Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,065,603 T→A 18.6% I55F (ATC→TTC)  yihO ← putative sulphoquinovose importer

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,065,6030TA18.6% 142.2 / 4.6 74I55F (ATC→TTC) yihOputative sulphoquinovose importer
Reads supporting (aligned to +/- strand):  ref base T (40/18);  new base A (5/10);  total (45/29)
Fisher's exact test for biased strand distribution p-value = 1.70e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCGTGCGTTTACGGGCTTCT  >  NC_000913/4065375‑4065832
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                       
tGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATCAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCTTATCGGTAAACGCGGTAAAAAACTTCCCCACCAGAAAGATAATCCAGccc                                                                                                                                                                                                                              >  2:646725/1‑238 (MQ=255)
tGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAAAAAGAAAATCCAGCCa                                                                                                                                                                                                                              >  2:303756/1‑239 (MQ=255)
  tATGAAGGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATa                                                                                                                                                                                                                            >  2:855563/1‑239 (MQ=255)
  tATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATACCGGTAAAACCGGTAAAAAACTTCCCCCCCAAAAAGAAAATCCCGCCaaa                                                                                                                                                                                                                            >  1:790901/1‑237 (MQ=255)
  tATGAAAGGCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTCACCGGCAACCAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGCCGACATTTGCCTTTAGGCCCAATATTTTTCCTCTCGCCGCGGAAAAATCCGGTACGACTATCGGTCAACGCGGCAACAAACTTCGCCACCTGAAAAGAAATCCCCCCaaa                                                                                                                                                                                                                            >  1:235580/1‑237 (MQ=255)
          tCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAACATCCGGGGAGCCTATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCCTAGTAAGCAg                                                                                                                                                                                                                    >  2:685772/1‑239 (MQ=255)
            cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAACTAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAAAGCGGTAAAAAACTTCGCCACCAGAAAGATAATACCGCCAAAATAAgaaaaa                                                                                                                                                                                                                  >  1:206812/1‑234 (MQ=255)
            cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGc                                                                                                                                                                                                                  >  2:617983/1‑239 (MQ=255)
            cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGc                                                                                                                                                                                                                  >  2:675078/1‑239 (MQ=255)
            cAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACAAGAAGGATAATCCCGCCATAGTAAGCAGGc                                                                                                                                                                                                                  >  2:144491/1‑239 (MQ=255)
            cAAACATCATAAACAGCGCGGTGGCAATCGACGTTTTAAACGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTCGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGACTATCGGGAAAATCGGCAAACAACTTAGCCACAAAAAAGAAAATCCAGCCaaaaaaaagagga                                                                                                                                                                                                                  >  1:259288/1‑227 (MQ=255)
              aaCATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGAGGCGATAAACTGGAGCGTTGCAATTAACGCAGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCAGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTGAAAAACTTCGCCCCCAGAAAGATAATCCCTCCATAGGAAGCAGGCa                                                                                                                                                                                                                >  2:338499/1‑238 (MQ=255)
                        cacgggcggggaacagcgtcctTATAACCGAAAAAAAAAGGGTGGTGGGAAAAAAAGGGAGGGTTGAAATTACCGCCGGCGGAACCGTTGCATAAAAAATAAAGGGACGAAATTGGCCTTTAGGCCCAATATTTTCCCGGGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCCCCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCa                                                                                                                                                                                                     <  1:203950/223‑1 (MQ=255)
                           gcgcGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCg                                                                                                                                                                                                  >  2:844933/1‑239 (MQ=255)
                            cgcgGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGAAAATCCCGCCATAGTAAGCAGCCATCCCTAATTCATCgg                                                                                                                                                                                                 >  2:880498/1‑239 (MQ=255)
                             gcgGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCGGAAAGATAATCCCGCCATAGTAAGCAGGAATCCCTAATTCATCgga                                                                                                                                                                                                >  2:401717/1‑238 (MQ=255)
                             gcgGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGt                                                                                                                                                                                                >  2:679296/1‑239 (MQ=255)
                                    cAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAAt                                                                                                                                                                                         >  2:701408/1‑239 (MQ=255)
                                        tcgttttttCAACGGCAAACAAAGAGGTGGTGGAAAAAAATGGGATGTTGAAAAAAACCGCCCCGGAAACAGTGCCATATAAAAAAAGGAAACGAATTGTGCCTTTAGGCACAATTTTTTACCGCAGGCCCAGAAAAAATCCGGTGAGTATACCGGTAAGCGGATAAAAAAACTTCACCACAAGAAAGAAAAACCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTg                                                                                                                                                                                      <  1:844933/234‑1 (MQ=255)
                                         tccttttaccccGGCAAAAAGGGGGTGGTGCCTATAGACTGACGCGTGGAAATTGGCCTCGGCAGACCCGTGGCATAAAAAATAAAGAGACGTAATGTGCGTTTAGGCCCAATTTTATTCCGCGAGTCGATTAAAATTCGTGTGAGCACTTCGATAAAGGAGGTAAAAACCTCCACCAGAGCAAATATAAACCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgag                                                                                                                                                                                    <  1:401717/229‑1 (MQ=255)
                                           cgtTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCACTTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGGGTCGAGAAAAAATCCGGGGAGCATAACGGCAACCGCGGTAAAAAACTTCCCCAACAGAAAGCTAATCCCGCCATTTAAACAGCCAACCCCAAATTCATCGGAAAAAAATTTgaggg                                                                                                                                                                                  >  1:861753/1‑238 (MQ=255)
                                                   cccgaaaacaaaAGGGTGGGGGGAAAAACTTGGACCTTTAAAATTAACGCCGCCGGACCCGTGAAATAAAAAAAAAAGGGACAGAATTTGCTTTTAGGCCAAATATTTTTCGGGGAGCAAATAAAAAATCGGGGGAGTATATCGGAAAACGCGGAAAAAATCTCCGCCACAAGAAAGATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACag                                                                                                                                                                           <  1:126601/234‑1 (MQ=255)
                                                   ccGGGAAAAAAAAGGGGGTGGGGAAAAAAAGGGGTGGTGCAATTTACCGCGGCCGGAACCTATGTATAAAAAAAAAAGGGAAAAATTTTGCCTTTAGCACAAAATTTTTCCCGGGAGTCGGTAAAAGACCCGGTGAGCATTGTGAAAAACGGGGAAAAAAATCTCGCCACCAGAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACag                                                                                                                                                                           <  1:880498/239‑1 (MQ=255)
                                                       caaacaaaAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTCTAAAATTTCAGGAGATCCACAGTa                                                                                                                                                                       >  2:346972/1‑238 (MQ=255)
                                                                     gtggCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGAACCGATGCAGAACAt                                                                                                                                                         >  1:161833/1‑239 (MQ=255)
                                                                                    gAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCCTCGGCATAAAATTTGAGGAAATACCAAGGACCGAGTCAAAGAATTAAAATCAAAcag                                                                                                                                            >  1:526505/1‑236 (MQ=255)
                                                                                        gtgtaaaTTAACGCCGCGAGACCCGATGAATAAAAAAAAAAGGGACAAAATTGCCTTTAAGGCCAAATTTTTTTCCGCGAGGCGATAAAAAATCCGGTGAGCATTTCGGTAAACGCGGTAAAAAACTCCGCCACAAAAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGt                                                                                                                                        <  2:396458/233‑1 (MQ=255)
                                                                                        ctttgaataaagccccgcggGACCCGTCGGATAAAAAAAAAAGGGAAGAATTTGCCTTATAGGCCAAATAGTTGCCCGGAAGAGGAGTAAAACTCGGGGGGTAATTCGGTTAAACGGGAAAAAAAATTTCGCCACCAGAAAGAAAAGCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACg                                                                                                                                         <  1:701408/224‑1 (MQ=255)
                                                                                         tttaaatttacccccgcggGACCCGCTGAATATAAAAAAAAGGGACGAATTTTTCCGTTAGCCCAAATTTTTTCCCGCGTGTCGAGAAAAAAGCCGGTGAGAATATCGGTAACCGCGAAAAAAAACTCCGCCACCAGAAATATACTCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGt                                                                                                                                        <  1:679296/225‑1 (MQ=255)
                                                                                         ttGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACTCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGAATAAAATTTGCGGAGATACAGAGAACCGATGCAGAGCATTAAATTCGAAACGACGTc                                                                                                                                       >  1:474700/1‑238 (MQ=255)
                                                                                         ttGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGCAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCTTAGTAAGCAGGCATCCCTAATTCCTCGGTATAAAATTTGAGTAGCTACAGAGTCCCGCTCCAGAGCATTAAATTAAAACCAACggg                                                                                                                                       >  1:781368/1‑236 (MQ=255)
                                                                                           gCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAAGGCATTAAATTCGAACCGACGTAGc                                                                                                                                     >  2:253255/1‑238 (MQ=255)
                                                                                                  aaCGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGGGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATAAAAAGTACCAAGGCAAAGAATTAAATTCAAACCGACGGGGCccaaacc                                                                                                                              >  1:396458/1‑239 (MQ=255)
                                                                                                                         aaaaaaaaaaGGGAAAAATTTCGCCTTTAGCCAAAATTTTTCCCCGGGAGTCAAAAAAAAACCCGGTGAGCTTATCGTAAACCGCGAAAAAAACCTTCGCCACCAAAAAGATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAGCCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGa                                                                                                        <  2:18276/237‑1 (MQ=255)
                                                                                                                           aaaatgaaGTGACAAATTTTGTATGTAGCCCATATTTGTTGCCGAGAGTCGAGTAAAACTGCGGTGGAAATTAAGATACACGCGATAAAAAGAGTCGCAACCAAAAAGATATACCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGAtt                                                                                                      <  1:346972/239‑1 (MQ=255)
                                                                                                                           aaaataaaGGGACAAAATTTGCTTTTAGCCCCAATTTTTTTCCGGGAGTCGAGAAAAAACCCGGTGAGCATATCGGAAAACGGGGAAAAAACCTTCCCCCCCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGAtt                                                                                                      <  2:790901/239‑1 (MQ=255)
                                                                                                                           aaaaaaagGGGACAAATTGTTCCTTTGGCCTAAATTTTTTACCGAGAGGTGAAAAAAAATCCGGGGTGTAAAACGGTAAACGGGGAAAAAACCTCCGCACCAAGAAAGTAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGAtt                                                                                                      <  1:144491/239‑1 (MQ=255)
                                                                                                                            aaaaaaaGGGAAATAATTTGTTTTTGGGCCCAAATATTTTCCAGAAGTCAGGAAAAAAACCGGTGAGTAACACGGAAAAAGTGGGAAAAAAACTGCGCCACAAAAAAAAAAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGAtt                                                                                                      <  1:503544/239‑1 (MQ=255)
                                                                                                                                aaaGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGTCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAAAGTACCGAt                                                                                                                                                                  >  2:510331/1‑174 (MQ=255)
                                                                                                                                aaaGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCg                                                                                                                                                                                                  >  2:956731/1‑142 (MQ=255)
                                                                                                                                aaaGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAGCCGACGTCCCCCATACCATAGGGGATTTTTTCCCGCAAAATAAGtt                                                                                                 >  1:18276/1‑239 (MQ=255)
                                                                                                                                  aGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATaaaa                                                                                                                                                                                          >  2:411182/1‑148 (MQ=255)
                                                                                                                                      aCGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAAAGCATTAAATTCGAACCCACGTCGCCCATACCCTAGGCGATTTTTTCCCGCAGATTCCTTTTTAAt                                                                                            >  1:69020/1‑238 (MQ=255)
                                                                                                                                       cGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCccata                                                                                                                                >  2:342531/1‑201 (MQ=255)
                                                                                                                                       cGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGACCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAAATTCAGTTTTaaatt                                                                                          >  1:329211/1‑236 (MQ=255)
                                                                                                                                       cGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCAGCCATAGTAAGCAGGCATCCCTAATTCATCGGGATAAAATTTGACGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGGCGCCCATACCAAAGGCGATTTTTTCCCGCACATTCAGTTTTaaag                                                                                           >  1:828655/1‑236 (MQ=255)
                                                                                                                                         aaaTTTGCCTTTAGGCCAAATTTTTTCCGGCAGATAGAAAAAAACCCCGGTGAGCATTTCGGAAAACGCGATAAAAACCTTCGCCACCAAAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCa                                                                                        <  2:968819/239‑1 (MQ=255)
                                                                                                                                           tttttCCTTGAGGCCCAAATTTTTTCCGCGGGTCGGATAAAAATCCGGGGAGCATATCGGAAAACGCGGTAAAAACCTTCGCCACAAAAAAGATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCa                                                                                        <  2:728637/236‑1 (MQ=255)
                                                                                                                                                       aGGCCAAATTTTTATCCGCGAGGCGAGAAAAAATCCGGGGACAATTGCGGAAAAGGGGGAAAAAACCTGCGCCAGCAGAAAGATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGt                                                                            <  2:526505/239‑1 (MQ=255)
                                                                                                                                                        tgCCCAATTTTTTTCCCGGTGTTGCGAATAAATCCCGTTGACCATATCGGTACACGGTAAAAAAACCTTCGCCACAAAAAAGAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGt                                                                            <  2:151944/238‑1 (MQ=255)
                                                                                                                                                          cccAATATTTTGCCGGGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACAAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCa                                                                          <  2:329211/238‑1 (MQ=255)
                                                                                                                                                             aaTATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAACCTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTCTAAAATTTGAGGAGATACAGAGTACCGATGAATAGCATTACATTCGAACCGACGCCGCCCCTACCATAGGCGATTTTTTCCCCAACATTCAGTTTTATCTGCAGGCGATTATGGtacaaac                                                                      >  1:168383/1‑233 (MQ=255)
                                                                                                                                                                    ttcccGCGAGTCGAGTAAAACCGCGGTGAGAATAGCGAAAACCGCGGAAAAAACCTCCGCCACCAAAAAGATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCa                                                                <  2:933724/236‑1 (MQ=255)
                                                                                                                                                                    ttCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCCCAGTTTCAGTTTTAATTTCAGGGGATTATGGTCaaaataaatca                                                                >  1:54684/1‑228 (MQ=255)
                                                                                                                                                                        cccgtgggtcgtataaaaTCCGGGGGAGTATATCGGTAAGCTCAGAAAACAGCTTCCCAATAAAAAAAATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATTCCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACt                                                              <  1:482376/225‑1 (MQ=255)
                                                                                                                                                                          cgagtcgagtAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGCTTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCtt                                                          >  2:852460/1‑239 (MQ=255)
                                                                                                                                                                          cgagtcgagtAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAAAGAACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAAATTCCGTGGGTTATGGTCAGAAAAAAACACCCCtt                                                          >  1:933724/1‑239 (MQ=255)
                                                                                                                                                                                              cGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAAt                                                                                            >  2:338167/1‑188 (MQ=255)
                                                                                                                                                                                              cGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGCTTATGGTCAGACATAATCACTCCTTTTAACGCCCCGCAGGGc                                         >  2:147277/1‑239 (MQ=255)
                                                                                                                                                                                                ggtgccaataaCGGTATCCGCGGTAAAAACCTGCCCCCCAGAAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGATTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGcg                                        <  2:206812/232‑1 (MQ=255)
                                                                                                                                                                                                 gtagagatatCGGAAAACGGGGTAAAAAACGTCGCCCAGAAAAAAATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATGAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGcg                                        <  2:861753/232‑1 (MQ=255)
                                                                                                                                                                                                    gggagattaggagtaaccgggaaaaaaaaCTTCGACAACAAAAAATAAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGGCCCGCAGGGcg                                        <  2:781368/215‑1 (MQ=255)
                                                                                                                                                                                                      cATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATAAGTCCTTTTAAAGCCCCGCAGGACTCCGttt                                  >  2:130714/1‑239 (MQ=255)
                                                                                                                                                                                                        acgcGGTAAACCGGGAAAAAACTTCCCCAACAAAAAAGAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTttat                                <  2:474700/236‑1 (MQ=255)
                                                                                                                                                                                                         tacGGGAAACGGCGGAAAAAACCTCCGCCACAAGAAAAAAAATCCCGCCATAGTAAGCAGGCATCCCTAATTCAGCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTttat                                <  2:69020/237‑1 (MQ=255)
                                                                                                                                                                                                           acagggaaagggaagaaaaaaaCTTGCCCCCAAAAAAATAACGCCCGTCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTttat                                <  1:675078/223‑1 (MQ=255)
                                                                                                                                                                                                            taatggaaaacgggTAAAAACGCTTCGCCAAAAGAAAGAGAATCCCGCCAAAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTttat                                <  2:81499/227‑1 (MQ=255)
                                                                                                                                                                                                               tAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACACCTTATAACGCCCCGCAGGGCGTCGTtttttattat                          >  1:657078/1‑239 (MQ=255)
                                                                                                                                                                                                                 aaCGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGTTTAAGTTTTAATGTCAGTGGATAATGGTCAGACTTAATCACTCCTTTTACGGCCCCCCAGGGCGCCGTttttaattataa                        >  1:81499/1‑237 (MQ=255)
                                                                                                                                                                                                                         gTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCGt                  >  2:138760/1‑239 (MQ=255)
                                                                                                                                                                                                                          tAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACg                                                    >  2:654522/1‑204 (MQ=255)
                                                                                                                                                                                                                                    cctagacccaaaaaaGAAAACCCCGCCATAGTAAGCAGGCATCCATAATTCATCGGTATAAAATTTGAGGAGATACAGAGTAACGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCGTGCGTTTAc          <  2:871353/227‑1 (MQ=255)
                                                                                                                                                                                                                                     cGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCGTGCGTTTACggg       >  2:920992/1‑239 (MQ=255)
                                                                                                                                                                                                                                            aaaaaaaTATACTCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTAAAAAATTTGAGGAGATACAGAGGACCGATGCAGAACATTAAAATCGAACCCACTGCCCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCGTGCGTTTACGGGCTTCt  <  1:617983/235‑1 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                       
TGTATGAAAGTCCAAACATCATAAACAGCGCGGTGGCAATCGTCGTTTTAACCGGCAAACAAAAGGTGGTGGCGATAAACTGGAGCGTTGCAATTAACGCCGCCGGAACCGCTGCATATAAAATAAAGGGACGAAATTTGCCTTTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGAGCATTAAATTCGAACCGACGTCGCCCATACCATAGGCGATTTTTTCCCGCAGATTCAGTTTTAATGTCAGTGGATTATGGTCAGACATAATCACTCCTTATAACGCCCCGCAGGGCGTCGTTTATTATTATTACGCCGTGCGTTTACGGGCTTCT  >  NC_000913/4065375‑4065832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: