| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,277,446 | G→A | 16.7% | E38K (GAA→AAA) | yhaV → | toxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,277,446 | 0 | G | A | 16.7% | 164.5 / 12.9 | 81 | E38K (GAA→AAA) | yhaV | toxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system |
| Reads supporting (aligned to +/- strand): ref base G (32/34); new base A (2/12); total (34/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.44e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
TTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGG‑TTAATGGTTGGGCG‑CTATATG‑CTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCAT‑GGCAAGTCGTTAGGCTCTGGGAAAAATAAAGAC‑TGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACA > NC_000913/3277213‑3277680 | tttCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGG‑TTAATGGTTGGGCG‑CTATATG‑CTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAAt > 2:240794/1‑239 (MQ=255) atatCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGG‑GTAATGGTTGGGCG‑CTATATG‑CTCATCCCTGTTTTCAGGAAC‑CCTACACCGCTTTAGTTGCCGAAGGCGAGCCTTTAAAGGGAAAAGATCCTGAAAATAAACAGaaaaaa > 1:516769/1‑238 (MQ=255) tCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGG‑TTAATGGTTGGGCG‑CTATATG‑CTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGGGAAAAgcc > 2:429919/1‑238 (MQ=255) tCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGG‑TTAATGGTTGGGCG‑CTATATG‑CTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAATCGAAACATTAAAGGGAAACAATCCTAAAAATAAT‑AAAGaaaaaacg > 1:514417/1‑234 (MQ=255) cAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAACGGG‑GTAATGGTTGGGCG‑CTATATG‑CCCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCGTGAAAATTATCAGAGAAAAgctgac > 2:799868/1‑235 (MQ=255) cccGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGG‑TTAATGGTTGGGCG‑CTATATG‑CTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGCGCCATTAAAGGGAAAAGAACCTGAAAATTATCAGAGAAAACCCG‑CACaaaagta > 1:423071/1‑235 (MQ=255) cccGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGG‑TTAATGGTTGGGCG‑CTATATG‑CTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAggt > 2:685468/1‑237 (MQ=255) cccGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGG‑TTAATGGTTGGGCG‑CTATATG‑CTCATCCCTGTTTTCAGGAAA‑CCTACGACACTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGtt > 2:733799/1‑239 (MQ=255) cccGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGCCATGGACGTCAACATTGATGATGTGATTGGCGACGACGACTAATGGATTTTCCACAAAGGG‑TTAATGGTTGGGCG‑CTATATG‑CTCATCACTGTTTTCAGGAAA‑CCGACGACGCTTTACTTGCCGAAGTCAAGACGTTAAAGGGAAAGAATCATGAAAATTATCAAAGAAAAGCCGCCGCAAAttt > 1:58098/1‑239 (MQ=255) cAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGG‑TTAATGGTTGGGCG‑CTATATG‑CTCATCCCTGTTTTCAGGAAA‑CTTACGAAGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAACAGAACCTGAAAATTACCAGAGAAAAGCCGCCCAAAAGAAATTg > 1:258093/1‑239 (MQ=255) aaaaacCTCGTCCATTAAACATTCAACAAGAAAAGAAACTTGCCGCTGGCAGGGACGTCAACATTGATGATGAGTTTGGCGCCGACGAATAATGAATTTTCCAAAAAGGT‑TTAATGGTTGGGCG‑CTATATG‑CTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTgg < 2:514417/239‑1 (MQ=255) aaaaaaCTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGG‑TTAATGGTTGGGCG‑CTATATG‑CTCATCCCTGTTTTCAGGAAA‑CTTACGACGCTTTAGTTGCCGAAGTCGAGCACTTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCCCCACAAAGAAATTgg > 1:171502/1‑239 (MQ=255) aaatCTCCTCTATTAAACATGCAAAAAGGAAAGAACATTGTCGCTGGAATGGACGTAAATATGGATGATGAGGTTGGATACGACGAATAATGGATTTTCCACAAAGGT‑TTAAGGGTTGGGCG‑CTATATG‑CTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGACACATTAAAGGGAAAAGATCCGGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCg < 1:505708/239‑1 (MQ=255) aaagcgtgTCCTTTCAACATTAAACAAGGAAAGAAGTGTGTCGGTGGCAGGGACGAACACATATATGATGAGATTGGGGGCGACTAATAATGGATTTTCCACAAAGGG‑TTAATGGTTGGGCG‑CTATATG‑CTCATCCCTTTTTTAAGGAAA‑CCTACGACGTTTTAGTTGCCGATGTCGAGACATTAAAGGAAAAAGATCCGGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCg < 2:558725/232‑1 (MQ=255) aaCTCGTCCATTAAACATTCAACAAGGAAAAAACCTTGGCGCTGGCATGGACGTCAACAGTGATGATGAGATTGGCGACGAAGAATAATGGATTTTCCACAAAGGG‑TTAATGGTTGGGCG‑CTATATG‑CTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGt < 2:1742/239‑1 (MQ=255) aaCTCGTCCATTAAACATTAAACAAGGAAAGAAACTTGTCGCTGGCAGGGACGTCAAAATTGAGGAGGAGATTGGCGACGACAAATAATGGATTTTCAAAAAAGGG‑TTAATGGTTGGGCG‑CTATATG‑CTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATAAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCTGt < 2:171502/239‑1 (MQ=255) cGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGG‑TTAATGGTTGGGCG‑CTATATG‑CTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCCCAAAGTTATTGGCGGTA‑GTc > 2:27557/1‑239 (MQ=255) ctctttaaacattaaaaaGGGAAAAAAACGTTCCCGTGGAGTGGACTTCAAATTTGAGGGTGGGTTTGGGGACGAGAAATAAGGGATTTTCAAAAAAGGG‑TTAATGGTTGGGCG‑TTATAGG‑CTCATCCCTGTTTAGAGGAAA‑CCTACGACGCTTTAGTCGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAAAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCa < 1:27557/232‑1 (MQ=255) tccagaaaGGAAAAAAGTGTGCCGCTGGCATGGACGTCAACATTGATGATGAAATGGGCGACGACGAATAATGGATTTTCAAAAAAGGG‑TTAAGGGTTGGGCG‑CTATATG‑CTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTAAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTg < 2:423071/233‑1 (MQ=255) tacaacaaGGAAAGAAACTTGTCGCTGGCATGGCCTCAAACATTGATGATGAGATTGGCAACGACGAATAATGGATTTTCCACAAAGGG‑TTAATGGTTGGGCG‑CTATAGC‑CTCATCCCTGTTTTCAGGAAC‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTg < 2:725782/237‑1 (MQ=255) tcaacaAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGG‑TTAATGGTTGGGCG‑CTATATG‑CTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑AGCCA‑TAAAATGATTga > 2:777067/1‑239 (MQ=255) tcaacaAGGAAAGAAACTTGTCGCTGGCATGGACGTAAACATTGATGATGGATTTGGCGACGAAGAATAATGGATTTCCCAAAAAGGG‑TTAAGGGTTGGGCG‑TTAATTG‑CTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTga < 2:482008/239‑1 (MQ=255) caaaagggAAAAAATCTTGTCGTGTGCATTGCAGGCAAAATGGAGGAGGAGATTGGCGAAGACGAATAATGGTTTTTCCACAAAGGG‑TTAATGGTTGGGCG‑CTATATG‑CTCATCCCTGTTTTCAGGAAA‑CCTACGAGGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTgag < 2:1004408/233‑1 (MQ=255) acaaGGAAAGAAACTGGTCGCTGGTGTGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTCCCACAAAGGG‑TTAATGGTTGGGCG‑CTATATG‑CTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGAAAAAGATCCTAAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTgagga < 2:454325/239‑1 (MQ=255) aaGGAAAAAAACTTGTCGGGGGCATGGACGCAAAAATTGAGGAGGAGGTGGGGCAAGAGAAATAATGGATTTTCCAAAAAGGG‑TTAATGGTTGGGTG‑CTTTATG‑CTCTCCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCATGAAAATTATAAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTGAGGAGc < 1:240794/239‑1 (MQ=255) ggAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGG‑TTAATGGTTGGGCG‑CTATATG‑CTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCC‑TAAAATGATTTAGGGGcct > 2:67265/1‑237 (MQ=255) ggAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGG‑TTAATGGTTGGGCG‑CTATATG‑CTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTACA‑TAAAATGATTTAGGGGCat > 2:752415/1‑239 (MQ=255) cctgggtggtggatgtgACGTCAACATTGGTTATGGAGTGGGCGACGACGAATAATTGATTTTCCAAAAAGGG‑TTAATGGTTGGGAG‑CTATATG‑CTCACCCCTGTTTTCAGGAAA‑CCTACGACGCTTTGGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTGAGGAGCATATCACGGt < 1:777067/223‑1 (MQ=255) atttttccttGGCATTGAGGTCAAATTTGATGTTGGGTTGGGCGCCAAAAAATAAGGGTTTTTCCACAAAGGG‑TAAAGGGTTGGGCC‑TTATATG‑CTCATCCCTGTTTGCAGAAAA‑CCTACGACGCTTTAGTTGCCAAAGTCGAGACATTAAAGGAAAAAGATCCAGAAAATTATAAGAAAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTGAGGAGCATATCACGGt < 1:545023/230‑1 (MQ=255) tGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGG‑TTAATGGTTGGGCG‑CTATATG‑CTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACACAGTTATTGGCGGTA‑GTCCC‑TAACATGATTgtggggaacataaagggcaa > 2:667045/1‑223 (MQ=255) tGTCGCTGGCAAGGACTAAAACATTGATGTTGAGATTGGCGCCAGCAAATAATGGATTTTCCACAAGGGG‑TTAATGGTTGGGCG‑TTATATG‑CTCCTCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTGAGGAGCATATCACGGTCaa < 2:115085/239‑1 (MQ=255) cTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGG‑TTAATGGTTGGGCG‑CTATATG‑CTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGATAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑TTCCC‑TAAAGTGATTGTGGATCacattccgtgcaaaccct > 2:216325/1‑225 (MQ=255) taaaCATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCAAAAGGGG‑TTAATGGTTGGGCG‑CTATATG‑CTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑Taa < 2:829879/193‑1 (MQ=255) cAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGG‑TTAATGGTTGGGCG‑CTATATG‑CTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCCGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GGCCA‑TAAAGAGAATTAGGGGCCTAACACGGGCAAACCATCATCACCgccct > 2:1006119/1‑234 (MQ=255) cAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGG‑TTAAGGGTTGGGCG‑CTATATG‑CTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCAAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCAt < 2:258093/238‑1 (MQ=255) atgatgaGATTGGCGACGACGAATAATGGATTTTCCACAAAGGG‑TTAATGGTTGGGCG‑CTATATG‑CTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAg > 2:524401/1‑166 (MQ=255) tgggaTTGGCGACGACGAATAATGGATTTCCCAAAAGGGG‑TTAAGGGTTGGGCG‑CTATATG‑CCCATCCCTGTTTCCAGGAAA‑CCTACGACGTTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGACCCTAAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCAT‑GGCa < 2:83978/235‑1 (MQ=255) gagtttggccgcgtggaATAGTGGATTTTCCCCAAGGGG‑TTAATGGTGGGTCG‑TTATATG‑CTCATCCCGGTTTGCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGGCGAGACATAAAAGGAAAAAGATCCTAAAAATTAACAGAGAAAGGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCAT‑GGCaa < 1:573393/223‑1 (MQ=255) gaggtgggcggcGCCAAATAATGGATTGTCCACAAGGGT‑TAAAGGGTGGGGGG‑TTAAAGGACTC‑TCCTGGTTTTCGTGAAT‑CTTCCGACTTTTTATTGGCGTAAGTCGAGACATTAAAGGGAAATGATCCAGAAAATTATCAGGAAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCAT‑GGCaa < 1:752415/232‑1 (MQ=255) tGGCGAAGAAGAATAAGTGATTCTCCAAAAAGTG‑TAAATGTTGGGGGT‑TTATATT‑CTCCTCCAGTTTTTCAGAAAT‑CCTACGCCGGTTGAGGTGCCAAAGCCGAGAATTTAAAGGGAAAAGTTCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCAT‑GGCAAGTCGtt < 2:58098/239‑1 (MQ=255) aataatGTATTTACCAAAAAGGG‑TTAAGGGTTGGGCG‑TTATATG‑CCCACCCCTGTTTTCAGAAAC‑CCGACGACGTTTTAGTTGCCGAAGTCGAAACATTAAAGGGAAAAGATCCTAAAAATTACCGGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCAT‑GGCAAGTCGTTAGGCTCTGGGa < 2:449470/239‑1 (MQ=255) aataatGGATTTTCCAAAAAGGG‑TTAATGGTTGGGCG‑TTATATG‑CTCATCCTTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTGAGGAGCATATCACGGt < 2:740106/189‑1 (MQ=255) atatgggATTTCCCACAAGGGG‑TTAAGGGTTGGGCG‑CTATATG‑CGCATCCCTGTTTCAAGGAAA‑CCTACGACGTTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCAT‑GGCAAGTCGTTAGGCTCTGGGa < 2:962341/233‑1 (MQ=255) ataatGGATTTCCCACAAAGGG‑TTAATGGTTGGGCC‑GTATGGG‑CTGCTCCCTGTTTTCAGGAAA‑CCTACGACGTTTTAGTTGCCGAAGTCAAGAAATAAAAGGGAAAAGACCCTAAAAATGATCAGAAAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCAT‑GGCAAGTCGTTAGGCTCTGGGa < 2:263335/238‑1 (MQ=255) tgtttttTCCCAAAGGGTTTAATGG‑TGGGCGTCATTATG‑CACTCCCCTGTTCTCAGAAAA‑CCTACCACGGTTTAGTGCCCGAAGACGAGACATAAAAGGGAAAAGATCCTAAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCAT‑GGCAAGTCGTTAGGCTCTGGGAAAAAt < 1:667045/236‑1 (MQ=255) aaaGGG‑TTAATGGTTGGGCG‑CTATATG‑CTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCC‑ATAAGAGATTTAGGAGACAATACCGGGCAATCCATACTCACCCGCATTCCGTGCT‑GGACAGTAGGTAAACTCTGGGAaaaaaaaaaaagtgacc > 1:378232/1‑230 (MQ=255) aaaGGG‑TTAATGGTTGGGCG‑CTATATG‑CTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGCATGTCCA‑‑AAAAAGATTTAGGGGGAAATCACGGTCAATACATCATTAACGGCATTCCGTCCTGGGCAAGT‑GGTAGGGACTGGGCaaaaaaaaaaacgagga > 1:979477/1‑230 (MQ=255) gg‑TTAATGGTTGGGCG‑CTATATG‑CTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTgag < 2:389250/154‑1 (MQ=255) ttAAGGGTTGGGGG‑CTATATG‑CTAATCCCTGTTTTAAGGAAA‑CCTTCGACGCTTTAGTTGCCGAAGTCGAGAAATTAAAGGAAAAAGATCCTGAAAATTATCAGAAAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCAT‑GGCAAGTCGTTAGGCTCTGGGAAAAATAAAGAC‑TGGTCACGGGTa < 2:516769/239‑1 (MQ=255) tAATGGTTGGGAG‑CTATATG‑CTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTGTATTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTAGCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCAT‑GGCAAGTCGTTAGGCTCTGGGAAAAATAAAGAC‑TGGTCACGGGTa < 2:163958/238‑1 (MQ=255) tAAGGGTGGGGCG‑CTAATTG‑CCCATCCCTGTTTCAAGGAAC‑CCTACGACGCTTTAGTTGCCGAAGTCGAGAATTTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCAT‑GGCAAGTCGTTAGGCTCTGGGAAAAATAAAGAC‑TGGTCACGGGTa < 2:949228/238‑1 (MQ=255) aaagggTTGGGCG‑CTATAGT‑CTCATCTCTGTTTGCAGGAAA‑CCTACGACGCTTTAGGTGCCGAAGTCGAGACTGAAAAGGGAAAAGATCCTGAAAATTTGCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCAT‑GGCAAGTCGTTAGGCTCTGGGAAAAATAAAGAC‑TGGTCACGGGTa < 2:385797/234‑1 (MQ=255) tGGGCG‑CTATATG‑CTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTG‑GTCCACAAAAGTG‑TTGAGGGGACAATACCGGGACATCCACCCTCCCCCGCATTCCCTAAG‑TGGAAGACGTTAGGCTCTTGGAAAAAAAAAAAC‑AGGACACGGGAaaaaaatgt > 1:167854/1‑234 (MQ=255) tGGGCG‑CTATATG‑CTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAATGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTTCGTCAT‑GGCAAGTCGTTTGGCTCTTGGGAAAATCAAGAC‑TGGTCACGGGGCAAAATTgg > 2:803006/1‑239 (MQ=255) gggtttgatG‑CCCACCCCTGTTTT‑AGGAAACCCTACGACGTTTTAGTTGCCGAAGACGAAAAATTAAAGGAAAAAGATCCTGAAAATTAAAAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCAT‑GGCAAGTCGTTAGGCTCTGGGAAAAATAAAGAC‑TGGTCACGGGTAAAATTTGGTg < 2:351388/231‑1 (MQ=255) ggcgtttttgtgtcccccgtGTTTTCAGAAAA‑CCTACTACGCTTTAGTGGACGAAGTCGAGAAAATAAAGGGAAAAAACTCTGAAAATTATGAGATAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCAT‑GGCAAGTCGTTAGGCTCTGGGAAAAATAAAGAC‑TGGTCACGGGTAAAATTTGGTg < 1:803006/220‑1 (MQ=255) gccttttttG‑CTCATTCTTTTTTTCAGAAAA‑CCTGCGATGTTTTAGTTGCAGAAAGCGAGACATTAAAGGGAAAAGATCCGGAAAATTATCAGAGAAAGGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCAT‑GGCAAGTCGTTAGGCTCTGGGAAAAATAAAGAC‑TGGTCACGGGTAAAATTTGGTg < 1:429919/230‑1 (MQ=255) ccgttataTT‑CTCATCCCTGTTTACAGGAAA‑CCTACGACGTTTTAGTTGCCGAAGTCGGGATATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAGGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCAT‑GGCAAGTCGTTAGGCTCTGGGAAAAATAAAGAC‑TGGTCACGGGTAAAATTTGGTg < 2:198836/234‑1 (MQ=255) tatG‑CTCATCCCTGTTTTCAGGAAA‑CCTACGACGTTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCAcc < 2:391027/164‑1 (MQ=255) aTCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTGAGGGGGCTATCACGGTCAATCCATCATCACCGGCATTCCGTCAT‑TGCAAAGCGTTAGGGCTTGGGGAAAATAAAAAC‑TGGCACCGGGGAAAAATTgtgggtgtccggtttg > 2:260639/1‑222 (MQ=255) tCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTGAGGAGCATATCACGGTCAATc < 1:688930/146‑1 (MQ=255) tCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAATGATTGAGGAGCATCTCCCGGTCAATc > 2:688930/1‑146 (MQ=255) tCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAAGGATTGAGGGGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCAT‑GGCAAGTCGTTAGGCTCTGGGAAAAATAAAAAA‑TGGTCACGGGGAAAAATTGGGGCTGGTGGTTATCg > 2:370340/1‑237 (MQ=255) ccTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTGAGGGGGCTATCACGGTCAATCCATCATCACCGGCCTTCCGTCAT‑GGCAAGTCGTTAGGGCCTGGGAAAAATAAAAACGGGGCCAGGGACAAAATTTGG‑GCTGGCGGTTTTCGtc > 2:548174/1‑237 (MQ=255) tGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTGCA‑TAAAAGGATTTAAGGACATATAACCGCACATACATCATCCACAGCAATCCGTCGT‑GGGAAGGAGGTCGGGTCGGGGGAAAATAAAAAT‑AGGTCACGGGGAAAAattttggcgggtggtatttttacgtt > 1:106103/1‑216 (MQ=255) tGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAAGGGTTTTGGAGCATCACCCGGGTAATCCCTCCTCAACGGCATTCCGTCCT‑GGGAAGTCGTTAAGCGTTGGGGGAAATAAAGAC‑AGGACACCGGGAAAATATTGTGTTGTTCggattagtccct > 1:565562/1‑227 (MQ=255) aGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTAt > 1:682489/1‑65 (MQ=255) aGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTAt < 2:682489/65‑1 (MQ=255) aGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GGCCA‑TAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCAT‑GGCAAGTCGTTAGGGTCTGGGGAAAATAAAAAC‑TTGGCACGGGGAAAAATTTGTGCTTGGCGTTTTCGGTCCTTCTTTCGt > 2:140652/1‑239 (MQ=255) aacctcagtagttttGGTGGCCGAATGGGAGAAATAAAAGGAAAAAGATCCTGAAAATAAACGGAAAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCAT‑GGCAAGTCGTTAGGCTCTGGGAAAAATAAAGAC‑TGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTtata < 1:140652/227‑1 (MQ=255) cgccgCTTGAGTTGCCGAAGTCGAGACATAAAAGGGAAAAGCTCCTAAAAATTACCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCAT‑GGCAAGTCGTTAGGCTCTGGGAAAAATAAAGAC‑TGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGaaa < 2:461046/236‑1 (MQ=255) tttAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAAGGGTTGTGGAGGATATAACGGTCAATCCATCCTCAACGGCATTCCGTACA‑GGCCGGTCGTTAGGCTCCTGGGAAAATAAAGAC‑TGGTGCCGGGGAAAATTTTGTGCGGGGTGGTATGGTCTCTTCTTTCTTTAAAAAGaaaaaaaaa > 1:11759/1‑235 (MQ=255) tttAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GGCAA‑TAAAATGATTTTGGGGGAAATAACGGTCAATCCACCATCACCGGCATTCCGTCAT‑GGCAAGTACTTAGGCTCCGGGGAAAAAAAAAAA‑TGGTCACGGGGCAAAATTGTGGCTGGTCGTTTTATGCTCTTTTTTTGTTTTAAGGaaaaaaaaa > 1:696866/1‑235 (MQ=255) taataagagacagaCATAAAAGGAAAAGGATCAAAAAAATTAACAAAAAAAAGCCGACACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTGAGGAGCATAGCACGGTCAATCCATCATCACCGGCATTCCGTCAT‑GGCAAGTCGTTAGGCTCTGGGAAAAATAAAGAC‑TGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGtca < 1:170519/228‑1 (MQ=255) tGCCGAAGTAGAGAAATTAAAGGGAAAAGATCCTAAAAATTATCAGAAAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCAT‑GGCAAGTCGTTAGGCTCTGGGAAAAATAAAGAC‑TGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGtca < 2:754814/239‑1 (MQ=255) cccGAAGGCGAGAAATTAAAGGGAAAAGTCCCGAAAAATTATGAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCAT‑GGCAAGTCGTTAGGCTCTGGGAAAAATAAAGAC‑TGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGtcat < 2:979477/238‑1 (MQ=255) ctaCAATAAGGGAAAAAGAGCTTGAAAATTAACAGAGAAAAGCCGGCCCAAAGTTATTGGCGGTA‑GCCCA‑AAAAGTTATTTAGGAACATATCACGGTCAAGCCATCATCACCGGCAGTCCGTCAT‑GGCAAGTCGTTAGGCTCTGGGAAAAATAAAGAC‑TGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGgga < 1:491648/237‑1 (MQ=255) aaCATTAAAGGGAAAAGATCCTAAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCAT‑GGCAAGTCGTTAGGCTCTGGGAAAAATAAAGAC‑TGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGgga < 2:11759/237‑1 (MQ=255) aGGGAAAAGATCCCTAAAATTAACAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCAT‑GGCAAGTCGTTAGGCTCTGGGAAAAATAAAGAC‑TGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAAc < 2:106103/239‑1 (MQ=255) aaaaGATCCTAAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGAGATTGAGGAGCATATCACGGTCAATCCATCATCCCCCGCATTCCGGCCT‑GGCAAGTCGTTAGGCTCTGGGGAAAATAAAGAC‑TTGTCACCGGGAAAATTTGGTGCTGGTCGTTATCGCCTCTTCTTTCGTTTTAAGGAAAAAAAGAAAGtcatccatttggg > 2:170519/1‑217 (MQ=255) tCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCAT‑GGCAAGTCGTTAGGCTCTGGGAAAAATAAAGAC‑TGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAaca < 2:696866/239‑1 (MQ=255) | TTTCTGGATGCAGATATCCAGAACAACCCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGATTGGCGACGACGAATAATGGATTTTCCACAAAGGG‑TTAATGGTTGGGCG‑CTATATG‑CTCATCCCTGTTTTCAGGAAA‑CCTACGACGCTTTAGTTGCCGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTA‑GTCCA‑TAAAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCAT‑GGCAAGTCGTTAGGCTCTGGGAAAAATAAAGAC‑TGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACA > NC_000913/3277213‑3277680 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |