCCAACGCATAGATACCTcaaca < 1:373139/237‑1 (MQ=255) cATTTGGGCTAATTGACCTCGTTGATTGTTGGTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACCCCATAGTAGCCTGCAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGCCAACGCATAGATACCTcaaca < 2:688360/239‑1 (MQ=255) ggCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGCCAACGCCACGATACCTcaacaactg > 2:681130/1‑234 (MQ=255) tAATTGACCGGGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGCCAACGCATAGATACCTCAACAATATATTCa < 2:15176/239‑1 (MQ=255) aaTTGACCGGGTTGAGTGTTGTTGGGAACCCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTAT‑CAGTAAAACCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGCCAACGCATAGATACCTCAACAATATATTCa < 2:217813/238‑1 (MQ=255) cgggTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGCCAACGCATAGATACCTCAACAATATATTCATTATAGaa < 2:928183/236‑1 (MQ=255) cgcgTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGCCAACGCATAGATACCTCAACAATATATTCATTATAGaa < 1:51223/239‑1 (MQ=255) cgcgTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGCCAACGCATAGATACCTCAACAATATATTCATTATAGaa > 2:51223/1‑239 (MQ=255) gggttgttTTTTTTTGGGAAACCGGCTGCCCGGTCAACAACCCGGTTAAAACACCCCCAAGGTAGCCTGCAACCAGCCCGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGCCAACGCATAGATACCTCAACAATATATTCATTATAGaaa < 1:485564/237‑1 (MQ=255) ggAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGCCAACGCATAGATACCTCAACAATATTTTCATTATAGAAATGATCGTAGGTGGTTc > 2:620267/1‑239 (MQ=255) ccGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTgcgc < 2:593141/143‑1 (MQ=255) ccGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTgcgc > 1:593141/1‑143 (MQ=255) cccGGTTAAACCAACCCCATAGTACCCTGCAACCAGCACGACTAT‑CAGTAAAACCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGCCAACGc < 1:45851/166‑1 (MQ=255) tCAACACCATAGTAGCCTGCAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGCCAACGCATAGATACCt > 2:45851/1‑166 (MQ=255) atattttcccccacccaagccGACTGG‑CAGTAAAGTCGACCCGGTTTTCCCGCTTGGCGGGCGAAAACCGGGGCCGTCCGAAGATGGACCCCCAGAGCATTTGCGGCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGACAACGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCggagg < 1:620267/219‑1 (MQ=255) gcgCCTGCAACCAGCCGTACTAC‑CAGTAAAATCATCCCGCCTTTACCGGTTGGCAGGCGAAATCCGGGACCGCCCATAGAAGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGCCAACGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAAt < 1:829507/236‑1 (MQ=255) gCCTGCAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGCCAACGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGGGGAAGATTACCCCAGGAAAGAGGGATGAAATGGGGGGGGAATGAg > 1:892537/1‑239 (MQ=255) tGCAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGCCAACGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGCGGAAAATTACCACAGGAAAGCGGGATGAAATGGCGGGGGAAAGAACgg > 1:530521/1‑238 (MQ=255) tGCAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGCCAACGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACCGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGt > 2:558788/1‑239 (MQ=255) tGCAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGCCAACGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGt > 2:829507/1‑239 (MQ=255) cAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGcc < 2:20912/132‑1 (MQ=255) cAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGCCAACGCATAGATACCTCAACAATATATTCATTATAGCAATGATCGTAGGGGGTTAAGCGGAAGATTACCACAGGAAAGCGGGATGAAAGGGGGGGGGAAAAGGCGTTc > 1:539636/1‑239 (MQ=255) cAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGCCTTCAACATTGTCAC‑TTTCACGTCTCCCTTGcc > 1:20912/1‑132 (MQ=255) aCCAGCTCGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTGGGCAGGCGAAAACCGGGACCGGCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGCCAACGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCaa < 2:539636/239‑1 (MQ=255) cACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGCCAACGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAACTGGCGGAGGAATGAGCGTtccatccag > 2:883499/1‑238 (MQ=255) t‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCTCCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGCCAACGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGtt < 1:561298/170‑1 (MQ=255) t‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGCCAACGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGtt > 2:561298/1‑170 (MQ=255) | TCATTCCACGGTACATGACCAGCTTCGGTTGCTGATAGGTCTTACCCATCTTCTC‑GAACTGTTG‑TCCCCAGGTGTCTTCCGTG‑GTTGCCAGAATCACCGAGGTGAATTT‑TGCGGCTTCGTCTTCATTTGGGCTAATTGACCGCGTTGATTGTTGTTGGGAAACCGGCTGCCCGGTCATCAACCCGGTTAAATCAACACCATAGTAGCCTGCAACCAGCACGACTAT‑CAGTAAAATCAGCCCGCCTTTACCGCTTGGCAGGCGAAAACCGGGACCGCCCATAGATGGACCACCAGAGCTGTTGCGCCTGTCTTCAACATTGTCAC‑TTTCACGTCGCCCTTGCCAACGCATAGATACCTCAACAATATATTCATTATAGAAATGATCGTAGGTGGTTCAGAGGAAGATTACCACAGGAAAGCGGGATGAAATGGCGGAGGAATGAGCGTTCAATGCAT > NC_000913/2596387‑2596842 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |