Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,065,570	T→A	14.8%	T66S (ACC→TCC)	yihO ←	putative sulphoquinovose importer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,065,570	0	T	A	14.8%	159.3 / 2.3	77	T66S (ACC→TCC)	yihO	putative sulphoquinovose importer
Reads supporting (aligned to +/- strand):  ref base T (43/18);  new base A (2/10);  total (48/29)
Fisher's exact test for biased strand distribution p-value = 7.61e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

AATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAAACTTCGCC‑ACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTAT  >  NC_000913/4065334‑4065808                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    aaTTGCCGGGATCAGCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGC‑AACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑Taaa                                                                                                                                                                                                                                                  <  2:502888/239‑1 (MQ=255)  aTTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGC‑Taa                                                                                                                                                                                                                                                   >  2:482376/1‑238 (MQ=255)    ttccGGGATCATCGCCCCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAc‑g                                                                                                                                                                                                                                               <  2:221190/237‑1 (MQ=255)              atcGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCACTATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTaaaa                                                                                                                                                                                                                                      >  2:115847/1‑237 (MQ=255)                    ccATACGAGCAGTTCAACAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAAACTTCGc                                                                                                                                                                                                                              <  2:614912/239‑1 (MQ=255)                          gAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTAGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAAACTTCGCC‑ACCAg                                                                                                                                                                                                                       <  2:664544/239‑1 (MQ=255)                            gCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTAAGCTTATCGG‑TAAAC‑C‑CGGTAAAAAACTTCGCC‑ACCCG‑aa                                                                                                                                                                                                                    >  2:799733/1‑239 (MQ=255)                            gCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGATGGCACTAAAA‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGACCCAATATTTTT‑CCGCAGG‑ACGAGTAAAA‑AA‑CC‑GGGTAGCTAACCGG‑AAAAA‑A‑CGGTAAAAAACTTACCC‑AGCAG‑aa                                                                                                                                                                                                                    >  1:33682/1‑239 (MQ=255)                             cAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAAACTTCGCC‑ACCAG‑aaa                                                                                                                                                                                                                   >  2:203950/1‑239 (MQ=255)                               gTTCATCAGACTGTATGAGAGTCCAAACCTCATAAACAGCGCGATGGCAATCGTCGTTTTCACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGCGCGTTGCAA‑TTAACGCAGCCGGAACCGCTG‑CATATACATTAAAGGGACGGAATTTGCCTT‑T‑AAGCCCAATCTTTTT‑TCTCCAG‑TCGAGGAAAA‑AT‑CC‑AGTGAGGATACCTG‑TAACA‑C‑AGGTAAAAAAATTCCCC‑AACAC‑aaaaa                                                                                                                                                                                                                 >  1:828693/1‑237 (MQ=255)                               gTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGACAATCGTCGTTTTAACCGGCAAACAAA‑AGGAGGTGGCGCTAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCGG‑CATATAAAATAACGGGCCGAAATTTGCCTA‑T‑AGGCCCAAAATTTTT‑CCACGAG‑TCGAGGAAAA‑AT‑CC‑GGTGAACATTACAG‑AAAAA‑G‑AGGAaaaaaaatacaccaaccaaaaaa                                                                                                                                                                                                                   >  1:453070/1‑222 (MQ=255)                                  catcaGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAAAATTCGCC‑ACCAa‑aaaaaaaa                                                                                                                                                                                                              >  1:968819/1‑234 (MQ=255)                                          tGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATCAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCTTATCGG‑TAAAC‑G‑CGGTAAAAAACTTCCCC‑ACCAG‑AAAGATAATCCAGccc                                                                                                                                                                                                      >  2:646725/1‑238 (MQ=255)                                          tGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAAACTTCGCC‑ACCAA‑AAAGAAAATCCAGCCa                                                                                                                                                                                                      >  2:303756/1‑239 (MQ=255)                                            tATGAAGGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAAACTTCGCC‑ACCAG‑AAAGATAATCCCGCCATa                                                                                                                                                                                                    >  2:855563/1‑239 (MQ=255)                                            tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATACCGG‑TAAAA‑C‑CGGTAAAAAACTTCCCC‑CCCAA‑AAAGAAAATCCCGCCaaa                                                                                                                                                                                                    >  1:790901/1‑237 (MQ=255)                                            tATGAAAGGCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTCACCGGCAACCAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGCCGACATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCTCTCG‑CCGCGGAAAA‑AT‑CC‑GGTACGACTATCGG‑TCAAC‑G‑CGGCAACAAACTTCGCC‑ACCTG‑AAAAGAAATCCCCCCaaa                                                                                                                                                                                                    >  1:235580/1‑237 (MQ=255)                                                    tCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAC‑AT‑CC‑GGGGAGCCTATCGG‑TAAAC‑G‑CGGTAAAAAACTTCGCC‑ACCAG‑AAAGATAATCCCGCCCTAGTAAGCAg                                                                                                                                                                                            >  2:685772/1‑239 (MQ=255)                                                      cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAACTAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAA‑G‑CGGTAAAAAACTTCGCC‑ACCAG‑AAAGATAATACCGCCAAAATAAgaaaaa                                                                                                                                                                                          >  1:206812/1‑234 (MQ=255)                                                      cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAAACTTCGCC‑ACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGc                                                                                                                                                                                          >  2:675078/1‑239 (MQ=255)                                                      cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAAACTTCGCC‑ACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGc                                                                                                                                                                                          >  2:617983/1‑239 (MQ=255)                                                      cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAAACTTCGCC‑ACAAG‑AAGGATAATCCCGCCATAGTAAGCAGGc                                                                                                                                                                                          >  2:144491/1‑239 (MQ=255)                                                      cAAACATCATAAACAGCGCGGTGGCAATCGACGTTTTAAACGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑CGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGACTATCGG‑GAAAA‑T‑CGGCAAACAACTTAGCC‑ACAAA‑AAAGAAAATCCAGCCaaaaaaaagagga                                                                                                                                                                                          >  1:259288/1‑227 (MQ=255)                                                        aaCATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGAGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCAGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CAGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTGAAAAACTTCGCC‑CCCAG‑AAAGATAATCCCTCCATAGGAAGCAGGC‑a                                                                                                                                                                                        >  2:338499/1‑238 (MQ=255)                                                            tcatAAACAGCGCGGTGGCAACCGTCGTTTTAACCGGCAAACAAA‑AGGTGGGGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAAACTTCGcc                                                                                                                                                                                                                             <  2:660485/200‑1 (MQ=255)                                                                  cacgggcggggaacagcgtcctTATAACCGAAAAAAAAA‑GGGTGGTGGGAAAAAAA‑GGGAGGGTTGAAA‑TTACCGCCGGCGGAACCGTTG‑CATAAAAAATAAAGGGACGAAATTGGCCTT‑T‑AGGCCCAATATTTTC‑CCGGGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAAACTTCGCC‑CCCAG‑AAAGATAATCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCa                                                                                                                                                                             <  1:203950/223‑1 (MQ=255)                                                                     gcgcGGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAAACTTCGCC‑ACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCg                                                                                                                                                                          >  2:844933/1‑239 (MQ=255)                                                                      cgcgGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAAACTTCGCC‑ACCAG‑AAAGAAAATCCCGCCATAGTAAGCAGCC‑ATCCCTAATTCATCgg                                                                                                                                                                         >  2:880498/1‑239 (MQ=255)                                                                       gcgGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAAACTTCGCC‑ACCGG‑AAAGATAATCCCGCCATAGTAAGCAGGA‑ATCCCTAATTCATCgga                                                                                                                                                                        >  2:401717/1‑238 (MQ=255)                                                                       gcgGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAAACTTCGCC‑ACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGt                                                                                                                                                                        >  2:679296/1‑239 (MQ=255)                                                                              cAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAAACTTCGCC‑ACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAAt                                                                                                                                                                 >  2:701408/1‑239 (MQ=255)                                                                                  tcgttttttCAACGGCAAACAAAGAGGTGGTGG‑AAAAAAA‑TGGGATGTTGAAA‑AAAACCGCCCCGGAAACAGTGCCATATAAAAAAAGGAAACGAATTGTGCCTT‑T‑AGGCACAATTTTTTA‑CCGCAGG‑CCCAGAAAAA‑AT‑CC‑GGTGAGTATACCGG‑TAAGC‑G‑GATAAAAAAACTTCACC‑ACAAG‑AAAGAAAAACCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTg                                                                                                                                                              <  1:844933/234‑1 (MQ=255)                                                                                   tccttttaccccGGCAAAAAGG‑GGGTGGTGCCTATAGAC‑TGACGCGTGGAAA‑TTGGCCTCGGCAGACCCGTGG‑CATAAAAAATAAAGAGACGTAATGTGCGTT‑T‑AGGCCCAATTTTATT‑CCGCGAG‑TCGATTAAAA‑TT‑CG‑TGTGAGCACTTCGA‑TAAAG‑G‑AGGTAAAAACCTCCACC‑AGAGC‑AAATATAAACCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTgag                                                                                                                                                            <  1:401717/229‑1 (MQ=255)                                                                                     cgtTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAC‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGGG‑TCGAGAAAAA‑AT‑CC‑GGGGAGCATAACGG‑CAACC‑G‑CGGTAAAAAACTTCCCC‑AACAG‑AAAGCTAATCCCGCCAT‑TTAAACAGCCAACCCCAAATTCATCGGAAAAAAATTTgaggg                                                                                                                                                          >  1:861753/1‑238 (MQ=255)                                                                                             cccgaaaacaaa‑AGGGTGGGGGGAAAAACTTGGACCTTTAAAA‑TTAACGCCGCCGGACCCGTGA‑AATAAAAAAAAAAGGGACAGAATTTGCTTT‑T‑AGGCCAAATATTTTT‑CGGGGAG‑CAAATAAAAA‑AT‑CG‑GGGGAGTATATCGG‑AAAAC‑G‑CGGAAAAAATCTCCGCC‑ACAAG‑AAAGATAACCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACag                                                                                                                                                   <  1:126601/234‑1 (MQ=255)                                                                                             ccGGGAAAAAAA‑AGGGGGTGGGGAAAAAA‑AGGGGTGGTGCAATTTACCGCGGCCGGAACCTATG‑TATAAAAAAAAAAGGGAAAAATTTTGCCTT‑T‑A‑GCACAAAATTTTTCCCGGGAG‑TCG‑GTAAAAGAC‑CC‑GGTGAGCATTGTGA‑AAAAC‑G‑GGGAAAAAAATCTCGCC‑ACCAG‑AAAAAAAACCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACag                                                                                                                                                   <  1:880498/239‑1 (MQ=255)                                                                                                 caaacaaa‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAAACTTCGCC‑ACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTCTAAAATTTCAGGAGATCCACAGTa                                                                                                                                               >  2:346972/1‑238 (MQ=255)                                                                                                               gtggCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAAACTTCGCC‑ACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGAACCGATGCAGAACAt                                                                                                                                 >  1:161833/1‑239 (MQ=255)                                                                                                                              gAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAAACTTCGCC‑ACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCCTCGGCATAAAATTTGAGGAAATACCAAGGACCGAGTCAAAGAATTAAAATCAAAcag                                                                                                                    >  1:526505/1‑236 (MQ=255)                                                                                                                                  gtgtaaa‑TTAACGCCGCGAGACCCGATG‑AATAAAAAAAAAAGGGAC‑AAAATTGCCTT‑TAAGGCCAAATTTTTTT‑CCGCGAG‑GCGATAAAAA‑AT‑CC‑GGTGAGCATTTCGG‑TAAAC‑G‑CGGTAAAAAACTCCGCC‑ACAAA‑AAAGATAATCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGt                                                                                                                <  2:396458/233‑1 (MQ=255)                                                                                                                                  ctttgaataaagccccgcggGACCCGTCG‑GATAAAAAAAAAAGGGAAG‑AATTTGCCTTAT‑AGGCCAAATAGTTGC‑CCGGAAG‑AGGAGTAAAA‑CT‑‑C‑GGGGGGTAATTCGGTTAAAC‑G‑GGAAAAAAAATTTCGCC‑ACCAG‑AAAGAAAAGCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACg                                                                                                                 <  1:701408/224‑1 (MQ=255)                                                                                                                                   tttaaatttacccccgcggGACCCGCTG‑AATATAAAAAAAAGGGACGAATTTTTCCGT‑T‑AGCCCAAATTTTTTC‑CCGCGTG‑TCGAGAAAAA‑AG‑CC‑GGTGAGAATATCGG‑TAACC‑G‑CGAAAAAAAACTCCGCC‑ACCAG‑AAATATACTCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGt                                                                                                                <  1:679296/225‑1 (MQ=255)                                                                                                                                   ttGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑T‑CGGTAAAAAACTTCGCC‑ACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGAATAAAATTTGCGGAGATACAGAGAACCGATGCAGAGCATTAAATTCGAAACGACGTc                                                                                                               >  1:474700/1‑238 (MQ=255)                                                                                                                                   ttGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑CAAAC‑G‑CGGTAAAAAACTTCGCC‑ACCAG‑AAAGATAATCCCGCCTTAGTAAGCAGGC‑ATCCCTAATTCCTCGGTATAAAATTTGAGTAGCTACAGAGTCCCGCTCCAGAGCATTAAATTAAAACCAACggg                                                                                                               >  1:781368/1‑236 (MQ=255)                                                                                                                                     gCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAAACTTCGCC‑ACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAAGGCATTAAATTCGAACCGACGTAGc                                                                                                             >  2:253255/1‑238 (MQ=255)                                                                                                                                            aaCGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGGG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAAACTTCGCC‑ACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATAAAAAGTACCAAGGCAAAGAATTAAATTCAAACCGACGGGGCccaaacc                                                                                                      >  1:396458/1‑239 (MQ=255)                                                                                                                                                                   aaaaaaaaaaGGGAAAAATTTCGCCTT‑T‑AGCCAAAATTTTTCC‑CCGGGAG‑TCAAAAAAAA‑AC‑CC‑GGTGAGCTTATCGT‑AAACC‑G‑CGAAAAAAACCTTCGCC‑ACCAA‑AAAGATAACCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAGCCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGa                                                                                <  2:18276/237‑1 (MQ=255)                                                                                                                                                                     aaaatgaaGTGACAAATTTTGTATG‑T‑AGCCCATATTTGTTG‑CCGAGAG‑TCGAGTAAAA‑CT‑GC‑GGTGGAAATTAAGA‑TACAC‑G‑CGATAAAAAGAGTCGCA‑ACCAA‑AAAGATATACCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGAtt                                                                              <  1:346972/239‑1 (MQ=255)                                                                                                                                                                     aaaataaaGGGACAAAATTTGCTTT‑T‑AGCCCCAATTTTTTT‑CCGGGAG‑TCGAGAAAAA‑AC‑CC‑GGTGAGCATATCGG‑AAAAC‑G‑GGGAAAAAACCTTCCCC‑CCCAG‑AAAGATAATCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGAtt                                                                              <  2:790901/239‑1 (MQ=255)                                                                                                                                                                     aaaaaaagGGGACAAATTGTTCCTT‑T‑GGCCTAAATTTTTTA‑CCGAGAG‑GTGAAAAAAA‑AT‑CC‑GGGGTGTAAAACGG‑TAAAC‑G‑GGGAAAAAACCTCCGCA‑CCAAG‑AAAGTAAACCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGAtt                                                                              <  1:144491/239‑1 (MQ=255)                                                                                                                                                                      aaaaaaaGGGAAATAATTTGTTTT‑T‑GGGCCCAAATATTTT‑CCAGAAG‑TCAGGAAAAA‑AA‑CC‑GGTGAGTAACACGG‑AAAAA‑GTGGGAAAAAAACTGCGCC‑ACAAA‑AAAAAAAATCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGAtt                                                                              <  1:503544/239‑1 (MQ=255)                                                                                                                                                                          aaaGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAAACTTCGCC‑ACCAG‑AAAGATAATCCCGCCATAGTAAGCAGTC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAAAGTACCGAt                                                                                                                                          >  2:510331/1‑174 (MQ=255)                                                                                                                                                                          aaaGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAAACTTCGCC‑ACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCg                                                                                                                                                                          >  2:956731/1‑142 (MQ=255)                                                                                                                                                                          aaaGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAAACTTCGCC‑ACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAGCCGACGTCCCCCATACCATAGGGGATTTTTTCCCGCAAAATAAGtt                                                                         >  1:18276/1‑239 (MQ=255)                                                                                                                                                                            aGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAAACTTCGCC‑ACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATaaaa                                                                                                                                                                  >  2:411182/1‑148 (MQ=255)                                                                                                                                                                                aCGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAAACTTCGCC‑ACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAAAGCATTAAATTCGAACCCACGTCGCCCATACCCTAGGCGATTTTTTCCCGCAGATTCCTTTTTAAt                                                                    >  1:69020/1‑238 (MQ=255)                                                                                                                                                                                 cGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAAACTTCGCC‑ACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCccata                                                                                                        >  2:342531/1‑201 (MQ=255)                                                                                                                                                                                 cGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAAACTTCGCC‑ACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGACCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAAATTCAGTTTTaaatt                                                                  >  1:329211/1‑236 (MQ=255)                                                                                                                                                                                 cGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAAACTTCGCC‑ACCAG‑AAAGATAATCCAGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGGATAAAATTTGACGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGGCGCCCATACCAAAGGCGATTTTTTCCCGCACATTCAGTTTTaaag                                                                   >  1:828655/1‑236 (MQ=255)                                                                                                                                                                                   aaaTTTGCCTT‑T‑AGGCCAAAT‑TTTTT‑CCGGCAGATAGAAAAAAA‑CC‑CC‑GGTGAGCATTTCGG‑AAAAC‑G‑CGATAAAAACCTTCGCC‑ACCAA‑AAAGATAATCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCa                                                                <  2:968819/239‑1 (MQ=255)                                                                                                                                                                                     tttttCCTT‑G‑AGGCCCAAATTTTTT‑CCGCGGG‑TCGGATAAAA‑AT‑CC‑GGGGAGCATATCGG‑AAAAC‑G‑CGGTAAAAACCTTCGCC‑ACAAA‑AAAGATAACCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCa                                                                <  2:728637/236‑1 (MQ=255)                                                                                                                                                                                                 aGGCCAAATTTTTAT‑CCGCGAG‑GCGAGAAAAA‑AT‑CC‑GGGGACAATTGCGG‑AAAAG‑G‑GGGAAAAAACCTGCGCC‑AGCAG‑AAAGATAACCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGt                                                    <  2:526505/239‑1 (MQ=255)                                                                                                                                                                                                  tgCCCAATTTTTTT‑CCCGGTG‑TTGCGAATAA‑ATCCC‑GTTGACCATATCGG‑TACAC‑G‑GTAAAAAAACCTTCGCC‑ACAAA‑AAAGAAAACCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGt                                                    <  2:151944/238‑1 (MQ=255)                                                                                                                                                                                                    cccAATATTTTG‑CCGGGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAAACTTCGCC‑ACAAG‑AAAGATAATCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCa                                                  <  2:329211/238‑1 (MQ=255)                                                                                                                                                                                                       aaTATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAACCTTCGCC‑ACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTCTAAAATTTGAGGAGATACAGAGTACCGATGAATAGCATTACATTCGAACCGACGCCGCCCCTACCATAGGCGATTTTTTCCCCAACATTCAGTTTTATCTGCAGGCGATTATGGtacaaac                                              >  1:168383/1‑233 (MQ=255)                                                                                                                                                                                                              ttcccGCGAG‑TCGAGTAAAA‑CC‑GC‑GGTGAGAATAGCGA‑AAACC‑G‑CGGAAAAAACCTCCGCC‑ACCAA‑AAAGATAACCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCa                                        <  2:933724/236‑1 (MQ=255)                                                                                                                                                                                                              tt‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAAACTTCGCC‑ACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCCCAGTTTCAGTTTTAATTTCAGGGGATTATGGTCaaaataaatca                                        >  1:54684/1‑228 (MQ=255)                                                                                                                                                                                                                  cccgtgggtcgtataaa‑aT‑CCGGGGGAGTATATCGG‑TAAGC‑T‑CAGAAAACAGCTTCCCA‑ATAAA‑AAAAATAACCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATTCCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACt                                      <  1:482376/225‑1 (MQ=255)                                                                                                                                                                                                                    cgag‑tcgagtAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAAACTTCGCC‑ACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGCTTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCtt                                  >  2:852460/1‑239 (MQ=255)                                                                                                                                                                                                                    cgag‑tcgagtAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAAACTTCGCC‑ACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAAAGAACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAAATTCCGTGGGTTATGGTCAGAAAAAAACACCCCtt                                  >  1:933724/1‑239 (MQ=255)                                                                                                                                                                                                                                        c‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAAACTTCGCC‑ACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAAt                                                                    >  2:338167/1‑188 (MQ=255)                                                                                                                                                                                                                                        c‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAAACTTCGCC‑ACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGCTTATGGTCAGACATAATCACTCCTTTTAACGCCCCGCAGGGc                 >  2:147277/1‑239 (MQ=255)                                                                                                                                                                                                                                          ggtgccaataaCGG‑TATCC‑G‑CGGTAAAAACCTGC‑CC‑CCCAGAAAAGATAATCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGATTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGcg                <  2:206812/232‑1 (MQ=255)                                                                                                                                                                                                                                           gtagagatatCGG‑AAAAC‑G‑GGGTAAAAAACGTCGCC‑CAGAA‑AAAAATAATCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATGAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGcg                <  2:861753/232‑1 (MQ=255)                                                                                                                                                                                                                                                cATATCGG‑TAAAC‑G‑CGGTAAAAAACTTCGCC‑ACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATAAGTCCTTTTAAAGCCCCGCAGGACTCCGttt          >  2:130714/1‑239 (MQ=255)                                                                                                                                                                                                                                                  acgcGG‑TAAAC‑‑‑CGGGAAAAAACTTCCCCAACAAA‑AAAGAAAACCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTttat        <  2:474700/236‑1 (MQ=255)                                                                                                                                                                                                                                                   tacGG‑GAAACGG‑CGGAAAAAACCTCCGCC‑ACAAG‑AAAAAAAATCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCAGCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTttat        <  2:69020/237‑1 (MQ=255)                                                                                                                                                                                                                                                         tAAAC‑G‑CGGTAAAAAACTTCGCC‑ACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACACCTTATAACGCCCCGCAGGGCGTCGTtttttattat  >  1:657078/1‑239 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                    AATTGCCGGGATCATCGCACCATACGAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAAACTTCGCC‑ACCAG‑AAAGATAATCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTAT  >  NC_000913/4065334‑4065808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑