Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,065,595	C→A	18.2%	L57L (CTG→CTT)	yihO ←	putative sulphoquinovose importer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,065,595	0	C	A	18.2%	158.7 / 5.7	78	L57L (CTG→CTT)	yihO	putative sulphoquinovose importer
Reads supporting (aligned to +/- strand):  ref base C (48/14);  new base A (2/13);  total (50/28)
Fisher's exact test for biased strand distribution p-value = 7.25e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAA‑ACTTCGCC‑ACCAG‑AAAGATAA‑TCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCGTGCGTTTACGGG  >  NC_000913/4065359‑4065827                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                            gAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTAGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAA‑ACTTCGCC‑ACCAg                                                                                                                                                                                                                                           <  2:664544/239‑1 (MQ=255)   gCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTAAGCTTATCGG‑TAAAC‑C‑CGGTAAAAA‑ACTTCGCC‑ACCCG‑aa                                                                                                                                                                                                                                        >  2:799733/1‑239 (MQ=255)   gCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGATGGCACTAAAA‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGACCCAATATTTTT‑CCGCAGG‑ACGAGTAAAA‑AA‑CC‑GGGTAGCTAACCGG‑AAAAA‑A‑CGGTAAAAA‑ACTTACCC‑AGCAG‑aa                                                                                                                                                                                                                                        >  1:33682/1‑239 (MQ=255)    cAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAA‑ACTTCGCC‑ACCAG‑aaa                                                                                                                                                                                                                                       >  2:203950/1‑239 (MQ=255)      gTTCATCAGACTGTATGAGAGTCCAAACCTCATAAACAGCGCGATGGCAATCGTCGTTTTCACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGCGCGTTGCAA‑TTAACGCAGCCGGAACCGCTG‑CATATACATTAAAGGGACGGAATTTGCCTT‑T‑AAGCCCAATCTTTTT‑TCTCCAG‑TCGAGGAAAA‑AT‑CC‑AGTGAGGATACCTG‑TAACA‑C‑AGGTAAAAA‑AATTCCCC‑AACAC‑aaaaa                                                                                                                                                                                                                                     >  1:828693/1‑237 (MQ=255)         catcaGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAA‑AATTCGCC‑ACCAa‑aaaaaaaa                                                                                                                                                                                                                                  >  1:968819/1‑234 (MQ=255)                 tGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATCAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCTTATCGG‑TAAAC‑G‑CGGTAAAAA‑ACTTCCCC‑ACCAG‑AAAGATAA‑TCCAGccc                                                                                                                                                                                                                         >  2:646725/1‑238 (MQ=255)                 tGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAA‑ACTTCGCC‑ACCAA‑AAAGAAAA‑TCCAGCCa                                                                                                                                                                                                                         >  2:303756/1‑239 (MQ=255)                   tATGAAGGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAA‑ACTTCGCC‑ACCAG‑AAAGATAA‑TCCCGCCATa                                                                                                                                                                                                                       >  2:855563/1‑239 (MQ=255)                   tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATACCGG‑TAAAA‑C‑CGGTAAAAA‑ACTTCCCC‑CCCAA‑AAAGAAAA‑TCCCGCCaaa                                                                                                                                                                                                                       >  1:790901/1‑237 (MQ=255)                   tATGAAAGGCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTCACCGGCAACCAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGCCGACATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCTCTCG‑CCGCGGAAAA‑AT‑CC‑GGTACGACTATCGG‑TCAAC‑G‑CGGCAACAA‑ACTTCGCC‑ACCTG‑AAAAGAAA‑TCCCCCCaaa                                                                                                                                                                                                                       >  1:235580/1‑237 (MQ=255)                           tCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAC‑AT‑CC‑GGGGAGCCTATCGG‑TAAAC‑G‑CGGTAAAAA‑ACTTCGCC‑ACCAG‑AAAGATAA‑TCCCGCCCTAGTAAGCAg                                                                                                                                                                                                               >  2:685772/1‑239 (MQ=255)                             cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAACTAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAA‑G‑CGGTAAAAA‑ACTTCGCC‑ACCAG‑AAAGATAA‑TACCGCCAAAATAAgaaaaa                                                                                                                                                                                                             >  1:206812/1‑234 (MQ=255)                             cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAA‑ACTTCGCC‑ACCAG‑AAAGATAA‑TCCCGCCATAGTAAGCAGGc                                                                                                                                                                                                             >  2:675078/1‑239 (MQ=255)                             cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAA‑ACTTCGCC‑ACCAG‑AAAGATAA‑TCCCGCCATAGTAAGCAGGc                                                                                                                                                                                                             >  2:617983/1‑239 (MQ=255)                             cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAA‑ACTTCGCC‑ACAAG‑AAGGATAA‑TCCCGCCATAGTAAGCAGGc                                                                                                                                                                                                             >  2:144491/1‑239 (MQ=255)                             cAAACATCATAAACAGCGCGGTGGCAATCGACGTTTTAAACGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑CGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGACTATCGG‑GAAAA‑T‑CGGCAAACA‑ACTTAGCC‑ACAAA‑AAAGAAAA‑TCCAGCCaaaaaaaagagga                                                                                                                                                                                                             >  1:259288/1‑227 (MQ=255)                               aaCATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGAGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCAGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CAGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTGAAAA‑ACTTCGCC‑CCCAG‑AAAGATAA‑TCCCTCCATAGGAAGCAGGC‑a                                                                                                                                                                                                           >  2:338499/1‑238 (MQ=255)                                         cacgggcggggaacagcgtcctTATAACCGAAAAAAAAA‑GGGTGGTGGGAAAAAAA‑GGGAGGGTTGAAA‑TTACCGCCGGCGGAACCGTTG‑CATAAAAAATAAAGGGACGAAATTGGCCTT‑T‑AGGCCCAATATTTTC‑CCGGGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAA‑ACTTCGCC‑CCCAG‑AAAGATAA‑TCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCa                                                                                                                                                                                                <  1:203950/223‑1 (MQ=255)                                            gcgcGGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAA‑ACTTCGCC‑ACCAG‑AAAGATAA‑TCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCg                                                                                                                                                                                             >  2:844933/1‑239 (MQ=255)                                             cgcgGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAA‑ACTTCGCC‑ACCAG‑AAAGAAAA‑TCCCGCCATAGTAAGCAGCC‑ATCCCTAATTCATCgg                                                                                                                                                                                            >  2:880498/1‑239 (MQ=255)                                              gcgGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAA‑ACTTCGCC‑ACCGG‑AAAGATAA‑TCCCGCCATAGTAAGCAGGA‑ATCCCTAATTCATCgga                                                                                                                                                                                           >  2:401717/1‑238 (MQ=255)                                              gcgGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAA‑ACTTCGCC‑ACCAG‑AAAGATAA‑TCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGt                                                                                                                                                                                           >  2:679296/1‑239 (MQ=255)                                                     cAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAA‑ACTTCGCC‑ACCAG‑AAAGATAA‑TCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAAt                                                                                                                                                                                    >  2:701408/1‑239 (MQ=255)                                                         tcgttttttCAACGGCAAACAAAGAGGTGGTGG‑AAAAAAA‑TGGGATGTTGAAA‑AAAACCGCCCCGGAAACAGTGCCATATAAAAAAAGGAAACGAATTGTGCCTT‑T‑AGGCACAATTTTTTA‑CCGCAGG‑CCCAGAAAAA‑AT‑CC‑GGTGAGTATACCGG‑TAAGC‑G‑GATAAAAAA‑ACTTCACC‑ACAAG‑AAAGAAAA‑ACCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTg                                                                                                                                                                                 <  1:844933/234‑1 (MQ=255)                                                          tccttttaccccGGCAAAAAGG‑GGGTGGTGCCTATAGAC‑TGACGCGTGGAAA‑TTGGCCTCGGCAGACCCGTGG‑CATAAAAAATAAAGAGACGTAATGTGCGTT‑T‑AGGCCCAATTTTATT‑CCGCGAG‑TCGATTAAAA‑TT‑CG‑TGTGAGCACTTCGA‑TAAAG‑G‑AGGTAAAAA‑CCTCCACC‑AGAGC‑AAATATAA‑ACCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTgag                                                                                                                                                                               <  1:401717/229‑1 (MQ=255)                                                            cgtTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAC‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGGG‑TCGAGAAAAA‑AT‑CC‑GGGGAGCATAACGG‑CAACC‑G‑CGGTAAAAA‑ACTTCCCC‑AACAG‑AAAGCTAA‑TCCCGCCAT‑TTAAACAGCCAACCCCAAATTCATCGGAAAAAAATTTgaggg                                                                                                                                                                             >  1:861753/1‑238 (MQ=255)                                                                    cccgaaaacaaa‑AGGGTGGGGGGAAAAACTTGGACCTTTAAAA‑TTAACGCCGCCGGACCCGTGA‑AATAAAAAAAAAAGGGACAGAATTTGCTTT‑T‑AGGCCAAATATTTTT‑CGGGGAG‑CAAATAAAAA‑AT‑CG‑GGGGAGTATATCGG‑AAAAC‑G‑CGGAAAAAA‑TCTCCGCC‑ACAAG‑AAAGATAA‑CCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACag                                                                                                                                                                      <  1:126601/234‑1 (MQ=255)                                                                    ccGGGAAAAAAA‑AGGGGGTGGGGAAAAAA‑AGGGGTGGTGCAATTTACCGCGGCCGGAACCTATG‑TATAAAAAAAAAAGGGAAAAATTTTGCCTT‑T‑A‑GCACAAAATTTTTCCCGGGAG‑TCG‑GTAAAAGAC‑CC‑GGTGAGCATTGTGA‑AAAAC‑G‑GGGAAAAAA‑ATCTCGCC‑ACCAG‑AAAAAAAA‑CCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACag                                                                                                                                                                      <  1:880498/239‑1 (MQ=255)                                                                        caaacaaa‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAA‑ACTTCGCC‑ACCAG‑AAAGATAA‑TCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTCTAAAATTTCAGGAGATCCACAGTa                                                                                                                                                                  >  2:346972/1‑238 (MQ=255)                                                                                      gtggCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAA‑ACTTCGCC‑ACCAG‑AAAGATAA‑TCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGAACCGATGCAGAACAt                                                                                                                                                    >  1:161833/1‑239 (MQ=255)                                                                                                     gAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAA‑ACTTCGCC‑ACCAG‑AAAGATAA‑TCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCCTCGGCATAAAATTTGAGGAAATACCAAGGACCGAGTCAAAGAATTAAAATCAAAcag                                                                                                                                       >  1:526505/1‑236 (MQ=255)                                                                                                         gtgtaaa‑TTAACGCCGCGAGACCCGATG‑AATAAAAAAAAAAGGGAC‑AAAATTGCCTT‑TAAGGCCAAATTTTTTT‑CCGCGAG‑GCGATAAAAA‑AT‑CC‑GGTGAGCATTTCGG‑TAAAC‑G‑CGGTAAAAA‑ACTCCGCC‑ACAAA‑AAAGATAA‑TCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGt                                                                                                                                   <  2:396458/233‑1 (MQ=255)                                                                                                         ctttgaataaagccccgcggGACCCGTCG‑GATAAAAAAAAAAGGGAAG‑AATTTGCCTTAT‑AGGCCAAATAGTTGC‑CCGGAAG‑AGGAGTAAAA‑CT‑‑C‑GGGGGGTAATTCGGTTAAAC‑G‑GGAAAAAAA‑ATTTCGCC‑ACCAG‑AAAGAAAA‑GCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACg                                                                                                                                    <  1:701408/224‑1 (MQ=255)                                                                                                          tttaaatttacccccgcggGACCCGCTG‑AATATAAAAAAAAGGGACGAATTTTTCCGT‑T‑AGCCCAAATTTTTTC‑CCGCGTG‑TCGAGAAAAA‑AG‑CC‑GGTGAGAATATCGG‑TAACC‑G‑CGAAAAAAA‑ACTCCGCC‑ACCAG‑AAATATAC‑TCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGt                                                                                                                                   <  1:679296/225‑1 (MQ=255)                                                                                                          ttGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑T‑CGGTAAAAA‑ACTTCGCC‑ACCAG‑AAAGATAA‑TCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGAATAAAATTTGCGGAGATACAGAGAACCGATGCAGAGCATTAAATTCGAAACGACGTc                                                                                                                                  >  1:474700/1‑238 (MQ=255)                                                                                                          ttGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑CAAAC‑G‑CGGTAAAAA‑ACTTCGCC‑ACCAG‑AAAGATAA‑TCCCGCCTTAGTAAGCAGGC‑ATCCCTAATTCCTCGGTATAAAATTTGAGTAGCTACAGAGTCCCGCTCCAGAGCATTAAATTAAAACCAACggg                                                                                                                                  >  1:781368/1‑236 (MQ=255)                                                                                                            gCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAA‑ACTTCGCC‑ACCAG‑AAAGATAA‑TCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAAGGCATTAAATTCGAACCGACGTAGc                                                                                                                                >  2:253255/1‑238 (MQ=255)                                                                                                                   aaCGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGGG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAA‑ACTTCGCC‑ACCAG‑AAAGATAA‑TCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATAAAAAGTACCAAGGCAAAGAATTAAATTCAAACCGACGGGGCccaaacc                                                                                                                         >  1:396458/1‑239 (MQ=255)                                                                                                                                          aaaaaaaaaaGGGAAAAATTTCGCCTT‑T‑AGCCAAAATTTTTCC‑CCGGGAG‑TCAAAAAAAA‑AC‑CC‑GGTGAGCTTATCGT‑AAACC‑G‑CGAAAAAAA‑CCTTCGCC‑ACCAA‑AAAGATAA‑CCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAGCCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGa                                                                                                   <  2:18276/237‑1 (MQ=255)                                                                                                                                            aaaatgaaGTGACAAATTTTGTATG‑T‑AGCCCATATTTGTTG‑CCGAGAG‑TCGAGTAAAA‑CT‑GC‑GGTGGAAATTAAGA‑TACAC‑G‑CGATAAAAA‑GAGTCGCA‑ACCAA‑AAAGATAT‑ACCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGAtt                                                                                                 <  1:346972/239‑1 (MQ=255)                                                                                                                                            aaaataaaGGGACAAAATTTGCTTT‑T‑AGCCCCAATTTTTTT‑CCGGGAG‑TCGAGAAAAA‑AC‑CC‑GGTGAGCATATCGG‑AAAAC‑G‑GGGAAAAAA‑CCTTCCCC‑CCCAG‑AAAGATAA‑TCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGAtt                                                                                                 <  2:790901/239‑1 (MQ=255)                                                                                                                                            aaaaaaagGGGACAAATTGTTCCTT‑T‑GGCCTAAATTTTTTA‑CCGAGAG‑GTGAAAAAAA‑AT‑CC‑GGGGTGTAAAACGG‑TAAAC‑G‑GGGAAAAAA‑CCTCCGCA‑CCAAG‑AAAGTAAA‑CCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGAtt                                                                                                 <  1:144491/239‑1 (MQ=255)                                                                                                                                             aaaaaaaGGGAAATAATTTGTTTT‑T‑GGGCCCAAATATTTT‑CCAGAAG‑TCAGGAAAAA‑AA‑CC‑GGTGAGTAACACGG‑AAAAA‑GTGGGAAAAAA‑ACTGCGCC‑ACAAA‑AAAAAAAA‑TCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGAtt                                                                                                 <  1:503544/239‑1 (MQ=255)                                                                                                                                                 aaaGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAA‑ACTTCGCC‑ACCAG‑AAAGATAA‑TCCCGCCATAGTAAGCAGTC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAAAGTACCGAt                                                                                                                                                             >  2:510331/1‑174 (MQ=255)                                                                                                                                                 aaaGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAA‑ACTTCGCC‑ACCAG‑AAAGATAA‑TCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCg                                                                                                                                                                                             >  2:956731/1‑142 (MQ=255)                                                                                                                                                 aaaGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAA‑ACTTCGCC‑ACCAG‑AAAGATAA‑TCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAGCCGACGTCCCCCATACCATAGGGGATTTTTTCCCGCAAAATAAGtt                                                                                            >  1:18276/1‑239 (MQ=255)                                                                                                                                                   aGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAA‑ACTTCGCC‑ACCAG‑AAAGATAA‑TCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATaaaa                                                                                                                                                                                     >  2:411182/1‑148 (MQ=255)                                                                                                                                                       aCGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAA‑ACTTCGCC‑ACCAG‑AAAGATAA‑TCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAAAGCATTAAATTCGAACCCACGTCGCCCATACCCTAGGCGATTTTTTCCCGCAGATTCCTTTTTAAt                                                                                       >  1:69020/1‑238 (MQ=255)                                                                                                                                                        cGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAA‑ACTTCGCC‑ACCAG‑AAAGATAA‑TCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCccata                                                                                                                           >  2:342531/1‑201 (MQ=255)                                                                                                                                                        cGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAA‑ACTTCGCC‑ACCAG‑AAAGATAA‑TCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGACCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAAATTCAGTTTTaaatt                                                                                     >  1:329211/1‑236 (MQ=255)                                                                                                                                                        cGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAA‑ACTTCGCC‑ACCAG‑AAAGATAA‑TCCAGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGGATAAAATTTGACGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGGCGCCCATACCAAAGGCGATTTTTTCCCGCACATTCAGTTTTaaag                                                                                      >  1:828655/1‑236 (MQ=255)                                                                                                                                                          aaaTTTGCCTT‑T‑AGGCCAAAT‑TTTTT‑CCGGCAGATAGAAAAAAA‑CC‑CC‑GGTGAGCATTTCGG‑AAAAC‑G‑CGATAAAAA‑CCTTCGCC‑ACCAA‑AAAGATAA‑TCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCa                                                                                   <  2:968819/239‑1 (MQ=255)                                                                                                                                                            tttttCCTT‑G‑AGGCCCAAATTTTTT‑CCGCGGG‑TCGGATAAAA‑AT‑CC‑GGGGAGCATATCGG‑AAAAC‑G‑CGGTAAAAA‑CCTTCGCC‑ACAAA‑AAAGATAA‑CCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCa                                                                                   <  2:728637/236‑1 (MQ=255)                                                                                                                                                                        aGGCCAAATTTTTAT‑CCGCGAG‑GCGAGAAAAA‑AT‑CC‑GGGGACAATTGCGG‑AAAAG‑G‑GGGAAAAAA‑CCTGCGCC‑AGCAG‑AAAGATAA‑CCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGt                                                                       <  2:526505/239‑1 (MQ=255)                                                                                                                                                                         tgCCCAATTTTTTT‑CCCGGTG‑TTGCGAATAA‑ATCCC‑GTTGACCATATCGG‑TACAC‑G‑GTAAAAAAA‑CCTTCGCC‑ACAAA‑AAAGAAAA‑CCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGt                                                                       <  2:151944/238‑1 (MQ=255)                                                                                                                                                                           cccAATATTTTG‑CCGGGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAA‑ACTTCGCC‑ACAAG‑AAAGATAA‑TCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCa                                                                     <  2:329211/238‑1 (MQ=255)                                                                                                                                                                              aaTATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAA‑CCTTCGCC‑ACCAG‑AAAGATAA‑TCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTCTAAAATTTGAGGAGATACAGAGTACCGATGAATAGCATTACATTCGAACCGACGCCGCCCCTACCATAGGCGATTTTTTCCCCAACATTCAGTTTTATCTGCAGGCGATTATGGtacaaac                                                                 >  1:168383/1‑233 (MQ=255)                                                                                                                                                                                     ttcccGCGAG‑TCGAGTAAAA‑CC‑GC‑GGTGAGAATAGCGA‑AAACC‑G‑CGGAAAAAA‑CCTCCGCC‑ACCAA‑AAAGATAA‑CCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCa                                                           <  2:933724/236‑1 (MQ=255)                                                                                                                                                                                     tt‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAA‑ACTTCGCC‑ACCAG‑AAAGATAA‑TCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCCCAGTTTCAGTTTTAATTTCAGGGGATTATGGTCaaaataaatca                                                           >  1:54684/1‑228 (MQ=255)                                                                                                                                                                                         cccgtgggtcgtataaa‑aT‑CCGGGGGAGTATATCGG‑TAAGC‑T‑CAGAAAACA‑GCTTCCCA‑ATAAA‑AAAAATAA‑CCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATTCCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACt                                                         <  1:482376/225‑1 (MQ=255)                                                                                                                                                                                           cgag‑tcgagtAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAA‑ACTTCGCC‑ACCAG‑AAAGATAA‑TCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGCTTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCtt                                                     >  2:852460/1‑239 (MQ=255)                                                                                                                                                                                           cgag‑tcgagtAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAA‑ACTTCGCC‑ACCAG‑AAAGATAA‑TCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAAAGAACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAAATTCCGTGGGTTATGGTCAGAAAAAAACACCCCtt                                                     >  1:933724/1‑239 (MQ=255)                                                                                                                                                                                                               c‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAA‑ACTTCGCC‑ACCAG‑AAAGATAA‑TCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAAt                                                                                       >  2:338167/1‑188 (MQ=255)                                                                                                                                                                                                               c‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAA‑ACTTCGCC‑ACCAG‑AAAGATAA‑TCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGCTTATGGTCAGACATAATCACTCCTTTTAACGCCCCGCAGGGc                                    >  2:147277/1‑239 (MQ=255)                                                                                                                                                                                                                 ggtgccaataaCGG‑TATCC‑G‑CGGTAAAAA‑CCTGC‑CC‑CCCAGAAAAGATAA‑TCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGATTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGcg                                   <  2:206812/232‑1 (MQ=255)                                                                                                                                                                                                                  gtagagatatCGG‑AAAAC‑G‑GGGTAAAAA‑ACGTCGCC‑CAGAA‑AAAAATAA‑TCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATGAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGcg                                   <  2:861753/232‑1 (MQ=255)                                                                                                                                                                                                                     gggagattaggagtaaccgggaaaaaaa‑aCTTCGAC‑AACAA‑AAAATAAA‑TCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGGCCCGCAGGGcg                                   <  2:781368/215‑1 (MQ=255)                                                                                                                                                                                                                       cATATCGG‑TAAAC‑G‑CGGTAAAAA‑ACTTCGCC‑ACCAG‑AAAGATAA‑TCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATAAGTCCTTTTAAAGCCCCGCAGGACTCCGttt                             >  2:130714/1‑239 (MQ=255)                                                                                                                                                                                                                         acgcGG‑TAAAC‑‑‑CGGGAAAAA‑ACTTCCCCAACAAA‑AAAGAAAA‑CCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTttat                           <  2:474700/236‑1 (MQ=255)                                                                                                                                                                                                                          tacGG‑GAAACGG‑CGGAAAAAA‑CCTCCGCC‑ACAAG‑AAAAAAAA‑TCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCAGCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTttat                           <  2:69020/237‑1 (MQ=255)                                                                                                                                                                                                                            acagggaaagggaagaaaaaa‑aCTT‑GCC‑CCCAA‑AAAAATAACGCCCGTCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTttat                           <  1:675078/223‑1 (MQ=255)                                                                                                                                                                                                                             taatggaaaacgggTAAAAACGCTTCGCC‑AAAAG‑AAAGAGAA‑TCCCGCCAAAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTttat                           <  2:81499/227‑1 (MQ=255)                                                                                                                                                                                                                                tAAAC‑G‑CGGTAAAAA‑ACTTCGCC‑ACCAG‑AAAGATAA‑TCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACACCTTATAACGCCCCGCAGGGCGTCGTtttttattat                     >  1:657078/1‑239 (MQ=255)                                                                                                                                                                                                                                  aaC‑G‑CGGTAAAAA‑ACTTCGCC‑ACCAG‑AAAGATAA‑TCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGTTTAAGTTTTAATGTCAGTGGATAATGGTCAGACTTAATCACTCCTTTTACGGCCCCCCAGGGCGCCGTttttaattataa                   >  1:81499/1‑237 (MQ=255)                                                                                                                                                                                                                                          gTAAAAA‑ACTTCGCC‑ACCAG‑AAAGATAA‑TCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCGt             >  2:138760/1‑239 (MQ=255)                                                                                                                                                                                                                                           tAAAAA‑ACTTCGCC‑ACCAG‑AAAGATAA‑TCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACg                                               >  2:654522/1‑204 (MQ=255)                                                                                                                                                                                                                                                      cGCC‑ACCAG‑AAAGATAA‑TCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCGTGCGTTTACggg  >  2:920992/1‑239 (MQ=255)                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                            GAGCAGTTCATCAGACTGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAA‑AGGTGGTGGCGATAAAC‑TGGAGCGTTGCAA‑TTAACGCCGCCGGAACCGCTG‑CATATAAAATAAAGGGACGAAATTTGCCTT‑T‑AGGCCCAATATTTTT‑CCGCGAG‑TCGAGTAAAA‑AT‑CC‑GGTGAGCATATCGG‑TAAAC‑G‑CGGTAAAAA‑ACTTCGCC‑ACCAG‑AAAGATAA‑TCCCGCCATAGTAAGCAGGC‑ATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCGTGCGTTTACGGG  >  NC_000913/4065359‑4065827

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑