Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	3,507,166	T→C	13.9%	S57G (AGC→GGC)	yhfT ←	inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,507,166	0	T	C	13.9%	190.4 / 4.2	96	S57G (AGC→GGC)	yhfT	inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (43/37);  new base C (5/10);  total (48/48)
Fisher's exact test for biased strand distribution p-value = 1.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01

GGCGAGCTTAATTCCCCCAGGCTACCTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAG‑AAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTC  >  NC_000913/3506928‑3507393                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                     ggCGAGCTTAATTCCCCCAGGCTACCTAATACAGCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCt                                                                                                                                                                                                                                      <  2:324565/239‑1 (MQ=255)   cGAGCTTAATTCCCCCAGGCTACCTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGA‑AAAAGAGAACCCCACCGAGGCCACAAAA‑ACAAAGCCCa                                                                                                                                                                                                                                    >  1:620437/1‑239 (MQ=255)       cTTAATTCCCCCAGGCTACCTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGCAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCCCCGAGGCCACAAAA‑CCAATGCTCAGAcc                                                                                                                                                                                                                                >  2:479931/1‑239 (MQ=255)        ttAATTCCCCCAGGGCACCTAATACACCCACCGGCAGCGCGGCCAGCAGCGGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGAcc                                                                                                                                                                                                                                <  1:593758/238‑1 (MQ=255)                cccAGGCTACCTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGCGGCCACAAAA‑CCAATGCTCCGACCAAAAGCGAt                                                                                                                                                                                                                       >  2:39311/1‑239 (MQ=255)                    ggCTACCTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATACTCAGACCaaaaagcataac                                                                                                                                                                                                                    >  2:340628/1‑230 (MQ=255)                       tACCTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTccc                                                                                                                                                                                                                 >  2:703844/1‑238 (MQ=255)                       tACCTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATCCGTTGAGCAGCCCGGTTTTCAGGGG‑AAAAGAGATCCCCCCCGAGGCCACCAAA‑CCAATGATCAAACCaaaaaggaaaaaccc                                                                                                                                                                                                                 >  1:366094/1‑227 (MQ=255)                         ccTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCCCAGACCAAAAGCGATACTCCCCg                                                                                                                                                                                                               >  2:314130/1‑238 (MQ=255)                         ccTAATACATCCACAGGCAGCGCGGTCAGCAGCTAGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGACGACAGAGTAACCATGCGTTGAGCGTCCCGGCATTCAGGGA‑AAAAGAGATCCCCACCCAGGCCACAAAC‑ACACAACTAAGAACAAAAGcagaaacaccccc                                                                                                                                                                                                              >  1:20243/1‑227 (MQ=255)                              tACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AACAGAGATCCCCACCGAGGCCCCAAAA‑CCACTGCTCAGCCCAAAAACGATAATCcccgccccgc                                                                                                                                                                                                         >  2:57528/1‑239 (MQ=255)                               accaccACCGGCAGCGGGGTCGCGCGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGGGGATATCGGTAGGAAGAAAGAGTAACCAGGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTcgcg                                                                                                                                                                                                        <  1:39311/235‑1 (MQ=255)                               aCATCCACCGGCAGGGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTcgcg                                                                                                                                                                                                        <  2:366094/239‑1 (MQ=255)                                ggcgcaggtgtgtgaaaagagacagcTTGTTTACCGGAAACAGGCAAGTAAGGGTCAACCCGGCCCAGAGAGCGCCAAGACCAAACGCCATCAGGCTTTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCAGGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATCCTCCCCGCCTcgcg                                                                                                                                                                                                        <  1:635740/216‑1 (MQ=255)                                cATCCCCCGGACGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACCCGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGa                                                                                                                                                                                                       <  2:794267/239‑1 (MQ=255)                                 aTCCACCGGCAGCGCGGTCAGGCGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGAc                                                                                                                                                                                                      <  2:952856/239‑1 (MQ=255)                                   ccACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCCCAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCCCCCGCCgg                                                                                                                                                                                                    >  2:782624/1‑239 (MQ=255)                                   ccACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAgatgtctcttatacacacccaac                                                                                                                                                                                                     >  2:910101/1‑216 (MQ=255)                                   ccACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTccc                                                                                                                                                                                                                 <  2:889034/226‑1 (MQ=255)                                   ccACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACgg                                                                                                                                                                                                    >  2:836776/1‑239 (MQ=255)                                   ccACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACgg                                                                                                                                                                                                    <  2:799757/239‑1 (MQ=255)                                   ccACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACgg                                                                                                                                                                                                    >  2:6836/1‑239 (MQ=255)                                   ccACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGAAACTCCCCGCCTCGCGACgg                                                                                                                                                                                                    >  2:75570/1‑239 (MQ=255)                                   ccACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGAAACTCCCCGCCTCGCGACgg                                                                                                                                                                                                    >  2:52880/1‑239 (MQ=255)                                   ccACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTAAGACCAAAAGCGCAACCACCCGCCTCGCGCCgg                                                                                                                                                                                                    >  2:159926/1‑239 (MQ=255)                                   ccACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCCCAGACCAAAAGCGATACTccc                                                                                                                                                                                                                 >  1:889034/1‑226 (MQ=255)                                   ccACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGGTTTCAGGGC‑AAAAGAGATCCCCACCGAGGCCACCAAA‑CCAATGCTCAGACAAAAAGAGATACTCCCCCCCTcccgcggg                                                                                                                                                                                                    >  2:606045/1‑235 (MQ=255)                                   ccACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGAAGCCGAGGAAATCGGAAGGAAGAAAGCGTAACCATGCGTTGCCAAGCCCGGGTTTCAGGGA‑AAAAAGTATCCCCCCCGAGGCCACCAAA‑CCAATGCCAAGACCAAAAACGATACACCCCGCCACGCGAAgg                                                                                                                                                                                                    >  1:843513/1‑239 (MQ=255)                                    ccccGGCACGGCGGTAAACATCTGGTTTCCTGGCAACAGGCAAGTAAGGATCAACCCGCCCCAGATAGCGCAAAGACCAAACGCTATCAGGCTGTTTACCGCAAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGt                                                                                                                                                                                                   <  1:479931/237‑1 (MQ=255)                                    cACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGAAAGGATCAACACGCCCCAGATAGCTCAAAGAACAAACGCCATCAGGCTGTTTCTCACCAGGACGCCCAGGATATCGGTAGGAGGAAAGAGTAACCTTGCGTTGAGAACACCGGTTTTCCGGGA‑CAAAAGGATGCCCACCCAGGCCACAAAA‑CCAATGATCAGACCCAAAAGGAAACTCCCCGCCCcgcgcaggg                                                                                                                                                                                                   >  1:926591/1‑234 (MQ=255)                                     aCCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCAAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCCGGACGCCGCGGATATCGGTAGGAAGAAAGAGGAACACTGCGTTTACAAGCCCGGTTTTAAGGGTCAAAAGA‑ATCCCCACCGGGGCACCAAAA‑CCACAGCCCAAACCAAAAAGGATAACCCACCCCCCGTGACGGtt                                                                                                                                                                                                  >  1:498667/1‑239 (MQ=255)                                             cgcgGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAg                                                                                                                                                                                           >  2:645934/1‑238 (MQ=255)                                             cgcgGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCAAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGGCCAGGCTCATATAg                                                                                                                                                                                           >  2:979584/1‑238 (MQ=255)                                             cgcgGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCAGAGGACATCGGAAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑ACAAGAAATCCACCCCGAGGCCCCAAAA‑CAAAAGCTCAGCCCAAAACCAAAACTCCCCGCCACGCGCCGGGTCaaaaag                                                                                                                                                                                           >  1:126762/1‑233 (MQ=255)                                                             ttttagtgCAACGGGCAAGTAAGGCACAACCCCCCCCAGAAGGCCCCAAGCCCAAACGCCATCAGGCTTTTTAGCGCCAGGACGCCGGGGATATCGGTAGGAAGAAAGGTTAACCAGGCGTTGACGAGCCCGGTTTTCAGGGA‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAAGGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCgg                                                                                                                                                                          <  1:683862/232‑1 (MQ=255)                                                                   ggCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCata                                                                                                                                                                                              >  2:635740/1‑213 (MQ=255)                                                                      aaCAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGCCGGTTCCTATAACCTTCAATCAGTTGCGGCAGGCTCgg                                                                                                                                                                 >  2:623013/1‑239 (MQ=255)                                                                      aaCAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAACGGTAACCCCCCCCCCCGAACGGTTCAAATAGCCCTCAAAAAGTTGGGGCAGGagggg                                                                                                                                                                 >  1:319676/1‑235 (MQ=255)                                                                       aCAGGGTAGTACGGTTCAACCCGCCCACGAAAGCGCCAAGACCAAACGCCATACGGCTTTTTATCGCCAGGACCCCGAGGATATGGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCggg                                                                                                                                                                <  1:979584/239‑1 (MQ=255)                                                                                  aGGATCCACCCGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTAGCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCg                                                                                                                                                       <  2:532871/237‑1 (MQ=255)                                                                                             gCCCCAGATAGCCCCAAGACCAAACGCAATCAGGCTGTTTAACGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCCCCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAg                                                                                                                                          <  2:620437/239‑1 (MQ=255)                                                                                                         gcCAAGACCAAACGCCATCAGGCTTTTTATCGCCAGGACGCCGAGGATAGCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGa                                                                                                                               <  2:26202/238‑1 (MQ=255)                                                                                                             aGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCCTGAAAAACAGccgccggcggaggcg                                                                                                                           >  2:721016/1‑229 (MQ=255)                                                                                                             aGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATgcg                                                                                                                           >  2:744545/1‑238 (MQ=255)                                                                                                                   aaCGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATTCCGGCCTGAAAAAAAGCCGCCGAGCGATGGGCCAG‑aac                                                                                                                   >  2:846981/1‑238 (MQ=255)                                                                                                                   aaCGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGGTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGTAGCCGCCCTGAAAAAAATCCCCCGAGCGATGCGCCacacg                                                                                                                    >  2:335711/1‑235 (MQ=255)                                                                                                                           taggcgttttACCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGACCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAG‑AAGCGATGTCa                                                                                                           <  2:375110/232‑1 (MQ=255)                                                                                                                               ctttttAGCGCCAGGCCCCCGAGGATTTCGGTGGAAAGAAAGTGGCACCAGTCGTTGAGCACCCCGGTTTTCAGGGT‑AAAAGAGATCCCCCCCGAGGCCAGAAAT‑CCAATGCTCAGACCAAAAGCGAGACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAG‑AAGCGATGTCATa                                                                                                         <  1:124233/234‑1 (MQ=255)                                                                                                                                 ttttATCCCCAGGCCCCCGAGGTAATCGTGAGGAAAAAAAGTTAACCATGCGGTGAGCCCCCAGGTTTTCAGGGA‑AAAAGAAACCCCCACCGGGGCCACAAAA‑CCAATGCCCAGACCAAAAGCGATCCTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAG‑AAGCGATGTCATACCCg                                                                                                     <  1:112219/238‑1 (MQ=255)                                                                                                                                  tttATCGCCAGGACGCCGCGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCCATGCTCAGAACAAAAGCGATACTCCCCGCCCCGCGACGGTTCATATAGCCTTCAATCAGTTGAGGCAGGACAGGGCGGAGGCCGTCATGAAAAAAGACCCCCCAGGCATGCGCCAG‑AAGccgggacacacaccg                                                                                                    >  1:808673/1‑225 (MQ=255)                                                                                                                                  tttATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGGTGCGCCAG‑AAGCGATGTCATACCCg                                                                                                     >  2:112219/1‑238 (MQ=255)                                                                                                                                        cgcagcaagCCGGGGATCTCGGGAGGAAAAAAGGTTAACAGTGGTTTGAGCCGCCGGGTTTCCAGGAG‑AAAAGAGTCCCCCCCCGGGGCCCCAACC‑CCAATGCTCCGACCAAAAGCGATGCTCCCCGCGTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAG‑AAGCGATGTCATACCCGTCAGGCa                                                                                              <  1:335711/231‑1 (MQ=255)                                                                                                                                         ccAGGGCGCCGGGGGTATCGGTAGGAAGAAAGAGTAACCAGGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCCCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAG‑AAGCGATGTCATACCCGTCAGGCAc                                                                                             <  2:865265/239‑1 (MQ=255)                                                                                                                                            ggACGCCGAGGATATCGGTAGGACGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTAATATAGCCTTCAATAAGTTGCGGCAGGAGCCGGCGGGTGCCCGCCTGAAAAACAGCCGCCCGGCGAAGGCCCCG‑AAACGCGGGCATAACCGTTAggacagc                                                                                           >  1:797752/1‑233 (MQ=255)                                                                                                                                            ggACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCCGGATCGGGCGGATGCCGTCATGAAAAACAGCCTCCGAGCGATGCCCCCG‑AAGCGATGTCATACCCGTCAGGAACGca                                                                                          >  2:103819/1‑238 (MQ=255)                                                                                                                                            ggACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCCTGAAAAACAGCCGCCGAGCGATGCGCCCG‑AAGCGATGGCATACCCGCCAGGCACGc                                                                                           >  2:887047/1‑238 (MQ=255)                                                                                                                                              aCGCCGAGGATTTCGGTCGGAAGAAAGAGTAACCAGGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAG‑AAGCGATGTCATACCCGTCAGGCACGcca                                                                                         <  2:319676/238‑1 (MQ=255)                                                                                                                                                       ttatCGGGAGGAAAAAAGAGAAACCAGGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGACCCCCACCGAGTCCAAAAAC‑CCAATGCTCAGACCAAAAGCGAGACCCCCCGCCTCGGGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAG‑AAGCGATGTCATACCCGTCAGGCACGCCACCACGATaa                                                                                <  1:782624/237‑1 (MQ=255)                                                                                                                                                       atttgatggggAAGAAAGGTTACCCCGGCGTTGAGCACCGCGTTTTTGCGGGT‑AAAAGAGCACCCCACCGAGGCCACAAAC‑ACAATGCCCAAACCAAAAGCGATAGTCCCCGCCGCGCGACGGTTCATATAGCCTGCAATCAGTTGCGGCAGGGTCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAG‑AAGCGATGTCATACCCGTCAGGCACGCCACCACGATaa                                                                                <  1:70626/229‑1 (MQ=255)                                                                                                                                                        tctCGGTAGGAAGAAAAAGGCACCAGGGGTGGAACACCCCGGTTTTAAGGGA‑AAAAGGGGCCCCCCCCGAGGCCCAAAAC‑CCAATGCTACGACCAAAAGCGATACGCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAG‑AAGCGATGTCATACCCGTCAGGCACGCCACCACGATAAt                                                                               <  1:52880/236‑1 (MQ=255)                                                                                                                                                           cGGTAGGAAGACAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAGGCGATACTCCCCGCCTcgcg                                                                                                                                                                                                        >  1:848330/1‑115 (MQ=255)                                                                                                                                                           cGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTcgcg                                                                                                                                                                                                        <  2:848330/115‑1 (MQ=255)                                                                                                                                                            ggTAGGAAGAAAGAGTAACCACGCGTTACGCAGCCCGTTTTTCAGGGT‑AAAAGAGAACCCCACCGAGGCCAAAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAG‑AAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGaa                                                                          <  2:498667/239‑1 (MQ=255)                                                                                                                                                               aGGAAAAAAAGGTAACCAGGGGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGGGATCCCAACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAG‑AAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAata                                                                        <  2:991918/238‑1 (MQ=255)                                                                                                                                                                 gaaaaaaaggtcaCCATGCGTTGAACAGCCCGGTTTTCAGGGT‑AAAAGAAACCCCCACCGAGGCCAAAAAA‑CCAATGCTCAAACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAG‑AAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAAtata                                                                      <  2:843513/226‑1 (MQ=255)                                                                                                                                                                 agagaaagaGTCACCATGCTTTGAGCAGCCGGGTTTTCAGGGT‑AAAAGAAATCCCCACCGGGGCCACAAAA‑CCAATGCTCAGCCCAAAAGCGATACTCCCCGCCTCGCGACGGTCCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAG‑AAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAAtata                                                                      <  2:756915/236‑1 (MQ=255)                                                                                                                                                                 aaaaaaaaacaaaCCAGGCGTGTCGCACCCCGGTTGTCAGGGA‑AAAAGGAGCCCCCACCGAGGCCAAAAAC‑CCAAGTCTCAGACCAAAAGCGATACTCACCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAG‑AAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAAtata                                                                      <  1:845132/227‑1 (MQ=255)                                                                                                                                                                  aagaaagaGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGTTCCGGCGGATGCCGTCCTGAAAAACAGCCGCCGAGCGATGCGCCAG‑AAGCGATTGCATACCCCTCAGGCACCCCCCCACGAAAAACTAAAaataaa                                                                    >  1:522424/1‑237 (MQ=255)                                                                                                                                                                  aagaaagaGGAACCAGGCGTTGAGCACCCCGGTTTTAAGGGT‑AAAAGAGACCCCCACCGACGCCAAAAAA‑CCAATGCTCAGACCAAAAGCTATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAG‑AAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATAc                                                                     <  2:757121/238‑1 (MQ=255)                                                                                                                                                                  aagaaaagGTAACCATGGGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCCCCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGGGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAG‑AAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATAc                                                                     <  2:522424/238‑1 (MQ=255)                                                                                                                                                                  aaaaaaggtacaCCATGCGTTGACCACCCTGGTTTTCAGGGA‑AAAAGAGATCCCCCCCGAGG‑CACAAAACCCAATGCCCAGCCCAAAAGCGATCCTCCCCGCCCTGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAG‑AAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATAc                                                                     <  1:836776/227‑1 (MQ=255)                                                                                                                                                                   agaaaggGTAACCATGGCTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAAATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAG‑AAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAAtata                                                                      <  2:725492/236‑1 (MQ=255)                                                                                                                                                                   aaaaagaGTAACCAGGCGTTGAGCAGCCGGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATTCCCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAG‑AAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAg                                                                   <  2:126762/237‑1 (MQ=255)                                                                                                                                                                            aaCCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGACTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGAACGGGCGGATGCCGTCCTGAAAACCAGCCGCCGAGCGATGCGCCAG‑GAGGGGAGTCATAACCGTCCGGACCCCCACCCCGATaacccaaacaaacagaacccaaa                                                           >  1:42266/1‑217 (MQ=255)                                                                                                                                                                             aCCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCTGGCGGATGCCGTCATGAAAAACAGGCGCCCGGCGATGCGCCCG‑AAGCGATGTCATACCCGCCCGGCAACCCCCCACCCAAATCTGAATATACacaccccaaacc                                                         >  1:703296/1‑228 (MQ=255)                                                                                                                                                                                   cGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGt                                                                                                                                                                               <  2:583859/116‑1 (MQ=255)                                                                                                                                                                                   cGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGt                                                                                                                                                                               >  1:583859/1‑116 (MQ=255)                                                                                                                                                                                   cGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAG‑AAGCGCTGTCATACCCGTCAGGCACGCCACCACGATAATCTGATTATACAGTTCCATAATCGGGc                                                     >  2:346717/1‑237 (MQ=255)                                                                                                                                                                                        agcagcCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATTACAAACAGCCGACGAGCGATGCGCCGG‑AACCGATTGCATACCCGTTAGGCACCCCCCCAACATAACCTGAAAATAAATAACCAACGTCGTGCCtttttt                                              >  1:109585/1‑237 (MQ=255)                                                                                                                                                                                        agcagcCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGGTGCGCCAG‑AACCGATGTCATACCCGTCAAGCACGCCCCCCCGAAAATCCTAATATAAAGATCCATAGCCCGGCCtttttt                                              >  1:833196/1‑237 (MQ=255)                                                                                                                                                                                         acagcCCGTTTTTCAGGGT‑AAAAGATACCCCCCCCGAGGCCAAAAAA‑CCAATGCCCAAACCAAAGGGAATACCCCCCGCCTCGCGACGGTTCAAATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAG‑AAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTAt                                              <  1:703844/237‑1 (MQ=255)                                                                                                                                                                                            gcCCGTTTTTAAGGGT‑AAAAGGGCTCCCCCCCGAGGCCCCAAAA‑CCAATGCCCAGCCCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAG‑AAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCa                                          <  2:402500/239‑1 (MQ=255)                                                                                                                                                                                            ccctgtttttggggat‑AAAAGAGCTCCCCCCAGGGCCCAAAAAA‑CAAAGGCTCAGCCCAAAAGTAGGCCCCCCCGCCGGGCGAAGGTTCAAATGACCTTCAATCAGGTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAG‑AAGCGATGTCATACCCGTCAGGCACGCCACCACGATAAGCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCa                                          <  1:741393/224‑1 (MQ=255)                                                                                                                                                                                            ccccGTTTTTCAGGGT‑AAAAGAGATCCCCCCCGAGGCCAAAAAA‑CCAAAGCTCAGACCAAAAGCGATACCCCCCCGCGCGCGACGGTTCATATAGCCTTCAATCAGTGGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAG‑AAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCa                                          <  1:744545/238‑1 (MQ=255)                                                                                                                                                                                             cccTGTTATCAGGAG‑AAAACAGTTCCCCACCGGGGCCACAAAA‑CCAATGCTCAACCCAAACGCGATTCTCCCCGCCTCGCGACGGTACATAAAGCCTTCAATCAGTTGCGGCAGGAGCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAG‑AAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAg                                         <  1:340628/239‑1 (MQ=255)                                                                                                                                                                                              ccGGTTTGCAGGGT‑AAAAGTGATCCCCACCGAGTCCACAAAA‑CCAATGCCCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAG‑AAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGa                                        <  2:808673/239‑1 (MQ=255)                                                                                                                                                                                              ccGGTTTGCAGGGA‑AAAAGATAGCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAG‑AAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGa                                        <  2:43432/239‑1 (MQ=255)                                                                                                                                                                                                  ttttCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACCCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAAc                                                                                                                                            <  2:862258/136‑1 (MQ=255)                                                                                                                                                                                                        aggtaaaaaGGGATCCCCCGCGAGCTCACAAAA‑CCAAGGCACAGACCAAAGGCGATACTCCCCGTCTCGGGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAG‑AAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATc                               <  1:75570/234‑1 (MQ=255)                                                                                                                                                                                                                  agaCCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAG‑AAGCGATGTCATACCCGTCAGGCAc                                                                                             <  2:383366/167‑1 (MQ=255)                                                                                                                                                                                                                   aacccccACCGAGCCCACAAAA‑CCAAGGGCAAGACCAAAAGGGATTCTCCCCGCCTCGCGACGGTTCATATAGCCTGCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAG‑AAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGa                      <  2:42266/234‑1 (MQ=255)                                                                                                                                                                                                                    aTCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGCGCGATGCGCCAG‑AAGCGATGTAATACCCGTCAGGCACGCCCCCACGATAATCTGAAAATACAGAACCATAGTCAGGCCTTTTTTTTCACTTGGTTAAACAGCACCGGaaac                   >  1:291637/1‑237 (MQ=255)                                                                                                                                                                                                                    aTCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACAAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGACAGGCTCGGGCGGATGCCGTCATGAAAAACAGCCGGCGACCGATGCGCCAG‑CAGCGATGTCAAACCCAGCCGGCACGCCCACACGAAAATCTGGATATACAGATCCATAGCCATGCCTTTTATTTAAGATGGATAATCAgaagagggaaa                   >  1:665041/1‑229 (MQ=255)                                                                                                                                                                                                                      ccccACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGGGCGCGGCGCCAG‑AAGCGAGGTCATACCCGTCAGGCACGCCACCACGATAATCCGAATATACACGCCACTACCCGCGCCTTTTATTTACGATGGTTaaaaacaaccggaaaaga                 >  1:391473/1‑223 (MQ=255)                                                                                                                                                                                                                           ccGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGCTCGCGCGGATGCCGTCATGAAAAACACCCGCCGATCGATGCGCCAGCAAGCATTGCCA‑CCACGTCAGGCCCGCCCCCCCGATAACCTGAATATAACGATCACCAGACTGTCCTTTCATTTCAAATGGTTAAttcaacacggaaacacggggt            >  1:260430/1‑219 (MQ=255)                                                                                                                                                                                                                                   acaAAA‑CCAATGCCCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAG‑AAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTg     <  2:833196/238‑1 (MQ=255)                                                                                                                                                                                                                                     aaaa‑CCAATGCCCAGACCAAAAGCGATACCCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGGCATGAAAAACAGCCGCCGAGCGATGCGCCAG‑AAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTc  <  2:703296/239‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                     GGCGAGCTTAATTCCCCCAGGCTACCTAATACATCCACCGGCAGCGCGGTCAGCAGCTGGTTTACTGGCAACAGGCAAGTAAGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGT‑AAAAGAGATCCCCACCGAGGCCACAAAA‑CCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATAGCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAG‑AAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTC  >  NC_000913/3506928‑3507393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑