| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 3,289,022 | C→A | 15.8% | Q71K (CAA→AAA) | yraJ → | putative outer membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,289,022 | 0 | C | A | 15.8% | 160.1 / 3.8 | 65 | Q71K (CAA→AAA) | yraJ | putative outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (39/15); new base A (2/9); total (41/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.29e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
AAAATATAAAAACACAGGTCATCAGGGAATGCCACAACGACACCACCAGGGACATAAACGCACACCGAAACAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAAATATGCAC‑AACTACCAGGAACTTATCAGGTTGA‑TATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAA‑TTACATTT‑ACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAATTTGATTTCA > NC_000913/3288784‑3289259 | aaaaTATAAAAAACCTGGGCCTCAGGGAATGCCACAACGACACCACCAGGGACATAAACGCACACCGAAACAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAAATATGcac < 1:492841/239‑1 (MQ=255) aaTATAAAAACACAGGTCATCAGGGAATGCCACAACGACACCACCAGGGACATAAACGCACACCGAAACAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGCATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑ACAAAAATATGCAC‑aa > 2:213802/1‑239 (MQ=255) aaaaaCACAGGTCATCAGGGAATGCCACAACGACACCACCAGGGACATAAACGCACACCGAAACAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAAATATGCAC‑AACTAcc < 2:620754/239‑1 (MQ=255) acacAGGTCCTCAGGGAATGCCACAACGACCCCACCAGGGACATTAACGCACCCCGAAACAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAAATATGCAC‑AACTACCAGGa < 1:54798/239‑1 (MQ=255) ggTCATCAGGGAATGCCACAACGACACCACCAGGGACATAAACGCACACCGAAACAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAACTATGCCC‑AACTACCAGGAACttt > 2:646387/1‑238 (MQ=255) caGGGAATGCCACAACGACACCACCAGGGACATAAACGCACACCGAAACAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCCCAAAAA‑GAGGAATGC‑AACAAACCGATCTTTCGCGTTTTTC‑AAAAAAATATGAAC‑AACTACCAGGAACTTATCAGGt > 2:72309/1‑239 (MQ=255) caGGGAATGCCACAACGACACCACCAGGGACATAAACGCACACCGAAACAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGAATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAATAATCGAC‑AACTACCAGAAACTTATCAGGt > 2:935230/1‑239 (MQ=255) aaTGCCACAACGACACCACCAGGGACATAAACGCACACCGAAACAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAAATATGCAA‑AACTACCAGGAACTTATCAGGTTGa‑ta > 2:264411/1‑239 (MQ=255) tGCCCCAACCAACCCCCCAGGGAAATAAACGCCAATCGAAACAGTTGGGGGTCAGCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGATTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAAATATGCAC‑AACTACCAGGAACTTATCAGGTTGA‑TATc < 1:213802/239‑1 (MQ=255) tGCCACAACGACACCACCAGGGACATAAACGCACACCGAAACAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGC‑AACCAACCGATCTGTCGCGTTTTTC‑AAAAAAATATGCAC‑AACTCCCAGGAACTTATCAGGTTGa‑ta > 2:778898/1‑237 (MQ=255) gCCACAACGACACCACCAGGGACATAAACGCACACCGAAACAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAAATATGCCC‑AACTa > 2:514764/1‑214 (MQ=255) cacaACGACACCACCAGGGACATAAACGCACACCGAAACAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAATAGTATTATTTCGACCCCATTATCCTGGAAACCACAAAAA‑GTGGGATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAAAAATGCAC‑AACTACCAGGAACTTTCCAGGttatatacggg > 1:797066/1‑230 (MQ=255) cacaACGACACCACCAGGGACATAAACGCACACCGAAACAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGAGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAAATAGGAAC‑AACTACAAGGAACTTATAAGGTTGT‑AATCTgc > 2:943402/1‑238 (MQ=255) ccacagaaacccccAGGGACATAAACGCACTCCGAAACAGTGGGCGCTCATCATTAAACGTTGTTTGCCGAAGGGGCTCACTGGCAGCGTCATGGTTTGCACTACCGCTAACGCCGAATAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAAATATGCAC‑AACTACCAGGAACTTATCAGGTTGA‑TATCTGGc < 1:684841/231‑1 (MQ=255) caaccccccccccaGGGACATAAACGCACCCCGAAACAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAAATATGCAC‑AACTACCAGGAACTTATCAGGTTGA‑TATCTGGCt < 1:943402/228‑1 (MQ=255) aaCGACACCACCAGGGACATAAACGCACACCGAAACAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAAATATGcac < 1:219005/204‑1 (MQ=255) aaCGACACCACCAGGGACATAAACGCACACCGAAACAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAAATATGcac > 2:219005/1‑204 (MQ=255) aaCGACACCACCAGGGACATAAACGCACACCGAAACAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAAATATGCAC‑AACTACCAgg > 2:907270/1‑214 (MQ=255) ccccaGGGACATAAACGCACACCGAAACAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAAATATGCAC‑AACTACCAGGAACTTATCAGGTTGA‑TATCTGGCTGAATaaaaa < 1:941619/237‑1 (MQ=255) gggACATAAACGCACACCGAAACAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAAATATGCAC‑AACTACCAGGAACTTATCAGGTTGA‑TATCTGGCTGATTAAAAAGAAgg > 2:362315/1‑239 (MQ=255) gACATAAACGCACACCGAAACAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑CAAAAAATATGCAC‑AACTACCAGGAAATTATAAAGGAAA‑AATCTAGCGGAAAAAAAAAAAGGtt > 1:429841/1‑239 (MQ=255) gACATAAACGCACACCGAAACAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGCGCTCACAGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATAATTTCGACCCCATTATGCTGGAAACCACAAAAA‑AGGGTATCA‑AAACAACCGCTCTGTCCCGTTTTCC‑ATAAAAAAAAGACA‑AACACCCGGGAACTTACCAGATTAA‑TACCTgcccaataagaaaaaaggg > 1:899336/1‑220 (MQ=255) cGAAACAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAAAAATGCAC‑AACTACCAGGAACTATTCAGGTTGC‑TATCTGGCTGAATAAAAAAAAGGTTTCAAAAAAAAAAA‑tt > 1:52819/1‑239 (MQ=255) ggCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAAATATGCAC‑AACTACCAGGAACTTATCAGGTTGA‑TATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAA‑TTACATTT‑Acc > 2:777798/1‑238 (MQ=255) ggCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GGGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAAATATGCAC‑AACTACCAGGAACTTATCAGGTTGT‑TATCTGGCTGAATAAAAAAAAGGGTTCACAGAAAAAAA‑TTAAAATT‑ACCg > 1:944467/1‑239 (MQ=255) catcatcaAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGCATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCG‑TTTTC‑AAAAAAATATGCAC‑AACTACCAGGAACTTATCAGGCTGA‑TATCTGGCTGAATAAAAAAAAGGTTTACCAGAAAAAAA‑TTAACTTT‑ACTGCCAATga > 2:365813/1‑238 (MQ=255) tGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAAATATGCAC‑AACTACCAGGAACTTATCAGGTTGA‑TATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAA‑TTACATTT‑ACCGCCAATGCAGAGCAACTTCTGCa > 2:337395/1‑238 (MQ=255) tGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAAATATGCAC‑AACTACCAGGAACTTATCAGGCTGA‑TATCCGGCTGAATAAAACGAAGGTTTCACAGAAAAAAA‑TTACATTT‑ACCGCAAATGCCGAGCAACTTTTGCa > 1:523858/1‑238 (MQ=255) gTGCTCACTGGCAGCGGCATTGTTTGCACTCCCGGAAACGCCGAAGGGTATTATTTCGACCCCATTATGCTGGAAACCAAAAAAA‑GTGGTATGA‑AAACAACGGATCTGTCGCGTTTTTC‑AAAAAAATATGCCA‑AACTACCAGGAATTTATCAGGTTGA‑TATCTGGCTGAATAAAAAGAAGGTTACAAAGAAAAAAA‑TTACATTT‑ACCGCCAATGCAGAGCAACTTCTGCAGCCACAGtt < 2:429841/239‑1 (MQ=255) tGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGCAGAGTATTATTTCGACCCCATTATGCTGGAAACCACCAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAAATATGCAC‑AACTACCAGGAACTTATCAGGTTGA‑TATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAA‑TTACATTT‑ACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAg > 2:97708/1‑239 (MQ=255) cAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAAATATGCAC‑AACTACCAGGAACTTATCAGGTTGA‑TATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAA‑TTACATTT‑ACCGCCCATGCAGATCAACTTCTGCAGCCCCAATTTACgggaaaaa > 1:962291/1‑233 (MQ=255) gCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAAATATGCAC‑AACTACCAGGAACTTATCAGGTTGA‑TATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAA‑TTACATTT‑ACCGCCAATGCAGAGAAACTTCTGCAGCCCAAGCTTACGGTAGAAAAACt > 2:338782/1‑238 (MQ=255) ttttttggccctcccGCTAAGCCCGAAGGGTTTTATTCCCCCCCCTTTTGTCTGAAAACCACAAAAA‑GTGGAATCC‑AACCAACCGATCTGCCGCGGTTTTA‑AAAAAAATATGCAA‑AACTCCCAGGAACTTATCAGGTTTA‑TATCTGGTTAAATAAAAAGAAGTTTGCAAAGAAAAAAA‑TTACATTT‑ACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTAc < 1:97708/225‑1 (MQ=255) ttGCACTACCTCTAACGACGAAAAGTATTATTTCGCCCCCATTATGCTGGAACCCAAAAAAA‑GTGGTATGC‑AAACAACCGTTCTGGCGCGTTTTTC‑AAAAAAAAATGCAC‑AACTACAAGGACTTTATCAGGTTGA‑TATCTGGCTGAAAAAAAAGAAGTTTTCACAGAAAAAAA‑TTACATTT‑ACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAg < 2:375817/238‑1 (MQ=255) tccACTCCCGCTAACGCAGAAGAGTTTTATTTCGCCCCAATTATGCTGAAACCCAAAAAAA‑GGGGTATGC‑AAACAACCGATCTGTCGGGTTTTTC‑AAAAAAATAGGCAC‑AACTACCAGGAACTTATCAGGTTGA‑TATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAA‑TTACATTT‑ACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCt < 2:48949/237‑1 (MQ=255) tGCCCTCCCGCAAACGCCAAAGAGTTTTATTCCGCCCCAATTAGGCTGAAACCCAAAAAAA‑GTGGAATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAGAATATGCAA‑ACCTACCAGAAACTTATCAGGTTGA‑TATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAA‑TTACATTT‑ACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCt < 2:155895/239‑1 (MQ=255) cTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAAATATGCAC‑AACTACCAGGAACTTATCAGGTTGA‑TATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAA‑TTACATTT‑ACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAg > 2:992353/1‑239 (MQ=255) cTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAAATATGCAC‑AACTACCAGGAACTTATCAGGTTGA‑TATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAA‑TTACATTT‑ACCGCCAATGCAGAGAAACTTCTGAAGCCACAGTTTACGGTAGAACAAATACGGCAGCTGGGATTTAAg > 2:44795/1‑239 (MQ=255) aaagagAAGGGTATTATTCCCACCCCATTATGCT‑GAAAACACAAAAAGGTGGTAGGC‑AAACAACCGATTTGTCGCGTTTTAA‑AAAAAAATAGGAAC‑AACTACAAGGAATTTAGCAGTTGGA‑TATTTGGCTGAAAAAAAAGAATGTTCAAAAAAAAAAAA‑TTACATTT‑ACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGt < 2:293802/233‑1 (MQ=255) gCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTA‑AAAAAAATATGCAC‑AACTACCAGGAACTTATCAGGTTGA‑TATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAA‑TTACATTT‑ACCGCCAATGCAGACCACCTTCTGCAGCCACAGTTTACGGTAAAAAAACAACGTGAACTGGGAATAAAGGGGGa > 1:490410/1‑239 (MQ=255) ttattTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAAATATGCAC‑AACTACCAGGAACTTATCAGGTTGA‑TATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAA‑TTACATTT‑ACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTCCGTGAGCTGGGAATTAAGGTGGTTGAAATCCCgg > 2:370585/1‑239 (MQ=255) agtcgcaCCCAATTACGCTGAAAACCAAAAAAA‑GGGGTATGA‑AAACAACCGTTCTGGCGGGTTTTTA‑AAAAAAATAGGCAA‑ACCTACCAGGAACTTATCAGGTTGA‑TATCTGGCTAAATAAAAAGAAGGTTT‑ACAAAAAAAAATTTACTTTT‑ACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGgcg < 2:797066/233‑1 (MQ=255) tCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAAATATGCAC‑AACTACCAGGAACTTATCAGGTTGA‑TATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAA‑TTACATTT‑ACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGACAAACAACGTGAGCTGGGTATTAAGGGGGATGAAAACCCGGCgctg > 1:467896/1‑239 (MQ=255) cGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAAATATGCAC‑AACTACCAGGAACTTATCAGGTTGA‑TATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAA‑TTACATTT‑ACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAAAAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCgctg > 2:418738/1‑238 (MQ=255) aCCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTTTCGCGTTTTTC‑AAAAAAATATGCAC‑AACTACCAGGAACTTATCAGGTTGA‑TATCTGGCTGAATAAAAAGAAGGTTTAACAGAAAAAAA‑TTACATTT‑ACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGTTCAAACAAACACGTGAGCTGGGGCTTAAGGCGGATGAAATccctgtcccttaa > 1:293802/1‑229 (MQ=255) aCCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAAATATGCAC‑AACTACCAGGAACTTATCAGGTTGA‑TATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAA‑TTACATTT‑ACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCgctg > 2:713532/1‑236 (MQ=255) aCCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAAATATGCAC‑AACTACCAGGAACTTATCAGGTTGA‑TATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAA‑TTACATTT‑ACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCcctggct > 2:646135/1‑239 (MQ=255) aCCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAAATATGCAC‑AACTACCAGGAACTTATCAGGTTGA‑TATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAA‑TTACATTT‑ACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGAGAGCTGGGGATTAAGGGGGAGGAAAACCGGGCgcgggcg > 1:997828/1‑238 (MQ=255) aCCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAAATATGCAC‑AACTACCAGGAACTTATCAGGTTGA‑TATCTGGCTGAATAAAAAGAAGGTTTAACAGAAAAAAA‑TTACATTT‑ACCGCCAATGCAGAGCAACTTCTGCAGCCCCAGTTTACGGTGGAACAACTACGTGAGTTGGGTATTAAGGGGGAGGAAAACCCgagacgggcg > 1:682070/1‑230 (MQ=255) ccATTATGCTGGAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAAATATGCAC‑AACTACCAGGAACTTATCAGGTTGA‑TATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAA‑TTACATTT‑ACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGACTTAAGGGGGATGAAATCCCGGGGCTGGCTGaa > 1:509067/1‑239 (MQ=255) cATTATGCTGGAAACCAAAAAAA‑GTGTTATGC‑AAACAACCGATCGGTCGCTTTTTTA‑AAAAAAATATGCAC‑AACTACCAGGAATTTACAAGGTTGA‑TATCTGGCTGAAAAAAAAGAAGGTTTCACAAAAAAAAA‑TTACATTT‑ACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGaaa < 2:509067/239‑1 (MQ=255) ttgcggAAACCCAAAAAA‑GGGGGAAGGAAAACAACCGATCGTTCGGTTTTTTC‑AAAAAAATATGACA‑AACTCCCAGGAACTTATCAGGTTGA‑TATCTGGCTGAATAAAAAGAAGTTTTCAAAGAAAAAAA‑TTACATTT‑ACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGa < 2:52819/235‑1 (MQ=255) ggAAACCAAAAAAA‑GGGGAAACA‑AAAAAACCGATCTGTCGCTTTTTTA‑AAAAAAAATGGCAA‑AACTACAAGAAACTTAAAGGGTTGA‑TATCTGGCTGAATAAAAAGAAGGTTTCAAAAAAAAAAA‑TTAAATTT‑ACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACg < 2:944467/239‑1 (MQ=255) gAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAAATATGCAC‑AACTACCAGGAACTTATCAGGTTGA‑TATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAA‑TTACATTT‑ACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGGAATCCCGGCGCTGGCTGAAAAAGATGACGa > 2:72452/1‑239 (MQ=255) gAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAAATATGCAC‑AACTACCAGGAACTTATCAGGTTGA‑TATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAA‑TTACATTT‑ACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGa > 2:758648/1‑239 (MQ=255) cacaAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAAATATGCAC‑AACTACCAGGAACTTATCAGGTTGA‑TATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAA‑TTACATTT‑ACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGAATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCg > 2:627871/1‑239 (MQ=255) aaaaat‑GTGGTAGGA‑AAACAACCGATTTGTCGCTTTTTTC‑AAAAAAATATGCCC‑AACTACCAGGAACTTATCAGGTGTA‑TATCTGGATAAAAAAAAAAAAGGTTTCAAAGAAAAAAA‑TTACTTTT‑ACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTg < 2:523858/238‑1 (MQ=255) a‑GTTGTATGC‑AAACCCCCTGTCTTGCGCTTTTTTA‑AAAAAAAGAGTCCA‑AACCACCAGCAAATTAACAGGTTAA‑TTTCTGGCTAAATAAAAAGAAGGTTTCAAAGAAAAAAT‑TTACATTG‑ACCGCCAATGCAGAGCAGCTTCTGCAGCCACAGGTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCaa < 1:264411/239‑1 (MQ=255) ggTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAAATATGCAC‑AACTACCAGGAACTTATCAGGTTGA‑TATCTGGCTGAATAAAAAGAAGGTTTca > 1:470717/1‑99 (MQ=255) ggTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAAATATGCAC‑AACTACCAGGAACTTATCAGGTTGA‑TATCTGGCTGAATAAAAAGAAGGTTTca < 2:470717/99‑1 (MQ=255) aaaCAACCGATCTGTCGCGTTTTTC‑AAAAAAATATGCAC‑AACTACCAGGAACTTATCAGGTTGA‑TATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAA‑TTACATTT‑ACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCCCTTGac > 2:399379/1‑238 (MQ=255) aacaccCGATCTGCCGCGTTTTAC‑AAAAAAATATGCAC‑AACTACCAGAAACTTATCAGGTTGA‑TATCTGGCTAAATAAAAAGAAGGTTTCAAAAAAAAAAA‑TTACATTT‑ACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAAc < 2:467896/239‑1 (MQ=255) caccgtatctgccctttttCAAAAAAAATACGCCA‑ACCTCCAAGGAACTTATATGGTGTA‑TATTTGGCTGAAAAAAAAAAAGTTTCCACAAAAAAAAA‑TTACTTTT‑ACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACaaa < 2:609345/225‑1 (MQ=255) gtttttttgctttttt‑AAAAAAATTAATACAAACTTCCAGAAAGTTAACAGGTTGT‑TATGTGGCTAAAAAAAAAGAAGCTTCAACAGAAAATAA‑TTACTTTT‑CCCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCAt < 1:362950/229‑1 (MQ=255) aTCTGTCGCGTTTTTC‑AAAAAAATATGCAC‑AACTACCAGGAACTTATCAGGTTGA‑TATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAA‑TTACATTT‑ACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAAt > 2:459428/1‑181 (MQ=255) aTCTGTCGCGTTTTTC‑AAAAAAATATGCAC‑AACTACCAGGAACTTATCAGGTTGA‑TATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAA‑TTACATTT‑ACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCAt > 2:274088/1‑238 (MQ=255) tGTCGCGTTTTTA‑AAAAAAATGGGCAA‑AACTTCCAGGACCTTATCAGGTTGA‑TTTTAGGCTGAAAAAAAGGAAGGTTTCAAAAAAAAAAA‑TTACATTA‑ACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTccc < 2:899336/239‑1 (MQ=255) tataaaaaacaaaaaaTACGAAC‑AA‑TACCCAGAACTTTACTAGTTTATTATTTGGTTAAAAAAAAAAAAGG‑TTCACAAAAAAAAA‑TTTCCTTTAACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTa < 1:928614/229‑1 (MQ=255) cac‑aACTACCAGGAACTTATCAGGTTGA‑TATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAA‑TTACATTT‑ACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATT‑CCGGTACAGCTGCTGAATTTGATTTCa > 2:248469/1‑239 (MQ=255) | AAAATATAAAAACACAGGTCATCAGGGAATGCCACAACGACACCACCAGGGACATAAACGCACACCGAAACAGTTGGCGCTCATCATCAAACGTTGTTTGCCGATGGTGCTCACTGGCAGCGGCATGCTTTGCACTACCGCTAACGCCGAAGAGTATTATTTCGACCCCATTATGCTGGAAACCACAAAAA‑GTGGTATGC‑AAACAACCGATCTGTCGCGTTTTTC‑AAAAAAATATGCAC‑AACTACCAGGAACTTATCAGGTTGA‑TATCTGGCTGAATAAAAAGAAGGTTTCACAGAAAAAAA‑TTACATTT‑ACCGCCAATGCAGAGCAACTTCTGCAGCCACAGTTTACGGTAGAACAACTACGTGAGCTGGGTATTAAGGTGGATGAAATCCCGGCGCTGGCTGAAAAAGATGACGATAGCGTGATCAACTCGCTTGAACAAATCATTCCCGGTACAGCTGCTGAATTTGATTTCA > NC_000913/3288784‑3289259 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |