BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	422,725	Δ1 bp	3.1%	coding (208/1815 nt)	malZ →	maltodextrin glucosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	422,725	0	C	.	3.1%	372.5 / 2.4	127	coding (208/1815 nt)	malZ	maltodextrin glucosidase
Reads supporting (aligned to +/- strand): ref base C (76/44); new base . (2/2); total (80/47)
Fisher's exact test for biased strand distribution p-value = 6.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01

Only 100 of 132 total aligned reads displayed.

ATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGCCGTCGATGTACCG‑GATATCGGCCCACAATGGG‑CTGCGGATC‑AGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGATCATGTC  >  NC_000913/422490‑422955

 tcatttttttaaccagggggAATTATGTATTTAATTGCATGGCCCCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAACATCAACTGCTCATTACCCTGGGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGGACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGAtctctc                                                                                                                                                                                                                                           <  1:1040305/223‑1 (MQ=255)

      ttttactcaCAGGGTAATTATGAGGTTAAAGGCCTGGCCCTTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑a                                                                                                                                                                                                                                        <  1:17150/230‑1 (MQ=255)

       tttCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGGACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCgg                                                                                                                                                                                                                                   >  2:1040305/1‑239 (MQ=255)

       tttCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTCCGTACAGAACACGAGAACGAAGAAATGTCACTACCAAACCATAAGCAACGCAGTCACCGGAAGCCTGGCGCAACCGCCGGGCGAGCGGGCATAGATCTCCCC‑A‑CCgg                                                                                                                                                                                                                                   >  1:693448/1‑239 (MQ=255)

                         tttattttAAATGCAAGGCCCCTGGCGGTGCCCCCTTTTTTTAAACAAAGCAAAGAACCACCGCTCATTACACTGGGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGACCACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTTAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTa                                                                                                                                                                                                                 <  1:474511/233‑1 (MQ=255)

                         tgtgtttaaaaTGCATGGCCTCCTCCGGGTCCCCCATTTGTTAAACAAAGCAAAGAACAACTTCTTATTACAATGTGGGTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCCGTCAGCCGCAGCCTGGCGTCCCCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTa                                                                                                                                                                                                                 <  1:1248357/231‑1 (MQ=255)

                          tgatgTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGAtctctc                                                                                                                                                                                                                                           >  2:335774/1‑214 (MQ=255)

                            atgTTAAATGCATGGCCCCTGCCGGTGCCTCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGCGGCCGCCGTGCGAAGACCCACCGCAGCGCATTATGCTGCTTACAGAACAGGATAACGAGCAAATGCCAGTACCGATGAATACTCACCGCAGTCAGCCGCAGCTCGGCGTCTCTGCATGGCGCTCGTCGATTCATTCCTCC‑G‑CCGGACACACCcgcgtcgcgtaccg                                                                                                                                                                                                              >  1:130147/1‑227 (MQ=255)

                            atgTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAg                                                                                                                                                                                                              >  2:474511/1‑239 (MQ=255)

                              gTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGCTGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCCTGCGCCGATTGATCTCTCC‑A‑GCGGACAACCCCGgcggcggaacatt                                                                                                                                                                                                             >  1:202810/1‑231 (MQ=255)

                                 aaaTGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCa                                                                                                                                                                                                         >  2:268163/1‑239 (MQ=255)

                                  aaTGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACCGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAAACCGATAACGACGAAATGCCAGTCCCGATCCATACGAAGCGCAGGAAGCCGCAGCCTGGGGTACCCCCCAGGCGTGCCGCGATTGAT‑TCACCGC‑GCGGACAACACaggcgccaccacaacttcca                                                                                                                                                                                                        >  1:101696/1‑224 (MQ=255)

                                   agccggtcccctgtgcGGTGCCCCCATTTGGTAAACAAAGCAAAGCACACCTGCTCATTACCCTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCaaa                                                                                                                                                                                                       <  1:1117919/224‑1 (MQ=255)

                                         ggCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAActgctg                                                                                                                                                                                                 >  2:1255797/1‑239 (MQ=255)

                                         ggCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAActgctg                                                                                                                                                                                                 >  2:1123052/1‑239 (MQ=255)

                                         ggCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑CCGGACAACCCCGGCGGCGTTCCAGTTTCAAActgctg                                                                                                                                                                                                 >  2:1255370/1‑239 (MQ=255)

                                                gCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGCGGCTGACGGGCGAAGACCCACCGACACGCATAAAGCTGCGTCCAGAACACGATAACGAAGAAATGACAGTACCGATGCATAAGCAGCGCCGTCACCCGCAGCCTGGCGCACCCGCACGGCGTACGGCGCTGGATCGCACC‑A‑GCGGACACCCCCGCCGGCGTCTCAGtgataaacgcacgtggcacc                                                                                                                                                                                          >  1:1064905/1‑220 (MQ=255)

                                                cctggTGCCACGATTTGTTAAAAAAAGCAAAGTACAACTGCTCATTACACTGTGGCTGACGTACGAAGACCCACCGCACCGCATTATGCTGCGTACAGAAACCGATAACGAAGAAATGTCAGTTCCGATGCATAAGGAGCGCCGTCAGCCGCAGCCTGGCGGCACCGAATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACg                                                                                                                                                                                          <  1:1257785/236‑1 (MQ=255)

                                                         cccATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGCCGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCTTAAGCACCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGGTTCATCC‑A‑CGGGCCAACCCCGGCGGCGGTACCGTTTCAAACGCCCTGGGCACAATCGCCacg                                                                                                                                                                                 >  1:607006/1‑237 (MQ=255)

                                                          ccATTTGTTAAACAAATCAAAGAGCAACTGCTCATTCCACTGGGGCTGACGGGCGAAGACCCACCGCAGGGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCg                                                                                                                                                                                <  2:558419/239‑1 (MQ=255)

                                                               tGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGt                                                                                                                                                                          >  2:26369/1‑239 (MQ=255)

                                                                      caaagcaaagATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTa                                                                                                                                                                                                                 >  1:905173/1‑194 (MQ=255)

                                                                      caaagcaaagATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTa                                                                                                                                                                                                                 <  2:905173/194‑1 (MQ=255)

                                                                        aggcaagtattaaCTGCTGATTACACTTTGGCTGCCGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGc                                                                                                                                                                 <  2:837889/228‑1 (MQ=255)

                                                                         agcaaagATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑CCGGACAACCCCGGCGCGGTTACAGTTTCAAACTGCTGTGGCCCGACCCCCACCGTT‑GGTTTACAcccag                                                                                                                                                                >  1:149514/1‑236 (MQ=255)

                                                                           caaagATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCCCGATCGCCAGCGTT‑GGTTTACACCGCAgg                                                                                                                                                              >  2:1246871/1‑239 (MQ=255)

                                                                           caaagATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGATTCAAACTCCTGTGGCCGGATGGCCAGCGTT‑GGTTTACACCGCAg                                                                                                                                                               >  2:670327/1‑238 (MQ=255)

                                                                                 tCAACGTCTCATTACACGGTGGCGGACGGGCGAAGACCCACCGGAGCGCAATATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGGACCGATGCATAAGGAGCGCAGTCAGCCGCAGCCGGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCtt                                                                                                                                                          <  2:698709/237‑1 (MQ=255)

                                                                                        cTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAAt                                                                                                                                                 >  2:95948/1‑239 (MQ=255)

                                                                                          cATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGCTTGATCTCTCC‑A‑GTGGACAACCCCGGCGGCGTTAACGTTTCAAACTGCCGTGGCACGAGCGCC‑CCGTTCGGTTTACACCGCAGGGCTTCCGCCGAATg                                                                                                                                                >  1:923391/1‑238 (MQ=255)

                                                                                          cATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAACCCGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATg                                                                                                                                                >  2:157710/1‑238 (MQ=255)

                                                                                                  tgtgGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCACCGCAGTCAGCCGCAGCCCGGCGTCACCGCATGGCGTGCGGCGCTTGATCCGTCC‑A‑GCGGCCAACCCCGGCGGCGTTCACGTTTCAAACTGCTTGGGCACGAGCGCCACCGTG‑GGGTTACACCCCAGGGCTTCACCCCAAACCCGCCGG‑c                                                                                                                                       >  1:654823/1‑238 (MQ=255)

                                                                                                   gttgcgggaggggaaaGACCCCACGCAGCGCATTAGGCGGCGTACAGAATACGATAACGAAGAAATGGCAGTACCGAGTGAAAAGCAGCGCAGTACGCCGTAGCCTGGCGTCACCGCATGGCGTGCGACGATTGGTCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CAc                                                                                                                                     <  1:921633/225‑1 (MQ=255)

                                                                                                   ggggCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CAc                                                                                                                                     <  2:106900/237‑1 (MQ=255)

                                                                                                    tgGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CCCg                                                                                                                                    >  2:576148/1‑239 (MQ=255)

                                                                                                     ggCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGGGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGACGCCGG‑CACg                                                                                                                                    >  2:28762/1‑238 (MQ=255)

                                                                                                       cTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCa                                                                                                                                                                                                         >  1:316356/1‑169 (MQ=255)

                                                                                                       cTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCa                                                                                                                                                                                                         <  2:316356/169‑1 (MQ=255)

                                                                                                               cGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACCGTTTCCAACTCCTGTGCCCCGATCGCCAGCCTT‑GGTTTCCACCCCAGGGCTTCAGCACAAAGCCGCCGG‑CCCGACGGGAgagtg                                                                                                                         >  1:300903/1‑235 (MQ=255)

                                                                                                                     cccACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGc                                                                                                                     <  2:654823/237‑1 (MQ=255)

                                                                                                                     cccACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGCCg                                                                                                                   <  2:693448/239‑1 (MQ=255)

                                                                                                                        aCCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CCCGACTGGCGCAGTTTGCCGTCg                                                                                                                >  2:376136/1‑239 (MQ=255)

                                                                                                                        aCCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGccgccg                                                                                                                >  2:794884/1‑239 (MQ=255)

                                                                                                                             gcgtcATTTTGCTGCGTACAGAAACAGATACCGAAGAAATGTCAGCGCCGAGGCATAAGCAGGGCAGTTAGCCGGAGCCGGGCGTCACCGCACGGCGTGCGGCGATTGATCTCTTC‑A‑GCGGACGACCCCGGCGGCGTTACAGTTTCAAGCTGCTGGGGCACGACCGCCAGCGTG‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGCCGTCGATGTa                                                                                                           <  1:1222913/235‑1 (MQ=255)

                                                                                                                               cgcATTATGCTGCGTACAGAACACGATAACGAAGCACTGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑ACGGACAACCCCGGCGGCCTTACAGTTTCAAACTGCTGTGGCCCGATCGCCACCGTT‑GGTTTACAGCGCAGGGATTCACCCCAAGTCCCCCGG‑ACCCACTGGGGGGGTTTTCCgcgcatgaccg                                                                                                         >  1:1219276/1‑229 (MQ=255)

                                                                                                                                gacagagtgtggttACAGAACAAGGAAAAGAAAAAATTTCGGTACCGGGGGATAAGCAGCGCAGTCCGCCGCCGCCGGGCGGACCCGGATGGCGTTGGGCGATGGATCTCTCC‑A‑GCTGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGGCGCCGG‑CACGACTGGAGCAGTTTGCCGTCGATGTAcc                                                                                                         <  1:753674/225‑1 (MQ=255)

                                                                                                                                         tgcGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGCCGTCGATGTACCG‑GATATCGGc                                                                                              <  2:1219276/239‑1 (MQ=255)

                                                                                                                                         ggcGTACAGAACCCGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGCCGTCGATGTACCG‑GATATCGGc                                                                                              <  2:202810/238‑1 (MQ=255)

                                                                                                                                          gcctACAGAACCCGATCACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGCCGTCGATGTACCG‑GATATCGGc                                                                                              <  2:77761/235‑1 (MQ=255)

                                                                                                                                           cGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGGTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CCCGCCTGGAGCAGTTTGCCGTCGATGTCCCG‑GTAATCGGccc                                                                                            >  2:291305/1‑239 (MQ=255)

                                                                                                                                                aGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGCCGGCGATGTACCG‑GATAACGGccccgt                                                                                         >  2:753674/1‑234 (MQ=255)

                                                                                                                                                     acGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGCCGTCGATGTACCG‑GATATCGGCCCACAATgg                                                                                     >  2:847293/1‑236 (MQ=255)

                                                                                                                                                     acGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑ACCGACTGGAGCAGTTTGCCGTCGATTGACCG‑GATATCGGCCCCCAATgg                                                                                     >  2:632921/1‑236 (MQ=255)

                                                                                                                                                     acGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGACGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCCCAGGGCTTCAGCCGAATGCCCCCGC‑CACGACTGGCGCAGTTTTCCGTCTCAGTACCG‑GGAATCGGCCCCCaacagccc                                                                                  >  1:503062/1‑233 (MQ=255)

                                                                                                                                                     acGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCACCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAACCTGCTGTGGCCAGATCCCCAGCGTT‑GGTTTCCACCGCAGGGCTTCACCCGACGGCCGCCGG‑CCCGACTGGAGCCGTTTGCCATGGATGTccccgtaagcgccccccaacgg                                                                                      >  1:976018/1‑217 (MQ=255)

                                                                                                                                                       gATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACACCCCCGGCGGCGTTCCAGTTTCAAACTGCTGTGGGACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTCCAGCCGAACGCCCCCGG‑CACGACTGGCGCCGATTTCCGACGATGACCCC‑GGTAACGGCACCCAAAGGG‑CAGc                                                                               >  1:1218923/1‑239 (MQ=255)

                                                                                                                                                       gATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGCCGTCGATGTACCG‑GATATCGGCCCACAATGGG‑CTGc                                                                               >  2:1189585/1‑239 (MQ=255)

                                                                                                                                                            cGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCcgg                                                                                                                                                                                                                         <  1:1113262/100‑1 (MQ=255)

                                                                                                                                                            cGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCcgg                                                                                                                                                                                                                         >  2:1113262/1‑100 (MQ=255)

                                                                                                                                                            cGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGCCGTCGATGTACCG‑GAAATCGGCCCACAATGGG‑CTGCGGATc                                                                          >  2:1036782/1‑239 (MQ=255)

                                                                                                                                                                   aTGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCCTGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCCCGATCCCTAGCGTT‑GGTTTCCACCCCAGGGTTTCATCCGTATCCCGCCGGACCCCAC‑GGACCAGTTGCCCGGCGAGGTACCG‑GCACCCGGCCCCCAAAGGG‑CGGCGGacagaatgtt                                                                   >  1:605857/1‑230 (MQ=255)

                                                                                                                                                                   aTGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTAAACCGCAGGGCTTCAg                                                                                                                                                       >  2:1092584/1‑158 (MQ=255)

                                                                                                                                                                       cAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGCCGTCGATGTACCG‑GATATCGGCCCACAATGGG‑CTGCGGATC‑AGATTTTTTAt                                                              <  2:923391/239‑1 (MQ=255)

                                                                                                                                                                       cAGTACCGAGGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGCCGTCGATGTACCG‑GATATCGGCCCACAATGGG‑CTGCGGATC‑AGATTTTTTAt                                                              <  2:300903/239‑1 (MQ=255)

                                                                                                                                                                        aggcccGTTGCTTAAGCGGCGCAGTCCGCCGTAGCCGGGCGTCACCGCATGGCGGGCGGCGATTGATCTCGCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTAAAACGGCTGTGGCACGAGCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGCCGTCGATGTACCG‑GATATCGGCCCACAATGGG‑CTGCGGATC‑AGATTTTTTAt                                                              <  1:330230/234‑1 (MQ=255)

                                                                                                                                                                        aGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGCCGTCGATGTACCG‑GAAATCGGCCCCCAATGGG‑CTGCGGTTC‑AGATTTTTTTTc                                                             >  2:409649/1‑239 (MQ=255)

                                                                                                                                                                            ccGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGCCGTCGATGTACCG‑GATATCGGCCCACAATGGG‑CTGCGGATC‑AGATTTTTTATCAGAt                                                         <  2:821348/239‑1 (MQ=255)

                                                                                                                                                                               aTGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGCTCTCTCC‑A‑GCGACCAACGCCGTCGGCGTTACAGTCTCAAACTGCTGTGGCACGACGGCCAGCGTT‑GGTTTACACCGCAGGGCTTTAGCCGAATGCCGCCTG‑CCCGACTGGAGCAGCTTTCCGCTGCTGAACCG‑CGTAGCGACCCACCATGGT‑GTCCGGGCCAAGATTTTTTacatcaat                                                        >  1:465228/1‑231 (MQ=255)

                                                                                                                                                                               aTGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGCCGTCGATGTACCG‑GATATCGGCCCCCAATggg                                                                                    >  2:1244531/1‑211 (MQ=255)

                                                                                                                                                                                    tAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGCCGTCGATGTACCG‑GATATCGGCCCACAATGGG‑CTGCGGATC‑AGATTTTTTATCAGATCttg                                                     >  2:858107/1‑234 (MQ=255)

                                                                                                                                                                                    tAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGCCGTCGATGTACCG‑GATATCGGCCCACAATGGG‑CTGCGGATC‑AGATTTTTTATCAGATCTTCCCTg                                                 >  2:846377/1‑239 (MQ=255)

                                                                                                                                                                                      agccgcGCAGTCAGCCGCAGCCTGGCGTCACCGGATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGCCGTCGATGTACCG‑GATATCGGCCCACAATGGG‑CTGCGGATC‑AGATTTTTTATCAGATCTTCCCTGAt                                               <  2:797037/239‑1 (MQ=255)

                                                                                                                                                                                           cgcAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CCCGACTGGAGCAGTTTGCCGTCGATGTACCG‑GATATCGGCCCACAATGGG‑CTGCGGATC‑ACGTTTTTTTTCAGATCTTCCCTGATCGgtt                                          >  2:543883/1‑239 (MQ=255)

                                                                                                                                                                                           cgcAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGGCTGGAGCAGTTTGCCGTCGATGGACCG‑GATATCGGCCCACAATGGG‑CTCCGGTCC‑ACATTTTTTTTCAGGTTTTCCCtcacccttt                                          >  2:967141/1‑231 (MQ=255)

                                                                                                                                                                                           cgcAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGCCGTCGATGTACCG‑GATATCGGCCCACAATGGG‑CTGCGGATC‑AGATTTTTTATCAGACCTTCCCTGATCGttt                                          >  2:1015996/1‑239 (MQ=255)

                                                                                                                                                                                           cgcAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGGGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCCCCGG‑CACGACTGGCGCGATTTGCCGTCGATGTCGCG‑GTAACCGGGCCCCAATGGC‑GGGCGGTGC‑GCATTTTTTATCAGactacccctaaaccttt                                          >  1:797037/1‑223 (MQ=255)

                                                                                                                                                                                           cgcAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGAT‑TCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCt                                                                                                                                                           >  2:90782/1‑129 (MQ=255)

                                                                                                                                                                                            gcAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGTCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGATGTGGCACGATCGCCAGCGTT‑GGGTTACCCCGCAGGGCTTCAGCCGAATGCCGCCGG‑CCCGCCTGAAGCAGTTTGCAGTGCCTGTACAGAGGTA‑CTGCCC‑CAATGGGCCTGCGGGGC‑GGTTTTTTTATCAGATCTTccagaagactgt                                          >  1:728640/1‑228 (MQ=255)

                                                                                                                                                                                              cttccgcccccgCCTGGCGTCCCCGCAGGGCGTGCGGCGATTGATTCCTCC‑A‑CCGGACAACCCCGGCTGCGTTACAGTTTCAAACGGGTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGCCGTCGATGTACCG‑GATATCGGCCCACAATGGG‑CTGCGGATC‑AGATTTTTTATCAGATCTTCCCTGATCGTTTTgc                                       <  1:1105819/228‑1 (MQ=255)

                                                                                                                                                                                                tCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGCCGTCGATGTACCG‑GATATCGGCCCACAATGGG‑CTGCGGATC‑AGATTTTTTATCAGATCTTCCCTGATCGTTTTgcgc                                     >  2:96791/1‑239 (MQ=255)

                                                                                                                                                                                                     cGCAGCCTGGCGTCCCCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGCCGTCGATGTACCG‑GATATCGGCCCACAATGGG‑CTGCGGATC‑AGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAg                                 <  2:149514/238‑1 (MQ=255)

                                                                                                                                                                                                          ccTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGCCGTCGATGTACCG‑GATATCGGCCCACAATGGG‑CTGCGGATC‑AGATTTTTTATCAGATCTTCCCTGACCGTTTTGCGCGTAGTCtt                             >  2:1238157/1‑237 (MQ=255)

                                                                                                                                                                                                            tGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGCCGTCGATGTACCG‑GATATCGGCCCACAATGGG‑CTGCGGATC‑AGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTc                         >  2:3819/1‑239 (MQ=255)

                                                                                                                                                                                                            tGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAA‑CCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGCCGTCGATGTACCG‑GATATCGGCCCACAATGGG‑CTGCGGATC‑AGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGGATTCCTCg                        >  2:1074577/1‑239 (MQ=255)

                                                                                                                                                                                                                   aCCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGCCGTCGATGTACCG‑GATATCGGCCCACAATGGG‑CTGCGGATC‑AGATTTTTTATCAGATCTTCCCTGATCGTTTTGCCCTGAGCCTTCCCCGTGaa                    >  2:1209492/1‑237 (MQ=255)

                                                                                                                                                                                                                         gggggtggggggttttgtctcgcC‑A‑GCGGACAACCCCGGGGGCGTTACAGTTTCAATCTGCGGGGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGCCGTCGATGTACCG‑GATATCGGCCCACAATGGG‑CTGCGGATC‑AGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACa            <  1:615148/222‑1 (MQ=255)

                                                                                                                                                                                                                            cGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGCCGTCGATGTACCG‑GATATCGGCCCACAATGGG‑CTGCGGATC‑AGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGACTTCCTCGTGAAGCTGAACAgg          >  2:1168310/1‑238 (MQ=255)

                                                                                                                                                                                                                             ttGCGGCGGTTGAT‑TCTTC‑ACGCGGACAACCCTGGCGGGTTTCCCGTTTCAAACTGCGGGGGCACCATCGCCAGCGTT‑GTTTTACCCCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGCCGTCGATGTACCG‑GATATCGGCCCACAATGGG‑CTGCGGATC‑AGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGaa        <  1:409649/238‑2 (MQ=255)

                                                                                                                                                                                                                              tgtgcgggttcagctctcC‑A‑CCGGACACCCCCGGGGTGGTTACAGTTTCAAAGTGCTGGGGCCCGATCGCCTGTGTT‑GGTTTACACCGCAGGTCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGCCGTCGATGTACCG‑GATATCGGCCCACAATGGG‑CTGCGGATC‑AGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGATc       <  1:858107/226‑1 (MQ=255)

                                                                                                                                                                                                                              tGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGCCGTCGATGTACCG‑GATATCGGCCCACAATGGG‑CTGCGGATC‑AGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTCGTTTTCCTCGTGCAGCTGAACAggtac       >  2:559374/1‑236 (MQ=255)

                                                                                                                                                                                                                               gcggcgTTTGATCTCTCA‑C‑GCGGACAACCCCGGCGTCGTTACAGTTTCAAACTGCTGTTGCACGAGCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGCCGTCGATGTACCG‑GATATCGGCCCACAATGGG‑CTGCGGATC‑AGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGATc       <  2:728640/238‑1 (MQ=255)

                                                                                                                                                                                                                               gcggcgATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGCCGTCGATGTACCG‑GATATCGGCCCACAATGGG‑CTGCGGATC‑AGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGATCa      >  2:550228/1‑239 (MQ=255)

                                                                                                                                                                                                                               gcggcgATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGCCGTCGATGTACCG‑GATATCGGCCCACAATGGG‑CTGCGGATC‑AGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAAAAGGATCa      >  2:149127/1‑239 (MQ=255)

                                                                                                                                                                                                                               gcggcgATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGCCGTCGATGTACCG‑GATATCGGCCCACAATGGG‑CTGCGGATC‑AGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGGGAACATGAACAGGAtaa      >  2:111208/1‑237 (MQ=255)

                                                                                                                                                                                                                               gcggcgATTGATCCCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGCCGTCGATGTACCG‑GATATCGGCCCACAATGGG‑CTGCGGATC‑AGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGATc       <  2:1218923/238‑1 (MQ=255)

                                                                                                                                                                                                                                cggcgATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGCCGTCGATGTACCG‑GATATCGGCCCCCAATGGG‑CTGCGGATC‑AGATTTTTTTTCAGATCTTCCCTGGTCGTTTTGGCGGTGGTTTTCCTCTGGAACATGAAAAGGAt        >  1:1002262/1‑236 (MQ=255)

                                                                                                                                                                                                                                 gccggtttaTCTCTCC‑A‑TGGGACACCCCCGGCGGCGTTACATTTCCAAAGCTCAGCGGCCGGCACGCGAGCTTT‑TGTTAACCGCCCAGGGCTTACACCTAATGCGGCCGG‑CACGACTGGAGCAGTTTGCCGTCGATGTCCCG‑GATATCGGCCCACAATGGG‑CTGGGGATC‑AGATTTTTGATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAACCTGAACAGGATCAt     <  1:452541/230‑1 (MQ=255)

                                                                                                                                                                                                                                    gATTGACCTCTCC‑A‑GCGGACCACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACCCCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGCCGTCGATGTACCG‑GATATCGGCCCACAATGGG‑CTGCGGATC‑AGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGATCATGTc  <  2:607006/238‑1 (MQ=255)

                                                                                                                                                                                                                                     aTTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CAc                                                                                                                                     >  2:354874/1‑109 (MQ=255)

                                                                                                                                                                                                                                     aTTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CAc                                                                                                                                     <  1:354874/109‑1 (MQ=255)

ATCAGATTTTCATCACAGGGGAATTATGATGTTAAATGCATGGCACCTGCCGGTGCCCCCATTTGTTAAACAAAGCAAAGATCAACTGCTCATTACACTGTGGCTGACGGGCGAAGACCCACCGCAGCGCATTATGCTGCGTACAGAACACGATAACGAAGAAATGTCAGTACCGATGCATAAGCAGCGCAGTCAGCCGCAGCCTGGCGTCACCGCATGGCGTGCGGCGATTGATCTCTCC‑A‑GCGGACAACCCCGGCGGCGTTACAGTTTCAAACTGCTGTGGCACGATCGCCAGCGTT‑GGTTTACACCGCAGGGCTTCAGCCGAATGCCGCCGG‑CACGACTGGAGCAGTTTGCCGTCGATGTACCG‑GATATCGGCCCACAATGGG‑CTGCGGATC‑AGATTTTTTATCAGATCTTCCCTGATCGTTTTGCGCGTAGTCTTCCTCGTGAAGCTGAACAGGATCATGTC  >  NC_000913/422490‑422955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`