Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,241,837 G→C 11.0% G139G (GGC→GGG xylE ← D‑xylose transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,241,8370GC11.0% 194.6 / 4.0 79G139G (GGC→GGGxylED‑xylose transporter
Reads supporting (aligned to +/- strand):  ref base G (51/17);  new base C (2/7);  total (53/26)
Fisher's exact test for biased strand distribution p-value = 3.21e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

CATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTG  >  NC_000913/4241609‑4242068
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                         
caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATAcct                                                                                                                                                                                                                                >  2:871469/1‑237 (MQ=255)
caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCg                                                                                                                                                                                                                                >  2:898055/1‑238 (MQ=255)
caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGc                                                                                                                                                                                                                               >  2:789203/1‑239 (MQ=255)
caTTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATAAGCTGGAGCCAGTTCCGCAAAATACATTGGCGCAAGCATTGAGGCTAAACCAACCCCAAAAcccc                                                                                                                                                                                                                               >  1:763616/1‑237 (MQ=255)
caTTAAGAACAGCAGTACTGGGATACATTCCGAGGCAAACATATAACCCCAGCCGTCAGTATTCACCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTCAAAGTTGCCCGCAAATAATCACAAACTGGTTAATAGAGACCAGGTTCCCGCGACATGGCCCTGGGACCACGTACGCAATATACATTGGCGAAAGCATTGAGCGTAAACCAAAGCCAATaaccc                                                                                                                                                                                                                                >  1:819496/1‑235 (MQ=255)
  ttAAGAACAGCCATGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGACATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAATTGCCCGAAACTAATCGCAAACTGGTTAAAAGAGACCACTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATATGCGGGACCATTGAGACTAAACCAACGcacaacccgccc                                                                                                                                                                                                                               >  2:631347/1‑228 (MQ=255)
          agcagTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGTCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCACTAATGCg                                                                                                                                                                                                                     >  2:305501/1‑239 (MQ=255)
          agcagTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATGGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTACGCAATATACATTGGCGAGAGCATTTAGGCTAAACCAACGCCAATACCGCCAATAATGCg                                                                                                                                                                                                                     >  2:433022/1‑239 (MQ=255)
          agcagTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCACTACCGCCATAAATGCg                                                                                                                                                                                                                     >  2:744909/1‑239 (MQ=255)
               tGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGataaa                                                                                                                                                                                                                >  2:838790/1‑239 (MQ=255)
                 cAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAACCTGGTTAAACGAGACCAGTTTCCCGCGAATATGAGCTGGGGCAGTTTCCTCCATATACCTGGGCGCGCGCCATCAGGACAAACCAAACCCAAACCAGCCCACAAAGCGaaaaaaa                                                                                                                                                                                                              >  1:343208/1‑237 (MQ=255)
                 cAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTAAGGCTAAACCAACGCCAATACCGCCAATAATCCGataaata                                                                                                                                                                                                              >  2:658511/1‑239 (MQ=255)
                   taggagttgtaaaaaacacaaataaAACGCCCGCCGTAAGTATCCAGCCAGGTGGCACACCCGGAACGGGAAAAAAAAGATTTTCGGAATAAAACAAAAAGTTGCCCGAAAATAACCGAAAACGGGTAAAAAGAGACAAGTGCCCCGGGAATATGAGCTGGAGCCAGTTCCGCAATATACATGGGCGAGAGAATTGAGGCTAACCCAACCCCAATACCGCCAATAATGCGATAAATAAc                                                                                                                                                                                                            <  1:747139/218‑1 (MQ=255)
                              cgcgcaaaaCATATAACGCATGCTTTTAGTATCCCTCCTGTTGGAACCCCCGCATCGGCAAAAAAAATAGTGTACGGAGTAAATTAAAGTTTGCAGAAAAATCACCGCAACCGGGTCAAAAGGAACCAGTCCCCCGCAAATAGGAGCGGGAGCTCCCCCCCAACAACCACTGGGGGAGAGAATGGAGGCAAACCAACCCCAAAGACCGCCAATAATGCGATAAATAACAAATTCCGGGa                                                                                                                                                                                                 <  1:477074/233‑1 (MQ=255)
                              cGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTCAAAGATGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCAGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCCAAAAGGCGATAAAAAACAAAAACCGGGa                                                                                                                                                                                                 >  1:367874/1‑239 (MQ=255)
                               gAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGGGCCAGTTCCGCAATATACATTGGCGAGAGCATTTAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAAAAACAAAATCCGGGAc                                                                                                                                                                                                >  1:253976/1‑239 (MQ=255)
                               gAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCAAATACAGCCAATAATGCGATAAATAACAAATTCCgggcc                                                                                                                                                                                                >  2:342672/1‑237 (MQ=255)
                                        ttagaacgccgcgcgacAGTAATCAGCCATCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTGGCCCGAAAATAATCGCAAACTGGTTAAAAGAGCCCAGTTCCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATGGGCGAGAGCATTGAGGCTAAACCAACGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGc                                                                                                                                                                                       <  2:786653/223‑1 (MQ=255)
                                        ttaaaaCGCCAGCCGTAAGTCTTCAGCCAGCCGGCCTCACCGGAACGGGAAATAAAATAGTTTACGAAGTAAACTAAAAGTTGCAAGAAAATAATCGAAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGc                                                                                                                                                                                       <  2:977888/235‑1 (MQ=255)
                                         ttaataagagacagTCAGTATTCAGCCAGCTGCCATCACGGGAACGGGAAATAAAATAGTTTACGCAGAAAACTAAAAGTTGCCCAAAAATAATCGCAAACTGGTTAAAAGAGACCATTTCCCCGCGAATATGAGCTGGAGCAATTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGcc                                                                                                                                                                                      <  2:279410/226‑1 (MQ=255)
                                            acccccAGCCTTCAGTCTTCAGCCAGCTGGACACAGTGAAACGTGCAAAAAAATATGTTACAAAGTAAACAAAAAGTGGCCCAAAAAAAACGGCAAACGGGTTAAAAAAGACCAGTTCCCCGGGAATATGGGCTGGGGCCAGTCCCGCAATATACATTGGGGAGAGCATTGAGGATAAACCAACCCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGa                                                                                                                                                                                   <  1:578109/235‑1 (MQ=255)
                                              cccaaGCCTTCAGTTTCCGGCCAGCGGGCATCCCCGGAACGGCAAAAAAAAATGTTAACGCAGTAACCTAAAGGTGGCCGAAAAATAACCGAAAACTGGTAAAAAGAGCCCAGTTCCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGGGAGAGCATTGAGGCTAACCCAACCCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATa                                                                                                                                                                                 <  2:253976/235‑1 (MQ=255)
                                                 gacgggtgaGTTTTGAGCCGGCTGGCATCCCGGAAAAGGCAAAAAAAAGATTTTACGCAGAAAACAAAAGAGTGACAAAAAAAAAGCGAAAACGGTTGAAAAGAGACCAGTTCCGCGCAAGAATGAGCGGGGGCCAGTCCCCCAATAGACTTTGGAGAAAACATTGAGTAAAAACAACCCCAAATCCCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATaaa                                                                                                                                                                               <  1:479460/230‑1 (MQ=255)
                                                 cggCCGTCAGTATTCACCCAGCTGGCAGCCAGGAAACGGGAAATAAAATAGTTTACGCAGTAAACTAAAAGTGGCCCAAAAATAATCGCAAACTGGTAAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAAc                                                                                                                                                                              <  2:614671/237‑1 (MQ=255)
                                                 cgcccataagtttccagccggcTGCCAACCCCGGAACGGACAAAAAAATAGTTTCCGAAGAAAACAAAAAGTGGCCCGAAAAGAATCGAAAACTGGTTAAAAGAGACCAGTTCCCCGGAAATAGGGGCTGAAGCAAGTCCCGAAATATACATGGGCGAGAGCATTGAGGCAAAACCAACCCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAAc                                                                                                                                                                              <  2:150276/225‑1 (MQ=255)
                                                 cacccgtcttttttCAGCCGGTTGGAATCCCCGGAAGGGAAAAAAAAATTGTTTACACAGAAAATAAAAAGTGGCCGAAAAAAAACGCAAAACGGGTAAAAAGAGACAGGTTCCCCGCAAATTTGGGCTGGAGCCAGTTCCGCAATATACATTGGAGAGAGCATTGAGGCTAAACCAACCCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAAc                                                                                                                                                                              <  1:366322/227‑1 (MQ=255)
                                                 cAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATaaaa                                                                                                                                                                              >  2:850387/1‑238 (MQ=255)
                                                  aGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCACTACCGCCAATAATGCGATAAATAACAAATTCCGGGCACTAACCTGCCCGCTAAACa                                                                                                                                                                             >  2:344570/1‑239 (MQ=255)
                                                  aGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATCATGCGATAAAAAACAAATTCCGGGACATAACCTGCCAGAAAAACa                                                                                                                                                                             >  2:698732/1‑239 (MQ=255)
                                                  aGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAACCa                                                                                                                                                                             >  2:1005797/1‑239 (MQ=255)
                                                   gCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCggg                                                                                                                                                                                                  >  2:747139/1‑217 (MQ=255)
                                                   gCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATCCCGGAACACAACCTGCCAGATAAACaa                                                                                                                                                                            >  1:634127/1‑238 (MQ=255)
                                                      gtaaGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCAGAAAATAATCGAAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGca                                                                                                                                                                         <  2:763616/236‑1 (MQ=255)
                                                      gTCAGTATTCAGCCAGCTGGCATCCCCGGAACGGGAAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCACAAAAATAATCGCAAACTGGTTAAAAGAGACAAGTTTCCCGCGAATAGGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGca                                                                                                                                                                         <  2:496589/239‑1 (MQ=255)
                                                        cAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTTAGGCTAAACCAACGCCAATACCGCCAATATAGCGATAAATAACAAATTCCGGGCAATAACCTCCCAGAAAAAAAGGcaca                                                                                                                                                                       >  2:479460/1‑239 (MQ=255)
                                                           tttcccaccggttGGCATCCCCGTACGGTGAAAAAAATAGTTTTCCCGATTAAATGAAAGTGTGCCGGAAAATACTGAGAACCTGGTTAAAAGAGTCATGTGCCCCGCGAATATTGGGTGGACCCTGTTCCAAAATATACATTGGCGAAGGCATTGGGGCTAAACCAGCGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAgtg                                                                                                                                                                    <  1:305501/227‑1 (MQ=255)
                                                                cccgatgttgccacacaGGAAGGGGAAAAAAAATATTTTCCGCATTAAAATAAAAGTTGCGAAAAAATATGCCAAAAGTGGTAAAAGGAGCCCATTTTCCCGCGATTATGGGCTGGAGCCAGTTCCGAAATATACTTTGGGGAGAGCATTGAGGCTAAACCACCGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTc                                                                                                                                                               <  1:850387/226‑1 (MQ=255)
                                                                acccagtgtcatgccccGAAAAGGGAAATAAAATAGTTTACGAAGAAAAAAAAAAGTTCCCGAAAAATAATCGCAAACGTGTAAAAAGAGCTCTGTTCCCGACAAATATGAGCGGGAGCATGTGCCACAATATCCATTGGGGAGAGTATTGAGGCTAAACCACCGCAAATTCCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTc                                                                                                                                                               <  1:698732/224‑1 (MQ=255)
                                                                  ccagcTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTAAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACCAAACCTGCCAaaaaaaaaagctggccctaaaaaa                                                                                                                                                             >  1:279410/1‑221 (MQ=255)
                                                                  ccagcTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGACCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAAAACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTCCCAGATAAACAGGCAAAACGTTGTCCg                                                                                                                                                             >  2:561270/1‑239 (MQ=255)
                                                                     gcTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGAtctgtcccttttacacatccccga                                                                                                                                                           >  2:894803/1‑215 (MQ=255)
                                                                     gcTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGGGTTGTCCGGGt                                                                                                                                                          >  2:356407/1‑239 (MQ=255)
                                                                           caatcccgggacacgaaaTAAAATTATTTAAGCAAAAAAATAAAAGTTTCCCAGAAAAAAACAGCAAATGGTTTAAAAAGACCAGTTTCCCGCGAAATTGGTGCGGGACCCAGTCCCAAATAATAAATGGGCGAGAGAATGGAGTTTAACCAAACGACAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTtat                                                                                                                                                      <  1:615833/223‑1 (MQ=255)
                                                                                        gCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGCATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAAAGGTAAAACCAAg                                                                                                                                       >  2:240227/1‑239 (MQ=255)
                                                                                                     tttACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGGGTTGCCCGGGTTTATAAAGGAAAAACCAAGTACTGGCCAGGc                                                                                                                           >  1:355911/1‑239 (MQ=255)
                                                                                                      ttccacagtaacaaaaaaGTTCCCAAAAAATATTCGAAAATGGTTAAAAAAGGACAGTTTCCCCGGAAAATAGCGCGGGGCCAGGTTCCACAATATCCATGGGCGAAATCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCa                                                                                                                          <  1:356407/225‑1 (MQ=255)
                                                                                                       tACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCCGATAAACAGGCACAGTGTTGTCCGGGTTTATAAGGGAAAAACCAAATTTTGGCCAgagag                                                                                                                         >  1:869511/1‑235 (MQ=255)
                                                                                                                      aaGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCATAACCTACACCAGaaa                                                                                                          >  2:427687/1‑239 (MQ=255)
                                                                                                                        gTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGGAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATa                                                                                                        >  2:150525/1‑239 (MQ=255)
                                                                                                                          tttcccgaaaaaaaACCGCAAACTGGTAAAAAGAACCCATTTCCCCGAAAATATGAGCGGGACCCAGTCCCGAAATATACATGGGCGAGAGCATTGAGGCTAAACCAACGCAAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATa                                                                                                        <  2:869511/231‑1 (MQ=255)
                                                                                                                             cccGAAAATAATCGCAAACTGGTTAACAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACCTTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGCTAAACAGGAACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTTTGGCAAGGCGACACCACCCCCGaaaaaaaaaa                                                                                                   >  1:715431/1‑239 (MQ=255)
                                                                                                                             cccGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCAAGGCAGAACCTACACCAGAAATacaaa                                                                                                    >  2:133946/1‑239 (MQ=255)
                                                                                                                                      agaaacgaaaaCTGGTAAAAAGGCACCATTTCCCGGAGATTATGAGGTGGAGACAGTCACGAAACAACGTTGGGGCAGAGCATGAAGCTAAAACCAGCACAATTCACGACAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAgggc                                                                                             <  1:744909/231‑3 (MQ=255)
                                                                                                                                          cccAAACTGTTTAAAAGGGACCTTTGCCCGGCGAATTGGGCCGGGAGCCATTTCCGCAATAGCAATGGCCGAGACAATGGAGGCAAACCCAACGCAAAACCCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGAcagcag                                                                                        <  1:427687/237‑1 (MQ=255)
                                                                                                                                          ccagaatgtggtaaaaaggacacAGTTCCCCGAGAATTGGGGGTGGGCCAAGTTCCGAAAAATCCATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGAcagca                                                                                         <  2:367874/217‑1 (MQ=255)
                                                                                                                                                    gTTAAAAAAGCCCAGTTCCCCGAAATTATAGCAGGGGGCAAGTCCACAAAAAACCATTGGGGAGAGAGTTGAGACAAACCAACACCCATACCCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTaa                                                                               <  1:342672/239‑1 (MQ=255)
                                                                                                                                                              aCCAGTTTCCCGCGAATCTGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTc                                                                                                                                                               >  1:11101/1‑149 (MQ=255)
                                                                                                                                                              aCCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCacg                                                                     >  2:438750/1‑239 (MQ=255)
                                                                                                                                                              aCCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCacg                                                                     >  2:699577/1‑239 (MQ=255)
                                                                                                                                                              aCCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCac                                                                      >  2:410387/1‑238 (MQ=255)
                                                                                                                                                              aCCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGAATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTc                                                                                                                                                               <  2:11101/149‑1 (MQ=255)
                                                                                                                                                                  gTTCCCCGCGAATATGGGCTGGAGCCAGTTCCGAAATTTAAATTGGCGAGAGAATTGAGGCTAAACCAACGCCAATTCCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCAcgacga                                                                 <  2:355911/239‑1 (MQ=255)
                                                                                                                                                                       ccGCGAATAGGAGCTGACGCAAGTCCCGCAATACACATGGGCGATAGCATTGAGGCTAAACCACCGACAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGaa                                                            <  2:819496/239‑1 (MQ=255)
                                                                                                                                                                        cgcgAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTCCACCAGAAATAAAAACAAGGACAGCAGCAATCTTAAAGAAAACACCACGACCaaaa                                                           >  1:430856/1‑238 (MQ=255)
                                                                                                                                                                          cgAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCgg                                                                                                                                                            >  2:274340/1‑140 (MQ=255)
                                                                                                                                                                                              aGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCTGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGCCGACCGAAGCGGTTACTGCAATAaccacc                                       >  2:132819/1‑239 (MQ=255)
                                                                                                                                                                                                               aTTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTCCACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGAAAAAACCACCGAGGGCACCCCCcat                        >  1:235021/1‑239 (MQ=255)
                                                                                                                                                                                                                  tGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCgatga                      >  2:129737/1‑239 (MQ=255)
                                                                                                                                                                                                                  tGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCAAGGGCACCGCCgttga                      >  2:240523/1‑239 (MQ=255)
                                                                                                                                                                                                                   ggCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACCCCAGAAATAAAAAACAGGACAGCAGAAATCTTAAGTGAATACCAACGACCGAAACGGTACATGCAATAACCACCAAGGGACCCGCCgaa                        >  1:638587/1‑235 (MQ=255)
                                                                                                                                                                                                                     cGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTtata                                                                                                                                                     >  2:337662/1‑109 (MQ=255)
                                                                                                                                                                                                                     cGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGc                   >  2:285941/1‑239 (MQ=255)
                                                                                                                                                                                                                        agagCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGGGAATCACGACGACCGAAACGGTTACTGCAATAACAACCGAGGGCACCGCCGATGATGCa                  >  2:612601/1‑238 (MQ=255)
                                                                                                                                                                                                                          agCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGGTGATGCAAcc               >  2:735725/1‑239 (MQ=255)
                                                                                                                                                                                                                          agCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGAACCGCCGATGATGCAAcc               >  2:927085/1‑239 (MQ=255)
                                                                                                                                                                                                                               tGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGa                                                                                              >  2:98050/1‑155 (MQ=255)
                                                                                                                                                                                                                                        aaCCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCaacacgctg  >  2:651575/1‑231 (MQ=255)
                                                                                                                                                                                                                                              cGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCa                                                                                                              >  2:361322/1‑125 (MQ=255)
                                                                                                                                                                                                                                              cGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCa          >  2:830215/1‑225 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                         
CATTAAGAACAGCAGTGCAGGGATACATTCCGAGGCAAACATATAACGCCAGCCGTCAGTATTCAGCCAGCTGGCATCACCGGAACGGGCAATAAAATAGTTTACGCAGTAAACTAAAAGTTGCCCGAAAATAATCGCAAACTGGTTAAAAGAGACCAGTTTCCCGCGAATATGAGCTGGAGCCAGTTCCGCAATATACATTGGCGAGAGCATTGAGGCTAAACCAACGCCAATACCGCCAATAATGCGATAAATAACAAATTCCGGGACATAACCTGCCAGATAAACAGGCACAGTGTTGTCCGGGTTTATAGAGGTAAAACCAAGTTCTGGCCAGGCAGAACCTACACCAGAAATAAAAAACAGGACAGCAGCAATCTTAAGTGAATCACGACGACCGAAGCGGTTACTGCAATAACCACCGAGGGCACCGCCGATGATGCAACCAATCAGAGCGCTG  >  NC_000913/4241609‑4242068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: